KR20140051763A

KR20140051763A - Cosmetic composition for anti-wrinkle

Info

Publication number: KR20140051763A
Application number: KR1020130070312A
Authority: KR
Inventors: 김선영; 최승만; 김일구
Original assignee: 주식회사 엘지생활건강
Priority date: 2012-10-23
Filing date: 2013-06-19
Publication date: 2014-05-02

Abstract

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly to a cosmetic composition containing two or more kinds selected from the group consisting of type 3 collagen recombinant protein, type 3 collagen derived peptide, and Asian thioside, To prevent or improve the wrinkles of the skin or to improve skin elasticity. The present invention also relates to a food composition for improving wrinkle or skin elasticity, a pharmaceutical composition, and a collagen-containing composition comprising two or more kinds selected from the group consisting of a collagen type III collagen protein, a type III collagen peptide, And to a composition for promoting synthesis. The cosmetic composition according to the present invention contains two or more kinds of compounds in the group consisting of the type III collagen recombinant protein, the type III collagen-derived peptide, and the anticloside, so that the cosmetic composition has superior effects when used alone, It is expected to be widely used as a cosmetic composition, a food composition, a pharmaceutical composition and a composition for promoting collagen synthesis for improving wrinkles or improving skin elasticity.

Description

[0001] Cosmetic composition for anti-wrinkle [

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly to a cosmetic composition containing two or more kinds selected from the group consisting of type 3 collagen recombinant protein, type 3 collagen derived peptide, and Asian thioside, To prevent or improve the wrinkles of the skin or to improve skin elasticity. The present invention also relates to a food composition for improving wrinkle or skin elasticity, a pharmaceutical composition, and a collagen-containing composition comprising two or more kinds selected from the group consisting of a collagen type III collagen protein, a type III collagen peptide, And to a composition for promoting synthesis.

The skin protects the body from the outside, is responsible for maintaining the homeostasis such as body temperature control and sebum secretion. If the functions of the skin are unbalanced due to external chemical and physical stimulation, stress and nutritional status, and aging, the activity of the cells existing in the skin is lowered, and the constituents of the skin can not maintain the original stable structure, .

Components constituting the skin dermis include cells such as fibroblasts and endothelial cells, and substances constituting an extracellular matrix such as collagen, elastin and fibronectin. These extracellular matrix components form and stabilize the structure of the dermis. When they are abnormally produced or degraded, they affect the structure of the entire skin, resulting in wrinkles and skin elasticity.

Collagen is the main component of the extracellular matrix and is the main substrate protein produced by the fibroblasts of the skin and exists in the extracellular matrix. Collagen is an important protein that occupies about 30% of the total weight of bioprotein and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). In most other structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis.

The functions of collagen present in the skin are known as mechanical rigidity of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or healing) (van der Rest et al., 1990). Such collagen is reduced by aging and UV irradiation, which is known to be closely related to skin wrinkling and skin elasticity (Arthur K. Balin et al., "Aging and the skin ", 1989). In recent years, as a result of extensive research on skin aging, important functions of collagen in the skin have been revealed.

In the prior art, there have been products that incorporate collagen into cosmetics from the skin moisturizing effect of collagen, but these cosmetics apply collagen to the skin surface, and since the collagen, which is a polymer, can not be expected to be moisturized by transdermal absorption, Can not be said. Accordingly, in recent years, the function of the active ingredient used for suppressing and improving the production of collagen wrinkles has been approached from the following two viewpoints. The first is to promote collagen production from fibroblasts, and the second is to inhibit collagen degradation by inhibiting collagenase activity.

Research on the materials that can promote collagen biosynthesis has been actively conducted. Materials known to be effective in wrinkle removal include vitamin A and its derivatives (retinoids), vitamin C (vitamin C), transforming growth factor (TGF), animal placenta-derived proteins, betulinic acid Betulinic acid).

Retinoids include retinoic acid, retinal, retinyl acetate, retinyl propionate, retinyl palmitate, retinyl acetate, Vitamin A, or derivatives thereof, such as retinyl linoleate. It is known to exert the effect of wrinkle removal through promoting cellular regeneration of the skin and promoting collagen synthesis. Further, it is reported that it is effective for improving the photoaging. However, retinoids are difficult to apply to products because of safety problems such as irritation and redness when applied to skin and extremely unstable to heat and light. Therefore, it is urgently required to develop a novel composition for external application for skin, which is safer, more stable, and more effective than a conventional skin composition for external use.

Accordingly, the inventor of the present invention solved the above problems and found out that while Asiaticoside was being sold out to find out the composition of a cosmetic composition acting on the type-3 collagen other than the conventional type-I collagen, a more effective wrinkle- Type Ⅲ collagen), and confirmed that the effect is greatly increased due to the use of the collagen-type recombinant protein of type 3 or the collagen-derived peptide of type-3, thereby completing the present invention.

It is an object of the present invention to provide a cosmetic composition containing two or more kinds selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen-derived peptide, and an Asian ticoside.

It is still another object of the present invention to provide a composition for promoting collagen synthesis comprising two or more kinds selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen derived peptide, and an Asian ticoside.

It is another object of the present invention to provide a method for improving or preventing wrinkles of the skin using the composition.

It is still another object of the present invention to provide a qualified composition containing at least two selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen peptide, and an anticholinic acid.

Another object of the present invention is to provide a pharmaceutical composition containing at least two kinds selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen derived peptide, and an Asian ticoside.

In order to achieve the above object, the present invention provides a cosmetic composition comprising at least two selected from the group consisting of a collagen type III collagen protein, a collagen peptide derived from type 3 collagen, and an asialicoside.

As used herein, the term "collagen" is the main component of connective tissue and is a component mainly distributed in bones and skin. It is a light protein that maintains the structure and shape of skin and provides firm strength and elasticity. Exposure to skin aging, ultraviolet light, or inferiority can lead to a decrease in collagen synthesis ability and a rapid decrease in the amount of collagen. When the amount of collagen is reduced, the shape of the muscle can not be maintained and so-called wrinkles are formed.

As used herein, the term " Type III collagen "refers to collagen which is present in a large amount in the fetus among the 27 types of collagen of the collagen, which accounts for about 50% of the collagen of the fetal skin, . However, the rate of growth is gradually decreasing, and it accounts for 1/6 of the type 1 collagen in adults. Thus, a substance that promotes the synthesis of collagen, particularly type 3 collagen, can be used for wrinkle improvement or skin elasticity enhancement.

The term "type 3 collagen recombinant protein" used in the present invention means a type III collagen protein produced in large quantities by transfecting a host cell with a gene of type 3 collagen. Preferably, the type III collagen protein may be a recombinant protein consisting of SEQ ID NO: 1.

As used herein, the term "type 3 collagen peptide" refers to a peptide comprising a part or all of the amino acid sequence of type 3 collagen, and refers to a peptide having an amino acid sequence part And the like. Preferably, it may be a peptide having a high proportion of amino acids of Proline, Hydroxyproline, and Glycine, more preferably a peptide consisting of proline, hydroxyproline, and glycine Peptide. (Proline-hydroxyproline-glycine) n and n is from 30 to 70, preferably from about 30 to about 70, more preferably from about 30 to about 70, Peptide, and may also be a peptide having a molecular weight of 10,000 to 20,000.

As used herein, the term "asiaticoside" refers to a substance extracted from Centella asiatica, which has been used for skin treatment due to burns or wounds. In extracellular matrix, including collagen, matrix of the input signal. It is known that Asian ticoside inhibits the activity of collagenase and increases collagen biosynthesis (Lee J, et al. Int J Cosmet Sci. 2008 Jun; 30 (3): 167-73) . Specifically, it may be a compound having a structure represented by the following formula (1).

As the active ingredient of the composition of the present invention, the above-mentioned asiaticoside can be used in the form of a pharmaceutically acceptable salt thereof. As the salt, an acid addition salt formed by a cosmetically acceptable free acid is useful. Acid addition salts can be prepared in a conventional manner, for example, by dissolving the compound in an excess amount of an aqueous acid solution and precipitating the salt with a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile. The same molar amount of the compound and the acid or alcohol (e.g., glycol monomethyl ether) in water may be heated and then the mixture may be evaporated to dryness, or the precipitated salt may be subjected to suction filtration. As the free acid, organic acids and inorganic acids can be used. As the inorganic acids, hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, tartaric acid and the like can be used. Examples of the organic acids include methanesulfonic acid, p- toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, citric acid, lactic acid, glycollic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid and hydroiodic acid may be used. It is not limited.

In addition, bases can be used to make cosmetically acceptable metal salts. The alkali metal or alkaline earth metal salt is obtained, for example, by dissolving the compound in an excess of an alkali metal hydroxide or an alkaline earth metal hydroxide solution, filtering the non-soluble compound salt, and evaporating and drying the filtrate. The corresponding silver salt can also be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (e.g., silver nitrate).

The cosmetically acceptable salt of the above-mentioned Asian ticoside substantially includes salts of acidic or basic groups which may exist in the compound of the Asian ticoside, unless otherwise indicated. For example, the cosmetically acceptable salts may include sodium, calcium and potassium salts of hydroxy groups, and other pharmaceutically acceptable salts of amino groups include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, (Mesylate), p-toluenesulfonate (tosylate) salts, and the like, which are known in the art and which can be prepared by methods known to those skilled in the art, such as hydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate .

In the present invention, the above-mentioned Asian ticoside also includes a derivative thereof within the range of the same effect. In addition, the above-mentioned Asian ticoside may also contain solvates, hydrates and stereoisomers which exhibit the same efficacy as can be produced therefrom.

In the present invention, the Asian ticoside can be a derivative or an analog obtained through deletion, addition or substitution of a substituent usually carried out in the art. In addition, it is clear from the technical level of the art that any of derivatives or analogues of the Asian ticoside that exhibits the wrinkle-reducing effect is included in the scope of the present invention.

In the present invention, the cosmetic composition may preferably include a peptide derived from type 3 collagen and an Asian ticoside, and most preferably a peptide containing collagen recombinant protein, type 3 collagen derived peptide, and Asian ticoside Lt; / RTI > In addition, the cosmetic composition may exhibit the effect of accelerating the synthesis of collagen type 3, and may exhibit the effect of improving wrinkles or skin elasticity.

In a specific embodiment of the present invention, the effect of improving the wrinkles of a cosmetic composition comprising at least two kinds of group 3 collagen recombinant protein, type 3 collagen-derived peptide and / or Asian thioside was compared. 140 women in their 30s were divided into seven groups of 20 persons each, and the cosmetic composition of various compositions was applied to the eye area designated for 8 weeks, twice a day, morning and evening, respectively. The state of the wrinkles was made by making a simulated silicon plate, and the state of the wrinkles at the designated area was measured with a VISMETOMETER, an image analyzer.

As a result, even though the total ratio of type 3 collagen recombinant protein, type 3 collagen derived peptide, and / or Asian ticoside was kept constant, type 3 collagen recombinant protein, type 3 collagen derived peptide and 2 Synergistic effect showed superior wrinkle improvement effect when using more than one species. Furthermore, when the above-mentioned three components were used in combination, it was confirmed that the effect was 1.8 times to 3 times as much as that in the experimental example using the combination of 9 times and 2 times of the control used alone, (See Example 2 and Table 3). &Lt; tb >< TABLE >

In another specific embodiment of the present invention, the effect of promoting type III collagen synthesis by treating the type III collagen-derived peptide and / or the Asian ticoside was measured. Cells were treated with the addition of type 3 collagen peptide and / or Asian thioside and the amount of type 3 collagen synthesis was measured in the culture supernatant using the Collagen Type III EIA kit. It was confirmed that the amount of collagen biosynthesis was significantly increased due to the synergistic effect due to the simultaneous inclusion of the type III collagen-derived peptide and the Asian ticoside (see Example 3 and Table 4).

In the present invention, the term "wrinkle " means a scarred skin caused by a genetic cause, a decrease in collagen and elastin present in the skin dermis, and an external environment.

In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

In the present invention, the term "skin elasticity" is expressed by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber, When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, and elastin is a protein that is found only in elastic fibers such as desmosine and isodesmosine derived from lysine, It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, the term "skin elasticity enhancement" means that elastic fibers composed of elastin are present together with collagen fibers called collagen, and elastic elasticity is maintained in a state in which elastin and collagen are sufficiently present.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a softening water, a pack, a soft water, an emulsion, a makeup base, But are not limited to, emulsions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays It is not.

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulation. When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, .

The composition of the present invention may further comprise at least one substance having a function of improving skin wrinkles or improving skin elasticity.

In another aspect, the present invention provides a method for preventing or improving wrinkles of the skin or promoting skin elasticity, comprising applying the composition to the skin of an individual.

The subject includes, without limitation, mammals including rats, livestock, humans, and the like.

As used herein, the term "application " refers to any method of contacting a composition according to the present invention to the skin of a subject by any suitable method, thereby aiming to absorb the composition into the skin.

As used herein, the term "prevention" refers to any action that inhibits or delays the occurrence of wrinkles by application of the composition of the present invention. As used herein, the term "improvement" refers to any action that improves or improves wrinkles by application of the composition of the present invention. As used herein, the term " enhancement "refers to any action that increases the elasticity of skin by application of the composition according to the present invention, or slows down the loss of elasticity due to aging.

In another aspect, the present invention provides a composition for accelerating collagen synthesis comprising two or more selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen-derived peptide, and an anticholinergic.

The above-mentioned type 3 collagen recombinant protein, type 3 collagen derived peptide, asiaticoside, collagen and the like are the same as described above.

In another aspect, the present invention provides a food composition for improving wrinkle or skin elasticity comprising at least two kinds selected from the group consisting of a type 3 collagen recombinant protein, a type 3 collagen-derived peptide, and an Asian thioside .

Foods containing the compounds of the present invention include, for example, various foods, beverages, gums, tea, vitamin complex, and the like, but are not limited thereto.

The wrinkle-improving or skin elasticity-enhancing food of the present invention includes forms such as tablets, capsules, pills, and liquids.

The wrinkle-improving or skin elasticity-enhancing beverage composition of the present invention is not particularly limited to the other components other than the above-mentioned compound as the essential ingredient in the indicated ratio, and it is also possible to add various flavors or natural carbohydrates, . Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

In another aspect, the present invention provides a pharmaceutical composition for improving wrinkle or skin elasticity comprising at least two kinds selected from the group consisting of type 3 collagen recombinant protein, type 3 collagen-derived peptide, and Asian thioside .

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The composition of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method Examples of carriers, excipients and diluents that can be contained in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions and syrups. In addition to water and liquid paraffin, which are commonly used diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the pharmaceutical composition of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 1 to 20 mg / kg per day. The administration may be carried out once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The cosmetic composition according to the present invention contains two or more kinds of compounds in the group consisting of the type III collagen recombinant protein, the type III collagen-derived peptide, and the anticloside, so that the cosmetic composition has superior effects when used alone, It is expected to be widely used as a cosmetic composition, a food composition, a pharmaceutical composition and a composition for promoting collagen synthesis for improving wrinkles or improving skin elasticity.

FIG. 1 is a view showing a tripeptide which is a repeating sequence constituting a peptide derived from type 3 collagen in the present invention. FIG.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not construed as being limited by these embodiments.

Example 1. Type 3 collagen recombinant protein, derived from type 3 collagen Peptides Or < RTI ID = 0.0 > Asian < Cosmetics Composition manufacturing

In the present invention, in order to compare the wrinkle-reducing effect of the cosmetic composition comprising two or more kinds of the group consisting of the collagen type III collagen protein, the type III collagen-derived peptide and the Asian thioside, the control group 1 to the control group 4 and the test group 1 to the test group 4 was prepared. The composition ratios of the control groups 1 to 4 and the experimental groups 1 to 4 are shown in Tables 1 and 2 below.

In the following table, the type III collagen-derived peptides were analyzed by analyzing the amino acid sequence of the type-III collagen, and the proline-hydroxyproline-glycine tripeptide binding to glycine, hydroxyproline and proline amino acid As a result of the molecular weight analysis, 10,000 peptides were used.

Composition ratio of cosmetic compositions of Control 1 to Control 4 Raw material name Control 1
(wt%) Control group 2
(wt%) Control group 3
(wt%) Control group 4
(wt%) Caprylic /
Capric triglyceride 5.0 5.0 5.0 5.0 Triethanolhexanone 0.3 0.3 0.3 0.3 Sorbitan stearate 5.0 5.0 5.0 5.0 FAGE-40 stearate 0.02 0.02 0.02 0.02 Cetearyl olivate 0.03 0.03 0.03 0.03 Sorbitan olivate 1.0 1.0 1.0 1.0 Purified water 88.65 88.35 88.35 88.35 Type 3 collagen recombinant protein 0 0.3 0 0 Asian Ticoside 0 0 0.3 0 Type 3 collagen-derived peptide 0 0 0 0.3

Composition ratio of cosmetic composition of Experiment group 1 to Experiment group 4 Raw material name Experiment 1
(wt%) Experiment 2
(wt%) Experiment group 3
(wt%) Experiment group 4
(wt%) Caprylic /
Capric triglyceride 5.0 5.0 5.0 5.0 Triethanolhexanone 0.3 0.3 0.3 0.3 Sorbitan stearate 5.0 5.0 5.0 5.0 FAGE-40 stearate 0.02 0.02 0.02 0.02 Cetearyl olivate 0.03 0.03 0.03 0.03 Sorbitan olivate 1.0 1.0 1.0 1.0 Purified water 88.35 88.35 88.35 88.35 Type 3 collagen recombinant protein 0.15 0.15 0 0.1 Asian Ticoside 0 0.15 0.15 0.1 Type 3 collagen-derived peptide 0.15 0 0.15 0.1

The cosmetic compositions of Control 1 to Control 4 and Experiment 1 to Experiment 4 having the composition ratios of Table 1 and Table 2 were prepared by 1) uniformly mixing the aqueous ingredients at room temperature and heating to 70 to 75 ° C, 2) To 80 ° C, uniformly dissolved and mixed, and 3) the above-mentioned 1) was added under stirring with 2) to emulsify uniformly.

Example 2. Cosmetics Measurement of wrinkle improvement effect of composition

To investigate the effect of the cosmetic compositions of the present invention on the control group 1 to the control group 1 and the control group 4 and the experimental group 1 to the experimental group 4, 140 women in their 30s were divided into 7 groups each consisting of 20 individuals. To the control group 4 and the experimental group 1 to the experimental group 4, respectively. The state of the wrinkles was made by making a simulated silicon plate, and the state of the wrinkles at the designated area was measured with a VISMETOMETER, an image analyzer.

The state of the wrinkles at the designated site was measured before and after the experiment of 8 weeks and the experiment was performed. The average value of the parameter values after 8 weeks experiment minus the parameter values before the experiment is shown in Table 3 below. A negative value of the average value of the measurement means that the wrinkle improvement effect is higher.

Wrinkle improvement effect measurement group R1 ^* R2 ^** R3 ^*** R4 ⁺ R5 ⁺⁺ Control 1 0.04 0.03 0.01 -0.01 0.00 Control group 2 -0.04 -0.02 -0.01 0.01 -0.01 Control group 3 -0.05 -0.02 -0.02 -0.01 -0.01 Control group 4 -0.09 -0.05 -0.04 -0.02 -0.01 Experiment 1 -0.15 -0.10 -0.08 -0.04 -0.03 Experiment 2 -0.15 -0.12 -0.07 -0.05 -0.03 Experiment group 3 -0.20 -0.15 -0.08 -0.06 -0.05 Experiment group 4 -0.30 -0.21 -0.14 -0.10 -0.09

^* R1: Difference between the maximum value and the minimum value of the contour line.

^** R2: Average value of the R1 values among the five arbitrary wrinkle contour lines.

^*** R3: Peak value of R1 divided by 5.

⁺ R4: Average value of the baseline of the wrinkle contour minus the value of each apex and valley.

⁺⁺ R5: Average of the value obtained by subtracting each wrinkle contour from the baseline of the contour line.

As can be seen from the results of Table 3 above, it was confirmed that the test groups 1 to 4 showed more excellent improvement in the performance of the test than the control group 1 to the control group 4. Considering that the total ratio of type 3 collagen recombinant protein, type 3 collagen-derived peptide or Asian ticoside was constant at 0.3 wt%, except for the control group 1, the type 3 collagen recombinant protein, the type 3 Collagen-derived peptide, and Asian ticoside are used in combination, they have synergistic effect to improve wrinkles. Further, in the experimental group 4, in the case of using all of the three components in combination, the effect was 1.8 times to 3 times as much as that of the control groups 2 to 4, By confirming what is visible, it was confirmed that high synergy effect was obtained by using the composite.

Example 3. Measurement of the type 3 collagen synthesis promotion effect

In the present invention, the effect of enhancing the synthesis of type 3 collagen by the treatment of the type III collagen peptide and / or the antioxidant as an active ingredient of the cosmetic composition was measured. This was quantitatively measured using the Collagen Type III EIA assay. Specifically, cells of 1 x 10000 cells / well were cultured in a 24-well culture plate for 24 hours. Derived collagen-derived peptide and / or Asian ticoside at the concentrations listed in Table 4 were added thereto and treated for 48 hours. 100 μl of the culture supernatant was taken and the amount of type 3 collagen synthesis was measured using a Collagen Type III EIA kit. The enzyme immunoassay is a one step sandwich method in which a 96-well culture plate is first coated with an antibody that specifically binds to type 3 collagen, and a cell culture medium and an enzyme-labeled antibody are added thereto to perform a reaction, Adding a color to the reaction, stopping the reaction, and measuring the absorbance.

The absorbance at 450 nm wavelength was measured with an ELISA reader, and a graph was prepared with a standard type 3 collagen concentration. Quantitative measurement was carried out. The number of samples per one concentration was measured, and the results were averaged.

Increase in collagen biosynthesis Test substance and concentration Increase in collagen biosynthesis (RCB) (%) Control group 0 Type 3 collagen-derived peptide (10 ppm) 15 Iaitico side (10ppm) 30 Type 3 collagen-derived peptide (5 ppm) + Asian ticoside (5 ppm) 60

From the results of Table 4, it was confirmed that the composition of the present invention significantly increased the collagen biosynthesis amount due to the synergistic effects of the peptide of type 3 collagen and the amino acid ticoside at the same time.

From the above results, it was found that the cosmetic composition comprising at least two of the group consisting of the type III collagen recombinant protein, the type III collagen-derived peptide and the Asian ticoside exhibited remarkably excellent wrinkle-reducing effect than the composition comprising each of them alone In particular, in the case of using the combination of the peptide of type 3 collagen and the peptide of Asian ticoside, the amount of type 3 collagen biosynthesis was remarkably increased, and that of type 3 collagen recombinant protein, type 3 collagen peptide, and Asian ticoside It can be confirmed that the synergistic effect is greatest in terms of the effect of improving the wrinkles.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

<110> LG HOUSEHOLD & HEALTH CARE LTD. <120> Cosmetic composition for anti-wrinkle <130> PA120973KR <150> KR10-2012-0118155 <151> 2012-10-23 <160> 2 <170> Kopatentin 2.0 <210> 1 <211> 1466 <212> PRT <213> recombinant Type 3 Collagen <400> 1 Met Met Ser Phe Val Gln Lys Gly Ser Trp Leu Leu Leu Ala Leu Leu 1 5 10 15 His Pro Thr Ile Ile Leu Ala Gln Gln Glu Ala Val Glu Gly Gly Cys 20 25 30 Ser His Leu Gly Gln Ser Tyr Ala Asp Arg Asp Val Trp Lys Pro Glu 35 40 45 Pro Cys Gln Ile Cys Val Cys Asp Ser Gly Ser Val Leu Cys Asp Asp 50 55 60 Ile Ile Cys Asp Asp Gln Glu Leu Asp Cys Pro Asn Pro Glu Ile Pro 65 70 75 80 Phe Gly Glu Cys Cys Ala Val Cys Pro Gln Pro Pro Thr Ala Pro Thr 85 90 95 Arg Pro Pro Asn Gly Gln Gly Pro Gln Gly Pro Lys Gly Asp Pro Gly 100 105 110 Pro Pro Gly Ile Pro Gly Arg Asn Gly Asp Pro Gly Ile Pro Gly Gln 115 120 125 Pro Gly Ser Pro Gly Ser Pro Gly Pro Pro Gly Ile Cys Glu Ser Cys 130 135 140 Pro Thr Gly Pro Gln Asn Tyr Ser Pro Gln Tyr Asp Ser Tyr Asp Val 145 150 155 160 Lys Ser Gly Val Ala Val Gly Gly Leu Ala Gly Tyr Pro Gly Pro Ala 165 170 175 Gly Pro Pro Gly Pro Pro Gly Pro Pro Gly Thr Ser Gly His Pro Gly 180 185 190 Ser Pro Gly Ser Pro Gly Tyr Gln Gly Pro Pro Gly Glu Pro Gly Gln 195 200 205 Ala Gly Pro Ser Gly Pro Pro Gly Pro Pro Gly Ala Ile Gly Pro Ser 210 215 220 Gly Pro Ala Gly Lys Asp Gly Glu Ser Gly Arg Pro Gly Arg Pro Gly 225 230 235 240 Glu Arg Gly Leu Pro Gly Pro Pro Gly Ile Lys Gly Pro Ala Gly Ile 245 250 255 Pro Gly Phe Pro Gly Met Lys Gly His Arg Gly Phe Asp Gly Arg Asn 260 265 270 Gly Glu Lys Gly Gly Thr Gly Ala Pro Gly Leu Lys Gly Gly Asn Gly 275 280 285 Leu Pro Gly Glu Asn Gly Ala Pro Gly Pro Met Gly Pro Arg Gly Ala 290 295 300 Pro Gly Glu Arg Gly Arg Pro Gly Leu Pro Gly Ala Ala Gly Ala Arg 305 310 315 320 Gly Asn Asp Gly Ala Arg Gly Ser Asp Gly Gln Pro Gly Pro Pro Gly 325 330 335 Pro Pro Gly Thr Ala Gly Phe Pro Gly Ser Pro Gly Ala Lys Gly Glu 340 345 350 Val Gly Pro Ala Gly Ser Pro Gly Ser Asn Gly Ala Pro Gly Gln Arg 355 360 365 Gly Glu Pro Gly Pro Gln Gly His Ala Gly Ala Gln Gly Pro Pro Gly 370 375 380 Pro Pro Gly Ile Asn Gly Ser Pro Gly Gly Lys Gly Glu Met Gly Pro 385 390 395 400 Ala Gly Ile Pro Gly Ala Pro Gly Leu Met Gly Ala Arg Gly Pro Pro 405 410 415 Gly Pro Ala Gly Ala Asn Gly Ala Pro Gly Leu Arg Gly Gly Ala Gly 420 425 430 Glu Pro Gly Lys Asn Gly Ala Lys Gly Glu Pro Gly Pro Arg Gly Glu 435 440 445 Arg Gly Glu Ala Gly Ile Pro Gly Val Pro Gly Ala Lys Gly Glu Asp 450 455 460 Gly Lys Asp Gly Ser Pro Gly Glu Pro Gly Ala Asn Gly Leu Pro Gly 465 470 475 480 Ala Ala Gly Glu Arg Gly Ala Pro Gly Phe Arg Gly Pro Ala Gly Pro 485 490 495 Asn Gly Ile Pro Gly Glu Lys Gly Pro Ala Gly Glu Arg Gly Ala Pro 500 505 510 Gly Pro Ala Gly Pro Arg Gly Ala Ala Gly Glu Pro Gly Arg Asp Gly 515 520 525 Val < / RTI > Pro Gly Gly Pro Gly Met Gly Gly Met Pro Gly Ser Pro Gly Gly 530 535 540 Pro Gly Ser Asp Gly Lys Pro Gly Pro Pro Gly Ser Gln Gly Gly Ser 545 550 555 560 Gly Arg Pro Gly Pro Pro Gly Pro Ser Gly Pro Arg Gly Gln Pro Gly 565 570 575 Val Met Gly Phe Pro Gly Pro Lys Gly Asn Asp Gly Ala Pro Gly Lys 580 585 590 Asn Gly Glu Arg Gly Gly Pro Gly Gly Pro Gly Pro Gln Gly Pro Pro 595 600 605 Gly Lys Asn Gly Glu Thr Gly Pro Gln Gly Pro Pro Gly Pro Thr Gly 610 615 620 Pro Gly Gly Asp Lys Gly Asp Thr Gly Pro Pro Gly Pro Gln Gly Leu 625 630 635 640 Gln Gly Leu Pro Gly Thr Gly Gly Pro Pro Gly Gly Asn Gly Lys Pro 645 650 655 Gly Glu Pro Gly Pro Lys Gly Asp Ala Gly Ala Pro Gly Ala Pro Gly 660 665 670 Gly Lys Gly Asp Ala Gly Ala Pro Gly Glu Arg Gly Pro Pro Gly Leu 675 680 685 Ala Gly Ala Pro Gly Leu Arg Gly Gly Ala Gly Pro Pro Gly Pro Glu 690 695 700 Gly Gly Lys Gly Ala Ala Gly 705 710 715 720 Thr Pro Gly Leu Gln Gly Met Pro Gly Glu Arg Gly Gly Leu Gly Ser 725 730 735 Pro Gly Pro Lys Gly Asp Lys Gly Gly Pro Gly Gly Pro Gly Ala Asp 740 745 750 Gly Val Pro Gly Lys Asp Gly Pro Arg Gly Pro Thr Gly Pro Ile Gly 755 760 765 Pro Pro Gly Pro Ala Gly Gln Pro Gly Asp Lys Gly Gly Gly Gly Ala 770 775 780 Pro Gly Leu Pro Gly Ile Ala Gly Pro Arg Gly Ser Pro Gly Gly Arg 785 790 795 800 Gly Glu Thr Gly Pro Gly Pro Gly Aly Gly Phe Pro Gly Ala Pro Gly 805 810 815 Gln Asn Gly Glu Pro Gly Gly Lys Gly Glu Arg Gly Ala Pro Gly Glu 820 825 830 Lys Gly Glu Gly Gly Pro Pro Gly Val Ala Gly Pro Pro Gly Gly Ser 835 840 845 Gly Pro Ala Gly Pro Pro Gly Pro Gly Gly Val Gly Gly Gly Arg Gly 850 855 860 Ser Pro Gly Gly Pro Gly Ala Gly Phe Pro Gly Ala Arg Gly Leu 865 870 875 880 Pro Gly Pro Pro Gly Ser Asn Gly Asn Pro Gly Pro Pro Gly Pro Ser 885 890 895 Gly Ser Pro Gly Lys Asp Gly Pro Pro Gly Pro Ala Gly Asn Thr Gly 900 905 910 Ala Pro Gly Ser Pro Gly Val Ser Gly Pro Lys Gly Asp Ala Gly Gln 915 920 925 Pro Gly Glu Lys Gly Ser Pro Gly Ala Gln Gly Pro Pro Gly Ala Pro 930 935 940 Gly Pro Leu Gly Ile Ala Gly Ile Thr Gly Ala Arg Gly Leu Ala Gly 945 950 955 960 Pro Pro Gly Met Pro Gly Pro Arg Gly Ser Pro Gly Pro Gln Gly Val 965 970 975 Lys Gly Glu Ser Gly Lys Pro Gly Ala Asn Gly Leu Ser Gly Glu Arg 980 985 990 Gly Pro Pro Gly Pro Gly Gly Leu Pro Gly Leu Ala Gly Thr Ala Gly 995 1000 1005 Glu Pro Gly Arg Asp Gly Asn Pro Gly Ser Asp Gly Leu Pro Gly Arg 1010 1015 1020 Asp Gly Ser Pro Gly Gly Lys Gly Asp Arg Gly Glu Asn Gly Ser Pro 1025 1030 1035 1040 Gly Ala Pro Gly Ala Pro Gly His Pro Gly Pro Pro Gly Pro Val Gly 1045 1050 1055 Pro Ala Gly Lys Ser Gly Asp Arg Gly Glu Ser Gly Pro Ala Gly Pro 1060 1065 1070 Ala Gly Ala Pro Gly Pro Ala Gly Ser Arg Gly Ala Pro Gly Pro Gln 1075 1080 1085 Gly Pro Arg Gly Asp Lys Gly Glu Thr Gly Glu Arg Gly Ala Ala Gly 1090 1095 1100 Ile Lys Gly His Arg Gly Phe Pro Gly Asn Pro Gly Ala Pro Gly Ser 1105 1110 1115 1120 Pro Gly Pro Ala Gly Gln Gln Gly Ala Ile Gly Ser Pro Gly Pro Ala 1125 1130 1135 Gly Pro Arg Gly Pro Val Gly Pro Ser Gly Pro Pro Gly Lys Asp Gly 1140 1145 1150 Thr Ser Gly His Pro Gly Pro Ile Gly Pro Pro Gly Pro Arg Gly Asn 1155 1160 1165 Arg Gly Glu Arg Gly Ser Glu Gly Ser Pro Gly His Pro Gly Gln Pro 1170 1175 1180 Gly Pro Pro Gly Pro Pro Gly Ala Pro Gly Pro Cys Cys Gly Gly Val 1185 1190 1195 1200 Gly Ala Ala Ala Aly Gly Ily Gly Gly Gly Gly Lys Ala Gly Gly Phe 1205 1210 1215 Ala Pro Tyr Tyr Gly Asp Glu Pro Met Asp Phe Lys Ile Asn Thr Asp 1220 1225 1230 Glu Ile Met Thr Ser Leu Lys Ser Val Asn Gly Gln Ile Glu Ser Leu 1235 1240 1245 Ile Ser Pro Asp Gly Ser Arg Lys Asn Pro Ala Arg Asn Cys Arg Asp 1250 1255 1260 Leu Lys Phe Cys His Pro Glu Leu Lys Ser Gly Glu Tyr Trp Val Asp 1265 1270 1275 1280 Pro Asn Gln Gly Cys Lys Leu Asp Ala Ile Lys Val Phe Cys Asn Met 1285 1290 1295 Glu Thr Gly Glu Thr Cys Ile Ser Ala Asn Pro Leu Asn Val Pro Arg 1300 1305 1310 Lys His Trp Trp Thr Asp Ser Ser Ala Glu Lys Lys His Val Trp Phe 1315 1320 1325 Gly Glu Ser Met Asp Gly Gly Phe Gln Phe Ser Tyr Gly Asn Pro Glu 1330 1335 1340 Leu Pro Glu Asp Val Leu Asp Val Gln Leu Ala Phe Leu Arg Leu Leu 1345 1350 1355 1360 Ser Ser Arg Ala Ser Gln Asn Ile Thr Tyr His Cys Lys Asn Ser Ile 1365 1370 1375 Ala Tyr Met Asp Gln Ala Ser Gly Asn Val Lys Lys Ala Leu Lys Leu 1380 1385 1390 Met Gly Ser Asn Glu Gly Glu Phe Lys Ala Glu Gly Asn Ser Lys Phe 1395 1400 1405 Thr Tyr Thr Val Leu Glu Asp Gly Cys Thr Lys His Thr Gly Glu Trp 1410 1415 1420 Ser Lys Thr Val Phe Glu Tyr Arg Thr Arg Lys Ala Val Arg Leu Pro 1425 1430 1435 1440 Ile Val Asp Ile Ala Pro Tyr Asp Ile Gly Gly Pro Asp Gln Glu Phe 1445 1450 1455 Gly Val Asp Val Gly Pro Val Cys Phe Leu 1460 1465 <210> 2 <211> 3 <212> PRT <213> Type 3 Collagen derived peptide <220> <221> SITE <222> (2) &Lt; 223 > Xaa = Hydroxyproline <400> 2 Pro Xaa Gly One

Claims

Type 3 collagen recombinant protein, type 3 collagen derived peptide, and at least one selected from the group consisting of Asian thiosides.

The cosmetic composition according to claim 1, wherein the cosmetic composition comprises a peptide derived from type 3 collagen, and an Asian ticoside.

The cosmetic composition according to claim 1, wherein the cosmetic composition comprises a type 3 collagen recombinant protein, a type 3 collagen derived peptide, and an asiaticoside.

The cosmetic composition according to claim 1, wherein the cosmetic composition promotes the synthesis of type 3 collagen.

The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of improving wrinkles or improving skin elasticity.

The cosmetic composition according to claim 1, wherein the third type collagen-derived peptide is composed of proline, hydroxyproline, and glycine.

7. The cosmetic composition according to claim 6, wherein the third type collagen-derived peptide has a repeating sequence of a tripeptide of SEQ ID NO: 2.

The cosmetic composition according to claim 6, wherein the third type collagen-derived peptide is (proline-hydroxyproline-glycine) n and n is 30 to 70.

The composition of claim 1, wherein the composition is selected from the group consisting of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening longevity pack, a pack, a softener, a latex, a makeup base, an essence, Characterized in that it has a formulation selected from the group consisting of suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. Cosmetic composition.

The cosmetic composition of claim 1, further comprising a cosmetically acceptable carrier.

The cosmetic composition according to claim 1, further comprising a skin wrinkle improving component or a skin elasticity improving component.

A method for preventing or improving wrinkles of the skin or improving skin elasticity, comprising applying the cosmetic composition of any one of claims 1 to 11 to the skin of the individual.

Type 3 collagen recombinant protein, type 3 collagen peptide, and at least one member selected from the group consisting of Asian ticoside.

Wherein the composition comprises at least two selected from the group consisting of collagen type III collagen protein, type III collagen peptide, and Asian thioside.

A type III collagen recombinant protein, a type III collagen derived peptide, and an anticholinergic agent.