KR102204368B1 - Composition for promoting synthesis of hyaluronic acid comprising jujube extracts and the use thereof - Google Patents

Abstract

Since the jujube extract or its fraction effectively promotes the production of hyaluronic acid and the expression of hyaluronic acid synthase, it can be usefully used for joint protection, arthritis prevention or treatment, skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment, and skin moisturization. have.

Description

Composition for promoting synthesis of hyaluronic acid comprising jujube extracts and the use thereof {Composition for promoting synthesis of hyaluronic acid comprising jujube extracts and the use thereof}

The present invention relates to a composition for promoting hyaluronic acid production comprising a jujube extract or a fraction thereof as an active ingredient.

In addition, the present invention relates to a pharmaceutical composition for joint protection or arthritis prevention or treatment, a quasi-drug composition for joint protection or arthritis prevention or improvement, or a food composition comprising the composition as an active ingredient.

In addition, the present invention is a cosmetic composition, or a food composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, comprising the composition for promoting production of hyaluronic acid as an active ingredient; Or it relates to a pharmaceutical composition for preventing or treating skin wrinkles.

In addition, the present invention relates to a skin moisturizing cosmetic composition, food composition, or quasi-drug composition comprising the composition for promoting production of hyaluronic acid as an active ingredient.

Hyaluronic acid (hyaluronic acid) is a type of glycosaminoglycans (glycosaminoglycans) is a chain-shaped polymer polysaccharide substance in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked. It combines with a large amount of water to form a gel, so it has high viscosity and elasticity. Hyaluronic acid, as a major component of the extracellular matrix, is reported to be involved in water retention, maintenance of intercellular gaps, storage and diffusion of cell growth factors and nutrients, as well as cell division, differentiation, and migration. have.

In joints, hyaluronic acid is a major component of synovial fluid that protects joints, and is secreted from fibrolast-like synoviocytes. Hyaluronic acid works to lubricate the joints, and a decrease in hyaluronic acid is one of the causes of osteoarthritis, which reduces the shock absorption of the joint and makes the joint stiff, making walking uncomfortable. As the hyaluronic acid in the joint decreases, the joint cannot absorb or disperse external shocks, resulting in severe joint damage.

The concentration of hyaluronic acid in the synovial fluid is determined by the production of hyaluronic acid by hyaluronic acid synthase (HAS) and the degradation of hyaluronic acid by hyaluronic acid decomposition enzyme (Hyaluronidase, HYAL). In patients with degenerative arthritis and rheumatoid arthritis, the expression of HAS-1 and HAS-2 in the synovial membrane is decreased, and the expression of HYAL-2 is increased (Arthritis Res Ther, 2004, 6, R514-R520).

On the other hand, in the skin, it has been reported that more than 50% of hyaluronic acid in the mammalian body is distributed in the skin, especially in the intercellular spaces of the epidermis and the connective tissues of the dermis, and this hyaluronic acid is mainly synthesized by keratinocytes and fibroblasts. Is known to be. It has been reported that the amount of hyaluronic acid in human skin decreases with aging, and the decrease in hyaluronic acid in the skin is considered to be one of the direct causes of the decrease in skin elasticity and moisture content according to aging (Biochem Biophys Acta 279, 265-275 ; Carbohydr Res 159, 127-136; Int J Dermatol 33, 119-122). It is also known that hyaluronic acid is involved in maintaining the structure of the stratum corneum and maintaining the skin barrier function (J Cosmet Dermatol. 2007 Jun, 6(2), 75-82).

However, hyaluronic acid having the above effects is difficult to ingest as food and is not well absorbed. In addition, a method of alleviating arthritis by injecting hyaluronic acid into a joint has been approved by the US FDA in 1997 and is currently being performed, but ultimately, a method of increasing the synthesis of hyaluronic acid in the body is more effective in order to solve arthritis. Therefore, research on a method capable of increasing the production of hyaluronic acid in the human body is being actively conducted, but remarkable research results are not yet known.

Under this background, the present inventors have endeavored to develop a substance capable of increasing the synthesis of hyaluronic acid in the body, and as a result, a composition comprising a jujube extract or a fraction thereof promotes the production of hyaluronic acid and promotes the production of hyaluronic acid synthase (HAS). As it increases expression, it protects joints, prevents or treats arthritis, improves skin elasticity, prevents aging, prevents or treats skin wrinkles; And it was confirmed that there is a skin moisturizing effect and completed the present invention.

One object of the present invention is to provide a composition for promoting hyaluronic acid production comprising a jujube extract or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a composition for joint protection or arthritis prevention or treatment comprising the composition as an active ingredient.

Another object of the present invention is to provide a composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, comprising the composition for promoting production of hyaluronic acid as an active ingredient.

Another object of the present invention is to provide a composition for moisturizing skin comprising the composition for promoting production of hyaluronic acid as an active ingredient.

Another object of the present invention is to provide a composition wherein the composition is used as any one selected from the group consisting of cosmetic compositions, food compositions, pharmaceutical compositions, and quasi-drug compositions.

Another object of the present invention is to provide a composition wherein the composition comprises a jujube extract or a fraction thereof in an amount of 0.00001 to 10% by weight based on the total weight of the composition.

One object of the present invention is to provide a composition for promoting hyaluronic acid production comprising a jujube extract or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for joint protection or arthritis prevention or treatment, a quasi-drug composition for joint protection or arthritis prevention or improvement, or a food composition comprising the composition as an active ingredient.

Another object of the present invention is a cosmetic composition, or a food composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, comprising the composition for promoting production of hyaluronic acid as an active ingredient; Or to provide a pharmaceutical composition for preventing or treating skin wrinkles.

Another object of the present invention is to provide a skin moisturizing cosmetic composition, food composition, or quasi-drug composition comprising the composition for promoting hyaluronic acid production as an active ingredient.

In order to achieve the above object, one aspect of the present invention provides a composition for promoting hyaluronic acid production comprising a jujube extract or a fraction thereof as an active ingredient.

The term "jujube" of the present invention refers to a jujube tree belonging to the Rhamnaceae family ( Zizyphus jujuba ) means the fruit, and is known to have the effect of smoothing blood circulation and stabilizing nerves. The jujube may be purchased commercially, or may be grown or harvested in nature.

The term "extract" of the present invention refers to an extract obtained by the extraction treatment of the jujube, a diluted solution or a concentrate of the extract, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, the extract itself And extracts of all formulations that can be formed using the extract. Specifically, the extract of the present invention may be prepared and used in a dry powder form after extraction.

The extract may be extracted from natural, hybrid or variant plants of the plant, and may be extracted from plant tissue culture.

The method for preparing the jujube extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods. In addition, in order to obtain an extract of high purity, the extract may be extracted one or more times in the same manner.

The kind of extraction solvent used for the preparation of the jujube extract is not particularly limited, and any solvent known in the art may be used as long as the extract having the desired effect of the present invention can be obtained. For example, one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, or chloroform may be used. More specifically, ethanol can be used as a solvent.

The term "fraction" in the present invention means a result obtained by performing fractionation in order to separate a specific component or a specific group of components from a mixture containing several different constituents.

The fractionation method for obtaining the fraction is not particularly limited, and as long as the fraction having the desired effect of the present invention can be obtained, it may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include a method of obtaining a fraction from the extract by treating a jujube extract obtained by adding a solvent to the jujube with a predetermined fractionation solvent.

The kind of the fractionation solvent is not particularly limited, and any solvent known in the art may be used as long as a fraction having the desired effect of the present invention can be obtained. Non-limiting examples of the fractionation solvent include organic solvents such as water, alcohol, ethyl acetate, acetone or chloroform. These may be used alone or in combination of two or more. Specifically, the fractionation solvent may be at least one selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, and chloroform, but is not limited thereto.

The term "hyaluronic acid" of the present invention is a kind of glycosaminoglycans and refers to a chain-shaped polymer polysaccharide material in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked.

In one embodiment of the present invention, the jujube extract increases the production of hyaluronic acid in a concentration-dependent manner in human fibroblast-like synovial cells derived from patients with degenerative arthritis, and a higher hyaluronic acid synthesis efficiency in an amount 200 times less than that of glucosamine (positive control). It was confirmed that represents (Table 2).

In addition, in human fibroblast-like synovial cells derived from patients with degenerative arthritis, jujube extract increased the expression of hyaluronic acid synthase, especially HAS-2 and HAS-3, and was similar to HAS-2 in an amount 200 times less than that of glucosamine. It was confirmed that the expression levels of and HAS-3 are shown (Table 3).

In addition, in human-derived normal fibroblast-like synovial cells, it was confirmed that the jujube extract increased hyaluronic acid production and showed similar hyaluronic acid concentration even in an amount 100 times less than that of glucosamine (Table 4).

In addition, the jujube extract also increased hyaluronic acid production in the rat joint cavity (Table 5). Through this, jujube extract is not only synovial cells, but also in It was found that it exhibited excellent hyaluronic acid production promoting effect in the rat joint cavity in vivo .

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating joint protection or arthritis comprising the composition as an active ingredient. The pharmaceutical composition may increase the expression of hyaluronic acid synthase (HAS).

The term "joint protection" of the present invention means preventing joint damage due to various causes, and hyaluronic acid is a major component of synovial fluid that protects joints, so when hyaluronic acid is reduced, the joint absorbs external shocks. Inability to disperse or cause joint damage or osteoarthritis may occur.

The term "arthritis" of the present invention means that the joint is inflamed due to various causes, and when hyaluronic acid is reduced, arthritis may occur. Specifically, the arthritis is osteoarthritis, degenerative arthritis, rheumatoid arthritis, spondyloarthropathy, ankylosing spondylitis, psoriatic arthritis, gout, bacterial arthritis, childhood rheumatoid arthritis, lupus, scleroderma, multiple sclerosis, fibromyalgia, multiple myositis, dermatitis, Behcet's disease, Reiter's syndrome, Lyme arthritis, adhesive arthritis, frozen shoulder, tendon synovitis, elbow head pouchitis, dequovain tendon synovitis, recurrent rheumatism, rheumatoid multiple muscle pain or adult Still's disease, more specifically degenerative arthritis or rheumatoid arthritis However, it is not limited thereto.

In one embodiment of the present invention, the jujube extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthetase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity It was confirmed that the hyaluronic acid concentration can be increased in (Tables 2, 3, 4 and 5). Hyaluronic acid lubricates joints, and it is known that osteoarthritis can occur when hyaluronic acid decreases, and jujube extract or a fraction thereof having an excellent effect of promoting hyaluronic acid production can be effectively used for joint protection or arthritis prevention or treatment.

The pharmaceutical composition of the present invention may be included in various weight% if it can exhibit joint protection or arthritis prevention or treatment effect, but specifically, it may contain a jujube extract or a fraction thereof in an amount of 0.00001 to 10% by weight relative to the total weight of the pharmaceutical composition. I can.

The term "prevention" of the present invention means any action of inhibiting or delaying the onset of arthritis by administration of the composition.

The term "treatment" of the present invention refers to any action in which the symptoms of arthritis already onset are improved or benefited by the administration of the composition.

In addition, the pharmaceutical composition of the present invention may further include a suitable carrier, excipient or diluent according to a conventional method. Carriers, excipients and diluents that may be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils, but are not limited thereto.

The pharmaceutical composition according to the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations, suppositories, or sterile injectable solutions according to a conventional method. have. Specifically, when formulated, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations contain at least one excipient, such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. to the compound. It can be prepared by mixing. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, syrups, and other excipients, such as water and liquid paraffin, which are commonly used simple diluents, such as wetting agents, sweeteners, fragrances, and preservatives. I can. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As a base for suppositories, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like can be used.

The composition of the present invention is administered in a pharmaceutically effective amount. The pharmaceutically effective amount refers to an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and does not cause side effects, and the effective dose level is the patient's health condition, type of disease, severity, The activity of the drug, its sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of excretion, the duration of treatment, factors including drugs used in combination or concurrently, and other factors well known in the medical field. Specifically, it is generally 0.01mg to 5000mg per 1 kg of the body weight of the administered individual, and may be dividedly administered once a day or several times a day at predetermined time intervals according to the judgment of a doctor or pharmacist, but is not limited thereto.

In one embodiment of the present invention, a male Sprague-Dawley Rat (Samtaco, Korea) weighing about 200 g was diluted with physiological saline, and 100 or 500 mg per 1 kg of the rat's weight was orally administered once a day for 15 days. , It was confirmed that the jujube extract according to the present invention increases hyaluronic acid production in the joint cavity of normal rats (Example 5).

In addition, the pharmaceutical composition of the present invention may be used alone or for joint protection; Or it may be used in combination with other pharmaceutically active compounds exhibiting an arthritis prophylactic or therapeutic effect or in an appropriate collection.

In addition, the pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans by various routes. The administration means providing a predetermined substance to the patient by any suitable method, and the route of administration of the composition of the present invention is not limited as long as it can reach the target tissue. For example, intra-articular administration, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, intrapulmonary administration, or rectal administration, but are not limited thereto. In addition, the pharmaceutical composition of the present invention may be administered by any device capable of moving the active substance to target cells.

Another aspect of the present invention provides a method for preventing or treating arthritis comprising administering the composition for promoting production of hyaluronic acid to an individual.

The terms "hyaluronic acid", "arthritis", "prevention", "treatment" and "administration" of the present invention are as described above.

The dosage, administration frequency and route of administration for the administration are as described above.

The term "individual" of the present invention refers to monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, mice, rabbits, or guinea pigs, including humans who have or may develop arthritis. It means all animals including, and by administering the composition of the present invention to an individual, the disease can be effectively prevented or treated.

Another aspect of the present invention provides a quasi-drug composition for joint protection or arthritis prevention or improvement comprising the composition for promoting production of hyaluronic acid as an active ingredient.

In one embodiment of the present invention, the jujube extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthetase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity Since it was confirmed that the hyaluronic acid concentration can be increased (Tables 2, 3, 4 and 5), the composition comprising the jujube extract or fraction can be effectively used for joint protection or arthritis prevention or improvement.

The terms "hyaluronic acid", "arthritis" and "prevention" of the present invention are as described above, and "improvement" refers to all actions in which symptoms of arthritis are improved or beneficially changed by administration of the composition of the present invention.

The term "quasi-drug" of the present invention refers to fibers, rubber products, or the like, which are used for the purpose of treating, alleviating, treating or preventing diseases of humans or animals, weakly acting on the human body or not directly acting on the human body, or Non-machine and similar, as an article corresponding to one of the preparations used for sterilization, insecticide and similar purposes to prevent infection, and used for the purpose of diagnosing, treating, alleviating, treating or preventing human or animal diseases. Means items that are not instruments, machines, or devices among items, and items other than those that are not instruments, machines, or devices among items used for the purpose of pharmacologically affecting the structure and function of a person or animal. It may be a hygiene product, but is not limited thereto.

When the jujube extract or fraction is added to a quasi-drug composition for the purpose of joint protection or arthritis prevention or improvement, the jujube extract or fraction may be added as it is or used with other quasi-drug components, and may be appropriately used according to a conventional method. . The mixing amount of the active ingredient can be appropriately determined according to the intended use.

The external preparation for skin is not particularly limited thereto, but may be prepared and used in the form of an ointment, lotion, spray, patch, cream, powder, suspension, gel or gel. The personal hygiene products are not particularly limited thereto, but specifically, soap, cosmetics, wet tissues, tissue paper, shampoo, skin cream, face cream, toothpaste, lipstick, perfume, makeup, foundation, cheek touch, mascara, eye shadow, sunscreen It can be a lotion, hair care product, air freshener gel, or cleaning gel. In addition, another example of the quasi-drug composition of the present invention is a disinfectant cleaner, a shower foam, a wet tissue, a detergent soap, a hand wash, a mask or an ointment.

The quasi-drug composition of the present invention may contain various weight percent if it can exhibit joint protection or arthritis preventing or improving effect, but specifically, jujube extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the quasi-drug composition. .

Another aspect of the present invention provides a food composition for preventing or improving joint protection or arthritis comprising the composition for promoting production of hyaluronic acid as an active ingredient. Since the jujube has been used for a long time as a natural substance and has proven its stability, it can be prepared and ingested in the form of a food that can protect joints or prevent or improve arthritis while having common sense.

In one embodiment of the present invention, the jujube extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthetase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity Since it was confirmed that the hyaluronic acid concentration can be increased (Tables 2, 3, 4 and 5), the composition comprising the jujube extract or fraction can be effectively used for joint protection or arthritis prevention or improvement.

The terms "hyaluronic acid", "arthritis", "prevention" and "improvement" of the present invention are as described above.

The term "food" of the present invention is meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, There are vitamin complexes, functional foods and health foods, and all foods in the usual sense are included.

The functional food is the same term as food for special health use (FoSHU), and refers to foods with high medical and medical effects processed so that the bioregulatory function is effectively displayed in addition to nutritional supply. . Here, the term "functionality" means obtaining a useful effect for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The functional food of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art can be added to prepare it. In addition, the formulation of the functional food may be prepared without limitation as long as it is a formulation recognized as a functional food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material, and is excellent in portability. Functional foods of can be consumed as a supplement to enhance the effect of preventing or improving arthritis.

The health food refers to a food having an active health maintenance or promotion effect compared to a general food, and a health supplement food refers to a food for health supplement purposes. In some cases, the terms functional food, health food, and health supplement food are used favorably.

Specifically, the health food is a food prepared by adding jujube extract or a fraction thereof to food materials such as beverages, teas, spices, gums, confectionery, or encapsulated, powdered, or suspension. It means bringing about an effect, but unlike general drugs, it has the advantage of having no side effects that may occur when taking the drug for a long time by using food as a raw material.

Since the food composition of the present invention can be consumed on a daily basis, a high joint protection effect can be expected and is very useful.

In addition, the composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr) may be included. In addition, amino acids such as lysine, tryptophan, cysteine, and valine may be included.

In addition, the composition includes preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching and highly bleaching, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisol (BHA), butylhydroxy Toluene (BHT), etc.), colorants (tar colors, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulsin, cyclamate, saccharin, etc.) Sodium, etc.), flavoring (vanillin, lactones, etc.), expanding agents (alum, D-potassium hydrogen stannate, etc.), reinforcing agents, emulsifying agents, thickening agents (flavoring agents), coating agents, gum base agents, foam inhibitors, solvents, improving agents, etc. (food additives). The additive may be selected according to the type of food and used in an appropriate amount.

When using the jujube extract or a fraction thereof as a food additive, the jujube extract or a fraction thereof may be added as it is, or may be used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, when ingested for a long period of time for health and hygiene purposes, the content below the above range may be included, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

As an example of the food composition of the present invention, it may be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates, etc. as an additional ingredient, such as a conventional beverage. The natural carbohydrates described above include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Polysaccharides such as dextrin and cyclodextrin; It may be a sugar alcohol such as xylitol, sorbitol, and erythritol. Sweeteners include natural sweeteners such as taumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, health beverage compositions include various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or a carbonation agent. In addition, it may contain flesh for the manufacture of natural fruit juice, fruit juice beverage, or vegetable beverage. These ingredients may be used independently or in combination. The ratio of these additives is not very important, but it is generally selected from 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The food composition of the present invention may be included in various weight percent if it can exhibit joint protection or arthritis preventing or improving effect, but specifically, a jujube extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the food composition. .

Another aspect of the present invention is to improve skin elasticity and prevent aging, including the composition for promoting production of hyaluronic acid as an active ingredient; Or it provides a cosmetic composition for preventing or improving skin wrinkles.

The term "skin elasticity" of the present invention is expressed by elastic fibers composed of elastin present in the dermal layer. These elastic fibers have a very low elastic modulus like rubber, so they are easily deformed even by small forces, and When the force is removed, it easily returns to its original shape. When hyaluronic acid, which connects the elastin and collagen, decreases, skin elasticity decreases.

The term "skin aging" of the present invention refers to symptoms such as decreased elasticity, reduced gloss, wrinkle formation, weakened regenerative power or severe dryness on the skin, which may be caused by the passage of time or external environment, and when hyaluronic acid is reduced Since skin elasticity is lowered or skin wrinkles are generated, it can be caused by a decrease in hyaluronic acid.

The term "skin wrinkles" in the present invention refers to fine lines caused by deterioration of the skin, which may be caused by genes, decrease of collagen present in the skin dermis, and external environment, and decrease in hyaluronic acid that connects elastin and collagen. When it occurs, skin wrinkles are formed.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing skin moisture content and the ability to retain moisture were very excellent (Table 7). , Since it was confirmed that the skin elasticity improvement effect was very excellent (Table 8), it can be effectively used for skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment.

The term "prevention" of the present invention means any action of inhibiting or delaying aging or skin wrinkles by administration of the composition.

The term "improvement" of the present invention means any action in which skin elasticity or skin wrinkles are improved or beneficially altered by administration of the composition of the present invention.

The cosmetic composition of the present invention may contain various weight percent if it can improve skin elasticity, prevent aging, or prevent or improve skin wrinkles, but specifically, a jujube extract or a fraction thereof is 0.00001 to 10 weight relative to the total weight of the cosmetic composition It can be included in %.

The formulation of the cosmetic composition is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, perfume, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension , Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch or spray, but is not limited thereto

The cosmetic composition may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent , Inorganic salts, pigments, antioxidants, disinfectants, preservatives, fragrances, etc. may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier varies depending on the formulation of the cosmetic composition.

When the formulation is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide are used as carrier components. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohardcarbons , Propane/butane or a propellant such as dimethyl ether may be included, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan are used. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as a carrier component. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

Another aspect of the present invention is to improve skin elasticity and prevent aging, including the composition for promoting production of hyaluronic acid as an active ingredient; Or it provides a food composition for preventing or improving skin wrinkles.

The terms "hyaluronic acid", "skin elasticity", "aging", "skin wrinkle", "prevention", "improvement" and "food" of the present invention are as described above.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing skin moisture content and the ability to retain moisture were very excellent (Table 7). , Since it was confirmed that the skin elasticity improvement effect was very excellent (Table 8), it can be effectively used for skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment.

The food composition of the present invention may contain various weight percent if it can exhibit skin elasticity improvement, aging prevention, skin wrinkle prevention or improvement effect, but specifically, a jujube extract or a fraction thereof is 0.00001 to 10% by weight based on the total weight of the food composition. Can be included as.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating skin wrinkles comprising the composition for promoting production of hyaluronic acid as an active ingredient.

The terms "hyaluronic acid", "skin wrinkles" and "prevention" of the present invention are as described above.

The term "treatment" of the present invention refers to any action in which skin wrinkles that have already occurred by the administration of the composition are improved or beneficial.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing skin moisture content and the ability to retain moisture were very excellent (Table 7). , Since it was confirmed that the skin elasticity improvement effect was very excellent (Table 8), it can be effectively used for skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment.

The pharmaceutical composition of the present invention may be included in various weight% if it can exhibit skin wrinkle prevention or treatment effect, but specifically, it may contain a jujube extract or a fraction thereof in an amount of 0.00001 to 10% by weight relative to the total weight of the pharmaceutical composition. .

Another aspect of the present invention provides a cosmetic composition for moisturizing skin comprising the composition for promoting production of hyaluronic acid as an active ingredient.

The formulation and carrier of the hyaluronic acid and cosmetic composition of the present invention are as described above.

The term "moisturizing" of the present invention means maintaining the homeostasis of the living body by appropriately controlling moisture loss (water evaporation), etc., and moisture is lost when hyaluronic acid in the skin decreases, and moisture is increased when hyaluronic acid is increased. can do.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increasing effect and the water retention ability were very excellent (Table 7). , It can be used effectively for skin moisturizing.

The cosmetic composition of the present invention may be included in various weight percent if it can exhibit a skin moisturizing effect, but specifically, the jujube extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight based on the total weight of the cosmetic composition.

Another aspect of the present invention provides a food composition for moisturizing skin comprising the composition for promoting production of hyaluronic acid as an active ingredient.

The "hyaluronic acid", "moisturizing" and "food" of the present invention are as described above.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increasing effect and the water retention ability were very excellent (Table 7). , It can be used effectively for skin moisturizing.

The food composition of the present invention may be included in various weight percent if it can exhibit a skin moisturizing effect, but specifically, a jujube extract or a fraction thereof may be included in an amount of 0.00001 to 10 weight percent based on the total weight of the food composition.

Another aspect of the present invention provides a quasi-drug composition for skin moisturizing comprising the composition for promoting production of hyaluronic acid as an active ingredient.

The "hyaluronic acid", "moisturizing" and "quasi drug" of the present invention are as described above.

In an embodiment of the present invention, it was confirmed that the jujube extract can increase the hyaluronic acid concentration in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increasing effect and the water retention ability were very excellent (Table 7). , It can be used effectively for skin moisturizing.

The quasi-drug composition of the present invention may be included in various weight percent if it can exhibit a skin moisturizing effect, but specifically, a jujube extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the quasi-drug composition.

Since the jujube extract or its fraction effectively promotes the production of hyaluronic acid and the expression of hyaluronic acid synthase, it can be usefully used for joint protection, arthritis prevention or treatment, skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment, and skin moisturization. have.

Hereinafter, in order to describe the present invention in more detail, it will be described with reference to the following examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided by way of example to aid in a detailed understanding of the present invention.

Manufacturing example 1. Preparation of Jujube Extract

The jujube extract used in the following examples was an ethanol extract, which was purchased and used from OBM Lab.

Example 1. Evaluation of cytotoxicity of jujube extract

Cell Counting Kit-8 (Dojindo Lab, USA) for cell division changes in human fibroblast-like synoviocytes (HFLS-OA, Cell Applications) collected from patients with degenerative arthritis to evaluate the cytotoxicity of jujube extract. It was measured using.

First, after spreading a uniform number of cells on a 96 well plate and stabilizing, the jujube extract was treated to 5 or 10 ppm, followed by additional culture for 24 hours. After 2 hours after treatment with CCK-8, absorbance was measured at 450 nm. The relative cell viability of cells treated with jujube extract compared to the untreated group (control group) was analyzed as follows, and the results are shown in Table 1 below.

sample Untreated group Glucosamine
1000 ppm Jujube extract
5 ppm Jujube extract
10 ppm Survival rate (%) 100 97 94 95

* Number of repetitions = 3

* Relative cell viability = (extract treatment group)/(no treatment group) X 100

As shown in Table 1, it was found that the number of cells did not change significantly by the jujube extract treatment. This suggests that the jujube extract does not cause cytotoxicity.

Example 2. Patients with degenerative arthritis In synovial cells Hyaluronic acid concentration increase by jujube extract

Human fibroblast-like synovial cells derived from patients with degenerative arthritis were cultured with Synoviocyte Growth Medium (Cell Applications), and were dispensed into 96-well plates at 2.0×10 ³ cells per well. After culturing for 3 days, 200 µl of human periosteal cell growth medium was exchanged, and jujube extract was added at different concentrations (5 and 10 ppm). The same amount of ethanol was treated as a negative control, and 1000 ppm of glucosamine hydrochloride (Sigma-aldrich), known to promote hyaluronic acid production, was treated as a positive control. After culturing for 24 hours, the cell culture solution was recovered and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), as shown in Table 2.

sample No treatment
(Negative control) Glucosamine
1000 ppm Jujube extract
5 ppm Jujube extract
10 ppm Hyaluronic acid
Relative concentration (%) 100 146 147 203

* Number of repetitions = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with the jujube extract was quantified.

As a result, jujube extract increased hyaluronic acid production in synovial cells of patients with degenerative arthritis in a concentration-dependent manner. In addition, the jujube extract showed a higher hyaluronic acid concentration even in an amount 200 times less than that of the positive control.

Through this, it has been confirmed that the jujube extract has a very excellent effect of promoting hyaluronic acid production in synovial cells of patients with degenerative arthritis, and thus can be usefully used for joint protection or arthritis prevention or treatment.

Example 3. Patients with degenerative arthritis In synovial cells Hyaluronic acid synthase from jujube extract ( HAS ) Increased gene expression

Human fibroblast-like synovial cells derived from degenerative arthritis patients were dispensed with human periosteal cell proliferation medium into 6-well plates and cultured for 3 days, and then the medium was exchanged and 10ppm jujube extract was treated for 24 hours. To recover the cells, the extraction of the total RNA after, quantified by RNeasy mini kit (Qiagen) was used for the RNA of ^{1㎍ GeneAmp ® RNA PCR kit (Applied} Biosystems) RT (reverse transcription). The reverse transcription reaction was performed using a Mycycler ^® PCR instrument (Biorad).

Subsequently, quantitative real-time PCR was performed using 2 μl of reverse transcription reaction solution and Taqman ^® probe (Invitrogen, USA, Hs00193435_m1, Hs00193436_m1) of HAS-2, HAS-3, and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase). Real time PCR was performed using a CFX96 Touch ^TM Real-Time PCR Detection System instrument (Biorad). Experimental results obtained through real time PCR were calculated and shown by the method described in Livak KJ et al. (Methods, 2001, 25, 402-408) based on GAPDH, a housekeeping gene.

In addition, the mRNA expression level of the experimental group treated with jujube extract was quantified and shown in Table 3 using the mRNA expression level of the negative control group as a standard (100%).

sample No treatment
(Negative control) Glucosamine
1000 ppm Jujube extract
5 ppm Jujube extract
10 ppm HAS-2
Expression amount (%) 100 113 121 127 HAS-3
Expression amount (%) 100 121 113 132

* Number of repetitions = 4

As a result, the expression of hyaluronic acid synthase, especially HAS-2 and HAS-3, was increased in synovial cells of patients with degenerative arthritis by treatment with jujube extract. In addition, the jujube extract showed the expression levels of HAS-2 and HAS-3 similar to the positive control even at 200 times less than the positive control.

Through this, it was confirmed that the jujube extract has a very excellent effect of increasing the expression of hyaluronic acid synthase in synovial cells of patients with degenerative arthritis, and thus can be usefully used for joint protection or arthritis prevention or treatment.

Example 4. Normal In synovial cells Hyaluronic acid concentration increase by jujube extract

Human fibroblast-like synovial cells were cultured with Synoviocyte Growth Medium (Cell Applications), and were dispensed into a 96-well plate at 2.0×10 ³ cells per well. After culturing for 3 days, 200 µl of human periosteal cell growth medium was exchanged, and 10 ppm of jujube extract was added. The same amount of solvent was treated as a negative control, and 1000 ppm of glucosamine hydrochloride was treated as a positive control. After culturing for 24 hours, the cell culture solution was recovered, and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems) and shown in Table 4.

sample No treatment
(Negative control) Glucosamine
1000 ppm Jujube extract
10 ppm Hyaluronic acid
Relative concentration (%) 100 146 129

* Number of repetitions = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with the jujube extract was quantified.

As a result, the jujube extract increased hyaluronic acid production in normal synovial cells. In addition, the jujube extract showed a hyaluronic acid concentration similar to that of the positive control even in an amount 100 times less than that of the positive control.

Through this, it was confirmed that the jujube extract has a very excellent effect of promoting hyaluronic acid production in normal synovial cells, and thus can be usefully used for joint protection or arthritis prevention or treatment.

Example 5. Rat In the joint cavity Hyaluronic acid concentration increase by jujube extract

After purchasing a male Sprague-Dawley Rat (Samtaco, Korea) with a weight of about 200g and acclimating it for one week in a thermo-hygrostat (Daejong Machine, Korea) adjusted to a temperature of 23±1℃ and a humidity of 55±5%. It was used in the experiment. To the sample treatment group (n=8), jujube extract was diluted in physiological saline, and 100 or 500 mg per 1 kg of rat body weight was orally administered once a day for 15 days, and only the solvent was administered in the same manner for the control group (n=8). I did. Thereafter, the femur and tibia were removed together to measure the hyaluronic acid concentration, and then 0.5 ml of physiological saline was injected into the left knee joint for washing, and 100 μl or more of the washed synovial fluid was collected and stored frozen at -70° C. before examination. The hyaluronic acid concentration in the synovial fluid was measured by Hyaluronan ELISA kit (R&D Systems) and shown in Table 5.

Control Sample treatment group 1 serving of jujube - 100mg/kg 500mg/kg Hyaluronic acid
Relative concentration (%) 100 147 168

* The hyaluronic acid concentration of the sample treatment group is quantified based on the hyaluronic acid concentration of the control group (100%).

As a result, jujube extract increased the production of hyaluronic acid in the joint cavity of normal rats.

Through this, the jujube extract is in It has been confirmed that the effect of promoting hyaluronic acid production in vivo in the rat joint cavity is very excellent, and it can be usefully used for joint protection or arthritis prevention or treatment.

Example 6. Increased hyaluronic acid concentration by jujube extract in HaCaT cells

Human-derived keratinocytes, HaCaT cells, were prepared at 2×10 ⁴ cells/ml in DMEM medium containing 10% FBS, and 1 ml each was dispensed into a 24-well plate. After culturing for 24 hours, it was exchanged with 1 ml of DMEM medium not containing bovine serum, and jujube extract was added at 30 ppm. An equal amount of ethanol was treated as a negative control, and 100 ppm of glucosamine hydrochloride (Sigma-aldrich), which is known to promote hyaluronic acid production, was treated as a positive control. After culturing for 48 hours, the cell culture solution was recovered, and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), as shown in Table 6.

sample No treatment
(Negative control) Glucosamine
100 ppm Jujube extract
30 ppm Hyaluronic acid
Relative concentration (%) 100 199 256

* Number of repetitions = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with the jujube extract was quantified.

As a result, the jujube extract increased hyaluronic acid production in human keratinocytes.

Through this, it was confirmed that the jujube extract has a very excellent effect of promoting hyaluronic acid production in human keratinocytes, improving skin elasticity, preventing aging, preventing or treating skin wrinkles; And it can be usefully used for skin moisturizing.

Example 7. Increased skin moisture content by jujube extract

In order to apply the effect of increasing skin moisture content to the cosmetic composition containing jujube extract to human skin, it was prepared according to the composition of the nutritional cream of Formulation Example 2 below, and the lower chest of 30 healthy adults in a constant temperature and humidity room at 22°C and 50% relative humidity. A certain amount (2 mg/cm2) was applied inside, and the moisture content of the skin was measured before application, 1 hour after application, and 2 hours after application. In the case of the control group, a nutritional cream containing the same amount of ethanol was prepared and applied in the same manner instead of jujube extract, and normal skin without any treatment was used as the untreated group. The water retention ability was measured using a Corneometer (Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the skin surface. The results are shown in Table 7 below.

Corneometer units Formulation Example 2
Nourishing cream Control
Nourishing cream Untreated group Before application 57 54 56 1 hour after application 100 79 55 2 hours after application 79 65 52

* n=30

As a result, the nutritional cream containing jujube extract increased the amount of moisture in human skin, and showed excellent moisture retention even after 2 hours of application.

Through this, it was confirmed that the jujube extract has a very excellent effect of increasing skin moisture content and retaining moisture, improving skin elasticity, preventing aging, preventing or treating skin wrinkles; And it can be usefully used for skin moisturizing.

Example 8. Improvement of skin elasticity by jujube extract

In order to apply the effect of enhancing skin elasticity to the cosmetic composition containing the jujube extract of the present invention to human skin, it was prepared according to the composition of the nutritional cream of Formulation Example 2 below, and healthy adults 30 in a constant temperature and humidity room at 22°C and 50% relative humidity. The skin elasticity improvement effect was compared and evaluated by measuring the elasticity before and after the chart after applying it to the neck of a person for 4 weeks.

A nutritional cream containing jujube extract was used on the left side of the subject's neck, and a nutritional cream containing the same amount of ethanol instead of jujube extract was used twice a day for 4 weeks after washing in the morning and evening. Thereafter, the degree of improvement of the elasticity in each area was measured using a skin elasticity meter (Cutometer, C+K, Germany). As for the degree of improvement, Ur/Uf was used as a parameter, and the results are shown in Table 8.

Formulation Example 2
Nourishing cream Control
Nourishing cream Elasticity improvement rate (%) 28.14 4.85

* n=30

As a result, the nutritional cream containing jujube extract improved the elasticity of human skin and did not show any side effects in the skin.

Through this, it was confirmed that the jujube extract has a very excellent effect of improving skin elasticity, and thus it can be usefully used for improving skin elasticity, preventing aging, and preventing or treating skin wrinkles.

Based on the results of the effect test as described above, various formulation examples of the composition according to the present invention will be described in detail. However, these formulation examples are only examples of the composition of the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the composition of the present invention is not limited to these formulations.

Formulation example One. Health drink Produce

0.1 mg jujube extract

Citric acid 1000 mg

100 g oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 mL with purified water

After mixing the above ingredients according to the normal health drink manufacturing method, stirring and heating at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L-container, sealed and sterilized, and then stored in a refrigerator. It was used to prepare the health beverage composition of the invention.

The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences, such as the demand class, the country of demand, and the intended use.

Formulation example 2. Manufacture of nourishing cream

As shown in the following composition, a nutritional cream containing jujube extract as an active ingredient was prepared according to a conventional method.

1.0% by weight of jujube extract

5.0% by weight of beta-1,3-glucan

10.0 wt% beeswax

1.5% by weight of polysorbate 60

Fiji 60 hydrogenated castor oil 2.0% by weight

0.5% by weight of sorbitansquioleate

Liquid paraffin 10.0% by weight

5.0% by weight of squalane

Caprylic/Capric Triglyceride 5.0% by weight

5.0% by weight of glycerin

Butylene glycol 3.0% by weight

Propylene glycol 3.0% by weight

0.2% by weight of triethanolamine

0.05% by weight of preservative

0.05% by weight of pigment

Perfume 0.05% by weight

Purified water to 100% by weight

Formulation example 3: Preparation of drugs for topical administration (gel ointment)

As shown in the following composition, a gel ointment containing jujube extract as an active ingredient was prepared according to a conventional method.

0.5% by weight of jujube extract

Beta-1,3-glucan 10.0% by weight

1.5% by weight of polyacrylic acid (Carbopol 940)

5.0% by weight of isopropanol

25.0% by weight of hexylene glycol

1.7 wt% triethanolamine

Deionized water to 100% by weight

Formulation example 4: Preparation of granules

2 mg jujube extract

Soybean extract 50 mg

200 mg of glucose

Starch 600 mg

After mixing the above ingredients, 100 mg of 30% ethanol was added and dried at 60° C. to form granules, and then filled into a cloth.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof.

Claims (6)

A food composition for joint protection, comprising a jujube extract as an active ingredient, wherein the joint protection is by promoting hyaluronic acid production.
A quasi-drug composition for joint protection comprising a jujube extract as an active ingredient, wherein the joint protection is by promoting hyaluronic acid production, a quasi-drug composition for joint protection.
delete delete delete The food composition of claim 1, wherein the composition comprises 0.00001 to 10% by weight based on the total weight of the composition.