KR101950382B1 - Cosmetic Composition for Whitening of the Skin Comprising Fermented Extracts of Cassia tora, Phragmites communis, Sasa quelpaertensis, Anemarrhena asphodeloides and Gardenia jasminoides Ellis - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising a fermented solution obtained by culturing a mixture of Cassia tora, Phragmites australis, Sasa quelpaertensis, Anemarrhena asphodeloides, and Gardenia jasminoides with Saccharomyces cerevisiae or Lactobacillus. The fermented solution of Cassia tora, Phragmites australis, Sasa quelpaertensis, Anemarrhena asphodeloides, and Gardenia jasminoides of the present invention has excellent effects of inhibiting melanin production and tyrosinase activity, and excellent whitening effect.

Description

FIELD OF THE INVENTION The present invention relates to a cosmetic composition for skin whitening comprising a fermentation broth of a mixture of a catechol, a reed, a reed, a fermented juice,

The present invention relates to a cosmetic composition for skin whitening comprising a fermentation broth of a mixture of a cuttlefish, a reed, a liquorice of Jeju, a ginger, and a germanium mixture, and more particularly to a cosmetic composition for skin whitening comprising cultivating a mixture of a lucer, a reed, To a cosmetic composition for skin whitening comprising the fermentation broth of the mixture obtained.

Factors that determine skin color are basically skin color, such as tingling caused by spots, freckles and ultraviolet rays, or overall pigmentation, as well as differences in the distribution of acne and scarring and keratin Blood circulation, stress, and health. Among the above factors, the most important factor is the pigmentation.

Melanin, melanoid, carotene, hemoglobin, carotenoid, etc. are the coloring matters that affect the skin color, and various colors of skin, hair and eyes are determined by these pigments. The most important factor determining the color of the skin is melanin. The most important factors affecting the biosynthesis of melanin are ultraviolet light and hormone secretion in the body. Melanin absorbs or scatters ultraviolet light and plays a major role in preventing skin damage from ultraviolet rays. It does not have a specific maximum absorption wavelength and absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray.

The biosynthesis of melanin begins with the oxidation of tyrosine, a type of amino acid, into melanosomes of melanocytes and is converted to dihydroxyphenylalanine by tyrosinase, followed by a series of oxidation processes, starting with pheoelanin, It is formed of a polymer of eumelanin. This biosynthesis process is carried out in a special form of melanocyte in the brown intracellular organelle. The melanomas, including melanin granules, migrate from the nucleus to the tip of the dendritic process, and migrate into the cytoplasm by phagocytosis of keratinocytes. It accumulates around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to keratinocytes in the periphery are partially affected by hormones and ultraviolet rays, and are primarily affected by genetics. In addition, it is known that tyrosinase expression, melanin synthesis, and intracellular regulatory factors such as cytokine, metal ions such as copper, zinc and iron, and interferon, prostaglandin, and histamine are involved.

Meanwhile, in recent years, many cosmetics companies are making efforts to develop skin whitening ingredients, and the active ingredients obtained through fermentation are also applied to products.

For example, there is a case where a cosmetic composition is provided using a fermentation broth produced using Bacillus subtilis used in representative fermented foods such as Chungkukjang and natto. These fermented liquids have advantages of degradation of skin irritation due to decomposition of stimulating substances by microorganisms or conversion into other substances, and fermentation products by microorganisms are also widely used because they have a good effect on skin.

Korean Patent No. 10-0867202 discloses a whitening dermatological composition comprising an extract of Cercariae or emodine isolated therefrom. In Korean Patent Laid-Open No. 10-2007-0005049, . Korean Patent No. 10-0496850 discloses a whitening cosmetic composition containing a gardenia extract.

The present inventors have also developed a product using various natural products for this purpose, and have tried to improve skin whitening by using mixed extracts of Cucumber, Reed, Jeju Sage, Gamma, and Gardenia. Based on these existing data, the inventors of the present invention produced a lot of active ingredients by fermenting a mixture of Cucumber, Reed, Jeju Sage, Zygo, and Gardenia, and the skin whitening effect is much better than the mixed extract of Cereal, Reed, And the present invention has been completed.

The present invention relates to a cosmetic composition for skin whitening comprising a fermentation broth obtained by culturing a mixture of a cuttlefish, a reed, a bamboo basket, a ginger, and a gardenia with a yeast or a lactic acid bacterium.

Preferably, the yeast is Saccharomyces cerevisiae and the lactic acid bacterium is Bifido bacterium .

Preferably, the fermentation broth of the mixture is contained in an amount of 0.0001 to 30.0% (w / w) based on the total weight of the cosmetic composition.

Preferably, the fermentation broth of the mixture has an effect of inhibiting melanin production and inhibiting tyrosinase activity.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, Lt; / RTI >

The present invention relates to a cosmetic composition for skin whitening comprising a fermentation broth of a mixture of a cuttlefish, a reed, a bamboo basket, a ginger and a gardenia mixture. The fermentation broth of a combination of Cryptomeria japonica, Reed, Jeju Sauce, Gamma and Gardenia, which is an effective ingredient contained in the cosmetic composition of the present invention, is a fermentation broth obtained by cultivating a mixture of Cucumber, Reed, Jeju Sasa, Gamma and Gardenia with yeast or lactic acid bacteria, Production inhibitory effect and tyrosinase activity inhibitory effect, and exhibits excellent whitening effect.

In addition, the cosmetic composition containing the fermentation broth of a mixture of a cuttlefish, a reed, a cedar, a cedar, and a mixture of a gardenia of the present invention has a whitening effect compared to a cosmetic composition containing a solvent extract of a conventional mixture of a cuttlefish, reeds, .

The present invention provides a cosmetic composition for skin whitening comprising a fermentation broth of a mixture of a cuttlefish, a reed, a sourdough, a ginger and a gardenia mixture.

Cassia tora Linnaeus or Senna tora , which is used as an active ingredient in the composition of the present invention, is an annual plant that is planted near a house in North America. The stem is 1-2 m high and has no hair on the whole. Leaves are alternate phyllotaxis, small leaves 2-3 pairs. Leaves are obovate and 3-4 cm long. The tip of the leaf is dull and slightly pointed, the bottom is pointed or round, and the edge is flat. Flowers bloom in 1-2 axes on the axil and bloom in June-August. The fruit is ripened in the meadows, September-October, 10-15cm in length, roasted like a bow. It is cultivated all over Asia, and the seed is called a decapitator. The loser of the present invention uses seeds from various organs or parts (e.g., leaves, bark, branches, flowers, roots, fruits, stems, etc.) of the losers.

Reed's scientific name is Phragmites communis TRIN., rhizomes extend to the ground and roots fall from the nodes. The stem is green, hollow inside, and has hairs on the nodes. The height is about 2m and stands straight. Leaves are alternate, 2 to 3 cm in width, long and pointed. Flowers bloom in August and September with petal-free aquamarines. A large, elliptical cone inflorescence with numerous small flower buds hangs at the end of the stem, and at first it is brownish brown, and then it blooms with a light color. The fruit is a spirit and it has many tangents at the end of the seed, so it can blow away in the wind. The reeds of the present invention can be used in various organs or parts of reeds (e.g., leaves, bark, branches, flowers, roots, fruits, stems, etc.), preferably outposts or roots, Can be used.

The scientific name of Jeju Sori is Sasa quelpaertensis , and the leaves are long oval lanceolate with 2 ~ 3 pieces at the end of the branch. The height is about 1 to 2 m. The roots grow in the ground, bringing together newborn breeding and seed propagation through seeds. Flowers are known to bloom once every 5 to 6 years, but they are not constant, and when the flowers bloom, the top part dies. Sasa has both the properties of wood and the properties of grass. It is similar to a tree that has solid wood like a tree, but it is close to a perennial plant that stops growing in the first year and no longer grows in stem. It grows well in the shade, is strong against the cold, and likes the fertile and fertile soil. It has some ability to withstand pollution and salt damage, but it is weak in drying. The Jeju bran of the present invention can be applied to various organs or parts (e.g., leaves, bark, branches, flowers, roots, fruits, stems, etc.) of Jeju bran, preferably using outposts or roots, Outpost can be used.

His scientific name is Anemarrhena Asphodeloides BUNGE, a perennial herbaceous plant belonging to the family Zymo. It grows mainly in Seoheung, Hwanghae province. The rootstock is thick and spreads to the side, and the leaf grows at the end. Rhizome is used as a medicinal herb, it is effective for long-lasting fever in summer, and it has excellent antipyretic effect such as fever due to chronic or consuming disease. It is preferred that the buds of the present invention use roots among various organs or parts of buds (e.g., leaves, bark, branches, flowers, roots, fruits, stems, etc.).

Gardenia is the dried ripe fruit of the gardenia plant trees Rubiaceae (Gardenia jasminoides Ellis). Gardenia is planted in the central part of our country and in the greenhouse in the north. It is dried in the sun after the ripe fruit in autumn. The taste is good and the quality is cold. It acts on the heart, the menopause, the stomach, the liver, and the triceps. It lowers the heat, heals the chest tightly, removes the fever, and makes the urine come out well. It also stops the bleeding and sinks the bookkeeping and decodes. The gardenia of the present invention is used to dry the fruit of the gardenia, preferably the fruit of the gardenia.

1: 3: 1 ~ 3: 1 ~ 3: 1 ~ 3: 1 ~ 3, and preferably 1: 1: 1: 1: 1 Can be mixed in a weight ratio.

The fermentation broth of a combination of a lentil, a reed, a liquorice, a liquorice, a liquorice, a mixture of a liquorice, a liquorice, a mixture of a liquorice, a liquorice, and a mixture of a liquorice, a reed, a liquorice, a liquorice, And a mixture of germanium fermentation broth.

The yeast cells used for fermenting the sacrificial rye, reed, jeju sage, gilmo, and gardenia mixture of the present invention belong to eukaryotic microorganisms (organisms with nuclear and cytoplasmic membranes separated by nuclear membranes) and contain cell organelles such as cell wall, cell membrane, nucleus, mitochondria, It is equipped. Those with no distinction of sex mainly reproduce by budding. Among many isolated yeasts, there is no efficacy, proliferation by fission instead of budding, bud-fission method, injection by non-ascospore There are also diverse groups of yeasts, including those that form spores (ballistospores) or that do not exhibit spore formation. The size is usually 5-10 μm × 5-12 μm, 5-10 times the size of bacteria, and the optimum pH is 4.5-5.5 or even 1-10. Yeast surviving at pH 1 for 12 hours is stronger in acid than in bacteria, so if the pH of the medium is adjusted to about 3.5 to 3.8, bacterial contamination can be suppressed to some extent.

The yeast is slightly different depending on the species, but most of them grow well at 20 to 25 ℃. Normally, it is said that it will die at above 50 ℃, but it can be overcome to some extent by adding directly the growth factor which can not be synthesized above the temperature. In the case of Saccharomyces cerevisiae, it can be prevented by adding oleic acid and ergosterol to the medium at 40 ° C or higher. Yeast cells themselves are unicellular plants and contain large amounts of proteins and trace minerals. The yeast which is preferred for use in fermenting the Cereal, Reed, Jeju Sauce, Zygo, and Gardenia mixture of the present invention is Saccharomyces cerevisiae.

The lactic acid bacterium used for fermenting a mixture of a livestock of the present invention, a reed, a liquorice, a liquorice, and a gardenia mixture is a kind of microorganism beneficial to the human body. It decomposes carbohydrates such as glucose or lactose and produces an organic acid such as lactic acid to be. Lactic acid bacteria make lactic acid from sugar is called fermentation, and through this fermentation process, fermented food is produced. In the present invention, it may be a typical lactic acid bacteria of Lactobacillus, Streptococcus, bifidobacteria tumefaciens, Pocono stock flow, Phedi O Lactococcus, can be used for Lactococcus, preferably bifidobacteria tumefaciens (Bifidobacterium).

In the present invention, a fermentation broth of a cutter, a reed, a gypsum bamboo basket, a gypsum and a germanium mixture is prepared as follows:

a) finely granulating a mixture powder of a loser, a reed, a bamboo basket, a gum, and a gardenia using 100-500 mesh;

b) 1 to 50 g / L of a mixture powder of Celiaceae, Reed, Jeju Sauce, Gamma and Gardenia prepared in step a) is added to a conventional microorganism culture solution, and a yeast strain or a lactic acid bacterium is added in an amount of 10,000 to 100,000 cfu / L ;

c) culturing the culture of step b) at 30-37 ° C at pH 5-7 under aerobic or anaerobic conditions for about 5-10 days; And

d) filtering the culture of step c) and aging at low temperature for 5 to 10 days.

According to a preferred embodiment of the present invention, a fermentation broth of a cutlery, a reed, a bamboo basket, a ginger, and a germanium mixture used in the present invention was prepared as follows:

The yeast strain or the lactic acid bacteria were added at 50,000 cfu / L, and the mixture was cultured at 30 to 37 ° C in a pH of 5 To 7 for aerobic or anaerobic conditions for about 7 days in a fermenter. After culturing, a culture solution is obtained, and then the culture solution is filtered using 0.25 μM filter paper. The filtrate is further aged at low temperature for 10 days, filtered and concentrated to prepare a fermentation broth of the present invention, a reed, a reed, a gypsum and a germanium mixture.

In the present invention, a fermentation broth of a mixture of Cryptomeria japonica, Reed, Jeju Sauce, Gamma, and Gardenia inhibits tyrosinase activity and has a feature of improving skin whitening that inhibits melanin production.

In the present invention, the content of the fermentation broth of a combination of a lemon juice, a reed, a liquorice, a sugar beet and a gardenia is 0.0001 to 30.0% (w / w), more preferably 0.001 to 20% (w / w) . When the content of the fermentation broth of the mixture of Cereoptera, Reed, Jeju Sauce, Gamma and Gardenia is less than 0.0001% (w / w), it is impossible to expect a clear skin whitening improvement effect and the fermented product of Cereal, Reed, When the content is more than 30.0% (w / w), there is no significant increase in the effect according to the increase of the content, which causes skin irritation or problems in the stability of the formulation.

The components contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the fermentation broth of a mixture of a defibrillator, a reed, a reed, a bamboo basket, a ginger and a gardenia as an active ingredient. Examples thereof include antioxidants, stabilizers, , Customary adjuvants such as pigments and perfumes, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

Further, the present invention provides a cosmetic method for skin whitening cosmetic composition comprising a fermentation broth of a mixture of a lemon, a reed, a liquorice, a mixture of liquorice, and a mixture of a gardenia and a gardenia to a human skin to inhibit tyrosinase activity and melanin production to provide. The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. The cosmetic composition having an excellent skin whitening effect according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The cosmetic composition of the present invention, which comprises a fermentation broth of a mixture of a cuttlefish, a reed, a sourdough, a mixture of gypsum, gypsum and a mixture of gypsum, is applied to human skin to inhibit tyrosinase activity and melanin production and improve skin pigmentation So that skin whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1. Preparation of Yeast Fermentation Liquid of Cucumber, Reed, Jeju Sori, Gam and Gardenia Mixtures

After mixing 20g each of the seeds, seeds, reed, geranium, and gardenia, they were washed with purified water and dried to give a mixture of dried cuttlefish, reed, And then made fine using 300 mesh. This was added to a yeast culture solution (Potato Dextrose Broth, Akyumedia, USA) at 10 g / L. The yeast strains used for fermentation were Saccharomyces < RTI ID = 0.0 > cerevisiae) a was used, it was added at 50,000 cfu / L per culture. The cultures were maintained at 30 ° C and pH 5-7 for 7 days using a 5L fermenter. After culturing, the culture broth was centrifuged to remove the cultures first, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged at a low temperature warehouse at 4 ° C for about 10 days. The filtrate was filtered again using a 0.25 μM filter and used in the present invention.

Manufacturing example  2. Preparation of lactic acid fermentation broth of Cretaceae, Reed, Jeju Sasae, Gamma and Gardenia mixture

After mixing 20g each of the seeds, seeds, reed, geranium, and gardenia, they were washed with purified water and dried to give a mixture of dried cuttlefish, reed, And then made fine using 300 mesh. This was added to the culture solution of lactic acid bacteria to be 10 g / L. Glucose was added as a carbon source, and Dipotassium phosphate was added as a pH regulator. The lactic acid bacteria used here was Bifidobacterium longum , which was added at a concentration of 50,000 cfu / L per culture. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 5-7. After culturing, the culture broth was centrifuged to remove the cultures first, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged at a low temperature warehouse at 4 ° C for about 10 days. The filtrate was filtered again using a 0.25 μM filter and used in the present invention.

Comparative Manufacturing Example  1. Production of solvent extracts of Cereal, Reed, Jeju Sori, Gam and Gardenia mixture

20g of each of the seeds (seeds), reeds (outposts), Jeju boryeo (outposts), gilts (roots) and gardenia (fruits) were mixed together and then washed with purified water and dried to be powdered. Then, the mixture was added to 0.6 L of a 70% ethanol solution, and the mixture was heated and extracted with a reflux condenser equipped with a condenser for 4 hours. Then, the obtained extract was filtered with a 300-mesh filter cloth, filtered through filter paper No. 5, and then the solvent was removed using a vacuum concentrator and lyophilized to obtain 5.5 g of dry weight.

Experimental Example 1: Inhibition of tyrosinase activity of a fermentation broth of a mixture of Cryptomeria japonica, Reed, Jeju Sasa, Gamma and Gardenia

As a substrate, 40 μl of 1.5 mmol / l tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used. The fermented broths of Cucumber, Reed, Jeju Sasa, Zyma, and Gardenia mixture prepared in Preparation Example 1 and those prepared in Preparation Example 2 were dissolved in 0.05 M sodium phosphate buffer solution (pH 6.8 ) To prepare a sample solution for each concentration (0.5% (w / v), 1.0% (w / v)). To the solution was added 20 μl of tyrosinase (Sigma, 1500 U / ml), and the mixture was reacted at 37 ° C. for 15 minutes. Then, the mixture was reacted at 490 nm Absorbance was measured. As a comparative group, solvent extracts of Celiaceae, Reed, Jeju Sauce, Zygo and Gardenia mixture of Comparative Preparation Example 1 were used in place of the fermentation broth of a mixture of Celery, Reed, Jeju Sauce, Zygo and Gardenia. Activity inhibition of tyrosinase was calculated by the following formula (1). The experimental results are shown in Table 1 below.

Test substance concentration (%) Inhibition rate of tyrosinase activity (%) Preparation Example 1 0.5% (w / v) 48.6 Preparation Example 1 1.0% (w / v) 62.6 Preparation Example 2 0.5% (w / v) 53.9 Preparation Example 2 1.0% (w / v) 76.8 Comparative Preparation Example 1 0.5% (w / v) 31.6 Comparative Preparation Example 1 1.0% (w / v) 45.8

The results showed that when the fermented broth of Lactobacillus koreanum L. (Preparation Example 2) was treated with a defoliation agent, Reed, Jeju Sori, (Comparative Preparation Example 1), the fermentation broth of a mixture of Cryptomeria japonica, Reed, Jeju Sasa, Gamma and Gardenia inhibited the melanin production, And it was found that the pigment problem could be solved.

Experimental Example 2: Melanin biosynthesis inhibition experiment of fermentation broth of Celery, Reed, Jeju Sori, Zygo, and Gardenia

B-16 cells (mouse melanoma cells and Korean cell line bank) were inoculated on a 6-well plate at 1 × 10 ⁵ cells / well and cultured for 24 hours. The fermentation broth of Celery, Reed, Jeju Sage, Gamma and Gardenia was diluted with 0.05 M sodium phosphate buffer solution (pH 6.8) to a concentration of 0.5% (w / v) and 1.0% (w / v) After culturing and culturing for 3 to 4 days, the culture broth of each well was centrifuged. The separated cells were dissolved in 500 μl of 1N NaOH solution in dimethylsulfoxide (DMSO) and heated in a 90 ° C water bath for 10 minutes. The absorbance of this supernatant was measured at 490 nm. As a control group, a fermentation broth of a mixture of Cryptomeria japonica, Reed, Jeju Sasa, Gamma and Gardenia was used. The inhibition rate of melanin production was calculated according to the following formula (2), and the results are shown in Table 2.

Test substance concentration (%) Melanin production inhibition rate (%) Preparation Example 1 0.5% (w / v) 54.5 Preparation Example 1 1.0% (w / v) 79.1 Preparation Example 2 0.5% (w / v) 64.5 Preparation Example 2 1.0% (w / v) 83.9 Comparative Preparation Example 1 0.5% (w / v) 33.6 Comparative Preparation Example 1 1.0% (w / v) 57.7

When the fermented broth of Lactobacillus japonica was treated, the mixed extracts of Cereoptera, Reed, Jeju Sori, Gamma, and Gardenia were obtained from the above results. (Control group), the inhibitory effect of tyrosinase activity and the inhibitory effect on melanin biosynthesis were excellent, and the fermentation broth of a combination of Cryptomeria japonica, Reed, Jeju Sori, Gamma and Gardenia could solve the problem of skin pigmentation by suppressing melanin production And it was found. Based on the results of the above experimental examples, a cosmetic comprising a fermentation broth of a mixture of a cuttlefish, a reed, a bamboo sword, a ginger, and a germanium mixture of the present invention is prepared and presented. However, the composition of the present invention is not intended to be limited to the following formulations. The fermentation broth used in the following formulation example is selected from among the following: fermentation broth of fermented livestock, fermented livestock, fermented livestock, fermented livestock, fermented livestock, It is also acceptable.

Prescription Example 1: Softening Longevity

The following is an example of the formulation of the softening longevity in cosmetics containing a fermentation broth of a mixture of lucerum, reed, Jeju bran, gum, and gardenia.

ingredient content(%) Fermentation broth of mixture of liquor, reed, 0.5 glycerin 5.0 1,3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense, 0.01 Distilled water Balance Sum 100

Prescription example  2: Nourishing lotion

Examples of the prescription examples of the nutritional lotion among the cosmetics containing the fermented liquid of the mixture of the lemon juice, the reed, the sake bran, the ginger and the gardenia are as follows.

ingredient Content (unit:% by weight) Fermentation broth of mixture of liquor, reed, 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Methyl paraben 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription example  3: Nourishing cream

Examples of prescription examples of nutritional creams among cosmetics containing a fermentation broth of a mixture of Cucumber, Reed, Jeju Sauce, Kimchi, and Gardenia are as follows.

ingredient Content (unit:% by weight) Fermentation broth of mixture of liquor, reed, 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example 3: Whitening effect test of cosmetic composition

The whitening effect test of the cosmetic composition of the present invention was conducted on 40 Korean women of 20 to 40 years old, particularly skinned. Experimental products were prepared with 3% by weight of lactic acid fermentation broth, 3% by weight of fermented broth of Yeast, Reed, Jeju Sori, (Hereinafter referred to as " lactobacillus fermentation broth product ") were each prepared as described in Formulation Example 3. The control product was prepared according to the prescription of Prescription Example 3, except that the fermentation broth of a mixture of Celery, Reed, Jeju Sauce, Zygo and Gardenia was not contained. In addition, the comparative product was prepared according to the prescription of Prescription Example 3 so that the solvent extract of Crassosaurus, Reed, Jeju Sauce, Zygo and Gardenia mixture was contained in an amount of 3 wt% instead of the fermentation broth.

In the experiment, first, the control product and the mixture yeast fermentation product were used on both sides of 20 subjects 'faces, and 20 subjects used the comparative product and the lactic acid fermentation product on both sides of 20 subjects' faces. Minolta CR 300 was used for the measurement and it was measured for 8 weeks. The experimental results were compared with the L value taken at 8 weeks and the L value taken at 0 week. The results are shown in Table 6 below.

division Contrast product Compare Products Mixed yeast fermented milk products Mixed lactic acid fermented broth product DELTA L value 0.124 0.234 0.455 0.467

As shown in Table 6, the products containing the fermentation broth of the combination of Cereal, Reed, Jeju Sauce, Zygote and Gardenia showed higher whitening effect than the control products. In addition, it showed much higher whitening effect than the comparative product containing the solvent extract of Cucumber, Reed, Jeju Sauce, Zygo, and Gardenia mixture. In other words, the whitening effect is increased by fermenting the extracts of Cryptomeria japonica, Reed, Jeju Sasa, Zygomoe, and Gardenia which are active in whitening. Therefore, it can be seen that the cosmetic composition of the present invention has an excellent whitening effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

The mixture weight ratio is 1: 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3, and the mixture weight ratio is 1: , The yeast is Saccharomyces cerevisiae , and the lactic acid bacterium is Bifido bacterium . The cosmetic composition for skin whitening according to claim 1, wherein the fermentation broth is contained in an amount of 0.0001 to 30.0% (w / w) based on the total weight of the cosmetic composition. The cosmetic composition for skin whitening according to claim 1, wherein the fermentation broth has an effect of inhibiting melanin production and inhibiting tyrosinase activity. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Lt; RTI ID = 0.0 > cosmetic < / RTI > composition for skin whitening. delete