KR102176883B1 - Composition for skin regeneration, anti-wrinkle, or wound healing containing Prunus persica resin - Google Patents

Abstract

The present invention provides a cosmetic composition, a pharmaceutical composition, and a health functional food composition containing a Prunus persica resin or an extract thereof as an active component. The composition contains the Prunus persica resin or the extract thereof, thereby having excellent effects of skin regeneration, wrinkle alleviation, wound alleviation, or wound healing. Therefore, the composition can be used as the cosmetic composition, the pharmaceutical composition, the health functional food composition, etc. for skin regeneration, wrinkle alleviation, wound alleviation, or wound healing.

Description

Composition for skin regeneration, anti-wrinkle, or wound healing containing Prunus persica resin}

It relates to a composition for skin regeneration, wrinkle improvement, or wound healing.

Radiant tree extract has a slightly yellowish consistency, and turns into a hard form when dried. It is also called Taoism in China and other Asian countries, and is rich in protein and collagen, so it is consumed for food and used as a medicine.

Non-Patent Document 1 discloses that the oligosaccharide derived from pitch gum has antioxidant activity and antibacterial activity.

However, there are no studies on the effects of radix tree resin on skin regeneration and wound healing.

Xing-Cun Yao et al., “Antioxidant Activity and Antibacterial Activity of Peach Gum Derived Oligosaccharides”, International journal of biological macromolecules, Vol. 62 (2013)

It provides a cosmetic composition for skin regeneration, wrinkle improvement, or wound improvement, containing a radish tree resin or an extract thereof as an active ingredient.

It provides a pharmaceutical composition for skin regeneration, wrinkle improvement, or wound healing containing a radish tree resin, or an extract thereof, as an active ingredient.

It provides a health functional food composition for skin regeneration, wrinkle improvement, or wound improvement, containing a copy tree resin, or an extract thereof as an active ingredient.

It provides a method of skin regeneration, wrinkle improvement, or wound improvement comprising the step of applying or administering to a subject an effective amount of the above composition.

One aspect provides a cosmetic composition for skin regeneration, wrinkle improvement, or wound improvement, comprising a radix tree resin or an extract thereof as an active ingredient.

In one embodiment, it was confirmed that the radiant tree resin has an excellent effect of regenerating the skin, improving wrinkles and healing wounds accordingly.

“Peach resin” refers to a resin obtained from Prunus Persica , and can be used interchangeably with the terms “peach resin”, “copy tree sword”, “pitch gum” and “Taoism”. The radiant tree resin may be a radiant tree extract itself, or a substance obtained from the radiant tree extract, and may be, for example, a gum based on a polysaccharide obtained by separating and extracting the radiant tree extract.

In the present specification, the copy tree resin may be a copy tree resin itself, or an extract of the copy tree resin. The "extract" may include an extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product or purified product thereof, and a fraction obtained by fractionating the product. In one embodiment, the radiant tree resin is Prunus Persica (Peach) Resin Extract. The radiant tree resin extract may be an extract extracted from the radiant tree resin using a conventional solvent. For example, the extraction solvent may be an alcohol such as ethanol. In another embodiment, the radiant tree resin is a concentrate of the radiant tree resin extract.

“Containing as an active ingredient” means that radix tree resin, its extract, or a concentrate of its extract is added to the extent that it can exhibit the above-mentioned effects, and various ingredients are added as auxiliary ingredients for drug delivery and stabilization. It is meant to include being formulated in various forms.

"Skin regeneration" may mean any action that supplements a part of the skin when it is lost or damaged or promotes the proliferation and migration of skin cells. As the skin regenerates, wrinkles may improve.

"Wrinkle" refers to a state in which the elasticity of the skin is lost and loosened, for example, the skin may be folded.

“Improvement” may refer to any action that at least reduces the severity of a parameter related to the alleviation or treatment of the condition, eg symptom.

“Wrinkle improvement” can mean any action that thins or reduces wrinkles on the face or body.

“Wound” is also referred to as “wound” and means damage to a living body. The damage may be due to external factors such as physical damage, radiation, stimulation by chemical substances, proliferation of microorganisms, and internal factors such as stress, but is not limited thereto. The wound may include all kinds of wounds, such as a cut window, a javelin, a halved window, an acne, a fissure, a slit, and a school window.

“Wound improvement” can mean any action that lightens or reduces the severity of a face or body wound.

“Wound healing” can refer to a series of biological reactions to repair the wound.

The cause of skin regeneration, wrinkle improvement, wound improvement, or wound healing is not limited, but may be due to, for example, migration or proliferation of skin cells.

The radiation tree resin, or an extract thereof, is 1 to 100,000 ppm, 1 to 50,000 ppm, 1 to 10,000 ppm, 1 to 5,000 ppm, 1 to 3,000 ppm, 1 to 2,000 ppm, 1 to 1,000 ppm, 1 to 500 ppm for the composition. ppm, 1 to 100 ppm, 10 to 10,000 ppm, 10 to 5,000 ppm, 10 to 3,000 ppm, 10 to 2,000 ppm, 10 to 1,000 ppm, 10 to 500 ppm, or 10 to 100 ppm. If the concentration of the radiant tree resin is too low, the intended skin regeneration, wrinkle improvement, and wound improvement effects may not appear. If the concentration of radiant tree resin exceeds 100,000 ppm, discomfort due to odor may occur.

The cosmetic composition may further contain any one or more of Centella asiatica extract, Madecassoside, and Staphylococcus epidermidis strain culture solution.

“ Centella Asiatica Extract” is also called “Centella Extract”, and is an extract extracted from the outpost of Centella Asiatica .

In one embodiment, it was confirmed that a synergistic wound healing effect appeared when using a combination of radix tree resin and centella asiatica extract. Specifically, it was confirmed that when 100 ppm of radix tree resin and 10 ppm of centella asiatica were treated together, the synergistic wound recovery effect was excellent compared to the case where each was treated alone.

The centella extract is 1 to 100,000 ppm, 1 to 50,000 ppm, 1 to 10,000 ppm, 1 to 5,000 ppm, 1 to 3,000 ppm, 1 to 2,000 ppm, 1 to 1,000 ppm, 1 to 500 ppm, or 1 to It may be contained in a concentration of 100 ppm.

The composition may contain the radix tree resin and centella asiatica extract in a weight ratio of 1:1 to 100:1, for example, in a weight ratio of 10:1, but is not limited thereto.

“Madecassoside” is a compound extracted from Centella Asiatica .

The Staphylococcus epidermidis strain may be a known strain. For example, it may be a Staphylococcus epidermidis ST-6 strain (accession number: KCCM12551P), but is not limited thereto. The Staphylococcus epidermidis strain culture solution may be a culture solution obtained by culturing the Staphylococcus epidermidis strain in TSB (Tryptic soy broth) medium, but is not limited thereto.

In one embodiment, it was confirmed that a synergistic wound healing effect was exhibited when using a combination of a radiation tree resin, centella asiatica extract, madecassoside, and Staphylococcus epidermidis strain culture solution. Specifically, when treated with 100 ppm of radish tree resin, 10 ppm of centella asiatica extract, 10 ppm of madecassoside, and 1% (v/v) of the strain culture medium of Staphylococcus epidermidis Compared to one case, it was confirmed that the synergistic wound healing effect was superior.

The madecassoside is 1 to 100,000 ppm, 1 to 50,000 ppm, 1 to 10,000 ppm, 1 to 5,000 ppm, 1 to 3,000 ppm, 1 to 2,000 ppm, 1 to 1,000 ppm, 1 to 500 ppm, or 1 It may be contained in a concentration of to 100 ppm.

The Staphylococcus epidermidis strain culture solution is 0.01 to 5% (v/v), 0.01 to 3% (v/v), 0.01 to 1% (v/v), 0.1 to It may be contained in a concentration of 5% (v/v), 0.1 to 3% (v/v), or 0.1 to 1% (v/v).

The cosmetic composition is a lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, It may be prepared in the form of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, suspension, gel, powder, paste, mask pack, mask sheet, or aerosol composition, but is not limited thereto. Compositions of these formulations can be prepared according to methods conventional in the art.

The cosmetic composition may further include a cosmetically acceptable excipient or carrier. The types of cosmetically acceptable excipients or carriers vary according to the formulation and purpose, and as usual ingredients, for example, water, surfactant, alcohol, oil, thickener, chelating agent, colorant, preservative, fragrance, etc. It can, but is not limited thereto. The blending amount of the excipient or carrier can be easily determined by a person skilled in the art within a range that does not impair the object and effect of the present invention.

Another aspect provides a pharmaceutical composition for skin regeneration, wrinkle improvement, or wound healing comprising a radix tree resin, or an extract thereof, as an active ingredient.

Here, the explanations for “Radiation Tree Resin”, “Containing as an active ingredient”, “Skin regeneration”, “Wrinkle improvement”, and “Wound healing” are as described above.

The radiation tree resin, or an extract thereof, is 1 to 100,000 ppm, 1 to 50,000 ppm, 1 to 10,000 ppm, 1 to 5,000 ppm, 1 to 3,000 ppm, 1 to 2,000 ppm, 1 to 1,000 ppm, 1 to 500 ppm for the composition. ppm, 1 to 100 ppm, 10 to 10,000 ppm, 10 to 5,000 ppm, 10 to 3,000 ppm, 10 to 2,000 ppm, 10 to 1,000 ppm, 10 to 500 ppm, or 10 to 100 ppm. When the concentration of the radix tree resin or its extract is too low, the desired effect of skin regeneration, wrinkle improvement, and wound improvement may not appear. When the concentration of the radix tree resin or its extract exceeds 100,000 ppm, discomfort due to odor may occur.

The pharmaceutical composition may further contain any one or more of Centella asiatica extract, Madecassoside, and Staphylococcus epidermidis strain culture solution.

Here, the description of “central grass extract”, “madecassoside”, “ Staphylococcus epidermidis strain culture solution” and its contents are as described above.

The pharmaceutical composition may be formulated as an oral or parenteral dosage form. The oral dosage form may be a granule, powder, liquid, tablet, capsule, dry syrup, or a combination thereof. The parenteral dosage form may be an injection or an external preparation for the skin. The pharmaceutical composition may further include a pharmaceutically acceptable diluent or carrier. The kind of the pharmaceutically acceptable diluent or carrier varies depending on the formulation and purpose. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and as a lubricant, magnesium stearate, talc, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, anhydrous calcium monohydrogen phosphate, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof. The blending amount of the diluent or carrier can be easily determined by a person skilled in the art within a range that does not impair the object and effect of the present invention.

In one embodiment, the pharmaceutical composition may be an external preparation for skin. The external preparation for skin may be prepared in a formulation such as a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, etc., but is not limited thereto. The above skin external preparations are ingredients commonly used in external preparations for skin such as cosmetics or pharmaceuticals, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances. , Colorants, various skin nutrients, or combinations thereof and may be appropriately formulated according to need. The external preparations for skin include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glavidine, and caline. Hot water extract of fruit, various herbal medicines, drugs such as tocopherol acetate, glytilithic acid, tranexamic acid and derivatives or salts thereof, vitamin C, magnesium ascorbate, glucoside ascorbic acid, arbutin, kojic acid, glucose, fructose, Sugars such as trehalose can also be appropriately blended. The amount of the ingredients used in the external preparation for skin can be easily determined by a person skilled in the art within a range that does not impair the object and effect of the present invention.

Another aspect provides a health functional food composition for skin regeneration, wrinkle improvement, or wound improvement, comprising a radix tree resin, or an extract thereof, as an active ingredient.

Here, the explanations for “copy tree resin”, “contained as an active ingredient”, “skin regeneration”, “wrinkle improvement” and “wound improvement” are as described above.

The health functional food composition may further contain any one or more of centella extract, Madecassoside, and Staphylococcus epidermidis strain culture solution.

Here, the description of “central grass extract”, “madecassoside”, “ Staphylococcus epidermidis strain culture solution” and its contents are as described above.

The health functional food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the kind of the health functional food. Among the types of health functional foods, the beverage composition may contain various flavoring agents or natural carbohydrates as an additional component, like a conventional beverage. The natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame can be used. The health food composition may also be used in nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonated beverages. Carbonating agents used, or combinations thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverage, pulp for the production of vegetable beverages, or a combination thereof.

Another aspect provides a method for skin regeneration, wrinkle improvement, or wound improvement comprising the step of applying or administering to a subject an effective amount of the above composition.

“Individual” means any animal, including humans, in need of skin regeneration, wrinkle improvement, or wound improvement.

“Apply” means any act of contacting or transferring the substance to an object in an appropriate way so that the effect of the substance can be exerted on the object. The application may be a method of applying to the skin.

“Administering” means placing a substance into a subject or a method that results in at least partial localization of the subject to a desired site. Administration may be administered by a method known in the art. Administration can be administered directly to a subject by any means, for example by routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. I can. The administration can be administered systemically or locally.

The application or dosage may be variously prescribed depending on factors such as formulation method, mode of administration, age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the individual, and those skilled in the art If so, the application or dosage can be appropriately adjusted in consideration of these factors. The number of times of application or administration may be once a day or two or more times within the range of clinically acceptable side effects, and may be applied or administered at one or two or more sites for the application or administration site, and daily or 2 The total number of days of application or administration at intervals of 5 to 5 days may be applied or administered from 1 to 30 days in a single treatment. If necessary, the same treatment can be repeated after an appropriate time period. For non-human animals, the same dose per kg as human or, for example, the volume ratio (e.g., average value) of the target animal and the human organ (heart, etc.) One amount can be administered.

All technical terms used above, unless otherwise defined, are used in the same meaning as those of ordinary skill in the art generally understand.

The composition according to an aspect is excellent in skin regeneration, wrinkle improvement, wound improvement, or wound healing effect by containing the radix tree resin, or an extract thereof. Therefore, it can be used as a cosmetic composition, pharmaceutical composition, health functional food composition, etc. for skin regeneration, wrinkle improvement, wound improvement, or wound healing.

1 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 1.
2 is a graph showing wound recovery results after 24 hours of treatment according to Experimental Example 1.
3 is a micrograph showing the wound recovery results after 0 hours and 24 hours treatment according to Experimental Example 1.
4 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 2.
5 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 2.
6 is a graph showing wound recovery results after 16 hours of treatment according to Experimental Example 2.
7 is a micrograph showing the wound recovery results after 0 hours and 16 hours treatment according to Experimental Example 2.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1. Preparation of radiant tree resin

Add 50g of copy tree resin per 200ml of 70% ethanol and extract for 72 hours to obtain an extract. The extract was concentrated to 1 ml using a concentrator to obtain a concentrate. The concentrate of the radix tree resin extract was used in the following experiment.

Experimental Examples 1 and 2. Evaluation of skin regeneration and wound healing efficacy

In order to evaluate the skin regeneration and wound healing efficacy of the rhizome resin, a cell migration assay was performed.

Preparation of test substances

For Experimental Example 1 and Experimental Example 2, test substances were prepared as shown in Table 1 below. In Table 1 below, “ST-6” refers to a culture solution obtained by culturing Staphylococcus epidermidis ST-6 strain (accession number: KCCM12551P) in TSB (Tryptic soy broth) medium (BD Difco™). it means.

Experimental example
(Processing time) designation Treatment material and concentration Experimental Example 1
(24 hours) Negative control (-) Untreated P 10 Radiant Tree Resin 10 ppm P 100 Radiant Tree Resin 100 ppm C 10 Byeongpung extract 10 ppm P 10 + C 10 Radiant tree resin 10 ppm + folding screen extract 10 ppm P 100 + C 10 Radiant tree resin 100 ppm + folding screen extract 10 ppm Experimental Example 2
(16 hours) Negative control (-) Untreated P 10 Radiant Tree Resin 10 ppm P 100 Radiant Tree Resin 100 ppm C 10 Byeongpung extract 10 ppm M 10 Madecassoside 10 ppm ST-6 1 Staphylococcus epidermidis ST-6 strain culture medium 1% (v/v) P 10 mix Radix tree resin 10 ppm + folding screen extract 10 ppm + madecassoside 10 ppm + ST-6 strain culture solution 1% (v/v) P 100 mix Radix tree resin 100 ppm + screen extract 10 ppm + madecassoside 10 ppm + ST-6 strain culture solution 1% (v/v)

Experimental procedure

On the first day, Hs68 human fibroblasts were aliquoted into 96-well ImageLock™ plates (No. 4379; Essen BioScience) at 2×10 ⁴ cells/well. The experiment was conducted at 90% cell confluent.

On the second day, WoundMaker™ (Essen BioScience, USA) was washed with 45 ml of 70% ethanol solution for 5 minutes and then with 45 ml of distilled water for 5 minutes. Washed WoundMaker™ (Essen BioScience, USA) was used to scratch all cell layers of each well of the plate. The medium was removed and washed with PBS. After filling the serum-free medium, each test substance in Table 1 was treated.

On the third day, using the IncuCyte ZOOM (Essen BioScience, USA) program, the degree of healing of the wound was measured and graphed at 2 hour intervals. In addition, the degree of wound healing was visually confirmed by checking the cell picture measured by the microscope inside the device.

Experiment result

1 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 1.

2 is a graph showing wound recovery results after 24 hours of treatment according to Experimental Example 1.

3 is a micrograph showing the wound recovery results after 0 hours and 24 hours treatment according to Experimental Example 1.

As can be seen in FIGS. 1 to 3, when the radiation tree resin is treated, a wound healing effect was exhibited by cell migration. In addition, it was confirmed that a synergistic wound recovery effect appeared when the radix tree resin was treated with centella asiatica extract. In particular, when treated with 100 ppm of radix tree resin and 10 ppm of centella asiatica extract, it was confirmed that the synergistic wound recovery effect was excellent compared to the case where each was treated alone.

4 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 2.

5 is a graph showing wound recovery results measured at 2 hour intervals according to Experimental Example 2.

6 is a graph showing the wound recovery results after 16 hours of treatment according to Experimental Example 2.

7 is a micrograph showing the wound recovery results after 0 hours and 16 hours treatment according to Experimental Example 2.

As can be seen in FIGS. 4 to 7, when the radiation tree resin is treated, the wound healing effect was exhibited by cell migration. In addition, it was confirmed that a synergistic wound recovery effect appeared when the radix tree resin was treated with centella asiatica extract and madecassoside. In particular, 100 ppm of radix tree resin, 10 ppm of centella asiatica extract, 10 ppm of madecassoside, and 1% (v/v) of the strain culture solution of Staphylococcus epidermidis were treated together. Compared to the case, it was confirmed that the synergistic wound healing effect was excellent.

Therefore, it was found that the radiant tree resin has skin regeneration, wrinkle improvement, wound improvement or healing effect.

Korea Microorganism Conservation Center (overseas) KCCM12551P 20190611

Claims (7)

A cosmetic composition for improving wounds containing a radish tree resin or an extract thereof as an active ingredient. The cosmetic composition of claim 1, wherein the copy tree resin, or an extract thereof, is contained in a concentration of 1 to 100,000 ppm based on the composition. The cosmetic composition of claim 1, wherein the cosmetic composition further contains any one or more of centella extract, Madecassoside, and Staphylococcus epidermidis strain culture solution. A pharmaceutical composition for healing wounds containing a radiation tree resin, or an extract thereof as an active ingredient. The pharmaceutical composition for wound healing according to claim 4, wherein the radiation tree resin, or an extract thereof, is contained in a concentration of 1 to 100,000 ppm based on the composition. The pharmaceutical composition for wound healing according to claim 4, wherein the pharmaceutical composition further comprises any one or more of centella extract, Madecassoside, and Staphylococcus epidermidis strain culture. A health functional food composition for improving wounds containing a radiation tree resin, or an extract thereof as an active ingredient.

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