KR100564107B1 - Cosmetic composition comprising plant extract having anti-aging effect - Google Patents

Abstract

The present invention relates to an anti-aging cosmetic composition containing a mixed plant extract, and more particularly, to an anti-aging cosmetic composition containing a mixed extract of Angelica, Chemo, Licorice, Schisandra chinensis and Baekjak.

The mixed plant extract of the present invention increases the collagen synthesis, inhibits the free radical scavenging antioxidant activity and elastase activity, thereby increasing skin elasticity, thus effectively preventing skin aging, improving skin elasticity, and reducing wrinkles. Can be used.

Anti-aging, Angelica, Cheongung, Licorice, Schisandra, Earl, Cosmetics

Description

Cosmetic composition containing mixed plant extract having anti-aging effect

The present invention relates to a cosmetic composition for preventing skin aging, and more particularly, to a cosmetic composition containing a mixed plant extract of Angelica, cheonsugung, licorice, Schisandra chinensis, Baekjakwan, having a free radical scavenging action, an antioxidant action and a wrinkle reducing action. .

One of the causes of aging is free, caused by free radicals such as superoxide anion radicals, hydroxy radicals, singlet oxygen and hydrogen peroxide (H ₂ O ₂ ) derived from oxygen. Radical reactions destroy major macromolecules in cells, including lipids, which are known to be highly reactive and cause abnormal metabolism by reacting with cellular components or cell membranes. Such abnormal metabolism, exposure to physical and chemical external stimuli can lead to imbalances in oxidation and antioxidants, resulting in peroxidation or incapacity of defense mechanisms in vivo, oxidation and degeneration of proteins, impaired cell function, Eventually, skin aging, characterized by decreased elasticity, wrinkles, blemishes and freckles, is accelerated ( J. Soc., Cosmet. Sci. Kor. , 23 , pp75-132, 1997). In particular, one of the many causes of aging and disease in living organisms can be found in active oxygen, which occurs during oxidative metabolism, and these are found in healthy tissues by white blood cells. Although it is essential for nonspecific removal of non-magnetic substances, it is known to cause peroxidation of lipids mainly by taking part in free radical reaction in biological membranes rich in unsaturated fatty acids. Although there are known active oxygen inhibitory and antioxidant substances used as raw materials for general cosmetics, medicines and foods, this is a synthetic product and its use is limited because it is not secured when applied to human skin for a long time. Natural products are mostly weak in inhibiting free radicals, but the safety of human skin is guaranteed. Therefore, a substance having strong active oxygen inhibitory effect and antioxidant effect and having another effect on human skin is desired.

A representative histological change in light aging is the accumulation of elastin, the main component of elastin fibers. Unlike normal collagen fibers, solar elastotic material is composed of macromolecules and severely impaired function by light. Elastin gene expression is markedly promoted in UV-exposed skin cells, and elastin gene expression is induced by free radicals. Elastase is the only enzyme known so far to break down elastin, and its inhibition can fundamentally reduce skin aging.

Degradation of collagen is mainly due to light. In particular, the accumulation of elastic material is a phenomenon caused by the simultaneous destruction of the surrounding collagen bundle, and the evidence can be confirmed by the presence of proteolytic enzymes responsible for the decomposition of extracellular matrix (ECM) in light aging. Most of these proteases can break down collagen, elastin fibers, and other dermal components. Photoaging is initiated by UV signal transmission, and skin aging can be prevented by retinoids and protein inhibitors that inhibit the expression of antioxidants or proteolytic enzymes that can initially block this. Is proved.

Looking at the mechanism of skin aging, first, free radicals or free radicals are generated by ultraviolet rays or external environmental factors, and cell membranes are attacked by the generated free radicals or free radicals, resulting in inflammation of the skin. Inflammated tissue destroys proteins and genetic material by physiological progression mechanisms. Skin aging that causes wrinkles or the like occurs. Therefore, inhibiting and preventing all steps involved in the skin aging mechanism can fundamentally prevent skin aging. Due to the disadvantage that the raw material having a conventional anti-aging effect is not fundamentally inhibited, research has been attempted to find new functional materials. Various natural natural products, which have been conventionally used as herbal medicines, have not only been excellent in safety but also contain various beneficial drug substances, and thus have been good search targets.

Angelica gigas Nakai is a naturalized plant grown in Korea. It is a perennial herb, native to Japan, 60 ~ 90cm high. Flowers are white and hang at the end of branches in August-September. Fruits are long, long oval, with narrow wings at the edges. The root is called Angelica, and it has the effect of blood, so there is no blood on the face, dizziness is frequent, and it is effective for people who do not have gloss on eyes and lips. Headache is weak body, women's menstrual control effect is excellent, so it is often used in various prenatal and postpartum diseases, and it treats menstrual irregularities and abdominal pain. It is effective in treating constipation due to weak gastrointestinal function (Bae Gi-hwan, color guidebook for health guidelines according to folk remedies and herbal remedies, pp102 ~ 103, 2003).

Cnidium officinale MAKINO is a perennial plant of the family Apiaceae, native to China and grown as a medicinal crop. Its height is 30-60 cm and its components are known as cnidilide, ligustilide, neocnidilide, butylphthalide and sedanonic acid. Cheonggung was used to dry the root stem in oriental medicine and used for gynecological diseases such as uterine sedation, blood pressure control, antibacterial action, inhibitory effect on dermatophytes, headache, anemia, infertility, dysmenorrhea and coldness. It was put in the closet for some prevention in the private sector (Information, Shin Min-kyo, Ambassador of Yakyang, pp418, 1998).

Licorice ( Glycyrrhiza uralensis FISCH.) Is a perennial herb, 30-80 cm tall. The shell is thin and reddish. Straight, long, very sweet. In addition, the curved side should have hard yellow fiber. The main components of licorice contain licorice saponin, flavonoids and glycyrrhizin. Roots and rhizomes have 5 to 14% glycyrrhizin and glabraic acid, which are very helpful for cough medicine, phlegm and bronchial asthma. In addition, more than 27 flavonoid-like substances have been identified by chromatograph in licorice root and used for myrrh, anti-inflammatory and anti-ulcer activity. It is also used for gastroduodenal ulcers, jaundice, eczema and other skin diseases. In addition, the pain caused by rapid tension of muscles, urgent symptoms are solved, detoxification, antitussive, expectorant action. In motion therapy, the roots are used mainly for the purpose of alleviating and harmonizing the actions of other medicines (interest, ingredients and use of herbs, pp387 ~ 393, 1999; Seung-hyun Roh, Hwang-in Hwang, TV Herb Kang, p297, 1999).

Schizandra chinensis Baillon (Schisandra chinensis Baillon.) Is a deciduous vine that grows in the mountains. The leaves are regenerated, ovate, serrated at the edges and hairy on the back. Fruits have red-fuchsia-black berries and net-like wrinkles and are used for borrowing or medicinal use as a unique scent. Schizandra chinensis fruits include essential oils, fatty oils, vegetable acids, and pigments. Fruit acts on carbohydrate metabolism, increases blood sugar and lactic acid, increases blood phosphate and decreases calcium (Ca). Schisandra chinensis fruit is an excellent herb that coughs, strengthens the liver, strengthens mental power, and improves memory (Han Yongbong, Korean Wild Plant Resources II, pp230 ~ 237, Choi Jin-gyu, Korean Herb, Flower * Tree, 1, p34, 2001).

It is a perennial plant, the root of persimmon flower of Paeonia japonica , which is less than 50cm in height and blooms in May-June. Dig in the fall, remove the rhizome, remove the beard root, rinse clean and dry. The root contains paeoniflorin, and the content is 1.8-7.3%, and the root bark contains 1-1.1%. In addition, it contains essential oils, fatty oils, resins, tannins, sugars, semen, etc., and contains 0.92% of aromatic acid. Earl of Paeonniflorin is effective in suppressing analgesic, anti-inflammatory, fungicidal, dysmenorrhea, etc. (Information Sup, Shin Mingyo, Hyangjeomsa, Yeonglimsa pp524 ~ 525, 1998).

Recently, in order to develop a relatively low toxicity and nature-friendly cosmetics, it has been discovered that several plants are effective in preventing skin aging and wrinkles by searching for plants having radical scavenging action. As an example, Korean Patent Publication No. 1994-35688 discloses a radical scavenger containing an extract of three hundred sec, and Korean Patent Publication No. 1994-36642 discloses a radical scavenger containing an extract of a plant of the genus Pheasant. , Korean Patent Publication No. 1997-58952 discloses skin wrinkle improvement cosmetics containing Yulpi extract, and Korean Patent Publication No. 2002-44266 discloses anti-aging of skin containing 38 herbal extracts such as Megmun-dong, health and root skin And cosmetic compositions for whitening, etc. have been disclosed, but to date, there is no known anti-aging and wrinkle-relieving action of the composition containing the extracts of Angelica, Cheongo, Licorice, Schisandra chinensis and Baekjak.

The present inventors studied skin aging, skin elasticity, and antiwrinkle effect on various herbal medicines, and found collagen synthesis, free radical scavenging activity and elastase inhibitory effect in the mixed plant extract of the present invention. The present invention was completed by confirming that it is effective in preventing skin aging.

It is an object of the present invention to provide a cosmetic composition comprising a mixed plant extract having an excellent anti-aging effect and an increase in skin elasticity as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin aging containing a mixed plant extracts of Angelica, cheonggung, licorice, Schisandra chinensis and Earl as active ingredients.

The mixed plant extract is Angelica: Cheongung: Licorice: Licorice: Schisandra chinensis: 1 ~ 25: 1 ~ 30: 1 ~ 30: 1 ~ 25: 1 ~ 25 dry weight ratio, preferably 10 ~ 25: 15 ~ 20 : 10 ~ 30: 10 ~ 25: 15 ~ 25 Include dry weight ratio.

The mixed plant extract may be used in the preparation of the extract by mixing the herbal medicine in the above ratio, it is also possible to prepare the extract in each plant and mix it in the dry weight ratio of the above.

The mixed plant extract is characterized in that it contains 0.001 to 90% by weight, preferably 0.5 to 50% by weight relative to the total weight of the composition.

Hereinafter, the present invention will be described in detail.

The mixed plant extract of the present invention, the dried Angelica, Cheongung, Licorice, Schisandra chinensis and Earl and completely cut or pulverized, to each ground or 1 to 25% by weight of Angelica, 1 to 20% by weight, Licorice 1-30 To a mixture of 1% to 25% by weight of Schizandra chinensis and 1 to 25% by weight of Earl, about 1 to 30 times the weight (kg), preferably about 10 to 20 times water, C ₁ to C ₄ Lower alcohol, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof, preferably distilled water is added, and cold washing, hot water extraction, ultrasonic extraction, reflux for about 0.5 hours to 15 days at an extraction temperature of 0 ℃ to 100 ℃ Extraction is performed using a cold extraction method, and the extract thus obtained may be filtered, concentrated under reduced pressure, or dried to prepare an extract.

In the above preferred extraction method, the cooling condenser is installed to prevent extraction of the solvent by evaporation at 50 to 95 ℃, 4 to 20 hours of heating extraction or 5 to 37 ℃ 1 to 15 days to extract the active ingredient The method may be used, and the mixed plant extract thus extracted may be used by filtration or may be concentrated and completely concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser.

The mixed plant extract of the present invention prepared above is preferably an extract containing a certain solid content, significantly increasing collagen synthesis in collagen biosynthesis, excellent free radical scavenging activity, and inhibiting elastase enzyme activity. It has an excellent effect to prevent skin aging, and has excellent skin elasticity and skin improvement effect when applied to the skin, and can be usefully used as a cosmetic composition for preventing skin aging.

The present invention contains a mixed plant extract of Angelica, Chemo, Licorice, Schisandra chinensis and Earl Tooth in an amount of 0.001 to 90% by weight, preferably 0.5 to 50% by weight of the total cosmetic composition. It provides an anti-aging cosmetic composition mixed with a base applicable as.

In addition, the cosmetic composition of the present invention can be applied and blended as necessary components, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., which are formulated in general skin cosmetics. Do.

Cosmetic compositions containing the mixed plant extract of the present invention as an active ingredient may be used in various ways, such as anti-aging cosmetics, face wash, body cosmetics.

Examples of products to which the present composition can be added include cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and cleansing agents, face washes, soaps, treatments, and essences. .

Specific examples of the cosmetic of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, essence, nutrition essence, pack, soap, Cleansing foam, cleansing lotion, cleansing cream, body cleanser, shampoo, latex, foundation, press powder, loose powder, eye shadow and the like.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example 1 Preparation of Mixed Plant Extract 1

1kg of mixed dried Angelica 20%, 20% Cheongung, 20% Licorice, 20% Schisandra chinensis and 20% Baekja was added to 10kg of distilled water, extracted for 3 hours at 95 ℃ in an extractor equipped with a cooling condenser, and filtered through a 400 mesh filter cloth. After cooling to room temperature, the mixture was allowed to stand at 5-15 ° C. for 7 days to mature, and then filtered through Whatman No. 2 filter paper and then filtered through a 0.45 μm filter to obtain 8.8 kg of an extract having 3.5% solids.

Example 2-4. Manufacturer of mixed plant extracts 2-4

Extracted by the same method as in Example 1 except for the solvent conditions only, the yield and solid content are described in Table 1 below.

menstruum Yield (kg) Solid content (%) Example 2 30% hydrous ethyl alcohol 8.5 3.6 Example 3 30% water butylene glycol 8.6 3.5 Example 4 30% water propylene glycol 8.5 3.3

Example 5. Preparation of Mixed Plant Extract 5

1kg of mixed dried Angelica 20%, Cheongung 20%, Licorice 20%, Schisandra chinensis 20%, Baekjak 20% in 10kg of purified water, aged at room temperature for 7 days, filtered with 400 mesh cloth and filtered with 0.45㎛ filter, 3.5% 8.5 kg of an extract having a solid content of was obtained.

Example 6-8. Manufacturer of mixed plant extracts 6-8

Extracted by the same method as in Example 5 except for the solvent conditions only, the yield and solid content are described in Table 2 below.

menstruum Yield (kg) Solid content (%) Example 6 30% hydrous ethyl alcohol 8.3 2.3 Example 7 30% water butylene glycol 8.1 2.5 Example 8 30% water propylene glycol 8.2 2.5

Example 9 Preparation of Mixed Plant Extract 9

The dried dried Angelica 1kg 30% hydrated butylene glycol was put into 10kg aged for 7 days at room temperature and extracted, filtered through a 400 mesh filter cloth and filtered with a 0.45㎛ filter to obtain 8.6kg (solid content 2.9%).

Example 10. Preparation of Mixed Plant Extract 10

1 kg of fully dried Cheongung was put in 10 kg of 30% hydrous butylene glycol, aged at room temperature for 7 days, extracted, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to obtain 8.3 kg of an extract (1.9% solids).

Example 11 Preparation of Mixed Plant Extract 11

1 kg 30% of dried licorice was added to 10 kg of aqueous butylene glycol, aged for 7 days at room temperature, extracted, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to obtain 8.8 kg (3.3% solids) of the extract.

Example 12 Preparation of Mixed Plant Extract 12

1 kg of completely dried Schisandra chinensis was added to 10 kg of 30% hydrous butylene glycol, aged for 7 days at room temperature, extracted, filtered through a 400 mesh filter cloth and filtered through a 0.45 μm filter to obtain 8.8 kg (1.8% solids) of the extract.

Example 13. Preparation of Mixed Plant Extract 13

1 kg of completely dried white peony was added to 10 kg of 30% hydrous butylene glycol, aged for 7 days at room temperature, extracted, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to obtain 8.1 kg (2.3% solids) of the extract.

Experimental Example 1. Collagen Biosynthesis Test of Mixed Plant Extracts

Collagen synthesis amount was measured for the mixed plant extracts (Taubman, MB et al., Science 186 , pp1115, 1974).

The collagen synthesis amount was measured by PIP (Procollagen Type I C-peptide) EIA assay, and quantitatively measured the type I procollagen C terminal peptide (PICP) which is synthesized the most.

1 × 10 ⁴ cells were dispensed into 24 well plates and incubated for 24 hours. After incubation, the medium was replaced with a serum-free medium, and each sample was added at a concentration of 50 µg / ml and incubated in a 37 ° C., 5% CO ₂ incubator for 3 days. As a control, each well was treated with vitamin C, which is known to promote collagen synthesis, at a concentration of 50 µg / ml in each well. The culture supernatant medium was centrifuged and used for PIP (Procollagen Type I C-peptide) EIA KIT. In a 96 well plate coated with mouse monoclonal antibody to PIP, the antibody-POD conjugate (MK1O1, TAKARA BIO INC.) Labeled with peroxidase was added and reacted thereto, and the culture solution was added to 20 µl / well. Added.

After reacting for 3 hours at 37 ° C. in a 5% CO ₂ incubator, 100 μl / well of ρ-phenylenediamine, a substrate solution, was added and developed. After standing at room temperature for 15 minutes, the reaction was stopped by adding 100 µl / well of a solution for stopping the reaction. Absorbance was measured at 450 nm within 5 minutes, and the collagen content was measured by graphing the PICP standard concentration.

The results are shown in Table 3 below. The amount of collagen synthesized as a control vitamin C was 453 ng / ml, and the mixed plant extract of Examples of the present invention also showed a similar degree of collagen synthesis, and Examples 2 and 7 showed an excellent amount of collagen synthesis.

Determination of Collagen Synthesis of Mixed Plant Extracts sample Collagen Synthesis Amount (ng / ml) Control 453 Example 1 457 Example 2 509 Example 3 450 Example 4 482 Example 5 423 Example 6 451 Example 7 534 Example 8 479 Example 9 219 Example 10 364 Example 11 239 Example 12 295 Example 13 220

Experimental Example 2 Experiment of Cell Proliferation Effect of Mixed Plant Extract

The effect of cell proliferation was measured on the mixed plant extract (MTT assay, J. Immunological Methods 65 , p55, 1983).

MTT assay is a method widely used for cell proliferation or toxicity by measuring the number of living cells. The living cells are water-soluble and yellow salts of MTT (3- [4,5-dimethylthiazole-2-yl] -2,5 in the mitochondria. It uses the principle that -diphenyltetrazolium bromide is reduced to a water-insoluble blue formazan derivative by succinate dehydrogenase (also called succinate dehydrogenase or mitochondrial dehydrogenase). The resulting formazan derivative is dissolved by adding a dissolving agent (usually dimethylsulfoxide) and measuring the absorbance. In more detail, 100 μl of 5% fetal bovine serum (FBS) solution was added to a 96 microwell plate, and then the sample was dispersed in a predetermined concentration in DMEM medium containing 2% FBS, and diluted 100 μl was added again. Using a hemocytometer, fibroblasts obtained from neonatal foreskin tissue were dispersed in 100 μl medium per well and cultured for 7 days. After incubation, the supernatant was removed and washed again by adding 200 μl of 5% PBS (phosphate buffered saline), 100 μl of MTT solution was added per well, MTT was removed after 4 hours, and 100 μl of DMSO was added overnight, and then 37 ° C. overnight. Absorbance was measured at 570 nm after incubation at. At this time, the experiment was conducted in the same manner without adding the mixed plant extract as a control group, and the cell proliferation effect (%) compared with the control group was compared from the difference in relative absorbance.

As a result, the mixed plant extract of Examples of the present invention showed a cell proliferation effect, in particular, it was confirmed that the cell proliferation effect of Examples 7, 8 and 10 was excellent (see Table 4).

Cell Proliferation Effect of Mixed Plant Extracts      Example  Cell proliferation effect (%) Example 1 105 Example 2 113 Example 3 100 Example 4 108 Example 5 103 Example 6 100 Example 7 125 Example 8 117 Example 9 107 Example 10 116 Example 11 101 Example 12 109 Example 13 104

Experimental Example 3. Free radical scavenging ability test of mixed plant extract

For the mixed plant extract, using DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) according to Park's method (Park, S. N, Korean Society of Cosmetic Products, 23 (1) , pp75-132, 1997) The free radical scavenging effect was measured.

2 ml of the mixed plant extract was added to 1 ml of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution at each concentration, and reacted at room temperature for 10 minutes, and then absorbance was measured at 520 nm to free radical scavenging. The effect (%) was calculated | required. At this time, the control group used methanol instead of DPPH, and 2 ml of the plant mixture extract of the same concentration as the experimental group was added. In addition, as a positive control of the free radical scavenging ability, it was compared with the green tea extract containing a large amount of flavonoid components excellent in the free radical scavenging ability.

Free radical scavenging activity SC ₅₀ was expressed as the concentration (μg / ml) of the mixed plant extract required to remove 50% of the free radicals.

Looking at the results of Table 5, the mixed plant extracts of Examples 1 to 8 exhibited an SC ₅₀ concentration similar to the free radical scavenging ability of the green tea extract, it was confirmed that the free radical scavenging ability.

Free Radical Scavenging Effect of Mixed Plant Extracts Example SC ₅₀ (μg / ml) Green Tea Extract 89 Example 1 113 Example 2 97 Example 3 103 Example 4 98 Example 5 117 Example 6 108 Example 7 91 Example 8 96 Example 9 219 Example 10 325 Example 11 396 Example 12 271 Example 13 305

Experimental Example 4. Inhibitory effect of elastase activity of mixed plant extracts

Elastase inhibitory effect was measured on the mixed plant extract of the present invention (Lee et al. J. Cosmetic Science , 49 , pp 285-297, 1998).

In the experiments below, the buffer was prepared by adjusting 0.267M Tris solution with 0.267M hydrochloric acid (HCl) solution to pH 8.0, and the substrate solution was prepared by using the elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard 8.8. The one prepared in mM was used, and the enzyme prepared was prepared such that porcine pancreatic elastase standard product was 10 µg / ml.

In order to measure the elastase activity, 20 μl of substrate solution and 100 μl of sample (prepared by diluting the mixed plant extracts of Examples 1 to 13 in 0.1% of purified water) in 60 μl of buffer solution were added, and 20 μl of enzyme solution was added thereto. The mixture was shaken and reacted at 25 ° C. for 15 minutes, and the absorbance (B) was measured at a wavelength of 410 nm for the amount of p -nitroaniline.

In addition, the absorbance (C) was measured using 60 µl of the buffer solution, 20 µl of the substrate solution, 20 µl of purified water, and 100 µl of the sample.

Separately, the absorbance (A) was measured using 100 μl of purified water instead of the sample as the test solution, and the absorbance (A) was measured, and 120 μl of purified water was used instead of the enzyme solution and the sample solution. The absorbance (D) was measured as a color correction liquid, and the elastase inhibition rate (%) was calculated | required according to following formula (1), and the result is shown in Table 6.

% Elastase Inhibition = [1-(B-C) / (A-D)] × 100

Elastase Inhibitory Activity IC ₅₀ was expressed as the concentration (μg / ml) of the mixed plant extract required to inhibit 50% of the substrate of elastase.

Elastase Inhibitory Effects of Mixed Plant Extracts Example IC ₅₀ (μg / ml) Example 1 256 Example 2 311 Example 3 273 Example 4 298 Example 5 374 Example 6 287 Example 7 219 Example 8 266 Example 9 869 Example 10 958 Example 11 803 Example 12 543 Example 13 585

Preparation Example 1 Preparation of Lotion

An example of the preparation of the lotion in the cosmetic composition containing extracts from Angelica, Chemoung, Licorice, Schisandra chinensis and Earl of Japonica is as follows. The mixed plant extract used herein is that of Example 7.

number Raw material Formulation Example 1 Comparative Formulation Example 1 One   Mixed Plant Extract 50.0 - 2 Butylene glycol 7.0 7.0 3 glycerin 5.0 5.0 4 Polyoxyethylene (60) Cured Castor Oil 0.2 0.2 5 ethanol 5.0 5.0 6 Betaine 2.0 2.0 7 Citric acid 0.02 0.02 8 Sodium citrate 0.06 0.06 9 antiseptic a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount

<Production method>

2, 3, 4, 6, 7, 8, and 9 were added to 11 to dissolve and stirred (A), 10 times to 5 to dissolve, and this was added to A and stirred. To this was added 1 and stirred sufficiently before aging.

Preparation Example 2 Preparation of Lotion

An example of preparation of a lotion in a cosmetic preparation containing extracts from Angelica, Chemoung, Licorice, Schisandra chinensis and Earl of Japonica is as follows. The mixed plant extract used herein is that of Example 7.

number      Raw material Formulation Example 2 Comparative Formulation Example 2 One   Mixed Plant Extract 40.0 - 2 Butylene glycol  8.0  8.0 3 glycerin  5.0  5.0 4 Squalane 10.0 10.0 5 Butylene Glycol Dicaprylate / Dicaprate  5.0  5.0 6 Sorbitan stearate  1.5  1.5 7 Polysorbate 60  1.0  1.0 8 Glyceryl Stearate  0.5  0.5 9 Stearyl glycyrrhetinate  0.2  0.2 10 Carboxy Vinyl Polymer  0.1  0.1 11 Arginine  0.1  0.1 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Production method>

Heat 1, 2, 3, 11, 12, 14 to 70 ~ 75 ℃ while stirring and mix (A) Heat 4, 5, 6, 7, 8, 9 to 75 ~ 80 ℃ with stirring (B) After adding B to A to emulsify and cooling to about 45 ° C. while stirring, 10 and 13 times are added and stirred, and then cooled to 30 ° C. and aged.

Preparation Example 3 Preparation of Essence

An example of the preparation of the essence in the cosmetic composition containing extracts from Angelica, Chemoung, Licorice, Schisandra chinensis and Earl of Peony is as follows. The mixed plant extract used herein is that of Example 7.

number Raw material Formulation Example 3 Comparative Formulation Example 3 One   Mixed Plant Extract 10.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Carboxy Vinyl Polymer 0.2 0.2 10 Xanthan Gum 0.2 0.2 11 antiseptic a very small amount a very small amount 12 Spices a very small amount a very small amount 13 Purified water Remaining amount Remaining amount

<Manufacturing method>

2, 3, 4, 5, 6 times (A) are stirred and mixed, and 1, 7, 8, 13 times (B) are separately stirred and mixed. When B was added to E to emulsify and cooled to about 45 ° C. while stirring, 12 times was added and stirred, then cooled to 30 ° C. and aged. Add 9, 10, 11 to disperse, stabilize and mature.

Preparation Example 4 Preparation of Facial Cleanser (Cleansing Foam)

An example of the preparation of a facial cleanser among cosmetics containing extracts from Angelica gigas, Cheongung, Licorice, Schisandra chinensis and Earl of Peony is as follows. The mixed plant extract used herein is that of Example 7.

number      Raw material Formulation Example 4 Comparative Formulation Example 4 One  Mixed Plant Extract 20.0 - 2  N-acyl glutamate 20.0 20.0 3  glycerin 10.0 10.0 4  PEG-400 15.0 15.0 5  Propylene glycol 10.0 10.0 6  POE (15) oleyl alcohol ether 3.0 3.0 7  Lauric derivatives 2.0 2.0 8  Methyl Paraben 0.2 0.2 9  EDTA-4Na 0.03 0.03 11  Spices 0.2 0.2 12  Purified water Remaining amount Remaining amount

<Manufacturing method>

Mix 12, 3, 4, and 5, and add 1 small portion. It heat-dissolves at 80 degreeC, stirring 9 times, (A), and 6, 7, 8 (B) is melt | dissolved at 80 degreeC separately, and is put in A. After stirring and mixing, 11 was added and cooled slowly.

Preparation Example 5 Preparation of Shampoo

Examples of the preparation of shampoos in cosmetics containing extracts from Angelica, Chemoung, Licorice, Schisandra chinensis and Earl of Peony are as follows. The mixed plant extract used herein is that of Example 7.

number      Raw material Formulation Example 4 Comparative Formulation Example 4 One  Mixed Plant Extract 20.0 - 2  TEA Lauryl Sulfate 20.0 20.0 3  Sodium Lauryl Ether Sulfate 30.0 30.0 4  Lauryl Amidopropyl Betaine 2.0 2.0 5  Lauryl acid diethanol amide 3.0 3.0 6  glycerin 2.0 52.0 7  EDTA-2Na 0.05 0.05 8  Methyl Paraben 0.2 0.2 9  Citric acid 0.03 0.03 10  Zinc pyrithione 0.02 0.02 11  Spices 0.2 0.2 12  Purified water Remaining amount Remaining amount

<Manufacturing method>

6 and 7 were mixed in 12 times, and it heat-dissolved at 80 degreeC, and 2, 3, 4, 5, and 6 times were added and stirred. After confirming dissolution, neutralize by adding 9 times at 50 ° C, and mixing 1 and 10 times at 45 ° C with stirring.

Test Example 1 Anti-Aging Confirmation Test on Skin: Increased Elasticity Test on Skin

In order to measure the effect of increasing the elasticity of the skin when the lotions of Formulation Example 2 and Comparative Formulation Example 2 were applied to the skin, the left side of the face of 20 women aged 20 to 55 years of age had normal, oily, dry and combination skin. Part of the lotion of Formulation Example 2, and the lotion of Comparative Formulation Example 2 on the right side of the face was applied twice a day in the morning and evening, skin elasticity measuring device after 1 week, 2 weeks, 3 weeks and 4 weeks The elasticity of the skin was measured using a (Ballistometer).

The measurement results are shown in Table 12 below.

Cosmetic formulation of Formulation Example 2 Cosmetics of Comparative Formulation Example 2 T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Amplitude first 1.6 3.8 5.3 5.6 4.9 1.1 2.8 3.0 3.4 3.1 second 1.5 3.6 5.1 5.0 4.1 1.3 2.3 2.0 2.1 2.6 third 0.0 2.4 4.3 3.5 3.0 0.0 0.0 1.6 2.1 1.8 fourth 0.0 1.0 3.5 3.6 3.1 0.0 0.0 0.0 0.0 0.0 Average 0.78 2.70 4.55 4.42 3.77 0.60 1.27 1.65 1.90 1.88 (Note) T ₀ ; Before use T ₁ ; After 1 week of use T ₂ ; After 2 weeks of use T ₃ ; After 3 weeks of use T ₄ ; After 4 weeks

As can be seen from the above results, the lotion of Formulation Example 2 containing the mixed plant extract according to the present invention, compared to the lotion of Comparative Formulation Example 2, which was prepared without addition, has a very good skin elasticity effect by the skin condition improvement effect. Able to know.

Experimental Example 2. Experiment to confirm the effect of improving skin condition

In order to confirm the skin condition improvement effect during the skin coating on the essence of Formulation Example 3 and Comparative Formulation Example 3, the following clinical confirmation experiment was carried out. Fifty-five owners of normal, oily, dry, and complex skin were composed of 25% of women aged 20-55 years old to investigate the improvement of skin condition and the skin's oil and moisture status. To the same person, the essence of Formulation Example 3 was applied to the upper part of the face, and the essence of Comparative Formulation Example 3 was applied to the skin once a day for 20 days once a day after washing the face every morning. Was measured. The test results are shown in Table 13.

Survey Items Skin condition improvement Number of people % Formulation Example 3 very good 18 36.0 good 22 44.0 is average 10 20.0 so so - - Comparative Formulation Example 3 very good - - good 10 20.0 is average 17 34.0 so so 23 46.0

As described above, the cosmetic composition containing the mixed plant extracts of Angelica, cheonggung, licorice, Schisandra chinensis and Earl of the present invention increases collagen biosynthesis, has an effect on cell proliferation, eliminates free radicals and acts as an antioxidant to elasta Since it is effective in preventing aging of the skin by inhibiting the active agent, it may be usefully used as a cosmetic for preventing skin aging, improving skin elasticity, and reducing wrinkles.

Claims (6)

A skin composition for preventing skin aging containing a mixed plant extract in which the mixing ratio of Angelica, Cheongung, Licorice, Schisandra chinensis and Earl of Peony is 10-25: 15-20: 10-30: 10-25: 15-25. delete delete The cosmetic composition according to claim 1, wherein the extract is extracted using water, C ₁ to C ₄ lower alcohols, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof as an extraction solvent. The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap Cosmetic composition, characterized in that it has any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, shampoo, latex, foundation, press powder, loose powder, eye shadow.