KR102379698B1 - A composition for skin whitening or regeneration comprising Melandrium firmum extract - Google Patents
A composition for skin whitening or regeneration comprising Melandrium firmum extract Download PDFInfo
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- KR102379698B1 KR102379698B1 KR1020150082862A KR20150082862A KR102379698B1 KR 102379698 B1 KR102379698 B1 KR 102379698B1 KR 1020150082862 A KR1020150082862 A KR 1020150082862A KR 20150082862 A KR20150082862 A KR 20150082862A KR 102379698 B1 KR102379698 B1 KR 102379698B1
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- extract
- skin
- melandrium
- skin whitening
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- A—HUMAN NECESSITIES
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- Cosmetics (AREA)
Abstract
본 발명은 왕불유행 추출물(Melandrium firmum)을 유효성분으로 포함하는 피부 미백 또는 재생용 조성물 및 이를 이용한 방법에 관한 것이다. 구체적으로, 본 발명의 왕불유행 추출물을 포함하는 조성물은 멜라닌 합성 억제 및 색소 침착 저해 활성을 통해 피부 미백에 탁월한 효과를 보이며, 자유라디칼 소거 및 세포증식 촉진 활성을 통해 피부 재생에 우수한 효과를 보이고, 또한 인체에 적용하여도 부작용을 유발하지 않으므로 화장료 조성물, 약학적 조성물, 건강기능식품 조성물 또는 의약외품 조성물로 활용도가 높다. The present invention extracts Wangbulbyeong ( Melandrium ) firmum ) as an active ingredient, and relates to a composition for skin whitening or regeneration, and a method using the same. Specifically, the composition containing the extract of the present invention shows an excellent effect on skin whitening through melanin synthesis inhibition and pigmentation inhibitory activity, and shows an excellent effect on skin regeneration through free radical scavenging and cell proliferation promoting activity, In addition, since it does not cause side effects even when applied to the human body, it has high utility as a cosmetic composition, a pharmaceutical composition, a health functional food composition, or a quasi-drug composition.
Description
본 발명은 왕불유행 추출물을 포함하는 피부 미백 또는 재생용 조성물 및 이를 이용한 방법에 관한 것이다.
The present invention relates to a composition for skin whitening or regenerating comprising an extract of Wangbulbyeonghyeong and a method using the same.
피부는 외부환경에 대한 방어벽 역할을 하여 화학물질이나 자외선을 포함한 외부 환경오염 물질 또는 미생물의 침입을 방어함으로써 주위환경으로부터 생체를 보호한다.
The skin acts as a barrier against the external environment and protects the living body from the surrounding environment by preventing the intrusion of external environmental pollutants or microorganisms including chemicals and ultraviolet rays.
사람의 피부색은 멜라닌, 카로틴, 헤모글로빈의 양에 따라 결정되며, 그 중에서도 멜라닌이 가장 결정적인 요소로 작용한다. 검은 색소와 단백질의 복합체 형태를 갖는 페놀계 고분자 물질인 멜라닌 색소는 자외선 차단의 역할을 수행한다. 따라서, 멜라닌 색소가 부족한 사람은 햇빛에 매우 민감하여 화상을 입기 쉬우며, 피부암의 발생 확률이 높아지게 된다. 단파장의 자외선 및 발암물질은 피부에서 유해한 자유라디칼을 형성하는데, 멜라닌은 이러한 자유라디칼 등을 제거하여 단백질과 유전자를 보호해 주는 유용한 역할을 한다. 따라서, 멜라닌의 양이 많다는 것은 물리적 또는 화학적 독성 물질로부터 피부를 보호할 수 있는 효과적인 대응 체계를 가지고 있다는 것을 의미한다. A person's skin color is determined by the amounts of melanin, carotene, and hemoglobin, and among them, melanin acts as the most decisive factor. Melanin pigment, which is a phenol-based polymer having a complex form of black pigment and protein, plays a role of blocking ultraviolet rays. Therefore, a person lacking melanin pigment is very sensitive to sunlight and is easily burned, and the probability of skin cancer increases. Short-wavelength ultraviolet rays and carcinogens form harmful free radicals in the skin, and melanin plays a useful role in protecting proteins and genes by removing these free radicals. Therefore, having a large amount of melanin means having an effective countermeasure system that can protect the skin from physical or chemical toxic substances.
멜라닌은 색소 세포 내에 존재하는 티로시나아제(tyrosinase)의 작용에 의해 티로신으로부터 복잡한 과정을 거쳐 생성된다. 생성된 멜라닌은 피부 세포에 전달되고, 표피 박리와 함께 멜라닌이 상실되어 소멸되는 순환 작용을 보인다. 즉, 피부가 자외선에 노출되면 티로시나아제가 활성화되는데, 상기 티로시나아제가 피부조직에 존재하는 티로신에 작용하여 도파(DOPA) 및 도파퀴논을 생성시키는 산화 과정을 유도하고, 이로 인해 피부 색소 세포인 멜라노사이트 내의 멜라노좀에서 멜라닌이 합성되며, 합성된 멜라닌은 피부의 각질 형성 세포인 케라티노사이트로 전달되고, 각질화 과정에 의해 피부 표면에 도달하여 자외선으로부터 피부를 보호하게 된다. 그러나, 멜라닌이 국소적으로 과도하게 합성되거나, 피부 병변 및 노화에 따라 피부의 생리 기능이 떨어지게 되면, 멜라닌이 피부 표면에 침착되어 기미, 주근깨 등의 다양한 색소 침착을 유발하게 된다.
Melanin is produced from tyrosine through a complex process by the action of tyrosinase present in pigment cells. The generated melanin is delivered to the skin cells, and the melanin is lost and disappears along with epidermal peeling, showing a circulatory action. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated, and the tyrosinase acts on tyrosine present in the skin tissue to induce an oxidation process to generate DOPA and dopaquinone, thereby inducing skin pigment cells In melanocytes, melanin is synthesized in melanosomes, and the synthesized melanin is transferred to keratinocytes, which are keratinocytes of the skin, and reaches the skin surface through keratinization to protect the skin from UV rays. However, when melanin is locally excessively synthesized or the physiological function of the skin is deteriorated due to skin lesions and aging, melanin is deposited on the skin surface to cause various pigmentation such as spots and freckles.
상기에 기재한 바와 같이 피부 색소 침착(흑화)의 원인과 기작이 밝혀지면서, 피부에서의 멜라닌 색소침착 방지는 주로 하기의 네 가지 관점에서 연구되어 왔다:As the causes and mechanisms of skin pigmentation (blackening) have been elucidated as described above, the prevention of melanin pigmentation in the skin has been mainly studied from the following four perspectives:
1) 멜라닌 합성의 주효소인 티로시나아제 활성을 조절하기 위하여 티로시나아제 합성 저해 물질이나 티로시나아제의 기질에 대한 길항물질 개발;1) Development of tyrosinase synthesis inhibitors or antagonists of tyrosinase substrates in order to regulate the activity of tyrosinase, the main enzyme of melanin synthesis;
2) 동물의 멜라닌 생합성 장소인 멜라노사이트의 기능을 저하시키기 위해 멜라노사이트에 독성을 나타내는 물질 개발;2) Development of substances that are toxic to melanocytes in order to decrease the function of melanocytes, the site of melanin biosynthesis in animals;
3) 멜라닌 합성 경로의 중간 대사물질인 도파(DOPA)의 산화방지를 위해 도파의 환원물질 개발;3) Development of a reducing substance of dopa to prevent oxidation of DOPA, an intermediate metabolite of the melanin synthesis pathway;
4) 멜라닌 생성 기구인 제1효소 티로시나아제, 도파크롬(DOPA chrome)으로부터 DHICA(5-6,디히드록시인돌-2-카복실산)로의 변환을 촉매하는 제2효소 도파크롬 타우토머라아제(DOPA chrome tautomerase), 및 DHICA로부터 인돌-5,6-퀴논-2-카르복실산(indole-5,6-quinone-2-carboxylic acid)으로의 변환을 촉매하는 제3효소의 활성을 동시에 감소시키는 방법 개발.
4) The first enzyme tyrosinase, which is a melanin production mechanism, and the second enzyme dopachrome tautomerase, which catalyzes the conversion from dopachrome (DOPA chrome) to DHICA (5-6, dihydroxyindole-2-carboxylic acid) ( DOPA chrome tautomerase), and simultaneously reducing the activity of a third enzyme that catalyzes the conversion from DHICA to indole-5,6-quinone-2-carboxylic acid method development.
한편, 피부 색소가 침착되는 것을 방지하거나, 또는 개선하기 위해 사용하고 있는 대표적인 물질에는 티로시아나제 효소 활성을 억제하는 물질인 코지산(kojic acid), 알부틴(arbutin), 하이드로퀴논(hydroquinone), 비타민 C(L-ascorbic acid) 및 이들의 유도체 또는 각종 식물 추출물들이 있다. 그러나, 상기 물질들은 처방계 중에서 분해되어 착색되거나, 이취의 발생, 생체 레벨에서의 효능·효과의 불분명 또는 안전성 문제 등으로 그 사용이 제한되고 있는 실정이다. On the other hand, representative substances used to prevent or improve skin pigmentation include kojic acid, arbutin, hydroquinone, which are substances that inhibit tyrosianase enzyme activity, There are vitamin C (L-ascorbic acid) and derivatives thereof or various plant extracts. However, these substances are decomposed and colored in the prescription system, and their use is limited due to the occurrence of off-flavor, unclear efficacy/effect at the biological level, or safety issues.
코지산은 티로시나아제의 활성 부위에 존재하는 구리 이온을 흡착시켜 효소활성을 저해하는데, 화장품에 배합 시 불안정성, 피부 부작용 등의 문제들을 야기하고, 최근 동물실험 결과에서 간암을 유발한다고 밝혀져, 화장품 원료로서의 사용이 중지되었다. 비타민 C 및 그 유도체는 산화가 잘되는 불안정성 때문에 화장품 원료로 사용이 어려우며, 하이드로퀴논은 피부에 대한 미백효과는 탁월하지만 알레르기 유발 특성, 멜라닌 생성 세포에 대한 독성, 피부의 영구 탈색화 등 피부에 대한 자극성이 높다. 또한, 최근에는 발암성 물질로 규정되어 각 나라별로 제한적인 농도만 허가하고 있다. 알부틴은 하이드로퀴논에 글루코피라노사이드(glucopyranoside)가 결합된 유도체이며, 하이드로퀴논의 사용 시 나타나는 부작용이 적으면서 인체에 대한 독성이 없고, 멜라닌 색소의 합성을 억제하는 작용이 있어 멜라닌 색소 침착이 증가하는 피부 질환의 치료제로서의 이용 가능성이 제시되었으나, 그 역시 피부 효소에 의해 일부 분해되는 단점이 있다. 따라서, 소량으로도 효능이 우수하며, 부작용이 적은 안전한 대체 미백제의 개발이 시급한 실정이다.
Kojic acid inhibits enzyme activity by adsorbing copper ions present at the active site of tyrosinase, causing problems such as instability and skin side effects when formulated in cosmetics. has been discontinued as a Vitamin C and its derivatives are difficult to use as cosmetic raw materials due to their instability due to their oxidation well, and hydroquinone has excellent skin whitening effect, but has allergenic properties, toxicity to melanocytes, and permanent depigmentation of the skin. this is high In addition, recently, it has been regulated as a carcinogen and only limited concentrations are permitted in each country. Arbutin is a derivative in which glucopyranoside is combined with hydroquinone. It has few side effects when using hydroquinone and is non-toxic to the human body. Possibility of use as a therapeutic agent for skin diseases has been suggested, but it also has a disadvantage in that it is partially decomposed by skin enzymes. Therefore, there is an urgent need to develop a safe alternative whitening agent that is effective even in a small amount and has few side effects.
이러한 배경하에서, 본 발명자들은 피부의 미백 또는 재생을 개선시키거나, 또는 향상시키는 효과를 가진 물질을 개발하기 위해 예의 노력한 결과, 왕불유행 추출물이 멜라닌 합성을 효과적으로 억제할 뿐 아니라 자유라디칼 소거, 세포증식 촉진에 우수한 효과가 있음을 규명하여, 이를 활용한 피부의 미백 또는 재생용 화장료 조성물을 완성하였다.
Under this background, the present inventors have made diligent efforts to develop a substance having an effect of improving or improving skin whitening or regeneration, and as a result, the extract of Wangbulbyeonghyeong effectively inhibits melanin synthesis as well as free radical scavenging, cell proliferation It was found that it has an excellent effect on promotion, and a cosmetic composition for skin whitening or regeneration using this was completed.
본 발명의 목적은 왕불유행 추출물(Melandrium firmum)을 유효성분으로 포함하는 피부 미백 또는 재생용 조성물을 제공하는 것이다.An object of the present invention is to extract Wangbulbyeong ( Melandrium ) firmum ) as an active ingredient to provide a composition for skin whitening or regeneration.
본 발명의 다른 목적은 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 화장료 조성물을 제공하는 것이다.Another object of the present invention is to provide a cosmetic composition for skin whitening or regenerating comprising the extract of Wangbulbyeonghyeong as an active ingredient.
본 발명의 또 다른 목적은 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 약학적 조성물을 제공하는 것이다.Another object of the present invention is to provide a pharmaceutical composition for skin whitening or regenerating comprising the extract of Wangbulbyeongheung as an active ingredient.
본 발명의 또 다른 목적은 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 건강기능식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a health functional food composition for skin whitening or regenerating comprising the extract of Wangbulbyeonghyeong as an active ingredient.
본 발명의 또 다른 목적은 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 의약외품 조성물을 제공하는 것이다.Another object of the present invention is to provide a quasi-drug composition for skin whitening or regenerating comprising an extract of Wangbulbyeongheung as an active ingredient.
본 발명의 또 다른 목적은 왕불유행 추출물을 유효성분으로 포함하는 조성물을 개체에 투여하는 단계를 포함하는 피부 미백 또는 재생 방법을 제공하는 것이다.
Another object of the present invention is to provide a skin whitening or regenerating method comprising the step of administering to an individual a composition comprising an extract of Wangbulbyeongheung as an active ingredient.
상기 목적을 해결하기 위한 하나의 양태로서, 본 발명은 왕불유행 추출물을 유효성분으로 함유하는 피부 미백 또는 재생용 조성물을 제공한다.As one aspect for solving the above object, the present invention provides a composition for skin whitening or regenerating containing the extract of Wangbulbyeongheung as an active ingredient.
상기 조성물은 화장료, 약학적, 건강기능식품 또는 의약외품 조성물일 수 있다.
The composition may be a cosmetic, pharmaceutical, health functional food or quasi-drug composition.
왕불유행(Melandrium firmum)은 패랭이꽃과(Caryophyllacae)에 속하는 이년생 초본인 장구채(Melandrium firmum) 및 애기장구채(Melandrium apricum)의 종자로서, 활혈, 통경, 최유 등의 효능이 있어 물월경, 유즙불통, 금창출혈 등의 치료를 위해 사용되는 것으로 알려져 있다. 하지만, 피부의 미백 또는 재생과 관련된 효과는 알려진 바 없으며, 사람 피부에 대한 연구도 충분히 이루어지지 않고 있다.
Melandrium firmum is a biennial herb belonging to the family Caryophyllacae , Melandrium . firmum ) and Arabidopsis ( Melandrium apricum ) seeds, which are known to be used for the treatment of water menstruation, infertility of milk, and bleeding from geumchang because they have effects such as hwalyeol, tuberculosis, and aphrodisiac. However, effects related to skin whitening or regeneration are not known, and studies on human skin have not been sufficiently conducted.
본 발명의 용어, "추출물"은 어떤 물질을 용매에 녹여 분리한 물질을 의미하고, 구체적으로 상기 본발명의 왕불유행의 추출처리에 의하여 얻어지는 추출액, 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등의 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다.As used herein, the term "extract" refers to a substance separated by dissolving a certain substance in a solvent, specifically, an extract obtained by the extraction treatment of the present invention, a diluted or concentrated solution of the extract, and obtained by drying the extract It includes extracts of all formulations that can be formed using the extract itself and the extract, such as a dried product, a prepared product or purified product of the extract, or a mixture thereof.
상기 본 발명의 추출물은 식물의 천연, 잡종 또는 변종 식물로부터 추출될 수 있고, 식물 조직 배양물로부터도 추출될 수 있다.The extract of the present invention may be extracted from natural, hybrid or mutated plants of plants, and may also be extracted from plant tissue culture.
상기 본 발명의 왕불유행을 추출하는 방법은 특별히 제한되지 않으며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 추출할 수 있다. 상기 추출 방법의 비제한적인 예로는, 열수 추출법, 초음파 추출법, 여과법, 환류 추출법 등을 들 수 있으며, 이들은 단독으로 수행되거나 2 종 이상의 방법을 병용하여 수행될 수 있다.The method of extracting the wangbulbyeong of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, and reflux extraction, and these may be performed alone or in combination of two or more methods.
상기 본 발명의 왕불유행을 추출하는데 사용되는 용매의 종류는 특별히 제한되지 않으며, 당해 기술 분야에서 공지된 임의의 용매를 사용할 수 있다. 상기 추출 용매의 비제한적인 예로는 물; 메탄올, 에탄올, 프로필알코올, 부틸알코올 등의 C1 내지 C4의 저급 알코올; 글리세린, 부틸렌글리콜, 프로필렌글리콜 등의 다가 알코올; 및 메틸아세테이트, 에틸아세테이트, 아세톤, 벤젠, 헥산, 디에틸에테르, 디클로로메탄 등의 탄화수소계 용매; 또는 이들의 혼합물을 사용할 수 있으며, 구체적으로 물, 저급알코올, 1,3-부틸렌글리콜, 에틸아세테이트를 단독으로 사용하거나 2종 이상 혼합하여 사용할 수 있다. 더욱 구체적으로 본 발명에서 왕불유행을 추출하는 용매는 물(또는 증류수)을 사용할 수 있다. 상기 용매를 사용하여 왕불유행을 1회 이상 추출하여 용매 추출물을 제조할 수 있으며, 상기 용매 추출물을 감압 증류한 후 동결건조 또는 분무 건조하여 얻은 건조 추출물을 제조할 수 있다. 본 발명의 일 실시예에서는 추출용매로 물을 사용하여 왕불유행 추출물을 제조하였다(실시예 1).The type of solvent used for extracting Wangbulbyeong of the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C 1 to C 4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. More specifically, in the present invention, water (or distilled water) may be used as a solvent for extracting Wangbulbyeonghyeong. A solvent extract may be prepared by extracting Wangbulbyeongheung one or more times using the solvent, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying may be prepared. In an embodiment of the present invention, using water as an extraction solvent, an extract of Wangbulbyeonghyeong was prepared (Example 1).
상기 본 발명에서 열수 추출한 추출물은 부유하는 고체 입자를 제거하기 위해 여과, 예를 들어 나일론 등을 이용해 입자를 걸러내거나 냉동여과법 등을 이용해 여과한 후, 그대로 사용하거나 이를 동결건조, 열풍건조, 분무건조 등을 이용해 건조시켜 사용할 수도 있다.The extract extracted with hot water in the present invention is filtered to remove floating solid particles, for example, filtered using nylon or the like, or filtered using freeze filtration, etc. It can also be used after drying.
본 발명의 용어 "미백"은, 피부를 하얗게 하는 것을 의미하며, 멜라닌의 과도한 합성이나, 멜라닌 침착으로 인한 기미, 주근깨를 포함한 다양한 색소 침착을 완화 또는 개선하는 것을 의미한다. As used herein, the term "whitening" refers to whitening the skin, and means to alleviate or improve various pigmentation including freckles and freckles due to excessive synthesis of melanin or melanin deposition.
본 발명의 일 실시예에서는 상기 본 발명의 왕불유행 추출물의 피부 미백 효과를 확인하기 위하여 마우스 멜라노마 세포를 사용하여 세포 수준에서의 멜라닌 합성 억제 효과를 분석하였으며, 그 결과, 종래에 사용되고 있는 미백제(알부틴)보다 월등히 우수하게 멜라닌 생성 억제능(표 1)을 나타내는 것을 확인하였다. 또한, 왕불유행 추출물을 포함하는 피부외용제 또는 에센스를 성인 남녀 20명에게 도포하여 색소 침착 개선 여부를 확인하기 위한 관능 평가를 실시하였고, 왕불유행 추출물이 기미 또는 주근깨 등의 색소 침착을 개선하는데 우수한 효과를 나타낸다는 것을 확인하였다(표 6).
In one embodiment of the present invention, the effect of inhibiting melanin synthesis at the cellular level using mouse melanoma cells was analyzed to confirm the skin whitening effect of the extract of the present invention. arbutin) was significantly better than melanin production inhibitory ability (Table 1) was confirmed. In addition, a sensory evaluation was carried out to check whether pigmentation was improved by applying an external skin preparation or essence containing the extract of Wangbulvain extract to 20 adult men and women, and the extract was excellent in improving pigmentation such as spots or freckles It was confirmed that it represents (Table 6).
본 발명의 용어 "재생"은 피부 외부 및 내부 원인에 의해 발생된 피부(세포) 손상에 대한 피부 조직의 회복 과정을 의미한다. 상기 외부 원인에 의한 손상은 자외선, 외부 오염 물질, 창상, 외상 등이 원인일 수 있으며, 상기 내부 원인에 의한 손상은 스트레스 등이 원인일 수 있다. As used herein, the term “regeneration” refers to a process of repairing skin tissue against skin (cell) damage caused by external and internal causes of the skin. The damage caused by the external cause may be caused by ultraviolet rays, external pollutants, wounds, trauma, and the like, and the damage caused by the internal cause may be caused by stress.
본 발명의 일 실시예에서는 왕불유행 추출물의 피부 재생 효과를 확인하기 위하여 DPPH 방법을 통한 자유라디칼 소거능을 분석하고, 인체 섬유아세포를 배양하여 MTT 환원법으로 세포증식 촉진 활성을 분석하였다. 그 결과, 왕불유행 추출물은 자유라디칼 제거제로 사용되고 있는 아스코르브산 보다 우수한 자유라디칼 소거능을 나타내는 것을 확인하였고(표 2), 탁월한 세포증식 촉진 활성을 농도 의존적으로 나타내는 것을 확인하였다(표 3).
In one embodiment of the present invention, in order to confirm the skin regeneration effect of the extract of Wangbulbyeongheung, the free radical scavenging ability through the DPPH method was analyzed, and the cell proliferation promoting activity was analyzed by culturing human fibroblasts by the MTT reduction method. As a result, it was confirmed that the extract of Wangbulbyeonghyeong extract exhibited superior free radical scavenging activity than ascorbic acid, which is used as a free radical scavenger (Table 2), and it was confirmed that it exhibited excellent cell proliferation promoting activity in a concentration-dependent manner (Table 3).
상기 목적을 해결하기 위한 다른 하나의 양태로서, 본 발명은 왕불유행 추출물을 유효성분으로 함유하는 피부 미백 또는 재생용 화장료 조성물을 제공한다.As another aspect for solving the above object, the present invention provides a cosmetic composition for skin whitening or regenerating containing the extract of Wangbulbyeongheung as an active ingredient.
본 발명의 화장료 조성물은 총 조성물의 중량 대비 왕불유행 추출물을 0.0001 내지 50 중량%로 포함할 수 있으며, 구체적으로 0.01 중량% 내지 10 중량%로 포함할 수 있다. 상기 범위 내에서 우수한 피부 미백 또는 재생 효과를 나타내는 이점이 있으며, 조성물의 제형이 안정화되는 이점이 있다.The cosmetic composition of the present invention may include 0.0001 to 50% by weight of the extract of King Bullfly relative to the total weight of the composition, specifically 0.01 to 10% by weight. There is an advantage of exhibiting an excellent skin whitening or regenerating effect within the above range, and there is an advantage that the formulation of the composition is stabilized.
상기 본 발명의 화장료 조성물은 용액, 외용 연고, 크림, 폼, 영양 화장수, 유연 화장수, 팩, 유연수, 유액, 메이크업 베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린 크림, 선 오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면 활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패취 및 스프레이로 이루어진 군으로부터 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention includes a solution, an external ointment, a cream, a foam, a nourishing lotion, a flexible lotion, a pack, a soft water, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bath agent, a sunscreen cream, a sun oil, a suspension, Emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays can be prepared in a formulation selected from the group consisting of, but limited thereto it is not
상기 본 발명의 화장료 조성물은 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1종 이상 추가로 포함할 수 있으며, 통상의 성분으로 예를 들면 유분, 물, 계면 활성제, 보습제, 저급 알코올, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, and thickener. , a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but is not limited thereto.
상기 본 발명의 화장료 조성물에 포함되는 화장품학적으로 허용 가능한 담체는 화장료 조성물의 제형에 따라 다양하다.The cosmetically acceptable carrier included in the cosmetic composition of the present invention is various depending on the formulation of the cosmetic composition.
본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체 성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화 아연 등이 이용될 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.When the formulation of the present invention is an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be used, but is not limited thereto. These may be used alone or in combination of two or more.
본 발명의 제형이 파우더 또는 스프레이인 경우에는, 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록사이드, 칼슘 실케이트, 폴리아미드 파우더 등이 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로하드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진제를 포함할 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohard propellants such as, but not limited to, locarbon, propane/butane or dimethyl ether. These may be used alone or in combination of two or more.
본 발명의 제형이 용액 또는 유탁액인 경우에는, 담체 성분으로서 용매, 용해화제 또는 유탁화제 등이 이용될 수 있으며, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜 오일 등이 이용될 수 있고, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브 오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like can be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, fatty acid ester of polyethylene glycol or sorbitan may be used, but is not limited thereto. These may be used alone or in combination of two or more.
본 발명의 제형이 현탁액인 경우에는, 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타하이드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.
본 발명의 제형이 비누인 경우에는, 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알코올, 식물성 유, 글리세롤, 당 등이 이용될 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. are used as carrier components may be, but is not limited thereto. These may be used alone or in combination of two or more.
본 발명의 제형이 계면-활성제 함유 클렌징인 경우에는, 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시테이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 오일, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있으나, 이에 제한되는 것은 아니다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.
When the formulation of the present invention is a surfactant-containing cleansing agent, fatty alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcositate, fatty acid as carrier components Amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used, but is not limited thereto. These may be used alone or in combination of two or more.
상기 목적을 해결하기 위한 또 다른 하나의 양태로서, 본 발명은 왕불유행 추출물을 유효성분으로 함유하는 피부 미백 또는 재생용 약학적 조성물을 제공한다.As another aspect for solving the above object, the present invention provides a pharmaceutical composition for skin whitening or regenerating containing the extract of Wangbulbyeongheung as an active ingredient.
본 발명의 약학적 조성물은 총 조성물의 중량 대비 왕불유행 추출물을 0.0001 내지 50 중량%로 포함할 수 있으며, 구체적으로 0.01 중량% 내지 10 중량%로 포함할 수 있다. 상기 범위 내에서 우수한 피부 미백 또는 재생 효과를 나타내는 이점이 있으며, 조성물의 제형이 안정화되는 이점이 있다.The pharmaceutical composition of the present invention may include 0.0001 to 50% by weight of the extract of Wangbulbyeongheung relative to the total weight of the composition, specifically, it may include 0.01 to 10% by weight. There is an advantage of exhibiting an excellent skin whitening or regenerating effect within the above range, and there is an advantage that the formulation of the composition is stabilized.
상기 약학적 조성물은 정제, 환제, 산제, 과립제, 캡슐제, 현탁제, 내용액제, 유제, 시럽제, 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결 건조제 및 좌제으로 이루어진 군으로부터 선택되는 어느 하나의 제형을 가질 수 있으며, 경구 또는 비경구의 여러 가지 제형일 수 있다.The pharmaceutical composition is any one selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, internal solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-drying agents and suppositories It may have a dosage form, and may be oral or parenteral various dosage forms.
제제화할 경우에는 통상적으로 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다. In the case of formulation, it can be prepared using a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, and a surfactant commonly used.
본 발명의 제형이 경구투여를 위한 고형제제인 경우에는, 정제, 환제, 산제, 과립제, 캡슐제 등이 포함될 수 있으며, 상기 고형제제는 하나 이상의 화합물에 적어도 하나 이상의 부형제, 예를 들면 전분, 탄산칼슘, 수크로오스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제될 수 있다. 또한, 단순한 부형제 이외에 스테아린산 마그네슘, 탈크 등과 같은 윤활제들이 사용될 수 있다. When the dosage form of the present invention is a solid preparation for oral administration, tablets, pills, powders, granules, capsules, etc. may be included, and the solid preparation includes one or more compounds and at least one excipient, for example, starch, carbonic acid. It may be prepared by mixing calcium, sucrose or lactose, gelatin, or the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may be used.
본 발명의 제형이 경구투여를 위한 액상제제인 경우에는, 현탁제, 내용액제, 유제, 시럽제 등이 포함될 수 있으며, 통상적으로 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. When the formulation of the present invention is a liquid formulation for oral administration, suspensions, internal solutions, emulsions, syrups, etc. may be included, and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients, for example, wetting agents , sweeteners, fragrances, preservatives, and the like may be included.
본 발명의 제형이 비경구투여를 위한 제제인 경우에는, 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함될 수 있다. 상기 비수성용제, 현탁용제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있으며, 상기 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로젤라틴 등이 사용될 수 있다.
When the formulation of the present invention is a formulation for parenteral administration, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories may be included. As the non-aqueous solvent and suspension solution, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, etc. may be used, and the base of the suppository is witepsol , macrogol, tween 61, cacao butter, laurin, glycerogelatin, etc. may be used.
상기 목적을 해결하기 위한 또 다른 하나의 양태로서, 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 건강기능식품 조성물을 제공한다.As another aspect for solving the above object, there is provided a health functional food composition for skin whitening or regeneration comprising the extract of King Bullfinch as an active ingredient.
상기 건강기능식품 조성물은 육류, 소세지, 빵, 쵸코렛, 캔디류, 스넥류, 과자류, 피자, 라면, 껌류, 아이스크림류, 스프, 음료수, 차, 기능수, 드링크제, 알콜 음료 및 비타민 복합제 중 어느 하나의 형태일 수 있다.The health functional food composition is in the form of any one of meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcoholic beverage, and vitamin complex can be
또한, 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있으며, 그 밖에 천연 과일주스 및 과일주스 음료 및 야채음료의 제조를 위한 과육을 함유할 수 있다. 이들은 단독으로 사용되거나 2종 이상 혼합되어 사용될 수 있다.In addition, various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective It may contain a sexual colloid thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonation agent used in carbonated beverages, etc., and may contain other natural fruit juices and pulp for the production of fruit juice beverages and vegetable beverages. can These may be used alone or in combination of two or more.
상기 건강기능식품 조성물은 식품첨가물을 추가로 포함할 수 있으며, "식품첨가물"로서의 적합 여부는 다른 규정이 없는 한 식품의약품안정처에 승인된 식품첨가물공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의해 판정한다.The health functional food composition may additionally contain food additives, and whether or not it is suitable as a "food additive" is determined according to the general rules and general test methods of the Food Additives Code approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the relevant standards and standards.
상기 "식품첨가물공전"에 수재된 품목으로 예를 들어, 케톤류, 글리신, 구연산칼륨, 니코틴산, 계피산 등의 화학적 합성품, 감색소, 감초추출물, 결정셀롤로오스, 고랭색소, 구아검 등의 천연첨가물, L-글루타민산나트륨제제, 면류첨가알칼리제, 보존료제제, 타르색소제제 등의 혼합 제제류들을 들 수 있다.Items listed in the "Food Additives Code", for example, chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as depigmentation, licorice extract, crystalline cellulose, high-cold pigment, and guar gum , L-sodium glutamate preparations, noodle-added alkalis, preservatives, and mixed preparations such as tar color preparations.
이때, 건강기능식품을 제조하는 과정에서 음료를 포함한 식품에 첨가되는 본 발명에 따른 추출물은 필요에 따라 그 함량을 적절히 가감할 수 있으며, 구체적으로 식품 100중량%에 0.01 내지 10 중량% 포함되도록 첨가할 수 있다.
At this time, the extract according to the present invention, which is added to food including beverages in the process of manufacturing health functional food, may appropriately increase or decrease its content as needed, and specifically, it is added so as to include 0.01 to 10% by weight in 100% by weight of food. can do.
상기 목적을 해결하기 위한 또 다른 하나의 양태로서, 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 의약외품 조성물을 제공한다.As yet another aspect for solving the above object, there is provided a quasi-drug composition for skin whitening or regeneration comprising the extract of King Bullfinch as an active ingredient.
상기 본 발명의 의약외품 조성물은 바디 클렌저, 소독 청결제, 세정제, 주방용 세정제, 청소용 세정제, 치약, 가글제, 물티슈, 세제, 비누, 핸드 워시, 헤어세정제, 헤어 유연제, 가습기 충진제, 마스크, 연고제 및 필터 충진제로 이루어진 군에서 선택되는 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.
The quasi-drug composition of the present invention is a body cleanser, disinfectant cleaner, detergent, kitchen cleaner, cleaning agent, toothpaste, gargle, wet tissue, detergent, soap, hand wash, hair cleaner, hair softener, humidifier filler, mask, ointment and filter filler. It may be prepared in a formulation selected from the group consisting of, but is not limited thereto.
상기 목적을 해결하기 위한 또 다른 하나의 양태로서, 왕불유행 추출물을 유효성분으로 포함하는 조성물을 개체에 투여하는 단계를 포함하는 피부 미백 또는 재생 방법을 제공한다.As another aspect for solving the above object, there is provided a skin whitening or regenerating method comprising administering to an individual a composition comprising an extract of King Bullfinch as an active ingredient.
본 발명의 용어, "개체"는 피부의 미백 또는 재생의 효과를 필요로하는 인간을 포함한 모든 동물을 의미하며, 본 발명의 용어, “투여”는 어떠한 적절한 방법으로 피부 미백 또는 재생을 위해 개체에 본 발명의 약학적 조성물을 도입하는 것을 의미한다. 상기 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있다. 구체적으로, 국소도포 등을 통한 경피투여 방식으로 투여될 수 있으나, 이에 제한되지 않는다.As used herein, the term "subject" refers to all animals, including humans, in need of skin whitening or regeneration. It means introducing the pharmaceutical composition of the present invention. The administration route of the composition may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue. Specifically, it may be administered by transdermal administration through topical application, etc., but is not limited thereto.
본 발명의 피부 미백 또는 재생 방법은 왕불유행 추출물, 또는 이의 약학적으로 허용 가능한 염을 포함하는 약학적 조성물을 약학적 유효량으로 투여하는 것을 포함할 수 있다. 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있으며, 본 발명의 목적상, 특정 환자에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 경우에 따라 다른 제제가 사용되는지의 여부를 비롯한 구체적 조성물, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여 시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
The skin whitening or regenerating method of the present invention may include administering a pharmaceutical composition comprising an extract of Wangbulinaceae or a pharmaceutically acceptable salt thereof in a pharmaceutically effective amount. A suitable total daily amount may be determined by a treating physician within the scope of sound medical judgment, and for the purposes of the present invention, a specific therapeutically effective amount for a specific patient may vary depending on the type and degree of response to be achieved, and in some cases, other agents The specific composition including whether or not it is used, the patient's age, weight, general health status, sex and diet, administration time, administration route and rate of secretion of the composition, duration of treatment, drugs used or concurrently with the specific composition, etc. It is preferable to apply differently depending on factors and similar factors well known in the medical field.
본 발명의 왕불유행 추출물을 포함하는 조성물은 피부 미백 또는 재생 효능을 갖는다. 구체적으로 본 발명의 조성물은 멜라닌 합성을 억제하고, 색소 침착을 저해하며, 자유라디칼을 소거하고, 세포증식을 촉진시키는 효능이 우수하다.The composition comprising the extract of the present invention has a skin whitening or regenerating effect. Specifically, the composition of the present invention is excellent in inhibiting melanin synthesis, inhibiting pigmentation, scavenging free radicals, and promoting cell proliferation.
따라서, 천연물 유래의 물질인 본 발명의 조성물은 인체에 적용하여도 부작용을 유발하지 않아 화장료 조성물, 약학적 조성물, 건강기능식품 조성물 또는 의약외품 조성물로 유용하게 사용할 수 있다.
Therefore, the composition of the present invention, which is a material derived from a natural product, does not cause side effects even when applied to the human body, and thus can be usefully used as a cosmetic composition, a pharmaceutical composition, a health functional food composition, or a quasi-drug composition.
이하, 실시예를 통하여 본 발명의 구성 및 효과를 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것일 뿐 본 발명의 범위가 이들 실시예에 의해 제한되는 것은 아니다.
Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.
실시예Example 1: One: 왕불유행royal fashion 추출물의 제조 Preparation of extracts
구입한 왕불유행 50g에 증류수 1L를 넣고 4시간 동안 끓인 다음 여과하고, 이를 건조하여 파우더 8.5g을 얻었다.
1L of distilled water was added to 50 g of the purchased Wangbulbyeong, boiled for 4 hours, filtered, and dried to obtain 8.5 g of powder.
실시예Example 2: 멜라닌 생성 억제 시험 2: Melanin production inhibition test
세포 수준에서 왕불유행 추출물의 미백 효과를 확인하기 위해, 상기 획득한 왕불유행 추출물을 마우스의 멜라노마 세포(B-16) 배양액에 첨가하여 멜라닌 생성 억제 효과를 확인하였다.In order to confirm the whitening effect of the extract of Wangbulvain at the cellular level, the melanin production inhibitory effect was confirmed by adding the obtained extract of Wangbulinvain to the culture medium of melanoma cells (B-16) of the mouse.
최종농도 0.1 ㎍/㎖, 1 ㎍/㎖ 또는 10 ㎍/㎖의 왕불유행 추출물을 B-16 멜라노마 세포의 배양액에 각각 첨가하여 3일간 배양하였다. 이후, 세포들을 트립신(trypsin) 처리하여 배양용기로부터 떼어내 원심분리하고, 멜라닌을 추출하였다. 추출한 멜라닌에 수산화나트륨 용액(1N) 1 ㎖을 가한 후, 10분 동안 끓여 멜라닌을 용해하였고, 분광광도계를 이용하여 400 nm에서 용해물의 흡광도를 측정하였다. 생성된 멜라닌 양은 단위 세포수(106 세포) 당 흡광도로 나타내는 방법으로 측정하였다. 이때, 대조군으로는, 왕불유행 추출물을 첨가하지 않은 음성대조군과 음성대조군에 200 mg/㎖의 알부틴(arbutin)을 첨가한 양성대조군, 두 가지를 사용하였다. The final concentration of 0.1 ㎍ / ㎖, 1 ㎍ / ㎖ or 10 ㎍ / ㎖ Wangbulbyeongpyeonghwa extract was added to the culture medium of B-16 melanoma cells, respectively, and cultured for 3 days. Then, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. After adding 1 ml of sodium hydroxide solution (1N) to the extracted melanin, it was boiled for 10 minutes to dissolve the melanin, and the absorbance of the lysate was measured at 400 nm using a spectrophotometer. The amount of melanin produced was measured by a method expressed as absorbance per unit cell number (10 6 cells). At this time, as a control group, two types were used: a negative control group that did not contain the extract of Wangbulbyeongheung, and a positive control group that added 200 mg/ml of arbutin to the negative control group.
왕불유행 추출물을 첨가한 세포의 멜라닌 생성량은 음성대조군에 대한 저해율(%)로 계산하였고, 세 번의 실험을 반복하여 얻은 결과를 하기 표 1에 정리하였다.The amount of melanin production in the cells to which the extract of Wangbulbyeongheung was added was calculated as the inhibition rate (%) for the negative control group, and the results obtained by repeating the experiment three times are summarized in Table 1 below.
(음성대조군+알부틴(200 mg/㎖))positive control
(Negative control group + arbutin (200 mg/ml))
상기 표 1의 결과에서 보듯이, 왕불유행 추출물을 처리한 경우, 왕불유행 추출물을 처리하지 않은 음성대조군과 비교하여 최소 48% 이상의 멜라닌 생성 저해 효과를 발휘하였으며, 왕불유행 추출물은 일반적으로 미백 물질이라고 알려진 알부틴을 적용한 양성대조군보다 더 적은 농도로 월등히 우수한 멜라닌 생성 억제 효과가 있음을 확인하였다.
As can be seen from the results of Table 1, when the extract was treated with the extract, it exhibited an inhibitory effect on melanin production of at least 48% or more, compared to the negative control group that was not treated with the extract of the king and the queen, and the extract was generally said to be a whitening substance. It was confirmed that the known arbutin was applied at a lower concentration than the positive control group, and it was confirmed that there was a significantly superior melanin production inhibitory effect.
실시예Example 3: 피부 재생 효과 분석 3: Analysis of skin regeneration effect
실시예Example 3-1: 3-1: 자유라디칼free radical 소거능extinction ability 분석 analyze
피부 손상(피부 세포 재생 저하)을 유발하는 활성산소(자유라디칼) 생성에 대해 왕불유행 추출물이 미치는 영향을 확인하기 위하여, 라디칼 상태로 존재시 517 nm에서 최대 흡광을 보이며, 라디칼이 소거되면 흡광성을 잃는 특성이 있는 비교적 안정한 자유라디칼인 1,1-디페닐-2-피크릴히드라질(DPPH) 방법으로 자유라디칼 소거능을 측정하였다. DPPH(Sigma사에서 구입)는 0.15 mM의 농도로 메틸 알코올에 녹여 사용하였다. In order to confirm the effect of the extract of Wangbulbyeongpyeong on the generation of active oxygen (free radicals) that causes skin damage (decreased skin cell regeneration), it shows maximum absorption at 517 nm when it exists in a radical state, and absorbance when the radical is removed The free radical scavenging ability was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), which is a relatively stable free radical with the characteristic of losing . DPPH (purchased from Sigma) was dissolved in methyl alcohol at a concentration of 0.15 mM.
먼저, 왕불유행 추출물을 96-웰 플레이트의 각 웰에 100 ㎕씩 넣고, DPPH 용액 100 ㎕를 각각 첨가한 후, 상온에서 30분간 방치하였다. 이후, Micro plate reader(BiotTek EL-340)를 이용하여 517 nm에서 흡광도를 측정하였다. 이때, 대조군으로는 왕불유행 추출물을 첨가하지 않은 상태에서, 자유라디칼 제거제로 알려진 아스코르브산을 첨가한 대조군 1과 자유라디칼 소거능을 갖는 메틸 알코올을 첨가한 대조군 2, 두 가지를 사용하였다.First, 100 μl of the extract of Wangbulbyeongheung was put into each well of a 96-well plate, 100 μl of DPPH solution was added to each well, and then left at room temperature for 30 minutes. Then, the absorbance was measured at 517 nm using a micro plate reader (BiotTek EL-340). At this time, as a control, in the state in which the extract of Wangbulbyeonghyeong was not added, Control 1 to which ascorbic acid, known as a free radical scavenger, was added, and Control 2 to which methyl alcohol having a free radical scavenging ability was added were used.
결과는, 시료를 처리한 시험군의 흡광도가 대조군의 흡광도에 대해 절반 수준이 될 때의 왕불유행 추출물의 농도인 IC50(Inhibitory Concentration 50)으로 표시하였고, 하기 표 2에 정리하였다. The results were expressed as IC 50 (Inhibitory Concentration 50), which is the concentration of the extract of Wangbulbulgae when the absorbance of the test group treated with the sample was at a half level of the absorbance of the control group, and summarized in Table 2 below.
(아스코르브산)Control 1
(ascorbic acid)
(메틸 알코올) Control 2
(methyl alcohol)
상기 표 2의 결과에서 보듯이, 왕불유행 추출물을 처리한 경우, 아스코르브산을 처리한 대조군 1보다 현저히 우수한 자유라디칼 소거능을 보였으며, 또한, 메틸 알코올을 처리한 대조군 2와 비교하여도 강력한 자유라디칼을 소거 효과를 보인다는 것을 확인하였다.
As can be seen from the results of Table 2, when the extract of Wangbulbyeonghyeong was treated, it showed significantly better free radical scavenging ability than Control 1 treated with ascorbic acid, and also strong free radicals compared to Control 2 treated with methyl alcohol. It was confirmed that it exhibits an elimination effect.
실시예Example 3-2: 세포증식 3-2: cell proliferation 활성능active ability 측정 measurement
피부세포 증식 활성화에 대하여 왕불유행 추출물이 미치는 영향을 확인하기 위해, 사람의 섬유아세포를 사용하여 세포증식 활성능을 측정하였다. In order to confirm the effect of the extract of Wangbulbyeongyeon on the activation of skin cell proliferation, the cell proliferation activity was measured using human fibroblasts.
다양한 농도(0.01 ㎍/㎖, 0.1 ㎍/㎖, 1 ㎍/㎖, 10 ㎍/㎖)의 왕불유행 추출물을 배양액에 첨가한 후, 사람의 섬유아세포를 배양하여 MTT 환원법을 수행하고, 포르마잔(formazan) 생성물을 이소프로필 알코올로 추출하여, 570 nm에서 흡광도를 측정함으로써 세포증식 활성능을 상대비교하였다. 이때, 대조군으로는, FBS(fetal bovine serum) 및 왕불유행 추출물을 넣지 않은 배양액를 사용한 음성대조군, 10% FBS를 넣은 배양액를 사용한 양성대조군, 두 가지를 사용하였다. After adding to the culture medium the extract of Wangbulbongbyeong at various concentrations (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml), the MTT reduction method was performed by culturing human fibroblasts, and formazan ( formazan) product was extracted with isopropyl alcohol, and the cell proliferation activity was compared by measuring the absorbance at 570 nm. At this time, as a control group, two types were used: a negative control group using a culture medium containing no fetal bovine serum (FBS) and extracts of Wangbulbyeongheung, and a positive control group using a culture medium containing 10% FBS.
5회 반복 시험하여 측정한 결과를 하기 수학식 1을 이용하여 수치로 계산하였고, 하기 표 3에 정리하였다. The results of the repeated test 5 times were calculated numerically using Equation 1 below, and are summarized in Table 3 below.
[수학식 1][Equation 1]
세포증식능cell proliferation (%) = 각 (%) = each 시료군에서의in the sample group 흡광도/대조군에서의 흡광도 Absorbance/Absorbance in Control
왕불유행 추출물
King Bullfinch Extract
상기 표 3의 결과에서 보듯이, 왕불유행 추출물을 처리한 경우, 최소 실험 처리 농도(0.01 ㎍/㎖)에서 113.1%의 세포증식 활성능을 보였으며, 이는 아무것도 처리하지 않은 대조군의 세포증식 활성능(100%)에 비하여 증가한 것임을 확인하였다. As shown in the results of Table 3 above, when the extract of Wangbulbyeongheung was treated, 113.1% of cell proliferation activity was exhibited at the minimum experimental treatment concentration (0.01 μg/ml), which was the cell proliferation activity of the untreated control group. (100%) was confirmed to be increased.
따라서, 본 발명의 왕불유행 추출물은 세포증식을 통한 세포활성 효과가 탁월하여 손상된 피부의 재생을 위한 화장료 조성물, 예컨대 피부 외용제뿐만 아니라 약학적 조성물 또는 건강기능식품 조성물로도 유용하게 사용될 수 있음을 확인하였다.
Therefore, the extract of the present invention confirms that it can be usefully used as a cosmetic composition for the regeneration of damaged skin, such as a pharmaceutical composition or a health functional food composition as well as a pharmaceutical composition or health functional food composition, as well as a cosmetic composition for the regeneration of damaged skin due to its excellent cell activity effect through cell proliferation did
제조예production example 1 및 1 and 비교예comparative example 1: 피부 외용제의 제조 1: Preparation of external preparation for skin
하기 표 4의 조성과 같이, 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 피부 외용제를 통상의 방법에 따라 제조하였다(제조예 1). 또한, 유효성분을 제외한 나머지 성분으로 제조한 피부 외용제를 비교예 1의 조성으로 제조하였다.As shown in the composition shown in Table 4 below, an external preparation for skin whitening or regeneration containing the extract of Wangbulfangi as an active ingredient was prepared according to a conventional method (Preparation Example 1). In addition, a skin external preparation prepared with the remaining ingredients except for the active ingredient was prepared according to the composition of Comparative Example 1.
제조예production example 2 및 2 and 비교예comparative example 2: 에센스의 제조 2: Preparation of essence
하기 표 5의 조성과 같이, 왕불유행 추출물을 유효성분으로 포함하는 피부 미백 또는 재생용 에센스를 통상의 방법에 따라 제조하였다(제조예 2). 또한, 유효성분을 제외한 나머지 성분으로 제조한 에센스를 비교예 2의 조성으로 제조하였다.As shown in the composition of Table 5 below, a skin whitening or regenerating essence containing the extract of Wangbulin dynamite as an active ingredient was prepared according to a conventional method (Preparation Example 2). In addition, an essence prepared with the remaining ingredients excluding the active ingredient was prepared according to the composition of Comparative Example 2.
실시예Example 5: 관능실험 5: Sensory experiment
실시예Example 5-1: 색소침착 저해 효과 분석 5-1: Analysis of pigmentation inhibition effect
피부 색소침착 저해에 대한 상기 제조예 1 및 2의 조성에 따라 제조한 피부 외용제 및 에센스의 효과를 확인하기 위하여, 비교 관능실험을 수행하였다. In order to confirm the effect of the skin external preparations and essences prepared according to the compositions of Preparation Examples 1 and 2 on the inhibition of skin pigmentation, a comparative sensory experiment was performed.
건강한 남·여 20명을 선정하여 양팔의 하박부에 직경 7 ㎜ 구멍이 6개씩 두 줄로 파인 알루미늄 호일을 붙이고, 팔에서 10 ㎝ 떨어진 거리에서 ORIEL solar simulator 1000W를 사용하여 60 mJ/㎠의 광량을 조사하였다. 조사 전에 70% 에탄올 수용액으로 조사 부위를 세척하고 실험을 진행하였다. 조사 3일 전부터 조사 후 3주째까지 1일 2회씩 상기 제조예 1 및 2, 또한 비교예 1 및 2에 따라 제조한 피부 외용제 및 에센스를 각 한 줄에 도포하였다. 색소침착도를 육안으로 판정하고, 상기 비교예에 대하여 상기 제조예가 색소침착을 억제한 정도를 효과 있음, 또는 효과 없음의 2 단계로 평가하였으며, 결과를 하기 표 6에 나타내었다. 20 healthy men and women were selected, and aluminum foil with six 7 mm diameter holes was attached to the lower arm of both arms in two rows, and 60 mJ/cm2 of light was applied at a distance of 10 cm from the arm using ORIEL solar simulator 1000W. investigated. Before irradiation, the irradiation site was washed with 70% ethanol aqueous solution and the experiment was carried out. The external skin preparations and essences prepared according to Preparation Examples 1 and 2 and Comparative Examples 1 and 2 were applied twice a day from 3 days before the irradiation until the 3rd week after the irradiation in one line. The degree of pigmentation was visually determined, and the degree of suppression of pigmentation of the Preparation Example with respect to the Comparative Example was evaluated in two stages: effective or ineffective, and the results are shown in Table 6 below.
상기 표 6의 결과에서 보듯이, 상기 본 발명의 왕불유행 추출물을 함유하는 피부 외용제 및 에센스가 왕불유행 추출물을 함유하지 않은 비교예의 피부외용제 및 에센스에 비하여 미백 효과를 나타내었으며, 피부 내에서 어떠한 부작용도 나타내지 않는다는 것을 확인하였다. As shown in the results of Table 6, the skin external preparation and essence containing the extract of the present invention showed a whitening effect compared to the external preparation and the essence of the comparative example that did not contain the extract of Wangbulinaceae of the present invention, and there were no side effects in the skin. It was also confirmed that not shown.
따라서, 본 발명의 왕불유행 추출물 또는 이를 포함하는 조성물은 기미나 주근깨의 개선 또는 피부 미백을 위한 피부 외용제, 또는 화장료로 유용하게 사용할 수 있음을 확인하였고, 피부에 대하여 안전하다는 것을 추가로 확인하였다.
Therefore, it was confirmed that the extract or a composition comprising the extract of the present invention can be usefully used as an external preparation for skin or cosmetics for improving spots or freckles or for skin whitening, and it was further confirmed that it is safe for the skin.
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로서 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.
Claims (10)
상기 왕불유행은 장구채(Melandrium firmum) 및 애기장구채(Melandrium apricum)의 종자인 것인, 화장료 조성물.
A cosmetic composition for skin whitening, regenerating or antioxidant containing extract ( Melandrium firmum ) as an active ingredient,
The Wangbul epidemic is Jangguchae ( Melandrium firmum ) and Arabidopsis Jangguchae ( Melandrium apricum ) The seed of the, cosmetic composition.
According to claim 1, wherein the composition is a cosmetic composition comprising 0.01 wt% to 10 wt% of the extract of Wangbulbyeonghyeong relative to the total weight of the composition.
According to claim 1, wherein the skin whitening cosmetic composition, characterized in that due to melanin synthesis inhibitory activity.
The cosmetic composition according to claim 1, wherein the skin antioxidant is due to free radical scavenging ability.
The cosmetic composition according to claim 1, wherein the skin regeneration is due to cell proliferation promoting activity.
According to claim 1, wherein the cosmetic composition is a solution, external ointment, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil , suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray. cosmetic composition.
상기 왕불유행은 장구채(Melandrium firmum) 및 애기장구채(Melandrium apricum)의 종자인 것인, 건강기능식품 조성물.
It is a health functional food composition for skin whitening, regeneration or antioxidant containing extract ( Melandrium firmum ) as an active ingredient,
The Wangbul epidemic is Jangguchae ( Melandrium firmum ) and Arabidopsis Jangguchae ( Melandrium apricum ) The seeds of the, health functional food composition.
According to claim 7, wherein the health functional food composition is meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcoholic beverage and vitamin A health functional food composition, characterized in that it has a formulation selected from the group consisting of complex agents.
상기 왕불유행은 장구채(Melandrium firmum) 및 애기장구채(Melandrium apricum)의 종자인 것인, 의약외품 조성물.
It is a quasi-drug composition for skin whitening, regeneration, or antioxidation containing the extract ( Melandrium firmum ) as an active ingredient,
The Wangbul epidemic is Jangguchae ( Melandrium firmum ) and Arabidopsis Jangguchae ( Melandrium apricum ) The seeds of the, quasi-drug composition.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024090952A1 (en) * | 2022-10-27 | 2024-05-02 | 경희대학교 산학협력단 | Composition for preventing or treating radiation-resistant head and neck cancer, comprising melandrium herb extract as active ingredient |
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| KR102656259B1 (en) * | 2016-12-31 | 2024-04-09 | 조이앤코퍼레이션(주) | Cosmetic composition generating carbon dioxide with excellent shelf life in in hign temperature environment |
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| KR101589793B1 (en) * | 2013-08-27 | 2016-01-28 | 영남대학교 산학협력단 | Composition for inhibiting cellular senescence comprising extracts of Melandrium firmum Rohrbach or bornesitol isolated from the same |
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| 경락경혈학회지, 제26권, 제4호, 107∼118쪽, 2009년12월 공개* |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024090952A1 (en) * | 2022-10-27 | 2024-05-02 | 경희대학교 산학협력단 | Composition for preventing or treating radiation-resistant head and neck cancer, comprising melandrium herb extract as active ingredient |
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