KR100773406B1 - Crude drug composition showing anti-oxidative, anti-tumor and anti-bacterial activity - Google Patents

Abstract

The present invention relates to a composition containing a mixed herbal extract including gold silver, red peony, saenggihwang, cheonggung, donggi, rhubarb, wild gardenia, yeonkyo and licorice, specifically, the extract of the present invention is an antioxidant identified by the electron donating ability and SOD-like activity It shows the effect, growth inhibitory effect on cancer cell lines and growth inhibitory activity against skin flora and oral bacterial strains can be usefully used as a cosmetic composition for antioxidant, anticancer and antibacterial.

Geumgumhwa, red peony, saengjihwang, Cheongung, Angelica, Huang lotus, wild gardenia, kyokyo, licorice, antioxidant, anticancer, antibacterial, cosmetic composition

Description

CHH Crude drug composition showing anti-oxidative, anti-tumor and anti-bacterial activity}

The present invention relates to a composition containing a mixed herbal extract comprising antioxidation, anticancer and antibacterial activity, including silver coins, red peony, saengjihwang, Cheongung, Angelica, rhubarb, wild gardenia, yeonkyo and licorice.

Human skin is constantly changing, the most representative of which is deterioration of the skin function due to aging and reduction of visual beauty. Skin aging is classified into internal aging due to genetic factors and external aging due to external environmental factors such as sunlight (Doris EV, Cosm. & Toil. USA , 111 , pp31-37, 1996). External aging causes wrinkles on the skin, and the representative factors for wrinkle formation include reduction of biosynthesis of free radicals, ultraviolet rays and collagen. In the case of internal aging of the skin, artificial control is impossible, but in the case of external aging, it is easier than internal factors to control the removal of free radicals, the proliferation of fibroblasts and the promotion of collagen biosynthesis, among which vitamin A and its derivatives are It is known to alleviate wrinkles by promoting fibroblast proliferation and collagen synthesis. In addition, sunlight and the like in the skin not only form wrinkles but also stimulate melanocytes, which are pigment cells present in the base layer, to synthesize melanin. This overproduction of melanin forms spots and freckles and is also closely related to skin cancer. Tyrosinase is an enzyme involved in the biosynthesis of melanin in melanocytes, and cinnamic acid, benzoic acid, cysteine, and chlorogenic acid are known as inhibitors of tyrosinase. In addition, hydroquinone (melanostatin), tropolone, kojic acid and arbutin have been used, including hydroquinone, which is mainly used to treat women's blemishes, but has safety problems. In many cases, the effects are not clearly or clearly identified. Therefore, attempts have been made to use a substance having excellent tyrosinase inhibitory ability from natural products that humans have eaten safely for a long time.

Antioxidant activity refers to an activity that prevents the production of free radicals in vivo and prevents oxidative phenomena that cause irreparable damage to cells. Steady state oxygen (triplet oxygen) is a superoxide radical, hydroxyl radical, hydrogen peroxide (H ₂ O ₂ ) due to environmental and biochemical factors such as enzymes, reduction metabolism, chemicals, pollutants, photochemical reactions, etc. It is known to convert into reactive oxygen species (ROS), such as ROS, to oxidize various cell components, such as lipids, proteins, and DNA, causing inflammation or damaging various organs (Beckman JS, et al., Proc) . Natl.Acad . Sci. USA , 87 , pp 1620-1624, 1990: Sagar S, et al., Mol. Cell Biochem., 111, pp 103-108, 1992: Ames BN, et al., Proc. Natl. Acad. , USA, 90, pp 7815_7922, 1993). Examples include superoxide anions ( ¹ O ₂ ), hydrogen peroxide, hydroxyl radicals, singlet oxygen ( ¹ O ₂ ), and alkoxyl radicals (RO ·) produced by lipid peroxidation. , Peroxyl radical (ROO ·) and nitrogen peroxide ion (ONOO ⁻ ).

The action of these free radicals affects the action of antioxidant enzymes such as superoxide dismutase (SOD), catalase, and peroxidase, and the antioxidants such as vitamins C, E, and glutathione. However, if the biodefense is abnormal or exposed to excessive free radicals, the balance is broken, and the free radicals irreversibly destroy substrates, proteins, DNA, etc., and as a result, aging , Cancer, multiple atherosclerosis, arthritis, and parkinson's disease have been reported to cause a variety of diseases (Griffiths HR, et al., FEBS Lett ., 388 , pp161-164, 1996; Squadrito GL, et al., Free Radic. Biol. Med ., 25 , pp392-493, 1998; Choi JS, Phytochem.Res ., 16 , pp232-235, 2002; Dreher D, et al., Eur. J. Cancer , 32A (1), pp 30-38, 1996; Sohal RS, et al., Free Radic. Biol. Med ., 33 (1) , pp 37-44, 2002).

In order to prevent diseases caused by oxidative stress and prevent oxidative deterioration of foods, many studies are being conducted to isolate, identify and develop powerful and harmless natural antioxidants from edible or medicinal plants. Plant-derived natural compounds are also reported to retard the oxidation process as free radicals and scavengers or reducing agents of reactive oxygen species (Pratt, DE, American Chemical Society , pp 54-71, 1994: Larson, RA, Phytochem ., 27 , pp 969-978, 1988).

Synthetic antioxidants developed so far include butylated hydroxy anisole (BHA), butylated hydroxy toluene (BHT) and nordihydro-guaiaretic acid (NDGA) .Natural antioxidants include superoxide dismutase, peroxidase, catalase, and glutathione peroxide. Antioxidant enzymes such as oxidase and non-enzymatic antioxidants such as tocopherol (vitamin E), vitamin C (vitamin C, ascorbic acid), carotenoids and glutathione.

However, synthetic antioxidants are toxic in vivo and can cause allergies and tumors. On the other hand, natural antioxidants have the advantage of being safe to the living body compared to synthetic antioxidants, but the disadvantage is that the effect is weak. Therefore, there is an urgent need for the development of new natural antioxidants with excellent antioxidant activity and safety. In the DPPH radical scavenging effect test used to confirm the antioxidant effect, DPPH is a stable free radical, exhibiting a maximum absorption near 517 nm due to its non-covalent electrons. The high ability to reduce or offset can be expected to have high antioxidant activity and scavenging activity against other radicals, including active oxygen.

In addition, cancer is one of the incurable diseases to be solved by humankind, and huge capital is being invested in the development to cure it all over the world. It is diagnosed, and about 60,000 or more are dead. Carcinogens, which cause cancer, are smoking, ultraviolet rays, chemicals, foods, and other environmental factors. However, various causes are not only difficult to develop a therapeutic agent, but also effective effects vary depending on the site of occurrence. Currently, the substances used as therapeutic agents are extremely toxic and cannot selectively remove only cancer cells. Therefore, there is an urgent need for the development of low-toxic and effective anti-cancer agents to prevent cancer after treatment. .

Mixed herbal remedy of the present invention is a prescription for the heat disinfection sound of the prescription contained in the synonym Bogam is a complex prescription medicine of Onggi is related to purulent inflammatory disease occurring deep in the tissue around the hair follicles, it is formed by the aid of the aid and blood vessels are congested and meridians are blocked. Clinically, hard and painful red nodules appear in the hair, and Western medicine is believed to be caused by microorganisms, physicochemical factors, and circulatory disorders. The medicinal compounds of the sound of heat disinfection are the effect of Lonicera japonica, Forsythiae Fructus, Coptis chinensis and Cyanide which are effective in antibacterial, anti-toxic, detoxification and anti-inflammatory. , Red Peony (Paeonia lactiflora.PALL) and Rhenanniae Radix, which are effective in treating heat loss of nutrition and blood powder, Glycyrrhiza uralensis Fischer It is composed of Angelica gigas Nakai , which improves or eliminates bloody symptoms, and Cnidium officinale Makino, which promotes blood circulation and releases fish. Has been used.

Although research on analgesic and antithrombotic effects has been reported as a study on the heat disinfection sound, there are many studies on the anti-cancerous sound and component drugs that can be used as natural materials, clinical studies, enzymatic aspects of antioxidant effects, and anti-cancer and antibacterial effects. No studies have been reported.

The present invention is composed of herbal heat disinfection sound as prescribed in Dongbobom while continuing to find a raw material with excellent stability and antioxidant, anti-cancer and antibacterial effect among herbal extracts used in herbal medicine, vegetables, fruits, flowers, etc. When applied to the gold and silver coins, red peony, saengjihwang, Cheonggung, Angelica, Huangyan, wild gardenia, yeonkyo and licorice at the same time showed excellent antioxidant, anticancer and antimicrobial effect confirmed the applicability as a cosmetic natural material and completed the present invention.

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic composition containing a mixed herbal extract including gold and silver, red peony, saenggihwang, cheonggung, donkey, rhubarb, wild gardenia, yeonkyo and licorice exhibiting anti-oxidant, anticancer and antimicrobial effects and having no stability problems. .

In order to achieve the above object, the present invention provides a composition containing a mixed herbal extract, including gold and silver, red peony, saenggihwang, cheonggung, angwi, rhubarb, wild gardenia, yeonkyo and licorice having antioxidant, anticancer and antibacterial effects.

Preferred mixed dry weight ratio (w / w) of the extract of the present invention is 1 ~ 15 gold silver coins: 1 ~ 10 red jeokpuk 1 ~ 10: Saenggwanghwang 1 ~ 10: Cheongung 1 ~ 10: Angelica 1 ~ 5: Yellow lotus 1 ~ 5: Gardenia 1 ~ 5: Yeongyo 1-5: Licorice 1-5, more preferably Geumeunhwa 1 ~ 10: Red medicine 1-7: Saenghwanghwang 1-7: Cheongung 1-7: Angelica 1-4: Huangyan 1-4: Sanchija 1-4: Yeonkyo 1-4: Licorice 1-4, even more preferably gold and silver 1-5: Red-eye 1 ~ 3: Raw yellow sulfur 1 ~ 3: Cheongung 1 ~ 3: Angelica 1: Huang Yan 1: Sanchija 1: Yeongyo 1: Mixed with licorice 1 will be.

The extract includes water containing purified water, a lower alcohol having 1 to 4 carbon atoms or propylene glycol, an extract available in at least one solvent selected from the group consisting of ethyl acetate, butylene glycol, ether, chloroform.

Hereinafter, the present invention will be described in detail.

The extract of the present invention is 1 to 15 gold, 1 to 10, red pepper 1 to 10, 1 to 10, guinea pig 1 to 10, Angelica 1 to 5, 1 to 5, rhubarb 1 to 5, Fructus 1 to 5, lichen 1 to 5, licorice 1 to 5, Preferably, 1 to 10 gold, 1 to 7, red tide 1 to 7, 7, 1 to 7, Saenggi 1 to 7, Angelica 1 to 4, 1 to 4, Gardenia 1 to 4, soft bridge 1 to 4 and licorice 1 to 4 dry weight ratio ( w / w), and then, in an extraction solvent, ethanol 1 to 30 times, preferably 5 to 15 times the volume of 10 to 50 ℃, preferably 0.5 to 48 hours at room temperature, preferably 20 to 30 Extraction method such as cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction for a time, preferably by cold extraction or distilled water 1 to 30 times, preferably 5 to 15 times the volume of the extraction solvent Within 0.5 at an extraction temperature of 30 to 100 ° C. and preferably 60 to 90 ° C. Extraction method of extraction for 10 hours, preferably 1 to 5 hours, cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, preferably reflux cooling extraction, the extract is centrifuged, filtered, concentrated To obtain.

 The composition of the present invention comprises 0.01 to 10% by weight, preferably 0.1 to 8% by weight, most preferably 0.5 to 5% by weight, based on the total weight of the composition.

As a result of measuring the electron donating ability of the extract obtained by the extraction method, the antioxidant effect confirmed by the SOD-like activity, the growth inhibitory effect on cancer cell lines and the growth inhibitory activity against skin flora and oral bacterial strains, The anti-proliferative effect and the antimicrobial effect against skin flora and oral bacterial strains can be usefully used as a cosmetic composition for antioxidant, anticancer and antibacterial.

In addition, the present invention provides a cosmetic composition for antioxidant, anticancer and antimicrobial containing a mixed herbal extract including gold and silver, red peony, raw yellow sulfur, cheonggung, Angelica, rhubarb, wild gardenia, yeonkyo and licorice obtained in the extraction process.

The composition containing the extract of the present invention may be used in various ways, such as cosmetics and face wash for the anti-aging, cancer prevention and antibacterial effect of the skin. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments, such as a silk powder, a cellulose, CI pigment yellow, and CI pigment orange, and the composite pigment of these inorganic pigments and an organic pigment, etc. are mentioned.

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably from 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Functional cosmetic composition for antioxidant, anticancer and antimicrobial of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics, etc. Can be mentioned.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Reference Example 1. Preparation of Experimental Materials

The herbal remedies used in this experiment were based on the contents recorded in the agreement.

Example 1. Preparation of CH Extract

1-1. Water extract manufacturer

The medicinal herbs of the present invention were mixed with gold and silver coins 7.5, red peas 5.625, raw green sulfur 5.625, Cheongung 5.625, Angelica 3.750, rhubarb 3.750, Sanchija 3.750, Yeongyo 3.750, licorice 3.750% by weight, and distilled water of 10 times the total weight was added at 85 ° C. Extracted by reflux cooling for 3 hours, the supernatant and precipitate were separated and extracted three times to obtain a water extract, followed by centrifugation, filtration and concentration, and freeze-dried and stored in a refrigerator compartment (hereinafter referred to as CHW name). Business card).

1-2. Ethanol extract manufacturer

The medicinal herbs of the present invention were mixed with Geumgumhwa 7.5, Red Peony 5.625, Saenghwanghwang 5.625, Cheongung 5.625, Angelica 3.750, Huangyan 3.750, Sanchija 3.750, Yeongyo 3.750, Licorice 3.750 wt%, and added 70% ethanol, 10 times the total weight, to room temperature. After immersing in the supernatant for 24 hours, the supernatant was separated from the precipitate, and the supernatant was taken. The extract was repeated three times in the same manner to obtain an ethanol extract, followed by centrifugation, filtration and concentration, and stored in a refrigerating chamber after freeze-drying. (Hereinafter referred to as CHE).

Example 2 Preparation of Skin Lotion

Skin lotions were prepared using the extract of CH.

number Raw material weight(%) One   CH extract 1.0 2 Butylene glycol 5.0 3 glycerin 3.0 4 Carboxy Vinyl Polymer 0.06 5 Disodium Ethylenediaminetetraacetic Acid 0.01 6 ethanol 7.0 7 Sorbitan monostearate 0.8 8 Triethanolamine 0.2 9 Hydrogenated Castor Oil 0.4 10 Phenyltrimethicone 0.5 11 Paraoxybenzoic acid propyl 0.2 12 Methyl paraoxybenzoate 0.2 13 Spices 0.1 14 Purified water Remaining amount system 100.0

Experimental Example 1. Determination of antioxidant effect of blue heat disinfection sound and constituents extract

1-1. Electronic donation ability test

Electron donating ability (EDA) of CH extract was performed by modifying Blois' method. (Doris EV, Cosm. & Toil. USA , 111 , pp31-37, 1996)

1.0 mL of 0.2 mM DPPH (α-α-diphenyl-β-picrylhydrazyl) was added to 2.0 mL of each sample solution, stirred for 30 minutes, and then absorbance was measured at 517 nm. The electron donating ability is expressed as the absorbance decrease rate of the experimental and control groups of the sample solution, shown in Table 1 below.

sample Sample concentration (µg / mL) Radical scavenging effect (%) CHW   One 5.4 ± 1.9 10 16.9 ± 1.4 100 61.6 ± 2.5 1000 64.2 ± 0.5 CHE One 4.5 ± 2.1 10 28.4 ± 2.6 100 80.3 ± 1.0 1000 87.1 ± 2.0

Experimental results showed that the electron donating ability increased depending on the concentration of the extract, and the ethanol extract of the mixed herbal showed an excellent electron donating ability of more than 80% at a concentration of 100 µg / ml or more. The electron donating ability showed the excellent antioxidant effect.

1-2. SOD Pseudo-activity Verification test

SOD-like activity was performed according to Marklund's method. (Marklund S, Marklund G., Eur. J. Biochem ., 47 (3), pp469-474, 1974)

To 0.2 ml of each sample solution, 2.6 ml of Tris-HCl buffer solution (50 mM Tris + 10 mM EDTA, pH 8.5) and 0.2 ml of 7.2 mM pyrogallol were added and reacted at 25 ° C. for 10 minutes, and then 0.1 N 1.0N HCl was added thereto. The reaction was stopped and the amount of oxidized pyrogallol in the reaction solution was measured at 420 nm. SOD-like activity is shown in the absorbance reduction rate of the test and control of the sample solution is shown in Table 2 below.

sample Sample concentration (µg / mL) SOD-like activity (%) CHW  10 3.0 ± 0.8 100 3.4 ± 1.3 700  7.7 ± 0.2 CHE 10 2.1 ± 0.5 100 6.3 ± 2.1 700 10.7 ± 0.6

The SOD-like activity of CH extract showed lower activity compared to the electron donating ability of the above experiment, but it showed a significant increase as the concentration increased, and the ethanol extract showed relatively higher activity than the hydrothermal extract.

Experimental Example 2. Anticancer effect measurement by MTT evaluation method

The assay of CH extracts against cancer cell lines was performed by MTT (3- (4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2H-tetrazolium bromide) test according to Carmichael's method. (Carmichael J, De Graff WG, et al., Cancer Res ., 47 (4) , pp936-942, 1987).

That is, 180 μl of cancer cell lines were divided into 96 well plates at 1 × 10 ⁴ cell / well, and 20 μl of samples were prepared by concentration, followed by incubation for 48 hours at 37 ° C. and 5% CO ₂ incubator. The same amount of distilled water was added and cultured under the same conditions. 20 μl of MTT solution prepared at a concentration of 5 mg / ml was added thereto, followed by incubation for 4 hours, and then culture medium was removed, and 150 μl of DMSO: EtOH (1: 1) was added to each well, followed by stirring for 30 minutes, followed by ELISA reader. By measuring the absorbance at 550nm to confirm the growth inhibitory effect of the cancer cell line are shown in Table 3 below.

CHW (μg / ml) CHE (μg / ml) 10 100 1000 10 100 1000 Skin Cancer Cell Line (G361) 15.4 ± 2.2 24.5 ± 7.8 75.2 ± 5.5 11.1 ± 3.1 19.6 ± 4.5 67.6 ± 6.8 Skin Cancer Cell Line (B16F0) 19.5 ± 6.2 17.4 ± 3.3 88.3 ± 4.1 14.5 ± 5.9 23.5 ± 3.9 89.9 ± 3.8 Breast Cancer Cell Line (MDA) 16.8 ± 5.6 29.0 ± 3.6 93.1 ± 1.8 15.9 ± 3.3 30.3 ± 9.1 86.5 ± 7.2

In all cancer cells, cell proliferation inhibition rate increased with increasing sample concentration. CH extract showed low proliferation inhibitory effect of less than 30% in both hot water extract and ethanol extract at 100㎍ / ㎖, but G361, B16F10, Inhibition of cell proliferation of hot water extract and ethanol extract of MDA showed over 70% growth inhibition at 1000 µg / ml. Especially, the inhibitory effect of B16F10 and MDA was 88.2% and 93.1%, respectively, at 1000㎍ / ㎖ for hot water extract, and 89.8% and 86.5% at 1000㎍ / mL for ethanol extract, respectively. Excellent cancer cell inhibitory activity was confirmed.

Experimental Example 3. Measurement of antimicrobial activity by the method of measuring growth zone

Staphylococcus aureus ( Staphylococcus aureus), Staphylococcus epidermidis ( Staphylococcus epidermidis) and oral bacteria Streptococcus mutans were cultured. All strains were incubated at 37 ° C. in a BOD incubator. Antibacterial activity was measured by a paper disc method. Incubation was performed at 35 ° C. for 18-24 hours to determine the diameter of the clear zone (mm) around the disc.

Test strain Concentration (mg / disc) 0.5 2.5 5 CHW CHE CHW CHE CHW CHE Staphylococcus aureus ^-b - 13.5 ^a 14.5 29.5 30 Staphylococcus epidermis - - 9 10.5 11 12.5 Streptococcus mutans - - - - 11 12.5 a: inhibitory capacity in diameter (mm), b: no action

As shown in Table 4, the antimicrobial effects of Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus mutans strains were 2.5 and 5 mg / disc, as shown in Table 4. Appeared very high. In particular, Staphylococcus aureus strains showed antimicrobial activity of hot water extract and ethanol extract of 13.5 and 14.5 mm at 2.5 mg / disc, respectively, and very high antimicrobial activity of 29.5 and 30.0 mm at 5 mg / disc. It was able to confirm the excellent antimicrobial effect of the CH extract of the present invention.

Experimental Example 4. Patch test

In order to determine the degree of skin irritation with respect to the CH extract according to the present invention, a skin patch test was carried out for Example 2. The subjects were selected to have no skin disease at the test site of 20 ~ 50 years old. Wipe the upper arm of the patch area with 70% ethanol and dry it. Then, apply petrolatum to the blank using a fin chamber and add 0.02 g of skin lotion of Example 2 to the upper arm of the human body for 24 hours, and remove the patch. The skin reaction was examined within 1 hour afterwards, and examined again the next day (after 48 hours). Skin response was determined by the test criteria of Table 5, the evaluation of the skin irritation probability was the average response rate calculated from the following equation 1, the skin patch test results for the cosmetic according to Example 2 in Table 6 Indicated.

Average response rate (%) = [Σ (Number of response subjects after 24 hours × score) + Σ (Number of response subjects after 48 hours × score)] × 100 / (Total number of tests × maximum score × number of subjects)

Skin reaction score Degree of reaction - 0 no response ± One Weak positive reaction (erythema) + 2 Positive reaction (erythema) ++ 3 Strong positive reaction (erythema, edema) +++ 4 Severe positive reactions (erythema, edema, blisters)

Test result of human body patch test Test substance Number of subjects responding after 24 hours (persons) Number of subjects responding after 48 hours (persons) Average response rate (%) 0 One 2 3 4 0 One 2 3 4 Example 2 15 0 0 0 0 15 0 0 0 0 0

As a result of the human patch test test, it was confirmed that the composition containing Example 2 is a substance that is safe for the human body within the non-irritating range.

As described above, the cosmetic composition containing the mixed herbal extract of the present invention shows an antioxidant effect in the electron donating ability and SOD-like activity test, growth inhibition effect on cancer cell lines and growth against skin flora and oral bacterial strains It can be usefully used as a cosmetic composition for antioxidant, anticancer and antimicrobial bar showing inhibitory activity.

Claims (5)

Staphylococcus aureus , Staphylococcus epidermidis And Streptococcus mutans Gold silver flower, red peony, raw yellow sulfur, cheonggung, donkey, rhubarb, sanchija, yeonkyo and licorice which shows antimicrobial effect on strain 1 ~ 10: 1 ~ 7: 1 ~ 7: 1 ~ 7: 1 ~ 4: 1 ~ 4: 1 Cosmetic composition containing the water or ethanol extract of the mixed herbal which is a dry weight ratio (w / w) of 1-4: 1-4. delete delete delete According to claim 1, wherein the extract is a cosmetic composition, characterized in that contained in 0.01 to 5% by weight relative to the total weight of the composition.