KR20130001842A - Cosmetic composition and makeup method for the skin whitening comprising the styrax obassia extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Styrax obassia extract is provided to suppress melagnogenesis and tyrosinase activity and to resolve pigmentation problem. CONSTITUTION: A cosmetic composition for skin whitening contains 0.00001-25.0 wt% of Styrax obassia extract. The extract is prepared using water, anhydrous or hydrous low carbon number alcohol of C1-C4, propylene glycol, butylene glycol, glycerine, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, hexane, or a mixture thereof. The extract is prepared using microorganisms such as Lactobacillus, Bipidobacterium, Streptococcus, Leuconostoc, Pediococcus, Lactococcus, or a mixture thereof. The cosmetic composition is manufactured in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a powder, a soap, a surfactant-containing cleansing, oil, a powder foundation, an emulsion foundation, a wax foundation, or a spray.

Description

Cosmetic composition and cosmetic method for skin whitening including a camellia extract {Scotch composition and makeup method for the Skin Whitening Comprising the Styrax obassia Extracts}

The present invention relates to a cosmetic composition comprising a camellia extract, and more particularly, a camellia extract obtained by treating the camellia in various ways such as solvent extraction, ultrasonic extraction, supercritical extraction, fermentation or foaming. It relates to a cosmetic composition for skin whitening and a cosmetic method comprising the extract camellia containing.

Human skin not only functions to protect our bodies, but also has a deep connection with skin beauty. In particular, the color of the skin is affected by the skin pigments and occupies the most important position in skin care, which has been the subject of much research in the cosmetic industry. The pigments that affect skin color include melanin, carotene, and hemoglobin, the most important of which is melanin.

In addition, the human tarosinase enzyme is responsible for the production of melanin, which represents the human skin color.

Melanin plays an important role in preventing or damaging the skin from ultraviolet rays by absorbing or scattering ultraviolet rays, and absorbs light in all areas without a specific maximum absorption wavelength. In addition, the function of removing active oxygen species is excellent, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in melanin structure, and melanin itself shows the properties of free radicals. Pray.

 Melanin, which plays such an important role, may cause skin pigmentation if it is overproduced in the skin by external stimuli, which may adversely affect skin beauty.

On the other hand, the skin whitening cosmetic composition comprising the extract camellia has not been found in the literature, except that the cosmetic composition using Styrax japonica belonging to the same family as the camellia was known as a Korean Patent Registration No. 10-526888 It contains lignin glucosite as a main ingredient, simply to prevent skin aging, and could not provide a whitening effect.

The present inventors have focused on such a phenomenon and have developed a material that suppresses melanin overproduction using natural products, and the cosmetic composition using Styrax obassia extract typically has tyrosinase activity inhibitory effect and inhibits melanin biosynthesis. The present invention was completed by confirming that skin hyperpigmentation can be prevented and skin whitening can be effectively improved.

The extract of camellia in the present invention can be extracted by a method selected from a solvent extraction method, an ultrasonic extraction method, a supercritical extraction method, a fermentation method or a foaming method.

That is, an object of the present invention is to provide a cosmetic composition for skin whitening comprising a camellia extract.

Still another object of the present invention is to provide a cosmetic method comprising applying a cosmetic composition for whitening camellia extract to the skin to inhibit tyrosinase activity and inhibit melanin biosynthesis to exhibit skin whitening effect. will be.

In the present invention, in order to provide a whitening cosmetic composition using a camellia camellia extract was prepared by using a variety of extraction methods and applied to the product. To this end, the present inventors prepared the extract of the camellia by using a common solvent, including the extract of the camellia obtained by using fermentation, ultrasonic, supercritical, foaming method as an active ingredient, the object of the present invention as described above Silver is achieved by a cosmetic composition for skin whitening comprising a camellia extract, characterized in that it contains the extract as an active ingredient.

Preferably, the camellia extract is characterized in that it contains 0.00001 to 25.0% by weight based on the total weight of the cosmetic composition.

Also, preferably, the extract of Camellia camellia is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, Solvent extraction using an extraction solvent selected from the group consisting of dichloromethane, hexane and mixtures thereof; Ultrasonic extraction method; Supercritical extraction method; zymotechnics; Or it extracts using the foaming method.

In addition, the fermentation method is characterized in that the extract obtained by culturing Saccharomyces cerevisiae as a yeast camellia as a yeast.

Also preferably, the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It is characterized by having a formulation selected from the group.

Another object of the present invention is achieved by a cosmetic method characterized by having a tyrosinase activity inhibition, melanin biosynthesis and whitening effect by applying to the skin using the cosmetic composition described above.

The present invention suppresses the skin pigmentation caused by melanin overproduction by adding the extract camellia extract to the cosmetic, it is possible to obtain a cosmetic composition with a very good whitening effect.

More specifically, the cosmetic composition for skin whitening containing the camellia extract according to the present invention has a tyrosinase activity inhibitory effect (Experimental Example 1), melanin biosynthesis inhibitory effect (Experimental Example 2) and a whitening effect (Experimental Example 3) Is excellent.

Therefore, in the case of the cosmetic containing the extract camellia of the present invention, it has an excellent whitening effect, when developing it as a cosmetic material can be expected excellent pigmentation improvement effect (skin whitening effect),

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising a camellia extract.

The camellia ( Styrax obassia ) used as an active ingredient in the composition of the present invention has a deciduous broad-leafed arborescent form as a deadwood plant. The features are as follows.

It grows about 10m high, and the bark is dark blackish gray and smooth. Winter snow comes from the bottom of petioles. Leaves are alternate, narrow oval or round with jagged teeth on top, often divided into three. The green surface has hairs on the veins and the back is white. Petiole 5 ~ 20mm. In May-June, about 20 white flowers run downward from the end of new branches. Corollas are divided into four ends, and calyxes are divided into 5-9 parts. There are several stamens and one pistil, and there is no hair on the operating table and the pistil. Fruits are nucleus, ovate or oval, with hairs. Ripens in September, with uneven crusts and brown seeds. It is a deciduous broad-leaved arborescent growing in the forests of mountainous regions of 100 ~ 1,800m nationwide. It is native to Korea and is distributed in China, Japan, and Manchuria.

On the other hand, the term "p camellia" in the present specification means a variety of parts "branch, root, leaves, flowers" of the camellia, and completed the present invention with the flower part as the main material.

     The camellia extract of the present invention is prepared according to conventional methods known in the art, i.e., under conditions of conventional temperature and pressure, using conventional solvents (e.g., water, anhydrous or hydrous lower alcohols). (E.g., methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, butyl acetate, 1,3-butylene glycol and mixtures thereof, obtained using one or more extraction solvents selected from the group consisting of It is preferably obtained using ethanol, 70% (v / v) aqueous ethanol or water, most preferably obtained using 70% ethanol aqueous solution.

On the other hand, it is apparent to those skilled in the art that the camellia extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

According to a preferred embodiment, the method of preparing the camellia extract of the present invention is as follows. First, washed the camellia flowers with purified water, dried and crushed into small pieces so that the particle size is less than 300 mesh, and then 1 to 10 times the dry weight of water, 1 to 4 carbon atoms anhydrous or lower alcohol , Acetone, ethyl acetate, butyl acetate or 1,3 butylene glycol are added. After the cooling condenser is installed to prevent the active ingredient is evaporated in the state of extraction for 3 to 20 hours by heating at 40 to 100 ℃, or extracted for 1 to 15 days at 4 to 40 ℃ and completely dried by a rotary vacuum evaporator to prepare . In this case, 1,3 butylene glycol is difficult to dry by using a rotary vacuum evaporator, so the water content is dried in a hot air dryer at 30 ℃ ~ 60 ℃ to extract 1% (w / v) directly after extraction under the above conditions Is used for the cosmetic of the present invention.

Meanwhile, the camellia extract of the present invention includes not only the extract by the above-described extraction method, but also an extract by supercritical extraction or ultrasonic extraction by reduced pressure and high temperature with carbon dioxide. The decompression by carbon dioxide and the supercritical extraction by high temperature mean supercritical fluid extraction. Generally, the supercritical fluid is a liquid having a gas when the gas reaches a critical point under high temperature and high pressure. It is gaseous, chemically similar in polarity to nonpolar solvents, and supercritical fluids are used for the extraction of fat-soluble substances ( J. Chromatogr . A. 1998; 479: 200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gaseous properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a co-solvent is added to carbon dioxide. Such cosolvents are selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane diethyl ether and mixtures thereof.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

As an example of a method for producing a camellia extract by the supercritical extraction method, the camellia flowers are washed with purified water, dried and broken into small pieces of 300 mesh or less, and then the sample is placed in a supercritical extraction vessel and the liquid chromatography pump is By using pure carbon dioxide in supercritical state at a suitable temperature and pressure, the camellia extract is received in a test tube.

In supercritical extraction, carbon dioxide is at a temperature of 35 to 100 ° C., preferably 50 to 70 ° C., more preferably about 60 ° C., 100 to 500 atmospheres, preferably 200 to 400 atmospheres, more preferably about 300 Supercritical is recommended under pressure.

According to the ultrasonic extraction method of the camellia of the present invention, the camellia or the crushed camellia is placed in an extraction solvent selected from chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether, and mixtures thereof, followed by an ultrasonic extractor (supernonic , The United States) to extract for about 1 to 6 hours at 30 ℃ with an intensity of 25KHz, The ultrasonic extraction time may vary depending on the amount of sample to be extracted.

The extraction solvent that can be used in the ultrasonic extraction method is selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof.

The extracted sample is vacuum filtered to recover the filtrate, and then removed by a vacuum evaporator, the extract can be obtained through a conventional extract preparation method of freeze drying.

The camellia extract of the present invention also includes an extract that has undergone conventional purification and / or fermentation. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also interpreted to be included in the extract of Camellia of the present invention.

In addition, the fermentation product obtained by fermentation for 1 to 7 days while maintaining a pH of 5 to 7 under temperature conditions of 20 to 40 ℃ as a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria and mixtures thereof as a fermentation process. It is interpreted to be included in the extract of camellia.

The yeast used for fermenting the camellia of the present invention belongs to eukaryotic microorganisms (organisms in which the nucleus and cytoplasm are divided by the nuclear membrane), and has cell organelles such as cell walls, cell membranes, nuclei, particulates, and granules. Those without sex distinction breed mainly by budding, among which many of the isolated yeasts have no fermentation capacity, multiply by fission or bud-fission instead of budding, and not ascospore. There are also various groups of yeasts, such as forming spores or not forming spores. The size is usually 5 × 5 ^-12 ~ 10 × 5 ^-12 ㎛, 5-10 times the size of bacteria, and the optimal pH survives at 4.5-5.5 but 1-10. Yeasts that survive 12 hours at pH 1 are more acid resistant than bacteria, so adjusting the pH of the medium to about 3.5 to 3.8 can reduce bacterial contamination.

Yeast is slightly different depending on the species, but most grow well at 20 ~ 25 ℃. Usually, it is killed above 50 ℃, but it can be overcome to some extent by adding the growth factor which is not synthesized above the temperature directly to the medium. Saccharomyces cerevisiae ( Saccharomyces cerevisiae) is killed at 40 ℃ or more, but can be prevented by adding oleic acid (oleic acid) and ergosterol to the medium. Yeast cells themselves are single-celled plants that contain large amounts of protein and trace minerals. A preferred yeast for use in fermenting the camellia of the present invention is Saccharomyces cerevisiae.

In addition, lactic acid bacteria that can be used to ferment the camellia in the present invention is a kind of microorganisms very beneficial to the human body to break down carbohydrates such as glucose or lactose to produce organic acids such as lactic acid (lactic acid) or acetic acid. Generally, lactic acid bacteria make lactic acid from sugar is called fermentation, and fermented food is made through this fermentation process. Lactic acid bacteria that can be used in the present invention preferably include Lactobacillus, Streptococcus, Bifido bacterium, Leukonostock, Pediococcus, Lactococcus and mixtures thereof, most preferably Lactobacillus . Among the Lactobacillus, the most preferred lactic acid bacteria for use in fermenting the camellia of the present invention is Lactobacillus plantarum .

In addition, Bacillus bacteria can be used as the fermentation bacteria used to ferment the camellia in the present invention, Bacillus is spore-forming bacteria, inhabits in soil or water and is mostly associated with organic matter decomposition. Bacillus bacteria that can be used in the present invention preferably include Bacillus subtilis, Bacillus pumilis, Bacillus rikenimomis, Bacillus megaterium, Bacillus polypermanticus, Bacillus mesentericus and mixtures thereof Most preferably, Bacillus subtilis is known as Bacillus subtilis , which anaerobicly metabolizes various sugars such as glucose, starch, etc., and is used for fermented foods such as Meju and Cheonggukjang. It is a microorganism that breaks down organic matter.

Moreover, the camellia extract of this invention also contains the extract obtained using the preparation method. Foaming agent is a pharmaceutical technology that changes the original properties of the medicine by processing the medicine based on the herbal theory. Mainly refers to the process of steaming, boiling, roasting, roasting or roasting or a process in which these are mixed. The treatment conditions are 60-100 ° C. for 30 minutes to 6 hours when boiled, 120 to 180 ° C. for 10 minutes to 2 hours for steaming, and 80-100 ° C. for 10 minutes to 2 hours for baking. In the present invention, the dried camellia was steamed and used to prepare an extract using the same.

In the present invention, the dried camellia was steamed and used to prepare an extract using the same. After extracting the dried camellia by steaming and drying it, it can be extracted not only by the solvent extraction method, but also by the extraction method by the supercritical extraction method or the ultrasonic extraction method under reduced pressure with carbon dioxide and high temperature, 70% in the embodiment of the present invention. Extraction using ethanol was used for the experiment.

The camellia extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

 According to a more preferred embodiment of the present invention, the extract camellia of the present invention is excellent in tyrosinase activity inhibitory effect and melanin biosynthesis inhibitory effect.

     In addition, according to a preferred embodiment of the present invention, the content of the camellia extract of the present invention is 0.00001 to 25.0% by weight, preferably 0.0001 to 20.0% by weight relative to the total weight of the cosmetic composition. At this time, when the total weight of the camellia extract is less than 0.00001% by weight, the effect is less likely to appear, when it exceeds 25.0% by weight is likely to cause irritation to the skin and may have a significant effect on the stabilization of the formulation.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract of camellia as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, And carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations, and sprays. More specifically, it may be prepared in the form of a flexible lotion (skin), nutrition lotion (milk lotion), nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

In addition, the present invention provides a cosmetic method comprising the skin whitening effect by using a cosmetic composition for skin whitening containing the extract camellia inhibits melanin biosynthesis, inhibits tyrosinase activity.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition excellent in the skin whitening effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing or a surfactant-free cleansing formulation, it may be wiped off after application to the skin, or may be washed off with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Performing a cosmetic method comprising applying a cosmetic composition comprising the extract of the camellia of the present invention to human skin, inhibits tyrosinase activity and melanin biosynthesis, and improves skin pigmentation to obtain a skin whitening effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Camellia  Extract Preparation I

     To 200 g of dried camellia flowers, 1,000 ml of an aqueous 70% ethanol solution having a ratio of water and ethanol 7: 3 was added thereto, and the mixture was leached at room temperature for 4 days. It was filtered and the filtrate was concentrated in vacuo to give a dry extract, the extraction yield was 15.8g / 200g. The dry extract was dissolved in 50% 1,3-butylene glycol solution to a concentration of 1% (w / v) and filtered and then subjected to the experiment of the present invention.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Except for using the extraction solvent of Table 1 was extracted in the same manner as in Example 1 to obtain a camellia extract.

Camellia  Preparation of extract II

Example 2 to Example 16 was extracted in the same manner as in Example 1 except that the extraction solvent of Table 1 was used to obtain a camellia extract. In addition, Examples 15 to 16 are conventional supercritical extraction method (extracting cosolvent using olive oil in supercritical state under a pressure of about 300 atm at a temperature of about 60 ° C.), ultrasonic waves (superonic ultrasonic apparatus) To extract the camellia extract through the extraction method using a method of extracting for about 2 hours at 30 ℃ with an intensity of 25KHz. However, in Example 15, olive oil was used as a cosolvent, and in liquid form, purified water was used in Example 16.

Extraction yield of camellia extract division Extraction solvent From 1 kg of camellia
Weight of extract obtained in g Example 2 Purified water 26.4 Example 3 25% ethanol aqueous solution 24.2 Example 4 50% aqueous ethanol solution 26.4 Example 5 100% ethanol 26.7 Example 6 80% methanol aqueous solution 24.5 Example 7 100% methanol 23.8 Example 8 100% acetone 22.7 Example 9 100% diethyl ether 21.4 Example 10 100% Hexane 18.5 Example 11 100% normal-propanol 20.3 Example 12 100% isopropanol 20.6 Example 13 100% normal-butanol 25.5 Example 14 100% ethyl acetate 19.7 Example 15 Supercritical extraction 182.5 Example 16 Ultrasonic extraction 28.3

Camellia  Preparation of Yeast Fermented Extract

The dried camellia was washed with purified water and dried to make fine powder using 300 mesh. This was added to yeast culture medium (Potato Dextrose Broth, Accumedia, United States) to 10 g / L.

Saccharomyces cerevisiae was used as a yeast strain for fermentation, and was added at 50,000 cfu / L per culture. Cultivation was carried out using 5L fermenter for 7 days, maintained at 30 ℃, pH 5-7. Centrifugation of the cultured camellia yeast fermentation was centrifuged to remove the cultures first, and then filtered through 0.25 μM filter paper. The filtrate thus obtained was aged again for about 10 days in a low temperature warehouse at 4 ° C., and then the filtrate was filtered again using a filter of 0.25 μM, and used for the present invention. This is called. The camellia yeast fermented extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Camellia  Preparation of Lactic Acid Bacteria Fermented Extract

The dried camellia was washed with purified water and dried to make fine powder using 300 mesh. Add 10 g / L of lactic acid bacteria culture medium (Lactobacillus MRS Broth, Difco, USA) and mix After sterilization at 121 ℃ was inoculated with lactic acid bacteria.

Lactobacillus used for fermentation was used Lactobacillus plantarum ( Lactobacillus plantarum ), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 30 ℃, pH 5-7. Centrifugation of the cultured camellia fermentation broth was centrifuged to remove primary cultures, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged again in a low temperature warehouse at 4 ° C. for about 10 days, and the filtrate was filtered again using a filter of 0.25 μM, and used for the present invention. It is called. The camellia lactobacillus fermented extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Camellia Bacillus  Preparation of Fermented Extract

The dried camellia was washed with purified water and dried to make fine powder using 300 mesh. This was added to the Bacillus culture medium at 10 g / L.

Bacillus strains used for fermentation were used Bacillus subtilis ( Bacillus subtilis ), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 30 ℃, pH 5-7. Centrifugation of the cultured camellia fermentation broth was centrifuged to remove primary cultures, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged again in a cold warehouse at 4 ° C. for about 10 days, and then the filtrate was filtered again using a filter of 0.25 μM, and used for the present invention. This is called. The camellia Bacillus fermented extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Camellia A preparation method  extraction

Boiled, steamed, roasted, roasted or baked, or a process in which these were mixed to obtain the extract of the method of sapling camellia of the present invention was carried out as follows. In other words, after washing with purified water and dried dried camellia branches to make fine using 300 mesh, steaming was carried out under a condition of temperature 100 ℃, 1 hour and then dried. The extract of the prepared camellia was thus extracted using a 70% ethanol solution.

Experimental Example  One: Camellia  Inhibition of Tyrosinase Activity of Extracts

40 μl of 1.5 mmol / L tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used as the substrate. The sample camellia extracts prepared in Preparation Examples 1 to 18 were each diluted with 0.05 M sodium phosphate buffer solution (pH 6.8) to have a concentration of 1.0% (v / v), respectively. 240 μl of the sample solution for each concentration was added to 40 μl of tyrosine, and 20 μl of tyrosinase (Sigma, 1500 U / ml) was added to the solution, followed by reaction at 37 ° C. for 15 minutes, followed by 490 nm. Absorbance was measured. In this case, 1.0% of arbutin, a substance known to have an inhibitory effect on tyrosinase activity, was used instead of the extract of camellia as a positive control group. When the absorbance was measured, sodium phosphate buffer solution (pH 6.8) was used instead of the camellia extract.

Inhibition rate (%) of tyrosinase activity was calculated by Equation 1 below, and the experimental results are shown in Table 2 below.

(= Tyrosinase activity inhibition rate (%))

Camellia  Inhibitory Effects of Extracts on Tyrosinase Activity Test substance Tyrosinase activity inhibition rate (%) Example 1 (70% ethanol aqueous solution extract of camellia) 76.8 Example 15 (Supercritical Extract of Camellia Camellia) 72.4 Example 16 (Sonic Camellia Extract) 78.2 Example 17 (Camellia yeast fermentation extract) 77.6 Example 18 (Camellia lactobacillus fermentation extract) 76.8 Example 19 (Camellia Bacillus Fermented Extract) 75.8 Example 20 (extract method extract of camellia) 80.5 Positive control group (arbutin) 69.2

Through the above results, the camellia extract (Examples 1, 15-20) had a superior or similar inhibitory effect on tyrosinase activity compared to the arbutin, a positive control group, from which the camellia extract of the present invention exhibited tyrosinase activity. It can be seen that by inhibiting the skin pigment problem can be solved.

Experimental Example  2 : Camellia  Melanin Biosynthesis Inhibition Experiment

B-16 cells (rat melanoma cells, Korea Cell Line Bank) were seeded in 6-well plates to 1 × 10 ⁵ cells / well and incubated for 24 hours. Each well was treated with 1% (v / v) of the camellia extract, and then cultured for 3-4 days, and the culture solution of each well was centrifuged. The isolated cells were lysed with 500 μl of 1N NaOH solution dissolved in dimethyl sulfoxide (DMSO) and heated in a 90 ° C. water bath for 10 minutes. This supernatant was measured for absorbance at 490 nm. As a positive control, 1.0% of arbutin, which is known to have melanin biosynthesis inhibitory effect, was used instead of the extract of camellia. As a control, 1N NaOH solution dissolved in dimethyl sulfoxide (DMSO) was used instead of the camellia extract. Melanin biosynthesis inhibition rate (%) was calculated according to the following equation (2), the results are shown in Table 3.

 (= Melanin biosynthesis inhibition rate (%))

Inhibitory Effects of Camellia Camellia Extract on Melanin Biosynthesis Test substance Melanin biosynthesis inhibition rate (%) Example 1 (70% ethanol extract of camellia) 76.5 Example 15 (Supercritical Extract of Camellia Camellia) 78.8 Example 16 (Sonic Camellia Extract) 74.3 Example 17 (Camellia yeast fermentation extract) 72.9 Example 18 (Camellia lactobacillus fermentation extract) 70.6 Example 19 (Camellia Bacillus Fermented Extract) 76.6 Example 20 (extract method extract of camellia) 79.8 Positive control group (arbutin) 69.9

Through the above results, the treatment of the camellia extract was superior to the melanin biosynthesis effect compared to the arbutin positive control group, and from this it was found that the camellia extract can solve the skin pigment problem by inhibiting melanin biosynthesis. .

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition comprising the extract camellia of the present invention. However, the composition of the present invention is not intended to be limited to the following prescription examples.

Prescription example  1: softening longevity

Prescription examples of soft cosmetics in cosmetics containing the extract camellia as follows.

ingredient content(%) Japanese camellia extract 0.5 glycerin 5.0 1.3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense, 0.01 Distilled water Balance Sum 100

Prescription example  2: nutrition cosmetics

 Prescription examples of nutrient cosmetics in cosmetics containing extract of camellia are as follows.

ingredient Content (unit: weight%) Japanese camellia extract 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1.3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 Methyl paraben 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription example  3: Nourishing cream

 Prescription example of nutrition cream among cosmetics containing extract of camellia is as follows.

ingredient Content (unit: weight%) Japanese camellia extract 3.0 Lipophilic monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example  3: Camellia  Containing extract Cosmetic  Whitening effect experiment

A whitening effect test of the cosmetic composition of the present invention was carried out on 20 Korean women aged 20 to 40 with dark skin.

As a test group, a cosmetic containing 3.0% of camellia fermented extract of Example 17 was used, and the composition thereof is shown in Table 7. As a control, experiments were carried out using cosmetics containing the same amount of purified water and arbutin, a substance known to have a whitening effect as a positive control group, as a control, instead of the extract of the camellia fermentation.

ingredient Content (unit: weight%) Japanese camellia extract 3.0 Lipophilic monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

In the experiment, 20 subjects of 20 cosmetics containing a control and a camellia fermented extract were used on both sides of each face. Minolta CR 300 was used as a measuring instrument and was measured for 8 weeks. Experimental results were compared to L value taken at 8 weeks of skin lightness value (Lightness) and L value taken at 0 weeks before the start of the experiment and the results are shown in Table 8 below.

division Control group Japanese camellia extract Positive control group (arbutin) △ L value 0.168 0.602 (0.413)

As shown in Table 8, the △ L value of the cosmetic containing the extract camellia was measured higher than the control cosmetics, it was shown that the whitening effect of the cosmetic containing the extract camellia fermentation of the present invention. In addition, it can be seen that the cosmetic composition including the camellia fermented extract of the present invention has a very good whitening effect compared to the cosmetic containing the arbutin positive control group.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

Cosmetic composition for skin whitening comprising a camellia extract, characterized in that it contains an extract of parsley camellia as an active ingredient. According to claim 1, The camellia extract is a cosmetic composition for skin whitening comprising a camellia extract, characterized in that it contains 0.00001 to 25.0% by weight relative to the total weight of the cosmetic composition. The method of claim 1 or 2, wherein the extract camellia camellia extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, di Any one selected from solvent extraction using at least one extraction solvent selected from the group consisting of ethyl ether, dichloromethane, hexane, and mixtures thereof, ultrasonic extraction, supercritical extraction, fermentation using microorganisms or extraction by foaming A cosmetic composition for skin whitening comprising a camellia extract, which is obtained by a method. 4. The cosmetic composition for skin whitening of claim 3, wherein the microorganism is an extract obtained by culturing with Saccharomyces cerevisiae as a yeast. The method of claim 3, wherein the microorganism is a lactic acid bacterium, obtained by culturing with a microorganism selected from the group consisting of Lactobacillus, Bifidobacterium, Streptococcus, Leukonostock, Pediococcus, Lactococcus and mixtures thereof. Cosmetic composition for skin whitening comprising an extract of camellia, characterized in that the extract. The method of claim 3, wherein the microorganism is Bacillus sp. ( Bacillus sp. ), Bacillus subtilis, Bacillus pumilis, Bacillus liqueniformis, Bacillus megaterium, Bacillus polyfermentus, Bacillus mesentericus And cosmetic composition for skin whitening comprising a camellia extract, characterized in that selected from the group consisting of these. The method of claim 1 or 2, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and Cosmetic composition for skin whitening comprising a camellia extract, characterized in that it has a formulation selected from the group consisting of a spray. A cosmetic method comprising: applying to the skin using the cosmetic composition according to any one of claims 1 to 7, having tyrosinase inhibitory activity, melanin biosynthesis inhibition and whitening effect.