KR101143820B1 - Cosmetic composition comprising an extract of Aster ageratoides Turcz. var. ageratoides having whitening activities - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of Aster ageratoides Turcz. Var. Ageratoides as an active ingredient, and specifically, exhibits excellent tyrosinase inhibitory activity in an in vitro experiment, and tests in animal cells. It can be usefully used as a cosmetic composition showing a melanin synthesis inhibitory activity in.

Description

Cosmetic composition containing the extract of black wormwood exhibiting whitening activity {Cosmetic composition comprising an extract of Aster ageratoides Turcz. var. ageratoides having whitening activities}

The present invention relates to a cosmetic composition for whitening containing the extract of black wormwood as an active ingredient.

One of the areas of greatest concern for women in East Asia, especially women in Korea, Japan and China, is skin whitening. "Skin whitening" means the production of pigments in the skin due to environmental changes such as ultraviolet rays, stress, hormone secretion and pregnancy. It can be said that it is a concept that prepares the skin color change that occurs excessively. The most important factor in this regard is melanin, a skin pigment. Melanin is synthesized in melanocytes (melanocytes), which are pigment cells in the basal layer of the epidermis, and are transferred to surrounding keratinocytes to determine skin color. Originally, melanin is a black pigment found in animals, plants, microorganisms, etc. It is not essential for growth or development, but it is a substance that enhances the viability and competitiveness for any environment. Especially, melanin in animals is associated with skin disease and malignant melanoma (melanomas). In addition to dopa melanin produced from tyrosine by tyrosinase, 1,8-dihydroxynaphthalene melanin (DHN melanin; 1,8-Dihydroxynaphtalene melanin), ortho-diphenol melanin (GDHB melanin; o- diphenol melanin), catechol melanin and the like have been reported (Bell AA and Wheeler MH, Annu. Rev. Phytopathol. , 24, pp 411-451, 1986). Melanin is an unusually stable pigment found in living organisms, insoluble in almost all solvents, and difficult to extract or chemically handle. Because of this, there are many unknowns about the chemical aspects of melanin and the progress of research is sluggish.

The process of melanogenesis is clearly shown to occur in organelles called melanosomes of specially differentiated cells called melanocytes.

That is, the skin color is determined according to the content, distribution, and the like of melanin and is related to the number and distribution of melanosomes generated in melanocytes and released to the outside of cells. Hyperpigmentation of the skin can be caused by a number of factors, including hormonal abnormalities, genetic diseases, and UV irradiation after the inflammatory response of the skin, mainly due to melanin pigment synthesis abnormalities and distribution abnormalities. The main function of melanin is to remove oxygen radicals to protect the skin from the damage caused by melanin, which means that it has an effective response system to protect skin from physical and chemical toxic substances.

The prevention of pigmentation in the skin can be studied in three ways. 1) development of an inhibitor of tyrosinase synthesis or an antagonist to the substrate of tyrosinase to regulate tyrosinase activity, the main enzyme of melanin synthesis, and 2) deterioration of the function of melanocytes, a site of melanin biosynthesis in animals 3) Development of 3,4-dihydroxyphenylalanine-reducing substance and melanin-forming enzyme 1 tyrosinase and dopachrome to prevent oxidation of 3,4-dihydroxyphenylalanine (DOPA) DOPAchrome tautomerase, a second enzyme that catalyzes the conversion of (DOPAchrome) to 5,6-dihydroxyindole-2-carboxylic acid (DHICA; 5,6-Dihydroxyindole-2-carboxylic acid) Of a third enzyme catalyzing the conversion of, 6-dihydroxyindole-2-carboxylic acid to indole-5,6-quinone-2-carboxylic acid Activity control and the like are attracting attention.

Excessive melanin deposition is a cause of pathological problems such as skin, but is recognized as a cosmetic problem such as spots, freckles, spots and blotch, thus increasing interest in whitening agents that can inhibit melanin formation. There is much research going on.

Tyrosinase (tyrosinase; monophenol, dihydroxy-L-phenylalanine: oxygen oxidoreductase, EC 1.14.18.1) is a copper-containing enzyme that uses a wide range of phenolic compounds as a substrate, and L-3,4- using tyrosine as a substrate. Melanine is produced by polymerization of each product through successive enzymatic oxidation and oxidation to produce dihydroxyphenylalanine (L-3,4-dihydroxyphenylalanine) and transfer it back to dopaquinone (L-DOPAquinone). melanin) is biosynthesized. The mitosis of melanocytes is activated by ultraviolet rays, and melanocytes are activated. In activated melanocytes, the synthesis of tyrosinase is promoted, which promotes melanin production. Cause. Abnormally over-producing melanin produces blemishes and freckles, whereas less-producing melanin causes skin lesions such as alum. Pigmentation is caused by various stimuli such as ultraviolet rays and inflammation from the outside, and the degree of pigmentation is the amount of the reaction of melanocytes present in the base layer of the epidermis. Central to the pigmentation mechanism is tyrosinase.

In modern times, according to the socio-cultural trend that emphasizes beauty and health, cosmetics have become more than just clothing, becoming a necessity to maintain healthy skin. In addition, according to the nature-oriented and environmentally friendly consumption trend, the development of functional cosmetics having a functional using a variety of natural materials are actively made. The cosmetics industry is a typical fine chemical industry based on basic science and applied technologies such as chemistry, life sciences, pharmacy, and physiology, and as the cosmetics industry has recently developed rapidly, attention has been paid to the efficacy of the skin safety of products.

Analysis of patents on functional cosmetics filed in Korea focuses on active ingredients that effectively represent the functions of whitening, wrinkle improvement and UV protection, and is especially focused on the Asian region, which craves white skin. Research has been actively conducted on the hypoallergenic whitening, high-efficiency raw materials and stabilization of the raw materials. 44% of natural plant extracts, 25% of new compounds, and 31% of other substances such as vitamin C derivatives known as whitening ingredients were found for each whitening active ingredient. However, it is of great interest all over the world and it is considered very important to find the active substance by checking its functionality.

Currently, ascorbic acid, kojic acid, arbutin, hydroquinone, oily licorice extract, and various plant extracts are used as whitening cosmetic materials. Among these, kojic acid chelates copper ions present in the active site of tyrosinase, which inhibits enzymatic activity. While it has good performance, it is not suitable for use because it has problems in stability and economical efficiency when formulated in cosmetics. In addition, hydroquinone (hydroquinone) is irritating to the skin and is currently not used in cosmetics due to safety issues. In addition, research has been conducted to find whitening active ingredients among natural products, especially plants. Among them, many plant extracts and herbal extracts such as lettuce, licorice, peony, cinnamon, red ginseng, brown root, donkey, bark skin, halves and aloe are tyrosinase ( tyrosinase) inhibitory activity has been found, but they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration potential, there is a limitation that the whitening efficacy is not obvious.

The present invention is to provide a cosmetic composition containing the extract of black wormwood.

The present invention is to provide a cosmetic composition excellent in tyrosinase inhibitory activity.

The present invention is to provide a cosmetic composition useful as a composition for skin whitening.

As a preferred embodiment, the present invention provides a cosmetic composition for whitening containing the extract of black wormwood as an active ingredient.

In the above embodiment, the locust wormwood extract is ground, including flowers, branches, stems, leaves, immature fruit, fruit; Rhizome; And one or more extracts selected from the roots.

In the above embodiment, the extract may be extracted with one or more mixed solvents selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, ether and chloroform .

In the above embodiment, the cosmetic composition may be one containing 0.001 to 90% by weight of the extract of black wormwood.

In this embodiment, the cosmetic may be a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, oils, waxes, powders, sprays, soaps, cleansing, packs, foundations and hair cosmetics. .

delete

delete

delete

delete

The cosmetic composition of the present invention contains an extract of blackberry wormwood which shows excellent tyrosinase inhibitory activity in vitro and shows excellent melanin synthesis inhibitory activity in experiments using animal cells. Can provide.

Hereinafter, the present invention will be described in detail.

Aster ageratoides Turcz. Var. Ageratoides is a chrysanthemum family, and the herbal name is Sanbaekguk (山 白菊). The country of origin is Korea, China, Russia, Northern India, etc., and its origin is Korea. It grows in the form of perennials on the fields and forest edges of the whole country. October is characterized by sharp tip of leaves, 10 ~ 14cm long, 3 ~ 6cm wide, rough surface, serrated edge and fine hair on stem and leaf. Its tender leaves are used for food, and young leaves are eaten raw or lightly boiled and eaten. They are boiled in hot water and dried in the sun to be used as mung bean sprouts. Ingredients include kaempferol, quercetin, quercetin rhamnoside, and the reported component studies are Aster ageratoides Turcz. From water-ethanol extracts of buds, p-coumaroylquinic acid, caffeoylquinic acid, feruloylquinic acid, dicapoylquinic acid and diferulo Three isomers, p-coumaroyl-caffeoylquinic acid, p-coumaroyl-feruloylquinic acid and caffeoyl-, respectively, for diferuloylquinic acid A total of 33 chlorogenic acids were isolated from each of six isomers of caffeoyl-feruloylquinic acid (Clifford MN et al, Phytochem.Anal ., 17 (6), pp384-393). , 2006). Yan FL et al. Also reported that Aster ageratoides var. Two new pentacyclic triterpenoids from pilosus , 2-alpha, 3-beta-16alpha-trihydroxyl-24alpha-al-olian-12-ene-28-oic acid [2beta , 3beta, 16alpha-trihydroxyl-24alpha-al-olean-12-en-28-oic acid (1)] and 2-beta, 3-beta-dihydroxyl-16-O-beta-D-glucopyranose- 24-alpha-al-olian-12-ene-28-oic acid [2beta, 3beta-dihydroxyl-16-O-beta-D-glucopyranose-24alpha-al-olean-12-en-28-oic acid (2 ) And two known pentacyclic triterpenoids were isolated and tested for antimicrobial and antitumor activity (Yan FL et al, Pharmazie , 59 (11), pp882-884, 2004). In addition, the separation of crotocorylifuran and oplopanone from Aster ageratoides were identified (Ahmed AA et al, Pharmazie , 57 (8), 567-569). The pharmacological action of the crab wormwood has not been studied much, and there are only reports on anticancer, antibacterial and anti-follicular effects (Kim Chang-jin, et al . , 39 (5), pp409-413, 1996).

In the oriental medicine, the rooted outpost is called Sanbaekguk, and it is known to cure gusts, blues, detoxification, mudslides, and the sea, and to treat colds caused by winds, tonsillitis, bronchitis, serpentine, and bee stings on bees (Korean medicinal plants in Korea). , JH Chang, Hakchangsa, pp491, 2000).

As you can see, there are a variety of functions in the craggy worms, but scientifically proven results are limited. In addition, there is no patent related to the physiological activity function of black wormwood wormwood, only the black wormwood mention is mentioned in the invention title 'Chrysanthemum flower wormwood' (Korean Patent Publication No. 2002-0046108) and the content related to the whitening activity of black wormwood wormwood No teaching has been disclosed.

Therefore, the present inventors have searched for a potent substance from 200 kinds of natural products to solve the problems of the conventional whitening raw materials and find more excellent whitening effect, so that the extract of blackberry wormwood has excellent tyrosinase inhibitory activity. It was confirmed through intracellular and intracellular experiments, and the whitening efficacy of the extracts of black wormwood is very strong, and since these natural products have been used by humans for a long time, they are considered to contain relatively safe ingredients to humans. It is believed that it can be developed as a functional material.

As a preferred embodiment, the present invention provides a cosmetic composition containing the extract of Aster ageratoides Turcz.var.ageratoides as an active ingredient.

The Cabbage Worm Extract can be obtained as follows.

After washing, the mugwort worms are added with a solvent that is about 1 to 30 times, preferably about 1 to 15 times, the weight of the sample after washing, at about 10 to 100 ° C. for about 1 hour to 4 days, preferably 2 Filtration (or filtration after centrifugation), concentration under reduced pressure, and lyophilization using extraction methods such as boiling water extraction, reflux circulation extraction, dipping extraction or pressure extraction for a period of time to 2 days to obtain the extracts of the present invention Can be.

The wormwood wormwood is ground, including flowers, branches, stems, leaves, immature fruit, fruit; Rhizome; And one or more extracts selected from the roots.

The solvent may be one or more mixed solvents selected from the group consisting of water including purified water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, ether, and chloroform.

As a result of measuring the tyrosinase inhibitory activity of the extract of Cabbage wormwood obtained by the extraction method, the whitening effect is excellent and can be usefully used as a cosmetic composition for whitening.

In addition, the wormwood wormwood has been used for a long time as a herbal medicine and edible extracts of the present invention extracted from them also have no problems such as toxicity and side effects, it is safe even for long-term use because it has been proved to be a non-irritating sample in the skin patch test Can be used.

The cosmetic composition of the present invention is 0.001 to 90% by weight of the extract of the locust mugwort with respect to the total weight of the cosmetics, in consideration of the improvement of the whitening effect and skin sensitivity through inhibition of tyrosinase activity and inhibition of melanin production in melanocytes. Preferably 0.001 to 70% by weight, most preferably 0.001 to 50% by weight.

The cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. The salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherolvitamin E nicotinic acid, DL-pantothenyl alcohol, D-pantothenyl) Alcohols, pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, pit spingosine, sphingolipid lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristin, butyl myristic acid, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearic acid, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (caprylic capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, cetyl lactate, octyl lactate, citric acid Triethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate Dioctyl sebacate, stearic acid cholesterol, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, phosphate oleate , 12-stealoylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-steal Ester type | system | groups, such as a loyl hydroxystearic acid isostearyl, etc. are mentioned.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone eicosene copolymers, polyvinylpyrrolidone hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE®POP (Polyoxyethylene® Polyoxypropylene) Copolymer, POE®POP Alkyl Ether, Polyether Modified Silicone, Lauric Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, carbon black and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl, such as Nε-lauroyl-L-lysine, N-ε-palmitolysine, N-α-paratoylol nitin, N-α-lauroyl arginine, and N-α-cured fatty acid acyl arginine Basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate and cinnamic acid benzyl , Paramethoxy cinnamic acid 2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urokanoic acid Ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone, tetra Hydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1,3 , 5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokichiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably it is mix | blended with 0.01 to 3 weight%.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Ingredients included in the cosmetic composition of the present invention as an active ingredient, including components commonly used in cosmetic compositions in addition to the extract of black wormwood, for example, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings Conventional adjuvants and carriers may be included.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, oils, waxes, powders, sprays, soaps, It may be formulated as a cleansing, pack, foundation, hair cosmetic, and the like, but is not limited thereto.

Specific examples of the cosmetic of the present invention include skin lotion, skin softener, skin toner, astringent, nourishing lotion, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, cold cream, moisturizing cream, hand cream, foundation, essence , Nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, aftershave cream, sunburn prevention cream, suntan oil, hair shampoo, hair rinse, hand cream, foundation, body lotion, body cleanser, etc. .

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is cleansing, i.e., surfactant-containing cleansing, as a carrier component, an aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Below, The invention is illustrated in detail by the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Reference Example 1. Animal cell line culture

Melan-a cell line, melanosite derived from mouse, the animal cell line used in the present invention, was distributed from Dr. DC Bennett, University of London, UK, 2 mM glutamine, 100 U / ml penicillin G, 100 μg / ml streptomycin (streptomycin, Gibco, USA), 10% fetal bovine serum (FBS, Gibco, USA), 100 nM TPI (TPA, 12- Experiment with subculture in RPM2 1640 (Gibco, USA) medium containing O-tetradecanoylphorbol-13-acetate, Sigma, USA) at 37 ° C. in a 10% carbon dioxide and 90% air composition CO ₂ incubator It was used for.

Preparation Example 1 Preparation of Black Bean Worm Extract

Cabbage wormwood used in the present invention is a ground portion including flowers, branches, stems, leaves, immature fruit, fruit; Rhizome; Roots were harvested and the selected one or more assayed test materials were washed and cut. The extract obtained by adding 80% ethanol at a ratio of 1: 5 (w / v) to 1 kg of the sample, C. locust, and refluxing three times at room temperature for 48 hours after mixing, was prepared as No. 2 (Advantec TOYO, Japan), and the resultant was concentrated under reduced pressure with a rotary vacuum evaporator (Heidolph VV2011, Switzerland) at 50 ° C. and freeze-dried to obtain an ethanol extract of brown rhododendron, and stored at -20 ° C. to inhibit tyrosinase activity. Used as sample for analysis.

Experimental Example 1. Measurement of tyrosinase inhibitory activity of the extract in vitro )

Tyrosinase (monophenol monooxygenase, EC1.14.18.1, Sigma, USA) Determination of inhibitory activity was carried out by Masamoto Y et al., Planta Med., 40 (a method for measuring dopaquinone produced by the action of tyrosinase by colorimetry). 4), pp361-365, 1980) was modified with some modifications.

0.6 ml of 100 mM sodium phosphate buffer (pH 6.8) and 15 μl of mushroom tyrosinase (mushroom tyrosinase, 40 units, Sigma, USA) and dimethylsulfoxide (Dimethylsulfoxide) The extract of Blackberry Wormwood of Example 1 was added so as to have a final concentration of 50, 100, 250 μg / ml, and corrected with distilled water so as to have a final 0.86 ml. The mixture was left at room temperature for 5 minutes, and then 0.14 ml of 3.4 mM diphenylalanine (DOPA, L-3,4-dihydroxyphenylalanine) was added as a substrate, followed by spectrophotometer (U-3000, Hitachi, Japan) at 475 nm. The amount of change in dopaquinone produced in the reaction solution was measured at room temperature for 10 minutes (time scanning), and compared with the absorbance of the control group in which the solvent was added instead of the sample solution to obtain the tyrosinase inhibition rate (%). It is shown in Table 1 and FIG.

As shown in Fig. 1, the concentration of tyrosinase inhibitory activity was determined as a result of scanning the temporal conversion of dopa (DOPA) to dopaquinone by the addition of 50, 100, 250 μg / ml Indicated.

In detail, as shown in Fig. 2, the extract of Blackberry wormwood inhibited tyrosinase activity of 31.7% when 50 μg / mL was added, 50.8% when 100 μg / mL was added and 60.3% when 250 μg / mL was added. From the results, it can be confirmed that the extract of the horseshoe wormwood has excellent tyrosinase inhibitory activity.

Black Bean Worm Extract Treatment Concentration (μg / mL) Tyrosinase Inhibitory Activity (%) 50 31.7 100 50.8 250 60.3

Experimental Example 2 Measurement of Melanin Synthesis Inhibitory Effect of the Extract of Caesar Worm Extract (Test Using Animal Cell Line)

Tyrosinase inhibitory efficacy in animal cell lines was measured by measuring the melanin production inhibitory activity of melanocytes, a melanosite cell line of mice treated with black wormwood extract. Melanin quantification was used by modifying the method of Hosoi J et al., Cancer Res. , 45 (4), pp1474-1478, 1985.

Melanocytes, melanocytes (melanocytes) of the mouse were dispensed in 6-well plates at 3 × 10 ⁴ cells / well and cultured for 48 hours to attach cells. Crunch Worm extract was treated to a final concentration of 10, 20, 30 μg / ㎖ and incubated for 5 days. The cultured cells were washed twice with phosphate buffer (DPBS; Dulbecco's Phosphated Buffered Saline) containing no calcium chloride (CaCl ₂ ) or magnesium chloride (MgCl ₂ ) and treated with trypsin (Gibco, USA) to separate the cells. The same number of cells were collected per group. The cell precipitate was washed with phosphate buffer (DPBS; Dulbecco's Phosphated Buffered Saline) containing no calcium chloride (CaCl ₂ ) or magnesium chloride (MgCl ₂ ) and 1 normal concentration with 100 μl of 10% dimethyl sulfoxide (DMSO). Suspended in (N) sodium hydroxide (NaOH) solution and treated for 30 minutes at 60 ℃ and measured the absorbance at 475 nm to convert the amount of melanin from the standard curve made of synthetic melanin is shown in Table 2, Figure 3 It is shown in Figure 4 that the brown melanin is reduced by the extract extract.

As shown in Fig. 3, the melanin amount of the melanin according to the above test method as the melan-A cell line of the mouse cultured by adding the extract of the locust mugwort of the present invention to a final concentration of 10, 20, 30 μg / ㎖ As a result, the tyrosinase inhibitory activity was inhibited, and 15.9% of the extracts of M. aeruginosa were inhibited by 15.9%, 75.3% by the addition of 20 μg / ml, and 82.4% melanin synthesis by the addition of 30 μg / ml. From the above results, IC ₅₀ according to the melanin synthesis inhibitory activity of the extract of the locust mugwort in the melan-A cell line was 6.1 μg / ㎖. In addition, as shown in Fig. 4, brown melanin pigment was significantly reduced as the amount of extract added to the extract of black wormwood, and also tyrosinase inhibition rate of 2 mM arbutin (α-arbutin, Sigma, USA) used as a conventional whitening agent in Figure 5 Considering that 62.3%, the inhibitory effect of melanin synthesis due to the tyrosinase inhibitory activity of the extract of black wormwood of the present invention was found to be quite strong.

Cabbage Worm Extract
Treatment concentration (μg / ml) Melanin production inhibitory activity (%) 10 15.9 20 75.3 30 82.4 2 mM arbutin 62.3

Therefore, the extract of the locust mugwort of the present invention shows excellent tyrosinase inhibitory activity in in vitro experiments and can be usefully used as a composition for skin whitening showing excellent melanin synthesis inhibitory activity in experiments with animal cells.

Experimental Example 3. Cytotoxicity Test

In order to determine the cytotoxicity of the extracts of the Japanese mugwort worms, the toxicity was confirmed by MTT assay. Human fibroblasts (fibroblasts) were inoculated in 96-well plates at 8 × 10 ³ cells / well per well and incubated for 24 hours in 200 μl Dulbecco Minimum Essential Medium (DMEM) medium, followed by 0 ~ By treating the extracts of black wormwood by concentration of 0.2% was incubated again for 24 hours. The cultured plate was added with 0.5 mg / ml MTT solution (Sigma, USA), and after 4 hours, the medium was removed, and the cells were lysed with 200 μl of DMSO solution. Absorbance at 570 nm was measured. As the control group, the extract of the locust mugwort worm extract was used as a control, and the cell proliferation rate was calculated according to the following Equation 1, and the results are shown in Table 3 below.

<Equation 1>

% Cell proliferation = (absorbance of experimental group / absorbance of control group) × 100

Cabbage Worm Extract
Treatment Concentration (%) Cell proliferation (%) 0.01 101.1 0.05 101.8 0.10 102.2 0.15 102.6 0.20 103.1 0.25 102.8 0.30 103.2 Control group 100.0

Hereinafter, the formulation of the present invention is exemplified as a cream, massage cream, lotion, skin lotion, essence, packs, cleansing foam formulation, but is not limited to the formulation comprising the cosmetic composition of the present invention.

Formulation Example 1 Cream Composition

In the composition shown in Table 4, the oil phase and the water phase were prepared by heating and mixing the mixture at 75 ° C., respectively.

number Raw material name  Example 1 Comparative Example 1 One Cetostearyl alcohol 1.0 1.0 2 Self-emulsifying glycerin monostearate 1.0 1.0 3 Lipophilic Monostearic Acid Glycerin 2.5 2.5 4 Micro stalin lead 2.0 2.0 5 Methyl paraoxybenzoate 0.2 0.2 6 Paraoxybenzoic acid propyl 0.1 0.1 7 Beeswax 2.0 2.0 8 Monostearic acid polyethylene glycol 2.0 2.0 9 Sorbitan monostearate 1.0 1.0 10 Tri (Caprylic, Caprylic Acid) Glycerin 5.0 5.0 11 Liquid linolin 5.0 5.0 12 Myristic acid octyldodecyl 8.0 8.0 13 Squalane 8.0 8.0 14 Concentrated glycerin 5.0 5.0 15 1,3-butylene glycol 5.0 5.0 16 Allantoin 0.1 0.1 17 Crunchy Worm Extract 10.0 - 18 Spices a very small amount a very small amount 19 Yellow No. 4 a very small amount a very small amount 20 Purified water Balance Balance system 100 100

(Unit: weight%)

Formulation Example 2 Massage Cream Composition

In the composition of Table 5 below, the oil phase and the water phase were each dissolved by heating at 75 ° C., and then cooled to room temperature.

number Raw material name Example 2 One Cetostearyl alcohol 2.0 2 Lipophilic Monostearic Acid Glycerin 2.5 3 Self-emulsifying glycerin monostearate 1.5 4 Methyl paraoxybenzoate 0.2 5 Paraoxybenzoic acid propyl 0.1 6 vaseline 3.5 7 Monostearic acid polyethylene glycol 3.5 8 Sesquioleic acid sorbitan 1.8 9 Liquid paraffin 35.0 10 Tri (Caprylic, Caprylic Acid) Glycerin 3.0 11 Octyldodecanol 5.0 12 Squalene 2.0 13 Concentrated glycerin 5.0 14 Crunchy Worm Extract 5.0 15 Hyaluronic Acid Extract 1.0 16 Spices a very small amount 17 Purified water Balance system 100

(Unit: weight%)

Formulation Example 3 Lotion Composition

In the composition shown in Table 6 below, the oil phase and the water phase were each heated to emulsified at 75 ° C., and then cooled to room temperature.

number Raw material name Example 3 Comparative Example 2 One Cetostearyl alcohol 2.0 2.0 2 Stearic acid 1.0 1.0 3 Lipophilic Monostearic Acid Glycerin 1.0 1.0 4 Self-emulsifying glycerin monostearate 2.0 2.0 5 vaseline 1.0 1.0 6 Monostearic acid polyethylene glycol 1.5 1.5 7 Sesquioleic acid sorbitan 1.0 1.0 8 Liquid paraffin 5.0 5.0 9 Squalene 5.0 5.0 10 Paraoxybenzoic acid propyl 0.2 0.2 11 Methyl paraoxybenzoate 0.2 0.2 12 Concentrated glycerin 5.0 5.0 13 Crunchy Worm Extract 15.0 - 14 Triethanolamine 0.5 0.5 15 Carboxy Vinyl Polymer 0.2 0.2 16 Spices a very small amount a very small amount 17 Purified water Balance Balance system 100 100

(Unit: weight%)

Formulation Example 4. Skin Lotion Composition

In the composition of Table 7 below, the aqueous phase and the ethanol phase were produced and mixed, respectively, and filtered.

number Raw material name Example 4 One glycerin 2.0 2 1,3-butylene glycol 2.0 3 Citric acid 0.01 4 ethanol 15.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 Crunchy Worm Extract 25.0 7 Spices a very small amount 8 Witch Hazel Extract 1.0 9 Purified water Balance system 100

(Unit: weight%)

Formulation Example 5. Essence Composition

In the composition shown in Table 8, the aqueous phase and the ethanol phase were produced and mixed, respectively, and filtered.

number Raw material name Example 5 Comparative Example 3 One Concentrated glycerin 15.0 15.0 2 1,3-butylene glycol 5.0 5.0 3 Allantoin 0.1 0.1 4 ethanol 7.0 7.0 5 Methyl paraoxybenzoate 0.15 0.15 6 Triethanolamine 0.15 0.15 7 Polyoxyethylene Cured Castor Oil 1.0 1.0 8 Tocopherol Acetate 0.5 0.5 9 Carboxy Vinyl Polymer 0.15 0.15 10 Crunchy Worm Extract 30.0 - 11 Spices a very small amount a very small amount 12 Purified water Balance Balance system 100 100

(Unit: weight%)

Formulation Example 6 Pack Composition

In the composition shown in Table 9, the aqueous phase and the ethanol phase were dispersed and dissolved, respectively, and then cooled to room temperature.

number Raw material name Example 6 One Polyvinyl alcohol 15.0 2 Carboxymethylcellulose Sodium 0.3 3 Concentrated glycerin 3.0 4 Allantoin 0.1 5 Ethylenediaminetetradiacetic acid sodium 0.01 6 Polyethylene glycol 1.0 7 ethanol 6.0 8 Methyl paraoxybenzoate 0.15 9 Taraoxybenzoic Acid Profile 0.05 10 Dipanthenol 0.1 11 Polyoxyethylene (12) nonylphenyl ether 0.5 12 Crunchy Worm Extract 45.0 13 Spices a very small amount 14 Purified water Balance system 100

(Unit: weight%)

Formulation Example 7 Cleansing Foam Composition

In the composition shown in Table 10, the aqueous phase and the oil phase were dispersed and dissolved, and then mixed and saponified, and cooled to room temperature.

number Raw material name Example 7 One Stearic acid 6.5 2 Myristic acid 28.0 3 Self-emulsifying glycerin monostearate 3.0 4 Propylene glycol 5.0 5 Concentrated glycerin 10.0 6 Sodium hydroxide 7.0 7 Sodium Ethylenediaminetetraacetate 0.1 8 Placenta extract 0.5 9 Crunchy Worm Extract 15.0 10 Spices a very small amount 11 Purified water Balance system 100

(Unit: weight%)

Experimental Example 4. Confirmation of the whitening effect of the cosmetic composition

The cosmetic compositions prepared in Examples 1, 3, 5 and Comparative Examples 1, 2, and 3 were used for 10 weeks for 30 to 20 females, especially those with black skin, for 10 weeks to observe changes in skin condition. The measurement sites were marked by 1 cm × 1 cm on six places of the arms, and the cosmetic compositions prepared in Examples 1, 3 and 5 and Comparative Examples 1, 2 and 3 were respectively applied. After 10 weeks, skin color (L) was measured using Minolta CR 300, and skin color change (ΔL) was calculated according to Equation 2 below, and the results are shown in Table 11.

<Equation 2>

Skin color change (ΔL) = L before applying cosmetic composition-L after applying cosmetic composition

division Example 1 Example 3 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Control group ΔL 1.53 1.47 1.75 0.25 0.18 0.30 0.00

Experimental Example 5. Confirmation of skin irritation of the cosmetic composition

About 50 healthy adult men and women were patched with about 0.1g of the cosmetic composition prepared in Examples 1, 3, 5, 6 and Comparative Examples 1, 2 and 3 for 24 hours and then removed for 1 hour and 24 hours. The skin condition was visually confirmed and evaluated according to the following criteria, and the results are shown in Table 12 below.

No erythema or unusual phenomenon: ×

Slightly redder than the surroundings: △

Significantly redder than the surroundings: ○

Severe reddening and swelling around: ◎

division Example 1 Example 3 Example 5 Example 6 Comparative Example 1 Comparative Example 2 Comparative Example 3 × 49 48 48 47 48 47 48 △ One 2 2 3 2 3 2 ○ - - - - - - - ◎ - - - - - - -

(Unit: persons)

Figure 1 shows the conversion inhibitory activity according to the concentration (50, 100, 250 μg / ㎖) time by the concentration of the extract of the present invention in the conversion of dopa (DOPA; 3,4-dihydroxyphenylalanine) to dopaquinone (DOPAquinone) Graph showing

2 is a graph showing the tyrosinase inhibitory activity (50, 100, 250 μg / ㎖) according to the concentration of the extract of the black wormwood of the present invention,

3 is a graph showing the melanin pigment formation inhibition rate by the concentration (10, 20, 30 μg / ㎖) treatment of the extracts of the extract of the extract of the mouse melanocytes Melan-A (Melan-a),

Figure 4 is a photograph showing the change of melanin pigment formed by the cell line after the treatment of the concentration of the extract of the extract of the extract of the mouse melanocytes Melan-A (Melan-a), the mouse melanocytes

5 is a graph showing the inhibition of melanin pigment formation by the concentration (0.5, 1, 2 mM) of arbutin (α-arbutin) to the mouse melanocyte cell line Melan-A (Melan-a).

Claims (9)

A cosmetic composition for whitening containing Aster ageratoides Turcz.var.ageratoides extract as an active ingredient. The method of claim 1, Cabbage mugwort extract is a ground part including flowers, branches, stems, leaves, immature fruit, fruit of Cabbage mugwort; Rhizome; And one or more extracts selected from the roots of the cosmetic composition for whitening. The method of claim 1, Cabbage wormwood extract is whitening cosmetic composition, characterized in that extracted with one or more mixed solvents selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, ether and chloroform . The method of claim 1, A whitening cosmetic composition, characterized in that it comprises the extract of S. aeruginosa in 0.001 to 90% by weight. The method of claim 1, A cosmetic composition for whitening is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, oils, waxes, powders, sprays, soaps, cleansing, packs, foundations and hair cosmetics. delete delete delete delete

Images