KR101418986B1 - Composition for promoting collagen synthesis and cosmetics comprising the same - Google Patents

Abstract

The present invention relates to a composition for promoting collagen synthesis comprising flangenidine and genistein represented by a specific formula. The composition of the present invention has excellent collagen formation promoting ability and collagenase inhibiting ability, and even when the effective ingredient is contained in a small amount, it has an advantage that it exhibits excellent efficacy. Accordingly, the composition of the present invention can be utilized in various formulations, and cosmetics containing the same have excellent wrinkle suppressing and improving effects.

Promenidine, Genistein, promoting collagen synthesis, suppressing wrinkles, improving wrinkles, cosmetics

Description

[0001] The present invention relates to a composition for promoting collagen synthesis and a cosmetic composition containing the same,

The present invention relates to a composition for promoting collagen synthesis and a cosmetic for suppressing and improving wrinkles containing the same, more specifically, to a composition for promoting collagen synthesis, which comprises progenidin and genistein, And to an improvement cosmetic product.

Collagen is a major constituent of the extracellular matrix and is a major substrate protein produced by the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). Collagen also exists as fibrous inclusion bodies in most other structures.

Collagen is a relatively weak immunogen, which is caused by the occlusion of latent antigenic determinants by the helical structure, and this helical structure also causes the collagen to be resistant to proteolysis. The main functions of collagen are known to be mechanical rigidity of skin, resistance of connective tissue, binding force of tissue, support of cell adhesion, and induction of cell division and differentiation (when organism grows or heals the wound) (Van der Rest et al. , Ann NY Acad Sci, 1990). Such collagen is reduced by aging and photoaging by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in healing wounds and can accelerate the synthesis of collagen in the injured epithelium, allowing the wounds to quickly recover without scarring.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it does not exhibit essential skin function improvement and wound healing function.

As a result, there is a growing interest in collagen synthesis promoting substances. Vitamin C, retinoic acid, and TGF (transforming growth factor, Cardinale G. et al., Adv. Enzymol., 41, p. 425, 1974).

Japanese Patent Application Laid-Open No. 8-231370 discloses a protein derived from an animal placenta as collagen synthesis material, and Japanese Patent Laid-Open Publication No. 8-208424 discloses betulinic acid, 9- 40523 and 10-36283 disclose chlorella extracts and the like which exhibit fibroblast proliferation promoting action.

However, the above-mentioned conventional materials are limited in the amount of use due to safety problems such as irritation and redness at the time of applying the skin, and the effect is insignificant, so that the effect of skin function improvement or wound healing can not be expected substantially. Therefore, there is a desperate need to develop a novel composition for external skin application that is safer and more effective in living body than existing skin external composition compositions.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a composition for promoting collagen synthesis, which exhibits excellent collagen synthesis promoting effect with excellent stability to a living body and excellent workability such as solubility in a solvent, .

Accordingly, an object of the present invention is to provide a composition for promoting collagen synthesis, which comprises francidinine and genistein represented by a specific formula.

It is another object of the present invention to provide cosmetic compositions of various formulations including the composition for promoting collagen synthesis.

The present invention provides, as means for solving the above-mentioned problems, a composition for promoting collagen synthesis comprising a compound of the following formula (1) and a compound of the following formula (2).

[Chemical Formula 1] < EMI ID =

The content of frangenidine represented by the formula (1) contained in the composition of the present invention is preferably 1 × 10 ^-5 to 10 parts by weight per 100 parts by weight of the total composition.

The content of the genistein represented by the general formula (2) contained in the composition of the present invention is preferably 1 × 10 ^-5 to 10 parts by weight per 100 parts by weight of the total composition.

Another object of the present invention is to provide a cosmetic composition for suppressing and improving skin wrinkles comprising the composition for promoting collagen synthesis.

The composition for promoting collagen synthesis of the present invention is excellent in stability to living body, exhibits excellent collagen formation promoting effect, and can be applied variously to cosmetics and medicinal uses. In particular, the composition of the present invention is applied to cosmetics and exhibits excellent effects on wrinkle suppression and improvement.

The present invention relates to a composition for promoting collagen synthesis comprising flangenidine and genistein represented by a specific formula. The composition of the present invention has excellent collagen formation promoting ability and collagenase inhibiting ability, and even when the effective ingredient is contained in a small amount, it has an advantage that it exhibits excellent efficacy.

Hereinafter, the composition for promoting collagen synthesis of the present invention will be described in detail as follows.

The flangenein contained in the composition for promoting collagen synthesis according to the present invention is represented by the following formula (1).

[Chemical Formula 1]

Since francenidine represented by the above formula (1) is safe for living body and has an effect of promoting collagen synthesis of skin fibroblasts, it is suitable to be used as a collagen synthesis promoter, and can improve the elasticity of skin, The healing effect is very good.

Frangenidine is a compound mainly present in the roots of a mountainous tree and a plant, and can be chemically synthesized by a conventional method known in the art, or extracted from plants and the like. For example, frangenidine is prepared by finely dividing the roots (white paper) of the phyllotactic tree and plant, and adding water, anhydrous or lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, chloroform or a mixture thereof, Followed by solvent fractionation and recrystallization.

The content of flangenidine is preferably 1 x 10 ^-5 to 10 parts by weight, more preferably 1 x 10 ^-5 to 5 parts by weight, based on 100 parts by weight of the total composition. When the content is less than 1 占^0-5 parts by weight, the effect of synthesizing collagen is insignificant, and when it exceeds 10 parts by weight, it is not preferable from the standpoint of application to cosmetics and stability.

The genistein contained in the composition for promoting collagen synthesis of the present invention is represented by the following formula (2).

(2)

The genistein represented by Formula 2 has an inhibitory effect on the increase of collagenase expression, which is a collagenase caused by ultraviolet rays, and is safe for living body, and is suitable for use as collagen synthesis promoter. In addition, the skin wrinkle improving effect and the wound healing effect such as improving the elasticity of the skin are excellent.

Genistein is a compound mainly present in the seeds and fruit of leguminous plants, and most genistein is known to exist in the form of sugar-linked genistein.

The genistein can be chemically synthesized by a conventional method known in the art or extracted from plants and the like. For example, genistein is obtained by cutting fruit of a flower tree, which is a soybean plant, by heating, adding water, an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, chloroform or a mixture thereof, Followed by recrystallization.

The content of the genistein is preferably 1 × 10 ^-5 to 10 parts by weight, more preferably 1 × 10 ^-5 to 5 parts by weight, based on 100 parts by weight of the whole composition. If the content is less than 1 x ^10-5 parts by weight, the effect of synthesizing collagen is insignificant, and if it is more than 10 parts by weight, it is not preferable from the standpoint of preparation as a cosmetic preparation and stability.

In addition, the composition for promoting collagen synthesis according to the present invention preferably has a weight ratio (genistein / flananidine) of flananidin and genistein of 1 to 100, more preferably 1 to 50.

If the weight ratio of flangeneidin and genistein is less than 1 or more than 100, the effect of collagen synthesis may be insignificantly increased.

In the composition for promoting collagen synthesis of the present invention, additives other than the above-mentioned effective ingredient are not particularly limited, and those commonly used in this field can be used without limitation. For example, when the composition is used for medical purposes, a pharmaceutically acceptable carrier may be included. When it is applied to cosmetics and the like, conventional additives commonly used in the cosmetics field may be used without limitation.

The present invention also relates to a cosmetic for suppressing and improving skin wrinkles containing the composition for promoting collagen synthesis. Francogenidin has low solubility in commonly used cosmetic solvents and its use in cosmetics is limited. However, as described in the present invention, the use of both flangenidine and genistein at the same time can solve the above-mentioned problems. Further, as described above, the present invention can be effectively applied to cosmetics for suppressing and improving wrinkles of skin, have.

The cosmetics according to the present invention can be produced by a method generally used in the field of cosmetics, using a composition for promoting collagen synthesis as an active ingredient, and the method is not particularly limited.

The formulation of the cosmetic according to the present invention is also not particularly limited. For example, the cosmetic of the present invention may be in the form of a powder, a gel, a spray, an ointment, a cream, a liquid, a skin adhesive type formulation, a cream, a foam, a lotion, a pack, a soft water, a milky lotion, a foundation, a makeup base, A bath liquid, a sunscreen cream, a sun oil, or a spray liquid. The additives to be added in each of the formulations are also not particularly limited, and the additives commonly used in the cosmetics field can be appropriately added according to the purpose of use.

Examples of such common additives include those from the group consisting of antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, One or more selected may be mentioned.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Manufacturing example  One: Frangidin  Produce

(1) Using methanol Frangidin  extraction

1 kg of dried root of Angelica dahurica or Angelica dahurica var. Formosana was placed in 10 liters of methanol and heated at 80 ° C for 3 hours in an extractor equipped with a reflux condenser to obtain 85 g of methanol extract. The hexane fraction was removed from the methanol extract through the solvent fraction, and the obtained fraction was fractionated three times with chloroform to obtain 9 g of a chloroform fraction. The obtained chloroform fraction was treated with silica column chromatography several times to obtain 0.3 g of a fraction containing flangenidine. The fractions were fractionated by preparative HPLC (Prep-HPLC) and recrystallization method, ≪ / RTI > The content and content (99.7% by weight) of frangenidine obtained by the above method were confirmed by nuclear magnetic resonance (NMR) and mass spectroscopy. 1 and 2 show the ¹ H-NMR spectrum and ¹³ C-NMR spectrum of the flanganine, respectively, and Fig. 3 shows the mass spectrum of the flanganine.

(2) Using chloroform Franginidin  extraction

1 kg of dried white roots was added to 10 liters of chloroform and heated at 100 ° C for 3 hours with an extractor equipped with a reflux condenser to obtain 12 g of chloroform extract. The chloroform-extract solution was dissolved in chloroform and solvent fractionated with an aqueous alkaline solution (0.1 M NaOH aqueous solution) to obtain an aqueous alkali solution-soluble fraction, which was then neutralized with HCl. Subsequently, preparative HPLC (prep-HPLC) and recrystallization treatment were carried out on 1 g of the chloroform fraction obtained by solvent fractionation with chloroform to obtain flangendin. The content and content (99.7% by weight) of frangenidine obtained by the above method were confirmed by nuclear magnetic resonance (NMR) and mass spectroscopy.

(3) Using methanol Imperatrone ( imperatorin ), Cnidirin  Extraction and chemical transformation

1 kg of dried white roots was placed in 10 liters of methanol and heated at 80 DEG C for 3 hours with an extractor equipped with a reflux condenser to obtain 85 g of a methanol extract. The methanol extract was fractionated three times with chloroform to obtain 11 g of a chloroform fraction. Subsequently, the chloroform fraction was treated with silica column chromatography to obtain 7 g of a fraction containing impairatrin and chinidine as main components of white paper. To this fraction, 25 g of N, N-diethylanilin was added and the mixture was rearranged by heating at 220 ° C for 1 hour. The reaction mixture was washed with 5N hydrochloric acid solution and then dissolved in chloroform The precipitate is produced by allowing to stand. The resulting precipitate was subjected to preparative HPLC (Prep-HPLC) and recrystallization method to obtain flangendin. The content and content (99.7% by weight) of frangenidine obtained by the above method were confirmed by NMR and mass spectroscopy.

Manufacturing example  2: Genistein  extraction

(1) Using methanol Genistein  extraction

1 kg of dried fruit powder of Sophora japonica was placed in 10 liters of methanol and heated at 80 ° C for 3 hours in an extractor equipped with a reflux condenser to obtain 92 g of a methanol extract. The hexane fraction was removed from the methanol extract through a solvent fraction, and the obtained fraction was fractionated three times with butanol to obtain 16 g of a butanol fraction. The obtained butanol fraction was subjected to silica column chromatography several times over several times to obtain 0.2 g of a fraction containing genistein. Preparative HPLC (Prep-HPLC) and recrystallization were performed on this fraction to obtain genistein. The content and the content (99.7% by weight) of the genistein obtained by the above method were confirmed by nuclear magnetic resonance (NMR) and mass spectroscopy. 4 and 5 show the ¹ H-NMR spectrum and the mass spectrum of the above-mentioned genistein, respectively.

(2) enzyme-treated Genistein  extraction

1 kg of dried fruit powder of Sophora japonica was placed in 10 liters of distilled water and heated at 90 ° C for 3 hours in an extractor equipped with a reflux condenser to obtain a water extract. After cooling at room temperature, the enzyme (β-glucosidase) was added and allowed to stand at 40 ° C. for 16 hours and then filtered. Then, 10 times of ethanol was added to the precipitate and dissolved by heating at 50 占 폚. The ethanol solution was concentrated under reduced pressure and subjected to silica column chromatography on several occasions to obtain 0.5 g of a fraction containing genistein. Preparative HPLC (Prep-HPLC) and recrystallization were performed on this fraction to obtain genistein . The content and the content (99.7% by weight) of the genistein obtained by the above method were confirmed by nuclear magnetic resonance (NMR) and mass spectroscopy.

Test Example  1: Collagen synthesis effect test

The effects of collagen synthesis at the cellular level were tested by adding flangenidine, genistein, and mixtures thereof to the culture medium of human-derived fibroblasts. Test Example The frangidine and genistein used were those prepared in (3) of Production Example 1 and (2) of Production Example 2. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

The mixture ratio of the sample in which flangenidine and genistein were mixed was as shown in the following Table 1: < tb > < TABLE > Id = TABLE 1 Columns = 2 < tb > Respectively. In each evaluation, the effects were evaluated by mixing in a concentration range that was insignificant. Each sample was added to the culture medium of human fibroblast cells for 1 day, and the culture was taken. The degree of collagen synthesis was measured at 450 nm using a PICP EIA kit using a spectrophotometer at each concentration .

For the comparison of the effects, the degree of collagen synthesis was measured by the same method for the culture medium of fibroblasts (control group) to which nothing had been added and the sample added with vitamin C to a final concentration of 52.8 / / ml.

The amount of collagen production was measured as the UV absorbance, and the increase rate of collagen production was calculated as a ratio of the relative amount of collagen production to the control. The results are summarized in Table 1 below.

Samples (/ / ml) Collagen production (Abs) Growth rate (%) Franginidin Genistein - - 1240 - Vitamin C 52.8 μg / ml 1566 26.3 0.1 - 1278 3.1 0.5 - 1393 12.3 - 5 1416 14.2 0.1 5 1613 30.1 0.1 10 1676 35.2 0.5 5 1649 33.0 0.5 10 1700 37.1                                                            (Number of repetitions = 6)

As can be seen from the results shown in Table 1, when francidin and genistein were mixed, the collagen synthesis effect was better than that of vitamin C, which is generally known to have collagen synthesis ability. In addition, it was found that, when both flangenide and genistein were simultaneously added, an excellent collagen synthesis effect could be obtained, as compared with the case where each of flangenidine and genistein was added singly.

Test Example  2: Collagenase  Expression inhibitory effect

Genistein produced in Production Example 2 (2) was added to the culture medium of human-derived fibroblasts at a concentration of 10 占 퐂 / ml, 20 占 퐂 / ml, and 40 占 퐂 / ml to increase the expression of collagenase induced by ultraviolet The inhibitory effect was tested. Measurement of collagenase was quantitated using a BioTrak MMP-1 assay kit (Amersham Bioscience), and the inhibition rate (%) was determined by substituting in the following equation (1). The results are shown in Table 2 below. The ultraviolet control group was irradiated only with ultraviolet light without treating the sample. The negative control group is a sample and a group not irradiated with ultraviolet rays.

[Formula 1]

Inhibition rate = (ultraviolet control - test group) / ultraviolet control X 100

Genistein (占 퐂 / ml) Collagenase expression inhibition (A450) % Inhibition 10 0.711 25 20 0.559 41 40 0.531 44 Negative control group 0.537 - Ultraviolet control 0.948 -                                                             (Number of repetitions = 3)

As shown in Table 2, from the test results, it was found that the effect of inhibiting cholinergic expression of genistein was excellent.

Test Example  3: Test for improving skin wrinkles on mice

Using 6-week-old hairless mice, the effect of improving the skin wrinkles of frangenidin, genistein, and the mixed samples thereof was tested against light-induced skin wrinkles. Frannidin and genistein were dissolved in 1,3-butylene glycol (5 mg / ml) prepared in Preparation Example 1 (3) and Preparation Example 2 (2), respectively, 1 mg / ml, and genistein 4 mg / ml.

Hairless mice were irradiated with 2 MED for 3 weeks for 10 weeks using a solar simulator to form skin wrinkles. A control group treated with 1,3-butylene glycol without a sample and 5 mg / ml of the sample solution was treated with 0.5 ml / ㎠ (2.5 mg / ㎠ as a sample) twice a day for 6 weeks.

The degree of wrinkle improvement was judged by naked eyes through visual observation and photographing, and the judgment criteria were three stages of no improvement, little improvement, and considerable improvement compared with those before the sample treatment in the sample treatment group and the control group Table 3 shows the results.

No improvement Slight improvement A significant improvement Control group 9 One 0 Franginidin 3 4 3 Genistein 4 5 One Mixed sample 0 5 5                                                      (The number of each group = 10)

As shown in Table 3 above, it can be seen that when mixed with francidinin and genistein, the wrinkle-improving effect is better than when pragogenin or genistein is applied.

Example  1 and Comparative Example  1 to 3

Using the general method in the field of cosmetics manufacturing, cosmetic essences containing the composition shown in the following Table 4 and having the remaining amount adjusted to 100 parts by weight as the purified water were prepared.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Franginidin 0.1 - 0.5 - Genistein 0.4 - - 0.5 Propylene glycol 10.0 10.0 10.0 10.0 glycerin 10.0 10.0 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 0.1 0.1 incense 0.05 0.05 0.05 0.05                                                               (Unit: parts by weight)

Test Example  4: Cosmetics Essence  Wrinkle improvement test

The skin wrinkle improving effect of the cosmetic essence of Example 1 and Comparative Examples 1 to 3 was tested through a panel test. More specifically, 60 healthy women aged 35 to 50 years were divided into four groups of 15 individuals. Group 1 was the essence of Comparative Example 1, Group 2 was Essence of Comparative Example 2, Group 3 was Comparative Example 3 The essence of Example 1 was applied to the face of the fourth group for once a day for three months. After three months, the improvement of skin wrinkles was assessed by questionnaires.

The subjects' questionnaires were judged to have no improvement, little improvement, and considerable improvement compared to before use with regard to skin wrinkle improvement and elasticity enhancement, and the results are shown in Table 5 below.

No improvement Slight improvement A significant improvement Comparative Example 1 9 4 2 Comparative Example 2 2 9 4 Comparative Example 3 4 7 4 Example 1 - 6 9

As can be seen from the above Table 5, in the case of using essences of Example 1 including flangenidine and genistein, the effect of improving the wrinkles of the skin was higher than that of the essences of Comparative Examples 1 to 3, It can be seen that it is excellent.

Test Example  5: Through image analysis Cosmetics Essence  Wrinkle improvement test

The wrinkle-reducing effect of Example 1 and Comparative Examples 1 to 3 was evaluated through image analysis. Before the experiment, the replica under the eyes was taken (Xantopren, Bayer), and the replica was taken from the same area under the eye immediately after the end of the experiment and analyzed by two-dimensional analysis of the skin wrinkles The density of skin wrinkles was measured. The reduction rate of skin wrinkle density by image analysis is a value obtained by averaging the ratio of skin wrinkle density after use to skin wrinkle density before use of Essence.

Skin Wrinkle Density Reduction (%) Comparative Example 1 4 Comparative Example 2 35 Comparative Example 3 24 Example 1 48

As can be seen from the above Table 6, when essences of Example 1 including flangenidine and genistein were used, the effect of improving the wrinkles of the skin was improved compared with the case of using Essence of Comparative Examples 1 to 3, It can be seen that it is excellent. In addition, no skin irritation or side effects due to essence of Example 1 were found in the above test procedure.

Example  2

Through various compositions shown below, various cosmetic formulations were prepared.

1) ointment for external skin (unit: parts by weight)

Franginidin 0.2 Genistein 0.8 Diethyl sebacate 8 Nonsense 5 Polyoxyethylene oleyl ether phosphate 6 Sodium benzoate 0.1 Vaseline was adjusted to 100 parts by weight as the balance

2) Cosmetic cream (unit: parts by weight)

Franginidin 0.04 Genistein 0.16 Stearic acid 15.0 Cetanol 1.0 Potassium hydroxide 0.7 glycerin 5.0 Propylene glycol 3.0 antiseptic 0.05 incense 0.05 Purified water is used as the balance to adjust the total weight part to 100

3) Longevity (unit: parts by weight)

Franginidin 0.04 Genistein 0.16 ethanol 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 Methyl paraoxybenzoate 0.2 glycerin 5.0 1,3-butylene glycol 6.0 incense 0.05 Pigment 0.05 Purified water is used as the balance to adjust the total weight part to 100

4) Nutritional lotion (unit: parts by weight)

Franginidin 0.02 Genistein 0.08 Vaseline 2.0 Sesquioleic acid sorbitan 0.8 Polyoxyethylene oleylethyl 1.2 Methyl paraoxybenzoate 0.1 Propylene glycol 5.0 ethanol 3.2 Carboxyvinyl polymer 18.0 Potassium hydroxide 0.1 Pigment 0.05 incense 0.05 Purified water is used as the balance to adjust the total weight part to 100

5) Cosmetic pack (unit: parts by weight)

Franginidin 0.04 Genistein 0.16 glycerin 5.0 Propylene glycol 4.0 Polyvinyl alcohol 15.0 ethanol 8.0 Polyoxyethylene oleylethyl 1.0 Methyl paraoxybenzoate 0.2 Pigment 0.05 incense 0.05 Purified water is used as the balance to adjust the total weight part to 100

6) Men's skin (unit: parts by weight)

 Franginidin 0.02 Genistein 0.08 ethanol 55.0 PEG-40 Hydrogenated castor oil 0.5 1,3-butylene glycol 1.0 Glycereth-26 1.0 antiseptic 0.05 incense 0.05 Purified water is used as the balance to adjust the total weight part to 100

As described above, the composition for promoting collagen synthesis including progenidin and genistein showed a very strong collagen synthesis effect on the fibroblasts of the skin, and cosmetics containing the collagen synthesis enhances the wrinkle-improving effect.

It is considered that the synergistic effect by the combined use of francidin and genistein is more effective by using together francidin which is excellent in collagen biosynthesis ability and genicin that is excellent in inhibition of collagenase expression. This means that it can be used in a wide variety of formulations because it exhibits effects while using less low concentration of flangenein in common cosmetic formulations.

1 is a ¹ H-NMR spectral graph of frangerin prepared in Preparation Example 1 of the present invention.

2 is a ¹³ C-NMR spectral graph of frangerin prepared in Preparation Example 1 of the present invention.

3 is a mass spectrum graph of frangerin prepared in Preparation Example 1 of the present invention.

4 is a ¹ H-NMR spectral graph of the genistein produced in Production Example 2 of the present invention.

5 is a mass spectrum graph of the genistein produced in Production Example 2 of the present invention.

Claims (10)

(1), wherein the weight ratio of the compound of the formula (1) to the compound of the formula (2) is 1 to 100, Composition: [Chemical Formula 1] < EMI ID =

,

. The method according to claim 1, Wherein the compound of formula (1) is contained in an amount of 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition. 3. The method of claim 2, Wherein the compound of formula (1) is contained in an amount of 1 × 10 ^-5 to 5 parts by weight based on 100 parts by weight of the total composition. The method according to claim 1, Wherein the compound of formula (2) is contained in an amount of 1 × 10 ^-5 to 10 parts by weight based on 100 parts by weight of the total composition. 5. The method of claim 4, Wherein the compound of formula (2) is contained in an amount of 1 × 10 ^-5 to 5 parts by weight based on 100 parts by weight of the total composition. delete The method according to claim 1, A composition for accelerating collagen synthesis, wherein the weight ratio (compound of formula (2) / compound of formula (1)) of the compound of formula (1) and the compound of formula (2) is 1 to 50. A cosmetic composition for suppressing and improving wrinkles of skin, which comprises the composition for promoting collagen synthesis according to any one of claims 1 to 5 and 7.  9. The method of claim 8, The formulation may be in the form of a powder, a gel, a spray, an ointment, a cream, a liquid, a skin adhesive type formulation, a cream, a foam, a lotion, a pack, a soft water, a milky liquid, a foundation, a makeup base, A cosmetic product for suppressing or improving skin wrinkles characterized by being a cream, a sun oil or a spray liquid. 10. The method of claim 9, At least one selected from the group consisting of antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments and preservatives A cosmetic for preventing and improving skin wrinkles.

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