KR102204367B1 - Cosmetic compositions for improving skin wrinkle - Google Patents

Abstract

The present invention is a composition for promoting collagen synthesis comprising a compound represented by the following general formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient; Cosmetic composition and quasi-drug composition for preventing or improving skin wrinkles comprising the composition as an active ingredient; It relates to a pharmaceutical composition for preventing or treating skin wrinkles.
[General Formula 1]
Sialic acid-galactose-glucose
The composition of the present invention is very effective in improving skin elasticity, improving skin wrinkles, and inhibiting skin aging by promoting collagen synthesis of fibroblasts in the skin.

Description

Cosmetic compositions for improving skin wrinkle

The present invention is a composition for promoting collagen synthesis comprising a compound represented by the following general formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient; Cosmetic composition and quasi-drug composition for preventing or improving skin wrinkles comprising the composition as an active ingredient; It relates to a pharmaceutical composition for preventing or treating skin wrinkles.

[General Formula 1]

Sialic acid-galactose-glucose

Collagen, a major component of the extracellular matrix, is a major matrix protein produced in fibroblasts of the skin and is present in the extracellular stroma. In addition, it is an important protein that accounts for about 30% of the total weight of a biological protein and has a solid triple helix structure. Collagen forms most of the organic substances in skin, tendons, bones and teeth, especially in bones and skin (dermis). In most other sieve structures, they exist as fibrous inclusion bodies.

Collagen is a relatively weak immunogen, partly due to the masking of latent antigenic determinants by the helix structure of collagen, and this helix structure also makes collagen resistant to proteolysis. The main functions of collagen are known for its mechanical firmness, connective tissue resistance and tissue bonding, support of cell adhesion, and induction of cell division and differentiation (when the growth of organisms or wound healing), etc. (Van der Rest, etc., 1990). This collagen is reduced by age and photoaging caused by ultraviolet irradiation, which is known to be closely related to the formation of wrinkles in the skin (Arthur K. Balin et al., "Aging and the skin", 1989). Also, in recent years, as extensive studies on skin aging have been conducted, the important function of collagen in the skin has been revealed.

Conventionally, products containing collagen have been released in cosmetics to take advantage of the skin moisturizing effect of collagen, but these cosmetics, etc., apply collagen to the surface of the skin. It couldn't be said that it has an essential skin wrinkle improvement effect.

In order to solve this problem, interest in collagen synthesis promoting substances has increased, and conventionally known collagen synthesis promoting substances include vitamin C, retinoic acid, and transforming growth factor (TGF) (Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974), protein derived from animal negligence (Japanese Laid-Open Patent Publication No. 1996-231370), betulinic acid (Japanese Laid-Open Patent Publication No. 1996-208424) ), chlorella extract (Japanese Laid-Open Patent Publication No. 1997-040523, Japanese Laid-Open Patent Publication No. 1998-036283), and the like.

However, when these substances are applied to the skin, due to safety problems such as irritation and redness, the amount of use of these substances is limited or the effect is insignificant. Therefore, there is an urgent need for the development of a new collagen synthesis promoting material that is safer and more effective than the existing collagen synthesis promoting material.

Accordingly, the present inventors were developing a substance having an excellent collagen synthesis promoting effect as an active ingredient of a composition for improving skin wrinkles, while the compound represented by the following general formula 1 or a pharmaceutically acceptable salt thereof has a strong collagen synthesis promoting effect. It was found that the present invention was completed. There have been no previous studies on the effect of promoting collagen synthesis and improving skin wrinkles of the compound, which is an active ingredient of the present invention.

[General Formula 1]

Sialic acid-galactose-glucose

Japanese Laid-Open Patent Publication No. 1996-231370 Japanese Laid-Open Patent Publication No. 1996-208424 Japanese Published Patent Publication No. 1997-040523 Japanese Published Patent Publication No. 1998-036283

One object of the present invention is to provide a composition for promoting collagen synthesis comprising a compound represented by the following general formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.

[General Formula 1]

Sialic acid-galactose-glucose

Another object of the present invention is to provide a cosmetic composition for preventing or improving skin wrinkles comprising the composition as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating skin wrinkles comprising the composition for promoting collagen synthesis as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition for preventing or improving skin wrinkles comprising the composition for promoting collagen synthesis as an active ingredient.

Another object of the present invention is to provide a method for preventing or improving skin wrinkles comprising administering the composition for promoting collagen synthesis to an individual.

In order to solve the above problems, the present invention provides a composition for promoting collagen synthesis comprising a compound represented by the following general formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.

[General Formula 1]

Sialic acid-galactose-glucose

The term "sialic acid" of the present invention is a generic term for the N-acyl form of neuramic acid and its derivatives. There are N-acetylneuraminic acid (Neu5Ac) and N-glycolneuraminic acid (Neu5Gc), and there are derivatives in which the hydroxyl group is modified with an acetyl group, a sulfuric acid group, and a phosphoric acid group. Certain sugar chains, including sialic acid, have been shown to be involved in cell adhesion and cell recognition.

Preferably, the sialic acid in the present invention may be N-acetylneuraminic acid or N-glycolneuraminic acid, more preferably N-acetylneuraminic acid.

In the compound of Formula 1, sialic acid may be bound to galactose in various ways, and preferably, may be bound to galactose by α-2,3 bond or α-2,6 bond. More preferably, it may be bound to galactose by α-2,3 bond.

Also, the sialic acid may be a modified sialic acid. For example, the OH group on carbon 4 of the sialic acid may be modified (eg, substituted with a C1-C4 alkyl group).

In the compound of Formula 1, galactose and glucose, which are monosaccharides, may have a D- or L-form stereoisomer, and preferably may be a D-form stereoisomer.

The galactose and glucose may be linked by a glycosidic linkage, and the glycosidic bond may be an α- or β-glycosidic bond, but preferably a β-glycosidic bond.

According to a preferred embodiment of the present invention, the compound of Formula 1 may be sialyl lactose. Sialyl lactose is a compound in which galactose bound to sialic acid and glucose are linked by β-1,4 glycosidic bonds.

According to a preferred embodiment of the present invention, the sialyl lactose of the present invention is 2,3-sialyllactose: Neu5Ac-a-2-3-Gal-b-1-4-Glc, NANA- Lactose, 3'-N-Acetylneuraminyl-D-lactose) or 2,6-sialyllactose (2,6-sialyllactose: Neu5Ac-a-2-6-Gal-b-1-4-Glc) may be, Preferably, it may be 2,3-sialyl lactose. 2,6-sialylactose is an isomer of 2,3-sialyllactose.

The terms of the present invention "2,3-sialyllactose: Neu5Ac-a-2-3-Gal-b-1-4-Glc, NANA-Lactose, 3'-N-Acetylneuraminyl-D- lactose)" is a type of sialic sugar that exists on the surface of various animal cells and plays an important role in cell recognition, recognition of bacteria and viruses, and adsorption. 2,3-sialylactose in its natural state is It is an ingredient that is present in large amounts in breast milk. There may be several ways to obtain 2,3-sialyllactose. For example, 2,3-sialyl lactose may be obtained from the precursor through an enzyme synthesis method using a sugar transfer enzyme derived from a specific microorganism, but is not limited thereto. The experiment on 2,3-sialyl lactose presented in the present invention was conducted using 2,3-sialyl lactose purchased from Jinchem. The 2,3-sialyl lactose is represented by the following formula (1).

[Formula 1]

In the composition for promoting collagen synthesis of the present invention, the compound of the general formula 1 or a pharmaceutically acceptable salt thereof may be included in an amount of 0.000001% to 10% by weight, preferably 0.001% to 10% by weight It may be included in %, more preferably 0.1% to 10% by weight. When the content is less than 0.000001% by weight, a distinct effect cannot be expected, and when it exceeds 10% by weight, an increase in the effect cannot be achieved compared to an increase in the amount contained.

In the present invention, the term "collagen" refers to a group of naturally occurring proteins found in animals, particularly in the skin and connective tissues of vertebrates. It is a major component of connective tissue and is the most abundant protein in mammals, and accounts for 25 to 35% of the total biological protein. Collagen is an elongated fibrous form, mainly observed in fibrous tissues such as tendons, ligaments, and skin, and is abundant in cornea, cartilage, bone, blood vessels, gut, and intervertebral disk. . Fibroblasts are well known as cells that produce collagen. When the skin is aged or exposed to UV rays or heat, the ability to synthesize collagen decreases and the amount of collagen decreases rapidly. When the amount of collagen decreases, the shape of the muscles cannot be maintained and skin aging, skin elasticity weakened, and wrinkles occur.

That is, when the synthesis of collagen in skin fibroblasts is promoted, skin wrinkles are prevented or improved, skin elasticity is improved, skin regeneration, wound healing, damaged skin tissue repair and regeneration; And it is possible to obtain effects such as skin aging prevention.

In an embodiment of the present invention, 2,3-sialyl lactose and transforming growth factor-β (TGF-β: Transforming Growth Factor-beta) as a positive control were added to the culture medium of human fibroblasts. Addition, the degree of collagen biosynthesis was measured. As a result, 2,3-sialyllactose has excellent collagen biosynthesis ability against human-derived fibroblasts, and has an excellent collagen synthesis effect compared to the case of applying the transforming growth factor-β, which is generally known to have excellent collagen synthesis ability. It was confirmed that can be obtained (Table 2). Therefore, since the composition has a strong ability to promote collagen synthesis, it can be seen that it can be very useful as a skin wrinkle improvement agent, wound healing agent, or anti-aging agent.

As another embodiment of the present invention, it provides a cosmetic composition for preventing or improving skin wrinkles comprising the composition for promoting collagen synthesis as an active ingredient.

In the present invention, the term "skin wrinkles" refers to fine lines caused by deterioration of the skin, and may be caused by genes, a decrease in collagen present in the skin dermis, an external environment, and the like.

In the present invention, "skin wrinkle improvement" refers to suppressing or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been generated.

In a specific embodiment of the present invention, a cosmetic essence for improving skin wrinkles containing 2,3-sialylactose was prepared (Example 3), and skin wrinkle improvement of the essence for healthy women aged 35 to 50 years old The effect was tested. As a result, it was found through a questionnaire of the subject that the skin wrinkle improvement effect was excellent when the essence was used (Table 4), and the density of the skin wrinkles was compared to the cosmetic composition of Comparative Example 1 when the essence was applied through image analysis. It was found that it was greatly reduced (Table 5). Therefore, it was recognized that the cosmetic composition for improving skin wrinkles containing 2,3-sialylactose exhibits very excellent skin wrinkle improvement effects on fibroblasts of the skin.

The term "prevention" as used in the present invention refers to any action that suppresses or delays the occurrence of skin wrinkles by administering the composition of the present invention to an individual.

As used herein, the term "improvement" refers to any action that at least reduces the severity of a parameter related to the condition being treated, for example, symptoms.

The cosmetic composition for preventing or improving skin wrinkles according to the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, It may be formulated as soap, surfactant-containing cleansing, oil, powder foundation, emulsion, foundation, wax foundation, and spray, but is not limited thereto. More specifically, external ointment, foam, lotion, soft lotion, nutrition lotion, cream, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, emulsion, foundation, makeup base, soap , Liquid detergent, bath agent, sunscreen cream, sun oil, spray or powder may be prepared in the formulation, but is not limited thereto.

In addition, the cosmetic composition of the present invention may additionally contain one or more cosmetically acceptable carriers that are blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation. When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbons, propane / May contain propellants such as butane or dimethyl ether.

In addition, when the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butyl glycol oil, and in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan, etc. can be used. have.

In addition, when the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

In addition, when the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

In addition, as another embodiment of the present invention, it provides a pharmaceutical composition for preventing or treating skin wrinkles comprising the composition for promoting collagen synthesis as an active ingredient.

The term "treatment", as used herein, refers to any action to improve or benefit from symptoms of skin wrinkles by administering the composition of the present invention to an individual.

In addition to including the composition for promoting collagen synthesis as an active ingredient, the pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier.

In the present invention, the "pharmaceutically acceptable" means that it is commonly used in the field of pharmaceuticals, which does not stimulate an organism when administered, and does not inhibit the biological activity and properties of the administered compound.

The pharmaceutical composition of the present invention can be formulated with the carrier and used as a pharmaceutical, feed additive, drinking water additive, and the like.

In the present invention, the kind of the carrier is not particularly limited, and any carrier commonly used in the art may be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, ethanol, and the like. I can. These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention may be used by adding other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostatic agents, if necessary, and fillers, extenders, wetting agents, disintegrants, dispersants, surfactants , A binder or a lubricant may be additionally added and used.

The pharmaceutical composition of the present invention may be formulated and used in various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of the formulation for oral administration include troches, lozenges, tablets, aqueous suspensions, oily suspensions, powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs, etc. Can be mentioned.

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricating oils such as magnesium stearate, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax can be used, and sweeteners, fragrances, and syrups can also be used. I can.

Furthermore, in the case of capsules, in addition to the above-mentioned substances, a liquid carrier such as fatty oil may be additionally used.

Non-limiting examples of the parenteral preparation include injection solutions, suppositories, respiratory inhalation powders, spray aerosols, ointments, coating powders, oils, creams, and the like.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, external preparations, etc. may be used. As the non-aqueous solvents and suspensions, propylene glycol, polyethylene Glycols, vegetable oils such as olive oil, injectable esters such as ethyloleate, and the like may be used.

In addition, more specifically, when the pharmaceutical composition of the present invention is formulated as an injection solution, the composition of the present invention is prepared as a solution or suspension by mixing in water with a stabilizer or a buffer, and this is used in an ampoule or vial. It can be formulated for unit administration. In addition, when the pharmaceutical composition of the present invention is formulated with an aerosol, a propellant or the like may be blended with an additive so that the aqueous concentrated or wet powder is dispersed.

In addition, when the pharmaceutical composition of the present invention is formulated as an ointment, cream, etc., animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide It can be formulated using the like as a carrier.

The pharmaceutically effective amount and effective dosage of the pharmaceutical composition of the present invention may vary depending on the formulation method, the mode of administration, the administration time and/or the route of administration of the pharmaceutical composition, and the response to be achieved by the administration of the pharmaceutical composition Including the type and degree, the type of the subject to be administered, the age, weight, general health condition, the symptom or degree of the disease, sex, diet, excretion, drugs used concurrently with the subject or at the same time, and other components of the composition. It may vary depending on various factors and similar factors well known in the medical field, and a person of ordinary skill in the art can easily determine and prescribe an effective dosage for a desired treatment.

The dosage for a more preferable effect of the pharmaceutical composition of the present invention may be preferably 0.01 mg/kg to 100,000 mg/kg per day, more preferably 10 mg/kg to 500 mg/kg. The administration of the pharmaceutical composition of the present invention may be administered once a day, or may be administered several times. Therefore, the dosage does not limit the scope of the present invention in any way.

The route of administration and the method of administration of the pharmaceutical composition of the present invention may each be independent, and the method is not particularly limited, and any route of administration and administration method may be applied as long as the pharmaceutical composition can reach the desired site. You can follow. The pharmaceutical composition may be administered orally or parenterally.

As the method of parenteral administration, for example, intravenous administration, intraperitoneal administration, intramuscular administration, topical application, transdermal administration, or subcutaneous administration may be used, and the composition is applied to a diseased site, sprayed, or inhaled. The method can also be used, but is not limited thereto. Preferably, the composition may be applied to the diseased area.

As another embodiment of the present invention, there is provided a quasi-drug composition for preventing or improving skin wrinkles comprising the composition for promoting collagen synthesis as an active ingredient.

The quasi-drug composition of the present invention is used as a body cleanser, disinfectant cleaner, detergent, kitchen cleaner, cleaning cleaner, toothpaste, gargle, wet tissue, detergent, soap, hand wash, hair cleaner, hair softener, humidifier filler, mask, ointment and filter filler. It can be prepared in a formulation selected from the group consisting of, but is not limited thereto.

As another embodiment of the present invention, there is provided a method for preventing or improving skin wrinkles comprising administering the composition for promoting collagen synthesis to an individual.

As used herein, the term "individual" refers to all animals including mammals including mice, livestock, humans, and the like.

In the method for preventing or improving skin wrinkles of the present invention, the dosage, route of administration, and mode of administration of the composition for promoting collagen synthesis are the same as described above with respect to the pharmaceutical composition of the present invention.

The cosmetic composition for promoting collagen synthesis of the present invention includes a compound represented by the following General Formula 1 as a collagen synthesis promoter or a pharmaceutically acceptable salt thereof, thereby promoting collagen synthesis of fibroblasts of the skin, thereby improving skin elasticity, It improves skin wrinkles and has an excellent effect of inhibiting skin aging.

[General Formula 1]

Sialic acid-galactose-glucose

Hereinafter, examples, etc. will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1. Cytotoxicity test

In order to verify the primary safety as a raw material used in cosmetics, V79-cells (continuous cell line of Chinese hamster, lung tissue fibroblasts) were cultured against 2,3-sialylactose, and the MTT method (Mossman T. (1983) Rapid Colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays.Journal of Immunological Methods 65, 55-63) was performed to evaluate cytotoxicity, and the results are shown in Table 1 below.

Cytotoxicity test result of 2,3-sialyllactose sample IC ₅₀ (%, W/V) Sodium lauryl sulfate (SLS) 0.006 2,3-sialyllactose 5.89

In Table 1, IC ₅₀ means the concentration of killing 50% of cells with Inhibitory Concentration 50.

As shown in Table 1, the IC ₅₀ value of 2,3-sialyl lactose of the present invention was 5.89, indicating that the cytotoxicity was more than 900 times lower than that of sodium lauryl sulfate, indicating excellent safety.

Example 2. 2,3- Sialyllactose Collagen biosynthesis promotion effect

By adding 2,3-sialyl lactose to the culture medium of human-derived fibroblasts, the effect of a cosmetic for improving skin wrinkles at the cellular level was tested. Biosynthetic collagen was measured using PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

Transforming Growth Factor-β (TGF-β: Transforming Growth Factor-beta), which is a positive control, by making 2,3-sialylactose at a final concentration of 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, and 10 μg/ml And a negative control group, respectively, added to the culture medium of human fibroblasts and cultured for 1 day, and then the culture solution was taken and the degree of collagen biosynthesis at each concentration was measured at 450 nm using a PICP EIA KIT. . The amount of collagen production was measured as UV absorbance (Abs), and the rate of increase in collagen biosynthesis was calculated as a% value of the relative synthesis ability with respect to the negative control (no addition) and summarized in Table 2.

Collagen biosynthesis effect at the cellular level according to concentration Added sample Application concentration (μg/ml) Collagen production amount (Abs) Increase rate (%) Control - 185.98 - 2,3-sialyllactose 0.01 219.1 18 0.1 268.42 44 One 287.78 55 10 286.58 54 Transgenic growth factor-β 0.01 228.20 23

As can be seen from the results of Table 2, 2,3-sialyllactose has excellent collagen biosynthesis promoting ability for human-derived fibroblasts, and in general, when applying the transforming growth factor-β, which is known to have excellent collagen synthesis promoting ability Compared with, it was found that an excellent collagen synthesis promoting effect can be obtained.

Example 3 and Comparative example 1. For skin wrinkle improvement Cosmetic Essence Manufacturing and skin wrinkle improvement effect

1) Skin wrinkle improvement Cosmetic Essence Produce

A cosmetic essence for improving skin wrinkles containing 2,3-sialyl lactose was prepared (Example 3), and in the same manner as in Example 3, Comparative Example 1 was prepared, except that 2,3-sialyl lactose was not included. A cosmetic essence was prepared. The detailed composition is shown in Table 3 below.

Composition water Example 3 (% by weight) Comparative Example 1 (% by weight) 2,3-sialyllactose One - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense 0.05 0.05 Purified water to 100 to 100

2) Skin wrinkle improvement effect experiment through panel test

With respect to the essences of Example 3 and Comparative Example 1, the skin wrinkle improvement effect was tested for healthy women aged 35 to 50 years old as follows.

30 women aged 35 to 50 years old were divided into two groups of 15 each, group 1 was the essence of Example 3 containing 1% by weight of 2,3-sialyllactose, and the second group was the essence of Comparative Example 1. Was applied to the face part once a day for 3 months.

After 3 months, the degree of improvement of skin wrinkles was evaluated through a questionnaire of subjects and image analysis of skin wrinkles. The subject's questionnaire was evaluated in three stages: no improvement, slight improvement, and significant improvement compared to before use for skin wrinkle improvement and elasticity improvement, and the results are shown in Table 4.

Skin wrinkle improvement effect of Example 3 according to the present invention (persons) sample No improvement Slight improvement Significant improvement Comparative Example 1 8 6 One Example 3 - 9 6

As can be seen in Table 4, it was found that when the essence of Example 3 according to the present invention was used, the effect of improving skin wrinkles was superior to that of the cosmetic composition of Comparative Example 1.

3) Wrinkle improvement effect experiment through image analysis

For the evaluation of wrinkles by image analysis, a replica under the eyes was collected before the experiment was started (Xantopren, Bayer), and a replica was collected from the same area under the eyes immediately after the experiment was completed. The density of skin wrinkles was measured by dimensional analysis. The measurement results of the skin wrinkle density by image analysis are shown in Table 5 by averaging the reduction rate of the skin wrinkle density after use compared to the skin wrinkle density before use.

Skin wrinkle improvement effect (image analysis) sample Skin wrinkle density reduction rate (%) Comparative Example 1 8 Example 3 36

As can be seen from Table 5, when the essence of Example 3 to which 2,3-sialyl lactose was added according to the present invention was applied, the density of skin wrinkles was significantly reduced compared to the cosmetic composition of Comparative Example 1.

As described above, the cosmetic composition for improving skin wrinkles containing 2,3-sialyllactose as an active ingredient showed very excellent skin wrinkle improvement effect on fibroblasts of the skin.

Hereinafter, examples of preparation of a cosmetic composition for improving skin wrinkles, a quasi-drug composition, and a pharmaceutical composition for treating skin wrinkles containing 2,3-sialyl lactose are shown.

Example 4. Skin ointment for skin wrinkle improvement

A formulation of a skin ointment for skin wrinkle improvement containing 2,3-sialyl lactose was prepared in Example 4, and the detailed composition is shown in Table 6 below.

Composition water Example 4 (% by weight) 2,3-sialyllactose 2 Diethyl sebacate 8 Abiding 5 Polyoxyethylene oleyl ether phosphate 6 Sodium benzoate 0.1 Washelin to 100

Example 5. For skin wrinkle improvement Cosmetic cream

A formulation of a cosmetic cream for improving skin wrinkles containing 2,3-sialyl lactose was prepared in Example 5, and the detailed composition is shown in Table 7 below.

Composition water Example 5 (% by weight) 2,3-sialyllactose 0.5 Stearic acid 15.0 Cetanol 1.0 Potassium hydroxide 0.7 glycerin 5.0 Propylene glycol 3.0 antiseptic 0.05 incense 0.05 Purified water to 100

Example 6. Softening lotion for skin wrinkle improvement

A formulation of a softening lotion for skin wrinkle improvement containing 2,3-sialyl lactose was prepared in Example 6, and the detailed composition is shown in Table 8 below.

Composition water Example 6 (% by weight) 2,3-sialyllactose 0.2 ethanol 10.0 Polylaurate polyoxyethylene sorbitan 1.0 Methyl paraoxybenzoate 0.2 glycerin 5.0 1,3-butylene glycol 6.0 incense 0.05 Pigment 0.05 Purified water to 100

Example 7. Nourishing lotion for skin wrinkle improvement

A formulation of a nutrient lotion for improving skin wrinkles containing 2,3-sialyl lactose was prepared in Example 7, and the detailed composition is shown in Table 9 below.

Composition water Example 7 (% by weight) 2,3-sialyllactose 0.1 Wasserin 2.0 Sorbitan sesquioleate 0.8 Polyoxyethyleneoleylethyl 1.2 Methyl paraoxybenzoate 0.1 Propylene glycol 5.0 ethanol 3.2 Carboxyvinyl polymer 18.0 Potassium hydroxide 0.1 Pigment 0.05 incense 0.05 Purified water to 100

Example 8. Skin wrinkle improvement Cosmetic pack

A formulation of a cosmetic pack for improving skin wrinkles containing 2,3-sialyl lactose was prepared in Example 8, and the detailed composition is shown in Table 10 below.

Composition water Example 8 (% by weight) 2,3-sialyllactose 0.2 glycerin 5.0 Propylene glycol 4.0 Polyvinyl alcohol 15.0 ethanol 8.0 Polyoxyethyleneoleylethyl 1.0 Methyl paraoxybenzoate 0.2 Pigment 0.05 incense 0.05 Purified water to 100

Example 9. For skin wrinkle treatment Capsule

A capsule formulation for treatment of skin wrinkles containing 2,3-sialyl lactose was prepared in Example 9, and the detailed composition is shown in Table 11 below.

Composition water Example 9 (mg) 2,3-sialyllactose 10.0 Starch 10.0 Magnesium stearate 100.0

Example 10. Injection for skin wrinkle treatment

An injection formulation for treating skin wrinkles containing 2,3-sialyl lactose was prepared in Example 10, and the detailed composition is shown in Table 12 below.

Composition water Example 10 (mg) 2,3-sialyllactose 10.0 Acid sodium sulfite 10.0 Methylparaben 6.0 Furopenparaben 4.0 Monobasic sodium phosphate 12.0 Sodium diphosphate 8.0 Sodium hydroxide 10.0 Water for injection 10.0

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof.

Claims (10)

A cosmetic composition for promoting collagen synthesis comprising 2,3-sialyl lactose (Neu5Ac-α-2-3-Gal-β-1-4-Glc) or a pharmaceutically acceptable salt thereof as an active ingredient.
delete delete The method of claim 1, wherein the 2,3-sialyl lactose (Neu5Ac-α-2-3-Gal-β-1-4-Glc) or a pharmaceutically acceptable salt thereof is 0.000001% by weight based on the total weight of the composition. A cosmetic composition for promoting collagen synthesis, comprising to 10% by weight.
The method of claim 1, wherein the 2,3-sialyl lactose (Neu5Ac-α-2-3-Gal-β-1-4-Glc) or a pharmaceutically acceptable salt thereof is 0.1% by weight based on the total weight of the composition. A cosmetic composition for promoting collagen synthesis, comprising to 10% by weight.
The cosmetic composition of claim 1, wherein the composition is for preventing or improving skin wrinkles.
The method of claim 1, wherein the cosmetic composition is from the group consisting of ointment for external use, cream, foam, lotion, pack, softening water, emulsion, foundation, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream and sun oil. Cosmetic composition, characterized in that having a selected formulation.
delete A quasi-drug composition for promoting collagen synthesis comprising 2,3-sialyl lactose (Neu5Ac-α-2-3-Gal-β-1-4-Glc) or a pharmaceutically acceptable salt thereof as an active ingredient.
The quasi-drug composition of claim 9, wherein the composition is for preventing or improving skin wrinkles.