KR20070041927A - Composition comprising an extract of persicae flos having anti-oxidizing, anti-cancer, whitening activities - Google Patents

Abstract

Peachus ( Prunus) persica (L.) BATSCH) flower, that is, a composition containing the extract of Persicae flos as an active ingredient and a method for preparing the same, specifically, the extract of the present invention has excellent electron donating ability, SOD-like activity, large Xanthine oxidase inhibitory activity, tyrosinase inhibitory activity, showing a proliferation inhibitory effect on various cancer cell lines can be usefully used as an antioxidant, anti-cancer and whitening composition.

Painting, antioxidant, anti-cancer, whitening, cosmetics, health food

Description

Composition comprising an extract of Persicae flos having anti-oxidizing, anti-cancer, whitening activities}

1 is a diagram showing the proliferation inhibition rate of Dohwa extract for lung cancer cell line A549,

Figure 2 is a diagram showing the growth inhibition rate of the hwahwa extract for colon cancer cell line HT-29,

Figure 3 is a diagram showing the growth inhibition rate of the Dohwa extract for hepatic cancer cell line HepG2,

Figure 4 is a diagram showing the growth inhibition rate of the Dohwa extract for skin cancer cell line G361,

Figure 5 is a diagram showing the growth inhibition rate of the Dohwa extract for mouse skin cancer cell line B16F10.

The present invention relates to a composition containing a hwahwa extract having antioxidant, anticancer and whitening effects.

Antioxidant activity refers to an activity that prevents the production of free radicals in vivo and prevents oxidative phenomena that cause irreparable damage to cells. Steady state oxygen (triplet oxygen) is a superoxide radical, hydroxyl radical, hydrogen peroxide (H ₂ O ₂ ) due to environmental and biochemical factors such as enzymes, reduction metabolism, chemicals, pollutants, photochemical reactions, etc. It is known to convert into reactive oxygen species (ROS) such as ROS, thereby oxidizing various cell components such as lipids, proteins, and DNA, causing inflammation or damaging various organs (Beckman JS, et al., Proc . Natl . Acad . Sci . USA , 87 , pp . 1620-1624, 1990; Sagar S, et al., Mol. Cell Biochem ., 111, pp . 103-108, 1992; Ames BN, et al., Proc. Natl . Acad, USA, 90, pp. 7915-7922, 1993). Examples include superoxide anions (O ₂ ), hydrogen peroxide, hydroxyl radicals, singlet oxygen ( ¹ O ₂ ), and alkoxyl radicals (RO) produced by lipid peroxidation. , Peroxyl radical (ROO ·) and nitrogen peroxide ion (ONOO-).

The action of these free radicals is the action of antioxidant enzymes such as superoxide dismutase (SOD), catalase, and peroxidase, which are the body's defense mechanisms, and antioxidants such as vitamins C, E, and glutathione. However, if this biodefense is abnormal or exposed to excessive free radicals, this balance is broken and the free radicals irreversibly destroy substrates, proteins and DNA. It has been reported to cause a variety of diseases, such as aging, cancer, multiple atherosclerosis, arthritis and parkinson's disease (Griffiths HR, et al., FEBS) . Lett ., 388, pp. 161-164, 1996; Squadrito GL, et al., Free Radic . Biol . Med ., 25, pp. 392-493, 1998; Choi JS, Phytochem . Res ., 16 , pp. 232-235, 2002; Dreher D, et al., Eur . J. Cancer , 32A (1), pp. 30-38, 1996; Sohal RS, et al., Free Radic . Biol . Med ., 33 (1), pp. 37-44, 2002).

In order to prevent diseases caused by oxidative stress and prevent oxidative deterioration of foods, many studies are being conducted to isolate, identify and develop powerful and harmless natural antioxidants from edible or medicinal plants. Plant-derived natural compounds also retard the oxidation process as free radicals and scavengers or reducing agents of reactive oxygen species (Pratt, DE, American Chemical Society , pp. 54-71, 1994: Larson, RA, Phytochem ., 27 , pp. 969-978, 1988).

Synthetic antioxidants commonly used in cosmetics, food or pharmaceuticals include butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG, propyl galate) and tertiary Butyl hydroquinone (TBHQ, Tertiary butyl hydroquinone) and the like, but when administered to high concentrations in experimental animals are known to cause liver hypertrophy or carcinogenicity. In particular, butylated hydroxytoluene has been shown to increase microsomal enzyme activity in the liver of laboratory animals through several studies, and controversy has been raised about the safety of these phenolic synthetic antioxidants. Usage is legally regulated (Brannen AL, J. Amer . Oil Chem . Soc . , 52 , pp59-63, 1975; Ito N et al., J. Natl . Cancer Inst . , 70 , p343, 1983; Chan KM et al., J. Food. Sci., 58, pp1-4, 1993).

Natural products are mostly weak in inhibiting free radicals, but the safety of human skin is guaranteed. Therefore, active oxygen inhibitory effect and the development of materials that antioxidant effect is strong and safety is recognized also in the human skin is urgently required (Park SN, Jr. Soc. Cos . Sci. Korea, 23 (1), p75, 1997)

Human skin color is determined by the amount of melanin, carotene and hemoglobin, of which melanin is the most decisive factor. Melanin is synthesized in melanocytes, which are pigment cells in the basal layer of the epidermis, and transferred to surrounding keratinocytes to determine skin color. Tyrosinase (tyrosinase; monophenol, dihydroxy-L-phenylalanine: oxygen oxidoreductase, EC 1.14.18.1) is a copper-containing enzyme that uses a wide range of phenolic compounds as a substrate, and L-3,4- using tyrosine as a substrate. Melanine is produced by polymerization of each product through successive enzymatic oxidation and oxidation to produce dihydroxyphenylalanine (L-3,4-dihydroxyphenylalanine) and transfer it back to dopaquinone (L-DOPAquinone). melanin) is biosynthesized. Ultraviolet melanocyte mitosis causes melanocytes to be activated, and activated melanocytes stimulate tyrosinase synthesis to promote melanin production, and excess melanin is eventually transported and released out of the epidermis. It causes pigmentation such as freckles. Abnormally over-producing melanin produces blemishes and freckles, whereas less-producing melanin causes skin lesions such as alum. Pigmentation is caused by various stimuli such as ultraviolet rays and inflammation from the outside, and the degree of pigmentation is the amount of reaction of melanocytes present in the basement layer of the epidermis. Central to the pigmentation mechanism is tyrosinase.

As inhibitors of tyrosinase, cinnamic acid, benzoic acid, cysteine, chlorogenic acid, etc. are known, and melanostatin (hydroquinone), which is mainly used for blemish healing in women, Melanostatin, tropolone, kojic acid, arbutin, and the like have been used, but there are many problems such as safety and the effects are not clearly or clearly identified. Therefore, attempts have been made to use substances that have high tyrosinase inhibitory ability from natural products that humans have eaten safely for a long time.

In addition, cancer is one of the incurable diseases to be solved by humankind, and huge capital is invested in the development to cure it all over the world.In Korea, it is the number one disease death cause and more than 100,000 people per year. It is diagnosed, and about 60,000 or more die. Carcinogens, which cause cancer, are smoking, ultraviolet rays, chemicals, foods, and other environmental factors. However, various causes are not only difficult to develop a therapeutic agent, but also effective effects vary depending on the site of occurrence. The substances currently used as therapeutic agents are highly toxic and do not selectively remove cancer cells. Therefore, there is an urgent need for the development of low-toxic and effective anti-cancer drugs to prevent the development of cancer as well as its treatment after cancer. Do.

Drawing ( Persicae flos ) is a flower of peach tree and wild berry, and Prunus persica (L.) BATSCH is a deciduous arborescent of the Rosaceae plant native to China, Japan, and China. It is cultivated. Dohwa, a flower of peach tree, is bisexual, 2.5 ~ 3.5 ㎝ in diameter, with few peduncles, hairs next to calyx, usually pink, white, dark red, and other varieties, and is collected in the bloom in mid-April or early May. It is dried and used as herbal medicine. The taste is bitter, the property is flat, and it enters the heart and liver (Information and Shin Mingyo, Doha Hyangje Ambassador, Younglimsa, p633, 1998). Ingredients include kaempferol, coumarin, albamyricetin, and trifolin. In the case of flavonoid-derived camphorol, it has been reported to have lipid peroxidation and cholesterol inhibitory effects, but other researches as functional natural products have not been conducted through systematic studies on physiologically active functions.

However, none of this document discloses or teaches any composition for containing a extract that has antioxidant, anticancer and whitening activity.

      Accordingly, the present inventors have completed the present invention by confirming that the extract has excellent electron donating ability, SOD-like activity, xanthine oxidase inhibitory activity, proliferation inhibitory effect and various tyrosinase inhibitory activity against various cancer cell lines.

It is an object of the present invention to provide a cosmetic and health functional composition containing a hwahwa extract having excellent electron donating ability, SOD-like activity, xanthine oxidase inhibitory activity, proliferation inhibitory effect on various cancer cell lines and tyrosinase inhibitory activity.

In order to achieve the above object, the present invention provides an antioxidant, anticancer and whitening activity ( Persicae flos ) to provide a cosmetic composition containing the extract.

The doping extract includes water containing purified water, lower alcohol having 1 to 4 carbon atoms or propylene glycol, an extract available in at least one solvent selected from the group consisting of ethyl acetate, butylene glycol, ether, chloroform.

The cosmetic composition also includes formulations of flexible lotion (skin), nourishing lotion and nourishing cream.

In addition, the present invention is an antioxidant, anti-cancer and whitening activity ( Persicae flos ) Provides a dietary supplement comprising extracts and food acceptable food supplement additives.

Hereinafter, the present invention will be described in detail.

The cultivated extract of the present invention can be obtained as follows.

After collecting the drawing of the present invention to remove foreign matter, washing and drying, the C ₁ to C, such as water, ethanol and methanol in a volume of about 1 to 30 times, preferably about 1 to 15 times the weight of the sample ₄ is a polar solvent of the lower alcohol or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably water or 70% ethanol at about 10 to 100 ° C. for about 1 hour to 2 days Preferably, using extraction methods such as hot water extraction, reflux circulation extraction, dipping extraction or pressure extraction for 2 to 15 hours, preferably hot water reflux extraction and dipping extraction, centrifugation, vacuum filtration, concentration and lyophilization To obtain the extract of the present invention.

As a result of measuring the electron donating ability, SOD-like activity, xanthine oxidase inhibitory activity, proliferation inhibitory effect and tyrosinase inhibitory activity against various cancer cell lines, the antioxidant effect, anticancer effect and whitening effect were excellent. It can be usefully used as a cosmetic composition for antioxidant, anticancer and whitening.

In addition, Dohwa has been used for herbal medicine and food for a long time, the extracts of the present invention extracted from them also have no problems such as toxicity and side effects, and proved to be a non-irritating sample in the skin patch test, so it is safe to use even for long-term use. Can be.

The composition of the present invention comprises 0.001 to 10% by weight, preferably 0.001 to 5% by weight, most preferably 0.001 to 1% by weight, based on the total weight of the cosmetic composition.

The composition comprising the hwahwa extract of the present invention can be used in various ways, such as cosmetics, face wash and shampoo for the anti-aging, cancer prevention and whitening effect of the skin. Examples of the product to which the present composition may be added include various cosmetic compositions such as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, and moisturizing cream. , Hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. Derivatives thereof (palmitin ascorbic acid, ascorbic stearate, dipalmitin ascorbin, acetate dl-α-tocopherol, nicotinic acid dl-α-tocopherolvitamin E, DL-pantothenyl alcohol, D-panto Tenyl alcohol, pantothenyl ethyl ether and the like) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, pit spingosine, sphingolipid lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, lauric acid hexyl, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactic acid, cetyl lactate, octyl lactate, trit citrate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methyl steoxysiloxane copolymer and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE · POP (PolyoxyethylenePolyoxypropylene) Copolymer, POEPOP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, coloramine, carbon black, and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate and benzyl cinnamic acid. , Paramethoxy cinnamic acid 2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono 2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urocanine Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% compounding.

The cosmetic of the present invention may take the form of solutions, emulsions, viscous mixtures and the like.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the hwahwa extract as an active ingredient, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments and flavorings. It includes.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specific examples of the cosmetic of the present invention include a flexible lotion, nourishing lotion, nourishing cream, cleansing cream, face wash, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, pack, aftershave cream, sunburn prevention cream Suntan oil, body shampoo, hair shampoo, hair rinse, hand cream, foundation, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, there is provided a health functional food containing an antioxidant, anti-cancer and whitening activity obtained in the extraction process of the present invention as an active ingredient.

As defined herein, "health functional food" means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act No. 6767 of the Health Functional Food Act, and "functional" means It means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action on structure and function.

The dietary supplement for anti-aging, whitening, cancer disease prevention and improvement of the skin of the present invention comprises the extract in an amount of 0.01 to 95%, preferably 1 to 80% by weight based on the total weight of the composition.

In addition, it is possible to manufacture and process as a health functional food in the form of tablets, capsules, powders, granules, liquids, pills, etc. for the purpose of preventing aging, whitening, and preventing and improving skin diseases.

For example, the health functional food in the form of tablets may be prepared as it is or granularly mixed with an excipient, a binder, a disintegrating agent or other additives in a suitable manner, and then compressed into a glidant, etc. It is made by directly compressing the functional food as it is or by mixing it evenly with an excipient, binder, disintegrant or other suitable additives, or mixing the health functional food as it is or evenly adding the appropriate additive to the prepared granules, The excipient, binder or other suitable additives are added to the functional food, and the powder mixed evenly is wetted with a solvent, the wet powder is molded into a mold at low pressure, and then dried and prepared by a suitable method. In addition, the nutraceutical can be added to the health functional food in the form of tablets, if necessary, can be avoided with a suitable epidermis.

Among the health functional foods in the form of capsules, the hard capsules are usually prepared by mixing the capsules evenly with the health functional foods or excipients suitable for the health functional foods, granulated by a suitable method, or granulated with a suitable epidermal agent as it is or It is formulated by filling lightly and filled.In soft capsules, regular capsules are prepared by adding appropriate excipients for health functional foods or health functional foods with glycine or sorbitol, etc. It is molded into a shape and prepared, and a coloring preservative etc. can be added to the said capsule base as needed.

Circular functional foods are usually mixed with excipients, binders, disintegrants, etc., and then molded into a spherical form in a suitable manner, and the coating is carried out with white sugar or other suitable coating agent, or starch, talc or You can also be greeted with a suitable substance.

The health functional food in the form of granules is usually made by mixing the health functional food as it is or by adding excipients, binders, disintegrating agents, etc., evenly and then granulating it in a proper way to make the particles as even as possible. , Copulation agent, etc. For the health functional food in the form of granules, No. 12 (1680 μm), No. 14 (1410 μm) and No. 45 (350 μm) were used for the next particle size test. It should be less than 5.0% and pass through No.45 to less than 15.0% of the total amount.

The definitions of the excipients, binders, disintegrants, lubricants, copulation agents, flavoring agents, etc. of the present invention are those described in the literature known in the art and include those having the same or similar functions. , Korean College of Pharmacy, 5th Edition, p33-48, 1989).

Below, The invention is illustrated in detail by the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1. Preparation of Sample Extract

1-1. Drawing Hydrothermal  Preparation of Extract

Dohwa, used in the present invention, was collected in early April by collecting peach trees grown in Gyeongsan, Gyeongsangbuk-do, to remove foreign substances, washed and then used in the shade. Distilled water was added at a ratio of 1: 10 (w / v) at 25 g of dry water for 3 hours at reflux for 3 hours at 85 ° C, followed by centrifugation at 4 ° C and 8,000 × g for 30 minutes (Centrifuge; CR21, Hitachi , Japan), and the supernatant obtained by filtration with Wattman No. 2 (Advantec TOYO, Japan) and concentrated under reduced pressure with a rotary vacuum evaporator (NN SERIES, Eyela Tokyo, Japan) at 30 to 60 ℃. It was lyophilized to give 3.97 g of powder, which was used as various analytical samples (hereinafter referred to as PFW).

1-2. Preparation of Doped 70% Ethanol Extract

70 g of ethanol is added to 75 g of the dry gas in a ratio of 1: 10 (w / v), immersed and extracted for 24 hours at 25 to 30 ° C., and then filtered and a rotary vacuum evaporator at 30 to 60 ° C .; NN SERIES, Eyela Tokyo, Japan), concentrated under reduced pressure and lyophilized to obtain 8.47 g of a powder, which was used as a sample for analysis (hereinafter referred to as PFE).

Example  2. Preparation of Skin Lotion

Skin lotion was prepared using the PFW of Example 1 above.

number Raw material Composition (% by weight) One PFW 1.0 2 Butylene glycol 5.0 3 glycerin 3.0 4 Carboxy Vinyl Polymer 0.06 5 Disodium Ethylenediaminetetraacetic Acid 0.01 6 ethanol 7.0 7 Sorbitan monostearate 0.8 8 Triethanolamine 0.2 9 Hydrogenated Castor Oil 0.4 10 Phenyltrimethicone 0.5 11 Paraoxybenzoic acid propyl 0.2 12 Methyl paraoxybenzoate 0.2 13 Spices 0.1 14 Purified water Remaining amount system 100.0

Experimental Example  1. Dohwa extract Antioxidant power  Measure

1-1. Electron donating ability  (Electron donating ability: EDA) measurement

Determination of the electron donating ability of the extract obtained in Example 1 according to the method of Blois (Blois MS et al., Nature , 181, pp1199-1200, 1958) DPPH (1,1-diphenyl-2-picryl hydrazyl) It was measured by the hydrogen donating effect on. That is, 1 ml of 0.2 mM DPPH solution (dissolved in 99% Ethanol) was added to 2 ml of a sample at a constant concentration, vortexed and mixed for about 10 seconds, and reacted at 37 ° C. for 30 minutes. The reaction solution was measured for absorbance at 517 nm using an Absorption Spectrophotometer (Hitachi UV-2001, Japan). The electron donating ability of DPH extract of DPPH was calculated by substituting Equation 1 below.

As shown in Table 2, when the concentration of the extract of Example 1 was 0.01 to 1 mg / ml, the electron donating ability was 2.2 to 60.9 and PFE was 5.0 to 93.2, respectively. In both types of extracts, the higher the concentration of the sample, the higher the electron donating ability of DPPH. The PFE showed higher electron donating ability than PFW at all concentrations. In the case of PFW, about 60% at 1 mg / ml and PFE at 1 mg / ml showed excellent electron donating ability of about 90% or more.

   1- (absorbance of sample solution added / non-added sphere) × 100

Example 1 Treatment Concentration of Extract (mg / ml) Electron donating ability (%) PFW PFE 0.01 2.2 ± 2.0 5.0 ± 2.5 0.05 26.6 ± 0.8 33.6 ± 0.6 0.1 46.5 ± 0.9 69.4 ± 0.3 0.5 61.0 ± 1.2 92.4 ± 0.0 One 60.9 ± 0.3 93.2 ± 0.0

1-2. SOD Pseudo-activity  ( Superoxide dismutase  like activity: SODA) measurement

SOD-like activity of the cultivated extract obtained in Example 1 was catalyzed by the conversion of hydrogen peroxide according to the method of Marklund et al., Marklund S et al., Eur . J. Biochem . , 47 , pp468-474, 1975. The production amount of pyrogallol was measured and shown. In other words, 2.6 ml of Tris-HCl buffer (50 mM Tris [hydroxymethyl] amino methane, 10 mM EDTA, pH 8.5) and 0.2 ml of 7.2 mM pyrogallol were added to 0.2 ml of sample at a constant concentration of 25 ° C. After the reaction at 10 minutes, 1 ml of 1 N HCl was added to stop the reaction. The amount of oxidized pyrogallol in the reaction solution was measured for absorbance at 420 nm using a UV / VIS spectrophotometer (AAS, Hitachi Z-6000, Japan), and the SOD-like activity of the doped extract of Example 1 was substituted in Equation 1 above. Calculated by As shown in Table 3, PFW showed similar activity of 30.7% at 1 mg / ml, and PFE showed 30.7% of similar activity at 1 mg / ml.

Example 1 Treatment Concentration of Extract (mg / ml) SOD-like activity (%) PFW PFE 0.01 0.6 ± 1.5 0.7 ± 1.0 0.05 4.0 ± 0.7 3.9 ± 0.4 0.1 3.5 ± 0.4 3.4 ± 0.2 0.5 16.9 ± 1.7 12.8 ± 0.0 1.0 30.7 ± 3.0 31.5 ± 3.0

1-3. Xanthine oxidase ( Xanthine oxidase ) Inhibitory activity measurement

100 μl of sample and 600 μl of 0.1 M phosphate buffer pH 7.5 were added to 200 μl of 2 mM xanthine substrate solution, followed by xanthine oxidase (0.2 U / ml, SIGMA Co., Ltd.). , USA) 100 μl was added. The xanthine oxidase activity was measured for absorbance at 295 nm for uric acid (uric acid) produced in response to xanthine at 37 ° C. for 15 minutes.

As shown in Table 4, the low inhibitory activity (EC ₅₀ <3.1 mg / ml) of 0.5 mg / ml for PFW was lower than 10%, while the inhibitory activity of 31.1% at 0.5 mg / ml for PFE (EC _50). <0.8 mg / ml).

Example 1 Treatment Concentration of Extract (mg / ml) Xanthine oxidase inhibitory activity (%) PFW PFE 0.01 1.8 ± 2.0 5.5 ± 2.8 0.05 0.9 ± 1.3 2.9 ± 3.7 0.1 0.2 ± 0.2 11.5 ± 1.8 0.25 2.3 ± 4.4 16.1 ± 3.0 0.5 7.9 ± 1.7 31.1 ± 2.2

Experimental Example  2. Dohwa extract Tyrosinase  Inhibitory activity measurement

To 500 µl of 67 mM sodium phosphate buffer (pH 6.8), 200 µl of 10 mM L-DOPA substrate solution and 0.1 ml of the sample solution of Example 1 were mixed with mushroom tyrosinase (110 U / ml, mushroom tyrosinase, Sigma). USA) 200 μl was added and reacted at 25 ° C. for 2 minutes, and then the amount of dopachrome produced in the reaction solution was measured at 475 nm. Tyrosinase inhibitory activity of the extract was calculated by substituting Equation 1 above.

As shown in Table 5, PFW showed a low inhibitory activity (IC ₅₀ <1.8 mg / ml) of less than 28% at 1 mg / ml, whereas PFE showed a 40.5% inhibitory activity (IC ₅₀ at 1 mg / ml). <0.8 mg / ml).

Example 1 Treatment Concentration of Extract (mg / ml) Tyrosinase Inhibitory Activity (%) PFW PFE 0.01 0.6 ± 3.1 29.4 ± 0.4 0.05 0.6 ± 0.8 29.7 ± 0.0 0.1 0.7 ± 0.9 30.0 ± 0.6 0.5 14.9 ± 1.0 35.3 ± 0.6 One 28.0 ± 0.6 40.5 ± 0.2

Experimental Example  3. Anticancer Activity of Dohwa Extracts

Proliferation inhibitory effect of hwahwa extract on cancer cell lines was measured by MTT evaluation method. Cancer cell lines used in the experiment were purchased from Korean Cell Line Bank (KCLB), skin cancer cells B16F10 and G361, liver cancer cells HepG2, colon cancer cells HT-29 and lung cancer cells A549. Each cell was cultured using RPMI 1640 medium (GIBCO, Scotland) with 10% fetal bovine serum (FBS) and 1% penicillin / streptomycin (100 U / mL). Subcultured by adapting to a 5% CO 2 incubator. Each cancer cell line of logarithmic growth phase was filled with 180 μl of medium per well in a 96 well plate, and 6 ~ 8 × 10 ³ cells were dispensed. Incubated for 24 hours in a 37 ℃, 5% CO ₂ incubator, the control group was incubated under the same conditions by adding the same amount of distilled water. 20 μl of the MTT solution prepared at a concentration of 5 mg / ml was added thereto, followed by incubation for 4 hours, and then the culture medium was removed, and dimethyl sulfoxide (DMSO): ethanol = 1: 1 150 μl was added to each well for 30 minutes at room temperature. After the reaction, the absorbance was measured at 550 nm using an ELISA reader (Emax, Moleular Devices, Sunnyvale, USA). Anticancer activity of the Dohwa extract was calculated by substituting Equation 1, shown in Table 6, Table 7 and Figures 1 to 5.

PFW inhibition of cancer cell proliferation (%) Extract Treatment Concentration (mg / ml) 0.05 0.1 0.5 One Lung Cancer (A549) 20.7 ± 3.9 20.1 ± 4.2 34.3 ± 2.1 33.0 ± 3.6 Colon Cancer (HT-29) 12.2 ± 2.0 12.0 ± 3.5 8.7 ± 0.1 12.2 ± 1.9 Liver Cancer (HepG2) 45.8 ± 8.9 50.0 ± 13.5 82.6 ± 0.7 79.7 ± 1.0 Skin Cancer (G361) 11.7 ± 7.4 31.6 ± 3.5 66.1 ± 9.0 79.5 ± 1.5 Skin Cancer (B16F10) 4.0 ± 1.2 17.9 ± 7.5 84.9 ± 5.0 90.0 ± 1.2

PFE inhibition of cancer cell proliferation (%) Extract Treatment Concentration (mg / ml) 0.05 0.1 0.5 One Lung Cancer (A549) 16.0 ± 1.7 19.0 ± 5.0 30.8 ± 3.6 32.3 ± 2.4 Colon Cancer (HT-29) 5.4 ± 1.0 13.5 ± 4.5 20.5 ± 1.4 22.7 ± 4.1 Liver Cancer (HepG2) 20.6 ± 9.1 24.8 ± 10.2 34.9 ± 5.8 38.7 ± 4.7 Skin Cancer (G361) 11.7 ± 2.3 15.4 ± 4.3 19.9 ± 9.4 26.9 ± 12.8 Skin Cancer (B16F10) 17.4 ± 3.1 9.1 ± 4.0 30.0 ± 4.7 47.4 ± 13.0

As shown in Table 6 and Table 7, the inhibition rate of cancer cell proliferation also increased as the concentration of the sample in both the extracts, especially in the case of PFW, hepatic cancer cells (Hep G2) at a concentration of 0.5 ~ 1 mg / ㎖ ), Skin cancer cells (G361), skin cancer cells (B16F10) showed an excellent cancer cell growth inhibitory ability of 66.1 ~ 90.0% or more (see FIGS. 1 to 5).

Experimental Example  4. human body Patch test (Patch test)

In order to determine the degree of skin irritation with respect to the composition according to the present invention, a skin patch test was carried out for Example 2. The subjects were selected to have no skin disease at the test site of 20 ~ 50 years old. After wiping the upper arm area with 70% ethanol and drying it, petroleum jelly was added to the human upper arm part for 24 hours by adding petroleum jelly with a blank and 0.02 g of skin lotion of Example 2 using a fin chamber. The skin reaction was examined within 1 hour and retested the next day (after 48 hours). The skin response was determined by the test criteria of Table 8 below, and the evaluation of skin irritation probability was performed using the average response rate calculated from Equation 2 below. Indicated.

Average response rate (%) = [Σ (Number of response subjects after 24 hours × score) + Σ (Number of response subjects after 48 hours × score)] × 100 / (Total number of tests × maximum score × total number of subjects)

Skin reaction score - 0 no response ± One Weak positive reaction (erythema) + 2 Positive reaction (erythema) ++ 3 Strong positive reactions (erythema, edema) +++ 4 Severe positive reactions (erythema, edema, blisters)

Test substance Number of subjects responding after 24 hours (persons) Number of subjects responding after 48 hours (persons) Average response rate (%) 0 One 2 3 4 0 One 2 3 4 Example 2 15 0 0 0 0 15 0 0 0 0 0

As a result of the human patch test test, it was confirmed that the composition containing Example 2 is a substance that is safe for the human body within the non-irritating range.

Hereinafter, a softening lotion (skin), a lotion, an essence, a face wash (cleansing foam) and a shampoo are illustrated as examples of the formulation of the present invention, but the formulation including the cosmetic composition of the present invention is not limited thereto.

Formulation example  1. Flexible lotion ( skin )

Butylene glycol, glycerin, polyoxyethylene (60) hardened castor oil, betaine, citric acid, sodium citrate, and preservatives were added to the purified water, followed by stirring and dissolving. PFW was added thereto, stirred well, and aged to prepare a PFW-containing flexible lotion. Table 10 shows the content of each component.

Raw material Formulation Example 1 Content (w / w%) PFW 50.0 Butylene Glycol 7.0 glycerin 5.0 Polyoxyethylene (60) hardening castor oil 0.2 ethanol 5.0 Betaine 2.0 Citric acid 0.02 Sodium citrate 0.06 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  2. Lotion ( emulsion )

PFW, butylene glycol, glycerin, carboxyvinyl polymer, arginine, preservative and purified water obtained in the above examples were heated with stirring at 70 to 75 ° C. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate, and stearyl glycerate mixtures heated and stirred at 75 to 80 ° C. were added thereto to emulsify, When the mixture was cooled to about 45 ° C. while stirring, the fragrance was added thereto, stirred, cooled to 30 ° C., and aged to prepare a PFW-containing lotion. Table 11 shows the content of each component.

Raw material Formulation Example 2 Content (w / w%) PFW 40.0 Butylene glycol 8.0 glycerin 5.0 Squalane 10.0 Butylene Glycol Dicaprylate / Dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.0 Glyceryl Stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxy Vinyl Polymer 0.1 Arginine 0.1 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  3. Essence

Cytocyterol, polyglyceryl 2-oleate, ceramide, ceteareth-4 and cholesterol are mixed by stirring, followed by emulsification by adding a mixed solution of PFW, dicetyl phosphate, concentrated glycerin and purified water, and then stirring at 45 ° C. After cooling, the fragrance was added and stirred, and the mixture was cooled to 30 ° C and aged. Carboxyvinyl polymer, xanthan gum and preservatives were added thereto, stabilized, and aged to prepare PFW-containing essences. Table 12 shows the content of each component.

Raw material Formulation Example 3 Content (w / w%) PFW 10.0 Cytostolol 1.70 Polyglyceryl 2-oleate 1.50 Ceramide 0.7 Ceteares-4 1.2 cholesterol 1.5 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Carboxy Vinyl Polymer 0.2 Xanthan Gum 0.2 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  4. Cleanser (Cleansing Foam)

Sodium N-acyl glutamate, glycerin, PEG-400, and propylene glycol were added and mixed with purified water, and then PFW was added in small portions, and dissolved by heating at 80 ° C. while adding EDTA-4Na. POE (15) oleyl alcohol ether, laurin derivative and methyl paraben mixed solution heated at 80 ° C. were added thereto, stirred, and the mixture was gradually cooled to prepare a PFW-containing cleanser. Table 13 shows the content of each component.

Raw material Formulation Example 4 Content (w / w%) PFW 20.0 N-acyl glutamate 20.0 glycerin 10.0 PEG-400 15.0 Propylene glycol 10.0 POE (15) oleyl alcohol ether 3.0 Lauric derivatives 2.0 Methyl paraben 0.2 EDTA-4Na 0.03 Spices 0.2 Purified water Remaining amount

Formulation example  5. Shampoo

Glycerine and EDTA-4Na were added to purified water, and the resulting mixture was heated and dissolved at 80 DEG C, followed by stirring by adding TEA lauryl sulfate, sodium lauryl ether sulfate, lauryl amidopropyl betaine and lauryl acid diethanol amide. Citric acid was added thereto and neutralized at 50 ° C., and then PFW and zinc pyrithione were added and stirred at 45 ° C. to prepare a PFW-containing shampoo. Table 14 shows the content of each component.

Raw material Formulation Example 5 Content (w / w%) PFW 20.0 TEA Lauryl Sulfate 20.0 Sodium Lauryl Ether Sulfate 30.0 Lauryl Amidopropyl Betaine 2.0 Lauryl acid diethanol amide 3.0 glycerin 3.0 EDTA-2Na 0.05 Methyl paraben 0.2 Citric acid 0.03 Zinc pyrithione 0.02 Spices 0.2 Purified water Remaining amount

Dohwa extract of the present invention can be administered in the following formulation, the formulation examples below are merely to illustrate the invention, whereby the content of the present invention is not limited.

Formulation example  1. Manufacture of healthy food

PFW of Example 1 ... 1000 mg

Vitamin Blend ...............

Vitamin A Acetate ......... 70 μg

Vitamin E ............ 1.0 mg

Vitamin B1 ........................ 0.13 mg

Vitamin B2 ........................ 0.15 mg

Vitamin B6 ............... 0.5 mg

Vitamin B12 ............... 0.2 μg

Vitamin C ............ 10 mg

Biotin .............................. 10 ㎍

Nicotinamide ......... 1.7 mg

Folic acid ......................... 50 μg

Calcium Pantothenate ......... 0.5 mg

Inorganic mixtures ...............

Ferrous Sulfate ............... 1.75 mg

Zinc Oxide ............... 0.82 mg

Magnesium Carbonate ............... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dibasic calcium phosphate ............... 55 mg

Potassium Citrate ............... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride ........................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation example  2. Manufacture of health drinks

PFW of Example 1 ... 1000 mg

Citric Acid .................................... 1000 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ........................... 2 g

Taurine ......................................... 1 g

Purified water is added to the whole ..... 900 ㎖

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

As described above, the cultivated extract of the present invention has excellent electron donating ability, SOD-like activity, xanthine oxidase inhibitory activity, tyrosinase inhibitory activity, anti-proliferative effect on various cancer cell lines, as an antioxidant, anti-cancer and whitening composition. It can be usefully used.

Claims (7)

With antioxidant, anticancer and whitening activity ( Persicae flos ) Cosmetic composition containing the extract. The cosmetic composition of claim 1, wherein the extract is extracted with one or more solvents selected from the group consisting of water including purified water, lower alcohols having 1 to 4 carbon atoms or propylene glycol, ethyl acetate, butylene glycol, ether, and chloroform. According to claim 1, wherein the cosmetic extract, characterized in that 0.001 to 10% by weight of the extract. According to claim 1, wherein the cosmetic cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Cosmetic composition, characterized in that the formulation of any one selected from the group consisting of a pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. With antioxidant, anticancer and whitening activity ( Persicae flos ) A dietary supplement containing extracts and food acceptable food supplement additives. The health functional food according to claim 5, wherein the functionalized extract is contained in an amount of 0.01 to 95% by weight. A dietary supplement according to claim 5 which is a powder, granule, tablet, capsule or beverage.

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