KR20190113371A - Composition for skin-whitening and anti-winkle comprising extract of Poria cocos, Coix lachrymajobi and Stachys sieboldii fermented using Hericium erinaceum mycelium as effective component - Google Patents

Abstract

The present invention relates to a composition for improving skin whitening and reducing wrinkles, containing, as an active ingredient, a mixture of Stachys sieboldii fermented extract, Coix lachrymajobi fermented extract and Poria cocos fermented extract using Hericium erinaceus mycelium. More particularly, the mixture of Stachys sieboldii fermented extract, Coix lachrymajobi fermented extract and Poria cocos fermented extract prepared at a specific mixing ratio of the present invention has better skin whitening and wrinkle reduction effect than each fermented extract of Stachys sieboldii, Coix lachrymajobi and Poria cocos alone, and thus may be more usefully used for cosmetics, functional health foods, and the like for skin whitening or wrinkle reduction.

Description

Composition for skin-whitening and anti-winkle comprising extract of Poria cocos, Coix containing a mixture of Baekbok-yeong fermented extract, Yulmu fermented extract, and salt-weed fermented extract using roe deer fungus mycelium as active ingredients lachrymajobi and Stachys sieboldii fermented using Hericium erinaceum mycelium as effective component}

The present invention relates to a composition for improving skin whitening and wrinkles containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract using the Roe mushroom fungus mycelium as an active ingredient.

The skin is directly exposed to the external environment among the body tissues, and acts as a barrier between the internal and external environment of the human body, protecting the living body from the surrounding environment by preventing the invasion of external environmental pollutants and microorganisms including chemicals and ultraviolet rays . In addition, the skin color is determined by melanin, hemoglobin (hemoglobin), carotene (carotene), among which melanin plays the most important role. In addition to determining human skin color, melanin performs skin protection such as ultraviolet absorption and free radical scavenger. However, excessive production of melanin due to external environmental changes such as overexposure to UV rays, air pollution, and stress causes pigmentation in the skin, causing melanism, blemishes, and freckles. Blackening of the skin is caused by the response of skin cells to internal and external factors, a representative factor being due to UV exposure. That is, when the skin is exposed to ultraviolet light, tyrosinase is activated, and the tyrosinase acts on tyrosine present in the skin tissue to produce dopa (DOPA) and dopaquinone. In the melanocytes of the skin pigment cells melanocytes (melanosome) is synthesized a polymer called melanin, which is transferred to the keratinocytes (keratinocyte), the keratinocytes of the skin and reaches the skin surface by the keratinization process It protects the skin from ultraviolet rays. Therefore, melanin is an indispensable UV protective agent in the human body, and also serves as an effective free radical scavenger that removes various radicals that modify biological components such as proteins, lipids, nucleic acids, and the like. However, if the melanin is locally excessively synthesized or the skin's physiological function decreases due to skin lesions and aging, melanin is deposited on the skin surface, causing melasma, freckles and various pigmentation. As mentioned above, the cause and mechanism of skin blackening are revealed, and a substance which has an inhibitory effect of tyrosinase, an enzyme involved in the skin blackening process in the manufacture of a whitening cosmetic, is incorporated into the cosmetic, or part of the melanin production process. Methods for reducing the production of melanin by inhibiting the reaction are generally used. Representative materials used for this purpose include chemicals such as ascorbic acid, kojic acid, hydroquinone and plant extracts such as lettuce extract and licorice extract. However, ascorbic acid lacks the inhibitory effect of tyrosinase activity and is not suitable as an inhibitor of melanin production due to the low stability of the molecule itself, while kojic acid is excellent in inhibiting activity of tyrosinase, but discolored when combined with cosmetics, Therefore, not only there is a problem in stability, such as lowering the titer, the skin irritation is large, there is a limit in use, hydroquinone is limited to use in cosmetics due to skin irritation and safety problems. Therefore, attention is focused on the development of new and effective whitening ingredients.

On the other hand, Poria cocos is said to grow in the shape of a hump from the roots of pine trees many years after cutting it. As a medicinal crop with medicinal effects, it was used as a medicinal herb rather than a food, but according to the agreement, it would have been used as a vulcanized food if it was said that it would not make you hungry without eating grain. According to Dongbogam, the taste of Baekbokyeong is sweet, boring, and flat. Thus, it has been used a lot as a tonic since ancient times and also used for recovery after illness. The main component of the Baekbokryeong contains beta-Pachyman and accounts for about 93% of the dry weight. Triterpenoid compounds include pachymic acid, tumulusic acid, 3-beta-hydroxylanosta-7,9 (11), 24-triene-21-oic acid (3-β-Hydroxylanosta-7,9 (11), 24-trien-21-oic acid). Chitin, Protein, Fat, Sterol, Lecithin, Glucose, Adenin, Histidine, Choline, Beta-Pachman Degradation Enzymes, Lipase, Protease, etc. are contained. It has excellent effects on cancer suppression, stomach, tonic, pancreas and kidney. It has been used as a tonic since ancient times. It works on menopause, parenteral, heart, nerve, and bladder to protect the spleen, sputum, and stabilize the mind. The results of pharmacological experiments have been shown to act as a diuretic, lowering blood sugar, sedation, and also known as an immune resurrection.

Coix lachrymajobi ) is a kind of grain that can be boiled and drinked by tea, and is easily accessible to anyone. It is a kind of medicinal herb used in the name of Uiin in Korean medicine. In oriental medicine, Yulmu is the first food that is good for skin. In the private sector, if the freckles suddenly increase after giving birth, they also ate yulmujuk every day. According to the Herb Tree, Yulmu is 'strengthening the spleen'. Coixenolide contained in Yulmu has anti-cancer effects, and there is research showing that coixol in roots has analgesic, calming and antipyretic effects. It is known that it is related to the anti-inflammatory action of COIXOL, antioxidant action to remove free radicals, antiallergic action and antihistamine action.

Stachys sieboldii miq.) is a perennial herbaceous plant belonging to the Lamiaceae family and grows up to 30 ~ 60cm in height. Flowers are fuchsia and bloom in layers, from the middle of May to the upper part of branches and stems. The root part is a tuber part that looks like a bulb and is pulsating with 1 ~ 3cm in length. Root area is reddish and milky. In other names, it is called Gamja, Jeokro, Gamroah, Jijam, Tochungcho, Jigocho, Jiyu, Earthworm, Botapchae, and Wahchae. Efficacy is known to be effective for fever, seawater, pneumonia, poisoning, blood loss, bleeding, boil, inflammation, analgesic, pneumonia, appendicitis, sore throat, mild fever, swelling. It is also known to be effective in preventing senile dementia and improving memory.

Roe Deer Mushroom ( Hericium) erinaceum ) is a fungi belonging to the Coral Mushroom family, which occurs in the hardwoods and trees of autumn hardwoods in autumn, and is known as monkey head mushrooms and bear head mushrooms in China, Yamabushitake in Japan, and the form of snail in Korea. It is known to be similar to roe deer mushroom. These mushrooms are rich in carbohydrates, proteins, amino acids, enzymes, inorganic salts, and vitamins, and are known to be effective in increasing anticancer and immune functions. Recently, they contain various biologically active substances, including ingredients with anticancer effects. It has been reported.

On the other hand, Korean Patent Publication No. 2016-0024525 discloses a cosmetic composition comprising a choseok nap extract as an active ingredient, Korean Patent Publication No. 2004-0090114 discloses an external composition for skin containing a extract of snail mushroom extract. . However, the composition for skin whitening and wrinkle improvement containing the mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract, and Choseok nap fermentation extract using the Roe mushroom fungus mycelium of the present invention as an active ingredient has not yet been disclosed.

The present invention was derived by the above requirements, in the present invention was prepared a mixture of Baekbokryeong fermented extract, Yulmu fermented extract and Choseok fermented extract using the Roe locust fungus mycelium, the melanogenesis inhibitory activity, Ella As a result of confirming the inhibitory effect of statin activity and collagenase activity, the mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract prepared at specific mixing ratios whitened the skin than the fermentation extract of Baekbokyeong, Yulmu and Choseok nap respectively. And this invention was completed by confirming that a wrinkle improvement effect is remarkable.

In order to solve the above problems, the present invention provides a cosmetic composition for improving skin whitening and wrinkles containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and choseok nap fermentation extract as an active ingredient.

In addition, the present invention provides a skin external preparation for skin whitening and wrinkle improvement containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

In addition, the present invention provides a health functional food composition for improving skin whitening and wrinkles containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

Since the mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok sleep fermentation extract prepared at a specific mixing ratio of the present invention has better skin whitening and wrinkle improvement effect than the respective extracts of Baekbokyeong, Yulmu and Choseok sleep, fermentation alone, skin whitening or Cosmetics for wrinkle improvement, health functional foods can be used more usefully.

Figure 1 shows the cytotoxicity through cell viability when treated with B16F10 cells of the mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract prepared using the Roerung beetle mycelia according to one embodiment of the present invention The result is.
Figure 2 is a result of comparing the melanogenesis inhibitory activity of the fermentation extract of each of Baekbokryeong, Yulmu and Choseok nap prepared using the Roeung beetle mycelium mycelium according to an embodiment of the present invention and the mixture prepared according to various mixing ratios to be.
Figure 3 compares the effect of inhibiting the elastase activity of each of the fermentation extract of each of Baekbokryeong, Yulmu and Choseok nap prepared using the locust fungus Mycelium mycelium according to one embodiment of the present invention and the mixture prepared according to various mixing ratios. One result.
Figure 4 compares the inhibitory effect of collagenase activity of the fermentation extracts of each of Baekbokryeong, Yulmu and Choseok nap prepared by using the Roe mung mushroom mycelium according to an embodiment of the present invention and the mixture prepared according to various mixing ratios. One result.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for improving skin whitening and wrinkles containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

In the cosmetic composition according to an embodiment of the present invention, the fermentation may be fermented using a scab mushroom mycelium, but is not limited thereto.

In addition, the mixture may be one containing 15 to 25 parts by weight of Baekbokyeong fermentation extract, 25 to 35 parts by weight of Yulmu fermentation extract and 45 to 55 parts by weight of Choseok fermented extract, preferably 20 parts by weight. Bokbokyeong fermentation extract, 30 parts by weight of yulmu fermentation extract and may include 50 parts by weight of salt-free fermentation extract, but is not limited thereto.

In addition, the Baekbokryeong fermentation extract, Yulmu fermentation extract and choseok sleep fermentation extract may be prepared by filtering the fermentation of each of the Baekbokyeong, Yulmu and Choseok sleep and freeze-dried the remaining residue, followed by hot water extraction, but is not limited thereto. .

In addition, the mixture of the Baekbokryeong fermented extract, Yulmu fermented extract and Choseok fermented extract are

1) sterilizing at 110-130 ° C. for 10-20 minutes by adding 7-12 parts by weight of baekbokyeong, 17-22 parts by weight of yulmu and 12-17 parts by weight of zeolite to 100 parts by weight of medium, respectively;

2) inoculating each of the Roe mushroom fungus mycelium culture medium to each of the ingredients sterilized in step 1) and fermenting at 24 to 26 ℃ for 7 to 12 days;

3) filtering each fermentation product obtained in step 2) and lyophilizing the remaining residue;

4) obtaining each of the lyophilized fermented product of step 3) by hot water extraction at 75-85 ° C. to obtain Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok fermented fermentation extract; And

5) 15 to 25 parts by weight of Baekbokryeong fermentation extract, jujube fermentation extract and choseokmu fermentation extract obtained in step 4), 15 to 25 parts by weight of Baekbokyeong fermentation extract, 25 to 35 parts by weight of Yulmu fermentation extract and 45 to 55 To prepare a mixture by mixing parts by weight of salt-free fermentation extract; may be to include,

Preferably

1) sterilizing at 121 ° C. for 15 minutes by adding 10 parts by weight of Baek-bok-yeong, 20 parts by weight of yulmu and 15 parts by weight of zeolite per 100 parts by weight of medium;

2) inoculating each of the Roe mushroom fungus mycelium culture medium in each medium sterilized in step 1) and fermenting at 25 ° C. for 10 days;

3) filtering each fermentation product obtained in step 2) and lyophilizing the remaining residue;

4) extracting each of the lyophilized fermented product of step 3) by hot water extraction at 80 ° C. to obtain Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok fermented fermentation extract; And

5) 20 parts by weight of Baekbok-Ryeong fermentation extracts, 50 parts by weight of Yulmu fermentation extracts and 50 parts by weight of Choseok fermentation extracts, based on 100 parts by weight, To prepare a mixture by mixing the; may be prepared to include, but is not limited thereto.

In addition, in step 1), the medium is a culture medium for mushroom hyphae, and PDB (potato dextrose broth) medium may be generally used, but is not limited thereto.

In the cosmetic composition according to an embodiment of the present invention, the cosmetic composition for improving skin whitening and wrinkles is an external skin ointment, cream, supple cosmetics, nourishing cosmetics, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair Treatment, Gel, Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, cleansing lotion, cleansing cream, body lotion and body cleanser may be prepared in any one formulation selected from the group consisting of, but is not limited thereto. The cosmetic composition consisting of each of these formulations may contain various bases and additives necessary for the formulation of the formulation and are suitable, and the type and amount of these components can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may further contain one or more skin whitening active ingredients exhibiting the same or similar functions in addition to the active ingredient. Skin whitening active ingredients include kojic acid and derivatives thereof, arbutin, ascorbic acid and derivatives thereof, hydroquinone and derivatives thereof, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-dinitroindazole or Plant extracts such as vine tangerine extract, rice extract, licorice extract, and the like, but is not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier component is animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. And the like can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. Fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, acethionate, an imidazolinium derivative, a methyltaurate, or a sarcosinate. Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a caustic agent, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen agent, a vitamin, a plant extract, and the like. .

In addition, the present invention provides a skin external preparation for skin whitening and wrinkle improvement containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

The external preparation for skin of the present invention may be prepared in any formulation conventionally prepared with reference to the known art. For example, solutions, suspensions, emulsions, softening lotions, nourishing lotions, pastes, gels, creams, lotions, essences, emulsions, packs, face washes, gels, powders, soaps, surfactant-containing cleansing, oils, powder foundations, It may be formulated as an emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, mask pack, spray or powder.

Fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents in order to use the mixture of the Baekbokryeong fermentation extract, Yulmu fermentation extract, and Choseok nap fermentation extract as external preparations for skin (foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic It may contain adjuvants commonly used in the field of dermatology, such as active agents, lipid vesicles or any other ingredients commonly used in external preparations for skin. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

In addition, the present invention provides a health functional food composition for improving skin whitening and wrinkles containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

The composition may be prepared in any one of powder, granules, pills, tablets, capsules, candy, syrups and beverages, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be appropriately used depending on the purpose of use thereof. In general, in the preparation of food or beverages, the nutraceutical composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of dietary supplement. Examples of foods to which the health functional food composition may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, drinks, tea Drinks, alcoholic beverages, vitamin complexes, and the like, and include all health foods in the conventional sense.

In addition, the nutraceutical composition of the present invention may be prepared as a food, in particular a functional food. The functional food of the present invention may include ingredients that are commonly added. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when prepared with a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates can be monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g. dextrins, cyclodextrins, etc.) or sugar alcohols (e.g. , Xylitol, sorbitol, erythritol and the like). The flavourant may be a natural flavourant (eg, taumartin, stevia extract, etc.) and a synthetic flavourant (eg, saccharin, aspartame, etc.).

Various nutritional supplements, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid The carbonation agent etc. which are used for a drink can be contained further. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for explaining the present invention in more detail, it is obvious to those skilled in the art that the scope of the present invention is not limited by them.

Production Example  One. Baikyeongryeong , Law and Cornerstone  Preparation of Fermented Sole Extract and Mixture Mixed with It

The worm fungus mycelium used for the production of Baekbokryeong, yulmu and salt-free fermentation products, when the worm mushroom mycelium grown in PDA medium grows to 5-6 cm, the mycelium at the edges is removed by cork borer. Five mycelia were inoculated into 100 mL of PDB medium using the inoculum and incubated for 7 days in a shaker (25 ° C, 150 rpm).

100 g of Baekbokryeong, 200 g of yeast radish and 150 g of Choseokjam were added to 1 L of PDB medium, and then sterilized at 121 ° C. for 15 minutes using an autoclave. Inoculated with the mycelia of the Roe mushroom fungus mycelium cultured in the above method in a sterile medium for 10 days in a shake culture (25 ℃ and 150 rpm), filtered using a filter paper and the remaining residue was freeze-dried using a freeze dryer. The lyophilized fermented product was extracted with ethanol, hot water, and purified water, and then filtered to prepare fermented extracts of Baekbokryeong, Yulmu, and Choseokjam, respectively.

In addition, the fermented extracts of each of the prepared Baekbokryeong, Yulmu and Choseokjam were mixed at five mixing ratios as described in Table 1 to prepare mixtures.

Mixing Ratio of Baekbokryeong, Yulmu and Chokseok Ferment Extracts Using Roebuck Mushroom Mycelium of the Present Invention sample Mix ratio (% by weight) Roe deer beetle mycelia
Fermented Baekbokyeong Roe deer beetle mycelia
Fermentation rate Roe deer beetle mycelia
Fermentation Cornerstone A 50 30 20 B 40 30 30 C 30 20 50 D 20 30 50 E 10 40 50

Example  One. Baikyeongryeong , Law and Cornerstone  Preparation of Fermented Extracts and Their Optimum Conditions

In order to measure the mycelial growth of algae, snow Cordyceps sinensis, skirt mushroom, roe beet and S. mushrooms according to the amount of Baek Bok-Ryeong, Yul-Moo and Suk-Tap, each mushroom mycelium was incubated for 15 days and the mycelial density was measured. As a result, as shown in Table 2, in the case of shiitake, skirt mushroom and situation hyphae, mycelial densities were higher in order of Yulmu, Choseokjab, and Baekbokryeong. It was not done. Roe beetle mycelia were found in the order of Yulmu, Baekbokyeong, and Choseokpyeong, showing the highest mycelial density among the five mushroom mycelia. The overall mycelial density of Baekbokryeong and Yulmu increased as the amount added, but the mycelial density decreased as the amount added increased.

In addition, in order to investigate the optimum strain culture temperature of the Roe mushroom species, which was the most excellent mycelial density, the mycelial density of 25 days at different culture temperatures was measured. As a result, the mycelial density was high under the conditions of 22 ~ 28 ℃, and 287.8mg and 275.4mg, respectively, under the conditions of 24 ~ 26 ℃. It was confirmed (Table 3).

In addition, the mycelial density was measured in order to confirm the mycelial growth of the Roe beetle mushroom according to the addition amount of Baekbokyeong, Yulmu and Choseok nap. As a result, as shown in Table 4, it was confirmed that the addition of Yulmu showed the best effect on the mycelial growth of the scaber mushroom. When 200g of Yulmu was added, the density of mycelia was increased to 304.1g. The optimum content of medicinal plants was 100g of Baekbok-ryeong and 150g of Choseokseok, and the mycelial density of 224.4 mg and 246.8mg, respectively.

Measurement of Mycelial Density by Mushroom Type According to the Addition of Medicinal Plants Amount
(g / L) Mycelial density (mg / 15 days) Elevation Snow Flower
Cordyceps sinensis Skirt Mushroom Roe deer situation Baikyeongryeong 50 41.2 ± 4.1 - 141.2 ± 7.4 194.3 ± 6.1 44.2 ± 5.1 100 64.4 ± 5.6 - 131.1 ± 3.2 224.4 ± 7.7 57.1 ± 6.2 Rule 50 150.2 ± 5.7 31.7 ± 2.1 176.4 ± 6.7 214.8 ± 4.7 89.4 ± 3.9 100 164.4 ± 6.1 51.4 ± 3.6 193.2 ± 4.7 239.5 ± 9.2 110.4 ± 3.7 Cornerstone 50 145.2 ± 2.4 12.1 ± 1.4 167.2 ± 2.6 192.1 ± 3.4 72.3 ± 2.6 100 131.3 ± 4.4 17.4 ± 2.6 152.1 ± 6.1 192.1 ± 3.4 64.1 ± 1.6

Measurement Results of Mycelial Density of Roebuck Mushrooms with Different Fermentation Temperatures Temperature (℃) Mycelial density (mg / 25 days) 16 184.5 ± 9.2 18 194.8 ± 8.7 20 221.5 ± 11.2 22 251.6 ± 7.4 24 287.8 ± 8.4 26 275.4 ± 5.7 28 251.7 ± 6.1 30 226.1 ± 9.3 32 197.6 ± 8.2

Measurement Results of Mycelial Density of Roe Beetle Mushrooms with Various Additions Medicinal Plant Content
(g / L) Mycelial density (mg / 25 days) Baikyeongryeong Rule Cornerstone 30 181.7 ± 7.2 191.2 ± 5.4 175.4 ± 7.2 50 194.3 ± 6.1 214.8 ± 4.7 192.1 ± 3.4 100 224.4 ± 7.7 239.5 ± 9.2 225.7 ± 8.7 150 213.1 ± 4.7 278.8 ± 4.2 246.8 ± 9.7 200 180.8 ± 4.5 304.1 ± 8.6 205.1 ± 11.7 300 154.3 ± 3.6 286.7 ± 6.9 164.5 ± 12.5

Example  2. Baikyeongryeong , Law and Cornerstone  Confirmation of Extraction Yield by Solvent of Fermented Extracts

The fermentation optimum medicinal plant added amount of snail mushroom hyphae mycelium through Example 1 was confirmed as 100g / L Baekryongyeong, 200g / L yulmu and 150g / L Choseok nap. The fermented extracts were filtered and then lyophilized to extract ethanol, hot water and purified water to find the optimal extraction conditions, and filtered to calculate the solid content to calculate the yield.

As a result, as shown in Table 5 below, all of the Baekbokryeong, Yulmu, and Choseok nap fermented with the Roe beetle mycelium mycelium were found to be the highest as 6.7%, 7.4%, and 7.1% in hot water extraction under 80 ° C.

Yield of Solvent Extracts from Fermented Roebuck Mushroom Mycelia menstruum yield(%) Fermented roe deer fungus mycelium Fermented Jelly of the Roe deer fungus mycelium Roe deer mushroom fungus mycelia
Fermentation Cornerstone ethanol 1.4 ± 0.2 2.4 ± 0.2 1.9 ± 0.2 Hot water (60 ℃) 4.2 ± 0.7 5.7 ± 0.3 4.9 ± 0.3 Hot water (80 ℃) 6.7 ± 0.2 7.4 ± 0.3 7.1 ± 0.2 Hot water (100 ℃) 5.2 ± 0.4 6.2 ± 0.2 6.2 ± 0.3 Purified water 2.6 ± 0.5 3.2 ± 0.2 3.1 ± 0.4

Example  3. Baikyeongryeong , Law and Cornerstone  Preparation of Mixture of Fermented Extracts and Confirmation of Cell Viability thereof

After culturing B16F10 mouse melanoma cells and dispensing 1 × 10 ⁵ cells / ml of each well in a 96-well plate and incubating them for 24 hours, Baekbokryeong prepared using the worm fungus mycelium, MTT (3- (4,5-dimethyldiazol-2-yl) -2 after 24 hours reaction was carried out by treating the mixture of Yulmu and Choseokjam fermentation extracts at concentrations of 10, 30, 50, 10 and 200 µg / ml, respectively. 10 μl of, 5-depenterazolium promid) reagent was added to form formazan for 4 hours. After the reaction was completed, the medium was removed, formazan was dissolved in dimethyl sulfoxide (DMSO), and the absorbance was measured at 540 nm using an ELISA reader, and the result was calculated as a ratio with respect to the untreated control value. As a result, as shown in FIG. 1, the mixtures of Baekbokryeong, Yulmu, and Choseok fermented extracts, which were prepared by mixing at various mixing ratios by using the Roe mung mushroom mycelium of the present invention, showed 100% cell viability at all treatment concentrations. It was confirmed that there was no cytotoxicity.

Example  4. Baikyeongryeong , Law and Cornerstone  Confirmation of melanin production inhibitory effect of mixture of fermented extract

Melanin production was measured using a modification of Hosei et al. (Hosei et al., 1985, Cancer Res., 45, 1474-1478). B16 / F10 melanocytes were cultured and incubated for 24 hours after treatment with a mixture of Baekbokryeong, Yulmu and Choseok fermented extracts of the present invention (Table 1) in 1 × 10 ⁵ cells / well in a 24 well plate. After 48 hours of incubation, each well was washed with PBS, 400 μl of 0.2N NaOH solution was added thereto, and dissolved at 60 ° C. for 1 hour. The absorbance was measured at 405 nm using an ELISA reader (Molecular Devices, USA). As a result, as shown in FIG. 2, the fermented extracts of each of Baekbokryeong, Yulmu, and Choseokjab prepared using the Roe-Mung mushroom mycelium and A, B, C, D, and E mixtures prepared according to various mixing ratios When comparing the melanogenesis inhibitory effect of 1), it was confirmed that the melanogenesis inhibitory effect is more significant than the single fermentation extract and A, B, C and E mixture when the 100 and 200 mg / ㎖ D mixture treatment.

Example  5. Baikyeongryeong , Law and Cornerstone  Elastase according to the mixture treatment of fermented extract and Collagenase  Confirmation of activity inhibitory effect

(1) inhibition of elastase activity

Elastase inhibitory activity was measured as follows. 50 μl of 0.4M Tris-HCl buffer (pH 8.6), the concentrations of Baek-Ryung, Yulmu and Choseok Ferment alone extracts and the mixture of 50 μL Tris-HCl buffer (pH 8.6) in 0.6 U / ml 50 μl of diluted enzyme (ex-porcine pancreas) was added and reacted at 37 ° C. for 30 minutes, and then 100 μl of substrate (methoxysuccinyl-L-Ala-L-Val-5-nitroanilide) was added. After the reaction, the absorbance was measured again at 410 nm. As a result, as shown in FIG. 3, the fermented extracts of each of Baekbokryeong, Yulmu, and Choseokjab prepared using the Roe mushroom fungus mycelium and A, B, C, D and E mixtures prepared according to various mixing ratios (Table When comparing the effects of inhibiting the elastase activity of 1), the effect of inhibiting the elastase activity than the single fermentation extract and the A, B, C and E mixtures when the D mixture treatment of 200 mg / ㎖ D statistically It was confirmed that there is a significant difference.

(2) Collagenase  Activity inhibition

Collagen is an ECM fibrillar molecule that constitutes the dermal dermal tissue and maintains the firmness of the skin. There are five types (I, II, III, IV and Ⅴ). It is due to the decrease in the synthesis capacity of or increased the resolution by collagenase. 0.25 ml and 0.25% of substrate solution in which 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg / mL) was dissolved in 0.1 M Tris-HCL buffer (pH 7.5) containing 4 mM CaCl ₂ . Baekbokryeong, Yulmu, and Choseokjam fermentation alone extracts diluted at 0.5% and 1% and 0.15ml of the mixed mixture were mixed and reacted for 20 minutes, and then 0.5ml of 6% citric acid was added to stop the reaction. 1.5 ml of ethyl acetate was added thereto, followed by centrifugation, and the supernatant was taken and absorbance was measured at 320 nm. Collagenase inhibitory activity was expressed as the absorbance decrease rate of the sample solution addition group and no addition group. As a result, as shown in FIG. 4, the fermented extracts of each of Baekbokryeong, Yulmu, and Choseokjab prepared using the Roe mushroom fungus mycelium and A, B, C, D, and E mixtures prepared according to various mixing ratios (Table When comparing the collagenase activity inhibitory effect of 1), when treated with a mixture of all concentrations of D except 10 mg / ㎖ concentration, the D mixture is a collagenase than a single extract and A, B, C and E mixture It was confirmed that the activity inhibitory effect is remarkable.

Claims (7)

Cosmetic composition for skin whitening and wrinkle improvement containing a mixture of Baekbokyeong fermentation extract, Yulmu fermentation extract and choseok nap fermentation extract as an active ingredient. According to claim 1, wherein the fermentation is a cosmetic composition for improving skin whitening and wrinkles, characterized in that the fermentation using the Roeden mushroom mycelium. The skin according to claim 2, wherein the mixture comprises 15 to 25 parts by weight of Baekbokyeong fermented extract, 25 to 35 parts by weight of yulmu fermented extract and 45 to 55 parts by weight of super salt fermented extract based on 100 parts by weight of the skin. Cosmetic composition for whitening and wrinkle improvement. The method of claim 1, wherein the Baekbokryeong fermentation extract, Yulmu fermentation extract and choseok nap fermentation extract are filtered by fermenting each of the fermentation of Baekbokryeong, Yulmu and Choseok nap, and freeze-dried the remaining residue, characterized in that it was prepared by hot water extraction Cosmetic composition for skin whitening and wrinkle improvement. According to claim 1, wherein the mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and choseok nap fermentation extract is
1) sterilizing at 110-130 ° C. for 10-20 minutes by adding 7-12 parts by weight of baekbokyeong, 17-22 parts by weight of yulmu and 12-17 parts by weight of zeolite to 100 parts by weight of medium, respectively;
2) inoculating each of the Roe mushroom fungus mycelium culture medium to each of the ingredients sterilized in step 1) and fermenting at 24 to 26 ℃ for 7 to 12 days;
3) filtering each fermentation product obtained in step 2) and lyophilizing the remaining residue;
4) obtaining each of the lyophilized fermented product of step 3) by hot water extraction at 75-85 ° C. to obtain Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok fermented fermentation extract; And
5) 15 to 25 parts by weight of Baekbokryeong fermentation extract, jujube fermentation extract and choseokmu fermentation extract obtained in step 4), 15 to 25 parts by weight of Baekbokyeong fermentation extract, 25 to 35 parts by weight of Yulmu fermentation extract and 45 to 55 A step of preparing a mixture by mixing the weight part of salt-free fermentation extract; Cosmetic composition for skin whitening and wrinkle improvement comprising the manufacturing. A skin external preparation for skin whitening and wrinkle improvement containing a mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient. Health functional food composition for improving skin whitening and wrinkles containing a mixture of Baekbokryeong fermentation extract, Yulmu fermentation extract and Choseok nap fermentation extract as an active ingredient.

Images