KR100780893B1 - A composition comprising a fruit extract of elaeagnus multiflora showing anti-oxidant, anti-inflammatory activity and whitening effect - Google Patents

Abstract

A composition comprising an extract of Elaeagnus multiflora fruit is provided to inhibit DPPH free radical excellently and secure xanthin oxidase inhibitory capability, platelet coagulation inhibitory activity, nitrite formation inhibitory activity and tyrosinase inhibitory activity, thereby being usefully used for preventing and treating skin diseases and inflammatory diseases caused by oxidative stress. A pharmaceutical or cosmetic composition for preventing and improving skin diseases related to inflammation comprises a fruit extract of Elaeagnus multiflora prepared by using a solvent such as petroleum ether, chloroform or ethylacetate as an effective ingredient.

Description

A composition comprising a fruit extract of Elaeagnus multiflora showing anti-oxidant, anti-inflammatory activity and whitening effect}

1 is a diagram showing the DPPH free radical scavenging effect of the fruit extract of the garden barley, petroleum ether extract group (Petroleum ether extract, ▲), chloroform extract group (■), ethyl acetate extract group (ethyl acetate extract, ● ), Butanol extract group (butanol extract, △), water extract group (water extract, □), ascorbic acid group (ascorbic acid, ○) showing the DPPH free radical scavenging effect,

Figure 2 is a diagram showing the reducing power of the fruit extract of the garden barley, petroleum ether extract group (Petroleum ether extract, ▲), chloroform extract group (chloroform extract, ■), ethyl acetate extract group (ethyl acetate extract, ●), butanol extract group (butanol extract, △), water extract group (water extract, □), ascorbic acid group (ascorbic acid, ○) showing the reducing power,

3 is a diagram showing the xanthine oxidase inhibitory effect of the fruit extract of garden barley, petroleum ether extract group (Petroleum ether extract, ▲), chloroform extract group (chloroform extract, ■), ethyl acetate extract group (ethyl acetate extract, ● ), Butanol extract group (butanol extract, △), water extract group (water extract, □), ascorbic acid group (ascorbic acid, ○) showing the inhibitory effect of xanthine oxidase,

Figure 4 is a diagram showing the anti-platelet aggregation inhibitory effect of the fruit of the barley tree fruit extract,

Figure 5 is a view showing the nitrite production inhibitory effect of the fruit extract of the garden barley.

The present invention is a skin external pharmaceutical composition for the prevention and treatment of skin diseases and inflammatory diseases due to the oxidative stress containing the extract of garden barley fruit having antioxidant, anti-inflammatory and whitening activity and prevention of skin diseases and inflammatory diseases due to oxidative stress And to a cosmetic composition for improvement.

Antioxidation refers to the prevention of various oxidations in the body. Oxidation refers to a phenomenon in which various kinds of unsaturated fatty acids present in the body are oxidized by oxygen to produce a substance called lipid peroxide, which increases aging. (Superoxide), NO (nitric oxide ), NO 2 (nitrogen dioxide), OH (hydroxyl), ROO (proxyl), RO (alkoxyl), HO 2 (- O 2 occurring in human disease, and aging the body metabolic processes oxidative reactions such as hydroperoxyl radicals, and in vivo, as a means of defending the body from free radicals such as harmful radicals, superoxide dismutase (SOD), catalase (CAT) and glutathione Antioxidant enzymes such as peroxidase (GSHpx; glutathione peroxidase) and antioxidants such as vitamin E and glutathione are present to protect the living body from oxidative damage by removing free radicals (Tiidus PM. Et al, Sports Med , 20 , pp 12). -13, 1995). However, when these antioxidant defenses are too low to control the production of free radicals, oxidative stress and damage of tissues are promoted. Excess free radicals and lipid peroxides produced in the body are injured by oxidative stress such as protein oxidation and DNA damage. (Kumar CT, et al, Mol . Cell Biochem ., 111 , pp 109-115, 1992). Therefore, many synthetic or natural antioxidants have been developed to reduce this oxidative stress, but synthetic antioxidants have safety problems (Branen, AL, J. Am. Oil. Chem . Soc ., 55 , 123-126). , 1975; Ito, N., et al, J. Natl . Cancer Inst., 70 , pp343-349, 1983), and recently, to develop natural antioxidants that are safer and more effective in various herbal and edible plant extracts. (Lim, DK, et al, Korean J. Food Sci . Technol . , 28 , pp83-89, 1996; Kim, HK, et al, Korean J. Food Sci . Technol . , 27 , pp80- 85, 1995; Cha, JY, J. Korean Soc , Food Nutr . , 28 , pp1131-1136, 1999; Kim, MH, et al, Korean J. Food Sci . Technol ., 31 , pp273-279, 1999).

Antioxidants present in plant foods are typical of carotenoids, flavonoids, and polyphenols, along with vitamins A, C, and E.

Various biochemical phenomena are involved as a cause of inflammation in living bodies. Macrophage is a multifunctional cell that plays an important role in the inflammatory response by producing various cytokines and nitric oxides (NO) in oxidative stress situations. In particular, iNOS expressed by stimulation such as lipopolysaccharide (LPS), cytokine, TNF-α in macrophages produces large amounts of nitric oxide (NO) for a long time. This oxidative stress is known to promote NFκB activity, a transcription factor of the inflammatory response. NFκB, composed of p50 and p65 heterodimers, is known to promote gene expression that activates neutrophils such as iNOS and COX-2 after activating and moving to the nucleus. It is known to inhibit (Baeuerle P. et al., Annu . Rev. Immunol. , 12 , pp 141-179, 1994; Allenm R. et al, Free Radic . Biol . Med . , 28 , pp463-499, 2000) . Recent studies show that nitrogen monoxide (NO) is a free radical produced from arginine by three major NOS isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS). to be. nNOS and eNOS are controlled by Ca ^{2 +} / calmodulin (calmodulin), but, iNOS is regulated at the transcriptional level by inflammatory stimuli such as IL (interleukin), IFN (interferon), LPS. Nitric oxide (NO), produced in small quantities by nNOS or eNOS, is responsible for normal physiological functions such as vasodilation, neurotransmission, and cellular destruction of pathogens, but nitric oxide (NO), which is overproduced by iNOS in macrophages, is superficial. Reacts with superoxide to form peroxynitrite, which acts as a powerful oxidant, damaging cells and activating NFκB in macrophages activated by inflammatory stimuli, resulting in inflammation, cancer, and atherosclerosis. It is known to relate to chronic diseases (Lawrence T. et al., Nat Med . , 7 , pp1291-1297, 2001; Riehemann K. et al., FEBS Lett . , 442 , pp89-94, 1999; Kang JL. et al, Mol Cell Biochem, 215 , pp1-9, 2000;..... El-Mahmoudy A. et al, International Immunopharmacology, 2, pp1603-1611, 2002).

Nitric oxide (NO) is known to be produced by iNOS in various pathophysiological processes, including inflammation and cancer. NOS is an important enzyme that regulates inflammation, vasodilation, neurotransmission, tumor cell and body homeostasis, and produces nitric oxide (NO) from L-arginine, which is monitized by iNOS in macrophages in inflammatory conditions. It is known to produce large amounts of nitrogen (NO) and cause various chronic diseases (Kim KM. Et al., Free Radic . Biol . Med . , 30 (7) , pp747-756, 2001; Stamler J S. et al. , Science , 258 , pp 1898-1902, 1992). Expression of iNOS is induced by NFκB activity, which is an important mechanism by which LPS or cytokine is overproduced in macrophages. Nitric oxide (NO) production in macrophages is directly related to iNOS expression, and LPS is a Gram-negative bacterial cell wall material that stimulates immune cells and synergistically increases NO production. Let's do it.

NFκB is a transcription factor involved in various stages such as cytokine response, inflammation, and cell growth regulation. Recent studies strongly suggest that inducible NFκB is involved in the development of atherosclerosis. In many cells, NFκB is a redox-sensitive transcription factor that is involved in various signal transduction from the cytoplasm to the nucleus. It is found in the cytoplasm as a trimer of p50, p65 and IκB subunits, and when IκB is degraded by oxidative stress, the p50 / p65 heterodimer is transferred into the nucleus, resulting in continuous DNA binding. (Gius D. et al., Toxicol . Lett . , 106 , pp93-106, 1999).

Human skin color is determined by the amount of melanin, carotene and hemoglobin, of which melanin is the most decisive factor. Melanin is synthesized in melanocytes (melanocytes), which are pigment cells in the basal layer of the skin (epidermis), and are transferred to peripheral keratinocytes (Keratinocytes) to represent human skin color. An abnormally low production of melanin causes skin lesions such as vitiligo. Overproduction, on the other hand, forms one of the main concerns of middle-aged women, blemishes and freckles, and is also closely related to skin cancer (Melanoma).

The whitening action is described in more detail as follows. Melanin is produced by tyrosine (Tyrosine) through the enzyme and non-enzymatic oxidation reaction in the cell called melanocytes in the basal layer of the skin epidermis, and is transferred to the keratinocytes constituting the epidermis. Important enzymes involved in melanin biosynthesis include tyrosinase, tyrosinase-related protein 1 and dopachrome tautomerase. Two enzymes besides tyrosinase have been recently developed and recently attracted attention, but the enzyme that plays a decisive role in the synthesis of melanin is tyrosinase. The first step in biosynthesis of melanin is caused by tyrosinase. Tyrosinase causes tyrosine to be dopa (Dopa), which makes it dopaquinone (Dopa-quinone). The next steps of these two reactions are known to be possible by non-enzymatic reactions, and tyrosinase alone is known to produce melanin.

Recently, a new theory of melanin formation following the expression of signal transduction agents in skin cells has been proposed. That is, when the skin is exposed to ultraviolet light, interlukin-alpha, which causes inflammation, is expressed from keratinocytes in the epidermis, and this interleukin-alpha expresses keratinocyte endothelin. To promote. This series of processes increases the formation of melanocytes and activates melanocytes. In the melanoma in the melanocytes thus formed, melanin is formed by tyrosine tyrosine.

Prior arts include ascorbic acid (Japanese Patent Laid-Open Publication No. 4-9320), hydroquinone (Japanese Patent Laid-Open Publication No. 6-192062), kojic acid (Japanese Patent Laid-Open Publication No. 56-7710), and arbutin (Japanese Patent Laid-Open Publication No. 4). -9315) and some of the compounds have been disclosed as a tyrosinase inhibitory activity has been used as a raw material for the whitening cosmetics, but the stability in the prescription system is degraded due to the degradation of pigmentation, off-flavor, efficacy effects at the biological level and safety The use is limited due to problems. And the inhibitory effect of tyrosinase has been demonstrated, but the effect is low in experiments similar to the actual biologic level. Therefore, the inhibitory effect of the melanoma cell culture similar to the biological level is required. In addition, hydroquinone is regulated as a carcinogenic substance and is prohibited from use. Kojic acid and ascorbic acid are very unstable substances and browning occurs when a small amount of cosmetics are stored for several weeks at room temperature.

In order to solve the problems of existing raw materials with whitening effect by suppressing melanin production for a long time, various kinds of abnormal melanin production can be suppressed from high-efficiency plant extracts such as herbal medicines, vegetables, fruits, and flowers. Efforts have been made to find extracts that are effective, and plant natural products are not only excellent in safety but also have various beneficial ingredients, making them a good target for whitening substances.

Elaeagnaceae Genus Elaeagnus plants range from northern Asia to the Himalayas and Europe (Ahmadiani et al., Journal of Ethnopharmacology , 72, pp 287-292, 2000). Seeds of many plants of Elaeagnus contain vitamins, minerals, flavonoids and many other bioactive substances. And unlike other fruits, the fruit contains many essential fatty acids and is grown as a food that can reduce the incidence of cancer by stopping or reducing cancer growth (Matthews. V, Royal Horticultural Society , 1 , ISBN) 1352-4186, 1994).

Elaeagnus multiflora is a shrub that grows up to 2-3m and blooms from April to May, and matures about 2.5cm of edible fruit that is edible in June. In oriental medicine, this fruit is called Mokban-ha. It is a native species of China and Japan and has been cultivated for centuries as an ornamental and fruit tree. Also, in China, fruit, leaves, and roots are used for the treatment of colds, diarrhea, skin diseases, severe pain, and cancer (Duke et al., Reference Publications , Inc. ISBN 0-917256-20-4). , 1985).

However, neither of the above documents teaches or discloses that the extract of Cauliflower fruit of the present invention has antioxidant, anti-inflammatory and whitening activity.

Therefore, the present inventors have identified the antioxidant, anti-inflammatory and whitening activity of cedar barley fruit extract through DPPH free radical inhibitory activity, reducing power, xanthine oxidase inhibitory activity, platelet aggregation inhibitory activity, nitrite formation inhibitory activity and tyrosinase inhibitory activity experiments. The invention has been completed.

 The present invention is an external skin pharmaceutical composition for the prevention and treatment of skin diseases and inflammatory diseases caused by oxidative stress and the skin disease and inflammation caused by oxidative stress, containing the extract of C. barberry fruit having antioxidant, anti-inflammatory and whitening activity as an active ingredient. Its purpose is to provide a cosmetic composition for the prevention and improvement of diseases.

In order to achieve the above object, the present invention is an external skin pharmacy for the prevention and treatment of skin diseases and inflammatory diseases caused by oxidative stress containing the extract of Elaeagnus multiflora fruit having an antioxidant, anti-inflammatory and whitening activity as an active ingredient To provide a composition.

The pharmaceutical composition comprises a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation.

In addition, the present invention has an antioxidant, anti-inflammatory and whitening activity,Elaeagnus multiflora) It provides a cosmetic composition for the prevention and improvement of skin diseases and inflammatory diseases caused by oxidative stress containing fruit extract as an active ingredient.

      The cosmetic composition includes a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, the formulation of the pack.

The extract as defined herein is at least one solvent selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, hydrous butylene glycol, propylene glycol, hydrous propylene glycol, hydrous glycerin It is characterized by being extracted.

Skin diseases due to oxidative stress as defined herein include skin diseases such as skin aging, wrinkles, blemishes, freckles, dermatitis, acne and the like, and inflammatory diseases as defined herein include acne, eczema and the like.

Hereinafter, the present invention will be described in detail.

The berry tree fruit extract of the present invention is dried berry tree fruit (fruit) by 3 to 20 times the weight (kg), preferably 5 to 10 times by weight of water, ethanol, methanol, propanol, butanol, acetone, ethyl 30 minutes to 2 days at 10 to 200 ° C., preferably 20 to 100 ° C., preferably 24 to 36, with one or more solvents selected from the group consisting of acetate, hexane, butyl butyl glycol, propylene propylene glycol and glycerol It can be obtained by extracting for a time or by extracting for 2 hours to 15 days, preferably 1 to 3 days at 5 to 37 ℃, preferably room temperature.

The green barley fruit extract of the present invention exhibits DPPH free radical inhibitory activity, reducing power, xanthine oxidase inhibitory activity, platelet aggregation inhibitory activity, nitrite production inhibitory activity and tyrosinase inhibitory activity.

As confirmed above, the composition comprising the active extract of the berry tree fruit, which shows excellent activity and no toxicity and proved to be very high in human safety, is used for the prevention and treatment of skin diseases and inflammatory diseases caused by oxidative stress. It can be usefully used.

The extract can be used in 0.001 to 90% by weight, preferably 0.01 to 10% by weight based on the total weight of the skin external pharmaceutical composition or cosmetic composition.

The external skin pharmaceutical composition containing the extract of the present invention is a skin external preparation having an antioxidant, anti-inflammatory and whitening effect, such as cream, gel, patch, spray, ointment, warning agent, lotion, linen, pasta or cataplasma. It may be prepared and used as a pharmaceutical composition in the form of external preparations for skin, but is not limited thereto.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the extract of the present invention can be used in various ways, such as cosmetics and face wash having an antioxidant, anti-inflammatory and whitening effect.

Products to which the composition can be added include, for example, cosmetics such as lotion, skin, lotion, nourishing lotion, nourishing cream, massage cream, essence and pack, and cleansing, face wash, soap, treatment, essence, etc. have.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, lauric acid hexyl, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, an alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1. Extraction solvent Garden tree  Preparation of Fruit Extract

The garden barley trees (fruits) collected directly from a farm in Gyeongsan, Gyeongbuk, were finely pulverized for 1 week. Using Soxhlet extractor, 200 ml of extraction solvent per 40 g of sample was added, and extracted at room temperature for 24 hours, 0.39 g of petroleum ether extract (hereinafter referred to as PE), and chloroform extract (chloroform extract, Hereinafter referred to as CE) 0.38g, ethyl acetate extract (hereinafter referred to as EE) 0.37g, butanol extract (hereinafter referred to as BE) 1.24g and water extract (hereinafter referred to as WE) 9.71 g were extracted, and each extract was concentrated under reduced pressure with a vacuum concentrator (BRotavapor R-114, Switzerland) at 50 ° C or lower. The residue of PE was diluted in a solvent of dimethyl sulfoxide / methanol / chloroform (3/1/1), and CE, EE, BE and WE were diluted in dimethyl sulfoxide (DMSO), respectively. It was used as a sample of the experiment. Each sample was stored at 4 ℃, it was used diluted by concentration before the experiment.

Experimental Example  One. DPPH Radical Scavenging power ( DPPH  radical scavenging activity

DPPH radical scavenging activity was determined by modifying the method of Hwang et al., Journal of Natural Products, 64, pp8284, 2001. 10ul of extract was dispensed into 96-well microplate (96-well microplate; SPL, Suwon, Korea) and 200ul of DPPH (1.1-diphenyl-2-picrylhydrazyl; dissolved in 99% ethanol; 100uM) was added. After reacting the sample at 37 ° C. for 30 minutes, the absorbance was measured at 517 nm using a VERSA max microplate reader (Molecular Devices Corp., Sunnyvale, Calif.). Scavenging activity of DPPH free radicals was calculated according to the following equation. The 50% inhibitory effect concentration (IC ₅₀ ) of the green barley water required to show the 50% inhibitory effect of DPPH free radicals was calculated based on ascorbic acid as a control.

Sd : absorbance of the sample to which DPPH was added

Se : absorbance of the sample to which ethanol is added

C : absorbance of DPPH solution

As shown in FIG. 1, all extracts of the cedar barley fruit showed lower antioxidant effects than the control group ascorbic acid, but showed different DPPH radical scavenging ability as the concentration increased. It decreased in the order of CE>EE>PE>WE> BE at the concentration of 10 mg / ml. DPPH radical 50% inhibition effective concentration (IC ₅₀₎ of ascorbic acid was the control group, 0.117mg / ml, 50% inhibition effective concentration (IC ₅₀₎ at the time when processing gardening linden extract of the present invention at a concentration of 10mg / ml CE 2.553mg / ml, EE 3.723mg / ml and PE were 6.632mg / ml and showed little effect in WE and BE.

Experimental Example  2. Reducing power verification experiment

Reducing power of the fruit extracts of the cornflower tree was measured by slightly modifying the method of Kuda et al., Japan.Journal of Food Composition and Analysis , 18 , pp625-633, 2005. 0.2 ml of 0.2 M phosphate buffer solution (pH 6.6) and 1% potassium ferricyanide (potassium ferricyanide) per 0.2 ml of the sample were mixed and reacted at 50 ° C. for 20 minutes. 0.2 ml of 10% trichloroacetic acid was added to each of the reacted samples, followed by mixing and centrifugation at 1,000 × g for 10 minutes. The supernatant (0.125 ml) was mixed with the same amount of distilled water (DW) and 0.02 ml of 0.1% ferric chloride (FeCl ₃ ) was added to a 96-well microplate (SPL, Suwon, Korea). The absorbance was then measured at 700 nm using a VERSA max microplate reader (Molecular Devices Corp., Sunnyvale, Calif.). The high absorbance of the reacted samples showed a very high reducing power, and the reducing power of the sample was calculated based on ascorbic acid as a control.

The effect on the reducing power of the various solvent extracts of green barley water is shown in Figure 2, showing similar results to the DPPH radical scavenging ability of Experimental Example 1. At 500 ug / ml of each extract, the reducing power decreased in the order of CE> EE> WE> PE> BE, and the control group, ascorbic acid, showed a higher reducing power than that of the extract of Cauliflower.

Experimental Example  3. Xanthine oxidase ( xanthine oxidase Inhibitory Activity Confirmation Experiment

Xanthine oxidase (XO) activity was measured by uric acid production of xanthin (Chang et al., Anticancer Res., 14 , pp501-506, 1994). To 100ul of sample, add 100ul (1.3nM) of xanthine solution added with 40ul of xanthine oxidase (0.4 unit / ml) and add 50mM phosphate buffer pH 7.4 to 2ml. Was added and reacted at room temperature for 3 minutes. And at 295 nm, the absorbance of the sample addition and the reagent addition without measurement was measured. After adding each sample (100ul) for each concentration, the reaction reagent was added, and the amount of xanthine oxidase reduction and 50% inhibitory effect concentration (IC ₅₀ ) were produced by the effect of producing uric acid based on ascorbic acid as a control group. ) Was measured and calculated.

The xanthine oxidase inhibitory effect of the fruit of the barley tree fruit is shown in FIG. 3. Among the extracts, the highest concentration of 50% inhibitory effect (IC ₅₀ ) was 68.76 ug / ml in EE, and xanthine oxidase inhibitory effect was found even at a much lower concentration than 405.87 ug / ml of the control group ascorbic acid. However, the other extracts showed xanthine oxidase inhibitory effect at a very high content.

Experimental Example  4. Antiplatelet  Anti-platelet aggregation activity confirmation experiment

Platelet aggregation was tested in vitro using a coagulation meter (whole blood aggregometer, chronolog, Havertown, PA). 0.4 ml of washed platelets were placed in a coagulation tube containing a final concentration of 1.3 mM calcium chloride (CaCl ₂ ), stirred at 1,000 rpm at 37 ° C., and 0.5 u / ml of thrombin was added to induce coagulation. Induced and observed the reaction caused by the change in light transmission for 5 minutes. Aggregation of each extract was carried out for 1 minute (37 ° C., 1,000 rpm) using pretreated washed platelets, and aggregation occurred immediately after thrombin. Aggregation measuring tube without the extract was used as a control and represented by calculating the inhibitory effect (%) unit using the following equation (2).

A: maximum cohesion of the control group

B: maximum cohesion of the sample pretreated with the extract

Compared with the control group, the aggregation inhibitory effect was 19.29% only in CE at 250 ug / ml, indicating a very low aggregation reaction. (See Figure 4)

Experimental Example  5. Experiment to confirm inhibition of nitrite oxide (NO production)

RAW 264.7 cells (1 × 10 ⁶ cells / ml, ATCC, USA) were pretreated with 30 mL of each extract concentration (125, 250, 500 ug / ml) for 30 minutes and then lipopolysaccharide (LPS) at 1 ug / ml for 24 hours. Reacted. Nitrite measurement was performed by dispensing 50ul of cells reacted in a 96-well flat-bottom plate, adding 100ul of Greries reagent to each well, and then room temperature. It was reacted for 10 minutes in (light blocking). Absorbance was measured at 540 nm using a VERSA max microplate reader (Molecular Devices Corp., Sunnyvale, Calif.), And the nitrite of each sample was measured by sodium nitrite (standard); 0-100 μM cell culture medium).

The inhibitory effect on the production of nitric oxide (NO) by the extract of cedar barley extract of various solvents induced by lipopolysaccharide (LPS) is shown in FIG. 5. In the present experiments, all extracts of cedar barley inhibited the production of lipopolysaccharide (LPS) -induced nitrite by the concentration-dependent method. Especially, CE showed almost 100% inhibition effect at 500 ug / ml. It was. CE, PE, and EE were effective even at low concentrations (≤250ug / ml), while BE and WE showed inhibitory effects of 43.08% and 44.04%, respectively, at concentrations of 500ug / ml.

Experimental Example  6. Whitening effect ( Tyrosinase  Inhibitory effect; Tyrosinase  Inhibitory activity confirmation experiment

The oxidase activity of tyrosinase on dopa was determined according to the method of Baek et al., Kor . J. Microbiol . Biotechnol ., 31 , pp135-139, 2003. 150 μl of L-dopa (1.5 mM 38 μl and 100 mM phosphate buffer pH 6.5) and 10 μl of mushroom tyrosynase at 10 μl of 2100 units / ul were used in 96-well microplates (SPL, Suwon). , Korea) and 3ul each sample was mixed and reacted for 1 hour at 25 ℃. The inhibitory activity of the sample was calculated according to Equation 3 below by measuring absorbance at 490 nm using a VERSA max microplate reader (Molecular Devices Corp., Sunnyvale, Calif.).

A: absorbance before the reaction of the sample addition

B: absorbance after reaction of sample addition

C: Absorbance before reaction of sample-free addition

D: absorbance after reaction of sample-free addition

Tyrosinase activity according to the concentrations of the extracts of garden barley is shown in Table 1 below. All extracts except WE increased dose-dependent inhibitory effect on tyrosinase activity. WE showed little inhibition of tyrosinase activity, but especially EE was 107.94 ± 2.8% at 10mg / ml and 43.70 ± 0.04% at 3mg / ml, which is very strong tyrosinase activity compared to other extracts Inhibitory effect was shown.

Concentration (mg / ml) Inhibition rate of tyrosinase activity (%) PE CE EE BE WE One 0.00 ± 0.00 3.19 ± 3.50 12.77 ± 16.82 0.00 ± 0.00 1.59 ± 8.51 3 26.30 ± 4.64 ^b 3.89 ± 1.46 ^c 43.70 ± 0.04 ^a 2.73 ± 0.91 ^c 8.56 ± 4.21 ^c 10 46.54 ± 2.54 ^b 45.65 ± 9.13 ^b 107.94 ± 2.80 ^a 24.27 ± 4.84 ^c 5.51 ± 3.86 ^d

Experimental Example  7. GC -MS analysis

Phenolic compounds isolated from medicinal and herbal plants are important natural antioxidants and are well known for their medical effects such as anti-inflammatory, inhibition of platelet aggregation, prevention of atherosclerosis and anticancer effects (Kahl, R., Oxidants and antioxidants., 92101, 1991) In this experiment, we searched the compounds of lysed barley tree extracted from various solvents by analytical methods using GC-MS, and phenol and 2,4-bis (1) in CE and EE. Phenolic compounds, such as (1-dimethylethyl), were searched for the amount thereof. This may explain the antioxidant, anti-platelet aggregation effect of CE or EE, which is relatively lower than the control.

In this experiment, the instrument was equipped with a Hewlett-Packard 6890 gas chromatography (GC) attached with an HP-5 MS column (length: 30 m, inner diameter: 0.25 mm, stationary phase thickness: 0.25). Hewlett-Packard 5974 Mass Spectrometer (MS) was used. The mobile phase gas was a helium gas with a flow rate of 0.7 ml / min, and the injection method was a split injection method of 50: 1 per 1ul of sample. The initial temperature of the column was 70 ° C. The temperature was raised to 300 ° C at a rate of 7 ° C / min and maintained for 10 minutes. The injector was set to 200 ° C and the detector to 300 ° C. . Compounds in the samples were identified by comparison with the mass spectrometry results of the standard. (See Table 2, Table 3)

compound Length of stay (minutes) % Of extract Phenol, 2,4-bis (1,1-dimethylethyl) 13.676 6.84 Hexadecanoic acid, 2-hydroxy-1- (hydroxymethyl) ethyl ester 27.389 10.16 Dotriacontane 29.393 2.12 Octadecanoic acid, 2-hydroxy-1- (hydroxymethyl) ethyl ester 29.619 49.90 Eicosane 30.401 2.02 Tranylcypromine, pentafluorobenzoyl ester 31.17 1.95 Benzoflex 32.792 6.71 Cyclotrisiloxane, hexamethyl 36.1 3.49

compound Number of minutes stayed % Of extract 2,3-butanediol 2.36 5.89 Octane, 4-methyl 2.63 2.00 2-Propanol 2.94 6.56 2-hexanol 2.99 3.08 Octane, 3,3-dimethyl 5.15 1.39 Undecane, 5-methyl 5.78 10.76 Dodecane, 2,6,10-trimethyl 5.88 2.57 Octane, 6-ethyl-2-methyl 6.63 5.72 Heptadecane 6.74 2.03 Pentadecane 10.35 2.30 Phenolic, 2,4-bis (1,1-dimethylethyl) (2,4-bis (1,1-dimethylethyl)) 15.21 5.99

The experimental results were analyzed using the SPSS 12.0.1 program for Windows (SPSS Inc, Chicago, IL, U.S.A.) and expressed as mean ± standard deviation (S.D). One-way ANOVA verifies significant differences between groups with Student-Newman-Keuls (SNK) or Dunnett's multiple comparison test, and the significance at p <0.005. It was.

It describes an example of the formulation of the cosmetic composition comprising the fruit of the barley tree fruit extract of the present invention, the present invention is not intended to limit this, it is intended to explain in detail only.

Formulation example  1. Preparation of Hydrophilic Ointment

Raw material weight%   Foliage Fruit Extract 9.00   White vaseline 36.00   Stearyl alcohol 31.00   Ethyl (or methyl) p-oxybenzoate a very small amount   Propylene glycol 19.00   Sodium lauryl sulfate 2.40   Propyl p-oxybenzoate a very small amount system 100.00

Formulation example  2. Flexible Cosmetics skin Manufacturing

Raw material weight%   Foliage Fruit Extract 2.0 1,3-butylene glycol 2.00 glycerin 3.00 Oleyl alcohol 2.00 Polysorbate 20 1.00 ethanol 6.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  3. Milk croissant  Produce

Raw material weight%   Foliage Fruit Extract 5.0 1,3-butylene glycol 4.00 glycerin 3.00 Oleyl alcohol 0.05 Polysorbate 20 1.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  4. Manufacturing of Nutritional Cream

Raw material Formulation Example 4   Foliage Fruit Extract 2.0 Vaseline 5.00 Liquid paraffin 30.00 Beeswax 3.00 Polysorbate 60 1.00 Sorbitan sesquioleate 1.00 1,3-butylene glycol 5.00 glycerin 5.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  5. Massage  Manufacture of cream

Raw material weight%   Foliage Fruit Extract 7.0 glycerin 5.00 Triethanolamine 0.50 Tocopheryl acetate 1.00 Liquid paraffin 5.00 Squalane 5.00 Macadamia Nut Oil 15.00 Polysorbate 60 1.00 Sorbitan sesquioleate 1.00 Carboxy vinyl polymer 0.20 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

As described above, the extract of C. barberry fruit of the present invention exhibits antioxidant, anti-inflammatory and whitening activity such as DPPH free radical inhibitory activity, reducing power, xanthine oxidase inhibitory activity, platelet aggregation inhibitory activity, nitrite production inhibitory activity and tyrosinase inhibition. Therefore, it can be used as a pharmaceutical composition for the prevention and treatment of skin diseases and inflammatory diseases caused by oxidative stress and the cosmetic composition for the prevention and improvement of skin diseases and inflammatory diseases caused by oxidative stress.

Claims (6)

A skin external pharmaceutical composition for the prevention and treatment of inflammation-related skin diseases containing an anti-inflammatory activity of Elaeagnus multiflora fruit petroleum ether, chloroform or ethyl acetate soluble extract as an active ingredient. Cosmetic composition for the prevention and improvement of inflammation-related skin diseases containing anti-inflammatory activity Elaeagnus multiflora fruit petroleum ether, chloroform or ethyl acetate soluble extract as an active ingredient. delete delete delete delete

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