KR102443283B1 - Cosmetic composition with excellent skin barrier using skin microbiome - Google Patents

Abstract

The present invention relates to a cosmetic composition having an excellent skin barrier improvement effect using a microbiome. In one embodiment, the cosmetic composition comprises a noni leaf extract ( Morinda Citrifolia Leaf Extract), a honey extract (Honey extract) and succinic acid (Succinic Acid), wherein the succinic acid contains glucose and Iodobacter sp. .) is obtained by fermentation using the strain.

Description

Cosmetic composition with excellent skin barrier improvement effect using microbiome {COSMETIC COMPOSITION WITH EXCELLENT SKIN BARRIER USING SKIN MICROBIOME}

The present invention relates to a cosmetic composition having an excellent skin barrier improvement effect using a microbiome. More particularly, the present invention relates to a cosmetic composition having excellent effects of adjusting the skin microbial balance, moisturizing the skin and improving the skin barrier using the microbiome.

Microbiome is a compound word of microbial community (microbiota) and genome (genome) and can be called 'microbiome genome'. means the whole In particular, the entire genetic information of microorganisms that exist in the human body or coexist in the human body is called the human microbiome and the human microbiome. Recently, research on the microbiome is being actively conducted as research on the interaction between humans and microorganisms has become more active, away from the existing research on individual microbiome analysis.

The skin is a complex ecosystem in which various microorganisms reside, and the correlation between the microbiome residing on the skin, the skin immune system, and the health of the skin is attracting attention. Beneficial bacteria and harmful bacteria coexist among the microbes residing on the skin, and the distribution of beneficial bacteria is greater in healthy skin, and the distribution of harmful bacteria is greater in atopic or acne-prone skin. In other words, maintaining the balance of microorganisms constituting the skin in a good state and creating a good environment is a fundamental approach that naturally improves skin immunity and creates a healthy skin barrier. Recently, the development of cosmetics to create a useful skin microbial community for the skin by appropriately controlling beneficial and harmful bacteria has also been reported.

The skin is largely divided into three layers: the epidermis, dermis, and subcutaneous tissue. The epidermis is divided into stratum corneum, clear layer, granular layer, stratum corneum, and basal layer from the outside to the inside. The stratum corneum, the outermost layer of the epidermis, is the most important structure of the skin barrier function. Consists of corneocytes and intercellular lipids. Various types of lipids such as sphingolipids, phospholipids, cholesterol sulfate, and neutral lipids exist between keratinocytes, and the function of the skin barrier to protect the skin from external stimuli do

The skin barrier plays a role in controlling transepidermal water loss (TEWL) in the stratum corneum and drug delivery through the skin. When the moisture of the normal keratinocyte layer is maintained at about 10% to 30%, the skin becomes smooth and soft, and the body's protective functions are normally exhibited. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost, which leads to aging. Peeling occurs because the moisture content of the keratinocyte layer is lower than that of normal skin.

However, even with healthy skin, the stratum corneum, which acts as a skin barrier, decreases moisture in the stratum corneum due to various causes such as various stresses, skin stress caused by environmental pollution, frequent washing, aging, etc. Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin and to strengthen the skin barrier.

A high concentration of natural moisturizing factor (NMF), a water-soluble component, exists in the cells constituting the stratum corneum, which helps the skin to show flexibility and maintain proper moisture. However, due to various stresses such as natural aging, makeup habits, and changes in lifestyle, the turn-over rate of the stratum corneum is slowed, the lipid synthesis ability of keratinocytes decreases, or normal cell division in the epidermis, As the maturation and differentiation are not smooth, the amount of moisturizing factors and lipids in the stratum corneum is reduced, and the number of people having skin in a state in which the normal stratum corneum function cannot be maintained, that is, the skin barrier function is broken, is increasing.

Due to the division and differentiation of these abnormal epidermal cells, the skin causes various skin diseases such as dryness, atopic dermatitis, and psoriasis. difficult. Therefore, strengthening the barrier that acts as a shield in the skin is a fundamental healing method.

On the other hand, acne refers to an inflammatory disease of the hair follicles, hair follicles and surrounding tissues due to the action of hormones, excessive sebum secretion, and bacterial infection.

There are several causes of acne, but the first cause is excessive secretion of sebum. Sebum is a substance secreted by the sebaceous glands, and in a normal amount, it prevents harmful substances from the outside of the skin from penetrating between the keratinocytes. However, under circumstances such as puberty, before and after menstruation, early pregnancy, taking contraceptives, and stress, the human body exhibits a different hormonal balance from the normal state. This changed hormonal balance stimulates the sebaceous glands, which leads to excessive secretion of sebum. In addition, there are cases of genetically high sebum secretion, and it can be increased by an increase in skin temperature such as external temperature and wrong eating habits.

The second cause is an infection of the pores. Due to the excessively secreted sebum, the PH of the skin surface increases, the bacteria causing acne easily proliferate on the surface of the skin with the increased PH, and the proliferated acne causes inflammation.

On the other hand, acne can be divided into 4 stages according to the symptoms. The first stage is comedonal acne, in which excessively secreted sebum accumulates in the pores around the sebaceous glands and forms comedones. Stage 1 comedonal acne is an early stage, mild acne lesion, but it is not good cosmetically and is a skin concern. Next is stage 2 papular acne. At this stage, acne bacteria proliferate and an inflammatory reaction occurs, resulting in red acne. In stage 3 purulent acne, the inflammatory reaction of red acne becomes more severe and a pocket of pus is formed inside, which is called a pustule. The last stage 4 nodular acne is a stage in which the pus bag bursts inside the skin and causes more inflammation around the acne affected area. can also be prolonged.

As a method for treating or improving acne, treatment of acne includes topical application therapy, oral drug administration, and surgical therapy such as laser treatment and dermabrasion. Although retinoids and corticosteroids are used for the purpose of inhibiting destruction, caution is required because these preparations have side effects such as skin rash and dry skin when overdose and may cause allergic reactions depending on the individual.

Cosmetics can be used for a long period of time compared to therapeutic drugs and can cause less damage to normal skin, so it is necessary to develop cosmetic raw materials that are safe for the skin and have excellent acne improvement effects from natural materials.

Considering the importance and function of the microbiome, which is emerging recently, creating an environment where beneficial microorganisms for the skin can be established through cosmetics establishes a physiologically useful physio-chemical balance for the skin. In addition to the skin barrier improvement effect, it is possible to control sebum and inhibit acne bacteria, improve acne and control sebum, and even improve the moisturizing effect.

Background art related to the present invention is disclosed in Republic of Korea Patent Publication No. 10-1798505 (2017.11.17. Announcement, title of invention: cosmetic composition for promoting beneficial microflora on the skin).

One object of the present invention is to provide a cosmetic composition excellent in the effect of improving the skin barrier function and adjusting the ratio of beneficial bacteria and harmful bacteria of the flora.

Another object of the present invention is to provide a cosmetic composition having excellent effects of improving skin troubles and acne, and controlling the amount of sebum.

Another object of the present invention is to provide a cosmetic composition that prevents skin irritation, has excellent formulation stability, and is harmless to the human body.

Another object of the present invention is to provide a cosmetic composition having excellent spreadability and excellent skin moisturizing effect when applied to the skin.

One aspect of the present invention relates to a cosmetic composition having an excellent skin barrier improvement effect using a microbiome. In one embodiment, the cosmetic composition comprises a noni leaf extract ( Morinda Citrifolia Leaf Extract), a honey extract (Honey extract) and succinic acid (Succinic Acid), wherein the succinic acid contains glucose and Iodobacter sp. .) is obtained by fermentation using the strain.

In one embodiment, the honey extract is obtained by fermentation extraction by inoculating honey with a strain, and the strain may include Zymomonas sp.

In one embodiment, the honey extract may be extracted using honey derived from at least one of Black Bee, Apis mellifera and Apis dorsata .

In one embodiment, the succinic acid is prepared by inoculating and fermenting a strain of Iodobacter sp. in a medium containing glucose; solvent extraction of the fermented product to prepare an extract; and heat-treating the extract.

In one embodiment, the noni leaf extract and honey extract may be included in a weight ratio of 1:0.2 to 1:5.

In one embodiment, the cosmetic composition is a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray formulation. can

In one embodiment, the cosmetic composition may be an Oil-in-Water (O/W), Water-in-Oil (W/O) or Water-in-Silicone (W/S) formulation.

The cosmetic composition according to the present invention is excellent in the effect of improving the skin barrier function and adjusting the ratio of beneficial and harmful bacteria of the flora , It has excellent formulation stability and may be harmless to the human body.

1 is a graph showing changes in transdermal water loss (TEWL) according to Example 1. FIG.
2 is a graph showing changes in the amount of transdermal water loss (TEWL) according to the use of Examples 1 to 3 and Comparative Example 4.
3 is a graph showing changes in sebum production after 30 days of application of Examples 1 to 3 and Comparative Example 4 according to the present invention.
4 is a photograph showing changes in pore shrinkage and pore condition improvement according to the use of Example 1.
5 is a photograph showing the acne improvement effect according to the use of Example 1.
6 is a graph showing changes in the flora on the skin after 30 days of application of Example 1;

In describing the present invention, if it is determined that a detailed description of a related known technology or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted.

And the terms described below are terms defined in consideration of functions in the present invention, which may vary depending on the intention or custom of the user or operator, so the definition should be made based on the content throughout this specification describing the present invention.

Cosmetic composition with excellent skin barrier improvement effect using microbiome

One aspect of the present invention relates to a cosmetic composition having an excellent skin barrier improvement effect using a microbiome. In one embodiment, the cosmetic composition includes Morinda Citrifolia Leaf Extract, Honey extract and Succinic Acid.

Noni Leaf Extract

The Noni leaf extract can be identified and obtained from the leaf part of Noni ( Morinda citrifolia , Noni ). The noni is a plant having a therapeutic and nutritional aspect and has been reported in a wide and diverse range (Singh, Y., Ikahihifo, T., Panuve, M., Slatter, C., Folk medicine in Tonga. A study on the use of herbal medicines for obsteric and gynacological conditions and disoders. J. Ethnopharm 1984, 12, 305-325).

The origin of noni was moved from Southeast Asia to Australia, and then it was cultivated in Polynesia, the Caribbean region of India, and the northern and central regions of South America. Noni has been reported as a plant with various health benefits, such as antioxidant effects, anti-inflammatory effects, cancer, infectious diseases, asthma, diabetes, high blood pressure, and pain. In addition, the roots, stems, bark, leaves, flowers, and fruits of the noni plant have more than 40 different combinations as an herbal remedy (Bruggnecate, J.T., Native plants can heal your wounds. Honolulu Star-Bulletin Local News 1992, Feb. 2).

Noni contains glycosides, polysaccharides, iridoids, alkaloids, lignans, trisaccharide fatty acid esters, anthraquinones, scholpoletin ( scopoletin), morindin, vitamins, minerals, and the like.

In one embodiment, the noni leaf extract may be included in an amount of 0.001 to 3.0% by weight based on the total weight of the cosmetic composition. When included in the above range, the formulation stability of the cosmetic composition is excellent, there is no skin irritation, and the skin trouble improvement, skin barrier improvement and moisturizing effect can be excellent. For example, 0.001 to 1.0% by weight may be included.

honey extract

Honey is the nectar and pollen collected by bees from the flowers of various plants, converted and stored for their food. Honey contains two monosaccharides, namely glucose and fructose, and carbohydrates, minerals, vegetable pigments, enzymes and pollen. More than 80% of the components of the honey are monosaccharides such as glucose and fructose. The monosaccharide component has excellent hygroscopicity and forms a moisture film on the skin to have a continuous moisturizing effect.

The honey extract may be derived from at least one of Black Bee, Apis mellifera and Apis dorsata . For example, it may be derived from black bee. The Black Bee (European Dark Bees) is a bee that has evolved to survive in the extreme regions of the Swiss Alps since the Ice Age. Black bees live in Europe and there are about five types [European Dark Bees (German Dark Bees), Bumblebees, Carpenter Bees, Ground Bees, Western Honey bees], and compared to normal bees, they have a very dark, thick skin and long hair. It has strong vitality to adapt well to cold weather and strong UV rays, and it has the characteristics of surviving by adapting to the climate where it is difficult for normal bees to survive. When the black bee-derived honey extract is included, the moisturizing properties of the present invention may be excellent.

In one embodiment, the honey extract is obtained by fermentation extraction by inoculating honey with a strain, and the strain may include Zymomonas sp.

For example, it may include Zymomonas mobilis . The Zymomonas mobilis is an anaerobic (air-hating) bacterium that lives on glucose, fructose, and maltose as energy sources, and as a strong ethanol fermenting strain, it maintains fast sugar metabolism, and 2~2.6% of energy sources such as glucose are self-growth. For this purpose, the remainder is used for the production of ethanol and carbon dioxide (CO ₂ ). The Zymomonas mobilis is one of the types of bacteria that produce ethanol for making Pulque (alcoholic beverage made from fermented sap of plants) in the Aztec civilization for more than 1000 years. Only simple sugars such as sucrose, glucose, and fructose can be fermented, and complex polysaccharides are not fermented. This can be explained by helping the skin biome recovery after receiving external environmental stress. During fermentation extraction with the strain, the moisturizing and skin elasticity improvement effects of the honey extract may be more excellent.

For example, the honey extract can be obtained by inoculating 0.01 to 10 parts by weight of Zymomonas sp. strain in 100 parts by weight of honey, and fermenting it by a known method. When fermented under the above conditions, the moisturizing and skin elasticity improvement effects of the honey extract may be excellent.

In one embodiment, the honey extract may be included in an amount of 0.001 to 3.0% by weight based on the total weight of the cosmetic composition. When included in the above range, formulation stability may be excellent, and skin barrier improvement, moisturizing and sebum control effects may be excellent at the same time. For example, 0.001 to 1.0% by weight may be included.

In one embodiment, the cosmetic composition may include the noni leaf extract and the honey extract in a weight ratio of 1:0.2 to 1:5. When included in the above weight ratio, it is harmless to the human body and has excellent formulation stability, and the synergistic effect between the active ingredients is excellent, so that the skin barrier improvement and moisturizing effect are excellent, and the improvement effect of acne and skin troubles and the sebum control effect are excellent. can For example, it may be included in a weight ratio of 1:2 to 1:5.

succinic acid

The succinic acid is a dicarboxylic acid having the formula (CH ₂ ) ₂ (CO ₂ H) ₂ . The name of succinic acid is derived from the Latin succinum meaning "amber (fossil)", and it is also called succinic acid because it is thought to be the soul of the mineral amber since ancient times. In living things, succinic acid mainly exists as succinate in the form of anion, and succinate performs various biological roles, such as being involved in the reaction of conversion to fumaric acid by succinic acid dehydrogenase of the mitochondrial electron transport chain. Succinic acid produces ATP and also acts as a signaling molecule that reflects the metabolic state of the cell. Succinic acid is produced in the mitochondrial matrix through the citric acid cycle, an energy-generating process present in all living things. Succinic acid may exit the mitochondrial matrix and may be involved in alterations in gene expression patterns in the cytoplasm as well as in the extracellular space, in epigenetic regulation or in hormone-like signaling. As such, succinic acid links the regulation of cellular metabolism and cellular function.

In one embodiment, the succinic acid is obtained by fermenting glucose using a strain of Iodobacter sp. When succinic acid obtained by fermenting the glucose using an Iodobacter species strain is applied, moisturizing and skin barrier improvement effects may be excellent.

In one embodiment, the Iodobacter sp. strain may include one or more of Iodobacter arcticus and Iodobacter fluviatilli s.

In one embodiment, the succinic acid is prepared by inoculating a strain of Iodobacter sp. in a medium containing glucose and fermenting (culturing) to prepare a fermentation (culture); solvent extraction of the fermentation (culture) product to prepare an extract; and heat-treating the extract.

The fermentation can be prepared by inoculating a strain of Iodbacter sp. in a TSA (Tryptic soy agar) medium containing glucose and fermenting it at 25-30° C. for 16 hours to 24 hours. Fermented products can be easily prepared during fermentation under the above conditions.

In one embodiment, the TSA medium may contain 0.1-20 g/L glucose, 5-20 g/L peptone, and 1-8 g/L tryptone. For example, the TSA medium may contain 0.1-20 g/L of glucose, 15 g/L of peptone and 5/L of tryptone. When the cultured and obtained succinic acid using the medium is applied, moisturizing and skin barrier improvement effects may be excellent.

In addition, after the fermentation, inactivation may be performed by a known method and then solvent extraction may be performed.

In one embodiment, in the solvent extraction, the fermented product may be extracted using alcohol. Under the above conditions, useful components contained in the fermented product can be easily extracted. For example, it can be extracted using ethanol.

In one embodiment, the heat treatment may heat-treat the fermented product at 100 to 125° C. for 1 to 60 seconds. Under the above conditions, the sterilization effect may be excellent.

In one embodiment, the succinic acid is prepared by filtering the heat-treated fermented product at 25-35° C. for 20-48 hours and then filtering; And drying the fluidized bed of the filtrate; may further include.

In one embodiment, the succinic acid may be included in an amount of 0.0001 to 0.5% by weight based on the total weight of the cosmetic composition. When included in the above range, formulation stability, moisturizing and barrier improvement effects may be excellent at the same time.

In one embodiment, the cosmetic composition may include the succinic acid, honey extract, and noni leaf extract in a weight ratio of 1:5-50:10-50. When included in the above weight ratio, it is harmless to the human body and has excellent formulation stability, and has excellent unpredictable synergistic effects among the active ingredients, so it has excellent skin barrier improvement and moisturizing effect, and improvement of acne and skin troubles and sebum control effect. can be excellent For example, it may be included in a weight ratio of 1:5-25:15-50.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. have.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/ propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, fatty acid esters of 3-butylene glycol, glycerol fatty esters, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

For example, the cosmetic composition of the present invention includes polysorbate 60, sorbitan sesquioleate, stearic acid, cetyl alcohol, squalane, glycerin, butylene glycol, arginine, carbomer, tocopheryl acetate, propanediol, 1, At least one of 2-hexanediol and purified water may be further included.

In addition, the cosmetic composition may include a surfactant, a moisturizer, a vegetable oil, an ester oil, a sunscreen, an antioxidant, a pH adjuster, a pigment, a fragrance, a preservative, a thickener, an emulsifier, a solubilizer, a plant extract, and the like.

The cosmetic composition may be an Oil-in-Water (O/W), Water-in-Oil (W/O) or Water-in-Silicone (W/S) formulation.

The cosmetic composition according to the present invention is excellent in the effect of improving the skin barrier function and adjusting the ratio of beneficial and harmful bacteria of the flora , It has excellent formulation stability and may be harmless to the human body.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, these are presented as preferred examples of the present invention and cannot be construed as limiting the present invention in any sense. Content not described here will be omitted because it can be technically inferred sufficiently by those skilled in the art.

Examples and Comparative Examples

By applying the ingredients and contents described in Table 1 below, a cosmetic composition of a cream formulation was prepared in a conventional manner.

(A) Noni leaf extract: It was obtained by isolating and identifying major compounds through extraction and fractionation from noni leaves.

(B) Honey extract: Zymomonas mobilis strain was inoculated into honey derived from black bee, and fermented by a known method to prepare a honey extract.

(C) Succinic acid: Iodobacter sp. As a strain, Iodobacter arcticus or Iodobacter fluviatilli s) was prepared.

Then, the Iodobacter species strain is inoculated into a TSA medium containing glucose 0.1-20 g/L, 15 g/L peptone and 5/L tryptone, and fermented at 25-30° C. for 16-24 hours to ferment. Water was prepared. After inactivation of the fermented product, the extract was prepared by solvent extraction with ethanol. The extract is heat-treated at 100-125° C. for 1-60 seconds, then further aged at 25-35° C. for 20-48 hours, followed by filtering to prepare a filtrate; Then, the fluidized bed of the filtrate was dried to obtain succinic acid.

Preparation of cosmetic composition: By applying the ingredients and contents described in Table 1 below, a cosmetic composition of a cream formulation was prepared in a conventional manner.

Test Example 1: Evaluation of skin irritation

When the cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 to 4 were used, a skin patch test was performed to investigate the skin irritation test. Subjects were selected as 25-55 year olds without skin disease at the test site, and a total of 20 subjects were assigned to 10 groups. After wiping the upper arm, which is the patch site, with 70% ethanol and drying it, 0.1 g of the cosmetic compositions of Examples and Comparative Examples were added and patched for 24 hours. After 48 hours from the start of the patch), it was tested again. The skin reaction was determined according to the following test criteria. The skin patch test results are shown in Table 2 below.

* Skin reaction test standard

- No reaction ± Weak positive reaction (erythema) + Positive reaction (erythema) ++ Strong positive reaction (erythema, edema) +++ Severe positive reaction (erythema, edema, blisters)

Referring to the results of Table 2, in the case of the cosmetic compositions of Examples 1-3 of the present invention, it was found that the skin irritation was less than that of Comparative Examples 1-4.

Test Example 2: Formulation stability evaluation

In order to measure the stability with respect to the degree of separation and discoloration for the cosmetics of Examples 1 to 3 and Comparative Examples 1 to 4, a thermostat maintained at 45° C. and 25° C., constantly maintained at 4° C., and a refrigerator and daylight conditions As a result, the composition of Examples and Comparative Examples was placed in a sample container and stored for 4 weeks, and the degree of separation and discoloration was compared and measured, and the results are shown in Table 3 below. At this time, the degree of separation and discoloration of the product was evaluated by classifying it into the following 6 grades.

* Separation and discoloration evaluation criteria

0: No change 1: Slight separation (discoloration) 2: Slight separation (discoloration) 3: Slight separation (discoloration) 4: Severe separation (discoloration) 5: Extremely separation (discoloration)

Referring to the results of Table 3, it was found that Examples 1 to 3 of the present invention had less separation and discoloration compared to Comparative Examples 1 to 4, which was out of the configuration of the present invention, and thus had excellent formulation stability.

Experimental Example 3: Measurement and evaluation of TEWL change in transdermal water loss

For the cosmetic compositions of Examples and Comparative Examples, the amount of change in TEWL (Transepidermal water loss) was measured for Examples 1 to 3 and Comparative Example 4. Through the change in the amount of transdermal water loss in the Examples and Comparative Examples, the change in skin moisture retention and skin barrier function of the cosmetic composition of the present invention was evaluated.

Specifically, the experiment was conducted with 23 women in the clinical group. 23 subjects participated in the evaluation experiment, and the skin conditions of 23 subjects who completed the test were dry skin 4 people, medium dry skin 5 people, neutral skin 3 people, oily skin 6 people, and oily skin 5 people. In the questionnaire about skin disease, itchiness, stinging, erythema, cosmetic side effects, drug side effects, photosensitivity, and atopic disease, there were no subjects who were eligible, and there were no subjects who had experience with other items.

In addition, for the instrumental evaluation, the test subjects were allowed to adjust the skin surface temperature and humidity to the environment of the measurement space by resting for 30 minutes in a waiting room under constant temperature and humidity conditions with an indoor temperature of 20~25℃ and a humidity of 40~60%. Water intake was restricted while taking

Transdermal water loss (TEWL) was measured using a Tewameter, TEWL immediately after washing immediately after using Examples and Comparative Examples for 15 days, and 1 day and 2 days after washing the same inner part of the left arm. TEWL was measured and the results are shown in Table 4 and FIGS. 1 and 2 below.

1 is a graph showing the change in transdermal water loss (TEWL) according to Example 1, FIG. 2 is a cosmetic composition of Examples 1 to 3 and Comparative Example 4 according to the present invention was applied for 15 days, and then washed and applied It is a graph showing the change in skin TEWL after 1 day and 2 days after not doing it.

Referring to Table 4 and Figures 1 and 2, Examples 1 to 3 significantly reduced the amount of transdermal water loss immediately after washing for 15 days, 1 day and 2 days after use, compared to Comparative Example 4, Through this, it can be seen that the skin barrier improvement effect is excellent.

Experimental Example 4: Sebum production evaluation

For the cosmetic compositions of Examples and Comparative Examples, changes in sebum production were typically measured for Examples 1 to 3 and Comparative Example 4.

Specifically, the experiment was conducted on 20 female and male clinical groups between the ages of 12 and 30. There were 20 subjects who participated in the evaluation experiment, and the skin condition of the 20 subjects who completed the test was tested only with subjects with acne or oily skin. In the questionnaire about skin disease, itchiness, stinging, erythema, cosmetic side effects, drug side effects, photosensitivity, and atopic disease, there were no subjects who were eligible, and there were no subjects who had experience with other items.

In addition, for the instrumental evaluation, the test subjects were allowed to adjust the skin surface temperature and humidity to the environment of the measurement space by resting for 30 minutes in a waiting room under constant temperature and humidity conditions with an indoor temperature of 20~25℃ and a humidity of 40~60%. Water intake was restricted while taking

Sebumeter® (SM815) was used as a measuring device for the evaluation of the sebum production. Before and 30 days after use of the Examples and Comparative Examples, the same chin and forehead were measured and averaged, and the results are shown in Table 5 and FIG. 3 . The amount of sebum is measured in micrograms per cm ² of skin.

3 is a graph showing the change in sebum production 30 days after the application of Examples 1 to 3 and Comparative Example 4 according to the present invention. Referring to Table 5 and FIG. 3, Examples 1 to 3 significantly decreased (p<0.05) the sebum production (R2) value after 30 days of use, compared to Comparative Example 4, and through this, the reduction in skin sebum production was remarkable, It was found that the sebum control effect was excellent.

Experimental Example 5: Measurement of pore shrinkage change

For the cosmetic compositions of Examples and Comparative Examples, changes in pore size were typically measured for Examples 1 and 4, respectively.

Specifically, the experiment was conducted on 20 female and male clinical groups between the ages of 12 and 30. There were 20 subjects who participated in the evaluation experiment, and the skin condition of the 20 subjects who completed the test was tested only with subjects with acne or oily skin. In the questionnaire about skin disease, itchiness, stinging, erythema, cosmetic side effects, drug side effects, photosensitivity, and atopic disease, there were no subjects who were eligible, and there were no subjects who had experience with other items.

In addition, for the instrumental evaluation, the test subjects were allowed to adjust the skin surface temperature and humidity to the environment of the measurement space by resting for 30 minutes in a waiting room under constant temperature and humidity conditions with an indoor temperature of 20~25℃ and a humidity of 40~60%. Water intake was restricted while taking

For the evaluation of pore size, HD-VISIOFACE® (Microcaya) was used as a measuring device, and the amount of change in pore size was measured through close-up photography. Before and 30 days after use of the Examples and Comparative Examples, the same cheek area was measured and the average was calculated, and the results are shown in Table 6 and FIG. 4 below.

4 is a photograph showing changes in pore shrinkage and pore condition improvement according to the use of Example 1, and was taken for a tester whose pore change amount was noticeable when Example 1 was applied. Referring to the results of FIGS. 4 and 6, it was found that Examples 1 to 3 showed a significant (p < 0.05) decrease in pore size after 30 days of use compared to Comparative Example 4. The numerical values shown in Table 6, as the number is lower, it means that the overall area of the pores is reduced.

Experimental Example 6: Improvement in skin acne and troubles

For the cosmetic compositions of Examples and Comparative Examples, the degree of improvement of acne was measured representatively with respect to Example 1.

Specifically, the experiment was conducted on 20 female and male clinical groups between the ages of 12 and 30. There were 20 subjects who participated in the evaluation experiment, and the skin condition of the 20 subjects who completed the test was tested only with subjects with acne or oily skin. In the questionnaire about skin diseases, itchiness, stinging, erythema, cosmetic side effects, drug side effects, photosensitivity, and atopic disease, there were no subjects who were eligible, and there were no subjects who had experience with other items.

In addition, for the instrumental evaluation, the test subjects were allowed to adjust the skin surface temperature and humidity to the environment of the measurement space by resting for 30 minutes in a waiting room under constant temperature and humidity conditions with an indoor temperature of 20~25℃ and a humidity of 40~60%. Water intake was restricted while taking

To evaluate the amount of change in acne, HD-VISIOFACE® (Microcaya) was used as a measuring device, and it was taken through close-up photography. The same forehead and cheek areas were measured before and 30 days after use of Example 1, and the results are shown in FIG. 5 below.

5 is a photograph showing the acne improvement effect according to the use of Example 1. Referring to FIG. 5 , it was found that the skin acne improvement effect was excellent when Example 1 of the present invention was used.

Experimental Example 7: Degree of change of harmful bacteria and beneficial bacteria

For the cosmetic compositions of Examples and Comparative Examples, representatively, the amount of change in the flora on the skin was measured after 30 days of application on the skin for Example 1.

Specifically, the experiment was conducted for 30 days targeting 20 female and male clinical groups between the ages of 12 and 30. There were 20 subjects who participated in the evaluation experiment, and the skin condition of the 20 subjects who finished the test was tested only with subjects with acne or oily skin with a lot of sebum. In the questionnaire about skin diseases, itchiness, stinging, erythema, cosmetic side effects, drug side effects, photosensitivity, and atopic disease, there were no subjects who were eligible, and there were no subjects who had experience with other items.

In addition, for the instrumental evaluation, the test subjects were allowed to adjust the skin surface temperature and humidity to the environment of the measurement space by resting for 30 minutes in a waiting room under constant temperature and humidity conditions with an indoor temperature of 20~25℃ and a humidity of 40~60%. Water intake was restricted while taking

The change in the bacterial distribution was compared to the relative bacterial distribution before and 30 days after application of Example 1 by identification and analysis after peeling off the flora on the skin through tape stripping, and the results are shown in FIG. 6 .

As an evaluation of changes in bacterial distribution, Staphylococcus epidermis , Propionibacterium acnes , and Staphylococcus aureus 3 species were compared. Among them, the Staphylococcus aureus and Propionibacterium acnes correspond to harmful bacteria, and Staphylococcus epidermis corresponds to beneficial bacteria.

6 is a graph showing changes in the flora on the skin after 30 days of application of Example 1; Referring to the results of FIG. 6, when Example 1 of the present invention was applied, changes in the skin flora were induced, the beneficial bacteria increased, and the harmful bacteria decreased. By increasing the dominance of , it was confirmed that the skin microbial balance adjustment and skin environment improvement effect were excellent.

Experimental Example 8: Effect of use

With 10 female and male clinical groups between 12 and 30 years old as one group, the Examples 1 to 3 and Comparative Examples 1 to 4 test products were respectively applied to a total of 7 groups for 4 weeks, and then each group A questionnaire was conducted on the improvement of the skin barrier, moisturizing, pore shrinkage, skin troubles, and the spreadability of cosmetics for the experimental participants. At this time, the case in which the use effect of the test product was the most excellent was taken out of 5 points, and the average thereof is shown in Table 7 below.

Referring to the results of Table 7, Examples 1 to 3 of the present invention, compared to Comparative Examples 1 to 4, the skin barrier improvement, moisturizing, pore reduction and skin trouble improvement effects were excellent, and it was found that the spreadability was also excellent.

Up to now, the present invention has been mainly examined in the examples. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (8)

It contains Succinic Acid, Honey extract, and Morinda Citrifolia Leaf Extract in a weight ratio of 1:5-50:10-50.
The succinic acid is a cosmetic composition, characterized in that it is obtained by fermenting glucose using Iodobacter sp. strain.
delete According to claim 1, wherein the honey extract is obtained by fermentation extraction by inoculating honey with a strain,
The strain is a cosmetic composition comprising the genus Zymomonas ( Zymomonas sp.).
The cosmetic composition according to claim 1, wherein the honey extract is extracted using honey derived from at least one of Black Bee, Apis mellifera , and Apis dorsata .
The method of claim 1 , wherein the succinic acid comprises: inoculating and fermenting a strain of Iodobacter sp. in a medium containing glucose to prepare a fermented product;
solvent extraction of the fermented product to prepare an extract; and
A cosmetic composition, characterized in that it is obtained including; heat-treating the extract.
delete According to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray Cosmetic composition, characterized in that the formulation.
The cosmetic composition according to claim 1, wherein the cosmetic composition is an Oil-in-Water (O/W), Water-in-Oil (W/O), or Water-in-Silicone (W/S) formulation. .

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