KR20100092922A - A skin whintening cosmetic composition containing a oriental herb extracts mixture stabilized by nanoliposome - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin whitening containing stabilized nano liposome is provided to ensure whitening effect and to enhance skin penetration with formulation stability. CONSTITUTION: A cosmetic composition for skin whitening contains nano liposome as an active ingredient containing poria cocos wolf, Diospyros Kaki Leaf, Prunus armeniaca L., and Ephedra sinica STAPF. The extract is isolated using water, low alcohol of 1-4 carbon atoms, mixture solvent thereof. The composition suppresses melanogenesis and tyrosinase activity.

Description

A skin whintening cosmetic composition containing a oriental herb extracts mixture stabilized by nanoliposome}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition for skin whitening comprising nano liposomes stabilized herbal extract.

The skin is in direct contact with the external environment and serves as an important barrier to protecting the body from physical, chemical and biological stimuli. However, due to the overexposure of UV rays, the skin loses its original function due to intracellular melanin production, inhibition of tyrosinase activity, and inhibition of antioxidant capacity, and various skin pigmentation phenomena such as blackening the skin. Indicates.

Therefore, researches to improve the skin whitening in the development of cosmetics occupy a very important position. Recently, various skin physiological activities of natural plant components have been searched to develop substances having excellent skin beauty effects, and the use of functional cosmetics such as skin whitening is increasing rapidly. However, most herbal ingredients are not only unstable by themselves, but when applying the cosmetics, there is a big problem in the stability of the cosmetics, such as the settling of the contents, color change.

In order to solve these problems, pharmaceutical, cosmetics and food fields are using liposomes that can stably deliver bioactive components without destroying them, and can contain both fat-soluble and water-soluble bioactive components. Recently, with the development of nanotechnology, research and development on nano liposomes that can maximize skin whitening is being actively conducted.

Salvia miltiorrhiza BUNGE is a perennial herb native or grown in Sanya, northeast China. The phenanthraquinone-based pigments include: tanshinone I purple brown (I), tanshinine II red (II), crypto tanshinone (III), and other tanshinol ) I and II are known. However, the pharmacological action of these components is not known yet, and the soaking solution of Salvia ginseng is known to have a slight inhibitory effect against various skin fungi. It is also used for menstrual irregularities, dysmenorrhea, menopause or postpartum abdominal pain. In this regard, phenanthraquinone pigments are likely to be chemically involved in the endocrine system, and in particular related to the improvement of blood circulation disorder due to clotting factors. On the other hand, Chinese patent No. 107133-A is known for the Chinese medicine for the treatment of hypertension manufactured from gastrodia tuber, ginseng, ginseng root and zucchini, Chinese patent No. 1099995-A natural honey, pig gallbladder, bruise Pills and ointments are known as Chinese medicines for the treatment of hypertension made from mung bean starch, saffron, samchimus and powdered gastrodia tuber. However, although these drugs have been described as having a therapeutic effect on hypertension 100, they are not a drug extracted from active ingredients but can be prepared in various forms such as injectable preparations, and cannot confirm the daily dosage of the active ingredients. Also, depending on the region in which the medicinal herbs and preparations are grown or reared, there are problems that are not useful drugs that can be generally used because side effects cannot be identified. Salviae Miltiorrhizae BGE is the dried root of perennial herb, belonging to Labiatae, which contains tanshinone I, IIA, and IIB, 15,16-dihydrotan. Sinon I (15,16-Dihydrotanshinone I) and Cryptotanshinone are known to contain (Information Sup, Shin Min Kyo; Hyangjedae, Yeonglimsa, pp860-862, 1998). The pharmacological actions that have been identified so far for salvia include the prevention and improvement of ventricular vascular disease (Akira Yagi et al .; Planta medica, 55, pp51-54, 1989), the anticancer effect (Yoon YS et al .; Journal of Ethnopharmacology, 68 , pp 121-127, 1999; Jin Liu et al .; Cancer Letters, 153, pp85-93, 2000), antioxidant effects (Kang HS et al .; Arch. Pharm. Res., 20, pp 496-500, 1997), Anti-inflammatory effects (Kang BY et al .; Immunopharmacology, 49, pp355-361, 2000) and the like are known.

Dansam is a perennial herb and has a double hair all over the plant, and it has a tan chestnut compound, a phenanthrachinone-based pigment, at the root. About the efficacy of dansam, the herb wood is described as 丹 蔘 味苦 生 新 能 破 積 調經 除 帶 崩 (dansam is unprecedented. It is good for life, crushes redness, smoothes the hardness, and eliminates stalks). And envelops into the blood, and hydrated water (鹹水), erosion, erosion, and taking a long time is a lot of eye drops, and one of the effects of salsamtang equivalent to the effect of Samultang.

The bilberry extract contains 5-15% of arbutin, which inhibits the action of tyrosinase and contains methyl arbutin, but it is easily hydrolyzed to form D-glucose and hydroquinone, which inhibits the whitening effect. Tannins contained in% by weight prevent the degradation of arbutin and methyl arbutin, and the whitening effect is enhanced. Flavonoids prevent the formation of melanin polymer and slightly inhibit tyrosinase, and triterpenes containing 0.5% ) Acts like a cortisone to inhibit the proliferation of melanocytes and express a whitening effect.

Bilberry grows mainly in cold places such as Mt. Baekdu, and is a medicinal plant rarely found in rocks or wetlands of high mountains in some northern parts of South Korea such as Hongcheon-gun, Gangwon-do.

The bilberry, an evergreen herbaceous plant of the azalea family, grows about 20-30cm tall, and white or light pink flowers bloom in June-July. The round fruit is juicy and ripens in September-October with a strong sour taste. Leaf leachate is used to treat diuretics, rheumatism, gout, etc. to treat kidney stones, and fruit is used for pain relief and infectious diarrhea.

Gold is native to the continent of East Asia, and is found in the mountains of Korea. It is often grown in the field and is about 60 centimeters high. Hairs all over, stems are dense and square, with many branches. Leaves are opposite, few petioles, 4.5 cm long, 8 mm wide, without sawtooth at edges. Flowers attach to gunshot inflorescences, purple, end of main stem and branch. Calyxes are bell-shaped, closed after flowering, corollas are lip-shaped, and corollas are about 2.5 centimeters long. Fruits are inside calyx, rounded.

Huangshan is the root of the perennial herbaceous plant, Cooptis Chinensis FRANCH. The active ingredients are known as Berberine, Copticine, Warenine, Palmatine and the like. The taste is bitter and cold. Efficacy is a metabolic action (瀉火) action is excellent treatment response to any heat-related diseases.

For example, infectious fever and typhoid fever is remarkable, and fever is abundant and the chest burns and thirst relieves severe symptoms. The effect on the burn or burn is because the heat is disinfecting and the wound is easily healed. The fever builds up in the body, causing eye layer fever or increasing blood pressure. In addition, when washed with contaminant water, etc., the sterilization and antipyretic effect will be obtained. Small doses are good for promoting digestion, and gastrointestinal inflammation, which makes the chest feel stuffy and bloated, causing head soreness, and also helps to relieve palpitations caused by nervousness. In addition, the healing rate is high in stomatitis, sulphitis, and stomatitis caused by heat. In the summer of dysentery and diarrhea, diarrhea, while stopping the diarrhea, pulmonary tuberculosis, hemorrhage, nosebleeds, bleeding and bleeding is also effective for symptoms of fever, diabetes, and pertussis and sore throat is effective. The bactericidal activity is remarkable and widely used for skin infections. Animal testing showed antibacterial, anti-inflammatory, antipyretic, bile secretion, and blood pressure lowering.

Yulmu is an annual herbaceous plant belonging to the rice family. The scientific name is Coix lachryma-jobi var. mayuen (ROMAN STAPF). Origin is known as Southeast Asia or China and is grown. Its height is 1 ~ 1.5m and it grows straight and is divided into several parts. Leaves are alternate, lanceolate, rough, green. Flowers bloom in July and fruits are eaten.

In Korea, there was a record that came from Song Dynasty in 1078 (Moonjong 32), and it was cultivated early in the Joseon Dynasty due to the Japanese record that Kato took it from Japan to Japan. It is assumed to have been cultivated. Cultivation techniques include seedling cultivation and direct cultivation. Seedling cultivation is carried out in the north central region where growth days are short. From mid-April to late April, seedlings are lined up between 10cm and seedlings are planted at intervals of 5cm. Raising seedlings takes 25 to 30 days and leaves 4 · 5 leaves are set up at the main branch from early May to mid May.

Manure is made of manure 1,000 kg, 10 kg of phosphoric acid and 10 kg of Kali per manure per 10a. Straight cultivation is dotted or planted at a planting density of 60 × 10-20 cm in late April to early May. Just before and 2 weeks after the oozing, the skin is given more nitrogen and the clearing area is phosphate. Harvest is done when the fruit becomes dark brown in September to November.

In the past, it was used as grain and ate a lot. Recently, the effects and tonic effects on adult diseases have been noted, and has been used as processed foods such as Yulmucha. In oriental medicine, Ui-in (薏苡仁) is used as a medicine. It is effective for the symptoms of diarrhea, whole body edema, muscle spasms, dyspepsia, and the like due to the effect of dry rain, biliary tract, clear fever, and diarrhea. The leaves and roots are also used as medicine.

Baekbokyeong Pokryeong (Poria cocos) is a fungus that grows from the roots of pine, and has a ball shape or oval with a diameter of 5-7cm, and is brown or dirty black with bark pieces on the ground. Corkized, hard and covered with a thickness of 0.2-0.5 mm. The contents are white or pink and cracked radially. Almost tasteless and odorless, sometimes spreads with mild mucus, positive for iodine. It is said that Fukryeong has been known to make Giran healthy, stabilizer, and protect the body, and to warm the body. It is effective for peptic ulcer, muscle spasms, thirst, vertigo, mental anxiety and insomnia. It is effective in tonic, diuresis and sedation as a herbal medicine, and has been used for kidney disease, cystitis and urethritis. In addition, the effect of inhibiting contact dermatitis has been found. A study on Poria cocos was developed to explore and quantify the indicator components for the standardization of the quality of Bokryeong, such as Hwang-lam [Korean Journal of Pharmacognosy, Vol. 36, No.3, 177p, 2005], Changes in Color and Chemical Compositions of Bokryeong According to Drying Methods of Paper Type [Korean Food Science Society, 1999] Journal of Food and Nutrition Studies, Vol. 9, No. 4, 438p, 1996], but studies on the components of Bokryeong have been reported, but studies on the physiological activity of Poria cocos have been insufficient.

Poria cocos wolf is a plant from the family of pines and is like a mushroom that is parasitic at the roots of pine and Chinese red pine, and according to Dong Bogam, it has a uniform, tasteful, non-poisonous, open stomach, thinning area To soothe the lungs and the mud, treat the shrine, stool, swell, swell, and kill, sogal and dryness. It has been described as having a healing effect, and "pharmaceutical (medicinal drug) metabolism" and "pharmacology, ingredients, clinical application of Chinese medicine" is the abdomen, antibacterial action, hypoglycemic action, gastric acid, heart contraction strengthening effect, etc. It is described as having a variety of actions useful for.

Diospyros Kaki Leaf is a leaf of persimmon, which is a dicotyledon. The persimmon tree grows about 6-14m high, the outer shell of the stem is split into scales, and the twig has brown hairs. Leaves are alternate, leathery, oval-shaped. Leaves are 7-17cm long, 4-10cm wide, without teeth, and petioles are 5-15mm long, with hairs. The back side of the leaf is green and glossy. Persimmon leaves include tannin, vitamins C, P, B1 and B2, carotene, essential oils, organic acids and fiber. Persimmon leaves are widely used as a folk remedy. Persimmon leaf decoction contains a lot of vitamin C and P to prevent blood pressure rises in advance and reduce the amount of cholesterol in the blood. In addition, there is a lot of alkaline components to clear the blood, increase the immunity of lymphocytes and enhance the phagocytosis function of white blood cells to increase the resistance of the organism. Eating persimmon leaves for a long time helps to treat diabetes, high blood pressure, constipation and stomach disease, and is said to be effective for head ache, rashes, eczema and allergic acne.

Apricot Seeds Apricot is a dicotyledonous plant and is a deciduous arborescent of the Rosaceae family. Fruits are nucleus, round, hairy, 3cm in diameter, ripen in yellow or yellowish red in July. Fruits are called algae (杏仁) in oriental medicine, which contains 35 to 50% by weight of oil, and others are amigdaline (aka amigalloxide, colorless crystalline glycoside, C ₂₀ H ₂₇ O ₁₁ N), emulsin (Enzymes), proteins, and the like. The main component of oil is olein. Apricots are used to treat coughs and asthma in one shot. An ingredient called amigdaline in oil extracted from apricot seeds is known to have a good effect on asthma. Agreement says: 'Apricot seeds are used when the coughing is causing shortness of breath, shortness of breath and sputum swells. Apricot seeds act as antitussive expectorants. Cut the tip of the seed, roast it a bit, and then mix it with honey. A lot of fat is also used as a remedy for constipation. Treat constipation and postpartum constipation caused by the dryness of the elderly or the weak. In the United States, apricot seeds are known to have the effect of inhibiting cancer and are also used as anticancer drugs. However, since the amidaline is a toxic substance, it can be in danger if you eat a lot at once. Amigalin is decomposed by the enzyme glucronidaze in the body to produce liquidation, so it is harmful to the human body if consumed in large amounts. The content of amigdalin varies greatly depending on the variety and growing conditions (single apricot seed 0.11% to bitter apricot seed 6%), but is often 3% higher than peach seeds. In addition, amigdaline is hydrolyzed by emulins, amidalases, prunases, oxynitrilases. Initially, it is converted to prunasine (d-mandelonitrile glycoside, C ₁₄ H ₁₇ O ₆ N) and glucose by amigdalase, and prunasin to mandelonitrile (C ₈ H ₇ ON) and glucose by prunase. do. Mandelonitrile becomes benzaldehyde (C ₆ H ₅ CHO) and hydrogen cyanide (HCN) by oxynitrilease or snail digestive enzymes. Hydrogen cyanide has a sedative effect on the respiratory tract, but it is a substance that should be used to control the amount of use because it causes poisoning in large amounts. Therefore, it is safe to eat less than ten apricot seeds at one time. If you eat a lot of dizziness, fainting, headache, chills, vomiting or difficulty breathing. Severe deaths are possible. In children it is more sensitive. When addicted to apricot seeds, the bark or root of the apricot tree is used as an antidote.

Apricot seeds have been used as a raw material for various products such as cosmetics and soaps, or have been medicated for people with poor lungs, such as asthma, by folk remedies. However, as described above, substances such as amidaline contained in the skin, endothelial and eye of apricot seed have an undesirable effect on the human body when ingested in a large amount, and thus there is a limit in processing apricot seeds into food.

The KFDA excludes apricot seeds from food items for the above reasons.

Apricot seed oil is an oil extracted from the seeds of the Prunus armeniaca or Prunus mandshurica. Representative active ingredients include fatty oil, Amygdalin, proteins and various amino acids, Amygdalose, Prunase, Pruansin and the like. Apricot seed oil used in the present invention has a pale yellow color as a fatty oil obtained by purifying and compressing apricot seeds, and the carbon composition of the alkyl group was oleic acid or linoleic acid.

Apricot seed (Prunus armeniaca L.) belongs to the family Rosaceae and is found in the Herb Gardener. Stop coughing, sputum, asthma, and greasy, smooth bowel, so that you have a good bowel movement. Its main components are about 3% of amidaline, about 50% of fat oil, protein and various free acids, citric acid and malic acid, etc., and are particularly rich in potassium and phosphorus, and include sugars, calcium, sodium, fiber, vitamins A, B, It contains C.

Ephedra is a perennial shrub herbaceous plant, called Ephedra, Ephedra, and Ephedra. The scientific name is Ephedrae Herba, which is used to cut green thin branches before and after autumn and cut them in the shade before use. Ephedra sinica is a plant belonging to the Ephedraceae and has been used for thousands of years as a sympathetic stimulant all over the world. In particular, ephedra has been used in the treatment of obesity in anticipation of the effects of sympathetic nervous system excitement, appetite suppression, heat production and metabolism increase, exercise performance improvement. In many literatures, the weight loss effect of ephedra has been recognized and gained popularity as a major component of weight control dietary supplements in the United States until its use as a food. The most widely used prescriptions for the treatment of obesity in oriental medicine were Saeum-jeuptang and Jowiseungcheongtang as sasang prescriptions. Ephedra contains about 0.3 ~ 1% of alkaloids, and the main component of alkaloids is L-ephedrine, which contains several ephedrine compounds such as norephedrine, (+)-pseudoephedrine, and norpseudoephedrine. . In addition, there is a volatile benzyl methylamine and a small amount of essential oil, and the extract contains potassium and calcium. L-ephedrine, the main ingredient of ephedra, was first extracted in 1885. Among the 40 types of ephedra found in Asia, Europe and the United States, the highest alkaloid component is found in Asian species, including Ephedra sinica, which is used in Korea. Became known.

On the other hand, in oriental medicine, medicine is a kind of therapy that regulates the body's overall function, increases resistance, and replenishes energy, and is a natural product that plays a role in encouraging blood-related substances such as blood and energy It is an extract of.

Ephedra sinica STAPF is a perennial herb and the main components of ephedra are ephedrine, pseudo-ephedrine, 1-N-methylephedrine, 1-norephedrine, dN- pseudomethylephedrine and d- Demethyl-pseudo-ephedrine, ephedine and the like, nonacosanol, nonacosane, triacontaol and the like. Ephedra essential oil has been reported to have an inhibitory effect against influenza virus in vitro and to be cured by subcutaneous injection in mice infected with type A or PR type ₈ influenza virus. Jeo Bok-seop, Doha Hyangje Metabolism (Plant), Younglimsa, 1998). In addition, ephedrine, a representative substance of ephedra alkaloid, is known to have antipyretic, anti-inflammatory, bile secretion, as well as cardiovascular system, and has been used for sweating, asthma, and cold.

In the conventional cosmetic field, as a whitening component, substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, hydroquinone, vitamin-C (L-Ascorbic acid), and derivatives thereof Various plant extracts have been used.

However, the whitening ingredients are poor in stability in the prescription system, and are degraded or colored, or their use is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems. For example, kojic acid inhibits enzymatic activity by adsorbing copper ions present in the active site of tyrosinase, but when used in cosmetics, instability, problems of skin side effects, and recent animal experiments have revealed that liver cancer is ceased. It became. Vitamin-C and its derivatives are difficult to use as raw materials for cosmetics due to their oxidative instability, and hydroquinone has excellent whitening effect on skin but allergic properties, toxicity to melanocytes, permanent depigmentation to skin, etc. It is highly irritating to skin, and is recently prescribed as a carcinogenic substance, so its use is banned and only a limited concentration is allowed in each country. In addition, arbutin is a derivative in which glucopyranoside is combined with hydroquinone, and has little side effects when using hydroquinone and inhibits the synthesis of melanin pigments without toxicity to humans, thereby increasing melanin pigmentation. Although it has been suggested that it can be used as a therapeutic agent for skin diseases, it is disadvantageous to be partially degraded by skin enzymes.

In addition to these whitening materials, researches have been conducted to find whitening active ingredients among natural products, especially plants, among them (Japanese Patent Application Laid-Open No. 2-207028, Korean Patent Publication No. 96-033442), and Aloe (Japanese Laid-Open Patent Application). 52-44375, Hei 2-207030, Hei 5-139950), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Laid-Open Patent No. 92-002111), Cinnamon (Japanese Laid-Open Patent No. 63-30403, Hei 5-139954), Licorice (Japanese Patent Laid-Open No. 60-214721, Japanese Laid-Open No. 60-214728, Japanese Laid-Open No. 63-23809, Korean Patent No. 64-63506, Korean Patent Publication No. 1-149706 and Korean Patent Publication No. 92-002109) , No. 97-025601), Sangbaekpi (Japanese Patent Nos. 55-44375, No. 64-26507, No. 64-83009, No. 1-25687, No. 5-139950 and No. 92-002109 No. 97-021273), Gosam (Japanese Patent Laid-Open No. 64-26507 and Korean Laid-Open Patent No. 92-002110), Brown root (Japanese Laid-Open Patent No. 60-214727, No. 64-16709), Angelica ( Japanese Patent 56-92211, Pyeong 4-26610), Mokdanpi (Japanese Patent Publication No. 60-214721, So 61-50915, So 61-246109) and many plant extracts and herbal extracts act on tyrosinase It has been found that it suppresses the production of melanin, but they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration, etc., and still does not show an excellent effect.

The present inventors have made diligent research efforts to develop cosmetic compositions for skin whitening, that is, skin protection using herbal medicines in folk medicine and herbal medicine, and as a result, extracts of salvia, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra It has been found to have a good bioactive effect on whitening. In addition, the use of the cosmetic composition consisting of the herbal composition is effective in inhibiting melanin production, inhibiting tyrosinase activity, and improving the antioxidant ability, and the active ingredients are kept stable in the cosmetic composition by nano liposomes of the extract, In addition, it was confirmed that the physiological activity effect is maximized. Therefore, an object of the present invention to provide a cosmetic composition for skin whitening comprising nano liposomes stabilized herbal extract as an active ingredient.

Salvia red ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra extract according to the present invention can be prepared as follows.

Salvia red ginseng, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra seed, ephedra extract, golden ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, Persimmon leaves, apricot seeds and ephedra are dried in a cool place and then physically pulverized, and then extracted by adding an extraction solvent to the pulverized product. Examples of the extraction solvent include distilled water, anhydrous alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol), acetone, ethyl acetate, chloroform, glycerin, butylene glycol, propylene glycol, dichloromethane, hexane and benzene. At least one selected may be used alone, or two or more solvents may be mixed and used. The ratio of the pulverized product and the solvent is 1:10 to 1:30, preferably 1:20. It is extracted in the extractor and freeze dried.

On the other hand, it is effective that the salviae, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra extract is contained 0.001 ~ 30.0% by weight, preferably 0.1 ~ 10.0% by weight relative to the total weight of the cosmetic composition. . When the content of the Dansam, tangerine extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract is less than 0.001% by weight, it is difficult to expect the effect, if it exceeds 30.0% by weight There is no increase in effect.

Salvia Miltiorrhiza, Bilberry Extract, Golden, Rhubarb, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed, Ephedra Extract according to the present invention reduces melanogenesis by melanogenesis cells (Table 5) and inhibits tyrosinase activity (Table 5). 6) It has a whitening effect. In addition, there is an antioxidant effect (Table 8, Table 9) to eliminate free radicals, and less cytotoxicity (Table 7).

Therefore, the extract of red ginseng, bilberry, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract of the present invention can be effectively used as a whitening composition for inhibiting excessive melanin production. In addition, it can be usefully used for antioxidants in various products. The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar functions in addition to the salviae, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra extract.

Cosmetics made of a cosmetic composition containing the salviae extract, bilberry extract, golden, yellow lotus, jujubeum, baekbokyeong, persimmon leaf, apricot seed, ephedra extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. . Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, packs, etc., and cosmetics of the solubilized formulations are soft cosmetics. In addition to the cosmetics containing the salviae, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract of the present invention, by using a dermatologically acceptable medium or base to be commonly used in the field of dermatology It may be prepared in the form of adjuvants which can be applied topically or systemically.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, a cream, a skin, a lotion, a powder, an ointment, a spray, or a conceal stick.

In addition, the cosmetic composition of the present invention is in addition to fatty acid, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, in addition to Salvia soybeans, bilberry extract, golden, yellow lotus, Yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract , Suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, It may contain adjuvants commonly used in the field of cosmetics or dermatology, such as dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Skin Whitening Salvia Militiorrhiza, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed, Ephedra Extract

Salvia ginseng, bilberry extract, gold, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra of the present embodiment was used in Korea.

The leaves and fruits of Salvia red ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds and ephedra, are dried in a cool shade. Then, using a grinder, finely pulverized dried Salvia ginseng, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra, 200g of the pulverized powder in a reflux extractor, pulverized Salvia ginseng, bilberry extract , Golden, rhubarb, yulmu, baekbokyeong, persimmon leaves, apricot seeds, 70 times the weight of ephedra, 70% ethanol is added and extracted by heating at 75 ℃ for 10 hours. The extract was filtered with a 400 mesh filter cloth, and the filtrate was collected and freeze-dried.

Preparation Example 2 Preparation of Nanoliposomes Containing Herbal Extracts

The nano liposomes were added to phase A in the composition of Table 1 below, uniformly wet, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high-pressure homogenizer 2-4 times under conditions of 600-1000 bar and cooled to obtain a nano liposome that stabilized the herbal extract having a particle size of 30-70 nm in diameter.

Prize ingredient Content (% by weight) A Propylene glycol 40 B Ceramide 2.5 cholesterol 2.5 Polysorbate-80 0.5 Sodium Stearoyl Lactate 0.5 Stearic acid 0.5 lecithin 5.0 C Potassium hydroxide Quantity Herbal Extract (Solid) 10.0 Purified water To 100.0

Examples and Comparative Examples

Compositions containing nanoliposomes stabilized with the herbal extracts of the present invention (Examples) and compositions without nanoliposomes (Comparative Examples) were prepared in the following compositions.

ingredient Example Comparative example Content (% by weight) Content (% by weight) Nanoliposomes (Table 1) 10 - Herbal Extract (Solid) - 10 A Squalane 5.0 5.0 Liquid paraffin 3.0 3.0 Polysorbate 1.2 1.2 Sorbitan stearate 0.5 0.5 Tocopherol Acetate 0.2 0.2 BHT 0.05 0.05 Micromethicone 3.0 3.0 Trioctanoine 5.0 5.0 Microcrystalline Wax 0.7 0.7 Glyceryl Stearate 1.5 1.5 Cetostearyl alcohol 2.0 2.0 B 1,3-butylene glycol 2.0 2.0 glycerin 4.0 4.0 EDTA-2Na 0.05 0.05 Purified water To 100 To 100 Incense, preservative

As described above, the present invention provides a cosmetic composition for skin whitening comprising a nanoliposome containing the herbal extract as an active ingredient. The cosmetic composition of the present invention improves skin whitening through inhibition of melanin production and inhibition of tyrosinase activity. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nano liposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Experimental Example 1: Skin Penetration Effect

The skin penetration effect on the cosmetic containing nanoliposomes stabilized herbal extract of the present invention was measured. Dermal Equivalent (DE) -human normal fibroblasts 1 X 10 5 cells / mL in a gel structure similar to that of the human connective tissue 3 mg / mL: 5 X DEM: 2: 2 sodium bicarbonate and 200 mM 0.05 N sodium hydroxide containing Hepes buffer was inoculated in a medium mixed with 7: 2: 1 and incubated at 5CO ₂ , 37 ° C for 7 days. It is placed inside the plate separated by the inside and the outside by a membrane made of 3 μm porous polycarbonate material, and epidermal Keratinocyte on the surface of Derma Equivalent (DE) where the fibroblasts were cultured. ) Inoculated with 1 X 10 5 cell / ㎖ cells and cultured for 7 days in the inner and outer K-SFM medium containing EGF and BPE. Afterwards, the medium inside the plate was discarded to allow the air to come into contact with the surface of the cultured cells. The medium was mixed with K-SFM containing 10FBS and DMEM without EGF in the same amount and incubated for 2 weeks. Artificial skin with dermis was obtained. The experimental and comparative examples of the present invention were applied to the artificial skin tissue of the top layer, and after 4 hours of incubation, the amounts of KS FM and water containing 10FBS were analyzed by HPLC. The test results are shown in Table 3 below.

Amount of herbal extract in the medium (µg / ml) Example Comparative example 41.5 3.0

Experimental results As shown in Table 3, it can be seen that the cosmetic containing the nanoliposomes stabilized herbal extract of the present invention is 13 times more effective skin penetration than the comparative example using the herbal extract as it is. This is because the average particle size of the nano-liposomes of the present invention is very small, 30-70 nm, so that skin penetration is easy.

Experimental Example 2: Stability Test of the Formulation

The stability test for the cosmetic containing the nanoliposome stabilized herbal extract of the present invention was carried out by the following method. To test the stability of the cosmetics, the examples and comparative examples were stored in an opaque glass jar in a constant temperature bath at 45 ° C. for 12 weeks, and also in an opaque glass container in a completely shaded refrigerator kept at 4 ° C. After storage for 12 weeks, the degree of separation and discoloration were measured. The results are summarized in Table 4 below. At this time, the degree of product separation and discoloration were evaluated by classifying into the following six grades.

0: no change; 1: very little separation (discoloration); 2: a little separation (discoloration); 3: A little severe separation (discoloration);

4: severe separation (discoloration); And 5: very severe separation (discoloration).

Temperature Separation and discoloration Example Comparative example 45 ℃ 0 5.2 4 ℃ 0 0

As can be seen in Table 4, the Example was stable without discoloration or separation at 45 ℃, the comparative example used for direct formulation without stabilizing the herbal extract is severe separation and discoloration at 45 ℃ due to the unstable extract In addition, it can be seen that the herbal extract entered the formulation and the formulation itself became unstable. Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing a nano liposome stabilized herbal extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Experimental Example 3 Intracellular Melanin Inhibitory Effect

B16F1 melanoma cells were provided by a Korean cell line bank and cultured. B16F1 melanoma cells are cell lines producing melanin and are generally used for screening whitening substances.

The medium for culturing the cells was sterile by adding DMEM, 10% FBS, 1% penicillin / streptomycin.

Cell cultures were incubated in an incubator at 37 ° C., 5% CO ₂ . Melanoma cells were cultured and passaged when the 100 mm Petri dishes were about 90% full.

Changes in the amount of melanin produced by treatment with B16F1 melanoma cells were measured to determine the whitening effect of Salviae, Bilberry extract, Golden, Huanglong, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed and Ephedra extract. Cells were seeded on 6-well plate and treated with Salvia soybean, Bilberry extract, Golden, Rhubarb, Yulmu, Baekbokryeong, Persimmon Leaf, Apricot Seed, and Ephedra extract prepared in Preparation Example 1 for 200 days. The cells were recovered by culturing. The recovered cells were added with 1N NaOH containing 10% DMSO, reacted at 90 ° C for 1 hour to be sufficiently dissolved in melanin, and the absorbance was measured at a wavelength of 405 nm using an ELISA reader.

Experimental results were calculated using the following equation, the inhibition is shown in Table 5. Arbutin, which is known as a general whitening substance, has a melanin content of 59.6% at a concentration of 200 µg / ml compared to the untreated group, whereas salvia, bilberry extract, golden, rhubarb, radish, baekbokyeong, persimmon leaves, apricot seed, ephedra The extract was 68.2% and it was confirmed that it had a similar melanogenesis inhibitory effect as Arbutin.

Sample Name Untreated Arbutin Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus Melanin Production (%) 100 69.6 68.2

Experimental Example 4 Measurement of Tyrosinase Activity Inhibition

It was determined whether the extracts of Salvia Miltifolia, Bilberry, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed, and Ephedra extract affect the tyrosinase enzyme activity, which plays an important role in producing melanin.

20ul of 0.1M phosphate buffer (pH 6.8) was added to the 96 well plate and 200µg / ml of Salvia Militiorrhiza, Bilberry Extract, Golden, Rhubarb, Yulmu, Baekbokryeong, Persimmon Leaf, Apricot Seed, Ephedra Extract or Distilled Water Add 50ul then 10ul of mushroom-derived tyrosinase. After adding 20ul of 5mM L-DOPA as a substrate and reacting for 2 minutes at 37 ℃, the absorbance was measured at a wavelength of 475nm using an ELISA reader.

Tyrosinase activity was calculated using the following formula, and the melanin inhibitory effect of koji acid and the extract, which was widely used as a whitening agent, is shown in Table 6. When Koji acid was treated, only 3.1% of the total activity was active, whereas salvia, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra extract showed about 51.4% activity. Compared to Kojisan, the inhibitory effect is weaker, but salviae, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract also inhibits tyrosinase enzyme activity more than 51.4% compared to untreated there was.

Sample Name Untreated Kojisan Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus Tyrosinase Activity (%) 100 3.1 51.4

Experimental Example 5: Toxicity Test

In order to use the white ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract as a whitening cosmetic, an experiment was performed to check the cytotoxicity. Inoculate the cells in 96 well plate, and treated with 200 g / ml of salvia, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract, remove the medium after two days, and add 100ul of MTT solution After 4 hours of reaction at room temperature, the solution was removed, and 100ul of DMSO was added to dissolve the stained cells, and then the absorbance was measured at 540 nm using an ELISA reader.

Cell viability was calculated using the following formula, and the results are shown in Table 7. Salvia miltiorrhiza, blueberry extract, by gold, goldthread, Coix, baekbokryeong, persimmon, apricot kernel, as compared with cells not treated with extract of Ephedra sinica, 200 ㎍ / high concentration and a ^99.9% cell survival rate in spite of ㎖ It can be seen that there is little cytotoxicity.

Sample Name Untreated   Arbutin Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed Cell survival rate (%) 100 97. ^{3 99.9}

Experimental Example 6 Antioxidant Activity Experiment I

In order to determine if the antioxidant activity of Salvia ginseng, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract was generally measured using the DPPH solution, which is a method of measuring the antioxidant activity.

After mixing 100 g each of red ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra seed, ephedra extract and DPPH solution, an antioxidant measurement solution, were reacted at 37 ° C. for 30 minutes, followed by ELISA reader (reader The absorbance was measured at 570 nm using.

Experiments with vitamin C, known as antioxidants, compared the relative activity of extracts. As can be seen from Table 8, it was found that the antioxidant activity of Salvia soybean, Bilberry, Golden, Huanglong, Yulmu, Baekbokryeong, Persimmon Leaf, Apricot Seed, and Ephedra extract was 79.7%. Could. It is unique that Salvia extract, Bilberry, Golden, Hwang lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed and Ephedra extract showed 4.5 times better antioxidant activity than Vitamin C.

Sample Name Vitamin c Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed Antioxidant activity (%) 17.9 ^percent 79. ⁷ %

Experimental Example 7 Antioxidant Activity Experiment II

NBT test of Superoxide dimutase like activity (Superoxide dimutase like activity) as another method to measure the antioxidant activity of Salviae, Bilberry Extract, Golden, Huanglong, Yulmu, Paekbokyeong, Persimmon Leaf, Apricot Seed and Ephedra Measured by the method.

Add 10ul of NBT solution to a 96-well plate and 40ul of Xanthine as a substrate. Add the red ginseng, bilberry extract, golden, yellow lotus, juvenile radish, baekbokyeong, persimmon leaf, apricot seed, ephedra extract, 25㎍ / ㎖ and phosphate buffer, and add 20ul of enzyme xanthine oxidase. After reacting at 25 ° C. for 20 minutes, the absorbance was measured at a wavelength of 560 nm using a LISA reader.

As can be seen from Table 9, the NBT activity of Salvia soybean, Bilberry, Golden, Huanglong, Yulmu, Baibok-ryeong, Persimmon leaf, Apricot seed, and Ephedra extract was very high at 40. ⁵ %.

Sample Name Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus Superoxide Scavenging Effect (%) 40. ⁵ %

Examples of preparations for the compositions of the present invention are illustrated below.

Preparation Example 3 Example and Comparative Example

To prepare a cosmetic composition containing the extract of Salvia red ginseng, bilberry tree, golden, yellow rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract of Preparation Example 3, and to compare the , Persimmon leaf, apricot seed, ephedra extract lacking a cosmetic composition was prepared in the composition ratio of Table 10 below.

division Content (% by weight) ingredient Example Comparative example Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed 4.0 - Cetearyl Octanoate 5.0 5.0 Isopropyl Palmitate 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 Polysorbate 60 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 Spices, dose dose antiseptic dose dose Purified water Balance Balance Sum 100 100

Experimental Example 8

In order to test the clinical whitening efficacy of the cosmetics of the present invention, 40 healthy adult men and women with blemishes, freckles and pigmentation were randomly divided into two groups (A and B) of 20 people, and then performed on the A group. For example, after applying the comparative example twice a day for 12 weeks for group B, the change in skin color was measured using chromatameter (Minolta CR300), and the change in color brightness (ΔL) was measured. Objective visual observation by and subjective visual observation by subject were performed to measure the whitening effect. The results are shown in Table 11 below. At this time, the degree of whitening efficacy was classified into the following seven ratings.

* Evaluation criteria for whitening efficacy:

-3: very bad, -2: worse, -1: slightly worse, 0: no change, 1: slightly improved, 2: improved, 3: very improved,

Subject Change in brightness of the subject's skin color (△ L)  Objective evaluation of the skilled person Subjective Evaluation of the Subject A group B group A group B group A group B group One 5.6 2.6 3 0 0 One 2 4.6 2.8 3 0 3 0 3 4.5 2.3 2 0 2 One 4 4.3 1.9 2 0 3 One 5 4.8 2.2 2 0 One -One 6 5.1 2.5 3 One 2 -One 7 4.5 0.8 2 One 2 One 8 4.1 0.2 3 One 2 One 9 4.7 2.6 2 One One One 10 4.9 2.8 2 0 2 0 11 4.3 3.5 2 One One One 12 3.8 3.8 2 0 One 0 13 4.2 2.5 3 0 2 One 14 4.8 2.6 2 One 2 One 15 4.2 2.3 2 One 2 One 16 5.6 3.4 3 One 0 0 17 5.7 2.8 3 0 2 0 18 5.3 2.3 2 One 2 One 19 5.3 3.1 One 0 One -One 20 5.2 2.8 3 2 One One Average 4.78 2.49 2.35 0.55 1.6 0.45

As shown in the results shown in Table 11, the change in skin brightness was twice as effective in applying the Example compared to the Comparative Example, and the objective evaluation of the skilled person and the subjective evaluation of the subject were also applied when the Comparative Example was applied. In comparison, the improvement was 4.78 and 2.49 times higher.

As a result, the fact that can be confirmed through the results shown in Table 11, the cosmetics containing the salvia soybean, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds, ephedra extract prepared by the preparation example according to the present invention It can be seen that the excellent effect as to enhance the skin whitening effect.

[Formulation Example 1]: flexible lotion

 On the basis of the results of the preliminary experimental example, a cosmetic composition containing sweet ginseng, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seeds and ephedra extract is presented.

However, the composition of the present invention is not intended to be limited to the following formulation examples. A preparation example of the flexible lotion containing salvia, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract was prepared as shown in Table 12.

Raw material Content (% by weight) Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed 2.00 Allantoin 0.10 Hydrogenated Castor Oil 1.00 ethanol 30.00 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 1,3-butylene glycol 1.00 Dipotassium glycylizate 0.05 Citrix Acid  0.01 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Balance

Formulation Example 2: Nutrition Cream

A preparation example of nutrition cream containing Salvia extract, bilberry extract, golden, yellow lotus, yulmu, baekbokyeong, persimmon leaves, apricot seed, ephedra extract was prepared as shown in the following Table 13.

Raw material Content (% by weight) Sweet Ginseng, Bilberry Extract, Golden, Yellow Lotus, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed 10.0 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Sodium hyaluronate a very small amount Sorbitan sesquioleate 1.5 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Lipophilic glyceryl stearate  2.0 Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Balance

Formulation Example 3 Massage Cream

Massage creams were prepared according to conventional methods as shown in Table 14 below.

Compounding ingredient Content (% by weight) Nanoliposomes 1.0 Triethanolamine 0.5 Polysorbate 60 3.0 Propylene glycol 6.0 Sorbitan sesquioleate 2.5 Beeswax 2.0 glycerin 4.0 Tocopheryl acetate 0.1 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Incense, preservative a very small amount Carboxy Vinyl Polymer 0.5 Purified water to 100

[Formulation Example 4] Pack

Packs were prepared according to conventional methods as shown in Table 15 below.

Compounding ingredient Content (% by weight) Nanoliposomes 2.0 ethanol 7.0 Carboxyvinyl Polymer 0.3 glycerin  4.0 Propylene glycol 2.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (7)

Cosmetic composition for skin whitening containing salvia, bilberry extract, golden, yellow lotus, yulmu, bokbokyeong, persimmon leaf, apricot seed, ephedra extract as active ingredients
The method according to claim 1, wherein at least one selected from the group consisting of the salvia, bilberry extract, golden, rhubarb, yulmu, baekbokyeong, persimmon leaf, apricot seed, ephedra extract water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof The above-mentioned polar solvent was added and extracted, The cosmetic composition for skin whitening characterized by the above-mentioned.
The cosmetic composition for skin whitening according to claim 1, wherein the salvia extract, bilberry extract, gold, yellow lotus, yulmu, baekbokyeong, persimmon leaf, apricot seed and ephedra extract are contained in an amount of 0.001 to 30.0 wt% based on the total weight of the cosmetic composition. .
The cosmetic composition for skin whitening according to claim 1, wherein the skin whitening effect of the composition is due to inhibition of melanin production.
The cosmetic composition for skin whitening according to claim 1, wherein the skin whitening effect of the composition is due to inhibition of tyrosinase activity.
The skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence according to any one of claims 1 to 5 Cosmetics for skin whitening comprising any of the following formulas: packs, soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders and eye shadows Composition.
. Cosmetic composition using antioxidant Salvia Militiorrhiza, Bilberry Extract, Golden, Rhubarb, Yulmu, Baekbokyeong, Persimmon Leaf, Apricot Seed, Ephedra Extract.