KR101452582B1 - Compositions for improving skin wrinkle or enhancing skin elasticity comprising tetrahydropalmatine as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles or skin elasticity, and more particularly, to a cosmetic composition for improving skin wrinkles containing Tetrahydropalmatine as an active ingredient. The tetrahydro-palmatine of the present invention promotes the collagen synthesis, thereby enhancing the elasticity of the skin and improving the skin wrinkle.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving skin wrinkles or skin elasticity comprising tetrahydro-palmatine as an active ingredient,

The present invention relates to a composition for improving skin wrinkles or improving skin elasticity, and more particularly, to a composition for improving skin wrinkles or improving skin elasticity comprising Tetrahydropalmatine as an active ingredient, a food composition, and a pharmaceutical composition .

Because the skin has many opportunities to interact with various external stimuli, it is more prone to wrinkles than other organs. Among them, facial skin is directly exposed to sunlight, dry outside air and pollutants, so that aging phenomenon such as wrinkles occurs more early than other skin.

The most characteristic change due to aging of the skin tissue is a change in the skin matrix, which causes aging of the human skin fibroblast in the dermis, resulting in a decrease in the ability to produce fibrous and matrix. The amount of the substrate is generally decreased, the thickness of the skin is thinned, and the elasticity of the skin is lowered to form the wrinkles. That is, as aging progresses, the skin becomes weaker in elasticity, disorder of blood circulation, weakening of skin barrier, and the like.

Collagen, a major component of the extracellular matrix, is a major substrate protein in the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). In most other structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis.

The functions of collagen present in the skin are known as mechanical rigidity of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or healing) (van der Rest et al., 1990). Such collagen is reduced by aging and UV irradiation, which is known to be closely related to skin wrinkling and skin elasticity (Arthur K. Balin et al., "Aging and the skin ", 1989). In recent years, as a result of extensive research on skin aging, important functions of collagen in the skin have been revealed.

Conventionally, there have been products in which collagen is formulated into cosmetics by using the skin moisturizing effect of collagen. However, since these cosmetics are coated with collagen on the surface of the skin, the moisturizing action due to the transdermal absorption of the polymer collagen can not be expected, It can not be said that it is functional improvement.

As a collagen synthesis promoting substance, retinoic acid, TGF (transforming growth factor carcinogenesis factor; Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974) -231370), betulinic acid (JP8-208424), chlorella extract (JP9-40523, JP10-36283, fibroblast proliferation promoting action) are known, but when applied to the skin, there are safety problems such as stimulation and redness The use amount is limited or the effect is insignificant, so that the skin collagen synthesis is substantially promoted and the skin function improving effect can not be expected. Therefore, it is urgently required to develop a new collagen synthesis promoter which is safe for living body and is more effective than the existing collagen synthesis performance promoting substance.

Accordingly, the present inventors have made intensive efforts to develop a substance having an excellent collagen synthesis promoting effect, and as a result, it has been found that tetrahydropalmatine has a very strong promoting effect on collagen synthesis, and it is used for improving skin wrinkles and skin elasticity The present invention has been completed.

An object of the present invention is to provide a cosmetic composition, a food composition or a pharmaceutical composition for improving skin wrinkles or skin elasticity enhancement comprising Tetrahydropalmatine as an active ingredient.

Another object of the present invention is to provide a composition for promoting collagen synthesis comprising Tetrahydropalmatine as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles or improving skin elasticity comprising Tetrahydropalmatine as an active ingredient.

The Tetrahydropalmatine has a chemical structure of the following formula (1).

"Tetrahydropalmatine" of the present invention can be obtained by conventional chemical synthesis, separated from natural products, or sold and used . The natural ground is part of banggigwa plants Tnbraurearecisaporrc, yeonam (Stephaniasinica Diels ) Roots, Anasms and plants Corydalis yanhusuo WT Wang) of the stem, some Corydalis (Corydalis decumbens (Thunb.) Per . S. ) stems, and the like, but are not limited thereto.

As the active ingredient of the pharmaceutical composition of the present invention, the tetrahydropalmatine can be used in the form of a pharmaceutically acceptable salt thereof, wherein the salt includes an acid formed by a pharmaceutically acceptable free acid Additional salts are useful. Acid addition salts can be prepared in a conventional manner, for example, by dissolving the compound in an excess amount of an aqueous acid solution and precipitating the salt with a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile. The same molar amount of the compound and the acid or alcohol (e.g., glycol monomethyl ether) in water may be heated and then the mixture may be evaporated to dryness, or the precipitated salt may be subjected to suction filtration. As the free acid, organic acids and inorganic acids can be used. As the inorganic acids, hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, tartaric acid and the like can be used. Examples of the organic acids include methanesulfonic acid, p- toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, citric acid, lactic acid, glycollic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid and hydroiodic acid may be used. It is not limited.

In addition, bases can be used to make pharmaceutically acceptable metal salts. The alkali metal or alkaline earth metal salt is obtained, for example, by dissolving the compound in an excess of an alkali metal hydroxide or an alkaline earth metal hydroxide solution, filtering the non-soluble compound salt, and evaporating and drying the filtrate. At this time, as the metal salt, it is preferable to produce sodium, potassium or calcium salt particularly, but not limited thereto. The corresponding silver salt can also be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (e.g., silver nitrate).

The pharmaceutically acceptable salts of Tetrahydropalmatine substantially include salts of acidic or basic groups that may be present in the compounds of Tetrahydropalmatine, unless otherwise indicated. For example, pharmaceutically acceptable salts may include sodium, calcium and potassium salts of hydroxy groups, and other pharmaceutically acceptable salts of amino groups include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, (Mesylate), p-toluenesulfonate (tosylate) salts, and the like, which are known in the art and which can be prepared by methods known to those skilled in the art, such as hydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate .

In the present invention, Tetrahydropalmatine also includes a derivative thereof within the range of the same efficacy. The Tetrahydropalmatine may also include solvates, hydrates and stereoisomers which exhibit the same potency as can be prepared therefrom.

The cosmetic composition comprising Tetrahydropalmatine of the present invention promotes the synthesis of collagen, thereby exhibiting the effect of improving skin wrinkles or enhancing skin elasticity. In one embodiment of the present invention, the composition of the present invention is administered to a culture medium of human-derived fibroblasts, and the biosynthesized collagen is measured. As a result, the tetrahydropalmatine increases collagen synthesis in a concentration- Derived fibroblasts were found to have excellent collagen synthesis ability (Example 1, Table 1). In an embodiment of the present invention, As a result of measuring the wrinkle-reducing effect after treating the composition of the present invention, it was confirmed that the area treated with Tetrahydropalmatine had a wrinkle-reducing effect superior to that of the control (no treatment) 2, Table 2)

The term "skin wrinkle " in the present invention means a skin caused by skin decline, which may be caused by a cause of a gene, collagen and elastin present in skin dermis, external environment, and the like.

In the present invention, "improvement of skin wrinkles" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

In the present invention, the term "skin elasticity" is expressed by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber, When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, and elastin is a protein that is found only in elastic fibers such as desmosine and isodesmosine derived from lysine, It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, the term "skin elasticity enhancement" means that elastic fibers composed of elastin are present together with collagen fibers called collagen, and elastic elasticity is maintained in a state in which elastin and collagen are sufficiently present.

The cosmetic composition for improving skin wrinkles of the present invention may contain 0.000001% by weight to 10% by weight, more preferably 0.001% by weight to 10% by weight, based on the total weight of the composition, of tetrahydropalmatine, 0.1% by weight to 10% by weight. If the content of the tetrahydropalmatine is less than 0.000001% by weight, no remarkable effect can be expected. If the content exceeds 10% by weight, the effect is not increased as compared with the content.

The cosmetic composition for improving skin wrinkles according to the present invention can be used as a cosmetic composition for improving wrinkles of skin, which can be used as a solution, an external ointment, a cream, a foam, a nutrition lotion, a softening water, a pack, May be formulated into a formulation selected from the group consisting of an oil, a suspension, an emulsion, a paste, a gel, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powdered foundation, an emulsion foundation, a wax foundation, , But is not limited thereto.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations. When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, .

The composition of the present invention may further comprise at least one substance having a function of improving skin wrinkles or improving skin elasticity.

In another aspect, the present invention provides a method for improving skin wrinkles or a method for enhancing skin elasticity, comprising the step of applying the tetrahedropalmatine to the skin of an individual.

The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

In another aspect, the present invention provides a food composition for improving skin wrinkles or improving elasticity comprising the above-mentioned tetrahydropalmatine as an active ingredient.

Foods containing the compounds of the present invention include, for example, various foods, beverages, gums, tea, vitamin complex, and the like, but are not limited thereto.

The food for improving skin wrinkles of the present invention includes forms such as tablets, capsules, pills, and liquids.

The beverage composition for improving skin wrinkles of the present invention is not particularly limited to other ingredients except for containing the above-mentioned compound as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the compound of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the compounds of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

In another aspect, the present invention provides a pharmaceutical composition for improving skin wrinkles or skin elasticity containing the above-mentioned tetrahydropalmatine as an active ingredient.

The compositions comprising the compounds of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The composition containing the compound of the present invention may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations, suppositories, Examples of carriers, excipients and diluents that can be contained in the composition containing the compound include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, Calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions and syrups. In addition to water and liquid paraffin, which are commonly used diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the compound of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the compound of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 1 to 20 mg / kg per day. The administration may be carried out once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

In another aspect, the present invention provides a composition for accelerating collagen synthesis comprising the tetrahydropalmatine as an active ingredient.

Tetrahydropalmatine of the present invention is used as a collagen synthesis promoter which is safe to the living body and promotes collagen synthesis of fibroblasts of the skin, thereby improving skin elasticity and improving skin wrinkles.

The content of tetrahydropalmatine as an active ingredient of the collagen synthesis promoter may be 0.00001 to 99.9% by weight or may be included in the total amount, and is not particularly limited as long as it is in an effective content range.

The cosmetic composition for improving skin wrinkles according to the present invention has a strong collagen synthesis promoting activity and contains Tetrahydropalmatine which is stable in manufacturing and storage of cosmetics as an active ingredient so that it has no toxicity and stability and is easy to be formulated And is excellent in improving the wrinkles of the skin, so that it can be usefully used in the cosmetics industry and the pharmaceutical industry.

Hereinafter, the present invention will be described in more detail by way of examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not construed as being limited by these embodiments.

Example  One: Tetrahydro-palmatine ( Tetrahydropalmatine ) Of collagen synthesis Ability

The effect of wrinkle improvement or skin elasticity improvement can usually be measured as collagen biosynthesis performance, collagenase degradation inhibition ability and human clinical effect. Tetrahydropalmatine was added to the culture medium of human-derived fibroblasts to examine the promoting effect of collagen synthesis at the cellular level. The biocompatible collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

Human normal fibroblasts were cultured in DMEM medium at 37 ° C for 24 hours in a 5% CO ₂ incubator. Tetrahydropalmatine (10 ㎍ / ml, 20 ㎍ / ml) and vitamin C (Vitamin C) were added to the human fibroblast culture medium, Lt; / RTI > After 1 day, the amount of collagen biosynthesis was determined by the PICP EIA kit at 450 nm using a spectrophotometer. The collagen biosynthesis performance was measured by the relative increase in the relative performance as compared with the control (untreated) and is shown in Table 1.

Additive sample density Increase in collagen synthesis (%) compared to control Tetrahydropalmatine 10 μg / ml 16% Tetrahydropalmatine 20 / / ml 39% Vitamin C 52.8 g / ml 30.6%

                                                            * Number of repetitions = 6

As shown in Table 1, Tetrahydropalmatine according to the present invention showed an increase in collagen synthesis in a concentration-dependent manner, indicating excellent collagen synthesis ability against human-derived fibroblasts.

Example  2: Tetrahydro-palmatine ( Tetrahydropalmatine ) Wrinkle-improving effect

The effect of wrinkle improvement was measured using the experimental group treated with tetrahydro - palmatine and the untreated control group. Wrinkle-improving effect The animal test was performed using a hairless mouse that was 6 weeks old. The sample was applied with tetrahydro-palmatine in a concentration of 0.2% in 1,3-butylene glycol. Hairless mice were irradiated with 2 MED (minimal crythema dosis) using a solar simulator for 3 days and 10 weeks for 1 week to form wrinkles. Then, 1,3-butylene glycol Butylene glycol) 1 to the control group and 0.2% of tetrahydro-arm Martin handle twice daily 0.1 ml / cm ² (tetrahydro-arm by 0.2 mg / cm ²⁾ Martin 6 weeks treatment improvement of the qualitative determination targeting a group Respectively.

The degree of wrinkle improvement was judged by visual inspection through visual observation and photographing of the sample treatment site, and then the sample treatment group and the control group were compared with each other before the sample treatment to determine no improvement, slight improvement, Respectively.

sample No improvement Slight improvement A significant improvement Control group 10 0 0 Tetrahydropalmatine One 5 4

The number of individuals in each group = 10 As shown in Table 2 above, it was confirmed that the site treated with tetrahydro-palmatine had an excellent wrinkle-reducing effect as compared with the control.

Manufacturing example  1 and Comparative Example  1: tetrahydropalmatine ( Tetrahydropalmatine ) Containing ointment for external skin

Examples of external skin ointment formulations containing Tetrahydropalmatine are shown in Table 3.

Composition Production Example 1 (% by weight) Comparative Example 1 (% by weight) - tetrahydropalmatine
- diethyl sebacate
- Writers
- polyoxyethylene oleyl ether
- Phosphate
- Sodium benzoate
- Vaseline 0.5
8
5
6

Suitable amount
to 100 -
8
5
6

Suitable amount
to 100

Manufacturing example  2 and Comparative Example  2: Tetrahydro-palmatine ( Tetrahydropalmatine ) ≪ / RTI & Cosmetics  Cream prescription

Examples of formulations of cream in cosmetics containing Tetrahydropalmatine are shown in Table 4.

Composition Production Example 2 (% by weight) Comparative Example 2 (% by weight) - tetrahydropalmatine
- Stearic acid
- Cetanol
- potassium hydroxide
- glycerin
- propylene glycol
- Preservative
- incense
- Purified water 0.2
15.0
1.0
0.7
5.0
3.0
Suitable amount
Suitable amount
to 100 -
15.0
1.0
0.7
5.0
3.0
Suitable amount
Suitable amount
to 100

Manufacturing example  3 and Comparative Example  3: Tetrahydro-palmatine ( Tetrahydropalmatine ) ≪ / RTI & Cosmetics  Prescription of flexible lotion

Examples of the prescription of the soft lotion in cosmetics containing Tetrahydropalmatine are shown in Table 5.

Composition Production Example 3 (% by weight) Comparative Example 3 (% by weight) - tetrahydropalmatine
- ethanol
- Polyoxyethylene polylauric acid
- sorbitan
- Paraoxybenzoic acid methyl
- glycerin
- 1,3-butylene glycol
- incense
- Pigment
- Purified water 0.1
10.0
1.0

0.2
5.0
6.0
Suitable amount
Suitable amount
to 100 -
10.0
1.0

0.2
5.0
6.0
Suitable amount
Suitable amount
to 100

Manufacturing example  4 and Comparative Example  4: Tetrahydro-palmatine ( Tetrahydropalmatine ) ≪ / RTI & Cosmetics  Prescription of middle-class lotion

Examples of prescription of the nutritional lotion among cosmetics containing Tetrahydropalmatine are shown in Table 6.

Composition Production Example 4 (% by weight) Comparative Example 4 (% by weight) - tetrahydropalmatine
- Vaseline
- sesquioleic acid sorbitan
- polyoxyethylene oleyl ethyl
- Paraoxybenzoic acid methyl
- propylene glycol
- ethanol
-Carboxyvinyl polymer
- potassium hydroxide
- Pigment
- incense
- Purified water 0.05
2.0
0.8
1.2
Suitable amount
5.0
3.2
18.0
0.1
Suitable amount
Suitable amount
to 100 -
2.0
0.8
1.2
Suitable amount
5.0
3.2
18.0
0.1
Suitable amount
Suitable amount
to 100

Manufacturing example  5 and Comparative Example  5: Tetrahydro-palmatine ( Tetrahydropalmatine ) ≪ / RTI & Cosmetics  Prescription Pack

The prescription examples of packs in cosmetics containing Tetrahydropalmatine are shown in Table 7.

Composition Production Example 5 (% by weight) Comparative Example 5 (% by weight) - tetrahydropalmatine
- glycerin
- propylene glycol
- Polyvinyl alcohol
- ethanol
- polyoxyethylene oleyl ether
- Paraoxybenzoic acid methyl
- Pigment
- incense
- Purified water 0.05
5.0
4.0
15.0
8.0
1.0
0.2
Suitable amount
Suitable amount
to 100 -
5.0
4.0
15.0
8.0
1.0
0.2
Suitable amount
Suitable amount
to 100

Production Example 6 and Comparative Example 6: Essence prescription in cosmetics containing tetrahydropalmatine

Examples of the prescription of the essence in cosmetics containing Tetrahydropalmatine are shown in Table 8.

Composition Production Example 6 (% by weight) Comparative Example 6 (% by weight) - tetrahydropalmatine
- propylene glycol
- glycerin
- aqueous solution of sodium hyaluronate (1%)
- ethanol
- Polyoxyethylene hardened castor oil
- Paraoxybenzoic acid methyl
- incense
- Purified water 0.2
10.0
10.0
5.0
5.0
1.0
0.1
Suitable amount
to 100 -
10.0
10.0
5.0
5.0
1.0
0.1
Suitable amount
to 100

Example 3: Effect of improving the wrinkles of Essence of Production Example 6 and Comparative Example 6

Thirty female females from 35 to 50 years old were divided into two groups by 15 persons. Group 1 contained the essence of Preparation Example 6 containing 0.2% of tetrahydropalmatine and Group 2 contained the essence of Comparative Example 6 And applied to the face part twice a day for 3 months. After 3 months, the improvement of wrinkles was evaluated by image analysis of questionnaires and wrinkles. The subjects' questionnaires were compared with those before use with respect to improvement of wrinkles and elasticity. No improvement, slight improvement, and considerable improvement were judged in three stages. The results are shown in Table 9.

sample No improvement Slight improvement A significant improvement Comparative Example 6 9 5 One Production Example 6 - 8 7

As shown in Table 9, it was found that the use of Production Example 6 according to the present invention was superior to the Comparative Example in the improvement of wrinkles and elasticity.

Immediately after the experiment, the replica was collected from the same area under the eye. The image was analyzed by image analysis to determine the degree of wrinkles The density of wrinkles was measured by dimensional analysis. The results of the measurement of the wrinkle density by image analysis are shown in Table 10 as averages of the reduction rate relative to the wrinkle density before use.

sample Wrinkle density reduction rate (%) Comparative Example 6 8% Production Example 6 42%

As shown in Table 10, when the preparation example 6 to which Tetrahydropalmatine according to the present invention was added was applied, it was found that the density of wrinkles was greatly reduced before use.

Claims (9)

A cosmetic composition for improving skin wrinkles or skin elasticity comprising Tetrahydropalmatine represented by the following formula (1) as an active ingredient.
[Chemical Formula 1]

The cosmetic composition according to claim 1, wherein the content of Tetrahydropalmatine is 0.000001 to 10% by weight based on the total weight of the composition.
The cosmetic composition according to claim 1, wherein the tetrahydropalmatine promotes collagen synthesis.
The composition of claim 1, wherein the composition is selected from the group consisting of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening longevity pack, a pack, a softener, a latex, a makeup base, an essence, Characterized by having a formulation selected from the group consisting of suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays Composition.
The cosmetic composition of claim 1, further comprising a cosmetically acceptable carrier.
delete delete delete A composition for promoting collagen synthesis, comprising Tetrahydropalmatine represented by the following formula (1) as an active ingredient.
[Chemical Formula 1]