KR100702327B1 - Cosmetic material including extract of camellia flower for skin abirritation - Google Patents

Abstract

The present invention relates to a cosmetic skin irritation agent containing Camellia Japonica L. extract.

Camellia extract according to the present invention has a skin soothing effect by anti-inflammatory action and anti-allergic action, very little side effects can be applied to infants as well as adults, irritation such as inflammation, allergy, edema, erythema, etc. when applied to the skin It is used as a safe cosmetic because it has excellent relief and prevention.

Camellia extract, skin irritation relief, anti-inflammatory, anti-allergy, cosmetics

Description

Cosmetic material including extract of camellia flower for skin abirritation}

The present invention relates to a cosmetic skin irritation agent containing Camellia Japonica L. extract. More specifically, it has anti-inflammatory, anti-allergic effect, and has excellent effect of preventing and alleviating skin irritation such as inflammation, allergy, edema, erythema, etc., which may occur when applied to the skin, and contains camellia flower extract with very few side effects. It is about cosmetics.

The skin surface of the human skin is sebaceous, which is naturally mixed with sebum secreted from sebaceous glands and moisture from the glands, covering the stratum corneum, so that it remains smooth, shiny and moist. When the water content of the stratum corneum is about 20%, the skin becomes the most ideal and beautiful state.When the water content of the stratum corneum falls below 10%, the keratin is released from the skin and foreign matters are easily penetrated. And allergy is easy to induce. In particular, as the age of the skin metabolism is not smooth, various inflammation and allergies are aggravated.

In addition, people are becoming more sensitive to skin by external stimuli such as dry indoor environment, pollution, ultraviolet rays, wind, misused cosmetics and detergents. The skin, which is sensitive by external stimuli, may be simply visible, such as erythema, and may have severe and unpleasant inflammatory reactions, such as itching and itching, so it may be a little burdensome and persistent to use popular skin cosmetic compositions. To refrain.

Cosmetics are products used to protect the skin and cleanse the beauty, but when looking at the cosmetic composition in detail, components different from the original skin protection purpose is used as essential to the product formation. For example, solubilizers, emulsifiers, preservatives, fragrances, sunscreens and other additives used to impart efficacy and effects, generally these ingredients may cause inflammation, allergies, rashes or edema to the skin. It is known to cause the main cause of the substance.

Inflammation is a series of defenses aimed at minimizing the response and restoring damaged areas when cells or tissues are damaged by any cause. Inflammation is the cause of inflammation, including physical factors such as trauma, burns, frostbite, and radiation. There are chemical factors caused by chemicals such as acids and bases, and immunological factors caused by antibody reactions. They can also be caused by vascular or hormonal imbalances.

Histamine is the most well known substance that mediates itching and pain. So far it has been found that there is no specific receptor for detecting itching, and it is presumed to be due to an unspecified autonomic nerve ending in the gap between epithelial cells or the dermis and epidermis, such as local pain. The substances that mediate itching and pain are mainly released from mast cells and released by cross-linking of the Fcε RIα receptor. In addition, histamine may be released from skin mast cells by inflammatory mediators such as substrate P, hepatocyte factor, vasoactive intestinal peptide and the like apart from the Fcε RIα receptor.

To this end, chemicals that can be used to alleviate irritation and inflammation such as erythema and edema are nonsteroidal substances such as flufenamic acid, ibuprofen, benzydamine, and indomethacin. Indomethacin, and steroidal substances include prednisolone and dexamethasone. Lonic acid, hydrocortisone, licorice acid and its derivatives (β-glycileic acid, glycylinic acid derivatives) are also known to have anti-inflammatory effects (Gwang Jeong-dong, Shin Cosmetics, Namsan Sugar, p162, 1993).

However, the cause of skin side effects in cosmetics is always latent, and various studies have been conducted to solve this problem. As a result, indomethacin, which is used as an anti-inflammatory agent, cannot be used in cosmetics, and hydrocortisone has a limited amount. Licorice acid and its derivatives are difficult to stabilize or have poor solubility, and thus, in actual production, anti-inflammatory agents are known to have a practical effect due to concentration limitation. It has a problem and its use is limited.

Recently, research and development on cosmetics using natural raw materials that can be used for the purpose of alleviating irritation and inflammation in order to meet the needs of consumers has been actively conducted.

On the other hand, camellia is a flower of Camellia Japonica L. , an evergreen sub-tree, which belongs to the tea tree family, and has a height of 7M. Camellia grows on the shores or islands of Korea's Ulleungdo, Jeju, and southern regions. The flowering period is from December to May and the fruiting season is from September to October. Dried camellia, used as a medicinal herb, has a supple and good fragrance. The reddish color is not moldy, the buds are large, and the highest grade is not yet blooming. The taste and nature of sandaflower is sweet, a bit very and cool.

The effect of camellia is liver and menopause, and it is effective for sheep blood, hemostasis, and acid fish. For clinical application and usage, it is taken on earth, blood, abolism, erythema, intestinal bleeding, etc., and burns with burned oil. In the Herbal Wood Milk Milk, it is said that the treatment of dental bleeding is made by grinding Boju Sanda into powder and pouring warm water.

The components of the camellia are leucoanthocyanin and anthocyanin (Tongyang Medical University Encyclopedia, Kyung Hee University Publishing, Oriental Medical Dictionary Dictionary Committee, p540-541). In addition, camellin B, quercetin as flavanol, kaemferol, sexangularetin and phenol as phenolic compounds, ρ-hydroxybenzoic acid, proto Protocatechuic acid, gallic acid and other sterols and 28-nor-oleanene triterpenes are contained ( Chem. Pham. Bull. 37). (11) 3174-3176 (1989), Chem. Pham. Bull. 44 (10) 1899-1907 (1996), YAKUGAKU ZASSHI, 104 (2) 157-161 (1984)).

Prior arts related to camellia have been described in Korean J. Plant. Res. 17 (3) 314-322 (2004) for the antioxidant and antimicrobial activities of Camellia Japonica L. extracts in Korea. In addition, Korean Patent Laid-Open Publication No. 2004-101709 discloses that a cosmetic composition containing a nanoliposomal stabilized Camellia extract is effective in preventing aging such as whitening, moisturizing, and elasticity, and Japanese Patent Laid-Open No. 2002-371092. Japanese Patent Laid-Open Publication No. 55-099180 discloses a method of making tea by fermentation of camellia, and Japanese Laid-Open Patent Publication No. 55-111758 No. describes a method for producing a similar chocolate from camellia flowers, but all of the prior art are irrelevant to cosmetics having a skin irritation relief effect to be achieved in the present invention.

Accordingly, the present inventors continued to study the use of the camellia extract resulted in the excellent safety on the skin and easy to stabilize the preservation when formulating cosmetics and has a substantially superior skin irritation relief effect and completed the present invention.

An object of the present invention is to provide a cosmetic skin irritation relaxing agent containing Camellia Japonica L. extract as an active ingredient. More specifically, by containing a camellia extract, the anti-inflammatory action, anti-allergic action is excellent in the prevention and relief of skin irritation with skin soothing effect, and to provide a safe cosmetic with very few side effects.

The present invention relates to a cosmetic skin irritation agent containing Camellia Japonica L. extract as an active ingredient. More specifically, it contains anti-inflammatory, anti-allergic, and camellia extracts that have a soothing effect on the skin. This very small topic relates to safe cosmetics that can be applied to infants as well as adults.

Camellia extract of the present invention is prepared by the following method.

1) The dried camellia is selected by weight from the group consisting of water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, and propylene glycol as extraction solvent. Add 5-20 times the volume of one or more solvents. Extraction method is a method of extracting by heating at 50 ~ 100 ℃, 3 to 20 hours in the state of preventing the evaporation of the solvent is equipped with a cooling condenser, or by extracting the active ingredient by deposition for 1 to 15 days at 5 ~ 37 ℃ You can also use both extraction methods. The Camellia extract thus extracted is concentrated under reduced pressure while recovering the solvent evaporated from the distillation apparatus equipped with a cooling capacitor, and then lyophilized or spray dried to obtain a dry extract.

2) The obtained extract was suspended in distilled water, and then fractionated sequentially using hexane, methylene chloride and n-butanol. The solvent of the n-butanol fraction was concentrated under reduced pressure, and then lyophilized or spray dried to obtain a dry extract.

Camellia extract can be used as appropriate in a wide range of 0.01 to 10.0% by weight in terms of solid content, preferably 3.0 to 10.0% by weight.

The cosmetics of the present invention may contain various conventionally known additives and include coloring agents, flavoring agents, suspending agents, emulsifiers, dissolving aids, stabilizers, isotonic agents, pH adjusting agents, viscosity adjusting agents, solvents, and the like. .

In addition, the cosmetic of the present invention may be prepared into various kinds of cosmetic compositions by containing an appropriate additive depending on the use and method of use. Formulations that can be used include lotion (skin lotion), nourishing lotion, nourishing cream, massage cream, nourishing essence, emulsified foundation, etc., for infants, lotion (skin lotion), lotion, cream, It can be manufactured and used as a sunscreen lotion, a sunscreen cream, etc., and can also be manufactured and used for bath cleaners, shampoos, soaps etc. for infants.

The following examples illustrate the content of the invention, but the content of the invention is not limited thereto.

Reference Experimental Example 1. Anti-inflammatory test in dermis for each part of camellia (measured IL-1α release)

In order to examine the anti-inflammatory effect on each part of the camellia, each part was extracted with 95% ethanol, filtered, concentrated under reduced pressure, and used as a sample. Extraction conditions of the extract for each site are the same. After culturing the dermis, the irritation was induced, and the release amount of IL-1α of each extract was examined.

IL-1α (interleukin-1α) is an inter-inflammatory cytokine that acts as a pre-inflammatory mediator. Osteoblasts, monocytes, macrophages, keratinocytes are stimulated by inflammation and infection. Cells, hepatocytes, fibroblasts, etc. temporarily increase production. IL-1α also acts as a potential derivative of wound re-epithelialization, as well as leukotriene B ₄ , 5-, 12-, 15 HETE, which promotes leukocyte movement as a regulator of allergic and inflammatory responses. It is known to enhance the production of arachidonic acid lipoxygenase metabolites.

A buffer solution prepared with Type I collagen matrix (Bioland, Korea) and 5-fold concentrated DMEM (Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), 2.2% sodium bicarbonate, 200 mM HEPES, 0.05 N sodium hydroxide, Human fibroblast cell line (ATCC, Cat.No. CRL-2076) made of 1 was mixed at a density of 1 × 10 ⁵ cells / ml, and the collagen solution containing 200 μl of fibroblasts was mixed in a 10 mm diameter insert. Prepare the dermis. After dermal preparation, the DMEM containing 10% FBS is replaced every 2 days and incubated for 7 days. 10 μl of skin stimulant SLS (sodium lauryl sulfate) was administered to the cultured dermis, and after 4 hours, 10 μl of each extract was incubated at 37 ° C. and 5% CO ₂ for 24 hours. The solvent of SLS and extract was DMSO and the concentration was the weight percentage of the toxic substance in the solution. IL-1α was sampled using a human IL-1α assay kit (Endogen Inc. Boston, MA, USA) and measured at 450 nm by ELISA-reader (enzyme-linked immunosorbent assay). The results are shown in Table 1.

TABLE 1 Anti-inflammatory Effects in the Dermis

sample Final concentration of the extract contained in the reaction solution (㎍ / ml) IL-1α release amount (pg / ml) SLS 0.07% 82 Camellia leaves 100 73 Camellia Branch 100 79 Camellia bark 100 71 Camellia root 100 69 Camellia Flower 100 65 Camellia Seeds 100 75

As shown in Table 1, IL-1α emission was decreased the most in camellia flower (hereinafter called camellia flower), which showed the best anti-inflammatory effect.

Example 1.

500 g of completely dried camellia flower was placed in 10 kg of 95% ethanol, extracted by boiling in an extractor equipped with a cooling condenser for 5 hours, filtered through a 400 mesh filter cloth, and cooled to room temperature. After cooling, the mixture was left to stand at 15 ° C. for 7 days to ripen, and then filtered through Whatman No. 2 filter paper. The extract was filtered again with a 0.45 μm filter and concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 20.2 g (dry weight) of camellia extract.

Example 2.

The vacuum concentrate of Example 1 was suspended in distilled water to remove the hexane fraction using 1 kg of hexane, and then the aqueous layer was suspended with 1 kg of methylene chloride to remove the methylene chloride layer to obtain an aqueous layer. 1 kg of n-butanol was suspended in the obtained water layer to remove the water layer, and an n-butanol layer was taken and concentrated under reduced pressure to obtain 8.6 g (dry weight) of a camellia extract.

Examples 3-9

Using the solvent of Table 2 below and extracted in the same manner as in Example 1 and the results are shown in Table 2 below.

<Table 2> Experimental conditions of Examples 3-9

menstruum Dry weight [g] Example 3 Purified water 13.2 Example 4 30% ethanol 21.6 Example 5 30% glycerin 19.3 Example 6 30% Butylene Glycol 18.7 Example 7 80% methanol 19.8 Example 8 80% acetone 20.4 Example 9 Ethyl acetate 13.7

In order to measure the skin protection effect from the damage caused by allergy and inflammation for the camellia extract was tested as follows.

Experiment 1. Assessment of allergen (LLNA assay)

A local lymph node assay (LLNA) was performed to confirm allergy-induced camellia extract. 25 μl of a sample (camellia extract) was applied to the mouse ears once a day for three days using a mouse that had undergone a one-week period of purifying. After 4 days, the lymph nodes were removed and the lymphocytes were separated. After incubation, the degree of amplification was measured by the insertion amount of radioisotope [ ³ H] -methylthymidine, and the results are shown in Table 3. The local lymph node evaluation (LLNA) results indicate the lymphocyte amplification of the sample group relative to the control group and are considered to be antigens when the stimulation index (SI) of the sample is 3 or more.

<Table 3> Evaluation Results of Allergy-induced Camellia Extract

sample Final concentration of camellia extract (㎍ / ml) Average cpm Stimulus Index (S.I) Ethanol (60%) 100 2261 ± 121 - Example 1 100 3215 ± 125 1.42 Example 2 100 2993 ± 231 1.32 Example 3 100 4238 ± 241 1.87 Example 4 100 4548 ± 331 2.01 Example 5 100 4522 ± 135 2.00 Example 6 100 4002 ± 195 1.77 Example 7 100 3566 ± 301 1.58 Example 8 100 4577 ± 302 2.02 Example 9 100 4321 ± 125 1.91

Cpm (counter per minute): The amount of [ ³ H] -methylthymidine inserted into the cell

※ S.I: Average cpm of sample divided by average cpm of control

As shown in Table 3, the stimulation index (S.I) of all camellia flower extracts was 3 or less, indicating that there is little possibility of causing allergy.

Experiment 2. Antiallergic experiment (β-hexosaminidase release measurement)

β-hexosaminidase is an enzyme contained in mast cells and released together with histamine when mast cells are activated by an immune response. Therefore, β-hexosaminidase can be used as an indicator of degranulation of mast cells. (Planta Med. 64, 577-578 (1998))

Incubate the RBL-2H3 cells with EMEM (Eagle's Minimum Essential Medium) medium containing 10% FBS and glutamine. Dispense ⁵ x 10 ⁵ cells per well into a ²⁴ well plate and use the medium containing 0.5 µg / ml of mouse monoclonal immunoglobulin E in mouse EMEM medium. Incubate in a 5% CO ₂ incubator. Cells are rinsed using 500 μl of siraganian buffer I (pH 7.2, 119 mM sodium chloride, 5 mM potassium chloride, 0.4 mM magnesium chloride, 25 mM PIPES and 40 mM sodium hydroxide) per well, and Shiraga 160 μl of Nian buffer II (added with 5.6 mM glucose, 1 mM potassium chloride and 0.1% BSA) is added and incubated for 10 minutes in a 37 ° C., 5% CO ₂ incubator. After treating the camellia extract, it is incubated for 20 minutes in a 37%, 5% CO ₂ incubator and treated with the antigen (1 μg / ml, DNP-BSA) for 10 minutes. The reaction is stopped and centrifuged by an ice bath for 10 minutes. The supernatant was treated with 20 μl of substrate (1 mM ρ-nitrophenyl-N-acetyl-β-D-glucosaminid), then incubated for 1 hour in a 5% CO ₂ incubator at 37 ° C. and 0.1M Na ₂ CO ₃ / NaHCO _{Add 3} to stop the reaction. The absorbance was measured at 405 nm using an ELISA reader, and the inhibition rate was calculated as follows. The results are shown in Table 4.

※ Inhibition Rate (%) = {1- (B-C-D) / (A-C-D)} × 100

A (control): without adding camellia extract (antigen IgE reaction measurement)

B (Treatment group): Added camellia extract (antigen IgE reaction measurement)

C (blank): Only camellia extract and substrate added to ELISA plate

D (untreated group): without adding antigen, IgE and camellia extract

<Table 4> Antiallergic Evaluation Results of Camellia Flower Extract

sample Final concentration of camellia flower extract in the reaction solution (㎍ / ml)  % Inhibition Example 1 200 21.3 Example 2 200 45.7 Example 3 200 27.1 Example 4 200 34.8 Example 5 200 20.1 Example 6 200 15.3 Example 7 200 37.2 Example 8 200 39.5 Example 9 200 28,3

As shown in Table 4, all camellia flower extracts have been shown to have an antiallergic effect by inhibiting the release of β-hexosaminidase, and in particular, Example 2 shows a very high inhibition rate as 45.7%.

Experiment 3. Anti-inflammatory test for mouse ear edema

Mouse ear edema was performed to investigate the anti-inflammatory effect of Camellia extract.

Three mice per sample were used for mice that had undergone one week of acclimation. The left ear of the mouse was the control site and the right ear was the test site, and the thickness of both ears was measured first. Ears were washed thoroughly with ethanol prior to application of the sample and 20 μl / ear of sample was continuously applied once daily for 4 days. One hour after the last application, ethanol was applied to the left ear and arachidonic acid to 2 mg / ear on the right ear. Measured.

Anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group and the results are shown in Table 5.

TABLE 5 Ear thickness and inflammation inhibition rate by topical application

sample Final concentration in the reaction solution (㎍ / ml) menstruum Ear thickness average (㎛) % Inhibition Camellia Extract Before sample processing After sample processing Indomethacin 1.0 ethanol 284 421 45.6 Arachidonic acid 2mg / ear ethanol 299 551 - Example 1 10 ethanol 279 477 21.4 Example 2 10 ethanol 283 472 25.0 Example 3 10 ethanol 279 495 14.3 Example 4 10 ethanol 288 499 16.4 Example 5 10 ethanol 284 505 12.4 Example 6 10 ethanol 294 511 13.8 Example 7 10 ethanol 295 498 19.6 Example 8 10 ethanol 293 506 15.3 Example 9 10 ethanol 295 503 17.4

※ Inhibition Rate (%) = (A―B) / A × 100

A: Average thickness of control ears (thickness of arachidonic acid treated ears-thickness of untreated ears)

B: thickness of sample-coated group ears (thickness of ethanol-treated ears-thickness of untreated ears)

According to Table 5, although it is lower than indomethacin, 12.4-25% of the anti-inflammatory effect is suppressed in all samples, and thus the camellia extract has a prophylactic and therapeutic effect on anti-inflammatory, and in particular, in Example 2, It can be seen that there is a high inhibitory effect.

Experiment 4. Anti-inflammatory effect on 3D bioartificial skin (measured IL-1α release)

IL-1α (interleukin-1α) is an inter-inflammatory cytokine that acts as a pre-inflammatory mediator, and is a osteoblast, monocyte, macrophage, or keratinocyte caused by stimulation by inflammation or infection. It is a substance produced by temporarily increasing formation cells, hepatocytes, fibroblasts and the like. In addition, IL-1α is a member having a wound-leukotriene _{B 4 (leukotriene B 4),} 5-, 12- , as well as to act as potential derivatives of epithelialization (re-epithelialization) promote leukocyte movement as allergic reactions and inflammatory response regulator And 15 are known to enhance the production of arachidonic acid lipoxygenase metabolites such as HETE. After stimulating the three-dimensional artificial skin, the changes of IL-1α release by camellia flower extracts were examined.

A buffer solution prepared with Type I collagen matrix (Bioland, Korea) and 5-fold concentrated DMEM (Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), 2.2% sodium bicarbonate, 200 mM HEPES, 0.05 N sodium hydroxide, Human fibroblast cell line (ATCC, Cat.No. CRL-2076) mixed with 1 was mixed at a density of 1 × 10 ⁵ cells / ml, and collagen with 200 μl of fibroblasts mixed in a 10 mm diameter insert. Prepare the dermis by adding the solution. After the dermal preparation, the DMEM medium containing 10% FBS was replaced every 2 days and cultured for 7 days. Normal keratinocytes are inoculated onto the cultured dermis with 3 × 10 ⁵ cells / insert. 7 days in a medium containing DMEM (10% FBS) and K-SFM (Keratinocyte Serum Free Medium, GIBCO, USA) (provided with EGF (epidermal growth factor) and BPE (bovine pituitary extract)) in a 1: 1 mixture After the submerge culture of the medium, the medium in the insert was removed for air-liquid interface culture, and the gas-liquid interface culture was carried out for 14 days using the medium used for subculture only outside the insert. Was done. Remove all culture media from the cultured artificial skin, and then add 1.5 ml of fresh medium outside the tissue culture vessel. After inserting 10 μl of sodium lauryl sulfate (SLS: Sodium Lauryl Sulfate) as a skin irritant in the insert and applying 10 μl of camellia extract 4 hours later, the incubator is incubated at 37 ° C. and 5% CO ₂ for 24 hours. The solvent of SLS and Camellia extract was DMSO and the concentration was the weight percentage of the substance to the solution. Media samples were taken for measurement of IL-1α release, and human IL-1α assay kit (Endogen Inc. Boston, MA, USA) was used and measured at 450 nm by ELISA-reader (enzyme-linked immunosorbent assay). 6 is shown.

Table 6 Anti-inflammatory Effects on Three-Dimensional Artificial Skin

sample Final concentration of camellia extract contained in the reaction solution (㎍ / ml) IL-1α release amount (pg / ml) SLS 0.05% 67 Example 1 50 61 Example 2 50 53 Example 3 50 66 Example 4 50 62 Example 5 50 58 Example 6 50 55 Example 7 50 56 Example 8 50 59 Example 9 50 60

According to Table 6, in Example 2, the release amount of IL-1α was found to be reduced by more than 20%, indicating that the release amount of IL-1α was decreased in all examples, indicating that the camellia extract inhibited inflammation. Can be.

Experiment 5. Cytotoxicity Test

Cytotoxicity in monolayer cell cultures was measured for the Camellia flower extract (MTT assay, J. Immunological Methods 65 , 55 (1983)).

The MTT test is a method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are water-soluble and yellow salts of MTT (3- [4,5-dimethylthiazole-2-yl] -2,5 in the mitochondria. It is based on the principle that -diphenyltetrazolium bromide is reduced to water-insoluble blue formazan derivative by succinate dehydrogenase. It reflects the concentration of living and vigorous cells.

For experiments, human fibroblast cell lines (ATCC, CRL-2076) were inoculated in 24well plates at a concentration of 1 × 10 ⁵ cells / ml. Medium was used DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 10% bovine serum. After 24 hours of inoculation, the cells were replaced with DMEM containing 2% of bovine serum, and 10 µl of Camellia flower extract was added, followed by incubation in 37 ° C. and 5% CO ₂ incubator for 3 days. After incubation, the supernatant was removed, and MTT solution was added 1.0 ml per well. After 4 hours, MTT was removed, DMSO was added 1.0 ml per well, incubated at 37 ° C. overnight, and absorbance was measured at 570 nm.

At this time, the dissolution solvent of the camellia flower extract was used as a negative control and sodium lauryl sulfate as an anionic surfactant as a skin irritant as a positive control. The results for cell viability for each material are shown in Table 7.

Table 7 Measurement of Cytotoxicity in Monolayer Cell Culture of Camellia Extract

sample Final concentration of camellia extract contained in the reaction solution (㎍ / ml) Cell survival rate (%) SLS 0.0025% 50 Example 1 100 98 Example 2 100 103 Example 3 100 99 Example 4 100 101 Example 5 100 101 Example 6 100 96 Example 7 100 102 Example 8 100 64 Example 9 100 85

As a result, in Example 2, the cell viability of 100% or more was shown, and the cell viability was improved in all the examples, indicating that the camellia flower extract was a very safe cytotoxic substance.

As described above, camellia flower extract was found to have a safe and protective effect from skin damaged by allergy and inflammation without causing allergy. Therefore, the prescription containing camellia extract was performed as follows and the effect experiment by the prescription was done.

Prescription Example 1.

Prescription example of a lotion (skin lotion) in the cosmetic containing the camellia extract of Example 2 is as follows.

number Raw material Content (% by weight) One  Camellia Flower Extract (Example 2) 3.0 2  glycerin 3.0 3  Butylene Glycol 2.0 4  Propylene glycol 2.0 5  Polyoxyethylene Cured Castor Oil 1.0 6  ethanol 10.0 7  Triethanolamine 0.1 8  antiseptic a very small amount 9  Pigment a very small amount 10  Spices a very small amount 11  Purified water Remaining amount Sum 100.0

<Production Method of Prescription Example 1>

2, 3, 4, and 8 are sequentially added to 11 of Formulation Example 1, followed by stirring to dissolve. After dissolving 5 times by heating about 60 ℃, add 10 times and add it to 11 times. Finally, 1, 6, 7, and 9 are added to No. 11, sufficiently stirred, and aged.

Prescription Example 2.

Prescription example of nutrition lotion in cosmetics containing the camellia extract of Example 2 is as follows.

number Raw material Content (% by weight) One Camellia Flower Extract (Example 2) 3.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solbitan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Remaining amount Sum 100.0

<Method of Preparation Example 2>

10, 11, 13, and 16 of Formulation Example 2 were mixed and stirred and heated to 80-85 ° C., and then put into the manufacturing unit, and then the emulsifier was activated and 2, 3, 4, 5, 6, 7, 8, 9 And No. 12 is also heated to 80 ~ 85 ℃ and then emulsified. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add 15 times, cool down to 45 ℃, add 14 times, and when it reaches 35 ℃, add 1 and continue to cool down to 25 ℃ and mature. .

Prescription Example 3

Prescription example of nutrition cream in the cosmetic containing the camellia extract of Example 2 is as follows.

number Raw material Prescription Example 3 Prescription Example 3-1 Comparative Prescription 1 One Camellia Flower Extract (Example 2) 5.0 10.0 - 2 Stearic acid 2.0 2.0 2.0 3 Cetyl alcohol 2.0 2.0 2.0 4 Glyceryl Monostearate 2.0 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 1.0 1.0 8 Wax 1.0 1.0 1.0 9 Liquid paraffin 4.0 4.0 4.0 10 Squalane 4.0 4.0 4.0 11 Caprylic / Capric Triglycerides 4.0 4.0 4.0 12 Carboxy Vinyl Polymer 0.3 0.3 0.3 13 Butylene glycol 5.0 5.0 5.0 14 glycerin 3.0 3.0 3.0 15 Triethanolamine 0.5 0.5 0.5 16 Purified water Remaining amount Remaining amount Remaining amount 17 water - - 10.0 Sum 100.0 100.0 100.0

<Preparation Example 3, 3-1 and Comparative Method 1 Preparation Method>

1. Prescription Examples 3 and 3-1 : 12, 13, 14 and 16 while stirring and heating to 80 ~ 85 ℃ while mixing and stirring the emulsifier, and further 2, 3, 4, 5 , 6, 7, 8, 9, 10 and 11 is heated to 80 ~ 85 ℃ and then added 15 times to emulsify. After emulsification, the mixture is cooled to 35 ° C while stirring using a stirrer and 1 time is added to it, cooled to 25 ° C, and aged.

2. Comparative Example 1 : After mixing 12, 13, 14 and 16, and heated to 80 ~ 85 ℃ while stirring the emulsifier after the addition to the manufacturing unit, and 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 is heated to 80 ~ 85 ℃ and then added 15 times to emulsify. After emulsification, the mixture is cooled to 35 ° C while stirring using a stirrer, 17 times, cooled to 25 ° C, and aged.

Prescription Example 4.

Prescription example of the essence of the cosmetic composition containing the camellia extract of Example 2 is as follows.

number        Raw material Content (% by weight) One  Camellia Flower Extract (Example 2) 5.0 2 Cytostolol 1.7 3  Polyglyceryl 2-oleate 1.5 4  Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11  Xanthan Gum 0.2 12  antiseptic a very small amount 13  Spices a very small amount 14  Purified water Remaining amount Sum 100.0

<Production Method of Prescription Example 4>

2, 3, 4, 5 and 6 of Formulation Example 4 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipids were mixed with Nos. 1, 7, 8, and 14, and homogenized at a constant temperature, passed through a microfluidizer, and then, No. 9 was gradually added at a constant temperature to homogenize, and then again the microflu. Pass it back through the die. 10, 11, 12 and 13 are added and dispersed to stabilize and mature.

Experimental Example 1.Efficacy of erythema after primary skin stimulation (closed clam experiment)

Formulation Example 3, Comparative Example 3-1 and Comparative Example 1 in the nutritional cream Sodium Laurly Sulfate (sodium lauryl sulfate) to be 0.5% to confirm the skin irritation relief effect when applying the skin Clinical confirmation experiments were conducted. 20 healthy boys, girls, and children aged 3 or older (age: 3 to 8 years, average age: 5 years, 15 men) who were judged to have no current skin disease and no history of atopic dermatitis and other eczema. , 5 women).

The erythema index (E-index) is measured before application of the sample to the inner region of the lower forearm of the subject, and a large amount of each sample is measured using a large finn chamber (Epitest, Hyryla Finland) and filter paper. 0.2 g) was attached to the patch for 24 hours. The patch was removed and the erythema index measurement was performed after the subject exposed the test site in a space where there was no air movement and no direct sunlight, and then stabilized for 30 minutes.The results after 1, 2 and 1 week after removing the patch Measured. Measurements were made using a skin spectrometer (Dermaspectrometer; Cortex Technology, Hadsund, Denmark) and the results are shown in Table 8 below. The room temperature was 22 ° C and the humidity was 43%.

Table 8. Result of skin primary irritation relief test

 sample BL D0 D1 D2 W1    Comparative Prescription 1 9.7 11.8 12.6 12.3 10.5   Prescription Example 3 9.9 12.1 11.4 11.1 10.2   Prescription Example 3-1 9.5 11.6 10.2 10.1 10

BL: baseline

D0: 30 minutes after patch removal

D1: 1 day after patch removal

D2: 2 days after patch removal

W1: 1 week after patch removal

Inflammation-producing skin irritation substance that the cosmetics of Formulation Example 3, in particular, the cosmetics of Formulation Example 3-1, do more damage to the skin than the cosmetics of Comparative Formula 1, from which the camellia extract was not added It can be seen that the skin irritation relieving effect from the is excellent.

Experimental Example 2. Skin irritation test (stinging test)

Subjects showing lactic acid-sensitive irritation were first washed with soap and then applied for 15 minutes in a constant temperature and humidity room (21 ° C, 47% relative humidity).

Male, female children and children who are sensitive to lactic acid in order to confirm the skin irritation-reducing effect when applying skin stimulation to 6.0% lactic acid as a skin stimulant in the nutritional cream of Prescription Example 3 and Comparative Example 3-1 Fifteen patients (age distribution: 5 ~ 8 years old, average age: 7 years old, 12 males, 3 females) were tested as follows.

400 μl each was applied to the nonwoven fabric, and after 20 seconds after attaching the nonwoven fabric around the nose and on the cheek, which was reported to feel good tingling and itching, it was removed. After removal of the nonwoven fabric (10 seconds, 2 minutes 30 seconds, 5 minutes, 8 minutes) on the basis of the criteria in Table 9 to measure the tingling, itching degree according to the subjective judgment of the subjects are shown in Table 10 below The anti-stimulation rate was calculated and summarized in Table 11.

* Anti-stimulation rate = (control stimulus index-prescription stimulus index) x100 / control stimulus index

Table 9. Stimulation Index of Stinging and Itching

Stinging, Itching Index Judgment 0 No feeling One Slight tingling or itching 2 Tingling or itching 3 Severe tingling or itching

Table 10. Irritation index for lactic acid in each prescription

division Comparative Prescription Example 1  Prescription Example 3  Prescription Example 3-1 time 10 sec 2.5 minutes 5 minutes 8 minutes 10 sec 2.5 minutes 5 minutes 8 minutes 10 sec 2.5 minutes 5 minutes 8 minutes Hourly Stimulus index 1.2 2.0 1.4 1.0 1.0 1.3 1.1 0.5 1.0 1.1 1.0 0.2 Average index 1.4 0.98 0.83

* Stimulation index of the comparative example (lactic acid 6.0%): 1.81

Table 11. Anti-Stimulus Rates for Lactic Acid in Each Prescription

  sample Anti-irritation rate (%)    Comparative Prescription Example 1 22.6   Prescription Example 3 45.9 Prescription Example 3-1 54.1

As described above, the prescription example containing camellia extract showed that the stimulation index was lower than 1.4 of the prescription example without the stimulation index of 0.98 and 0.83, and the prescription example containing no camellia extract even in the hourly stimulation index. It can be seen that the stimulation index is always lower from 10 seconds to 8 minutes, and therefore, even in the anti-irritation rate, it can be seen that the prescription example containing camellia extract is more than twice as large as the prescription example without containing. Example 3-1 shows that the effect is greater, which can be said to be due to the camellia extract. In addition, the high anti-stimulation rate is applicable to infants as well as adults.

The present invention relates to a cosmetic skin irritation inhibitor containing Camellia Japonica L. extract as an active ingredient, the camellia extract has an anti-inflammatory, anti-allergic effect and has a soothing effect on the skin and thus inflammation upon application to the skin. It is a very useful invention that provides a cosmetic that can alleviate the skin irritation, such as allergies, edema, erythema and the like, and also has a very low side effects, so as to safely alleviate the skin irritation in the skin of infants as well as adults.

Claims (7)

A skin irritant for cosmetics containing camellia extract. According to claim 1, Camellia extract is a method of extracting by heating a dried camellia flower 5-20 times the volume of the solvent and heated for 3 to 20 hours at 50 ~ 100 ℃ and the dried camellia flowers at 1 to 15 at 5 to 37 ℃ Skin irritation emollient for cosmetics, characterized in that extracted by one or more methods selected from the method of immersion in a daily solvent extraction. The cosmetic skin stimulant according to claim 2, wherein the extract obtained by the method according to claim 2 is further fractionated with hexane, methylene chloride and n-butanol, and then obtained n-butanol fraction. . The method of claim 1 wherein the skin irritation relief is a skin irritant for cosmetics, characterized in that the anti-inflammatory in the skin. The method of claim 1, wherein the skin irritation relief is a skin irritant for cosmetics, characterized in that the anti-allergic in the skin. The method of claim 1, wherein the skin irritant is selected from a lotion, nutrition lotion, nutrition cream, massage cream, nutrition essence, emulsifying foundation, sunscreen lotion, sunscreen cream, baby bath cleaner, shampoo and soap. Skin irritant for cosmetics. The cosmetic skin irritant according to claim 1, wherein the camellia extract contains 0.01 to 10.0% (w / v) as dry weight.