KR102547786B1 - Cosmetic composition having excellent anti oxidation with improving skin tone - Google Patents

Abstract

The present invention relates to a cosmetic composition with excellent antioxidant and complexion improvement effects. In one embodiment, the cosmetic composition includes Swiss glacial water and succinic acid derived from an Iodobacter ssp. strain as active ingredients.

Description

Cosmetic composition with excellent antioxidant and complexion improvement {COSMETIC COMPOSITION HAVING EXCELLENT ANTI OXIDATION WITH IMPROVING SKIN TONE}

The present invention relates to a cosmetic composition with excellent antioxidant and complexion improvement effects.

Since the skin is one of the organs constituting the human body, mental stress, lack of sleep, and external stimuli accepted by the human body gradually or immediately appear on the skin.

Mental and physical stress disrupts the sympathetic nervous system and overproduces active oxygen. When stress is severe or prolonged, the sympathetic nervous system and parasympathetic nervous system are out of balance because of the adrenaline that is explosively secreted from the sympathetic nervous system. Adrenaline is a hormone that is essential for human survival and helps us overcome the crisis in front of our eyes.

In addition, the moment adrenaline is secreted, active oxygen is created the most in our body. Active oxygen is produced a lot when the flow of blood vessels suddenly increases. In other words, when adrenaline is secreted and the flow of blood gathered to the heart suddenly increases, active oxygen also increases rapidly. Free radicals lead to skin cell and tissue damage. In addition, they disrupt the antioxidant defense system in the body, tipping the balance between oxidants and antioxidants toward the oxidation state. Free radicals lead to skin cell and tissue damage. Continued oxidative stress leads to lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy intercellular lipid components, chain scission and abnormal cross-linking of collagen and elastin, which are elastic fibers, severing of hyaluronic acid chains, promotion of melanin production, and DNA It causes damage to biological components such as oxidation. In addition, it causes inflammation in the skin and suppresses skin immune function, resulting in an increase in bacterial infection or carcinogenesis. As a result, skin aging characterized by loss of elasticity, wrinkles and melasma, and freckles is accelerated (Source: Park Soo-nam, Skin Aging and Active Oxygen, Seoul National University of Technology, 50; p.329-341, 1999).

The endoplasmic reticulum (ER) is an important organelle responsible for protein and lipid synthesis, calcium storage, and signal transduction. Cells produce proteins to perform their respective functions, which are selectively and precisely regulated according to environmental changes. The endoplasmic reticulum is an organelle responsible for "quality control" of proteins, and proteins are transported to the appropriate location after undergoing structural formation and modification. Endoplasmic reticulum stress (ER stress), in which unfolded proteins increase in the endoplasmic reticulum, is caused by various causes that change the protein homeostasis network, one of which is oxidative stress (source: material line, endoplasmic reticulum stress and unfolded protein reaction) Research Trends, Molecular Cell Biology Newsletter).

Proteins form unique structures through protein folding, which is directly related to protein functions, and protein misfolding prevents proteins from forming their own three-dimensional structure, leading to cell death. When a protein with an incorrect structure is formed due to misfolding, it is restored to the correct structure by factors called chaperones or degraded by proteolytic enzymes. However, some proteins are not removed even though they have the wrong structure and form protein aggregates. Misfolded proteins randomly combine with each other and can cause various diseases, including cancer and cardiovascular disease.

For this reason, in the case of modern people who are exposed to many active oxygen increasing factors such as ultraviolet rays and mental stress, skin cells are easily damaged by protein folding and various oxidative stresses, and various skin concerns such as loss of elasticity, wrinkles, and dryness are accompanied. Therefore, in order to delay or prevent skin aging, it is necessary to establish an antioxidant defense system that suppresses excessive production of active oxygen, removes the generated active oxygen, and helps normal expression of proteins to maintain normal skin function.

Cosmetics are the best tool to prevent skin troubles and solve problems by applying them consistently every day. However, no matter how good the ingredients are, there is a limit to the absorption of active ingredients by the in vivo barrier system of the skin. Therefore, daily use of cosmetics with increased absorption by combining good ingredients with delivery technology for better absorption into the skin can make the skin structure that is easily broken down by external stimuli healthy. In particular, liposome technology is composed of a lipid bilayer that is structurally similar to intercellular lipids in the cell membrane or stratum corneum, and is a formulation that fuses with the cell membrane to effectively deliver active ingredients into cells. Research Trends, Kor.J.Aesthet.Cosmetol., Vol.12 No.5, 597-605, October 2014).

One object of the present invention is to provide a cosmetic composition excellent in preventing skin aging from thermal stress and oxidative stress.

Another object of the present invention is to provide a cosmetic composition excellent in preventing oxidative damage to skin fibroblasts and promoting recovery of damaged fibroblasts.

Another object of the present invention is to provide a cosmetic composition with excellent skin permeability, formulation stability and storage properties.

Another object of the present invention is to provide a cosmetic composition with excellent application properties and a feeling of use.

Another object of the present invention is to provide a cosmetic composition with an excellent effect of helping normal protein folding so that protein function can maintain homeostasis in damaged human epidermal cells.

Another object of the present invention is to provide a cosmetic composition excellent in skin recovery and damage prevention effects from various external stress environments such as lack of sleep.

Another object of the present invention is to provide a cosmetic composition that is excellent in reducing fine wrinkles and has an excellent effect in improving complexion by restoring skin radiance.

Another object of the present invention is to provide a cosmetic composition excellent in improving skin antioxidant activity and maintaining skin moisturizing effect.

One aspect of the present invention relates to a cosmetic composition with excellent antioxidant and complexion improvement effects. In one embodiment, the cosmetic composition includes Swiss glacial water and succinic acid derived from an Iodobacter ssp. strain as active ingredients.

In one embodiment, the Iodobacter species strain may be obtained from soil in Valais, Switzerland.

In one embodiment, based on the total weight of the cosmetic composition, 0.0001 to 10.0% by weight of the Swiss ice water and 0.00001 to 0.05% by weight of succinic acid may be included.

In one embodiment, the cosmetic composition may further include a phospholipid compound.

In one embodiment, the cosmetic composition may have a skin protecting effect from skin thermal damage and oxidative stress.

In one embodiment, the cosmetic composition may have an effect of improving skin complexion by improving skin wrinkles and roughness.

In one embodiment, the cosmetic composition may have external moisturizing and moisturizing effects on the skin.

In one embodiment, the cosmetic composition may be a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax or spray formulation there is.

In one embodiment, the cosmetic composition is Oil-in-Water (water-in-water type) (O / W), Water-in-Oil (water-in-oil type) (W / O), Water-in-Silicone (silicone-in-water type) (W /S) or Water-less.

The cosmetic composition according to the present invention has excellent skin aging prevention effect from heat stress and oxidative stress, excellent skin permeability, formulation stability and storage stability, excellent application and feeling of use, and an effect of preventing oxidative damage to skin fibroblasts and It has an excellent effect of promoting recovery of damaged fibroblasts, and an excellent effect of helping normal folding of proteins so that protein function can maintain homeostasis in damaged human epidermal cells. It has an excellent prevention effect, an excellent effect of alleviating fine wrinkles, an excellent effect of improving complexion by restoring skin radiance, and an excellent effect of improving skin antioxidant capacity and maintaining skin moisturizing effect.

1 is a graph showing the increase in cell viability after heat stress treatment of human epidermal cells treated with a mixture of succinic acid and Swiss ice water according to the present invention.
Figure 2 is a graph showing the chaperone expression level of senescent epidermal cells treated with a mixture of succinic acid and Swiss ice water according to the present invention.
Figure 3 is a graph comparing the cellular ATP production rate of human keratinocytes treated with the mixture according to the present invention.
Figure 4 is a graph comparing the effect of reducing ER Stress after treating human epidermal cells with the mixture of the present invention.
Figure 5 is a fluorescence micrograph comparing the effect of reducing ER Stress after treating human epidermal cells with the mixture of the present invention.
Figure 6 is a graph measuring the change in maximum wrinkle depth upon treatment with the mixture of the present invention.
7 is a photograph showing the effect of improving skin wrinkles and complexion when the mixture of the present invention is treated.
8 is a graph showing the effect of improving skin radiance when the mixture of the present invention is treated.
9 is a graph comparing the effect of improving fine wrinkles (glabella) when Example 1 was applied.
10 is a graph comparing the effect of improving fine wrinkles (around the eyes) when Example 1 was applied.
11 is a graph comparing the effect of improving fine wrinkles (nasolabial folds) when Example 1 is applied.
12 is a photograph showing the effect of improving fine wrinkles (glabella, around the eyes, nasolabial folds) when Example 1 was applied.
13 is a graph comparing the effect of improving elasticity in the skin when Example 1 is applied.
14 is a graph comparing the effect of improving skin radiance (gloss) when Example 1 is applied.
15 is a graph comparing the skin surface moisturizing improvement effect when Example 1 is applied.
16 is a graph comparing the moisturizing improvement effect in the skin (2.5 mm) when Example 1 is applied.
17 is a graph showing the skin texture (roughness) measurement results of Example 1.
18 is a photograph showing the skin texture improvement effect of Example 1.

In describing the present invention, if it is determined that a detailed description of a related known technology or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

In addition, the terms to be described below are terms defined in consideration of functions in the present invention, which may vary according to the intention or custom of the user or operator, so the definitions should be made based on the content throughout this specification describing the present invention.

Cosmetic composition with excellent antioxidant and complexion improvement effects

One aspect of the present invention relates to a cosmetic composition with excellent antioxidant and complexion improvement effects. In one embodiment, the cosmetic composition includes Swiss glacial water and succinic acid derived from an Iodobacter ssp. strain as active ingredients.

swiss glacial water

When snow accumulates over tens of millions of years without melting in high mountains or polar regions to form ice caps, it is turned into solid ice by pressure, and the water produced from the ice is called glacial water. The glacier water used in the present invention is melted into water itself and passed through natural filtering devices such as granite, gravel, and fine particles, and the filtered glacial water naturally ejected to the ground can be collected. That is, the glacial water of the present invention may be obtained through natural filtration in which an aquifer located deep underground is not polluted by any material by the stratum, which is a natural protection device, and may be in a pre-filtered state.

In one embodiment, glacial water collected in Valais, Switzerland may be used as the glacial water. When glacial water is included in the cosmetic composition in the above region, the skin moisturizing effect may be excellent.

In one embodiment, the ice water may be included in an amount of 0.0001 to 10.0% by weight based on the total weight of the cosmetic composition. When included in the content range, the formulation stability, skin application and feeling of use of the cosmetic composition are excellent, and the effect of increasing the moisture content of the skin epidermal layer and the dermis layer without skin irritation, improving skin moisture density, and skin moisturizing effect can be excellent. For example, the ice water is 0.0001 to 6.0% by weight, 0.0005 to 5.0% by weight, 0.001 to 3.0% by weight, 0.005 to 3.0% by weight, 0.05 to 3.0% by weight, 0.1 to 2.0% by weight or 0.5% by weight relative to the total weight of the cosmetic composition. ~2.0% by weight.

Succinic Acid

The succinic acid is a dicarboxylic acid with the formula (CH ₂ ) ₂ (CO ₂ H) ₂ . The name of succinic acid is derived from the Latin word succinum, which means "amber (fossil)", and is also called amber acid because it has been thought to be the soul of the mineral amber since ancient times. In living organisms, succinic acid mainly exists in the form of an anion, succinate, and succinate plays various biological roles, such as being involved in a reaction in which succinic acid is converted to fumaric acid by succinic acid dehydrogenase in the mitochondrial electron transport system. Succinic acid generates ATP and acts as a signaling molecule that reflects the metabolic state of cells. Succinic acid is produced in the mitochondrial matrix through the citric acid cycle, an energy-producing process present in all living things. Succinic acid may exit the mitochondrial matrix and be involved in changes in gene expression patterns, regulation of epigenetics, or hormone-like signaling in the extracellular space as well as the cytoplasm. As such, succinic acid links cellular metabolism with the regulation of cellular functions.

In one embodiment, the Iodobacter species strain may be obtained from soil in Valais, Switzerland. When using the Iodobacter strain collected from the soil of the above region, when succinic acid is applied, the effect of moisturizing and improving the skin barrier may be excellent.

For example, the succinic acid can be obtained by fermentation in a medium containing glucose using an Iodobacter spp. strain collected from soil covered by glaciers in Valais, Switzerland. When succinic acid obtained by fermentation using an Iodobacter sp. strain is applied to the glucose-containing medium, moisturizing and skin barrier improvement effects may be excellent.

In one embodiment, the strain of Iodobacter spp. may include at least one of Iodobacter arcticus and Iodobacter fluviatillis. When succinic acid obtained by fermentation using the above strain is applied, antioxidation, skin complexion improvement, skin moisturizing, and skin barrier improvement effects may be excellent.

In one embodiment, the succinic acid is inoculated with an Iodobacter spp. strain in a medium containing glucose and fermented (cultured) to prepare a fermentation (culture) product; Preparing an extract by solvent extraction of the fermentation (culture) product; It may be prepared including; and heat-treating the extract.

The fermentation is performed by inoculating Iodobacter spp. strains collected from soil covered with glaciers in the Valais region of Switzerland in a TSA (Tryptic soy agar) medium containing glucose and inoculating the strain at 25 to 30 ° C. for 16 to 24 hours It can be fermented to produce a fermented product. When fermented under the above conditions, a fermented product can be easily produced.

In one embodiment, the TSA medium may include 0.1 to 20 g/L glucose, 5 to 20 g/L peptone, and 1 to 8 g/L tryptone. For example, the TSA medium may include 0.1 to 20 g/L glucose, 15 g/L peptone, and 5/L tryptone. When applying the cultured and obtained succinic acid using the above medium, moisturizing and skin barrier improvement effects may be excellent.

In one embodiment, the fermentation may be carried out one or more times. For example, the first fermented product is fermented at 25 to 30 ° C for 16 to 24 hours, and the first fermented product is fermented at 25 to 30 ° C for 16 to 24 hours for secondary fermentation. Thus, a secondary fermented product can be prepared. Under the above conditions, moisturizing and skin barrier improvement effects may be excellent. In addition, after the fermentation, inactivation may be performed by a known method, followed by solvent extraction.

In one embodiment, the solvent extraction may extract the fermented product using alcohol. Under these conditions, useful components contained in the fermented product can be easily extracted. For example, it can be extracted using ethanol.

In one embodiment, the heat treatment may heat-treat the fermented product at 100 to 125 ° C. for 1 to 60 seconds. Under the above conditions, the sterilization effect may be excellent.

In one embodiment, the succinic acid is filtered after additional aging of the heat-treated fermented product at 25 to 35 ° C. for 20 to 48 hours to prepare a filtrate; And drying the fluidized layer of the filtrate; may further include.

In one embodiment, the succinic acid may be included in an amount of 0.00001 to 0.5% by weight based on the total weight of the cosmetic composition. When included in the above range, formulation stability, skin moisturizing, antioxidant and barrier improvement effects may be excellent at the same time. For example, the succinic acid is present in an amount of 0.00005 to 0.5% by weight, 0.0001 to 0.5% by weight, 0.0005 to 0.5% by weight, 0.001 to 0.5% by weight, 0.001 to 0.4% by weight, or 0.001 to 0.2% by weight based on the total weight of the cosmetic composition. , 0.001 to 0.1% by weight, 0.001 to 0.05% by weight or 0.001 to 0.01% by weight may be included.

In one embodiment, the cosmetic composition may include the succinic acid and Swiss ice water in a weight ratio of 1:200 to 1:800. When included in the above weight ratio range, the cosmetic composition may have excellent mixability and formulation stability, and may have excellent skin moisture density improvement, skin moisturizing, skin barrier improvement, and skin complexion improvement effects without skin irritation. For example, 1:250 to 1:800 weight ratio, 1:300 to 1:800 weight ratio, 1:450 to 1:700 weight ratio, 1:550 to 1:650 weight ratio, or 1:550 to 1:600 weight ratio may be included. there is.

phospholipid compounds

In one embodiment, the cosmetic composition may further include a phospholipid compound. When the phospholipid compound is included, it is possible to easily form a liposome by easily supporting the active ingredient of the cosmetic composition.

On the other hand, in the present invention, glacial water and succinic acid in the form of a mixture (complex) as active ingredients can be captured and used in liposomes in order to be easily used as raw materials for cosmetic compositions. When the liposome formulation is applied, it is possible to simultaneously stabilize the active ingredient in the cosmetic composition to improve formulation stability and percutaneous absorption.

In one embodiment, the cosmetic composition may include liposomes prepared by applying any drug delivery system (DDS) technology as long as it does not affect the formulation, including the liposome method commonly used in the art.

The present invention applied a cosmetic composition in the form of a liposome in consideration of the fact that the gap between lipids between cells in the stratum corneum is about 50 nm and that emulsified particles such as liposomes have excellent affinity with skin lipids. When the liposome is applied, absorption into intercellular lipids can be improved.

In one embodiment, the phospholipid compound may include at least one of a lecithin compound, a cephalin compound, and a sphingomyelin compound. For example, the phospholipid may include a lecithin compound.

The lecithin compound has amphiphathic properties and can easily form liposomes when the lecithin is included. In one embodiment, the lecithin compound may include at least one of soybean lecithin, egg yolk lecithin, hydrogenated lecithin, hydrogenated lysolecithin, and hydroxylated lecithin.

In one embodiment, the phospholipid compound may be included in an amount greater than 0 and 3.0% by weight or less based on the total weight of the cosmetic composition. When included under the above conditions, the formulation stability is excellent by easily supporting the active ingredient, and the skin transdermal penetration effect may be excellent. For example, the phospholipid compound may be included in an amount of 0.001 to 3.0 wt%, 0.001 to 2.5 wt%, 0.001 to 2.0 wt%, 0.01 to 1.0 wt%, or 0.01 to 0.2 wt% based on the total weight of the cosmetic composition.

maltodextrin

In one embodiment, the cosmetic composition may further include maltodextrin. The maltodextrin may be included to improve transdermal penetration efficiency while improving elasticity and durability of the phospholipid layer.

In one embodiment, the maltodextrin may be included in an amount greater than 0 and less than 2.0% by weight based on the total weight of the cosmetic composition. When included under the above conditions, the formulation stability is excellent by easily supporting the active ingredient, the elasticity and durability of the phospholipid layer are formed, and the skin transdermal penetration effect may be excellent. For example, the maltodextrin may be included in an amount of 0.0001 to 2.0% by weight, 0.005 to 1.5% by weight, 0.01 to 1.0% by weight, 0.1 to 1.0% by weight, or 0.3 to 1.0% by weight based on the total weight of the cosmetic composition.

In one embodiment, the cosmetic composition includes a liposome, but the liposome is a phospholipid layer; and a carrier supported inside the phospholipid layer and containing glacial water and succinic acid.

In one embodiment, the liposome may include the carrier and the phospholipid layer in a weight ratio of 1:1 to 1:30. When included in the weight ratio range, the formulation stability of the cosmetic composition is excellent, the durability of the liposome is excellent, and the penetration efficiency and economy of active ingredients into the skin may be excellent. For example, the liposome contains the carrier and the phospholipid layer at a weight ratio of 1:2 to 1:30, 1:3 to 1:25, 1:5 to 1:20, or 1:10 to 1:20. can be included with

In one embodiment, the phospholipid layer may include a lecithin compound and maltodextrin. In one embodiment, the phospholipid layer may include a lecithin compound and maltodextrin in a weight ratio of 1:6 to 1:50. Under the above conditions, the cosmetic composition may have excellent mixability and formulation stability, excellent elasticity and durability of the phospholipid layer, and excellent skin transdermal penetration effect. For example, the phospholipid layer contains a lecithin compound and maltodextrin at a weight ratio of 1:10 to 1:45, 1:15 to 1:40, 1:20 to 1:40, or 1:25 to 1:35. can be included with

In one embodiment, the cosmetic composition may include 0.001 to 70% by weight of the liposome. When included in the above content range, formulation stability is excellent and skin moisturizing, antioxidant, and skin complexion improvement effects may be excellent without skin irritation. For example, 0.001 to 60% by weight, 0.001 to 50% by weight, 0.005 to 40% by weight, 0.01 to 30% by weight, 0.05 to 20% by weight or 0.1 to 15% by weight may be included.

The cosmetic composition may have an excellent effect of activating ATP generation and reducing ER stress in skin cells in which endoplasmic reticulum (ER) stress is increased due to lack of sleep.

The cosmetic composition may have an excellent effect of improving the expression rate of ER chaperon, and thus may have an excellent effect of maintaining protein function homeostasis in aged cells.

In one embodiment, the cosmetic composition may have a skin protecting effect from skin thermal damage and oxidative stress. For example, the cosmetic composition can prevent skin aging due to heat stress on the skin.

In one embodiment, the cosmetic composition may have an effect of improving skin complexion by improving skin wrinkles and roughness.

In one embodiment, the cosmetic composition may have external moisturizing and moisturizing effects on the skin.

The cosmetic composition according to the present invention is not particularly limited in its formulation. For example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, soap, solid cream, pack, powder, body lotion, body cream It can be formulated into body oil, body essence, makeup base, foundation, hair dye, shampoo, rinse, body cleanser, toothpaste and mouthwash, and can be formulated into skin whitening cosmetics or medicines such as ointments and patches. there is.

In one embodiment, the cosmetic composition may be a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax or spray formulation there is.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. there is.

When the formulation of the present invention is a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane/butane or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, fatty acid esters of glycerol, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

For example, the cosmetic composition of the present invention includes sorbitan sesquioleate, stearic acid, cetyl alcohol, squalane, glycerin, butylene glycol, arginine, carbomer, tocopheryl acetate, propanediol, 1,2-hexanediol and One or more of purified water may be further included.

According to an embodiment of the present invention, the cosmetic composition according to the present invention is a metal ion sequestering agent, a humectant, a vegetable oil, an ester oil, a sunscreen, an antioxidant, and a pH adjusting agent commonly used in the art within the range of maintaining stability. , Colorants, flavoring agents, preservatives, thickeners, emulsifiers, solubilizers and plant extracts may be further included.

In one embodiment, the cosmetic composition is Oil-in-Water (water-in-water type) (O / W), Water-in-Oil (water-in-oil type) (W / O), Water-in-Silicone (silicone-in-water type) (W /S) or Water-less.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this is presented as a preferred example of the present invention and cannot be construed as limiting the present invention by this in any sense. Contents not described herein can be technically inferred by those skilled in the art, so descriptions thereof will be omitted.

Examples and Comparative Examples

Preparation Example: Preparation of a mixture of Swiss glacial water and succinic acid

(1) Swiss glacier water: Glacier water derived from Valais, Switzerland was prepared.

(2) Succinic Acid: An Iodobacter ssp. strain extracted from soil under a glacier in Valais, Switzerland was prepared. Then, the Iodobacter species strain was inoculated into a TSA medium containing 0.1 to 20 g/L of glucose, 15 g/L peptone, and 5/L tryptone, and fermented at 25 to 30 ° C. for 16 to 24 hours. Fermentation was prepared by performing twice. After inactivating the fermented product, an extract was prepared by solvent extraction using ethanol. The extract was heat-treated at 100 to 125° C. for 1 to 60 seconds, then further aged at 25 to 35° C. for 20 to 48 hours and then filtered to prepare a filtrate; Then, the fluidized layer of the filtrate was dried to obtain succinic acid.

(3) Mixture preparation: The succinic acid and Swiss ice water were mixed in a weight ratio of 1:500 to 1:600, and a powder formulation mixture was prepared by a known method. The above-prepared Swiss glacial water and succinic acid mixture was evaluated as follows.

Test Example 1-1: Measurement and evaluation of skin protection efficacy from heat stress

Human epidermal cells were subjected to heat stress treatment (heat treatment at 39° C. and 41° C. for 21 hours, respectively) using the mixture of the above Preparation Example. In this evaluation, the mixtures were treated at concentrations of 0.005%, 0.01% and 0.05%, respectively. As an index of heat stress, viability (%) of activated cells was measured through MTT assay, and the results are shown in FIG. 1 below.

1 is a graph comparing the cell viability increase rate (%) of human epidermal cells treated with a mixture of succinic acid and Swiss ice water according to the present invention after heat stress treatment, compared to a control group (untreated group). Referring to the results of FIG. 1, it was found that the human epidermal cells treated with the mixture increased the viability of the cells compared to the control group and had excellent skin protection effects from heat stress.

Test Example 1-2: Measurement of chaperone expression in senescent epidermal cells

Aged human fibroblasts (epidermal cells) were treated with the mixture of Preparation Example and then incubated for 24 hours, and then the endoplasmic reticulum (ER) chaperone gene expression of the senescent fibroblasts was measured, and the results are shown in Table 2 below.

Figure 2 is a graph showing the chaperone expression level (%) of aged epidermal cells treated with the mixture of succinic acid and Swiss ice water according to the present invention, based on the chaperone expression level (100%) of the control group (untreated group). Referring to the results of FIG. 2, it was found that the mixture-treated senescent epidermal cells increased the intracellular endoplasmic reticulum (ER) chaperone gene expression rate by 50 to 100% compared to the control group, and thus were excellent in maintaining the homeostasis of senescent epidermal cells.

Test Example 1-3: ATP production increase measurement evaluation

A "cellular sleep deprivation model" was used to mimic the effects of sleep deprivation on skin cells in a cell culture environment. The "sleep deprivation model" was created by combining damaged mitochondria with increased ER stress properties to mimic the characteristics of cells damaged during sleep deprivation. Human keratinocytes were treated with the mixture of the present invention at a concentration of 0.01%, and ATP production was measured after 2 hours of incubation, and a control group (untreated mixture group) and the results are shown in FIG. 3.

Figure 3 is a graph comparing the cellular ATP production rate (%) of human keratinocytes treated with the mixture according to the present invention in a sleep deprivation model. Referring to the results of FIG. 3, it was found that human keratinocytes treated with the mixture in the sleep deprivation model increased ATP generation in keratinocytes by 27.5% compared to the control group, and were excellent in restoring energy levels of cells.

Test Example 1-4: ER stress reduction measurement evaluation

A "cellular sleep deprivation model" was used to mimic the effects of sleep deprivation on skin cells in a cell culture environment. The "sleep deprivation model" was created by combining damaged mitochondria with increased ER stress properties to mimic the characteristics of cells damaged during sleep deprivation. Human epidermal cells thus prepared were treated with a mixture at a concentration of 0.01%, and after incubation for 2 hours, MAM (mitochondrial associated membrane) was measured as an ER stress index, and the results are shown in FIGS. 4 and 5 below.

Figure 4 is a graph comparing the effect of reducing ER Stress after treatment of the mixture of the present invention on human epidermal cells in a sleep deprivation model, and Figure 5 is a graph comparing the treatment of the mixture of the present invention on human epidermal cells in a sleep deprivation model It is a fluorescence micrograph comparing the ER Stress reduction effect of the endoplasmic reticulum. 4 and 5, the effect of reducing endoplasmic reticulum stress of the present invention is compared to healthy epidermal cells (100%), in the sleep deprivation model, the control (mixture untreated group) and the protein interaction of epidermal cells treated with the mixture at a concentration of 0.01% It was evaluated by comparing the amount of change (%).

Referring to FIGS. 4 and 5, it can be seen that the epidermal cells treated with the mixture of the present invention in the sleep deprivation human epidermal cell model have an excellent effect of maintaining cellular homeostasis by reducing the ER stress of the cells compared to the control group there was.

Test Example 1-5: Measurement and evaluation of complexion improvement

The complexion improvement effect of the mixture of the present invention was evaluated. Specifically, 23 Korean women between the ages of 41 and 57 who were overworked or did not have good sleep quality were measured for wrinkle depth using PRIMOS and skin radiance using Visia CR. Thereafter, a mixture of cream formulations prepared by a known method was applied to half of the facial part, and a placebo cream was applied to the other half, and it was used twice a day for 14 days, and the results are shown in FIGS. 6 to 8 below.

Figure 6 is a graph measuring the maximum wrinkle depth change when the mixture of the present invention was used for 2 weeks in a clinical group with poor sleep quality, and Figure 7 is a graph measuring the change in maximum wrinkle depth when the mixture of the present invention was used in a clinical group with poor sleep quality for 2 weeks 8 is a graph showing the effect of improving skin radiance when the mixture according to the present invention was used for 2 weeks in a clinical group with poor sleep quality. 6 to 8, the initial condition means the wrinkle depth and skin radiance measurement values of the clinical group before using the placebo and the mixture.

Referring to the results of FIGS. 6 to 8, when the mixture was applied to a clinical group with poor sleep quality, the depth of wrinkles was reduced by 11.4% and skin radiance was increased by 9.2% compared to the placebo formulation based on the initial condition. Through this, it was found that the effect of improving skin wrinkles and complexion was excellent.

Example 1

A cosmetic composition of an essence formulation was prepared in a conventional manner by applying the ingredients and contents of the conditions in Table 1 below. In Table 1, the mixture of Swiss glacial water and succinic acid of Preparation Example (succinic acid: glacial water = 1:500 to 1:600 weight ratio) was used.

Examples 2-5

A cosmetic composition was prepared in the same manner as in Example 1, except that the components and contents of Table 1 were applied.

Example 6

Using a phospholipid compound (lecithin compound) and maltodextrin in the contents shown in Table 1 below, a cosmetic composition of an essence formulation containing liposomes was prepared by a known method. The liposome is supported in a phospholipid layer containing a lecithin compound and maltodextrin and a carrier containing a mixture of glacial water and succinic acid (support: phospholipid layer = 1:15.7 weight ratio) supported inside the phospholipid layer. ) were included.

Comparative Examples 1 to 4

A cosmetic composition was prepared in the same manner as in Example 1, except that the components and contents of Table 1 were applied.

Test Example 2-1: Evaluation of skin irritation

A skin patch test was conducted to investigate skin irritation of the cosmetic compositions of Examples 1 to 6 and Comparative Examples 1 to 4. The subjects were selected as adult males and females between the ages of 19 and 59 without skin disease at the test site, and the average age was 47.8 years. It was conducted for a total of 10 groups of 10 people each. After wiping the patch area, such as the back, with 70% ethanol and drying, 25 μl of each of the cosmetic compositions of the above examples and comparative examples was added dropwise, and the adhesive type (pack, patch, etc.) test substance was 1cm x 1cm (width x length) Cut and attach to the skin. After the patch was applied for 24 hours, the patch was removed and the skin reaction was examined 1 hour later, and the next day (48 hours after the start of the patch) was examined again. Skin reaction determination was determined according to the following test criteria. The skin patch test results are shown in Table 3 below.

* Skin reaction test criteria

- No response ± Weak positive reaction (erythema) + Positive reaction (erythema) ++ Strong positive reaction (erythema, edema) +++ Severe positive reaction (erythema, edema, blisters)

Referring to the results of Table 3, it was found that the cosmetic compositions of Examples 1 to 5 had little skin irritation.

Test Example 2-2: Formulation stability evaluation

Stability of the cosmetic compositions of Examples 1 to 6 as representative of the above Examples and Comparative Examples was measured for the degree of separation and discoloration. Specifically, the example composition was stored in a sample container under conditions of a thermostat constantly maintained at 45 ° C and 25 ° C, a refrigerator maintained constantly at 4 ° C, and daylight conditions, and the degree of separation and discoloration was compared and measured after storage for 4 weeks. are shown in Table 4 below. At this time, the degree of separation and discoloration of the product was classified into the following 6 grades and evaluated.

* Criteria for separation and discoloration evaluation

0: No change 1: Very little separation (discoloration) 2: Slight separation (discoloration) 3: Slightly severe separation (discoloration) 4: Severe separation (discoloration) 5: Extreme separation (discoloration)

Referring to the results of Table 4, it was found that Examples 1 to 6 had excellent formulation stability.

Test Example 2-3: Measurement and evaluation of fine wrinkles (glabella, eye rims, nasolabial folds)

Among the cosmetic compositions of Examples and Comparative Examples, Example 1 was representatively evaluated for elasticity. As the test site, fine wrinkles (glabellar, eye rims, and nasolabial folds) were photographed and analyzed using Antera 3D (Miravex, Ireland). After imaging the skin surface, Antera 3D applies mechanical analysis values using an analysis program provided by the device. The measured image is analyzed for fine wrinkles using the Fine line mode of the Antera 3D program, and the measurement variable is Maximum depth. As the measured value decreases, it means that fine wrinkles are improved, and the unit is mm.

It was photographed using Antera 3D (Miravex, Ireland), and instrumental analysis values were applied using the analysis program provided by the device. The evaluation was performed by comparing the maximum depth (mm) within the group before product use (0 weeks), immediately after product use, and after 2 weeks of product use, and the results are shown in Table 5 and FIGS. 9 to 12 below.

9 is a graph comparing the effect of improving fine wrinkles (glabella) when Example 1 is applied, FIG. 10 is a graph comparing the effect of improving fine wrinkles (around the eyes) when Example 1 is applied, and FIG. It is a graph comparing the effect of improving fine wrinkles (smile line), and FIG. 12 is a photograph showing the effect of improving fine wrinkles (glabella, eye rims, and nasolabial folds) when Example 1 was applied.

Referring to Table 5 and FIGS. 9 to 12, Example 1 shows the measurement results of fine wrinkles before and after using the test product, 0.0370 mm before product use (0 weeks), 0.0354 mm immediately after product use, and 0.0341 mm after 2 weeks of product use. Statistically significant decrease (p<0.05). In addition, the improvement rate was 3.95% right after using the product and 6.37% after 2 weeks of using the product compared to before using the product (week 0). In addition, in Example 1, as a result of measuring fine wrinkles around the eyes before and after using the test product, it was statistically significantly reduced to 0.0296 mm before product use (0 weeks), 0.0278 mm immediately after product use, and 0.0266 mm after 2 weeks of product use (p < 0.05). In addition, compared to before using the product (week 0), the improvement rate was 5.09% immediately after using the product and 8.78% after 2 weeks of using the product. In addition, as a result of measuring fine wrinkles before and after the use of the test product in Example 1, there was a statistically significant decrease (p<0.05 ). In addition, compared to before using the product (0 weeks), the improvement rate was 7.43% right after using the product and 9.62% after 2 weeks of using the product, so it was found that the effect of reducing fine wrinkles between the glabellars, around the eyes and nasolabial folds was excellent.

Test Example 2-4: Rapid elasticity measurement evaluation

Among the cosmetic compositions of Examples and Comparative Examples, examples 1, 6, and Comparative Example 2 were typically measured and evaluated for elasticity. The test site was the face, and the instantaneous elasticity of the test site was measured using a Cutometer MPA 580 (Courage + Khazaka electronic GmbH). The cutometer is based on a suction method in which the skin is mechanically deformed with negative pressure, and during measurement, the negative pressure (determinism) and the skin resistance that can return to the original position (elasticity) are displayed as a curve (mm/hour unit) in real time. The larger the aperture of the probe, the deeper the skin elasticity can be measured. An 8mm probe was used to measure elasticity in the skin, and the measurement variable was R2. As the measured value increases, it means that elasticity improves, and the unit is percent (%).

The test site was measured three times using a Cutometer MPA 580 (Courage + Khazaka electronic GmbH) 8mm probe, and the average value was treated as a measured value. Evaluation was carried out by comparing the R2 (%) of Examples and Comparative Examples before product use (0 weeks), immediately after product use, and after 2 weeks of product use, and the results are shown in Table 6 and FIG. 13 below.

(measurement value unit: %)

13 is a graph comparing the effect of improving elasticity in the skin when Example 1 is applied. Referring to the results of Table 6 and FIG. 13, Examples 1 and 6 showed results of measuring skin elasticity before and after using the test product, 67.715%/66.814% before product use (0 weeks), and 71.297%/72.021% immediately after product use. , 73.088%/73.062% after 2 weeks of product use, a statistically significant increase (p<0.05). In addition, compared to before product use (0 weeks), improvement rates of 5.35% and 7.81% immediately after product use and 8.17% and 9.41% after 2 weeks of product use, respectively, Examples 1 and 6 are more elastic than Comparative Example 2. It was found that the improvement effect was excellent.

Test Example 2-5: Luminance (gloss) measurement evaluation

Among the cosmetic compositions of the Examples and Comparative Examples, the skin radiance (gloss) of Example 1 was representatively measured and evaluated. The test site was the face, and skin radiance (gloss) was measured using a Skin-Glossymeter GL200WL. GL200WL evaluates the radiance (shine) of the skin surface by light reflectance. When the LED light from the probe illuminates the skin, a portion of the light penetrates the skin surface and a portion is scattered. At this time, the diffuse reflection part is removed from the part of the directly reflected light, and the gloss value not affected by the skin color is measured. It is a measurement variable DSC (Diffuse Scattering Correction, G.U), and it means that as the measurement value increases, the skin radiance (gloss) improves.

The test site was measured three times using the Skin-Glossymeter GL200WL, and the average value was treated as the measured value. In the evaluation, DSC (G.U.) before (0 weeks), immediately after product use, and after 2 weeks of product use were compared within the group of Examples and Comparative Examples, and the results are shown in Table 7 and FIG. 14 below.

(measurement unit: TDC)

14 is a graph comparing the effect of improving skin radiance (gloss) when Example 1 is applied. Referring to the results of Table 7 and FIG. 14, Examples 1 and 6 measured skin radiance (gloss) before and after using the test product, 6.516 G.U/6.445 G.U before product use (0 weeks), and 7.178 G.U/ right after product use. 7.206 G.U., and 7.691 G.U/7.805 G.U after 2 weeks of product use, a statistically significant increase (p<0.05). In addition, compared to before product use (0 weeks), improvement rates of 10.81% / 11.81% immediately after product use and 18.96% / 21.10% after 2 weeks of product use, Examples 1 and 6 improved skin radiance (gloss) It was found that the effect was superior to that of Comparative Example 2.

Test Example 2-6: Skin Moisture Measurement Evaluation

Among the cosmetic compositions of Examples and Comparative Examples, representative examples 1, 6 and Comparative Example 3 were measured and evaluated for moisture on the skin surface. Specifically, the skin moisture content of the facial area was measured using a corneometer before using the products of Examples and Comparative Examples, immediately after using the products, and after 2 weeks of using the products. The measurement of the instrument consists of the capacitance of the current through the probe. Since the ?t amount of moisture and the electrostatic load capacity are proportional to each other, it means that moisture lasts as the measured value increases. The unit of measurement is A.U. (arbitrary uint).

The moisture content of the test site was measured using a corneometer, and the changes in Examples and Comparative Examples before product use (0 weeks), immediately after product use, and after 2 weeks of product use were compared and evaluated within the group. The improvement rate (%) was calculated as follows, and the results are shown in Table 8 and FIG. 15 below.

(measurement unit: A.U)

* Improvement rate (%) = [(measured value after product use n - measured value before product use (0 weeks)) / measured value before product use (0 weeks)] x100 (*n: right after, 2 weeks)

15 is a graph comparing the skin surface moisturizing improvement effect when Example 1 is applied. Referring to the results of Table 8 and FIG. 15, Examples 1 and 6 measured the skin moisture content before and after using the test product, 47.167 A.U./48.019 A.U. before product use (0 weeks), and 77.511 A.U./80.195 A.U. , 56.547A.U. after 2 weeks of product use. /58.634 A.U., a statistically significant increase (p<0.05). In addition, compared to before product use (0 weeks), 67.21% / 70.03% immediately after product use and 20.14% / 22.11% after 2 weeks of product use showed an improvement rate (%), so Examples 1 and 6 are in Comparative Example 2 It was found to be effective in improving skin moisture content.

Test Example 2-7: Moisture measurement evaluation in the skin (2.5 mm)

Among the cosmetic compositions of Examples and Comparative Examples, examples 1, 6, and Comparative Example 3 were measured and evaluated for moisturizing in the skin (2.5 mm depth). Specifically, using the M25 probe of Moisturemeter D, the moisture (deep moisturizing) of the facial skin at a depth of 2.5 mm was measured before (0 weeks), immediately after using the product, and 2 weeks after using the product. It consists of an electrical control device and a probe that measures tissue dielectric constant. It generates 265MHz high frequency and sends it to the skin with the probe on the same axis, and measures the moisture content of the skin by the reflected wavelength. As the measured value increases, it means that moisture (inner moisture) is improved, and the measurement unit is TDC (The tissue dielectric constant Uint).

Moisturemeter D was used to measure the amount of moisture in the skin of the test site at 2.5 mm, and the changes between Examples and Comparative Examples before product use (0 weeks), immediately after product use, and after 2 weeks of product use were compared and evaluated within the group. The improvement rate (%) was calculated as in Test Example 2-6, and the results are shown in Table 9 and FIG. 16 below.

(Unit of measure: TDC)

16 is a graph comparing the moisturizing improvement effect in the skin (2.5 mm) when Example 1 is applied. Referring to the results of Table 9 and FIG. 16, Examples 1 and 6 measured the amount of moisture in the skin (2.5mm) before and after using the test product, 33.00TDC / 32.58TDC before product use (0 weeks), 36.21 immediately after product use TDC/36.98TDC and 36.59TDC/38.07TDC after 2 weeks of product use were statistically significantly increased ( P <0.05). In addition, compared to before product use (0 weeks), 10.13% / 13.51% immediately after product use and 11.38% / 15.31% after 2 weeks of product use showed an improvement rate (%), so Examples 1 and 6 are in Comparative Example 2 It was found to be excellent in improving the moisture content in the skin (2.5 mm) compared to

Test Example 2-8: Skin texture (roughness) measurement

Among the cosmetic compositions of Examples and Comparative Examples, the skin texture (roughness) of Examples 1, 6 and Comparative Example 4 was measured and evaluated. Using Antera 3D, the skin texture (roughness) of the facial area was measured before using the products of Examples and Comparative Examples, immediately after using the products, and after 2 weeks of using the products. Antera 3D captured the same location of the test area in the texture mode of the 3D imaging device, and then analyzed the captured image using the provided software program. It was analyzed using the Roughness (Ra) value representing the roughness of the facial part, and it means that the roughness improved as the measured value decreased. The measured value unit is μm, and the improvement amount is calculated as in Test Example 2-6, and the results are shown in Table 10, FIGS. 17 and 18 below.

(measurement value unit: ㎛)

* Improvement rate (%) = │ (measured value after product use n - measured value before product use (0 weeks)) / measured value before product use (0 weeks) │ x100 (*n: right after, 2 weeks)

17 is a graph showing the skin texture (roughness) measurement results of Example 1, and FIG. 18 is a photograph showing the skin texture improvement effect of Example 1. Referring to Table 10 and FIGS. 17 and 18, Examples 1 and 6 show skin texture (roughness) (Ra) measurement results before and after using the test product, 5.8442 μm / 5.7261 μm before product use (0 weeks), product use Statistically significant decrease to 5.5831㎛/5.3914㎛ immediately after and 5.4419㎛/5.2257㎛ after 2 weeks of product use (p<0.05). In addition, compared to before product use (0 weeks), they showed an improvement rate (%) of 4.01% / 5.85% immediately after using the product and 6.17% / 8.74% after 2 weeks of using the product, so Examples 1 and 6 are in Comparative Example 4 It was found to be effective in improving skin texture (roughness) compared to

Experimental Example 2-9: Sensory evaluation

When the cosmetic compositions of Examples and Comparative Examples were applied to the skin, the feeling of use was evaluated. Specifically, a questionnaire about the feeling of use was conducted with subjects consisting of 50 trained women aged 20 to 50 years. After using the cosmetic compositions of Examples 1 to 6 and Comparative Examples 1 to 4 once a day, the results were evaluated in a manner in which the items of moistness, durability, spreadability and gloss were answered on a 5-point scale (higher the score, the better). are shown in Table 11 below.

Referring to the results of Table 11, it was found that Examples 1 to 6 of the present invention were superior to Comparative Examples 1 to 4 in terms of moistness, durability, spreadability and gloss.

So far, the present invention has been looked at mainly through embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (9)

a phospholipid layer; and
A liposome comprising a carrier supported on the phospholipid layer and containing Swiss glacial water and succinic acid derived from an Iodobacter ssp. strain as an active ingredient,
The liposome is a cosmetic composition comprising the carrier and the phospholipid layer in a weight ratio of 1:3 to 1:25.
The cosmetic composition according to claim 1, wherein the Iodobacter species strain is collected from soil in Valais, Switzerland.
The cosmetic composition according to claim 1, which comprises 0.0001 to 10.0% by weight of the Swiss ice water and 0.00001 to 0.05% by weight of succinic acid, based on the total weight of the cosmetic composition.
delete The cosmetic composition according to claim 1, wherein the cosmetic composition has a skin protecting effect from skin thermal damage and oxidative stress.
The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of improving skin complexion by improving skin wrinkles and roughness.
The cosmetic composition according to claim 1, wherein the cosmetic composition has an external moisturizing effect and an internal moisturizing effect.
The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax or spray formulation Phosphorus cosmetic composition.
The method of claim 1, wherein the cosmetic composition is Oil-in-Water (O/W), Water-in-Oil (Water-in-Oil) (W/O), Water-in-Silicone (Water in Silicone) (W / S) or Water-less (anhydrous) cosmetic composition.

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