KR20090029536A - Composition for promoting collagen synthesis and cosmetics comprising the same - Google Patents

Abstract

A composition for promoting collagen synthesis containing prangenidin and genistein is provided to ensure excellent collagen formation promotion function and collagenase inhibiting function. A composition for promoting collagen synthesis comprises a compound of the chemical formula 1 and a compound of the chemical formula 2. The weight ratio of the compound of the chemical formula 1 and the compound of the chemical formula 2 is 1-100. The cosmetic composition has forms of powder agent, gel agent, aerosol propellant, ointments, creams, liquor, skin adhesion type formulation, lotion, pack, emulsion, foundation, makeup base, essence, soap, liquid washing, bath and sunscreen cream.

Description

Composition for promoting collagen synthesis and cosmetics comprising the same}

The present invention relates to a composition for promoting collagen synthesis and cosmetics for inhibiting and improving wrinkles comprising the same, and more specifically, a composition for promoting collagen synthesis, comprising frangenidine and genistein, and inhibiting wrinkles comprising the same. It relates to cosmetics for improvement.

Collagen is a major constituent of the extracellular matrix and is a major matrix protein produced in skin fibroblasts and is present in extracellular epilepsy. It is also an important protein, accounting for about 30% of the total weight of biological proteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bones and skin (dermis). Collagen also exists as a fibrous inclusion body in most other body structures.

Collagen is a relatively weak immunogen due to the shielding of latent antigenic determinants by the helix structure, which also makes collagen resistant to proteolysis. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissue, adhesion of tissue, support of cell adhesion, and induction of cell division and differentiation (in organic growth or wound healing) (Van der Rest et al. , Ann NY Acad Sci, 1990). This collagen is reduced by age and photoaging by UV irradiation, which is known to be closely associated with wrinkle formation of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and promotes collagen synthesis in the damaged epithelium so that wounds can be quickly recovered without scarring.

Conventionally, in order to take advantage of collagen's skin moisturizing effect and wound healing effect, products containing collagen in an external skin composition such as cosmetics or ointment have been released, but these products apply collagen itself to the surface of the skin and are collagen, which is a polymer material. Because it is difficult to absorb the percutaneous effect of moisturizing or wound healing effect can not be expected to show the function of essential skin function and wound healing.

Accordingly, interest in collagen synthesis promoters has increased, and known collagen synthesis promoters include vitamin C, retinoic acid and transforming growth factor (TGF), Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974).

In addition, Japanese Patent Application Laid-open No. Hei 8-231370 discloses a protein derived from an animal placenta as a collagen synthesis material, and Japanese Patent Laid-Open No. Hei 8-208424 discloses betulinic acid, and Japanese Patent Laid-Open No. 9- 40523 and 10-36283 disclose chlorella extracts and the like which exhibit fibroblast proliferation-promoting action.

However, the above-mentioned conventional materials are limited in usage due to safety problems such as irritation and redness during skin application, or the effects are insignificant, so that the effect of improving skin function or wound healing cannot be expected. Therefore, there is an urgent need for the development of a new skin topical composition that is safer and more effective in vivo than existing skin topical composition.

The present invention has been made to solve the above problems, and has excellent stability to the living body while exhibiting excellent collagen synthesis promoting effect, and excellent processability, such as solubility in a solvent to provide a composition for promoting collagen synthesis can be applied in a variety of formulations It aims to do it.

Accordingly, an object of the present invention is to provide a composition for promoting collagen synthesis, including frangenidine and genistein represented by a specific formula.

Another object of the present invention is to provide a cosmetic of various formulations comprising the composition for promoting collagen synthesis.

The present invention provides a composition for promoting collagen synthesis comprising a compound of formula 1 and a compound of formula 2 as a means for solving the above problems.

[Formula 1] [Formula 2]

The content of frangenidine represented by Formula 1 included in the composition of the present invention is preferably 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition.

The content of Zenistein represented by Formula 2 included in the composition of the present invention is preferably 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition.

The present invention provides a cosmetic for inhibiting and improving skin wrinkles comprising the composition for promoting collagen synthesis as another means for solving the above problems.

The composition for promoting collagen synthesis of the present invention is excellent in stability to the living body, exhibits an excellent collagen formation promoting effect can be applied to a variety of cosmetic or pharmaceutical uses. In particular, the composition of the present invention is applied to cosmetics exhibits excellent efficacy in suppressing and improving wrinkles.

The present invention relates to a composition for promoting collagen synthesis comprising frangenidine and genistein represented by a specific chemical formula. The composition of the present invention has an excellent collagen formation promoting ability and collagenase inhibitory ability (collagenase) ability, even when a small amount of the active ingredient has the advantage that the efficacy is excellent.

Hereinafter, the composition for promoting collagen synthesis of the present invention will be described in detail.

Frangenidine contained in the collagen synthesis promoting composition of the present invention is represented by the following formula (1).

[Formula 1]

Frangenidine represented by Formula 1 is safe for the living body and has an effect of promoting collagen synthesis of skin fibroblasts, and thus is suitable for use as a collagen synthesis promoter, and improves skin wrinkles and improves skin elasticity. The healing effect is very good.

Frangenidine is a compound mainly present in the hawthorn and roots of plants, and can be chemically synthesized by conventional methods known in the art, or extracted from plants and the like. For example, frangenidine can be chopped off the roots of white lumber and plant copper, and extracted by adding water, anhydrous or hydrous lower alcohols of 1 to 4 carbon atoms, ethyl acetate, acetone, chloroform or mixtures thereof. And recrystallization after solvent fractionation.

The content of frangenidine is preferably included in an amount of 1 × 10 ⁻⁵ to 10 parts by weight, and more preferably 1 × 10 ⁻⁵ to 5 parts by weight based on 100 parts by weight of the total composition. When the content is less than 1 × 10 ^-5 parts by weight, the collagen synthesis effect is insignificant, and when the content exceeds 10 parts by weight, it is not preferable in terms of application and stability to cosmetic preparation.

Genistein contained in the collagen synthesis promoting composition of the present invention is represented by the following formula (2).

[Formula 2]

Genistein represented by Formula 2 has an inhibitory effect on the expression of collagenase, which is a collagen degrading enzyme induced by ultraviolet light, and is safe for a living body, and is suitable for use as a collagen synthesis promoter. In addition, the skin wrinkle improvement and wound healing effect is very excellent, such as to improve the elasticity of the skin.

Genistein is a compound mainly present in the seeds and fruits of legumes, and most genisteins are known to exist in the form of sugar-linked genistin.

The genistein may be chemically synthesized by conventional methods known in the art, or may be extracted from plants and the like. For example, zenithine is finely chopped the fruit of the fig tree, which is a legume, warm-extracted by adding water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, chloroform or a mixture thereof, and solvent fractionation. After recrystallization can be obtained.

It is preferable that it is 1 * 10 <^-5> -10 weight part with respect to 100 weight part of total compositions, and, as for the content of genistein, it is more preferable that it is 1 * 10 <^{-5> -5} weight part. When the content is less than 1 × 10 ^-5 parts by weight, the collagen synthesis effect is insignificant, and when it is 10 parts by weight or more, it is not preferable in terms of preparation and stability as a cosmetic.

In addition, the composition for promoting collagen synthesis according to the present invention preferably has a weight ratio (genistein / frangenidine) of frangenidine and genistein to 1 to 100, and more preferably 1 to 50.

If the weight ratio of frangenidine and genistein is less than 1 or more than 100, there is a fear that the increase in collagen synthesis effect is minimal.

In the composition for promoting collagen synthesis of the present invention, additives other than the active ingredient are not particularly limited, and those commonly used in the art may be used without limitation. For example, when the composition is used for medical purposes, a pharmaceutically acceptable conventional carrier may be included, and when applied to cosmetics, conventional additives commonly used in the cosmetic field may be used without limitation.

The present invention also relates to a cosmetic for inhibiting and improving skin wrinkles comprising the composition for promoting collagen synthesis. Frangenidine has a low solubility in commonly used cosmetic solvents and is therefore limited in use in cosmetics. However, when using frangenidine and genistein at the same time as in the present invention, the above problems can be solved, and as described above, it is excellent in promoting human stability and collagen synthesis and thus can be effectively applied to cosmetics for inhibiting and improving wrinkles of the skin. have.

Cosmetics according to the present invention can be prepared by a manufacturing method generally used in the cosmetic field using the composition for promoting collagen synthesis as an active ingredient, the method is not particularly limited.

In addition, the formulation of the cosmetic according to the present invention is also not particularly limited. For example, cosmetics of the present invention powder, gel, spray, ointment, cream, liquid, skin adhesive formulation, cream, foam, lotion, pack, soft water, emulsion, foundation, makeup base, essence, soap, liquid cleansing It can be prepared as ingredients, baths, sunscreen creams, sun oils or spray liquids. Additives added in each of the above formulations is not particularly limited, either of the additives generally used in the cosmetic field may be appropriately added depending on the use thereof.

Examples of such common additives include antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, flavorings, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, and preservatives. And one or more selected.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

Production Example  One: Of frangenidin  Produce

(1) using methanol Of frangenidin  extraction

1 kg of dried roots of white paper (Angelica dahurica or Angelica dahurica var. Formosana) were placed in 10 liters of methanol, and extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 85 g of methanol extract. The hexane fraction was removed from the methanol extract through a solvent fraction, and the obtained fraction was fractionated three times with chloroform to obtain 9 g of chloroform fraction. The obtained chloroform fraction was subjected to silica column chromatography several times to obtain 0.3 g of a fraction containing franginidine, and the fraction was collected by preparative HPLC (Prep-HPLC) and recrystallization. Frangenidine was obtained from. The components and contents (99.7% by weight) were determined through nuclear magnetic resonance (NMR) and mass spectroscopy for the frangenidine obtained by the above method. 1 and 2 show the ¹ H-NMR spectrum and ¹³ C-NMR spectrum of the frangenidine, respectively, and Figure 3 shows the mass spectrum of the frangenidine.

(2) using chloroform Franginidin  extraction

1 kg of dried root of white paper was placed in 10 liters of chloroform, and extracted by heating at 100 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 12 g of chloroform extract. The chloroform extract was dissolved in chloroform, and the solvent was fractionated with an alkaline aqueous solution (0.1 M NaOH aqueous solution) to obtain an alkaline aqueous solution soluble portion, and neutralized with HCl. Subsequently, 1 g of chloroform fraction obtained by solvent fractionation with chloroform was subjected to preparative HPLC (Prep-HPLC) and recrystallization to obtain frangenidine. The components and contents (99.7% by weight) were determined through nuclear magnetic resonance (NMR) and mass spectroscopy for the frangenidine obtained by the above method.

(3) using methanol Imperatorin ( imperatorin ), Of cnidirin  Extraction and Chemical Variation

1 kg of dried root of white paper was placed in 10 liters of methanol, and extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 85 g of methanol extract. The methanol extract was partitioned three times with chloroform to yield 11 g of chloroform fraction. Subsequently, the chloroform fraction was treated with silica column chromatography to obtain 7 g of a fraction containing imperatorin and knidyrin, which are the main components of the white paper. 25 g of N, N-Diethylanilin was added to this fraction, and then subjected to a Craisen rearrangement reaction heated at 220 ° C. for 1 hour, washed with 5N hydrochloric acid solution, and then dissolved in chloroform and refrigerated. Precipitation is prepared by standing. The obtained precipitate was subjected to preparative HPLC (Prep-HPLC) and recrystallization to obtain frangenidine. Frangenidine obtained by the above method was identified by NMR and mass spectroscopy to determine the composition and content (99.7% by weight).

Production Example  2: Genistein  extraction

(1) using methanol Genistein  extraction

1 kg of dried fruit powder of Sophora japonica was placed in 10 liters of methanol, and extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain 92 g of methanol extract. The hexane fraction was removed from the methanol extract through a solvent fraction, and the obtained fraction was fractionated three times with butanol to obtain 16 g of butanol fraction. The obtained butanol fraction was subjected to silica column chromatography several times to obtain a fraction of 0.2 g containing genistein, which was subjected to preparative HPLC (Prep-HPLC) and recrystallization to obtain genistein. Genistein obtained in the above method was identified by the nuclear magnetic resonance (NMR) and mass spectroscopy (Mass spectroscopy) to determine the composition and content (99.7% by weight). 4 and 5 show the ¹ H-NMR spectrum and the mass spectrum of the genistein, respectively.

(2) treated with enzyme Genistein  extraction

1 kg of dried fruit powder of Sophora japonica was placed in 10 liters of distilled water, and extracted by heating at 90 ° C. for 3 hours in an extractor equipped with a reflux condenser to obtain a water extract. After cooling to room temperature, enzyme (β-glucosidase) was added and left for 16 hours at 40 ° C. and filtered. Subsequently, ethanol was added 10 times to the precipitate and dissolved in warming at 50 ° C. The ethanol solution was concentrated under reduced pressure and subjected to silica column chromatography several times to obtain 0.5 g of a fraction containing Genistein, which was subjected to preparative HPLC (Prep-HPLC) and recrystallization to obtain Genistein. . Genistein obtained in the above method was identified by the nuclear magnetic resonance (NMR) and mass spectroscopy (Mass spectroscopy) to determine the composition and content (99.7% by weight).

Test Example  1: Collagen Synthesis Effect Test

Frangenidine, Genistein and mixtures thereof were added to the culture of human-derived fibroblasts to test collagen synthesis effects at the cellular level. Test Example The Frangenidine and Genistein used were those prepared in Preparation Example 1 (3) and Preparation Example 2 (2). The measurement of the synthesized collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

Frangenidine was set to a final concentration of 0.1 ㎍ / ml, 0.5 ㎍ / ml, genistein was 5 ㎍ / ml, 10 ㎍ / ml, and the mixing ratio of the sample mixed with frangenidine and genistein is shown in Table 1 It was like In each evaluation, the mixture was evaluated in a concentration range where the effect was insignificant. Each sample was added to the culture medium of human fibroblast cells and cultured for 1 day, and then the culture medium was taken, and the collagen synthesis degree was measured at 450 nm by using a spectrophotometer with PICP EIA kit. .

To compare the effects, the collagen synthesis was measured by the same method for the culture medium (control) of the fibroblasts to which nothing was added and the sample to which the vitamin C was added so as to have a final concentration of 52.8 μg / ml.

Collagen production amount was measured as UV absorbance, collagen production increase rate was calculated as the ratio of the collagen production relative to the control group and the results are summarized in Table 1 below.

Sample (μg / ml) Collagen production amount (Abs) % Increase Franginidin Genistein - - 1240 - Vitamin C 52.8 µg / ml 1566 26.3 0.1 - 1278 3.1 0.5 - 1393 12.3 - 5 1416 14.2 0.1 5 1613 30.1 0.1 10 1676 35.2 0.5 5 1649 33.0 0.5 10 1700 37.1                                                            (Number of repetitions = 6)

As can be seen in the results of Table 1, when the frangenidine and genistein were mixed, it showed a better collagen synthesis effect at a lower concentration than when applying the vitamin C generally known to have collagen synthesis ability. In addition, it was found that excellent collagen synthesis effects can be obtained when frangenidine and genistein are added at the same time as compared with the case where frangenidine and genistein are added alone.

Test Example  2: Collagenase  Expression inhibitory effect

Genistein produced in (2) of Preparation Example 2 was added to the culture solution of human-derived fibroblasts at concentrations of 10 µg / ml, 20 µg / ml, and 40 µg / ml to increase the collagenase expression induced by ultraviolet rays. Inhibitory effect on the cells was tested. The measurement of collagenase was quantified using the BioTrak MMP-1 Assay Kit (Amersham Bioscience), and substituted into Equation 1 below to obtain the inhibition rate (%). The results are shown in Table 2 below. The ultraviolet control group is a group irradiated with only ultraviolet rays without processing a sample. The negative control group is a group that does not apply the sample and ultraviolet light.

[Equation 1]

Inhibition rate = (ultraviolet control-experimental group) / ultraviolet control X 100

Genistein (µg / ml) Collagenase Expression Inhibition (A450) % Inhibition 10 0.711 25 20 0.559 41 40 0.531 44 Negative Control 0.537 - UV control 0.948 -                                                             (Number of repetitions = 3)

As shown in Table 2, from the test results, it can be seen that Genistein is excellent in inhibiting the expression of collagenase.

Test Example  3: test of skin wrinkle improvement effect on mouse

A 6-week-old hairless mouse was used to test the effect of the skin wrinkles on franginidine, genistein and these mixed samples against skin wrinkles induced by light. Frangenidine and Genistein were prepared by dissolving 5 mg / ml of 1,3-butylene glycol prepared in Preparation Example 1 (3) and Preparation Example 2 (2), respectively. 1 mg / ml, 4 mg / ml of Genistein was prepared and applied.

The hairless mouse was irradiated with 2 MED for 3 days a week for 10 weeks using a solar simulator to form skin wrinkles, and the control treated with 1,3-butylene glycol without addition of the sample and 5 The degree of improvement was qualitatively determined in the group treated with 0.5 ml / cm 2 (2.5 mg / cm 2 as sample) twice a day for 6 weeks.

Wrinkle improvement was judged by visual inspection and photographing of the sample treatment area first, and the judgment criteria were judged by three stages of no improvement, slight improvement, and significant improvement compared to the sample treatment group and the control group. It is shown in Table 3 below.

No improvement Minor improvements A significant improvement Control 9 One 0 Franginidin 3 4 3 Genistein 4 5 One Mixed Sample 0 5 5                                                      (Population in each group = 10)

As shown in Table 3, it can be seen that when a mixture of frangenidine and genistein is applied, there is an excellent wrinkle improvement effect than that of pragenidine or genistein, respectively.

Example  1 and Comparative example  1 to 3

A cosmetic essence was prepared using the general method in the field of cosmetic production, the composition shown in Table 4 below, the balance of which is adjusted to 100 parts by weight of the total weight as purified water.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Franginidin 0.1 - 0.5 - Genistein 0.4 - - 0.5 Propylene glycol 10.0 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 10.0 Sodium hyaluronate (1%) 5.0 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 0.1 incense 0.05 0.05 0.05 0.05                                                               (Unit: parts by weight)

Test Example  4: Cosmetics Essence  Wrinkle improvement effect test

Through the panel test, the skin wrinkle improvement effect of the cosmetic essences of Example 1 and Comparative Examples 1 to 3 was tested. Specifically, 60 healthy women from 35 to 50 years old were divided into four groups of 15 people, group 1 of the essence of Comparative Example 1, group 2 of the essence of Comparative Example 2, group 3 of the Comparative Example 3 The essence was applied to the group 4, and the essence of Example 1 was applied to the face part once a day for 3 months. Three months later, the extent of skin wrinkle improvement was assessed through a survey.

The subject's questionnaire was judged as three stages of improvement, slight improvement, and significant improvement compared to before use regarding skin wrinkle improvement and elasticity enhancement. The results are shown in Table 5 below.

No improvement Minor improvements A significant improvement Comparative Example 1 9 4 2 Comparative Example 2 2 9 4 Comparative Example 3 4 7 4 Example 1 - 6 9

As can be seen in Table 5, when using the essence of Example 1 containing frangenidine and genistein, each of them alone or using the essence of Comparative Examples 1 to 3 without using the skin wrinkle improvement effect It can be seen that excellent.

Test Example  5: through image analysis Cosmetics Essence  Wrinkle improvement effect test

The wrinkle improvement effect of Example 1 and Comparative Examples 1 to 3 was evaluated through image analysis. Specifically, before the experiment begins, replicas under the eyes (Xantopren, Bayer) are taken. After the experiments are completed, replicas are taken from the same area under the eyes. The density of skin wrinkles was measured. The reduction rate of skin wrinkle density by image analysis is a value obtained by averaging the ratio of skin wrinkle density after use to skin wrinkle density before use of essence, and the measurement results are summarized in Table 6 below.

Skin wrinkle density reduction rate (%) Comparative Example 1 4 Comparative Example 2 35 Comparative Example 3 24 Example 1 48

As can be seen in Table 6, when using the essence of Example 1 containing frangenidine and genistein, each of them alone or using the essence of Comparative Examples 1 to 3 that do not use the skin wrinkle improvement effect It can be seen that excellent. In addition, no skin irritation or side effects due to the essence of Example 1 was found in the test procedure.

Example  2

Through each of the compositions shown below, various cosmetic formulations were prepared.

1) Skin ointment (unit: parts by weight)

Franginidin 0.2 Genistein 0.8 Diethyl sebacate 8 Prepayment 5 Polyoxyethylene Oleyl Ether Phosphate 6 Sodium benzoate 0.1 Adjust the total weight to 100 by using Waselin

2) Cosmetic cream (unit: parts by weight)

Franginidin 0.04 Genistein 0.16 Stearic acid 15.0 Cetanol 1.0 Potassium hydroxide 0.7 glycerin 5.0 Propylene glycol 3.0 antiseptic 0.05 incense 0.05 Adjust the total weight to 100 by the amount of purified water remaining

3) Flexible cosmetics (unit: parts by weight)

Franginidin 0.04 Genistein 0.16 ethanol 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 Methyl paraoxybenzoate 0.2 glycerin 5.0 1,3-butylene glycol 6.0 incense 0.05 Pigment 0.05 Adjust the total weight to 100 by the amount of purified water remaining

4) Nutrients (unit: parts by weight)

Franginidin 0.02 Genistein 0.08 Waselin 2.0 Sesquioleate sorbitan 0.8 Polyoxyethylene oleylethyl 1.2 Methyl paraoxybenzoate 0.1 Propylene glycol 5.0 ethanol 3.2 Carboxy Vinyl Polymer 18.0 Potassium hydroxide 0.1 Pigment 0.05 incense 0.05 Adjust the total weight to 100 by the amount of purified water remaining

5) Cosmetic Pack (Unit: Weight part)

Franginidin 0.04 Genistein 0.16 glycerin 5.0 Propylene glycol 4.0 Polyvinyl alcohol 15.0 ethanol 8.0 Polyoxyethylene oleylethyl 1.0 Methyl paraoxybenzoate 0.2 Pigment 0.05 incense 0.05 Adjust the total weight to 100 by the amount of purified water remaining

6) Men's Skin (Unit: Weight part)

 Franginidin 0.02 Genistein 0.08 ethanol 55.0 PEG-40 Hydrogenated castor oil 0.5 1,3-butylene glycol 1.0 Glycereth-26 1.0 antiseptic 0.05 incense 0.05 Adjust the total weight to 100 by the amount of purified water remaining

As described above, the composition for promoting collagen synthesis including frangenidine and genistein shows a very strong collagen synthesis effect on fibroblasts of the skin, and the cosmetics including the same have excellent wrinkle improvement.

The synergistic effect of the combined application of frangenidine and genistein is thought to show a superior effect by using together genistein which is relatively excellent in inhibiting the expression of frangenidine and collagenase, which have relatively superior collagen biosynthesis. This means that the lower solubility of frangenidine in general cosmetic formulations may be used at lower concentrations, and thus may be applicable to products of various formulations.

1 is a ¹ H-NMR spectrum graph of the frangenidine prepared in Preparation Example 1 of the present invention.

2 is a ¹³ C-NMR spectrum graph of the frangenidine prepared in Preparation Example 1 of the present invention.

3 is a mass spectrum graph of the frangenidine prepared in Preparation Example 1 of the present invention.

4 is a ¹ H-NMR spectral graph of Genistein prepared in Preparation Example 2 of the present invention.

5 is a mass spectrum graph of genistein prepared in Preparation Example 2 of the present invention.

Claims (10)

A composition for promoting collagen synthesis, comprising a compound of Formula 1 and a compound of Formula 2: [Formula 1] [Formula 2]

,

. The method of claim 1, Compound of Formula 1 is a composition for promoting collagen synthesis, characterized in that contained in an amount of 1 × 10 ^-5 to 10 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 2, Compound of Formula 1 is a composition for promoting collagen synthesis, characterized in that contained in an amount of 1 × 10 ^-5 to 5 parts by weight with respect to 100 parts by weight of the total composition. The method of claim 1, The compound of formula (2) is a composition for promoting collagen synthesis, characterized in that contained in an amount of 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition. The method of claim 4, wherein The compound of formula (2) is a composition for promoting collagen synthesis, characterized in that contained in an amount of 1 × 10 ^-5 to 5 parts by weight based on 100 parts by weight of the total composition. The method of claim 1, A composition for promoting collagen synthesis, characterized in that the weight ratio of the compound of Formula 1 and the compound of Formula 2 (compound of Formula 2 / compound of Formula 1) is 1 to 100. The method of claim 6, A composition for promoting collagen synthesis, characterized in that the weight ratio of the compound of Formula 1 and the compound of Formula 2 (compound of Formula 2 / compound of Formula 1) is 1 to 50. A cosmetic for inhibiting and improving skin wrinkles, comprising the composition for promoting collagen synthesis according to any one of claims 1 to 7.  The method of claim 8, Formulations are powders, gels, sprays, ointments, creams, liquids, skin adhesive formulations, creams, foams, lotions, packs, softeners, emulsions, foundations, makeup bases, essences, soaps, liquid cleansers, baths, sunscreens Cosmetics for inhibiting and improving skin wrinkles, characterized in that the cream, sun oil or spray liquid. The method of claim 9, One or more selected from the group consisting of antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, and preservatives Cosmetics for inhibiting and improving skin wrinkles.

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