WO2019124862A1 - Skin wrinkle prevention and improvement composition containing schisandra chinensis extract as active ingredient - Google Patents

Skin wrinkle prevention and improvement composition containing schisandra chinensis extract as active ingredient Download PDF

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Publication number
WO2019124862A1
WO2019124862A1 PCT/KR2018/015746 KR2018015746W WO2019124862A1 WO 2019124862 A1 WO2019124862 A1 WO 2019124862A1 KR 2018015746 W KR2018015746 W KR 2018015746W WO 2019124862 A1 WO2019124862 A1 WO 2019124862A1
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extract
lotion
skin wrinkles
cosmetic composition
composition
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PCT/KR2018/015746
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French (fr)
Korean (ko)
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김동선
이영실
김지연
손은정
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한국 한의학 연구원
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Definitions

  • Schisandra (Schisandra chinensis ) extract as an active ingredient.
  • the skin becomes thinner (in the case of endogenous aging) due to intrinsic aging and photoaging due to various contaminants and ultraviolet light externally, and the elasticity is reduced. As the skin is exposed to ultraviolet rays, The keratinocytes and melanocytes are damaged by UVB.
  • Skin aging is divided into chronologic aging, which occurs in areas that are not exposed to sunlight, and actinic aging, which is a combination of chronologic aging and degenerative changes in the exposed parts of sunlight.
  • chronologic aging occurs in areas that are not exposed to sunlight
  • actinic aging which is a combination of chronologic aging and degenerative changes in the exposed parts of sunlight.
  • characteristic clinical signs of skin are fine wrinkles, atrophy of dermis, and decrease of subcutaneous fat layer.
  • coatse wrinkling and furrowing appear and abnormal elasticity material accumulates and the skin thickens and loosens like leather.
  • the phenomenon seen in chronic sunlight-damaged skin is an abnormal increase in the elastic elastosis and proteoglycan of the upper collagen of the dermis and a significant decrease in collagen, the main protein of the dermis.
  • the dermis consists of the majority of type I collagen, some type II collagen, elastin, proteoglycan, and fibronectin.
  • Collagen imparts strength and tension to the skin, which protects the skin from external stimuli or forces, and it accounts for 90% of the dermis.
  • Collagen reduction is closely related to skin aging.
  • Cells that constitute the skin have decreased cell activity due to endogenous aging, photoaging, and imperfect signal transduction, resulting in increased biosynthesis of matrix metalloproteinases (MMPs), enzymes that degrade skin tissues, decreased collagen biosynthesis, And melanin production, which causes skin blackening, is known to increase.
  • MMPs matrix metalloproteinases
  • MMPs that decompose collagen which is a substrate substance constituting the skin
  • substances that can thicken the skin by proliferating skin cells or increasing the matrix substances constituting the skin, inhibiting melanin biosynthesis And the like are found to alleviate skin symptoms such as wrinkles, loss of elasticity, and skin blackening.
  • Schizandra chinensis is a dicotyledonous plant that is a magnolia plant of the Magnoliaceae. It grows mainly in valleys, the stem is brown and has the property of climbing trees. Leaves are alternate phyllotaxis, wide oval, long oval or ovate, with hairs on the back vein and tooth-like sawtooth on the edge. The flower is bloomed in June to July, is unisolated, and has a slightly reddish yellowish white color. After the flowering, the flower bud of the female flower grows to 3 ⁇ 5cm in length and the fruit runs on the water. Fruits are berries and they are almost round and have several spines.
  • Korean Patent Laid-Open Publication No. 2013-0087938 discloses a cosmetic composition for improving wrinkles containing an extract of Omija
  • Korean Patent Laid-Open Publication No. 2017-0076134 discloses a cosmetic composition containing gum arabicum extract as an active ingredient
  • a wrinkle preventing and anti-inflammatory composition has been disclosed, but a composition for preventing or improving wrinkles of skin containing the extract of the present invention as an active ingredient has not been disclosed.
  • the present invention provides a composition for preventing or ameliorating skin wrinkles comprising an extract of Omija as an active ingredient, and a composition for preventing or treating wrinkles of the omija according to the present invention, Analysis of the collagenase inhibitory activity of the extract showed that the collagenase inhibitory activity of the Omija leaf or stem extract was superior to that of the Omiza fruit extract and that the Omiza leaf extract had a higher expression level of Pro-MMP-1 than the Omiza fruit extract The present invention has been completed.
  • the present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
  • the present invention also provides a health functional food composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
  • the present invention also provides a quasi-drug composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
  • the present invention relates to a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
  • the extract of Omija of the present invention is excellent in collagenase inhibitory activity, and in particular, it is more effective than the extract of Omija extract or collagenase Inhibitory activity, and the effect of Omiza leaf extract on pro-MMP-1 expression is further reduced than that of Omiza fruit extract. Therefore, the extract of Omima leaf or stem of the present invention can be applied to a cosmetic for improving skin wrinkles or a health functional food.
  • FIG. 1 shows the results of cytotoxicity of extracts (fruit, stem and leaf) extracts according to the present invention.
  • FIG. 2 shows the results of confirming the collagenase inhibitory activity (%) of the extracts of Omija (fruit, stem and leaf) according to the present invention.
  • EGCG epigallocatechin gallate
  • FIG. 3 shows the effect of reducing the expression level of Pro-MMP-1 according to the treatment of Omiza and Omiza leaf extracts.
  • P ⁇ 0.01, **, **, *** indicate that the expression level of Pro-MMP-1 in the TNF- ⁇ -treated group was statistically significantly increased compared with the normal group
  • the expression level of Pro-MMP-1 in the experimental group treated with Schizandra chinensis L. or Leaf extract compared to the group treated with TNF- ⁇ was statistically significantly decreased, p ⁇ 0.05, ** p ⁇ 0.01 , *** is p ⁇ 0.001.
  • the present invention relates to a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
  • the above-mentioned Omiza extract is preferably a leaf, a stem or a mixed extract thereof of Omija, but is not limited thereto.
  • wrinkle formation is caused by reduction of collagen, and reduction of the collagen may be caused by excessive oxidative stress or ultraviolet irradiation.
  • the above-mentioned Omiza extract has a collagenase inhibitory activity.
  • the Omiza extract may be prepared by a method including, but not limited to, the following steps:
  • step (3) The step of extracting the filtered Schizandra chinensis from step (2) is concentrated under reduced pressure and dried to produce an extract of Schizandra chinensis.
  • the extraction solvent is preferably selected from water, a C 1 -C 4 lower alcohol or a mixture thereof, more preferably ethanol, even more preferably 70% (v / v) ethanol But is not limited thereto.
  • any conventional method known in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction may be used.
  • the extraction solvent is preferably added by 1 to 20 times the weight of dried omiza, more preferably 5 to 15 times.
  • the extraction temperature is preferably 4 to 50 ° C, but is not limited thereto.
  • the extraction time is preferably 0.5 to 10 hours, more preferably 0.5 to 5 hours, most preferably 1 hour, but not always limited thereto.
  • the drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.
  • the cosmetic composition according to one embodiment of the present invention may be used as an external ointment for skin, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and Body And a cleanser.
  • the cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.
  • the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.
  • lactose When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propellants such as hydrocarbons, propane-butanes or dimethyl ethers.
  • a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.
  • the formulation of the cosmetic composition of the present invention is a suspension
  • a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
  • the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.
  • the cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .
  • an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .
  • the cosmetic composition of the present invention can be directly applied to skin using biodegradable micro needles or patches.
  • the present invention also relates to a health functional food composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
  • the health functional food composition of the present invention When used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method.
  • the amount of the active ingredient to be mixed can be suitably used depending on the purpose of use (prevention or improvement).
  • the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced.
  • the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount of more than the above range.
  • the kind of the health functional food There is no particular limitation on the kind of the health functional food.
  • foods to which the health functional food composition can be added include meat products, sausages, breads, chocolate, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, dairy products including ice cream, various soups, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.
  • the health functional food composition of the present invention can be produced as a food, particularly a functional food.
  • the functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings.
  • the active ingredient when it is made of a drink, it may contain, in addition to the active ingredient, a natural carbohydrate or a flavoring agent as an additional ingredient.
  • the natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, For example, xylitol, sorbitol, erythritol, etc.).
  • the flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).
  • the above health functional food composition it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like.
  • the ratio of the above-mentioned ingredients is not critical, it is generally selected in the range of 0.01 to 0.1 part by weight based on 100 parts by weight of the health functional food composition of the present invention.
  • the present invention also relates to a quasi-drug composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
  • the "quasi-drug composition” is a fiber, a rubber product or the like used for treating, alleviating, treating or preventing a disease of a human or an animal, Or products similar to those which are not machinery, preparations used for sterilization, insecticides and similar uses for the prevention of infections, for the purpose of diagnosis, treatment, alleviation, treatment or prevention of diseases of human beings or animals Means an article other than an apparatus, a machine or an apparatus, and an article other than an apparatus, a machine or an apparatus used for the purpose of giving a pharmacological effect to the structure and function of a person or an animal, But not limited to, personal hygiene products.
  • the quasi-drug composition of the present invention may be contained at various weight%, preferably 0.00001 to 10% by weight, as long as it can exhibit a skin wrinkle-reducing effect.
  • MC / 9 cells were diluted for each concentration for 24 hours, and cell viability was measured at 550 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA) using the MTT assay.
  • the collagenase inhibitory activity was determined by adding 4 mM CaCl 2 to 0.1M tris-HCl buffer (pH 7.5) in a reaction vessel and adding 4-phenylaz-o-benzyloxycarbonyl-Pro-Leu-Gly-Pro- ), 0.15 ml of collagenase (0.2 mg / ml) was added to a mixture of 0.25 ml of the substrate solution and 0.1 ml of the solution of each concentration (100, 200, 400, 800 and 1200 / / ml) After standing, 0.5 ml of 6% citric acid was added to quench the reaction. 1.5 ml of ethyl acetate was added and the absorbance at 320 nm was measured. The collagenase inhibitory activity was calculated as the absorbance reduction rate of the sample solution added and the non-added sample.
  • the extract of the present invention inhibited collagenase activity.
  • the extract of the omija leaf or stem showed statistically significant collagenase activity Respectively.
  • both the Omiza fruit and leaf extract had an effect of reducing the expression amount of Pro-MMP-1 increased by TNF- ⁇ , and in particular, the Omiza leaf extract was lower MMP-1 < SEP >

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Abstract

The present invention relates to a composition which contains Schisandra chinensis as a main ingredient and is for preventing and improving skin wrinkles. The Schisandra chinensis extract of the present invention has excellent collagenase inhibition activity. In particular, Schisandra chinensis leaf and stem extracts have superior collagenase inhibition activity to Schisandra chinensis fruit extract and Schisandra chinensis leaf extract has a greater effect in reducing the expression of Pro-MMP-1 than Schisandra chinensis fruit extract. Consequently, the composition of the present invention can be utilized to good effect in a cosmetic composition for improving skin wrinkles and can also be utilized as a functional health food for improving skin wrinkles as a beauty food.

Description

오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 조성물Composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient
본 발명은 오미자(Schisandra chinensis) 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 조성물에 관한 것이다.The invention Schisandra (Schisandra chinensis ) extract as an active ingredient.
인간의 피부는 시간이 지남에 따라 내적으로는 신진대사를 조절하는 각종 호르몬의 분비가 감소하고, 면역 세포의 기능과 세포들의 활성이 저하되어 생체에 필요한 면역 단백질 및 생체 구성 단백질들의 생합성이 줄어들게 되어 생기는 내인성 노화 (intrinsic aging)와, 외적으로는 각종 오염물질과 자외선에 의한 광노화(photoaging)에 의해, 피부가 얇아지며(내인성 노화의 경우), 탄력이 감소하게 되며, 자외선 조사에 노출됨에 따라 표피 내 각질형성세포와 멜라닌 세포가 UVB에 의해 세포손상을 받게 된다. Over time, the secretion of various hormones that regulate metabolism is reduced, and the function of immune cells and the activity of cells are lowered, so that the biosynthesis of immune proteins and biologic proteins necessary for the living body is reduced The skin becomes thinner (in the case of endogenous aging) due to intrinsic aging and photoaging due to various contaminants and ultraviolet light externally, and the elasticity is reduced. As the skin is exposed to ultraviolet rays, The keratinocytes and melanocytes are damaged by UVB.
피부 노화는 태양광선에 노출되지 않는 부위에서 나타나는 연대학적 노화 (chronologic aging)와 태양광선의 노출부에서 일어나는 퇴행성 변화가 연대학적 노화와 복합되어 일어나는 광인성 노화(actinic ageing)로 구분된다. 연대학적 노화에서는 특징적인 임상 피부소견으로 미세한 주름, 진피의 위축, 피하지방층의 감소 등이 관찰된다. 광노화 과정에서는 거칠고 깊은 주름(coatse wrinkling and furrowing)이 나타나고 비정상적인 탄력질양 물질(elastotic material)이 축적되어 피부가 가죽같이 두터워지며 느슨해진다. 만성적 일광손상을 입은 피부에서 볼 수 있는 현상은 진피의 상부 쪽 교원질의 비정상적인 탄력질양 물질의 침착(solar elastosis)과 프로테오글리칸(proteoglycan)이 증가되고 진피의 주단백질인 콜라겐이 현저히 감소되는 것이다. 일반적으로 진피층은 대다수의 제1형 콜라겐(type I collagen)과 약간의 제2형 콜라겐(type II collagen), 엘라스틴(elastin), 프로테오글리칸(proteoglycan), 피브로넥틴(fibronectin) 등으로 구성되어 있다. 콜라겐은 피부에 강도와 장력을 부여하며 이로 인해 외부의 자극이나 힘으로부터 피부를 보호하는 역할을 하며 진피층의 90%를 차지하고 있어 콜라겐의 감소는 피부의 노화와 밀접한 관계가 있다. 피부를 구성하는 세포에서는 내인성 노화나, 광노화에 의해 세포활성이 떨어지고, 신호전달 체계가 불완전해지면서 피부조직을 분해하는 효소인 MMPs(matrix metalloproteinases)의 생합성이 증가하고, 콜라겐의 생합성이 감소하여 주름살이 생기고 탄력이 감소하고, 피부 흑화를 초래하는 멜라닌 생성이 증가하는 것으로 알려져 있다. 따라서 피부 세포를 증식시키거나, 피부를 구성하는 기질물질을 증가시킴으로써 피부를 두껍게 할 수 있는 물질, 피부를 구성하는 기질 물질인 콜라겐을 분해하는 MMPs의 생합성을 억제할 수 있는 물질, 멜라닌 생합성을 억제할 수 있는 물질 등이 주름, 탄력 상실, 피부흑화 등의 피부 증상을 완화시키는 것으로 밝혀져 있다.Skin aging is divided into chronologic aging, which occurs in areas that are not exposed to sunlight, and actinic aging, which is a combination of chronologic aging and degenerative changes in the exposed parts of sunlight. In chronological aging, characteristic clinical signs of skin are fine wrinkles, atrophy of dermis, and decrease of subcutaneous fat layer. In the photoaging process, coatse wrinkling and furrowing appear and abnormal elasticity material accumulates and the skin thickens and loosens like leather. The phenomenon seen in chronic sunlight-damaged skin is an abnormal increase in the elastic elastosis and proteoglycan of the upper collagen of the dermis and a significant decrease in collagen, the main protein of the dermis. In general, the dermis consists of the majority of type I collagen, some type II collagen, elastin, proteoglycan, and fibronectin. Collagen imparts strength and tension to the skin, which protects the skin from external stimuli or forces, and it accounts for 90% of the dermis. Collagen reduction is closely related to skin aging. Cells that constitute the skin have decreased cell activity due to endogenous aging, photoaging, and imperfect signal transduction, resulting in increased biosynthesis of matrix metalloproteinases (MMPs), enzymes that degrade skin tissues, decreased collagen biosynthesis, And melanin production, which causes skin blackening, is known to increase. Therefore, it can inhibit the biosynthesis of MMPs that decompose collagen, which is a substrate substance constituting the skin, substances that can thicken the skin by proliferating skin cells or increasing the matrix substances constituting the skin, inhibiting melanin biosynthesis And the like are found to alleviate skin symptoms such as wrinkles, loss of elasticity, and skin blackening.
한편, 오미자나무(Schizandra chinensis )는 쌍떡잎 식물로 목련목 오미자과의 낙엽 덩굴식물이다. 주로 산골짜기에서 자라며, 줄기는 갈색이고 나무를 기어 오르는 성질이 있다. 잎은 어긋나고 넓은 타원형, 긴 타원형 또는 달걀 모양이며 뒷면 잎맥 위에는 털이 있고 가장자리에 치아 모양의 톱니가 있다. 꽃은 6∼7월에 피고 단성화이며 약간 붉은빛이 도는 황백색이다. 꽃이 핀 다음 암꽃의 꽃턱은 길이 3∼5cm로 자라서 열매가 수상으로 달린다. 열매는 장과(漿果)로 거의 둥글고 이삭 모양으로 여러 개가 달린다. 8∼9월에 홍색으로 익으며 1∼2개의 홍갈색 종자가 들어 있다. 어린 순은 나물로 먹는다. 열매에 신맛, 단맛, 쓴맛, 짠맛, 매운맛의 다섯 가지 맛이 섞여 있어 오미자라 하며 약용한다. 한방에서 자양(滋養), 강장, 진해(鎭咳), 거담(祛痰), 지한(止汗) 등의 효력이 있어 해수·유정(遺精), 구갈, 도한(盜汗), 급성간염 등에 처방한다. 민간에서는 오미자차를 만들어 마시며 술도 담근다. On the other hand, Schizandra chinensis ) is a dicotyledonous plant that is a magnolia plant of the Magnoliaceae. It grows mainly in valleys, the stem is brown and has the property of climbing trees. Leaves are alternate phyllotaxis, wide oval, long oval or ovate, with hairs on the back vein and tooth-like sawtooth on the edge. The flower is bloomed in June to July, is unisolated, and has a slightly reddish yellowish white color. After the flowering, the flower bud of the female flower grows to 3 ~ 5cm in length and the fruit runs on the water. Fruits are berries and they are almost round and have several spines. It grows in red color from August to September and contains 1 to 2 reddish brown seeds. Younger children eat them as herbs. The fruit is mixed with the five flavors of sour, sweet, bitter, salty, and spicy. It has the effect of nourishing, gangjang, Jinhae, gadam, and perspiration in one room and it is prescribed in seawater, oilseed, gogal, dohan (sweat), acute hepatitis etc. . In the private sector, they make and drink omija tea and drink.
오미자 관련 선행기술로는 한국공개특허 제2013-0087938호에 오미자 추출물을 포함하는 주름개선용 화장료 조성물이 공개되어 있고, 한국공개특허 제2017-0076134호에 발아 오미자씨 추출물을 유효성분으로 함유하는 피부 주름 방지 및 항염 조성물이 개시되어 있으나, 본 발명의 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 조성물은 개시된 바 없다. As prior art related to omija, Korean Patent Laid-Open Publication No. 2013-0087938 discloses a cosmetic composition for improving wrinkles containing an extract of Omija, Korean Patent Laid-Open Publication No. 2017-0076134 discloses a cosmetic composition containing gum arabicum extract as an active ingredient A wrinkle preventing and anti-inflammatory composition has been disclosed, but a composition for preventing or improving wrinkles of skin containing the extract of the present invention as an active ingredient has not been disclosed.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 조성물을 제공하고, 본 발명에 따른 오미자 부위 별(열매, 줄기, 잎) 추출물의 콜라게나아제 저해 활성을 분석한 결과, 오미자 열매 추출물보다 오미자 잎 또는 줄기 추출물의 콜라게나아제 저해 활성이 더 우수하고, 오미자 잎 추출물이 오미자 열매 추출물보다 Pro-MMP-1의 발현량을 더 감소시키는 효과가 있다는 것을 확인함으로써, 본 발명을 완성하였다.The present invention provides a composition for preventing or ameliorating skin wrinkles comprising an extract of Omija as an active ingredient, and a composition for preventing or treating wrinkles of the omija according to the present invention, Analysis of the collagenase inhibitory activity of the extract showed that the collagenase inhibitory activity of the Omija leaf or stem extract was superior to that of the Omiza fruit extract and that the Omiza leaf extract had a higher expression level of Pro-MMP-1 than the Omiza fruit extract The present invention has been completed.
상기 목적을 달성하기 위하여, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름 개선용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
또한, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 건강기능식품 조성물을 제공한다.The present invention also provides a health functional food composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
또한, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 의약외품 조성물을 제공한다.The present invention also provides a quasi-drug composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름 개선용 화장료 조성물에 관한 것으로, 본 발명의 오미자 추출물은 콜라게나아제 저해 활성이 우수하며, 특히 오미자 열매 추출물보다 오미자 잎 또는 줄기 추출물의 콜라게나아제 저해 활성이 더 우수하며, 오미자 잎 추출물이 오미자 열매 추출물보다 Pro-MMP-1의 발현량을 더 감소시키는 효과가 있다. 따라서 본 발명의 오미자 잎 또는 줄기 추출물은 피부 주름 개선용 화장품 또는 건강기능식품에 활용할 수 있다.The present invention relates to a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient. The extract of Omija of the present invention is excellent in collagenase inhibitory activity, and in particular, it is more effective than the extract of Omija extract or collagenase Inhibitory activity, and the effect of Omiza leaf extract on pro-MMP-1 expression is further reduced than that of Omiza fruit extract. Therefore, the extract of Omima leaf or stem of the present invention can be applied to a cosmetic for improving skin wrinkles or a health functional food.
도 1은 본 발명에 따른 오미자 부위별(열매, 줄기 및 잎) 추출물의 세포 독성을 확인한 결과이다.FIG. 1 shows the results of cytotoxicity of extracts (fruit, stem and leaf) extracts according to the present invention.
도 2는 본 발명에 따른 오미자 부위별(열매, 줄기 및 잎) 추출물의 콜라게나아제 저해 활성(%)을 확인한 결과이다. EGCG(epigallocatechin gallate)는 양성대조군이다.FIG. 2 shows the results of confirming the collagenase inhibitory activity (%) of the extracts of Omija (fruit, stem and leaf) according to the present invention. EGCG (epigallocatechin gallate) is a positive control.
도 3은 오미자 열매 및 오미자 잎 추출물의 처리에 따른 Pro-MMP-1의 발현량 감소 효과를 확인한 것이다. ##은 정상군(-)에 비해 TNF-α를 처리한 군에서의 Pro-MMP-1의 발현량이 통계적으로 유의미하게 증가하였다는 것으로, p<0.01이고, *, **, ***은 TNF-α를 처리한 군 대비 오미자 열매 또는 잎 추출물을 처리한 실험군에서의 Pro-MMP-1의 발현량이 통계적으로 유의미하게 감소하였다는 것으로, *은 p<0.05이고, **은 p<0.01이며, ***은 p<0.001이다. FIG. 3 shows the effect of reducing the expression level of Pro-MMP-1 according to the treatment of Omiza and Omiza leaf extracts. (P <0.01), **, **, *** indicate that the expression level of Pro-MMP-1 in the TNF-α-treated group was statistically significantly increased compared with the normal group The expression level of Pro-MMP-1 in the experimental group treated with Schizandra chinensis L. or Leaf extract compared to the group treated with TNF-α was statistically significantly decreased, p <0.05, ** p <0.01 , *** is p <0.001.
본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름 개선용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
상기 오미자 추출물은 오미자의 잎, 줄기 또는 이들의 혼합 추출물인 것이 바람직하지만 이에 한정하지 않는다. The above-mentioned Omiza extract is preferably a leaf, a stem or a mixed extract thereof of Omija, but is not limited thereto.
본 발명에서 주름 생성은 콜라겐의 감소로 인해 발생하는 것이며, 상기 콜라겐의 감소는 과도한 산화 스트레스나 자외선 조사가 원인일 수 있다. 상기 오미자 추출물은 콜라게나아제 저해 활성을 갖는 것을 특징이다.In the present invention, wrinkle formation is caused by reduction of collagen, and reduction of the collagen may be caused by excessive oxidative stress or ultraviolet irradiation. The above-mentioned Omiza extract has a collagenase inhibitory activity.
상기 오미자 추출물은 하기의 단계를 포함하는 방법에 의해 제조할 수 있으나, 이에 한정하지 않는다:The Omiza extract may be prepared by a method including, but not limited to, the following steps:
(1) 오미자 잎 또는 줄기의 건조분말에 추출용매를 가하여 추출하는 단계;(1) extracting a dried powder of Schizandra chinensis or stem by adding an extraction solvent;
(2) 단계 (1)의 오미자 추출물을 여과하는 단계; 및 (2) filtering out the Schizandra chinensis extract of step (1); And
(3) 단계 (2)의 여과한 오미자 추출물을 감압 농축하고 건조하여 오미자 추출물을 제조하는 단계. (3) The step of extracting the filtered Schizandra chinensis from step (2) is concentrated under reduced pressure and dried to produce an extract of Schizandra chinensis.
상기 단계 (1)에서 추출용매는 물, C1~C4의 저급 알코올 또는 이들의 혼합물 중에서 선택하는 것이 바람직하며, 더 바람직하게는 에탄올이고, 더욱더 바람직하게는 70%(v/v) 에탄올이지만 이에 한정하지 않는다.In step (1), the extraction solvent is preferably selected from water, a C 1 -C 4 lower alcohol or a mixture thereof, more preferably ethanol, even more preferably 70% (v / v) ethanol But is not limited thereto.
상기 제조방법에 있어서, 추출방법은 여과법, 열수 추출, 침지 추출, 환류 냉각 추출 및 초음파 추출 등의 당 업계에 공지된 모든 통상적인 방법을 이용할 수 있다. 상기 추출용매는 건조된 오미자 중량의 1~20배 첨가하여 추출하는 것이 바람직하며, 더 바람직하게는 5~15배 첨가하는 것이다. 추출온도는 4~50℃인 것이 바람직하나 이에 한정하지 않는다. 또한, 추출시간은 0.5~10시간인 것이 바람직하며, 0.5~5시간이 더욱 바람직하고, 1시간이 가장 바람직하나 이에 한정하지 않는다. 상기 방법에 있어서, 단계 (3)의 감압농축은 진공 감압 농축기 또는 진공회전증발기를 이용하는 것이 바람직하나 이에 한정하지 않는다. 또한, 건조는 감압건조, 진공건조, 비등건조, 분무 건조 또는 동결 건조하는 것이 바람직하나 이에 한정하지 않는다.In the above production method, any conventional method known in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction may be used. The extraction solvent is preferably added by 1 to 20 times the weight of dried omiza, more preferably 5 to 15 times. The extraction temperature is preferably 4 to 50 ° C, but is not limited thereto. The extraction time is preferably 0.5 to 10 hours, more preferably 0.5 to 5 hours, most preferably 1 hour, but not always limited thereto. In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in the step (3), but it is not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.
본 발명의 일 구현 예에 따른 화장료 조성물은 피부 외용 연고, 크림, 유연화장수, 영양화장수, 팩, 에센스, 헤어토닉, 샴푸, 린스, 헤어 컨디셔너, 헤어 트리트먼트, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처 로션, 영양로션, 마사지 크림, 영양크림, 모이스처 크림, 핸드 크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징 폼, 클렌징로션, 클렌징크림, 바디 로션 및 바디 클렌저로 이루어지는 군으로부터 선택된 어느 하나의 제형을 가질 수 있으나, 이에 제한되지 않는다. 이들 각 제형으로 이루어진 화장료 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.The cosmetic composition according to one embodiment of the present invention may be used as an external ointment for skin, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and Body And a cleanser. However, the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라가칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.
본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가로 클로로플루오로히드로카본, 프로판-부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propellants such as hydrocarbons, propane-butanes or dimethyl ethers.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.
본 발명의 화장료 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
본 발명의 화장료 조성물의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 아세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is an interfacial-active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.
본 발명의 화장료 조성물은 형광물질, 살진균제, 굴수성 유발물질, 보습제, 방향제, 방향제 담체, 단백질, 용해화제, 당 유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .
본 발명의 화장료 조성물은 생분해성 마이크로 니들 또는 패치 등을 이용하여 피부에 직접 적용할 수 있다.The cosmetic composition of the present invention can be directly applied to skin using biodegradable micro needles or patches.
또한, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 건강기능식품 조성물에 관한 것이다.The present invention also relates to a health functional food composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
본 발명의 건강기능식품 조성물을 식품첨가물로 사용하는 경우, 상기 건강기능식품 조성물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효 성분의 혼합양은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다. 일반적으로, 식품 또는 음료의 제조시 본 발명의 건강기능식품 조성물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나 건강을 목적으로 하는 장기간의 섭취의 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로 사용될 수 있다.When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably used depending on the purpose of use (prevention or improvement). Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in the case of long-term intake intended for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount of more than the above range.
상기 건강기능식품의 종류에 특별한 제한은 없다. 상기 건강기능식품 조성물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 수프, 음료수, 차 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the kind of the health functional food. Examples of foods to which the health functional food composition can be added include meat products, sausages, breads, chocolate, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, dairy products including ice cream, various soups, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.
또한, 본 발명의 건강기능식품 조성물은 식품, 특히 기능성 식품으로 제조될 수 있다. 본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함할 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등), 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다.In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when it is made of a drink, it may contain, in addition to the active ingredient, a natural carbohydrate or a flavoring agent as an additional ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, For example, xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).
상기 건강기능식품 조성물 이외에 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다. 이러한 상기 첨가되는 성분의 비율은 크게 중요하진 않지만 본 발명의 건강기능식품 조성물 100 중량부에 대하여, 0.01 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected in the range of 0.01 to 0.1 part by weight based on 100 parts by weight of the health functional food composition of the present invention.
또한, 본 발명은 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 의약외품 조성물에 관한 것이다.The present invention also relates to a quasi-drug composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
본 발명에서, "의약외품 조성물"은 사람이나 동물의 질병을 치료, 경감, 처치 또는 예방할 목적으로 사용되는 섬유, 고무제품 또는 이와 유사한 것, 인체에 대한 작용이 약하거나 인체에 직접 작용하지 않으며, 기구 또는 기계가 아닌 것과 이와 유사한 것, 감염 예방을 위하여 살균, 살충 및 이와 유사한 용도로 사용되는 제제 중 하나에 해당하는 물품으로서, 사람이나 동물의 질병을 진단, 치료, 경감, 처치 또는 예방할 목적으로 사용하는 물품 중 기구, 기계 또는 장치가 아닌 것 및 사람이나 동물의 구조와 기능에 약리학적 영향을 줄 목적으로 사용하는 물품 중 기구, 기계 또는 장치가 아닌 것을 제외한 물품을 의미하며, 구체적으로 피부 외용제 또는 개인위생용품일 수 있으나, 이에 제한되지 않는다. 본 발명의 의약외품 조성물은 피부 주름 개선 효과를 나타낼 수 있다면 다양한 중량%로 포함할 수 있으며, 바람직하게는 0.00001 내지 10 중량%로 포함할 수 있다.In the present invention, the "quasi-drug composition" is a fiber, a rubber product or the like used for treating, alleviating, treating or preventing a disease of a human or an animal, Or products similar to those which are not machinery, preparations used for sterilization, insecticides and similar uses for the prevention of infections, for the purpose of diagnosis, treatment, alleviation, treatment or prevention of diseases of human beings or animals Means an article other than an apparatus, a machine or an apparatus, and an article other than an apparatus, a machine or an apparatus used for the purpose of giving a pharmacological effect to the structure and function of a person or an animal, But not limited to, personal hygiene products. The quasi-drug composition of the present invention may be contained at various weight%, preferably 0.00001 to 10% by weight, as long as it can exhibit a skin wrinkle-reducing effect.
이하, 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다. Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.
실시예Example 1. 오미자 잎, 줄기 및 열매 추출물의 제조 1. Manufacture of Omija leaf, stem and fruit extract
본 발명의 1kg의 오미자 잎, 1kg의 오미자 줄기 또는 1kg의 오미자 열매 각각에 대하여, 15ℓ의 70%(v/v) 에탄올을 가하고, 85℃에서 3시간 동안 추출한 후, 여과한 액을 45℃에서 감압 농축, 건조하여 오미자 잎 추출물, 오미자 줄기 추출물 또는 오미자 열매 추출물을 수득하였다.15 liters of 70% (v / v) ethanol was added to 1 kg of the omza leaf of the present invention, 1 kg of Omija stem or 1 kg of Omiza fruit, extracted at 85 ° C for 3 hours, Concentrated under reduced pressure, and dried to obtain an Omija leaf extract, an Omija stem extract or an Omija fruit extract.
실시예Example 2. 세포  2. Cells 생존률Survival rate 분석 analysis
MC/9 세포에 24시간 동안 각각의 시료를 농도별로 희석하여 처리하고, MTT 어세이 기법으로 세포 생존율을 550nm에서 마이크로플레이트 리더(Molecular Devices, Sunnyvale, CA, USA)로 측정하였다. MC / 9 cells were diluted for each concentration for 24 hours, and cell viability was measured at 550 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA) using the MTT assay.
그 결과, 도 1에 개시한 바와 같이 오미자 열매, 잎 및 줄기 추출물 모두 세포독성이 거의 나타나지 않았다. As a result, as shown in Fig. 1, the extracts of Omiza fruit, leaf and stem showed almost no cytotoxicity.
실시예Example 3. 오미자 잎, 줄기 및 열매 추출물의  3. Obiza leaf, stem and fruit extract 콜라게나아제Collagenase 저해 활성 확인 Confirm inhibitory activity
상기 실시예 1에서 얻은 시료들의 피부주름 개선 효과를 확인하기 위하여, 콜라게나아제(Collageanse) 저해 활성시험을 실시하였다. 콜라게나아제 저해 활성은 반응구 0.1M tris-HCl buffer(pH 7.5)에 4mM CaCl2를 첨가하여, 4-phenylaz-o-benzyloxycarbonyl-Pro-Leu-Gly- Pro-D-Arg(0.3mg/㎖)를 녹인 기질액 0.25㎖ 및 시료 농도별(100, 200, 400, 800 및 1200㎍/㎖) 용액 0.1㎖의 혼합액에 콜라게나아제(0.2mg/㎖) 0.15㎖를 첨가하여 실온에서 20분 동안 정치한 후 6% 시트르산 0.5㎖을 넣어 반응을 정지시킨 후, 에틸아세테이트 1.5㎖을 첨가하여 320nm에서 흡광도를 측정하였다. 콜라게나아제 저해활성은 시료용액의 첨가구와 무첨가구의 흡광도 감소율로 계산되었다. In order to confirm the effect of improving the skin wrinkles of the samples obtained in Example 1, a collagenase inhibitory activity test was carried out. The collagenase inhibitory activity was determined by adding 4 mM CaCl 2 to 0.1M tris-HCl buffer (pH 7.5) in a reaction vessel and adding 4-phenylaz-o-benzyloxycarbonyl-Pro-Leu-Gly-Pro- ), 0.15 ml of collagenase (0.2 mg / ml) was added to a mixture of 0.25 ml of the substrate solution and 0.1 ml of the solution of each concentration (100, 200, 400, 800 and 1200 / / ml) After standing, 0.5 ml of 6% citric acid was added to quench the reaction. 1.5 ml of ethyl acetate was added and the absorbance at 320 nm was measured. The collagenase inhibitory activity was calculated as the absorbance reduction rate of the sample solution added and the non-added sample.
그 결과, 도 2에 개시한 바와 같이, 본 발명의 오미자 추출물이 콜라게나아제 활성을 저해하는 것을 확인하였으며, 특히, 오미자 잎 또는 줄기 추출물은 오미자 열매 추출물에 비해 통계적으로 유의미하게 콜라게나아제 활성을 저해하는 것을 확인하였다. As a result, as shown in Fig. 2, it was confirmed that the extract of the present invention inhibited collagenase activity. In particular, the extract of the omija leaf or stem showed statistically significant collagenase activity Respectively.
실시예Example 4. 오미자 잎 및 열매 추출물의 Pro- 4. Effect of Pro- MMPMMP (Matrix (Matrix metalloproteinasemetalloproteinase )-1 감소 효과 확인) -1 Decrease effect
48웰 플레이트에 세포를 3×104 세포가 되도록 접종하고 10% 혈청 DMEM 배지 및 37℃의 조건에서 24시간 동안 배양한 후, 배지를 제거하고 인산완충용액(PBS)으로 세척한 후 오미자 열매 및 잎 에탄올 추출물과 10ng/㎖의 TNF-α를 처리하여 16시간 동안 배양하였다. 16시간 배양 후 세포배양액을 확보하여 상업적으로 판매적하는 Pro-MMP-1(R&D)를 이용하여 Pro-MMP-1 농도를 측정하여 생성억제율(%)로 계산하였다. Cells were inoculated into 48-well plates at a density of 3 × 10 4 cells, cultured for 24 hours at a temperature of 37 ° C. in a 10% serum DMEM medium, and then the medium was removed and washed with phosphate buffered saline (PBS) Leaf ethanol extract and 10 ng / ml of TNF-? Were cultured for 16 hours. Pro-MMP-1 concentration was measured using commercially available Pro-MMP-1 (R & D) after cell culture for 16 hours.
그 결과, 도 3에 개시한 바와 같이 오미자 열매 및 잎 추출물 둘 다 TNF-α에 의해 증가된 Pro-MMP-1의 발현량을 감소시키는 효과가 있었으며, 특히 오미자 잎 추출물은 오미자 열매 추출물에 비해 낮은 농도에서도 통계적으로 유의미하게 Pro-MMP-1의 발현량을 감소시키는 효과가 있다는 것을 확인하였다.As a result, as shown in FIG. 3, both the Omiza fruit and leaf extract had an effect of reducing the expression amount of Pro-MMP-1 increased by TNF-α, and in particular, the Omiza leaf extract was lower MMP-1 &lt; SEP &gt;

Claims (7)

  1. 오미자 추출물을 유효성분으로 포함하는 피부 주름 개선용 화장료 조성물.A cosmetic composition for improving skin wrinkles comprising an extract of Omija as an active ingredient.
  2. 제1항에 있어서, 상기 오미자 추출물은 오미자의 잎, 줄기 또는 이들의 혼합 추출물인 것을 특징으로 하는 피부 주름 개선용 화장료 조성물.The cosmetic composition for improving skin wrinkles according to claim 1, wherein the Schizandra chinensis extract is a leaf, a stem or a mixed extract thereof.
  3. 제1항에 있어서, 상기 오미자 추출물의 추출용매는 물, C1~C4의 저급 알코올 또는 이들의 혼합물인 것을 특징으로 하는 피부 주름 개선용 화장료 조성물.The method of claim 1, wherein the extraction solvent of the Schisandra chinensis extract is water, C 1 ~ C 4 lower alcohol or a cosmetic composition for improving skin wrinkles, characterized in that a mixture thereof.
  4. 제1항에 있어서, 상기 오미지 추출물은 콜라게나아제 저해 활성을 갖는 것을 특징으로 하는 피부 주름 개선용 화장료 조성물.The cosmetic composition for improving skin wrinkles according to claim 1, wherein the extract has an activity of inhibiting collagenase.
  5. 제1항에 있어서, 상기 화장료 조성물은 피부 외용 연고, 크림, 유연화장수, 영양화장수, 팩, 에센스, 헤어토닉, 샴푸, 린스, 헤어 컨디셔너, 헤어 트리트먼트, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처 로션, 영양로션, 마사지 크림, 영양크림, 모이스처 크림, 핸드 크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 바디 로션 및 바디 클렌저로 이루어지는 군으로부터 선택된 어느 하나의 제형인 것을 특징으로 하는 피부 주름 개선용 화장료 조성물.The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Wherein the composition is at least one selected from the group consisting of:
  6. 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 건강기능식품 조성물.A health functional food composition for preventing or improving skin wrinkles containing an extract of Omija as an active ingredient.
  7. 오미자 추출물을 유효성분으로 포함하는 피부 주름의 예방 또는 개선용 의약외품 조성물.A quasi-drug composition for prevention or improvement of skin wrinkles containing an extract of Omija as an active ingredient.
PCT/KR2018/015746 2017-12-22 2018-12-12 Skin wrinkle prevention and improvement composition containing schisandra chinensis extract as active ingredient WO2019124862A1 (en)

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