KR101521240B1 - Cosmetic composition for preventing allergy and atopy containing the extract of fermentative Scutellaria baicalensis by Laetiporus Sulphureus - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, fermented solution of Scutellaria Leatiporus sulphureus var. miniatus obtained by fermenting the Scutellaria baicalensis extract with mycelium of Leatiporus sulphureus var. miniatus. The cosmetic composition containing, as an active ingredient, fermented solution of Scutellaria Leatiporus sulphureus var. miniatus has excellent effects of preventing skin aging, and improving skin wrinkles, skin moisture, skin whitening, skin barrier function, skin inflammation, and atopic dermatitis.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for preventing skin inflammation and atopic eczema containing an extract of fermented scutellaria baicalensis by Laetiporus Sulphureus,

The present invention relates to a cosmetic composition containing as an active ingredient a fermented product of a golden red mildew obtained by fermenting a golden extract with a mycelium of red mildew. More specifically, the present invention relates to a cosmetic composition containing a golden red mildew fermented liquid as an active ingredient, Skin wrinkle improvement, skin moisturizing, skin whitening, skin barrier function improvement, skin inflammation improvement and atopic improvement.

Skin aging can be divided into two types: intrinsic (chronological aging) and phtoaging (Gilchrest BA: J. Am. Acad. Dermatol ., 21, 610-613 (1989)]. Endogenous aging is a naturally occurring aging phenomenon that is accompanied by decreased physiological function of the body as age increases [Braverman IM et al . : J. Invest. Dermatol ., 78, 434-443 (1982)]. Photoaging means that the skin is repeatedly exposed to light to change the appearance or function of the skin [Ridder GM et al . : J. Am. Acad. Dermatol ., 25, 751-760 (1991)]. In addition to the above, skin aging can be caused by active oxygen species activated by ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. When such conditions are deepened, the antioxidant defense network existing in the living body is destroyed, And tissues to promote adult diseases and aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins causes severe hyperinflammation of collagen, hyaluronic acid, elastin, proteoglycans, and fibronectin, which are connective tissues of the skin, which interferes with skin elasticity. Mutation, cancer induction, and immune function.

Therefore, it is necessary to protect the cell membranes by abolishing reactive oxygen species that are mediated by the body's metabolic processes, ultraviolet irradiation, and inflammatory reactions, and regenerate already damaged cells by active metabolism to proliferate the cells Can be restored and healthy skin can be maintained. In aging, not only reactive oxygen species but also MMP (Matrix metallopretease) enzyme is involved. Synthesis and degradation of extracellular matrix such as collagen are controlled appropriately in vivo, but its synthesis is decreased as aging progresses and enzymes degrading collagen (MMP) is promoted and the elasticity of the skin is lowered and wrinkles are formed. In addition, these degrading enzymes are activated by ultraviolet exposure. Therefore, there is a demand for development of a substance capable of controlling the activation of MMP induced in the cell by ultraviolet light or inhibiting its activity. However, the raw materials used as cosmetic materials have mostly suppressed only the activity of degrading enzyme (MMP).

On the other hand, melanin is converted from tyrosine to dopa and dopaquinone by the action of tyrosinase existing in pigment cells, and is generated by dopachrome. Melanin is present in the skin, protects the body from ultraviolet light and has an important function in regulating hormone secretion in the body. However, when melanin is overproduced, it is known that it forms spots and freckles, promotes skin aging, and plays an important role in skin cancer induction, and research and development for preventing melanin overproduction has been actively carried out. (Japanese Patent Laid-open No. 4-93050) and plant extracts inhibit tyrosinase (Japanese Patent Laid-open Publication No. 4-9320), hydroquinone (Japanese Patent Laid Open No. 6-192062), kojic acid And is used as a whitening cosmetic. However, these components are poor in stability among cosmetic formulations, they are decomposed to cause coloration, odor generation, efficacy at a living body level, effects are unclear, or safety is a problem, and their use is limited.

On the other hand, in the human body, the skin is an organ acting as a protective film for protecting the living body from the external environment. It prevents loss of biological components in the human body, that is, water and electrolytes, and prevents harmful substances from entering the human body from the outside . The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer consists of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they are required to have strong resistance against physical, chemical, or material permeation and prevent moisture from being lost to the outside At the same time as zooming, it should maintain its flexibility by holding proper water on its own.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer, which is about 10 to 30% in the keratin layer. The water supplied from the dermis layer mainly migrates to the upper part of the stratum corneum by passive diffusion and eventually to the outside. This is called Trans Epidermal Water Loss (TEWL). It is known that the lipid component of the keratinocyte layer and epidermal lipid, which maintains the transdermal water loss at an appropriate level, is the lipid component.

On the other hand, it is known that the keratinocyte layer has a hydrophilic water retention ability called a natural moisturizing factor (NMF) and plays an important role in moisturizing the skin. Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost and the aging phenomenon occurs. For example, in the case of dry skin, the keratinocyte aggregation is weakened, and a scaling phenomenon appears in which the keratinocytes are peeled off like scales from the skin surface. The reason why the skin is dried in this way is that the moisture content of keratinocyte layer is smaller than that of normal skin. In addition to this, even healthy skin may be in a harsh environment, such as wind, cold weather, sunshine, cleansing, shaving, etc., which causes water shortage, which can lead to poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer. To this end, cosmetics were added with ingredients similar to sebum, moisturizers such as NMF or polyol, and the like. For example, glycerin, solitol and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisture resistance, but they are very sticky and feel uncomfortable when used. Propylene glycol having two hydroxyl groups (OH groups) 3-butylene glycol, etc. may cause side effects of the skin. In addition, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic, which deteriorates emulsion stability of the cosmetic. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize.

The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

On the other hand, gold is a medicinal substance made from roots of scutellaria baicalensis, a perennial herbaceous plant belonging to the family Lamiaceae. It contains medicinal ingredients such as Baicalein, Baicalin, Wogonin, Wogonoside and is widely used for medicines. It is known that gold has antioxidant activity, stimulates bile secretion, inhibits gastric secretion and prevents arteriosclerosis, and has antibacterial and antifungal activity.

Leatiporus sulphureus var. Miniatus is a one-year-old brown-bellied mushroom that regenerates on the leaves, trees and stumps of conifers from spring to summer. Red duck mushrooms have long been reported to be effective in controlling function, promoting health, and preventing disease, but the exact pharmacological effect has not been well known. Red foxtail mushroom fruiting bodies include N-methylated tilamine derivatives, polysaccharides, lanosthenate triterpenoids, retinoic acid and various other mixtures. Recently, it has been reported that red mushroom has anticancer activity and insulin secretion control effect. Anticancer effect shows that extracellular polysaccharide of red mushroom suppresses cancer metastasis and enhances immunity. In addition, extracellular polysaccharide of red mushroom mycelium Are known to promote the production of skin cell growth factors and have wound healing and skin moisturizing effects. It is also known that red foxtail mushroom produces a large amount of functional enzymes including glucosidase.

On the other hand, there is little known about the efficacy of a golden red mushroom fermentation solution in which gold extracts, especially gold extracts, are fermented with mycelia of red mushroom. Accordingly, the inventors of the present invention have conducted an intensive study on the fermentation broth of golden red mushroom, and found that the skin moisturizing effect of the fermentation broth of golden red mushroom was confirmed as in Korean Patent No. 10-1375137 (registered on Apr. 31, 2014) The present invention has been completed on the basis of further studies on the efficacy of the fermented red mildew fermentation broth.

Korean Patent Laid-Open No. 10-2002-0061196 (published on July 24, 2002) discloses a cosmetic composition for inhibiting iNOS enzyme expression containing a golden extract. However, this does not disclose a cosmetic composition for preventing aging of skin, improving skin wrinkle, skin whitening, improving skin barrier function, improving skin inflammation, and improving atopic sensation, which contains a fermented product of golden red fungus as an active ingredient.

The present invention relates to a cosmetic composition for preventing skin aging, which is used as an effective ingredient for preventing skin aging, improving skin wrinkles, moisturizing skin, whitening skin, improving skin barrier function, improving skin inflammation and improving atopic appearance do.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a fermented extract of golden red mildew obtained by fermenting Scutellaria baicalensis extract with Laetiporus sulphureus mycelium CS0218 (KFCC 11494P) as an active ingredient to provide.

On the other hand, the gold extract can be produced using conventional solvents known in the art, that is, under the conditions of ordinary temperature and pressure, using conventional solvents. For example, extraction can be performed using one or more solvents selected from the group consisting of an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol.

Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the golden dry weight.

On the other hand, the fermented broccoli fermentation liquid of the present invention includes not only the extract obtained by the above extraction method, but also the extract obtained through a conventional purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The fraction is also interpreted to be included in the fermentation broth of golden red mushroom of the present invention.

In addition, the fermented product of the present invention can be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

According to a preferred embodiment of the present invention, the content of the fermentation broth of golden red mildew added to the cosmetic composition is 0.0001 to 100.0% by weight, preferably 0.001 to 90.0% by weight based on the total weight of the cosmetic composition, Is 0.1 to 85.0% by weight. When the content of the fermentation broth is less than 0.0001% by weight, the skin aging prevention effect, skin wrinkle improvement effect, skin moisturizing effect, skin whitening effect, skin barrier function improvement effect, skin inflammation improvement effect and atopic improvement effect are insignificant .

Meanwhile, the cosmetic composition of the present invention may contain, as an active ingredient, components commonly used in cosmetic compositions in addition to the fermented product of the above-described golden red mildew. For example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers may be included.

Meanwhile, the formulations of the cosmetic composition of the present invention are not limited to specific ones, and examples thereof include emulsions of lotion, gel, water-soluble liquid, cream, essence, oil-in-water type and w / A color cosmetic formulation consisting of an essential cosmetic formulation made of ointment, an underwater type and a water-in-oil type make-up base, a foundation, a skin cover, a lipstick, a lip gloss, a face powder, a two way cake, an eye shadow, a teak color and an eyebrow pencil One is good. It may also optionally be applied to the skin in aerosol form, in solid form, e.g. in the form of a stick, and as a skin care and / or makeup product.

On the other hand, the cosmetic composition may preferably be used for preventing skin aging.

In addition, the cosmetic composition may preferably be used for improving skin wrinkles.

In addition, the cosmetic composition may preferably be used for skin whitening.

In addition, the cosmetic composition may preferably be for improving skin inflammation.

In addition, the cosmetic composition may preferably be used for improving atopy.

The present invention relates to a method for improving the anti-aging effect of skin, improving skin wrinkles, skin moisturizing effect, skin whitening effect, skin barrier function improving effect, skin irritation improving effect and atopy improving effect, A cosmetic composition can be provided.

FIG. 1 is a graph showing free radical scavenging activity of a fermentation broth of a golden red foxtail mushroom.
FIG. 2 is a graph showing the anti-inflammatory effect of the fermentation broth of golden red mushroom.
FIG. 3 is a graph showing the effect of improving the skin wrinkles of the fermentation broth of golden red mushroom.
FIG. 4 is a graph showing skin whitening effect of a fermented product of a golden red foxtail mushroom.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[Preparation Example 1: Preparation of gold extract]

The gold was washed with purified water, dried and then pulverized, and a fine gold mill was prepared using a sieve of 100 mesh. 70% ethanol was added thereto so that the weight of the gold powder was 150 g / L, refluxed three times for 5 hours, and the mixture was cooled. The mixture was filtered through Whatman # 3 filter paper and concentrated under reduced pressure Dried to prepare a golden extract.

[Example 1: Preparation of fermentation broth of golden red mushroom]

To 5 g of the gold extract prepared in Preparation Example 1, 2% glucose was added as a carbon source, and then 0.3% peptone was further added to sterilize. Then, a culture medium of Laetiporus Sulphureus CS0218 (KFCC 11494P) was inoculated so as to have a concentration of 25 g / L. The cultures were incubated for 7 days at 37 ° C and pH 5 to 7 using a 5 L fermenter. After the culture, the culture broth was centrifuged to remove the culture medium first, and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture. Thereafter, ethanol was added to make a final 70% (v / v) ethanol aqueous solution, refluxed for 3 hours for 5 hours, cooled down, and filtered through a filter of whatman # 3. After filtration, the mixture was transferred to a concentration tank, concentrated under reduced pressure at 50 ° C or lower, and freeze-dried to prepare a fermented product of golden red mildew of the present invention.

[Comparative Examples 1 to 4: Preparation of Golden Extract Fermentation Liquid]

In this Example, the fermentation broth of gold extract was prepared by using the mycelium of mushroom described in Table 1 below. The preparation method was the same as in Example 1 except that the mycelial species of the mushroom was different.

mycelium Comparative Example 1 Matsutake Comparative Example 2 Mushroom Comparative Example 3 Situation mushroom Comparative Example 4 Gongji mushroom

[Experimental Example 1: Confirmation of antioxidative effect of fermented red mushroom fermentation broth]

In this experiment, the free radical scavenging activity was measured by the DPPH method in order to confirm the antioxidative effect of the fermented red mildew.

The DPPH assay measures absorbance at 540 nm to the extent that the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

Samples were prepared by adding the gold extract prepared in Preparation Example 1, the fermented product of the golden red mildew prepared in Example 1 and the fermented extract of the gold extract prepared in Comparative Examples 1 to 4 (0.001, 0.002, 0.005, 0.01% ). Amounts of the samples were as shown in Table 2, and 25 μM epigallocatechin gallate (EGCG) was used as a positive control. After the experiment, the free radical scavenging activity was calculated using the following equation (1).

Material Ctrol group Blank of C Exp. Group Blank of E A DPPH (MeOH) 180 μl (180) μl 180 μl (180) μl B Sample (solvent) (20) μl (20) μl 20 μl 20 μl

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

As a result of the experiment, the fermentation broth of the golden extract (Example 1 and Comparative Example 1 to 4) showed better free radical scavenging activity than the gold extract (Preparation Example 1). In particular, it was confirmed that the fermentation broth of golden red mushroom (Example 1) showed the highest free radical scavenging activity in the fermentation broth. In addition, the fermentation broth of the present invention showed a free radical scavenging activity equal to or higher than the free radical scavenging activity of the positive control group, epigallocatechin gallate (Fig. 1).

[Experimental Example 2: Evaluation of cell survival rate of fermented red mildew fermented broth]

In this experiment, cell viability was evaluated in order to examine the toxicity of the fermented red mushroom fermentation broth on cells.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) reagent.

Human fibroblast (HDF) was dispensed into a 96-well plate at a concentration of 1 × 10 ⁴ cells / well, and then cultured at 37 ° C. and 5% CO ₂ for 24 hours. After the culture, the medium was removed and the sample was cultured for 24 hours in a medium (0.001, 0.002, 0.005, 0.01%) treated with the concentration of the golden red mushroom fermentation broth prepared in Example 1, And washed twice with PBS (phosphate buffered saline). After that, MTT was dissolved in PBS at 5 mg / ml, and 50 L was added. Then, the cells were cultured at 37 ° C and 5% CO ₂ for 2 hours. After that, 100 L of DMSO was added per well, and the mixture was stirred for 10 minutes, and the absorbance was measured at 540 nm.

As a result, no cytotoxicity was observed at all concentrations of each sample. From this, it was confirmed that the fermented milk of the present invention is harmless to the human body and has excellent safety.

[Experimental Example 3: Confirmation of anti-inflammatory effect of fermented red mushroom]

In this experiment, 5-Lipoxygenase inhibitory activity test was carried out in order to confirm the anti-inflammatory effect of the fermentation broth of golden red mushroom.

Lipoxygenase produces a number of substances from arachidonic acid that act as various chemical mediators involved in inflammatory and allergic reactions in vivo. The inhibitory activity of lipoxygenase is an experiment in which the degree of peroxide formation is measured using a substrate and an enzyme.

Samples were prepared by adding the gold extract prepared in Preparation Example 1, the fermented product of the golden red mildew prepared in Example 1 and the fermented extract of the gold extract prepared in Comparative Examples 1 to 4 (0.001, 0.002, 0.005, 0.01% ), And the amounts of the samples were as shown in Table 3 below. Also, 250 nM NDGA (Nordihydroguaiaretic acid) was used as a positive control.

Material Ctrol group Blank of C Exp. Group Blank of E A Buffer 2 ml 2 ml 2 ml 2 ml B Sample (solvent) (20 [mu] L) (20 [mu] L) 20 μl 20 μl C Enzyme 40 μl - 40 μl - D Substrate 70 μl 70 μl 70 μl 70 μl

As a result, the gold extract fermentation broth (Example 1 and Comparative Examples 1 to 4) showed excellent 5-lipoxygenase inhibitory activity as compared to the gold extract (Preparation Example 1). In particular, it was confirmed that the fermentation broth of golden red mushroom (Example 1) exhibited the highest 5-lipoxygenase inhibitory activity in the fermentation liquid of gold extract. In addition, the fermentation broth of the present invention showed 5-lipoxygenase inhibitory activity equivalent to or higher than the 5-lipoxygenase inhibitory activity of the positive control nordihydroguaiaretic acid (Fig. 2).

[Experimental Example 4: Confirmation of wrinkle-improving effect of fermented red mildew fermented broth]

In order to confirm the wrinkle-reducing effect of the fermentation broth of golden red mushroom, the experiment was carried out on the substrate metalloproteinase (MMP-1) inhibitory activity after irradiation with ultraviolet light.

Samples were prepared by adding the gold extract prepared in Preparation Example 1, the fermented product of the golden red mildew prepared in Example 1 and the fermented extract of the gold extract prepared in Comparative Examples 1 to 4 (0.001, 0.002, 0.005, 0.01% ).

Human fibroblast (HDF) was irradiated with UVA at an energy of 5 J / cm < ³ > using a UV chamber. Ultraviolet irradiation dose and incubation time were established by preliminary experiment to maximize MMP expression level in fibroblasts. Negative controls were wrapped in silver foil and kept in the UVA environment for the same amount of time. UVA emission was measured using a UV radiometer. The UVA-irradiated cells were irradiated with UVA, exchanged with a medium containing 500 μl of the sample, and cultured for 24 hours. Then, the medium was recovered and coated on a 96-well plate. Subsequently, 100 μl of the primary antibody (MMP-1 (Ab-5) monoclonal antibody and 100 μl of MMP-2 (Ab-3) monoclonal antibody) was treated and reacted at 37 ° C for 60 minutes. Then, 100 μl of alkaline phosphatase substrate solution (1 mg / ml-nitrophenyl phosphate in diethanolamine buffer solution) was reacted at 30 ° C for 30 minutes at room temperature, followed by incubation with a secondary antibody, anti mouse IgG And the absorbance at 405 nm was measured with a microplate reader. As a control group, no sample was used, and 1 mg / ml retinol was used as a positive control.

As a result, the golden extract fermentation broth (Example 1 and Comparative Examples 1 to 4) showed excellent MMP-1 expression inhibition ability compared to the gold extract (Preparation Example 1). In particular, it was confirmed that the fermentation broth of golden red mushroom (Example 1) showed the highest MMP-1 expression inhibiting ability in the fermentation liquid of the golden extract. In addition, the ferroalloy extract of the present invention showed an inhibitory effect on MMP-1 expression equal to or higher than the inhibitory effect of retinol on MMP-1 expression (FIG. 3).

[Experimental Example 5: Confirmation of skin whitening effect of fermented liquid of golden red mushroom]

In this experiment, a tyrosinase inhibitory activity test was carried out in order to confirm the skin whitening effect of a fermented red mildew fermentation broth.

Samples were prepared by adding the gold extract prepared in Preparation Example 1, the fermented product of the golden red mildew prepared in Example 1 and the fermented extract of the gold extract prepared in Comparative Examples 1 to 4 (0.001, 0.002, 0.005, 0.01% ).

Tyrosinase (EC 1.14.1.1) was dissolved in 0.05 M phosphate buffer (pH 6.5) to a final volume of 1,100 unit / ml, and the substrate L- Tyrosine (C ₉ H ₁₁ NO ₃ , 181.19) was dissolved in 0.1 M phosphate buffer (pH 6.5) to a final concentration of 225 μM, and the cells were washed with 0.1 M sodium phosphate buffer , pH 6.5), reacted at 37 ° C for 20 minutes, and then absorbance was measured using an ELISA reader at 492 nm. Then, tyrosinase inhibitory activity was calculated using the following formula (2). On the other hand, 50 μM kojic acid was used as a positive control.

Material Ctrol group Blank of C Exp. Group Blank of E A Buffer 140 μl 150 μl 140 μl 150 μl B Sample (solvent) 20 < RTI ID = 20 < RTI ID = 20 μl 20 μl C Enzyme 10 μl - 10 μl - D Substrate 30 μl 30 μl 30 μl 30 μl

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

As a result of the experiment, the golden extract fermentation broth (Example 1 and Comparative Examples 1 to 4) showed excellent tyrosinase inhibitory activity as compared to the gold extract (Preparation Example 1). In particular, it was confirmed that the fermentation broth of golden red mushroom (Example 1) showed the highest tyrosinase inhibitory activity in the fermentation liquid of gold extract. In addition, the golden ferret extract of the present invention showed tyrosinase inhibitory activity equal to or higher than that of tyrosinase inhibitory activity of the positive control kojic acid (FIG. 4).

[Preparation Example 2: Preparation of a cosmetic composition containing a fermented liquid of golden red foxtail mushroom]

In this Example, a cosmetic composition containing a fermented liquid of golden red mushroom was prepared.

The composition of the cosmetic composition containing the fermented product of the golden red mildew produced in Example 1 was referred to as Prescription Example 1 and the cosmetic composition containing no fermented product of golden red mushroom was referred to as Comparative Prescription Example 1. The composition of the cosmetic composition was as shown in Table 5 below.

ingredient Prescription Example 1
Content (% by weight) Comparative Prescription Example 1
Content (% by weight) Example 1 30.0 - 1,3-BG 10.0 10.0 glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia nut oil 2.5 2.5 Polyahyuvait 60 1.6 1.6 Azithromycin 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl vinyl polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

[Experimental Example 6: Confirmation of Skin Moisturizing Effect of Cosmetic Composition Containing Fermentation Liquid of Golden Red Delicious Fungus]

In this Experimental Example, the skin moisturizing effect of the cosmetic composition containing the fermentation liquid of golden red mushroom was examined.

Twenty-five to twenty-four to twenty patients without skin disease were divided into two groups of 20 persons per one group, and the cosmetic composition of Prescription Example 1 and Comparative Prescription Example 1 prepared in Preparation Example 2 was administered twice daily for one month And spread on the forehead. The skin conductivity was measured by a corneometer (CM820 courage Khazaka electronic GmbH, Germany) in a constant temperature and humidity condition (24 ° C, 40% humidity) The rate of increase (%) of the skin conductivity after the main course was measured and the rate of increase was evaluated. The results are shown in Table 6 below.

sample After 1 week after 2 weeks After 4 weeks Prescription Example 1 55% 59% 64% Comparative Prescription Example 1 26% 30% 36%

As a result of the experiment, it was confirmed that in the case of Formulation Example 1, which is a cosmetic composition containing the fermented product of golden red foxtail of the present invention, the rate of skin conductivity increase is much superior to Comparative Prescription Example 1. That is, it was confirmed that the skin moisturizing effect of the cosmetic composition containing the fermented red mildew of the present invention was excellent.

[Experimental Example 9: Confirmation of atopy improvement effect of a cosmetic composition containing a fermented liquid of golden red mushroom]

In this Experimental Example, the effect of improving the atopy of the cosmetic composition containing the fermentation broth of golden red mildew produced in Preparation Example 2 was examined.

Experiments with Atopy 20 persons aged from 3 to 40 years old were administered the cosmetic composition of Prescription Example 1 prepared in Preparation Example 2 and the cosmetic composition of Comparative Prescription Example 1 to 10 patients, Twice a day for 4 consecutive weeks. Atopic dermatitis severity, IgE change, coral leucocyte (Eosinophil) change, moisture content and skin acidity were measured before and after the application of the cosmetic composition (0 week) and after 4 weeks of application of the cosmetic composition.

Atopic dermatitis severity was assessed by visual observation for 4 weeks, then 0 points: no symptoms (none)

10 points: weak 20 points: mild symptoms (mild) 30 points: severe symptoms (severe) 40 points: very severe symptoms (severe, extremely severe) gave the score .

In addition, the amount of total IgE was measured using an ELISA kit (Yamasa, Tokyo, Japan) for immunoglobulin (IgE) change.

In addition, coral leukocyte changes were measured by counting using a hemocyte automatic meter.

In addition, the water content was measured using a moisture meter (Corneometer 825).

Skin acidity was also measured using a skin pH meter.

On the other hand, no adverse events were observed in all subjects participating in the 4-week test, and the results were as shown in Table 7 (n = 20, p < 0.05).

division Atopic dermatitis severity (index) IgE change
(mg / ml) Coral leukocyte change (ea) Moisture content
(g / cm < ² > h) Skin acidity
(pH) I'm after I'm after I'm after I'm after I'm after Prescription Example 1 37.41 28.12 435.9 321.4 317 249 25.84 26.57 5.719 6.071 Comparative Prescription Example 1 37.68 31.53 443.7 363.8 321 275 26.31 25.11 5.717 5.833

As a result of the test, the cosmetic composition (Prescription Example 1) containing the golden red cruciferous fermentation broth of the present invention such as atopic dermatitis severity, atopic disease dermatitis severity, blood IgE and coral leukocyte change, water content, skin acidity, It was confirmed that the cosmetic composition containing the fermentation broth of golden red mildew of the present invention had an excellent atopy improving effect.

Claims (1)

A cosmetic composition for improving skin inflammation and atopic eczema, which contains, as an active ingredient, a fermented extract of golden red mildew obtained by fermenting a golden extract ( Scutellaria baicalensis ) with Laetiporus sulphureus mycelium CS0218 (KFCC 11494P).

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