KR101997231B1 - Cosmetic composition containing enzyme-treated honey extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an enzyme-treated honey extract as an active ingredient. Provided according to the present invention is a cosmetic composition containing, based on the total weight of the cosmetic composition, 0.01 to 15 wt% of a honey extract prepared by treating a mixed enzyme of naringinase and pectinase. The enzyme-treated honey extract of the present invention not only has excellent skin exfoliation effect, skin moisturizing effect, whitening effect, wrinkle reduction and skin tone improvement effect, but also has an excellent effect of enhancing the activity of useful ingredients in skin used together in the formulation.

Description

[0001] The present invention relates to a cosmetic composition containing an extract of a honey produced by an enzyme reaction,

The present invention relates to a process for preparing a honey extract and using it as a cosmetic, and more particularly, to a process for producing an extract of honey by an enzyme reaction and a method for producing the honey extract, An improvement effect and an effect of raising the activity of a skin-useful component.

Aging is a process in which the structure and function of the body gradually deteriorate as the years pass, and the sensitivity to diseases and death increases rapidly, resulting in physiological changes in the body resulting in remarkable degradation throughout the body . Among them, it is recognized that skin aging is the most important thing that we can easily feel as it is as important as diabetes, heart disease, arteriosclerosis, dementia and cancer. There are individual differences but skin aging usually starts around the early 20s Of the total population.

In order to delay such aging process, skin-improving substances showing various functions have been developed and used. However, in the case of cosmetics, the physiologically active substance contained in cosmetics is only required to pass through the stratum corneum which is the outermost layer of the skin. However, the skin improving substance, which is a useful ingredient of skin contained in cosmetics due to the skin barrier function, And therefore, it has a problem that it can not exhibit its activity. In order for such a skin improving substance to exhibit its function, it should be contained at a high concentration. In this case, the price of the product is increased, and the possibility of inducing skin irritation by high concentration is increased. Therefore, in order to overcome such a problem, it is necessary to develop a cosmetic for accelerating skin absorption, which can stably add a small amount of skin improving substance to the cosmetic and effectively exhibit its function.

Skin permeation accelerators have been reported to promote skin penetration of active substances through various studies. Their permeation enhancement is largely attributed to i) intercellular lipid bilayer, ii) desmosome involved in intercellular binding, desmosomes and related proteins, and iii) mechanisms that act directly on the keratinocyte internal structure. These alter the structure of the horny layer, which is the outermost layer of the skin, to promote the skin permeation of the physiologically active substance. As a result, most of the skin penetration enhancers have high cytotoxicity to skin cells, and are highly likely to induce skin irritation such as skin burning and erythema upon application.

Under these circumstances, the inventors of the present invention have studied a method for increasing the activity of skin oil-soluble components showing skin-improving effects. As a result, when using a honey extract prepared by enzyme treatment with a specific method, The present invention has been accomplished on the basis of the fact that it is excellent in moisturizing effect, whitening effect, wrinkle improving effect and skin tone improving effect, and also has an effect of raising the activity of a skin benefit component in a cosmetic composition without skin irritation.

(001) Korean Patent Publication No. 10-2016-0049812 (May 10, 2016). (002) Korean Patent Publication No. 10-2018-0063394 (Jun.

The present invention relates to a cosmetic composition containing honey extract prepared using an enzyme reaction and having an effect of enhancing skin exfoliating effect, skin moisturizing effect, whitening effect, wrinkle improving effect, skin tone improving effect, The purpose is to provide.

It is another object of the present invention to provide a method for producing an extract of honey from which the honey is treated with a specific enzyme to produce an extract.

To achieve the above object, according to the present invention, there is provided a cosmetic composition comprising 0.01 to 15 wt% of a honey extract prepared by treating a mixed enzyme of naringinase and pectinase, based on the total weight of the cosmetic composition / RTI >

The mixed enzyme is composed of naringinase and pectinase in a weight ratio of 1: 3: 1 to 3: 1.

More preferably, the enzyme treatment is carried out under the conditions of a reaction pH of 4.5, a reaction temperature of 48 ° C and a reaction time of 48 hours.

The honey extract is characterized by exhibiting an effect of enhancing the activity of a useful component of the skin contained in the cosmetic composition.

The skin benefit component is at least one selected from the group consisting of adenosine, arbutin and hyaluronic acid.

The cosmetic composition is characterized in that it is used for skin exfoliation, skin moisturizing, whitening, skin wrinkle improvement or skin tone improvement.

According to the present invention, there is provided a process for producing honey, comprising the steps of (A) mixing honey with at least one solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol ; (B) filtration, followed by concentration under reduced pressure and drying to prepare a honey extract powder; (C) adding purified water to the honey solvent extract powder, adding a mixed enzyme comprising naringinase and pectinase in a weight ratio of 1 to 3: 1 to 3, respectively; (D) stirring the enzyme reaction at pH 4.5 and 48 ° C for 48 hours with stirring; And (E) sterilization, followed by concentration under reduced pressure and lyophilization.

The honey extract of the present invention produced using an enzyme reaction has excellent skin exfoliation effect, skin moisturizing effect, whitening effect, wrinkle improvement and skin tone improvement effect, So that it is possible to expect an excellent skin improving effect even if the content of the useful ingredient of the skin is reduced. Therefore, the honey extract prepared using the enzyme reaction of the present invention can be usefully used as an anti-aging cosmetic.

1 is a graph showing hyaluronic acid production effect of a honey extract according to an embodiment of the present invention.
2 is a graph showing the effect of promoting HAS mRNA expression of a honey extract according to an embodiment of the present invention.
FIG. 3 is a photograph showing the effect of extracting honey on the skin of the present invention. FIG.

Hereinafter, the present invention will be described in more detail.

"Honey" refers to the storage and conversion of honey and pollen collected from flowers of various plants into their foodstuffs. It contains glucose, fructose, carbohydrates, minerals, proteins, peptides, Enzyme and yeast. Honey has long been used for nourishing gums, soothing, detoxifying, restoring fatigue, gastrointestinal disorders, coughing, dry skin, eczema, constipation, nervous breakdown, reducing blood sugar and maintaining metabolic balance. It has a beneficial effect on beauty and health and is known to be rich in vitamin B group, especially B6.

The present invention relates to a technique for preparing an extract of honey using an enzyme reaction and using it as a cosmetic.

According to the present invention, there is provided a cosmetic composition comprising a honey extract prepared by treating a mixed enzyme of naringinase and pectinase in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition. The mixed enzyme is a mixture of naringinase and pectinase at a weight ratio of 1 to 3: 1 to 3, more preferably 1: 2 to 3, And most preferably in a weight ratio of 1: 2.

More preferably, the enzyme treatment is carried out under the conditions of a reaction pH of 4.5, a reaction temperature of 48 ° C and a reaction time of 48 hours.

According to one embodiment of the present invention, the enzyme-treated honey extract is prepared as follows. First, honey is extracted with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol. The honey solvent extract is treated with a mixed enzyme of Naringinase and Pectinase and reacted to prepare a honey extract. At this time, the mixed enzyme is prepared by mixing Naringinase and Pectinase at a weight ratio of 1: 3: 1 to 3, respectively. More preferably, the enzymatic reaction is carried out by adding a mixed enzyme of naringinase and pectinase to the honey extract of the present invention and stirring the reaction mixture at a reaction pH of 4.5, a reaction temperature of 48 ° C, and a reaction time of 48 hours Honey extract is prepared.

According to another aspect of the present invention, there is provided a honeycomb structure comprising (A) at least one honeycomb selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol Of a solvent; (B) filtration, followed by concentration under reduced pressure and drying to prepare a honey extract powder; (C) adding purified water to the honey solvent extract powder, adding a mixed enzyme comprising naringinase and pectinase in a weight ratio of 1 to 3: 1 to 3, respectively; (D) stirring the enzyme reaction at pH 4.5 and 48 ° C for 48 hours with stirring; And (E) sterilization, followed by concentration under reduced pressure and lyophilization.

It was confirmed that the honey extract prepared by the above-described method exhibits superior moisturizing effect, wrinkle improvement, skin tone improvement effect and exfoliating effect in comparison with simple honey extract, lactic acid bacteria fermented honey extract and yeast fermented honey extract.

Moreover, the honey extract prepared by the above-described method showed an effect of enhancing the activity of other useful ingredients of the skin contained in the formulation.

When the effectiveness of the skin-improving active ingredient used in cosmetics is inferior in efficiency, it is inevitable to use a large amount of the skin-improving agent in order to obtain a desired skin improving effect, which may cause irritation of the skin along with an increase in cost. It has been found that the honey extract of the present invention produced using the enzyme reaction has an effect of promoting skin absorption and enhancing the activity of useful ingredients of skin such as adenosine, arbutin and hyaluronic acid used together. This means that even if a small amount of active ingredient is used, desired skin improving effect can be obtained.

The honey extract prepared by the enzyme reaction is contained as an active ingredient in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention can be produced in the form of a general emulsified formulation and a solubilized formulation. Cosmetics of emulsified form include nutrition lotion, cream, essence, etc., and cosmetics of solubilized form have softening longevity. In addition to cosmetics, it can be prepared in the form of adjuvants which can be applied locally and systemically, which are conventionally used in the field of dermatology, by containing dermatologically acceptable mediums and bases.

The formulation of the cosmetic composition according to the present invention is not particularly limited, but may be applied to various cosmetic compositions such as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, And may be applied to any one form selected from the group consisting of a pack, a soap, a shampoo, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a latex, a press powder, a loose powder and an eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Production Example 1: Preparation of Solvent Extract of Honey

The stock solution of acacia honey was put into purified water of 10 times by weight, extracted at 90 DEG C for 2 hours with stirring, cooled and filtered. The concentrate was concentrated at 50 ~ 60 ℃ using a vacuum concentrator and then lyophilized with a freeze dryer to prepare an extract powder.

Production Example 2: Preparation of lactic acid bacteria fermented honey extract

8 times the weight of purified water was added to the honey extract prepared in Preparation Example 1, and the culture solution of Lactobacillus sakei was inoculated at 2% (v / v), followed by reaction at 27 ° C for 48 hours. Then, it was sterilized at 90 DEG C for 5 minutes, cooled and filtered, and then concentrated at a temperature of 50 to 60 DEG C using a vacuum concentrator. And lyophilized with a freeze dryer to prepare lactic acid fermented honey extract.

Production Example 3: Preparation of yeast fermented honey extract

Eight times as much water as purified water was added to the honey extract prepared in Preparation Example 1 to inoculate 2% (v / v) of yeast Saccharomyces cerevisiae, followed by reaction at 35 ° C for 48 hours . The reaction product was sterilized at 90 DEG C for 5 minutes, cooled and filtered, and then concentrated at a temperature of 50 to 60 DEG C using a vacuum condenser. And then lyophilized with a freeze dryer to prepare yeast fermented honey extract.

Examples 1 to 7: Preparation of enzyme reaction honey extract

Eight times as much water as purified water was added to the honey extract prepared in Preparation Example 1, and the enzyme reaction was carried out with stirring at pH 4.5 and 48 ° C for 48 hours. Thereafter, the reaction product was sterilized at 90 캜 for 5 minutes, concentrated under reduced pressure and lyophilized to prepare a honey extract.

Classification (weight ratio) Enzyme Naringinase Pectinase Example 1 One - Example 2 - One Example 3 One One Example 4 One 2 Example 5 One 3 Example 6 2 One Example 7 3 One

Test Example 1: Test for cytotoxicity

The cytotoxicity of skin cells by the samples of honey extract prepared in the above Preparation Examples and Examples was confirmed. Human-derived fibroblasts were cultured in 96-well plates at a concentration of 1 × 10 ⁶ cells / well, and 0.2 ml of IMDM medium containing 10% FBS was added thereto, followed by culture in a CO ₂ incubator for 24 hours. The sample solution dissolved in DMSO was treated and cultured for 24 hours. After that, MTT assay was performed to examine cell viability. For MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. Then, the medium was removed, and 0.5 ml of DMSO was added to the formed formazan, and the absorbance was measured at 570 nm using ELISA. Cell viability (%) was measured by the following equation, and the results are shown in Table 2 below.

Cell survival rate (%) = [(St-Bo) / (Bt-Bo)] 100

Bo: absorbance at 570 nm of wells that had undergone chromogenic reaction in cell culture medium

Bt: Absorbance at 570 nm of the well that underwent chromogenic reaction without treatment of the sample

St: absorbance at 570 nm of the well treated and chromogenic

division Cell survival rate (%) 0.1% 0.5% 1.0% Production Example 1 100 95 88 Production Example 2 100 97 94 Production Example 3 100 98 95 Example 1 100 95 91 Example 2 100 99 96 Example 3 100 97 92 Example 4 100 100 99 Example 5 100 100 97 Example 6 100 100 93 Example 7 100 100 91

As can be seen from the above Table 2, high cell viability was confirmed in all of the samples to which the extracts of Preparation Example and Example were applied, and it was confirmed that the possibility of causing skin irritation was low and safe because of low influence on cytotoxicity.

Test Example 2: Measurement of hyaluronic acid production

The amounts of hyaluronic acid produced by the samples of the honey extracts prepared in the above Preparation Examples and Examples were Respectively. HaCaT cells were cultured in a 96-well plate at a concentration of 1 × 10 ⁶ cells / well, starved for 18 hours, and cultured for 24 hours. HaCaT cells were treated with 1.0% of each sample, cultured for 24 hours, and the amount of hyaluronic acid (HA) produced was measured using an ELISA kit. The results are shown in Fig.

As can be seen from FIG. 1, the honey extract of the present invention, which was obtained by treating a mixed enzyme of naringinase and pectinase, showed a much higher amount of HA than that of the general solvent extract. In comparison with the lactic acid bacteria and yeast fermented extract And showed excellent effects. Among them, the sample of Example 4 in which Naringinase and Pectinase were mixed at a ratio of 1: 2 and subjected to an enzymatic reaction showed the best hyaluronic acid producing effect.

Test Example 3: Increased expression of HAS mRNA

The amounts of HAS mRNA expressed by the honey extract samples prepared in the above Preparation Examples and Examples were Respectively. HaCaT cells were cultured in a 96-well plate at a concentration of 1 × 10 ⁶ cells / well, starved for 18 hours, and cultured for 24 hours. HaCaT cells were treated with 1.0% of each sample and cultured for 24 hours. Then, hyaluronic acid synthase (HAS) mRNA expression was measured by RT-PCR. The HAS-3 expression increase rate was calculated by the following equation, and the results are shown in FIG.

(%) = (Expression amount of sample HAS-3 / expression amount of control HAS-3) × 100

As shown in FIG. 2, the honey extract of the Example of the present invention, which was obtained by treating a mixed enzyme of Naringinase and Pectinase, showed a much higher increase in the expression of HAS-3 than that of a normal solvent extract, The samples of Examples 4 and 5, in which the enzyme reaction was carried out by mixing pectinase 1: 2 to 3, showed excellent effects as compared with those of lactic acid bacteria and yeast fermentation extracts.

Test Example 4: Test for inhibiting MMP-1 production

Human normal fibroblasts (CCD-986sk cells) were inoculated on a 96-well plate at 1 × 10 ⁶ cells / well and cultured for 24 hours. The samples of the above Preparation Examples and Examples were added to serum-free DMEM medium at different concentrations, and the cells were further cultured for 24 hours to obtain a cell culture medium. The amount of MMP-1 in the cell culture medium was measured using a MMP-1 assay kit (Amersham, USA) by taking the culture medium, and the inhibition rate of MMP-1 production was calculated according to the following formula. At this time, adenosine was used as a positive control for the inhibition rate of MMP-1 production.

sample
density
(%) Inhibition rate of MMP-1 production (%) Manufacturing example Example Adenosine One 2 3 One 2 3 4 5 6 7 0.1 12.1 18.9 19.4 26.5 45.7 40.7 47.7 46.8 44.1 32.3 46.8 0.5 23.8 35.7 40.4 44.1 61.8 60.0 68.9 64.5 58.6 44.4 64.2 1.0 35.9 44.0 53.8 60.6 80.2 78.9 85.7 83.5 80.8 67.8 83.3

As shown in Table 3 above, the samples of the examples treated with the two enzymes of naringinase and pectinase exhibited excellent overall MMP-1 expression inhibitory effect.

Test Example 5: Test for promoting collagen biosynthesis

Human normal fibroblasts (CCD-986sk cells) were inoculated on a 96-well plate at 1 × 10 ⁶ cells / well and cultured for 24 hours. The extracts of the above Preparation Examples and Examples were added to serum-free DMEM medium at different concentrations, and the cells were further cultured for 24 hours to obtain a cell culture medium. The amount of collagen in the cell culture was quantitated using procollagen type I C-peptide (PIP) EIA kit (Takara Bio, Japan). The amount of collagen measured was corrected to the total amount of protein determined by the Lowry assay. The results are shown in Table 4 below. As a control group, adenosine was used.

sample
density
(%) procollagen expression rate (%) Manufacturing example Example Adenosine One 2 3 One 2 3 4 5 6 7 0 2.6 2.6 2.6 2.6 2.6 2.6 2.6 2.6 2.6 2.6 2.6 0.1 8.7 9.9 11.3 18.4 22.9 13.8 24.7 23.1 22.3 20.8 27.4 0.5 18.4 17.7 25.2 33.1 38.0 28.4 42.9 41.0 36.7 31.5 43.9 1.0 28.8 34.9 48.2 47.8 50.4 44.7 58.3 54.8 48.4 41.0 59.7

As shown in Table 4, the samples of the examples in which the enzymes of two kinds of naringinase and pectinase were mixed, showed excellent collagen production promoting effect overall. Among them, naringinase and pectinase enzyme Were mixed at a ratio of 1: 2, the sample of Example 4 exhibited the best promoting effect of collagen production.

Test Example 6: Skin cell regeneration effect

A wound healing assay was performed to confirm the skin regeneration effect of the honey extracts prepared in the above Preparation Examples and Examples. The keratinocyte (HACAT) was adjusted to 1 × 10 ⁶ cells / well using DMEM supplemented with 10% FBS, and then inoculated in a 6-well plate and cultured for 18 hours. The cells grown in a 6-well plate were scratched to allow a portion of the cells to fall off the bottom of the plate, and the samples of each of the examples and the preparations were treated with 0.5 (w / v%) for 48 hours . The degree of cell regeneration was confirmed, and the cell regeneration rate was calculated by the following equation.

Cell regeneration rate (%) = (sample cell regeneration / control cell regeneration) x 100

The calculated cell regeneration rates are shown in Table 5 below. Dexamethasone was used as a control.

division Treatment concentration (w / v%)  Cell Regeneration (%) Production Example 1 1.0 57.9 Production Example 2 1.0 60.6 Production Example 3 1.0 66.3 Example 1 1.0 69.7 Example 2 1.0 79.9 Example 3 1.0 71.4 Example 4 1.0 82.5 Example 5 1.0 80.1 Example 6 1.0 79.5 Example 7 1.0 77.8 Dexamethason 1.0 83.2

As shown in Table 5, the honey extract of the example of the present invention, which was obtained by treating the mixed enzyme of naringinase and pectinase, exhibited remarkably superior cell regeneration effect than the general solvent extract, and the lactic acid bacteria, yeast fermented extract Which is superior to the conventional method.

Test Example 7: Test for inhibiting tyrosinase activity

The tyrosinase inhibitory activity of the honey extract prepared in the above Preparation Examples and Examples was evaluated by Respectively. B16F10 cells were treated with α-melanocyte stimulating hormone (α-MSH) and the samples of the above-mentioned preparation examples and the examples and cultured for 72 hours. After culturing, the cells were washed with phosphate buffered saline (pH 7.4), centrifuged to obtain a cell suspension, 100 μL of 10 mM phosphate buffer (pH 6.8) containing 1% (w / v) triton X-100 was added and shaking After the cells and solution were transferred to an eppendorf tube, the supernatant was used for tyrosinase activity measurement. The supernatant obtained after the sample treatment was dispensed in a 96-well plate and 40 μL of 10 mM L-DOPA dissolved in 0.1 M sodium phosphate buffer (pH 7.2) was added and incubated at 37 ° C. for 30 minutes. The absorbance of DOPA chrome produced by tyrosinase was measured at 475 nm. The inhibitory activity of each extract against the inhibitory activity of tyrosinase of the control (con) treated with Ascorbic acid, which is a positive control group, was confirmed and the results are shown in Table 6.

sample
density
(%) Inhibition ratio (%) Manufacturing example Example Ascorbic acid One 2 3 One 2 3 4 5 6 7 0.1 13.7 19.9 18.4 28.4 32.9 21.3 35.2 33.2 23.9 20.1 48.7 0.5 18.8 27.7 24.5 36.1 48.0 33.8 53.4 50.0 43.6 31.5 59.4 1.0 32.8 32.9 40.4 56.3 58.2 44.4 61.8 59.7 49.9 47.0 70.1

As can be seen in Table 6, the honey extract of the present invention, which was obtained by treating a mixed enzyme of naringinase and pectinase, reacted to exhibit excellent tyrosinase inhibitory activity as compared with general solvent extract, lactic acid bacterium, and yeast fermented extract .

Test Example 8: Test for inhibiting melanin formation

The amounts of melanin produced by the samples of the honey extracts prepared in the above Preparation Examples and Examples were measured. Melanoma cell B16F10 was treated with ascorbic acid, a positive control, to determine the amount of melanin production. Melanoma cell B16F10 was inoculated on a 96-well plate at a concentration of 1.0 × 10 ⁶ cells / well and treated for 3 days. After 3 days, the medium of each well was removed, washed with PBS, 1 mL of 1 N NaOH was added to each well, and the mixture was stirred to dissolve the melanin component. The absorbance was measured at 400 nm using a microplate reader to measure the content of dissolved melanin. The results are shown in Table 7.

sample
density
(%) Melanin production (%) Manufacturing example Example Ascorbic acid One 2 3 One 2 3 4 5 6 7 0.1 99.6 96.2 94.4 94.3 90.3 92.3 88.3 90.0 93.9 92.0 88.7 0.5 91.3 89.9 89.7 83.1 79.4 88.5 73.6 78.8 85.2 84.7 74.1 1.0 79.8 74.4 76.5 68.7 62.1 73.9 59.7 63.4 70.6 69.4 60.3

As shown in Table 7, the honey extract of the present invention showed excellent whitening activity as compared with general solvent extract, lactic acid bacteria and yeast fermented extract, and showed the most excellent effect in Example 4.

Example 8: Preparation of a cream containing an enzyme-converted honey extract

The cream containing the extract of Example 4 was prepared according to a conventional method with the composition and content shown in Table 8 below.

ingredient Content (% by weight) Control Example 1 Example 8 Honey extract (Example 4) - 2.0 Pro-type glycerin monostearate 2.0 2.0 Stearyl alcohol 2.2 2.2 Stearic acid 1.5 1.5 Wax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan stearate 0.6 0.6 Purified vegetable oil 1.0 1.0 Squalane 3.0 3.0 Mineral oil 5.0 5.0 Trioctanoin 5.0 5.0 Dimethicone 1.0 1.0 Sodium magnesium silicate 0.1 0.1 glycerin 5.0 5.0 Betaine 3.0 3.0 Triethanolamine 1.0 1.0 Sodium hyaruronate 4.0 4.0 Preservative, fragrance, pigment a very small amount a very small amount Purified water Balance Balance Sum 100 100

Test Example 9: Confirmation of skin horny improving effect

In order to confirm the effect of improving the skin's exfoliation, each of 10 persons was applied for 4 weeks twice a day (morning and evening) using the formulations of Example 8 and Control 1. After 4 weeks, the degree of improvement of the keratin was evaluated by image analysis.

Before the start of the experiment, the horny part of the facial part to be measured was measured, and after applying for 4 weeks (twice / day), the horny state of the skin was measured by the horny light and darkness analysis through image analysis. The measurement results of the skin horny condition by image analysis are shown in Fig.

As shown in FIG. 3, the cream of Example 8 containing the enzyme-converted honey extract of the present invention exhibited excellent exfoliation improving effect.

Test Example 10: Confirmation of skin moisturizing effect

Skin moisturization was evaluated by measuring the skin conductivity using a skin moisture meter (Corneometer, Courage + Khazaka, GmbH, Germany) as a clinical test for skin moisturization. The skin moisture was measured by subjecting the cream of Example 8 and the cream of Comparative Example 1 to half of the subject's face in half under constant temperature and humidity conditions maintained at a room temperature of 20 to 25 캜 and a relative humidity of 40 to 55% , The water content of the control sample was measured before and two weeks after the application of the control sample twice / once a day. The results are shown in Table 9 below.

division  The initial (D0) average 2 weeks (D14) Average Moisture Increase (%) Control Example 1 32 36 13 Example 8 33 44 33

As can be seen in Table 9, high moisturizing effect was shown in Example 8 containing the enzyme-converted honey extract of the present invention.

Test Example 11: Confirmation of the effect of improving skin wrinkles

In order to confirm the effect of improving the wrinkles of the skin, the formulations of Example 8 and Comparative Example 1 were applied to the face portion for 2 weeks (morning and evening) for 4 weeks for each 10 persons. After 4 weeks, the improvement of wrinkles was evaluated by image analysis of wrinkles.

The evaluation of the wrinkles by the image analysis was performed by taking replica under the eyes before the start of the experiment and collecting the replica immediately after the end of the experiment from the same area under the eyes and by analyzing the image by two dimensional analysis of the wrinkles, Were measured. The results of the measurement of the wrinkle density by image analysis are shown in Table 10 below as a reduction rate relative to the wrinkle density before use.

division Wrinkle density reduction rate (%) Control Example 1 4 Example 8 21

As shown in Table 10, the wrinkle-improving effect was shown in Example 8 containing the enzyme-converted honey extract of the present invention.

Test Example 12: Confirmation of skin tone improvement effect

In order to confirm the effect of improving the skin tone, the formulations of Example 8 and Control Example 1 were applied to each of 10 persons for 4 weeks twice a day (morning and evening) on the face. Before the start of the experiment, the skin tone to be measured was measured, and after applying for 4 weeks (twice / day), the tone improvement degree of the skin was measured using a colorimeter. L *, a *, and b * color systems are used to represent color, and L * value (brightness) is used as an index in this experiment. L * values are from black (L * = 0) to white (L * = 100), and ΔL * after 4 weeks is calculated. The results are summarized in Table 11 below.

division DELTA L * Control Example 1 0.43 Example 8 7.97

As shown in Table 11, the skin tone improvement effect was high in Example 8 containing the enzyme-converted honey extract of the present invention.

Examples 9 to 11: Production of a cream containing an enzyme-converted honey extract and a skin-active ingredient

The extract of Example 4 and the cream containing the functional ingredient, which is a useful ingredient for the skin, were prepared according to the conventional methods with the composition and contents shown in Table 12 below.

ingredient Content (% by weight) Comparative Example Example One 2 3 9 10 11 Honey extract (Example 4) - - - 1.0 1.0 1.0 Hyaluronic acid 2.5 - - 1.5 - - Adenosine - 1.5 - - 0.5 - Arbutin - - 2.5 - - 1.5 Pro-type glycerin monostearate 2.0 2.0 2.0 2.0 2.0 2.0 Stearyl alcohol 2.2 2.2 2.2 2.2 2.2 2.2 Stearic acid 1.5 1.5 1.5 1.5 1.5 1.5 Wax 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 Sorbitan stearate 0.6 0.6 0.6 0.6 0.6 0.6 Purified vegetable oil 1.0 1.0 1.0 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 Mineral oil 5.0 5.0 5.0 5.0 5.0 5.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 Sodium magnesium silicate 0.1 0.1 0.1 0.1 0.1 0.1 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 Betaine 3.0 3.0 3.0 3.0 3.0 3.0 Triethanolamine 1.0 1.0 1.0 1.0 1.0 1.0 Sodium hyaruronate 4.0 4.0 4.0 4.0 4.0 4.0 Preservative, fragrance, pigment a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance

Test Example 13: Effect of improving skin moisturizing activity

Skin moisturization was evaluated by measuring the skin conductivity using a skin moisture meter (Corneometer, Courage + Khazaka, GmbH, Germany) as a clinical test for skin moisturization. The skin moisture was measured by subjecting the cosmetic compositions of Example 9 and Comparative Example 1 to half of the subject's face in half under constant temperature and humidity conditions maintained at a room temperature of 20 to 25 캜 and a relative humidity of 40 to 55% , The moisture content before and after 2 weeks was measured while applying the product of the comparative example (twice / day). The results are shown in Table 13 below.

division  The initial (D0) average 2 weeks (D14) Average Moisture Increase (%) Comparative Example 1 32 44 37 Example 9 31 47 52

As can be seen from the above Table 13, in Example 9 containing the enzyme-converted honey extract of the present invention and hyaluronic acid together, the moisture increase was higher than that of Comparative Example 1 containing only hyaluronic acid. Which is a moisturizing ingredient used together with the hyaluronic acid.

Test Example 14: Effect of enhancing skin wrinkle improving activity

In order to confirm the effect of improving the wrinkles of the skin, the formulations of Example 10 and Comparative Example 2 were applied for 10 weeks to each face for 2 weeks (morning and evening) for 4 weeks. After 4 weeks, the improvement of wrinkles was evaluated by image analysis of wrinkles.

The evaluation of the wrinkles by the image analysis was performed by taking replica under the eyes before the start of the experiment and collecting the replica immediately after the end of the experiment from the same area under the eyes and by analyzing the image by two dimensional analysis of the wrinkles, Were measured. The measurement results of the wrinkle density by the image analysis are shown in Table 14 below as a reduction rate relative to the wrinkle density before use.

division Wrinkle density reduction rate (%) Comparative Example 2 17 Example 10 29

As shown in Table 14, Example 10 containing the enzyme-converted honey extract of the present invention and adenosine together exhibited a higher wrinkle density reduction ratio than Comparative Example 2 containing only adenosine. When the honey extract of the present invention is used together with adenosine, which is an active ingredient for improving wrinkles, it can be seen that even with less adenosine, a useful component of the skin, it exhibits a more excellent wrinkle-reducing effect.

Test Example 15: Synergistic effect on skin tone improvement

In order to confirm the effect of improving the skin tone, the formulations of Example 11 and Comparative Example 3 were applied to the facial area twice a day (morning and evening) for 10 weeks, respectively, for 10 weeks. Prior to the start of the experiment, the tones of the skin to be measured were measured. After 4 weeks of application (twice / day), the tone improvement of the skin was measured using a colorimeter. L *, a *, b * colorimetric system is used to display color, and L * value (brightness) is used as an index in this experiment. The L * values are from black (L * = 0) to white (L * = 100), and ΔL * after 4 weeks is obtained. The results are summarized in Table 15 below.

division DELTA L * Comparative Example 3 8.42 Example 11 10.70

As shown in Table 15, it was confirmed that Example 11 containing the enzyme-converted honey extract of the present invention and arbutin together showed a better skin tone improving effect.

Claims (7)

A cosmetic composition comprising a honey extract prepared by treating a mixed enzyme of naringinase and pectinase in an amount of 0.01 to 15% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the mixed enzyme comprises naringinase and pectinase in a weight ratio of 1: 3: 1 to 3, respectively. The cosmetic composition according to claim 1, wherein the enzyme treatment is carried out under the conditions of a reaction pH of 4.5, a reaction temperature of 48 ° C, and a reaction time of 48 hours. [Claim 2] The cosmetic composition according to claim 1, wherein the honey extract exhibits an effect of enhancing the activity of a skin benefit component contained in the cosmetic composition. The cosmetic composition according to claim 4, wherein the skin benefit component is at least one selected from the group consisting of adenosine, arbutin and hyaluronic acid. [Claim 7] The cosmetic composition according to claim 1, wherein the cosmetic composition is used for skin exfoliation, skin moisturizing, whitening or skin wrinkle improvement. (A) extracting honey with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol;
(B) filtration, followed by concentration under reduced pressure and drying to prepare a honey extract powder;
(C) adding purified water to the honey solvent extract powder, adding a mixed enzyme comprising naringinase and pectinase in a weight ratio of 1 to 3: 1 to 3, respectively;
(D) stirring the enzyme reaction at pH 4.5 and 48 ° C for 48 hours with stirring; And
(E) sterilization, followed by concentration under reduced pressure and lyophilization.

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