KR102357341B1 - Composition for promoting synthesis of hyaluronic acid comprising Rehmanniae Radix Preparata extracts and the use thereof - Google Patents

Abstract

Since Sukhumus extract or a fraction thereof effectively promotes hyaluronic acid production and expression of hyaluronic acid synthase, it can be usefully used to protect joints, prevent or treat arthritis, improve skin elasticity, prevent aging, prevent or treat skin wrinkles, and moisturize the skin. have.

Description

Composition for promoting synthesis of hyaluronic acid comprising Rehmanniae Radix Preparata extracts and the use thereof

The present invention relates to a composition for promoting hyaluronic acid production, comprising an extract of Rehmanniae Radix Preparata or a fraction thereof as an active ingredient.

In addition, the present invention relates to a pharmaceutical composition for joint protection or arthritis prevention or treatment comprising the composition as an active ingredient, a quasi-drug composition for joint protection or arthritis prevention or improvement, or a food composition.

In addition, the present invention is a cosmetic composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, or a food composition comprising the composition for promoting hyaluronic acid production as an active ingredient; Or it relates to a pharmaceutical composition for preventing or treating skin wrinkles.

In addition, the present invention relates to a cosmetic composition for skin moisturizing, a food composition or a quasi-drug composition comprising the composition for promoting hyaluronic acid production as an active ingredient.

Hyaluronic acid (hyaluronic acid) is a type of glycosaminoglycans (glycosaminoglycans), glucuronic acid and N-acetyl glucosamine residues are repeatedly linked to the chain-shaped polymer polysaccharide material. It combines with a large amount of water to form a gel, so it has high viscosity and elasticity. As a major component of the extracellular matrix, hyaluronic acid is reported to be involved not only in water retention, maintenance of intercellular spacing, storage and diffusion of cell growth factors and nutrients, but also in cell division, differentiation, and migration. have.

In the joint, hyaluronic acid is secreted from fibrolast-like synoviocytes as a major component of the synovial fluid that protects the joint. Hyaluronic acid acts to lubricate the joints, and a decrease in hyaluronic acid is one of the causes of osteoarthritis. As the hyaluronic acid in the joint decreases, the joint cannot absorb or disperse external shocks, which can lead to severe joint damage.

The concentration of hyaluronic acid in synovial fluid is determined by hyaluronic acid production by hyaluronic acid synthase (HAS) and hyaluronic acid degradation by hyaluronic acid degrading enzyme (Hyaluronidase, HYAL). In patients with degenerative arthritis and rheumatoid arthritis, the expression of HAS-1 and HAS-2 in the synovial membrane is decreased, and the expression of HYAL-2 is increased (Arthritis Res Ther, 2004, 6, R514-R520).

On the other hand, in the skin, it has been reported that more than 50% of hyaluronic acid present in the body of mammals is distributed in the skin, particularly the intercellular gap of the epidermis and the connective tissue of the dermis. is known to be It has been reported that the amount of hyaluronic acid in human skin decreases with aging, and the decrease in hyaluronic acid in the skin is considered to be one of the direct causes of the decrease in skin elasticity and moisture content with aging (Biochem Biophys Acta 279, 265-275). ; Carbohydr Res 159, 127-136; Int J Dermatol 33, 119-122). Also, hyaluronic acid is known to be involved in maintaining the structure of the stratum corneum and maintaining the skin barrier function (J Cosmet Dermatol. 2007 Jun, 6(2), 75-82).

However, hyaluronic acid having the above effects is difficult to ingest with food and poor absorption. In addition, the method of relieving arthritis by injecting hyaluronic acid into the joint was approved by the US FDA in 1997 and is currently being operated. However, in order to ultimately solve arthritis, a method of increasing hyaluronic acid synthesis in the body is more effective. Therefore, research on methods to increase the production of hyaluronic acid in the human body is being actively conducted, but remarkable research results are not yet known.

Under this background, the present inventors made efforts to develop a substance capable of increasing the synthesis of hyaluronic acid in the body, and as a result, a composition including an extract of Rehmanniae Radix Preparata or a fraction thereof promotes the production of hyaluronic acid and synthesizes hyaluronic acid By increasing the expression of enzyme (HAS), joint protection, arthritis prevention or treatment, skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment; And it was confirmed that there is a skin moisturizing effect, and completed the present invention.

One object of the present invention is to provide a composition for promoting hyaluronic acid production, comprising an extract of Rehmanniae Radix Preparata or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a composition for joint protection or arthritis prevention or treatment comprising the composition as an active ingredient.

Another object of the present invention is to provide a composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, comprising the composition for promoting hyaluronic acid production as an active ingredient.

Another object of the present invention is to provide a skin moisturizing composition comprising the composition for promoting hyaluronic acid production as an active ingredient.

Another object of the present invention is to provide a composition wherein the composition is used as any one selected from the group consisting of a cosmetic composition, a food composition, a pharmaceutical composition and a quasi-drug composition.

Another object of the present invention is to provide a composition wherein the composition comprises 0.00001 to 10% by weight of the sukjihwang extract or a fraction thereof based on the total weight of the composition.

One object of the present invention is to provide a composition for promoting hyaluronic acid production, comprising an extract of Rehmanniae Radix Preparata or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for joint protection or arthritis prevention or treatment comprising the composition as an active ingredient, a quasi-drug composition for joint protection or arthritis prevention or improvement, or a food composition.

Another object of the present invention is a cosmetic composition for improving skin elasticity, preventing aging, or preventing or improving skin wrinkles, or a food composition comprising the composition for promoting hyaluronic acid production as an active ingredient; Or to provide a pharmaceutical composition for preventing or treating skin wrinkles.

Another object of the present invention is to provide a cosmetic composition for skin moisturizing, a food composition or a quasi-drug composition comprising the composition for promoting hyaluronic acid production as an active ingredient.

In order to achieve the above object, one aspect of the present invention provides a composition for promoting hyaluronic acid production comprising an extract of Rehmanniae Radix Preparata or a fraction thereof as an active ingredient.

As used herein, the term " Sukjihwang ( Rehmanniae Radix Preparata)" is glutinosa Liboschitz ex Steudel), which has been processed into cloth, is known to have effects such as strengthening immune and heart functions, protecting the liver, lowering blood sugar, and smoothing diuresis.

Sukjihwang of the present invention can be purchased commercially sold, or grown or harvested in nature can be used.

As used herein, the term "extract" refers to an extract obtained by the extraction treatment of Sulfuricaceae, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, such as the extract itself. and extracts of all formulations that can be formed using the extract. Specifically, the extract of the present invention may be prepared and used in the form of a dry powder after extraction.

The extract may be extracted from a natural, hybrid or mutated plant of the plant, and may also be extracted from a plant tissue culture.

The preparation method of the Sukhumi extract is not particularly limited, and it may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, and reflux extraction, and these may be performed alone or in combination of two or more methods. In addition, in order to obtain a high-purity extract, the extract may be further extracted one or more times in the same manner.

The type of the extraction solvent used for the preparation of the Sukhumus extract is not particularly limited, and any solvent known in the art may be used as long as an extract having the desired effect of the present invention can be obtained. For example, one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, or chloroform may be used. More specifically, ethanol may be used as a solvent.

As used herein, the term "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific group of components from a mixture containing various components.

The fractionation method for obtaining the fraction is not particularly limited, and as long as a fraction having the desired effect of the present invention can be obtained, it may be carried out according to a method commonly used in the art. A non-limiting example of the fractionation method may include a method of obtaining a fraction from the extract by treating a predetermined fractionation solvent to an extract obtained by adding a solvent to the sukjihwang extract.

The type of the fractionation solvent is not particularly limited, and any solvent known in the art may be used as long as a fraction having the desired effect of the present invention can be obtained. Non-limiting examples of the fractionation solvent include organic solvents such as water, alcohol, ethyl acetate, acetone or chloroform. These may be used alone or in combination of two or more. Specifically, the fractionation solvent may be at least one selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, and chloroform, but is not limited thereto.

As used herein, the term "hyaluronic acid" is a type of glycosaminoglycans and refers to a chain-shaped polymeric polysaccharide material in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked.

In one embodiment of the present invention, the Sukhumi extract increases the production of hyaluronic acid in a concentration-dependent manner in human fibroblast-like synovial cells derived from a degenerative arthritis patient, and similar hyaluronic acid is also used in an amount 100 to 200 times lower than that of glucosamine (positive control). It was confirmed that the synthesis efficiency was shown (Table 2).

In addition, in human fibroblast-like synovial cells derived from a degenerative arthritis patient, Sukhumi Hwang extract increased the expression of hyaluronic acid synthetase, especially HAS-2 and HAS-3, and was similar to the positive control group at an amount 200 times lower than that of glucosamine. HAS-2 expression level was shown, and it was confirmed that the expression level of HAS-3 was higher (Table 3).

In addition, in normal human-derived fibroblast-like synovial cells, it was confirmed that the Sukhumvitae extract increased the production of hyaluronic acid, and showed a similar hyaluronic acid concentration even at an amount 100 times less than that of glucosamine (Table 4).

In addition, the Sukhumi hwang extract increased the production of hyaluronic acid even in the rat joint cavity (Table 5). Through this, the extract of Sukhumia extract is not only used in synovial cells but also in It was confirmed that it exhibited an excellent hyaluronic acid production promoting effect in the rat joint cavity in vivo .

Another aspect of the present invention provides a pharmaceutical composition for joint protection or arthritis prevention or treatment comprising the composition as an active ingredient. The pharmaceutical composition may increase the expression of hyaluronic acid synthase (HAS).

The term "joint protection" of the present invention means to prevent joint damage due to various causes, and since hyaluronic acid is a major component of synovial fluid that protects joints, when hyaluronic acid is reduced, external shocks are absorbed in the joint Failure to do so may result in joint damage or osteoarthritis.

As used herein, the term “arthritis” refers to inflammation in the joints due to various causes, and when hyaluronic acid is reduced, arthritis may occur. Specifically, the arthritis is osteoarthritis, degenerative arthritis, rheumatoid arthritis, spondyloarthropathy, ankylosing spondylitis, psoriatic arthritis, gout, bacterial arthritis, childhood rheumatoid arthritis, lupus, scleroderma, multiple sclerosis, fibromyalgia, polymyositis, dermatomyositis, Behcet's disease, Reiter's syndrome, Lyme arthritis, adhesive capsulitis, frozen shoulder, tendon synovitis, elbow capsulitis, de Quervain's tendon synovitis, recurrent rheumatism, polymyalgia rheumatica or adult-type Still's disease, more specifically degenerative arthritis or rheumatoid arthritis can, but is not limited thereto.

In an embodiment of the present invention, the Sukhumihuang extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity. It was confirmed that it is possible to increase the hyaluronic acid concentration in (Tables 2 to 5). Hyaluronic acid lubricates joints, and as it is known that osteoarthritis can occur when hyaluronic acid is reduced, Sukhumihuang extract or a fraction thereof, which has an excellent effect of promoting hyaluronic acid production, can be effectively used to protect joints or prevent or treat arthritis.

The pharmaceutical composition of the present invention may contain various weight % as long as it can exhibit joint protection or arthritis prevention or treatment effects, but specifically, Sukhumi Hwang extract or a fraction thereof is included in an amount of 0.00001 to 10% by weight relative to the total weight of the pharmaceutical composition. can

As used herein, the term “prevention” refers to any action of inhibiting or delaying the onset of arthritis by administering the composition.

As used herein, the term “treatment” refers to any action that improves or benefits the symptoms of arthritis that have already occurred by administering the composition.

In addition, the pharmaceutical composition of the present invention may further include an appropriate carrier, excipient or diluent according to a conventional method. Carriers, excipients and diluents that may be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

The pharmaceutical composition according to the present invention may be formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods. have. Specifically, it can be prepared using a diluent or excipient such as a filler, extender, binder, wetting agent, disintegrant, surfactant, etc. usually used when formulating. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. to the compound. It can be prepared by mixing. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral administration include suspensions, internal solutions, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives are included. can Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, Witepsol, Macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, etc. can be used.

The composition of the present invention is administered in a pharmaceutically effective amount. The pharmaceutically effective amount means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects, and the effective dose level is determined by the patient's health condition, disease type, severity, The activity of the drug, the sensitivity to the drug, the administration method, administration time, administration route and excretion rate, treatment period, factors including drugs used in combination or concurrently, and other factors well known in the medical field may be determined according to factors. Specifically, it is generally 0.01 mg to 5000 mg per 1 kg of body weight of the administered subject, and may be administered in divided doses once or several times a day at regular time intervals according to the judgment of a doctor or pharmacist, but is not limited thereto.

In one embodiment of the present invention, 100 or 500 mg per 1 kg of rat body weight was orally administered once a day for 15 days by diluting the extract of Sukhumi Hwangseok in physiological saline to male Sprague-Dawley Rat (Samtaco, Korea) weighing about 200 g. , It was confirmed that the extract according to the present invention increases the production of hyaluronic acid in the joint cavity of normal rats (Example 5).

In addition, the pharmaceutical composition of the present invention alone or joint protection; Alternatively, it may be used in combination with other pharmaceutically active compounds exhibiting an arthritic preventive or therapeutic effect or in an appropriate group.

In addition, the pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans by various routes, any pharmaceutical composition of the present invention in which an active substance can migrate to a target cell. The administration means providing a predetermined substance to the patient by any suitable method, and the administration route of the composition of the present invention is not limited as long as it can reach the target tissue. For example, intra-articular administration, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, intrapulmonary administration, may be administered rectally, but is not limited thereto. In addition, the pharmaceutical composition of the present invention may be administered by any device capable of transporting an active substance to a target cell.

Another aspect of the present invention provides a method for preventing or treating arthritis comprising administering to an individual the composition for promoting hyaluronic acid production.

The terms "hyaluronic acid", "arthritis", "prevention", "treatment" and "administration" of the present invention are as described above.

The dosage, administration frequency, and administration route for the administration are the same as described above.

As used herein, the term "individual" refers to monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, rats, rabbits or guinea pigs, including humans, who have or can develop the arthritis. It means all animals, including, and can effectively prevent or treat the disease by administering the composition of the present invention to an individual.

Another aspect of the present invention provides a quasi-drug composition for joint protection or arthritis prevention or improvement comprising the composition for promoting hyaluronic acid production as an active ingredient.

In an embodiment of the present invention, the Sukhumihuang extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity. Since it was confirmed that it is possible to increase the concentration of hyaluronic acid in (Tables 2 to 5), the composition containing the Sukhumi extract or fraction can be effectively used to protect joints or prevent or improve arthritis.

As used herein, the terms "hyaluronic acid", "arthritis" and "prevention" are the same as described above, and "improvement" refers to any action in which the symptoms of arthritis are improved or beneficially changed by administration of the composition of the present invention.

As used herein, the term "quasi-drug" refers to fibers, rubber products, or the like, which are used for the purpose of treating, alleviating, treating or preventing diseases of humans or animals, have weak action on the human body, or do not act directly on the human body, Non-machines and similar products, which are products that are used for sterilization, insecticide, and similar purposes to prevent infection Items that are not instruments, machines, or devices, and items used for the purpose of pharmacologically affecting the structure and function of humans or animals, other than those that are not instruments, machines, or devices It may be a hygiene product, but is not limited thereto.

When the Sukhumus extract or fraction is added to a quasi-drug composition for the purpose of joint protection or arthritis prevention or improvement, the Sukhumvitae extract or fraction can be added as it is or used together with other quasi-drug ingredients, and can be used appropriately according to a conventional method. . The mixing amount of the active ingredient may be appropriately determined according to the purpose of use.

The external preparation for skin is not particularly limited thereto, but may be prepared and used in the form of, for example, ointment, lotion, spray, patch, cream, powder, suspension, gel, or gel. The personal care products are not particularly limited thereto, but specifically, soap, cosmetics, wet wipes, toilet paper, shampoo, skin cream, face cream, toothpaste, lipstick, perfume, makeup, foundation, ball touch, mascara, eye shadow, sunscreen It may be a lotion, a hair care product, an air freshener gel or a cleansing gel. In addition, another example of the quasi-drug composition of the present invention is a disinfectant cleaner, shower foam, wet tissue, detergent soap, hand wash, mask or ointment.

The quasi-drug composition of the present invention may contain various weight % as long as it can exhibit joint protection or arthritis prevention or improvement effects, but specifically sukhumi extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the quasi-drug composition. .

Another aspect of the present invention provides a food composition for joint protection or arthritis prevention or improvement comprising the composition for promoting hyaluronic acid production as an active ingredient. Since Sukjihwang has been used as a natural material for a long time and its stability has been proven, it can be prepared and consumed in the form of food that can protect joints or prevent or improve arthritis while being common knowledge.

In an embodiment of the present invention, the Sukhumihuang extract can increase the hyaluronic acid concentration and the mRNA expression level of the hyaluronic acid synthase (HAS) gene in human fibroblast-like synovial cells derived from degenerative arthritis patients, and in normal synovial cells and rat joint cavity. Since it was confirmed that it is possible to increase the concentration of hyaluronic acid in (Tables 2 to 5), the composition containing the Sukhumi extract or fraction can be effectively used to protect joints or prevent or improve arthritis.

The terms "hyaluronic acid", "arthritis", "prevention" and "improvement" of the present invention are as described above.

As used herein, the term “food” refers to meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, There are vitamin complexes, functional foods, and health foods, and includes all foods in a conventional sense.

The functional food (functional food) is the same term as food for special health use (FoSHU), and refers to food with high medical and medical effects processed to efficiently exhibit bioregulatory functions in addition to nutrition supply. . Here, "functional" means to obtain useful effects for health purposes, such as regulating nutrients or physiological actions with respect to the structure and function of the human body. The functional food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and components commonly added in the art. In addition, the formulation of the functional food may be prepared without limitation as long as it is a formulation recognized as a functional food. The composition for food of the present invention can be prepared in various forms, and unlike general drugs, it has the advantage that there are no side effects that may occur during long-term administration of the drug using food as a raw material, and it is excellent in portability, and the present invention Functional foods can be ingested as a supplement to enhance the effect of preventing or improving arthritis.

The health food means a food having an active health maintenance or promotion effect compared to general food, and the health supplement food means a food for the purpose of health supplementation. In some cases, the terms functional food, health food, and health supplement may be used interchangeably.

Specifically, the health food is a food prepared by adding or encapsulating, powdering, or suspension to food materials such as beverages, teas, spices, gums, and confectionery obtained by adding S. Sukhumus extract or a fraction thereof, and when ingested, It means to bring an effect, but unlike general drugs, it has the advantage that there are no side effects that may occur when taking the drug for a long time using food as a raw material.

Since the food composition of the present invention can be ingested on a daily basis, a high joint protective effect can be expected and is very useful.

In addition, the composition may include additional ingredients that are commonly used in food compositions to improve odor, taste, vision, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, pantothenic acid, and the like may be included. Also, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr). In addition, it may include amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the composition includes a preservative (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), a disinfectant (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), an antioxidant (butylhydroxyanisole (BHA), butylhydroxy toluene (BHT), etc.), coloring agents (tar pigments, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclamate, saccharin, Sodium, etc.), flavorings (vanillin, lactones, etc.), swelling agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (flavors), film agents, gum base agents, foam inhibitors, solvents, food additives such as improving agents (food additives). The additive may be selected according to the type of food and used in an appropriate amount.

In the case of using the Sukhumus extract or its fraction as a food additive, it may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, in the production of food or beverage, the food composition of the present invention may be added in an amount of 15 parts by weight or less, specifically 10 parts by weight or less, based on the raw material. However, when ingested for a long period of time for health and hygiene purposes, it may contain an amount below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

As an example of the food composition of the present invention, it may be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates as an additional component like a conventional drink. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as taumatine, stevia extract; A synthetic sweetener such as saccharin or aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the health drink composition includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid, pectic acid salts, alginic acid, alginic acid salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol, a carbonation agent, or the like. In addition, it may contain the pulp for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These components may be used independently or in combination. Although the proportion of these additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The food composition of the present invention may contain various weight % as long as it can exhibit joint protection or arthritis prevention or improvement effects, but specifically, sukhumi hwang extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the food composition. .

Another aspect of the present invention is to improve skin elasticity and prevent aging, comprising the composition for promoting hyaluronic acid production as an active ingredient; Or it provides a cosmetic composition for preventing or improving skin wrinkles.

The term "skin elasticity" of the present invention is represented by elastic fibers composed of elastin present in the dermal layer, and these elastic fibers have a very low elastic modulus like rubber, so they are easily deformed by a small force, and When the force is removed, it easily returns to its original shape. When hyaluronic acid, which connects the elastin and collagen, is reduced, skin elasticity is reduced.

As used herein, the term "skin aging" refers to the appearance of symptoms such as reduced elasticity, reduced gloss, wrinkles, weakened regeneration, or severe dryness in the skin, and may be induced by the passage of time or external environment, and when hyaluronic acid is reduced It can be caused by a decrease in hyaluronic acid because skin elasticity is reduced or skin wrinkles are generated.

As used herein, the term "skin wrinkle" refers to fine lines caused by skin aging, which can be caused by genetic causes, a decrease in collagen present in the dermis of the skin, external environment, etc., and a decrease in hyaluronic acid connecting elastin and collagen This creates wrinkles on the skin.

In an embodiment of the present invention, it was confirmed that the Sukhumi extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increase effect and moisture retention ability were very excellent (Table 7) , since it was confirmed that the skin elasticity improvement effect is very excellent (Table 8), it can be effectively used for skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment.

As used herein, the term “prevention” refers to any action that inhibits or delays aging or skin wrinkles by administering the composition.

As used herein, the term “improvement” refers to any action in which skin elasticity or skin wrinkles are improved or beneficially changed by administration of the composition of the present invention.

The cosmetic composition of the present invention may include various weight % as long as it can improve skin elasticity, prevent aging, or prevent or improve skin wrinkles, but specifically, sukhumihuang extract or a fraction thereof from 0.00001 to 10 weights relative to the total weight of the cosmetic composition % can be included.

The formulation of the cosmetic composition is a solution, an external ointment, a cream, a foam, a nutritional lotion, a flexible lotion, a perfume, a pack, a soft water, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bath agent, a sunscreen cream, a sun oil, a suspension , emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch or spray.

The cosmetic composition may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent , inorganic salts, colorants, antioxidants, bactericides, preservatives, fragrances, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier varies depending on the formulation of the cosmetic composition.

When the formulation is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. are used as carrier components. may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon , propane/butane or propellants such as dimethyl ether, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol. , 1,3-butylglycol oil, etc. can be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan is used. may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation is a suspension, as the carrier component water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. are used as carrier components may be, but is not limited thereto. These may be used alone or in combination of two or more.

Another aspect of the present invention is to improve skin elasticity and prevent aging, comprising the composition for promoting hyaluronic acid production as an active ingredient; Or it provides a food composition for preventing or improving skin wrinkles.

The terms "hyaluronic acid", "skin elasticity", "aging", "skin wrinkles", "prevention", "improvement" and "food" in the present invention are as described above.

In an embodiment of the present invention, it was confirmed that the Sukhumi extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increase effect and moisture retention ability were very excellent (Table 7) , since it was confirmed that the skin elasticity improvement effect is very excellent (Table 8), it can be effectively used to improve skin elasticity, prevent aging, and prevent or improve skin wrinkles.

The food composition of the present invention may include various weight % as long as it can exhibit skin elasticity improvement, aging prevention, skin wrinkle prevention or improvement effect, but specifically, sukhumid extract or a fraction thereof from 0.00001 to 10% by weight relative to the total weight of the food composition can be included as

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating skin wrinkles, comprising the composition for promoting hyaluronic acid production as an active ingredient.

The terms "hyaluronic acid", "skin wrinkles" and "prevention" of the present invention are as described above.

As used herein, the term “treatment” refers to any action that improves or benefits skin wrinkles that have already occurred by administration of the composition.

In an embodiment of the present invention, it was confirmed that the Sukhumi extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the skin moisture content increase effect and moisture retention ability were very excellent (Table 7) , since it was confirmed that the skin elasticity improvement effect is very excellent (Table 8), it can be effectively used for skin elasticity improvement, aging prevention, skin wrinkle prevention or treatment.

The pharmaceutical composition of the present invention may include various weight % as long as it can exhibit a skin wrinkle prevention or treatment effect, but specifically, sukhumi hwang extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the pharmaceutical composition. .

Another aspect of the present invention provides a cosmetic composition for moisturizing the skin comprising the composition for promoting hyaluronic acid production as an active ingredient.

The formulation and carrier of the hyaluronic acid, cosmetic composition of the present invention are as described above.

As used herein, the term “moisturizing” refers to maintaining homeostasis of a living body by appropriately controlling moisture loss (water evaporation), etc. When hyaluronic acid in the skin decreases, moisture is lost, and when hyaluronic acid increases, moisture increases. can do.

In an embodiment of the present invention, it was confirmed that the Sukhumihuang extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing the amount of skin moisture and the water retention ability were very excellent (Table 7) , can be effectively used to moisturize the skin.

The cosmetic composition of the present invention may include various weight % as long as it can exhibit a skin moisturizing effect, but specifically, the Sukhumi hwang extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the cosmetic composition.

Another aspect of the present invention provides a food composition for moisturizing the skin comprising the composition for promoting hyaluronic acid production as an active ingredient.

"Hyaluronic acid", "moisturizing" and "food" of the present invention are as described above.

In an embodiment of the present invention, it was confirmed that the Sukhumihuang extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing the amount of skin moisture and the water retention ability were very excellent (Table 7) , can be effectively used to moisturize the skin.

The food composition of the present invention may contain various weight % as long as it can exhibit a skin moisturizing effect, but specifically, the Sukhumihuang extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight based on the total weight of the food composition.

Another aspect of the present invention provides a quasi-drug composition for skin moisturizing comprising the composition for promoting hyaluronic acid production as an active ingredient.

"Hyaluronic acid", "moisturizing" and "quasi-drug" of the present invention are the same as described above.

In an embodiment of the present invention, it was confirmed that the Sukhumihuang extract can increase the concentration of hyaluronic acid in human-derived keratinocytes (Table 6), and it was confirmed that the effect of increasing the amount of skin moisture and the water retention ability were very excellent (Table 7) , can be effectively used to moisturize the skin.

The quasi-drug composition of the present invention may contain various weight % as long as it can exhibit a skin moisturizing effect, but specifically, sukhumihuang extract or a fraction thereof may be included in an amount of 0.00001 to 10% by weight relative to the total weight of the quasi-drug composition.

Sukhumi extract or a fraction thereof effectively promotes hyaluronic acid production and expression of hyaluronic acid synthase, so it can be usefully used to protect joints, prevent or treat arthritis, improve skin elasticity, prevent aging, prevent or treat skin wrinkles, and moisturize the skin have.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

production example 1. Preparation of Sulfuric Acid Extract

The Sukhumihuang extract used in the following Examples was an ethanol extract, which was purchased from OBM Lab Co., Ltd. and used.

Example 1. Cytotoxicity evaluation of Sukhumi extract

Cell Counting Kit-8 (Dojindo Lab, USA) for cell division changes in Human Fibroblast-like Synoviocytes (HFLS-OA, Cell Applications) collected from patients with degenerative arthritis for cytotoxicity evaluation of S. was used for measurement.

First, a uniform number of cells were laid out in a 96 well plate, stabilized, and then treated with Sukhumus extract at 5 or 10 ppm and further cultured for 24 hours. After treatment with CCK-8, absorbance was measured at 450 nm after 2 hours. The relative cell viability of the cells treated with the Sukhumus extract compared to the untreated group (control group) was analyzed as follows, and the results are shown in Table 1 below.

sample unprocessed glucosamine
1000 ppm Sulfuric Acid Extract
5 ppm Sulfuric Acid Extract
10 ppm Survival rate (%) 100 97 102 105

* number of repetitions = 3

* Relative cell viability = (extract treated group)/(untreated group) X 100

As shown in Table 1 above, it was found that the number of cells increased by the treatment with the Sukhumus extract rather than the untreated group and the glucosamine-treated group. This suggests that the sukhumia extract does not cause cytotoxicity.

Example 2. Patients with degenerative arthritis in synovial cells Increase of hyaluronic acid concentration by S.

Human fibroblast-like synovial cells derived from degenerative arthritis patients were cultured in human periosteal cell growth medium (Synoviocyte Growth Medium, Cell Applications), and seeded at 2.0x10 ³ cells per well in a 96-well plate. After culturing for 3 days, 200 μl of human periosteal cell growth medium was exchanged, and Sukhumi hwang extract was added at different concentrations (5 and 10 ppm). The same amount of ethanol was treated as a negative control, and 1000 ppm of glucosamine hydrochloride (Sigma-aldrich), which is known to promote hyaluronic acid production, was treated as a positive control. After culturing for 24 hours, the cell culture medium was recovered and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), and it is shown in Table 2.

sample unprocessed
(Negative control group) glucosamine
1000 ppm Sulfuric Acid Extract
5 ppm Sulfuric Acid Extract
10 ppm hyaluronic acid
Relative concentration (%) 100 146 136 139

* number of reps = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with S.

As a result, Sukhumi Hwang extract increased the production of hyaluronic acid in synovial cells of degenerative arthritis patients in a concentration-dependent manner. In addition, the Sukhumi extract exhibited a similar hyaluronic acid concentration in an amount 100 to 200 times less than that of the positive control.

Through this, it was confirmed that the extract of Sukhumia extract is very excellent in promoting the production of hyaluronic acid in the synovial cells of degenerative arthritis patients, so it can be usefully used for joint protection or prevention or treatment of arthritis.

Example 3. Patients with degenerative arthritis in synovial cells Hyaluronic acid synthetase (hyaluronic acid synthase HAS ) increased expression of genes

Human fibroblast-like synovial cells derived from degenerative arthritis patients were dispensed with human periosteal cell proliferation medium in a 6-well plate and cultured for 3 days. Cells were recovered, total RNA was extracted with RNeasy mini kit (Qiagen), quantified, and 1 μg of RNA was reverse transcribed using GeneAmp ^® RNA PCR kit (Applied Biosystems). Reverse transcription reaction was performed using Mycycler ^® PCR instrument (Biorad).

Then, quantitative real-time PCR was performed using 2 μl of the reverse transcription solution and Taqman ^® probe (Invitrogen, USA, Hs00193435_m1, Hs00193436_m1) of HAS-2, HAS-3, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase). Real time PCR was performed using CFX96 Touch ^TM Real-Time PCR Detection System (Biorad). Experimental results obtained through real-time PCR were calculated and shown by the method described in Livak KJ et al. (Methods, 2001, 25, 402-408) based on GAPDH, a housekeeping gene.

In addition, the mRNA expression level of the experimental group treated with the Sukhumi extract treated with the mRNA expression level of the negative control group as the standard (100%) was quantified and shown in Table 3.

sample unprocessed
(Negative control group) glucosamine
1000 ppm Sulfuric Acid Extract
5 ppm Sulfuric Acid Extract
10 ppm HAS-2
Expression (%) 100 113 105 138 HAS-3
Expression (%) 100 121 124 145

* number of reps = 4

As a result, the expression of hyaluronic acid synthase, especially HAS-2 and HAS-3, was increased in the synovial cells of patients with degenerative arthritis by treatment with the Sukhumihuang extract. In addition, the Sukhumvitae extract showed a similar HAS-2 expression level as the positive control group even at 200 times less than the glucosamine-treated group, which is a positive control group, and showed a higher HAS-3 expression level.

Through this, it was confirmed that the Sukhumihuang extract is very effective in increasing the expression of hyaluronic acid synthetase in the synovial cells of degenerative arthritis patients, and thus can be usefully used for joint protection or arthritis prevention or treatment.

Example 4. Normal in synovial cells Increase of hyaluronic acid concentration by S.

Human fibroblast-like synovial cells were cultured in human periosteal cell growth medium (Synoviocyte Growth Medium, Cell Applications), and seeded in a 96-well plate at 2.0x10 ³ cells per well. After culturing for 3 days, 200 μl of human periosteal cell growth medium was exchanged, and 10 ppm of Sukhumihuang extract was added. The same amount of solvent was treated as a negative control, and 1000 ppm of glucosamine hydrochloride was treated as a positive control. After culturing for 24 hours, the cell culture medium was recovered, and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), and it is shown in Table 4.

sample unprocessed glucosamine
1000 ppm Sulfuric Acid Extract
10 ppm HA Relative Concentration
(%) 100 146 132

* number of reps = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with S.

As a result, Sukhumihuang extract increased the production of hyaluronic acid in normal synovial cells. In addition, the Sukhumi hwang extract showed a similar hyaluronic acid concentration to the positive control, even in an amount 100 times less than that of the positive control.

Through this, it was confirmed that the extract of Sukhumus extract is very excellent in promoting the production of hyaluronic acid in normal synovial cells, and thus it can be usefully used for joint protection or prevention or treatment of arthritis.

Example 5. rat in the joint cavity Increase of hyaluronic acid concentration by S.

After purchasing a male Sprague-Dawley Rat (Samtaco, Korea) weighing around 200g, acclimatized in a constant temperature and humidity breeding system (Daejong Machinery Co., Korea) adjusted to a temperature of 23±1℃ and a humidity of 55±5% for 1 week. used in the experiment. To the sample treatment group (n=8), the extract was diluted in physiological saline and 100 or 500 mg per 1 kg of rat body weight, respectively, was orally administered once a day for 15 days. In the case of the control group (n=8), only the solvent was administered in the same way. did Thereafter, the femur and tibia were excised together to measure the hyaluronic acid concentration, and then 0.5 ml of physiological saline was injected into the left knee joint and washed, and 100 μl or more of the washed synovial fluid was collected and stored frozen at -70° C. until the test. The hyaluronic acid concentration in the synovial fluid was measured using a Hyaluronan ELISA kit (R&D Systems) and shown in Table 5.

control sample treatment group Serving size per serving - 100mg/kg 500mg/kg Relative concentration of hyaluronic acid (%) 100 112 135

* Numericalize the hyaluronic acid concentration of the sample treatment group based on the hyaluronic acid concentration of the control group (100%)

As a result, Sukhumihuang extract increased the production of hyaluronic acid in the joint cavity of normal rats.

Through this, the extract of Sulfuric Acid is in As it was confirmed that the effect of promoting hyaluronic acid production in the rat joint cavity in vivo was very excellent, it could be usefully used for joint protection or prevention or treatment of arthritis.

Example 6. HaCaT Increased hyaluronic acid concentration in cells by S. Sukhumus extract

Human-derived keratinocytes, HaCaT cells, were prepared at 2x10 ⁴ cells/ml in DMEM medium containing 10% FBS, and 1 ml each was dispensed in a 24-well plate. After culturing for 24 hours, 1 ml of DMEM medium containing no bovine serum was exchanged, and 30 ppm of Sukhumihuang extract was added. The same amount of ethanol was treated as a negative control, and 100 ppm of glucosamine hydrochloride (Sigma-aldrich), which is known to promote hyaluronic acid production, was treated as a positive control. After 48 hours of incubation, the cell culture medium was recovered and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems), and it is shown in Table 6.

sample unprocessed
(negative control) glucosamine
100 ppm Sulfuric Acid Extract
30 ppm hyaluronic acid
Relative concentration (%) 100 199 221

* number of reps = 4

* Based on the hyaluronic acid concentration of the negative control group (100%), the hyaluronic acid concentration of the experimental group treated with S.

As a result, Sukhumihuang extract increased the production of hyaluronic acid in human-derived keratinocytes.

Through this, it was confirmed that the extract of Sukhumihuang extract is very effective in promoting the production of hyaluronic acid in keratinocytes derived from the human body, improving skin elasticity, preventing aging, preventing or treating skin wrinkles; And it can be usefully used for moisturizing the skin.

Example 7. Increase in skin moisture content by extract

In order to apply the effect of increasing the skin moisture content of the cosmetic containing the Sukhumus extract to human skin, it was prepared according to the composition of the nutritional cream of Formulation Example 2 below, and 30 healthy adults in a constant temperature and humidity room at 22° C. and 50% relative humidity. A certain amount (2 mg/cm 2 ) was applied inside, and the moisture content of the skin was measured before application, 1 hour after application, and 2 hours after application. In the case of the control group, a nourishing cream containing the same amount of ethanol was prepared and applied in the same way instead of the Sukhumihuang extract, and normal skin without any treatment was used as the untreated group. The water retention capacity was measured by measuring the electrical conductivity of the skin surface using a Corneometer (Courage + Khazaka, GmbH, Germany). The results are shown in Table 7 below.

unit (corneometer units) Formulation Example 2
nourishing cream control
nourishing cream untreated group Skin electrogram before application 55 54 56 1 hour after application 104 81 55 2 hours after application 94 67 52

* n=30

As a result, the nourishing cream containing Sukhumus extract increased the amount of moisture in human skin, and showed excellent moisture retention ability even after 2 hours of application.

Through this, it was confirmed that the extract of Sukhumihuang extract is very excellent in skin moisture content increase effect and moisture retention ability, improving skin elasticity, preventing aging, preventing or treating skin wrinkles; And it can be usefully used for moisturizing the skin.

Example 8. Enhanced skin elasticity by extract

In order to apply the skin elasticity enhancing effect of the cosmetic containing the extract of the present invention to human skin, it was prepared according to the composition of the nourishing cream of Formulation Example 2 below, and healthy adults in a constant temperature and humidity room at 22° C. and 50% relative humidity. After applying it to the neck of 30 people for 4 weeks, the elasticity before and after the chart was measured to compare and evaluate the skin elasticity improvement effect.

In the left part of the subject's neck, a nourishing cream containing sukhumia extract was used, and on the right part, a nourishing cream containing the same amount of ethanol instead of sukhumang extract was used twice a day, morning and evening, for 4 weeks after cleansing. Thereafter, the degree of improvement in elasticity in each area was measured using a skin elasticity measuring instrument (Cutometer, C+K, Germany). For the degree of improvement, Ur/Uf was used as a parameter, and the results are shown in Table 8.

Formulation Example 2
nourishing cream control
nourishing cream Elasticity improvement rate (%) 27.89 3.75

* n=30

As a result, the nourishing cream containing Sukhumi extract improved the elasticity of human skin, and did not show any side effects in the skin.

Through this, it was confirmed that the extract of Sukhumus extract is very effective in improving skin elasticity, and thus it can be usefully used for improving skin elasticity, preventing aging, and preventing or treating skin wrinkles.

Based on the above effect test results, various formulation examples of the composition according to the present invention will be described in detail. However, these formulation examples are only examples of the composition of the present invention, it will be apparent to those of ordinary skill in the art that the scope of the composition of the present invention is not limited only to these formulations.

Formulation example One. of health drinks Produce

Sulfuric Acid Extract 0.1 mg

citric acid 1000 mg

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, after stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L container, sealed and sterilized, then refrigerated and then stored in the present invention was used to prepare a health drink composition of

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demand country, and use purpose.

Formulation example 2. Manufacture of nourishing cream

As shown in the following composition, a nutritious cream containing sukhumihuang extract as an active ingredient was prepared according to a conventional method.

1.0% by weight of Sulfuricaceae Extract

Beta-1,3-glucan 5.0 wt%

Beeswax 10.0 wt%

Polysorbate 60 1.5 wt%

Sebum 60 hydrogenated castor oil 2.0 wt%

0.5 wt% of sorbitan sesquioleate

Liquid paraffin 10.0 wt%

5.0 wt% of squalane

5.0 wt% caprylic/capric triglyceride

glycerin 5.0 wt%

Butylene glycol 3.0 wt%

Propylene glycol 3.0 wt%

0.2 wt% of triethanolamine

Preservative 0.05 wt%

dye 0.05% by weight

Flavor 0.05 wt%

Purified water to 100% by weight

Formulation example 3: Preparation of drugs for topical administration (gel ointment)

As shown in the composition below, a gel ointment containing the extract of Sukhumus extract as an active ingredient was prepared according to a conventional method.

0.5% by weight of Sulfuricaceae Extract

Beta-1,3-glucan 10.0 wt%

1.5 wt% polyacrylic acid (Carbopol 940)

5.0% by weight of isopropanol

Hexylene glycol 25.0 wt%

1.7% by weight of triethanolamine

deionized water to 100% by weight

Formulation example 4: Preparation of granules

Sulfuric Acid Extract 2 mg

Soybean Extract 50 mg

glucose 200 mg

Starch 600 mg

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60° C. to form granules, and then filled in a bag.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.

Claims (6)

delete A food composition for joint protection comprising Rehmanniae Radix Preparata extract or a fraction thereof as an active ingredient, wherein the joint protection is by promoting hyaluronic acid production.
A quasi-drug composition for joint protection comprising an extract of Rehmanniae Radix Preparata or a fraction thereof as an active ingredient, wherein the joint protection is by promoting the production of hyaluronic acid, a quasi-drug composition for joint protection.
delete The food composition according to claim 2, wherein the food composition comprises 0.00001 to 10% by weight of the Sukhumihuang extract or a fraction thereof based on the total weight of the composition.
The quasi-drug composition according to claim 3, wherein the quasi-drug composition comprises 0.00001 to 10% by weight of the Sukhumus extract or a fraction thereof based on the total weight of the composition.