KR20180041351A - Cosmetic Composition for Whitening of the Skin Comprising the Extract of Fermented Tillandsia usneoides - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic composition comprising a fermented bean tallow extract and a cosmetic method, which is obtained by culturing beard tillandiae with yeast or lactic acid bacteria. According to the invention The hydrolyzate of hydrolyzate of T. rubica is excellent in the effect of inhibiting melanin formation and inhibiting tyrosinase activity, and the cosmetic composition for skin whitening comprising the hydrolyzate of hydrolyzate of T. rubica according to the present invention is excellent in whitening effect.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin whitening comprising a fermented bean tilandia fermentation liquid,

The present invention relates to a skin whitening cosmetic composition comprising a fermented bean curd tile extract, and more particularly to a cosmetic composition for skin whitening comprising a fermented bean curd tylans obtained by culturing a bean curd tylanshia with yeast or lactic acid bacteria .

Factors that determine skin color are basically skin color, such as tingling caused by spots, freckles and ultraviolet rays, or overall pigmentation, as well as differences in the distribution of acne and scarring and keratin Blood circulation, stress, and health. Among the above factors, the most important factor is the pigmentation.

Melanin, melanoid, carotene, hemoglobin, carotenoid, etc. are the coloring matters that affect the skin color, and various colors of skin, hair and eyes are determined by these pigments. The most important factor determining the color of the skin is melanin. The most important factors affecting the biosynthesis of melanin are ultraviolet light and hormone secretion in the body. Melanin absorbs or scatters ultraviolet light and plays a major role in preventing skin damage from ultraviolet rays. It does not have a specific maximum absorption wavelength and absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray.

The biosynthesis of melanin begins with the oxidation of tyrosine, a type of amino acid, into melanosomes of melanocytes and is converted to dihydroxyphenylalanine by tyrosinase, followed by a series of oxidation processes, starting with pheoelanin, It is formed of a polymer of eumelanin. This biosynthesis process is carried out in a special form of melanocyte in the brown intracellular organelle. The melanomas, including melanin granules, migrate from the nucleus to the tip of the dendritic process, and migrate into the cytoplasm by phagocytosis of keratinocytes. It accumulates around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to keratinocytes in the periphery are partially affected by hormones and ultraviolet rays, and are primarily affected by genetics. In addition, it is known that tyrosinase expression, melanin synthesis, and intracellular regulatory factors such as cytokine, metal ions such as copper, zinc and iron, and interferon, prostaglandin, and histamine are involved.

Meanwhile, in recent years, many cosmetics companies are making efforts to develop skin whitening ingredients, and the active ingredients obtained through fermentation are also applied to products.

For example, there is a case where a cosmetic composition is provided using a fermentation broth produced using a fermented product of Bacillus subtilis, which is used in representative fermented foods such as chonggukjang and natto. These fermented liquids have advantages of degradation of skin irritation due to decomposition of stimulating substances by microorganisms or conversion into other substances, and fermentation products by microorganisms are also widely used because they have a good effect on skin.

For reference, a number of patent documents and articles are referenced and citations are indicated throughout this specification. The disclosures of the cited patent documents and papers are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

Japanese Patent Application Laid-Open No. 2002-187247 discloses an external preparation for skin comprising tilandia plant extract.

The present inventors also developed a product using various natural products for this purpose, and have been making efforts to improve skin whitening by using the extract of Beetle tilandia as a representative. Based on these existing data, the inventors of the present invention have confirmed that fermentation of barley tillandiae produces a lot of effective ingredients and that skin whitening effect is far superior to that of existing barley tillandiaea extracts, thus completing the present invention.

The above object is achieved by a cosmetic composition for skin whitening comprising a fermentation broth of Tillandia sylvia obtained by culturing Tillandia hyacinthus with yeast or lactic acid bacteria.

Preferably, the fermentation broth of the present invention is a fermentation broth obtained by cultivating tillandia sylvia with Saccharomyces cerevisiae as a yeast or Bifidobacterium as a lactic acid bacterium. .

Preferably, the fermented milk tillering fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the fermented milk tilland fermentation broth is characterized by inhibiting melanin production, inhibiting tyrosinase activity, and having a whitening effect.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >

The object of the present invention is also achieved by a cosmetic method characterized in that the cosmetic composition is applied to human skin to inhibit melanin production, inhibit tyrosinase activity, and exhibit a whitening effect.

The present invention relates to a cosmetic composition for skin whitening comprising a fermented bean tallow extract. The fermentation broth of the tiller tillian fermentation broth, which is an effective component contained in the cosmetic composition of the present invention, is a fermentation broth obtained by cultivating Tillandia tilla as a yeast or lactic acid bacterium, and has an excellent whitening effect excellent in melanin production inhibitory effect and tyrosinase activity inhibiting effect It is an ingredient having.

In addition, the cosmetic composition containing the fermented milk tilland ingredient of the present invention has a better whitening effect than the conventional cosmetic composition containing the tillyalia extract.

To achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising a fermented bean curd tile extract.

Tillandsia usneoides used as an active ingredient in the composition of the present invention is a plant of pineapple, and is known to originate in South America such as southeastern United States, Argentina, and Chile. It grows by climbing dead tree trunks, and has excellent ability to retain moisture and nutrients in leaves. At the same time, it is attracting attention as a plant that absorbs fine dust and heavy metals in the air and purifies the air. The small scale structure of leaves effectively absorbs heavy metals such as fine dust, lead, copper and zinc in the air, and is effective for air purification. The stem is dense with thin and long gray leaves 2-6cm long, and leaves are covered with small scales. Water is trapped between these scales, which absorb water, its nutrients, and heavy metals in the air. Absorbed water and nutrients are transported through the water tubes and gutters in the scales to various parts of the plant. Because of these characteristics, roots do not function and can survive without soil.

However, the researches on the role of air purification are currently being conducted, and the research as a whitening cosmetic composition is limited.

 The fermented milk tillering fermentation broth used in the present invention is a fermented fermented milk tilland obtained by culturing barley tillandiae using yeast or lactic acid bacteria.

Yeast cells used in the fermentation of barbitum tilandia according to the present invention belong to eukaryotic microorganisms (nuclear and cytoplasmic organisms separated by nuclear membranes) and have cell organelles such as cell walls, cell membranes, nuclei, mitochondria, and granules. Those with no distinction of sex mainly breed by budding, and among the many isolated yeasts, there is no efficacy, proliferation by fission or bud-fission instead of the method of prey, ascospore ), Or that no spore formation is observed, as well as a wide variety of yeast species. The size is usually 5-10 × 5-12 μm, 5-10 times the size of bacteria, the optimum pH is 4.5-5.5, and even survives in 1-10. Yeast surviving at pH 1 for 12 hours is stronger on acid than bacteria, so adjusting the pH of the medium to about 3.5 ~ 3.8 can inhibit bacterial contamination to some extent.

Yeast is slightly different depending on the species, but most of them grow well at 20 ~ 25 ℃. Usually, it is said that it is killed at above 50 ° C. However, it can be overcome to some extent by directly adding a growth factor which can not be synthesized above the temperature to the medium. Saccharomyces < RTI ID = 0.0 > In the case of S. cerevisiae , it is killed at above 40 ° C, but it can be prevented by adding oleic acid and ergosterol to the medium. Yeast cells themselves are unicellular plants and contain large amounts of proteins and trace minerals. A preferred yeast for use in fermenting the beard tilandia of the present invention is Saccharomyces cerevisiae.

The lactic acid bacterium used for fermenting barley tillansia of the present invention is a kind of microorganism useful for the human body and decomposes carbohydrate such as glucose or lactose to produce an organic acid such as lactic acid or acetic acid. Lactic acid bacteria make lactic acid from sugar is called fermentation, and through this fermentation process, fermented food is produced. In the present invention, it was used as a representative lactic acid bacteria using bifidobacteria tumefaciens from (Lactobacillus, Streptococcus, bifidobacteria tumefaciens, Pocono stock flow, Phedi O Lactococcus, Lactococcus) (Bifidobacterium).

In the present invention, a fermented bean tilandial fermentation broth is prepared as follows:

a) finely grinding the strontium titanate powder using 100 to 500 mesh;

b) adding 1 to 50 g / L of the tiller tillia powder prepared in step a) to a conventional microorganism culture solution, adding yeast strain or lactic acid bacteria in an amount of 10,000 to 100,000 cfu / L;

c) culturing the culture of step b) at 30-37 ° C in aerobic or anaerobic conditions at pH 5-7 for about 5-10 days; And

d) filtering the culture of step c) and aging at low temperature for 5 to 10 days.

In accordance with a preferred embodiment of the present invention, the fermented bean tilyland fermentation broth used in the present invention was prepared as follows:

Lactic acid bacteria or lactic acid bacteria are added at 50,000 cfu / L, and the mixture is aerated or mixed at 30 to 37 ° C at a pH of 5-7. It is cultured in a fermenter for about 7 days under anaerobic conditions. After culturing, a culture solution is obtained, and then the culture solution is filtered using 0.25 μM filter paper. The filtrate is further aged at low temperature for 10 days, filtered and concentrated to prepare the fermented bean tilyland fermentation broth of the present invention.

In the present invention, the fermented bean tilandosia fermentation broth is characterized by inhibiting tyrosinase activity and improving skin whitening inhibiting melanin production.

In the present invention, the content of the fermented milk tilland juice is 0.0001 - 30.0% (w / w), more preferably 0.001 - 20% (w / w) with respect to the total cosmetic composition. When the content of the fermented broth of tillandiae is less than 0.0001% (w / w), the skin whitening effect can not be expected to be remarkably improved. When the content of fermented broth tillandiae exceeds 30.0% There is no increase, and skin irritation is caused or there is a problem in the stability of the formulation.

The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the fermentation broth of tiliandthia as an active ingredient and can be prepared by conventional methods such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention also provides a makeup method characterized by applying a cosmetic composition for skin whitening comprising a fermented bean tallow extract to human skin to suppress tyrosinase activity and inhibit melanin formation.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. The cosmetic composition having an excellent skin whitening effect according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

When the cosmetic composition comprising the ferulic acid fermentation broth of the present invention is applied to human skin, tyrosinase activity and melanin production are inhibited, skin pigmentation is improved, and skin whitening effect can be obtained .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1. Using yeast Beard Tilandia  Yeast fermentation broth manufacturing

Washed with purified water and dried, and powdered tilandosia was finely ground using 300 mesh. This was added to a yeast culture solution (Potato Dextrose Broth, Akyumedia, USA) at 10 g / L. The yeast strain used for fermentation was Saccharomyces cerevisiae , which was added at 50,000 cfu / L per culture. The cultures were maintained at 30 ° C and pH 5-7 for 7 days using a 5L fermenter. After culturing, the culture broth was centrifuged to remove the cultures first, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged at a low temperature warehouse at 4 ° C for about 10 days. The filtrate was filtered again using a 0.25 μM filter and used in the present invention. The thus prepared fermentation broth is hereinafter referred to as " beef tilandia yeast fermentation broth. &Quot; The fermentation broth of tillerisha yeast used in Experimental Example was kept at a final concentration of 1 g / 100 ml.

Production Example 2. Production of fermentation broth of tilandia lactic acid bacterium using Bifidobacterium sp. Strain

After washing with purified water and drying, the dried tillandiae was pulverized and made fine using 300 mesh. This was added to the culture solution of lactic acid bacteria to be 10 g / L. Glucose was added as a carbon source, and Dipotassium phosphate was added as a pH regulator. Bifidobacterium was used as a lactic acid bacterium and added at 50,000 cfu / L per culture. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 5-7. After culturing, the culture was centrifuged, and the culture was firstly removed and finally filtered using 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days at a low temperature warehouse at 4 DEG C, and the filtrate was filtered again using a 0.25 mu M filter and used in the present invention. The thus-prepared fermentation broth is hereinafter referred to as " fermentation broth of fermented beans tilandia lactic acid bacteria ". The fermentation broth of tungstillia lactic acid bacteria used in the experiments was kept at a final concentration of 1 g / 100 ml.

Comparative Example  One. Beard Tilandia  Extract preparation

The beard tilandia was washed with purified water, dried and powdered. Then, 100 g was added to 0.6 L of 70% ethanol solution, and the mixture was heated and extracted with a reflux condenser equipped with a condenser for 4 hours. Then, the obtained extract was filtered with 300 mesh filter cloth, filtered through filter paper No. 5, and then the solvent was removed using a vacuum concentrator and lyophilized to obtain 5.5 g of dry weight. The extract thus obtained is hereinafter referred to as 'Tilandia tilia extract' and the tiland extract of beard tilandia is used at a final concentration of 1 g / 100 mL.

Experimental Example 1: Inhibition of tyrosinase activity of fermented broth

As a substrate, 40 μl of 1.5 mmol / l tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used. The fermentation broth of tiller tillia yeast prepared in Preparation Example 1 and the fermentation broth of tillish tiller fermentation broth prepared in Preparation Example 2 were each diluted with a 0.05 M sodium phosphate buffer solution (pH 6.8), and the samples (0.5% and 1.0% Lt; / RTI > To the solution was added 20 μl of tyrosinase (Sigma, 1500 U / ml), and the mixture was reacted at 37 ° C. for 15 minutes. Then, the mixture was reacted at 490 nm Absorbance was measured. As a comparative group, the barley tiland extract of Comparative Example 1 was used in place of the barley tilandia fermentation broth. Activity inhibition of tyrosinase was calculated by the following formula (1). The experimental results are shown in Table 1 below.

Inhibitory effect of tyrosinase activity of fermented bean curd Test substance concentration (%) Inhibition rate of tyrosinase activity (%) Beard tilandia yeast fermentation broth 0.5% 44.6 Beetle tilandia yeast fermentation broth 1.0% 61.6 Beef tilandia lactic acid fermentation broth 0.5% 52.9 Beef tilandia lactic acid fermentation broth 1.0% 75.8 Beard tiland extract 0.5% 30.6 Beard tillandiaea extract 1.0% 46.8

From the above results, it was found that the treatment with the fermentation broth of T. rubrum yeast and the fermentation broth of T. rubrum showed excellent inhibitory effect on tyrosinase activity compared with that of the T. rubrum extract (comparative group) It was found that the fermentation broth inhibited melanin production and could solve the problem of skin pigmentation.

Experimental Example 2: Melanin biosynthesis inhibition experiment of a fermented broth

B-16 cells (mouse melanoma cells, Korean cell line bank) were inoculated on a 6-well plate at 1 × 10 ⁵ cells / well and then cultured for 24 hours. Each of the wells was treated with 1% and 2% each of the fermentation broth of Tailrandia cyanobacterium and cultured for 3-4 days, followed by centrifugation of the culture solution of each well. The separated cells were dissolved in 500 μl of 1N NaOH solution in dimethylsulfoxide (DMSO) and heated in a 90 ° C water bath for 10 minutes. The absorbance of this supernatant was measured at 490 nm. As a control group, a fermented bean curd tilland fermentation broth was used. The inhibition of melanin formation was calculated according to the following equation (2), and the results are shown in Table 2.

Inhibitory Effect on Melanin Biosynthesis of Fermented Milk Thistle Test substance concentration (%) Melanin production inhibition rate (%) Beard tilandia yeast fermentation broth 0.5% 34.5 Beetle tilandia yeast fermentation broth 1.0% 78.1 Beef tilandia lactic acid fermentation broth 0.5% 44.5 Beef tilandia lactic acid fermentation broth 1.0% 82.9 Beard tiland extract 0.5% 33.6 Beard tillandiaea extract 1.0% 57.7

As a result, the inhibitory effect on the tyrosinase activity and the inhibitory effect on the melanin biosynthesis were superior to those of the control tilleria chrysanthemum extract (control group) when the fermentation broth of T. rubrum yeast and the fermentation broth of T. rubi From these results, it was found that the fermentation medium of barley tillandosia inhibits the melanin production, thereby solving the skin pigment problem. Based on the results of the above-described experimental examples, a cosmetic comprising the fermentation broth of the present invention is prepared and presented. However, the composition of the present invention is not intended to be limited to the following formulations. Any of the fermented bean curd tylanshi yeast fermentation broth or fermented bean curd tylanshia fermentation broth may be used as the fermentation broth of tiller tai cha used in the following prescription examples.

Prescription Example 1: Softening Longevity

Examples of the formulation of softening water in cosmetics containing a fermented bean curd tylanshia solution are as follows.

ingredient content(%) Beetle tilandiae fermentation broth 0.5 glycerin 5.0 1,3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense, 0.01 Distilled water Balance Sum 100

Prescription Example 2: Nourishing Lotion

Examples of prescription of the nutritional lotion among the cosmetics containing the fermented bean curd tylanshia are as follows.

ingredient Content (unit:% by weight) Beetle tilandiae fermentation broth 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Methyl paraben 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription Example 3: Nourishing cream

Examples of the formulas of the nutritional cream among the cosmetics containing the fermented bean curd tylanshia are as follows.

ingredient Content (unit:% by weight) Beetle tilandiae fermentation broth 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example  3: Cosmetics  Experiment of whitening effect of composition

The whitening effect test of the cosmetic composition of the present invention was conducted on 40 black Korean women of 20 to 40 years old. The test product was prepared by first preparing a product containing 3% by weight of fermented yeast fermented yeast and 3% by weight of a fermented fermented product of fermented beans tilandosia lactic acid, according to the prescription of Prescription Example 3, and containing the fermented bean tilandia fermented product , And the product containing 3 wt% of tiland extract was used as a comparative product. In the experiment, firstly, the control product and the product containing the fermented yeast fermented yeast were used for 20 subjects on each side of the face. Twenty subjects were compared with the products containing the fermented liquid of the fermented bean tilandia lactic acid bacteria Each side of the face was used for each subject. Minolta CR 300 was used for the measurement and it was measured for 8 weeks. The experimental results were compared with the L value taken at 8 weeks and the L value taken at 0 week. The results are shown in Table 6 below.

division Contrast product Compare Products Beetle tilandia yeast fermentation product Products of fermented bean curd tylandsia lactic acid bacteria DELTA L value 0.125 0.235 0.453 0.468

As shown in Table 6 above, the whitening effect of the product containing the fermented bean curd tile fermented product was higher than that of the control product. In addition, the whitening effect was much higher than that of the comparative product containing the barley tiland extract. In other words, it can be seen that the whitening effect is increased by fermenting the extract of Tyrandia cunea with whitening activity. Therefore, it can be seen that the cosmetic composition of the present invention has an excellent whitening effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

A cosmetic composition for skin whitening comprising a fermented bean tallow extract obtained by culturing beard tillandiae with yeast or lactic acid bacteria. The fermentation broth according to claim 1, wherein the fermentation broth of Tritanil hydrolyzate is obtained by cultivating Tritanthya hybrids with Saccharomyces cerevisiae as a yeast or Bifidobacterium as a lactic acid bacterium, Wherein the skin-whitening cosmetic composition is a cosmetic composition for skin whitening. The cosmetic composition for skin whitening according to claim 1, wherein the fermented milk tillandia fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Lt; RTI ID = 0.0 > cosmetic < / RTI > composition for skin whitening.