KR100755427B1 - Cosmetic composition comprising green tea seed oil for enhancing skin elasticity - Google Patents

Abstract

A cosmetic composition for enhancing skin elasticity comprising green tea seed oil is provided to promote growth of fibroblast, collagen biosynthesis and elastin synthesis, remove free radicals, and inhibit oxidation without skin irritation. A cosmetic composition for enhancing skin elasticity comprises 0.001-99.9 wt.%, preferably 1-99 wt.% of green tea seed oil which is prepared by pulverizing seeds of green tea tree(Camellia sinensis), and extracting the pulverized seeds with water, lower alcohol or a mixed solvent thereof at 20-200 deg.C for more than 1 hour through a steam distillation method, and is formulated as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing oil, massage oil, body lotion or body cleanser.

Description

Cosmetic composition containing green tea seed oil having skin elasticity enhancing effect. {Cosmetic Composition comprising green tea seed oil for enhancing skin elasticity.}

The present invention relates to a cosmetic composition containing green tea seed oil as an active ingredient.

In general, the green tea tree ( Camellia sinensis ), also known as tea tree, is a evergreen evergreen tree. Roots extend straight down, 2-4 m deep, 15-20 cm long, with many roots. The leaves vary greatly depending on the variety and location, but they are alternately long, oval-shaped, 6-20 cm long and 3-4 cm wide, with dull teeth at the edges, and pointed at the ends and bottom. Leaves are hard, slightly thick, and glossy on the surface. Flowers bloom in white or light pink in October-November, with 1-3 at the end of leaf axilla or branch. Fruits are capsules, round and gathered, and start to grow in the spring of next year and ripen in autumn, so you can see flowers and fruits at the same time. When the fruit ripens, it bursts, resulting in a hard brown seed. Green tea is picked up and steamed or steamed to inactivate oxidase and dried. These green tea seeds contain caffeine, 22% oil (18% in seeds), and theasaponin (C ₅₇ H ₉₀ O ₂₆ ). Theasaponin is hydrolyzed to theaasapogenol (C ₁₈ H ₃₄ O ₇ ), glucuronic acid (C ₁₈ H ₃₄ O ₇ ), galactose, arabinose, xylose, and angelic acid. Seed also has 33% starch, 8.5% protein. Stems, roots, and seeds contain steroid saponins, especially in seeds (9-10%) (constituents and use of herbs).

In general, human skin loses its elasticity and luster as it ages, becoming keratinized and roughened. Clinically, aging is characterized by wrinkles, reduced skin elasticity, damage and death of skin cells, and systematically the loss of mature collagen and damaged elastin fibers accumulate over time. Such aging of the skin is caused by various factors such as genetic factors, the degree of exposure to ultraviolet rays, the generation of free radicals by physicochemical stress, and the rate and degree of progression are different. Therefore, in order to maintain a young and supple skin, efforts have been made to supply externally, such as nutritional substances that can prevent the aging process.

The stromal protein of the skin exists in the dermal layer and fills the cells, and is composed of collagen, which occupies 90% or more, and elastin, which occupies 3-4%. Skin elasticity can be maintained in the presence of elastic fibers and collagen fibers. Matrix proteins are mainly produced in fibroblasts and decrease by 1% annually as they age. Reduction of matrix proteins reduces the function of each organ. Therefore, the weakening of the skin cells due to the decrease of the matrix protein of the skin causes a decrease in skin elasticity. The reduction of the dermal layer, the most important factor of skin elasticity, can be divided into three factors. First, the number of fibroblasts, which are the main cells of the dermal connective tissue, and the second, are made from fibroblasts, This is because the production of proteins such as collagen and elastin to form is reduced, and the third is because the degradation of proteins in connective tissues already produced is increased. Matrix proteins are degraded by matrix metalloproteinases (MMPs) such as collagenase, elastase, and gelatinase, and their actions are inhibited by tissue inhibitors of metalloproteinases (TIMPs). .

Another cause of skin aging is known as the accumulation of damage caused by reactive oxygen species (ROS) throughout life. In vitro experiments have shown that the antioxidant defense mechanisms within cells decrease in function with aging in liver, brain and heart tissues. There is an antioxidant defense mechanism in the human body to minimize damage caused by ROS. In order to maintain normal cellular function and to suppress aging of the human body, an antioxidant defense mechanism that kills the occurrence of ROS and suppresses damage caused by ROS should exhibit sufficient function.

Therefore, the present inventors conducted a search to develop a matrix protein-promoting substance from plants, and as a result, it was found that the oil component extracted from green tea seed was excellent in synthesizing protein and antioxidant effect of skin cells. Completed.

Looking at the related prior invention, Japanese Patent JP2000095667 uses a green tea leaf and fruit extract to prevent skin roughness, and Japanese Patent JP 2000355545 uses essential oils containing green tea oil to enhance sleep effect. The invention relating to the composition has been filed and registered. However, the above patent is limited to the extraction target of green tea leaves, green tea fruit, etc., there is no application of cosmetics for skin elasticity enhancement using oil extracted from green tea seeds.

An object of the present invention is to provide a cosmetic composition comprising green tea seed oil having a skin elasticity enhancing effect.

In order to achieve the above object, the present invention provides a cosmetic composition containing green tea seed oil having a skin elasticity enhancing effect as an active ingredient.

The green tea seed oil includes a cosmetic composition characterized in that the extraction by steam distillation.

Hereinafter, the present invention will be described in detail.

The green tea seed oil of the present invention is pulverized seeds of Camellia sinensis supplied from Boseong and put into the extractor in water, lower alcohol or a mixed solvent thereof, preferably in distilled water 20 to 200 ℃, preferably 100 ℃ Green tea seed oil of the present invention can be obtained by using an extraction method such as steam distillation, hot water extraction, reflux circulation extraction or pressure extraction for at least 1 hour, preferably at least 6 hours. .

The separated green tea seed oil may be used in an amount of 0.001 to 99.9% by weight, preferably 1 to 99% by weight, based on the total weight of the cosmetic composition.

Green tea seed oil of the present invention can be used in various ways, such as cosmetics and face wash for skin elasticity enhancing effect. The green tea seed oil of the present invention is not particularly limited in its formulation when applied to cosmetics, and specifically, it may be prepared in formulations such as softening cream, nourishing cream, nourishing cream, massage cream, essence and the like.

When the green tea seed oil according to the present invention is applied to the skin, there is less concern about side effects than other synthetic single raw materials, because it is a natural extract, and as a result of the skin toxicity test on the extract, it has been found that there is no effect on the living body It became.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d (l) -alpha tocopherol) and the like. And derivatives thereof (ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, acetic acid dl-alpha tocopherol, nicotinic acid dl-alpha tocopherol, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeast or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, brown seaweed extract, red flush extract, green algae extract, and the like, and carrageenan, arginic acid, sodium arginate and argin purified from these seaweed extracts are preferred. Potassium ginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta sorbitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax and petrolatum.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Coconut nut oil (coconut oil), Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji (牛 脂), Horse oil, Mink oil, Orange rape Animal or vegetable fats and oils, such as oil, jojoba oil, candler wax, carnava wax, liquid lanolin, and hardened castor oil, are mentioned.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As the water-soluble low molecular humectant, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE / POP (Polyoxyethylene / Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Lauric Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallic acid, sorbic acid, and the like.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol and stearyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack Cosmetic composition characterized by having at least one formulation selected from the group consisting of soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tragacanths, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide are used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sacosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  One. Green tea seed  Manufacture of oil

Cut 5 kg of green tea seeds from Boseong and put them in the extractor. The vaporized water vapor is continuously supplied at 100 ° C for 6 hours or more from the lower part of the extractor so that the essential oils are vaporized together with water vapor and passed through a cooling condenser. . In general, essential oils have a lower specific gravity than water, so the separated essential oils float on the water, so that only 41.2 g of green tea seed oil was separated and used as a sample for the following experiment.

Reference Example  One. Green tea seed  Preparation of Extract

After grinding 100g of green tea seeds from Boseong, put 1kg of water or hydrous ethanol and hydrous butylene glycol, extract them at 95 ℃ for 3 hours in an extractor equipped with a cooling condenser, and filter them with 400 mesh filter cloth. After cooling to 7 days at 5-15 ℃ to aging and then filtered by Whatman No. 2 filter paper and 0.45㎛ filter, reduced pressure, concentrated, dried to use as a sample of the following comparative experiment (Experimental Example 5).

Experimental Example  1. Fibroblast proliferation effect

In order to evaluate the effect of green tea seed oil on skin cell proliferation, MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltertazolinum is commonly used to measure the increase and decrease of viable cells in skin fibroblasts. bromide) test. First, cell dilutions were prepared by diluting dermal fibroblasts from human epidermal tissue to 5 × 10 ⁴ cells / ml in DMEM medium containing 10% fetal calf serum.

On the other hand, green tea seed oil was diluted by concentration, 10 ㎕ each and incubated for 24 hours at 37 ℃, 5% CO2 conditions, the culture solution was removed and each well 200 phosphate buffered solution (phosphate buffered saline (PBS) 200 ㎕ After washing twice, 200 μl of MTT (M5655, Sigma) dissolved in DMEM at 1 μl / ml was added to each well and incubated for 2 hours. After removing the culture solution from each well of the 96-well plate, 150 μl of DMSO (Dimethyl Sulfoxide) solution was added to each well, followed by shaking to dissolve formazan deposited in the cells. Then, the cell proliferation effect of green tea seed oil was evaluated by measuring the absorbance at 552 nm with an ELISA leader for the solution present in each well. DMSO (Dimethyl Sulfoxide) was used as a control, and the above procedure was performed to obtain a cell proliferation effect from Equation 1 below and the results are shown in Table 1 below.

 Cell Proliferation Effect of Green Tea Seed Oil Green Tea Seed Oil Treatment Concentration (%) Cell proliferation effect (%) 0.008 103 0.016 106 0.032 109 0.063 115 0.125 117

As shown in Table 1, it can be seen that green tea seed oil has an excellent cell proliferation effect in the proliferation of fibroblasts, which are important for skin elasticity. The proliferation of fibroblasts is closely related to the increased biosynthesis of collagen, elastin, integrin and laminin, which are important for skin wrinkle and skin elasticity.

Experimental Example  2. Collagen biosynthesis promoting effect

In order to examine the effect of green tea seed oil on the skin, the effect of promoting collagen biosynthesis was examined by an in vitro experimental method. The collagen synthesis amount was measured by PIP (Procollagen Type I C-peptide) EIA assay, and quantitatively measured the type I procollagen C terminal peptide (PICP) which is synthesized the most.

1 × 10 ⁴ cells were dispensed into 24 well plates and incubated for 24 hours. After incubation, the medium was replaced with a serum-free medium, and each sample was added at a concentration of 50 µg / ml and incubated in a 37 ° C., 5% CO ₂ incubator for 3 days. As a control, each well was treated with vitamin C, which is known to promote collagen synthesis, at a concentration of 50 μg / ml in each well. The culture supernatant medium was centrifuged and used in a kit (PIP EIA KIT). In a 96 well plate coated with mouse monoclonal antibody against PIP, the antibody-POD conjugate (MK1O1, TAKARA BIO INC.) Labeled with peroxidase was added and reacted thereto. Added.

37 ℃, 5% CO ₂ After 3 hours of reaction in an incubator, 100 μl / well of the substrate solution phenylenediamine was added and developed. After standing at room temperature for 15 minutes, the reaction was stopped by adding 100 µl / well of the stop solution. Absorbance was measured at 450 nm within 5 minutes, and the collagen amount was measured by plotting the PICP standard concentration, and the results are shown in Table 2 below.

Collagen biosynthesis promoting effect Test substance Treatment concentration (%) Collagen Synthesis (ng / ml) Control group (vitamin C) 0.050 325 Green Tea Seed Oil 0.008 244 Green Tea Seed Oil 0.016 253 Green Tea Seed Oil 0.032 289 Green Tea Seed Oil 0.063 331 Green Tea Seed Oil 0.125 392

Collagen synthesis amount of vitamin C as a control was 325 ng / ml, 0.063% of the green tea seed oil of the present invention showed a similar amount of collagen synthesis, 0.125% showed an excellent high collagen synthesis amount.

Experimental Example  3. Elastin ( Elastin A) synthetic effect

After culturing normal human fibroblasts in 24-well microplates for 24 hours, the samples were treated by concentration and incubated for 2 days, and the amount of elastin newly synthesized by fibroblasts was measured using an elastin kit (fastin elastin assay kit). The amount of elastin was corrected by cell number or protein amount, and the results are shown in Table 3 below. Experiments using vitamin C as a control showed similar elastin synthesis effects at 0.125% concentration.

Elastin Synthesis Effect Test substance Treatment concentration (%) Elastin Synthesis (ng / ml ) Control group (vitamin C) 0.050 256 Green Tea Seed Oil 0.008 221 Green Tea Seed Oil 0.016 232 Green Tea Seed Oil 0.032 235 Green Tea Seed Oil 0.063 246 Green Tea Seed Oil 0.125 259

Experimental Example  4. Free radical  Elimination effect

The free radical scavenging effect was tested using the green tea seed oil, vitamin C, alpha-rutin (α-Rutin) and BHT (butylated hydroxy toluene) as positive controls.

1 ml of 0.1 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) (sigma, USA) solution dissolved in methanol was added to a test tube, and 1 ml of ethanol was added to 10 µg of each test sample. The reaction was carried out in a water bath for 30 minutes. At this time, 1 ml of ethanol containing no test substance was added to the negative control group. After the reaction, the absorbance was measured at a wavelength of 516 nm with a spectrophotometery to calculate the free radical scavenging rate of each sample, and the results are shown in Table 4 below. Experiments with substances known to have antioxidant effects as positive controls showed similar effects to other controls at concentrations of 0.125% green tea seed oil.

Free radical scavenging effect division Concentration (%) Free radical scavenging rate (%) Vitamin c 0.05 98.2 Alpha-routine 0.05 93.7 BHT 0.005 97.9 Green Tea Seed Oil 0.063 84.5 Green Tea Seed Oil 0.125 95.7

Experimental Example  5. Green tea seed  Oil and Green tea seed  Of extract Free radical  Elimination comparison experiment

In order to compare the free radical scavenging ability of the green tea seed oil and the green tea seed extract, the free radical scavenging experiment was performed in the same manner as in Experiment 4 using the green tea seed oil of Example 1 and the green tea seed extract of Reference Example 1.

As a result, as shown in Table 5, it was confirmed that the green tea seed oil has more excellent free radical scavenging ability than the green tea seed extracts.

Free radical scavenging effect comparison division Concentration (%) Free radical scavenging rate (%) Green Tea Seed Oil 0.063 84.5 Green Tea Seed Oil 0.125 95.7 Green Tea Seed Water Extract 0.125 57.9 Green Tea Seed Function Ethanol Extract 0.125 77.6 Green Tea Seed Functional Butylene Glycol Extract 0.125 65.9

Formulation examples of the cosmetic composition of the present invention will be described, but the present invention is not intended to limit this, but is intended to explain in detail.

Formulation example  1. Cream

ingredient weight% Purified water To 100 Squalane 15.0 glycerin 3.0 Beeswax 3.0 Propylene glycol or butylene glycol 7.0 Cetostearyl alcohol 2.5 Lipophilic monostearic acid stearate 2.0 Monostearic acid polyoxyethylene (20) sorbitan 1.5 Stearic acid 1.5 Sorbitan sesquioleate 0.5 Methylpolysiloxane 0.3 Spices 0.2 Arginine 0.1 Carbomer 0.1 Sodium Ethylenediaminetetraacetate 0.02 Green Tea Seed Oil 1.0

Formulation example  2. Essence

ingredient weight% Purified water To 100 Betaine 8.0 Hydroxyethyl Cellulose 0.2 glycerin 7.0 ethanol 5.0 Seaweed Extract 0.5 Gold and Silver Extract 0.5 Dipanthenol 0.3 Polyoxyethylene Cured Castor Oil 0.3 Allantoin 0.2 Carboxy Vinyl Polymer 0.13 Spices 0.1 Arginine 0.13 Benzophenone-5 0.02 Ethylenediamine Sodium Acetate 0.02 Green Tea Seed Oil 0.5

Formulation example  3. skin  Lotion

ingredient weight% Purified water To 100 ethanol 7.0 glycerin 3.0 Betaine 2.0 Polyoxyethylene Cured Castor Oil 0.5 Phenyldimethicone 0.5 Spices 0.1 Carboxy Vinyl Polymer 0.05 Arginine 0.05 Sodium Ethylenediaminetetraacetate 0.02 Benzophenone-5 0.02 Green Tea Seed Oil 0.1

Formulation example  4. Massage Cream

ingredient weight (%) Purified water To 100 Green Tea Seed Oil 20.00 Hydropolyisobutene 10.00 Butylene Glycol 7.00 glycerin 3.00 Ethylhexyl isononate 2.00 Glyceryl Stearate / Fiji 100 Stearate 1.20 Macadamia Nut Oil 1.00 Cetyl alcohol 2.00 Sodium Acrylate / Acroyldimethyltaurate Copolymer / Isohexadecane / Polysorbate 80 0.30 Parabens 0.30 Tocopheryl Acetate 0.20 Carbomer 0.10 Arginine 0.10

Formulation example  5. Massage oil

ingredient weight (%) Green Tea Seed Oil 96.80 Tocopheryl Acetate 3.00 Spices 0.20

As described above, the present invention relates to a cosmetic composition containing green tea seed oil having fibroblast proliferation effect, collagen biosynthesis promoting effect, elastin synthesis promoting effect, and free radical scavenging effect. It can be usefully used as a cosmetic composition effective to enhance skin elasticity.

Claims (3)

Cosmetic composition containing green tea seed oil having skin elasticity effect as an active ingredient. The cosmetic composition according to claim 1, wherein the green tea seed oil is extracted by steam distillation. The composition of claim 1, wherein the composition comprises skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, Cosmetic composition, characterized in that it has at least one formulation selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, cleansing oil, massage oil, body lotion and body cleanser.