KR20060008570A - Composition comprising the seed oil of green tea having cholesterol-lowering or antioxidant activity - Google Patents

Abstract

The present invention relates to a composition containing green tea seed oil having cholesterol lowering or antioxidant activity as an active ingredient. Specifically, the green tea seed oil of the present invention lowers plasma cholesterol levels and significantly reduces the degree of lipid peroxidation in the liver. Since the high fat diet is effective to protect against oxidative stress caused by ingestion, the composition comprising the same may be useful as a pharmaceutical composition and health functional food for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress. have.

Green tea seed oil, lipid peroxidation, cholesterol lowering, antioxidant, pharmaceutical composition, health functional food

Description

Composition comprising the seed oil of Green Tea having Cholesterol-lowering or antioxidant activity}             

1 is a diagram showing the weight gain of C57BL / 6 mice bred for 9 weeks with each experimental diet.

Figure 2 is a diagram showing the results of measuring cholesterol changes from the eye blood every three weeks,

Figure 3 is a diagram showing the results of measuring the degree of oxidative stress of liver tissue TBARS.

The present invention relates to a composition containing green tea seed oil having cholesterol lowering or antioxidant activity as an active ingredient.

Antioxidation refers to the prevention of various oxidations in the body. Oxidation refers to a phenomenon in which various kinds of unsaturated fatty acids present in the body are oxidized by oxygen to produce a substance called lipid peroxide, which increases aging. O ₂ occurring in human disease, and aging the body metabolic processes ^{- (superoxide), NO · (} nitric oxide), NO 2 · (nitrogen dioxide), OH · (hydroxyl), ROO · (proxyl), RO · (alkoxyl ), HO ₂ · (hydroperoxyl) radicals (radical), etc., and in vivo as a means to defend the body from these free radicals that are harmful radicals, superoxide dismutase (SOD), catalase (CAT antioxidants such as catalase) and glutathione peroxidase (GSHpx) and antioxidants such as vitamin E and glutathione to protect the body from oxidative damage by removing free radicals (Tiidus PM. et al, Sports Med, 20 , pp 12-13, 1995). However, when these antioxidant defenses are too low to control the production of free radicals, oxidative stress and damage of tissues are promoted. Excess free radicals and lipid peroxides produced in the body are injured by oxidative stress such as protein oxidation and DNA damage. (Kumar CT, et al, Mol. Cell Biochem., 111, pp 109-115, 1992). Therefore, many synthetic or natural antioxidants have been developed to reduce this oxidative stress, but synthetic antioxidants have safety problems (Branen, AL, J. Am. Oil. Chem. Soc., 55 , 123-126). , 1975; Ito, N., et al, J. Natl. Cancer Inst., 70 , pp343-349, 1983), and recently, to develop natural antioxidants that are safer and more effective in various herbal and edible plant extracts. (Lim, DK, et al, Korean J. Food Sci. Technol. , 28 , pp83-89, 1996; Kim, HK, et al, Korean J. Food Sci.Technol . , 27 , pp80- 85, 1995; Cha, JY, J. Korean Soc, Food Nutr. , 28 , pp1131-1136, 1999; Kim, MH, et al, Korean J. Food Sci. Technol ., 31 , pp 273-279, 1999).

Antioxidants present in plant foods are typical of carotenoids, flavonoids, and polyphenols, along with vitamins A, C, and E.

Green tea of the present invention is one of foods that have recently been of interest due to its excellent antioxidant power among natural functional substances, and contains a large number of ingredients including catechin, which is a polyphenolic compound. It is known to have excellent function of eliminating free radicals caused by stress in vivo and externally (Kim MJ, et al, Nutrition Research, 22 , pp733-744, 2002; Rhee SJ, et al, Kor. J. Gerontol., 8 , pp 49-55, 1998). Studies on the antioxidant activity of green tea include Nakayama's research (Nakayama T., University of Shizuoka, Japan, 7 , pp1991-1993, 1994) .The polyphenolic structures of catechins were converted to hydrogen peroxide (H ₂ O _2). And cytotoxicity, and flavonoids were effective in removing singlet oxygen and free radicals in the early stages of lipid peroxidation. In addition, functional components related to geriatric disease prevention or cancer prevention (Fujita, Y., et al, Jpn, J. Cancer Ues ., 80 , p503, 1989), suppression of growth of acquired immunodeficiency virus (Wilson, IA and Cox, NJ, Ann. Uev. Immunol. , 8 , p737, 1990), tooth decay (Hattori, et al, Chem. Pharm. Bull. , 38 , p717, 1990), cholesterol inhibitory activity (Muramatsu, K., et al, J. Nutr. Sci. Vitaminol., 32 , p613, 1986), anti-aging (Masak, H., et al, Nippon Nogeikagaku Kaishi. , 59 , p129, 1985) and antibacterial agents (Hara, Y., et al, Nippon Shokuhin Kogyo Gakkaishi., 36 , p951, 1989).

Fats and oils, along with carbohydrates and proteins, are not only concentrated energy sources, but also important components for living organisms, including cell membranes, protecting the subcutaneous tissue, organs, and insulators of the lacrimal nerves. . Oils have traditionally been derived from plants, animals, and fish, and are the main constituents of shortening, margarine, salad and cooking oils. Excessive fat intake causes health problems such as heart disease, obesity and cancer. Therefore, while lowering the calorie content of fats and oils to reduce the need to save the unique functionality. In other words, while reducing the health problems caused by the consumption of fats and oils, while maintaining the palatability of fats and oils, the taste and melting in the mouth, while maintaining the intake of essential fatty acids essential to the human body should be explored. In order to improve the intake of dietary supplements, we need to produce and develop a new concept of fats and oils consisting only of essential ingredients with functional ingredients. Development of new maintenance materials is urgently needed.

The fatty acid composition of green tea seed oil has a mixed oil pattern of corn oil, which is an excellent cooking oil of Korea, and olive oil, which is the highest-priced oil, which is imported from Korea, but there is little research. In addition, while it is rarely used in Korea, it is already used in various fields such as cosmetics and medical products as expensive high-quality cooking oil and functional products. In light of the fact that Korea is replacing the high demand of fats and oils with the import of expensive olive oil, green tea seed oil is considered as an excellent oil material that can replace olive oil. Mayonnaise, various sources and beauty materials have high utilization value, and it is urgent to develop because it has the advantage of extending the shelf life by enhancing the oxidation stability of oils and fats.

Therefore, as a previous study, the present inventors have tested the effect on the antioxidant detoxification function of liver tissue, which is the central organ of all metabolism, against the green tea seed oil whose fatty acid composition is similar to olive oil based on the functionality of green tea, The present invention was completed by confirming that the high fat diet had an effect of protecting against oxidative stress caused by ingestion.

An object of the present invention to provide a composition for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress containing green tea seed oil having cholesterol lowering or antioxidant activity as an active ingredient and a pharmaceutically acceptable carrier, diluent or excipient It is.

In order to achieve the above object, the present invention provides a composition for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress, including green tea seed oil having cholesterol lowering or antioxidant activity.

Specifically, the present invention provides a pharmaceutical composition for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress, including green tea seed oil having cholesterol lowering or antioxidant activity and including a pharmaceutically acceptable carrier, diluent or excipient. do.

The present invention also relates to hyperlipidemia or oxidative stress, which includes green tea seed oil having cholesterol lowering or antioxidant activity that is separable from the nonpolar or polar solvent soluble extract of green tea seed, and that includes a pharmaceutically acceptable carrier, diluent or excipient. It provides a pharmaceutical composition for the prevention and treatment of diseases caused.

The nonpolar solvent soluble extract comprises an extract soluble in a nonpolar solvent, preferably hexane, selected from hexane, ethyl acetate or chloroform.

Hereinafter, the present invention will be described in detail.

The green tea seed oil of the present invention is powdered by removing the outer skin of the green tea seed, and then about 1 to 20 times the weight of the green tea seed powder, preferably about 1 to 10 times the volume of water, and a lower alcohol having 1 to 4 carbon atoms. Or polar solvents such as mixed solvents having a mixing ratio of about 1: 0.1 to 1:10 or nonpolar solvents such as hexane, ethyl acetate, chloroform, and preferably nonpolar solvents such as hexane, ethyl acetate, chloroform, and the like. Preferably, the extraction method is repeated 1 to 5 times, preferably 2 to 3 times, using hexane for about 12 hours to 2 days, preferably about 12 hours to 1 day. Is obtained and then purified and concentrated.

The present invention provides a pharmaceutical composition containing green tea seed oil which is separable from the non-polar solvent soluble extract of green tea seed obtained according to the preparation method as an active ingredient.

 In addition, the present invention comprises a green tea seed oil having a cholesterol lowering or antioxidant activity obtained through the extraction separation process as described above from a non-polar solvent soluble extract or a polar solvent soluble extract of green tea seed and a pharmaceutically acceptable carrier, diluent or It provides a pharmaceutical composition for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress comprising an excipient.

The composition of the present invention comprises from 0.1 to 50% by weight of green tea seed oil obtained from the extract relative to the total weight of the composition.

Diseases caused by hyperlipidemia or oxidative stress include adult diseases such as aging, hypertension, arteriosclerosis, stroke, fatty liver, cirrhosis, diabetes, cataracts, acquired immunodeficiency, chronic bronchitis, emphysema and malignant tumors, and various cancers. The compositions of the invention are effective in the prevention and treatment of aging or adult diseases with cholesterol lowering and antioxidant efficacy.

The composition comprising green tea seed oil of the present invention may further comprise a suitable carrier, excipient or diluent according to conventional methods.

Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The composition comprising the green tea seed oil obtained from the extract according to the present invention, respectively, oral formulations, external preparations, suppositories or sterile injections of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols etc. according to conventional methods It can be formulated and used in the form of a solution.

Specifically, when formulated, it may be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, and the like. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like. Such solid preparations include at least one excipient such as starch, calcium carbonate, It may be prepared by mixing sucrose, lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc can also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups, and include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. Can be. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Green tea seed oil obtained from the extract of the present invention may vary depending on the age, sex, and weight of the patient, but generally in an amount of 0.01 to 500 mg / kg, preferably in an amount of 0.1 to 100 mg / kg once or several times a day. It can be administered separately. In addition, the dosage of the green tea seed oil obtained from the extract may be increased or decreased depending on the route of administration, the degree of disease, sex, weight, age and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, humans, etc. by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

Green tea seed oil of the present invention has little toxicity and side effects, and thus can be used safely even when taken for long periods of time.

The present invention also provides a health functional food for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress comprising the green tea seed oil and a food supplement acceptable food supplement.

The composition containing the green tea seed oil of the present invention may be used in a variety of drugs, foods and beverages for the prevention of diseases caused by hyperlipidemia or oxidative stress. Foods to which the green tea seed oil obtained from the extract of the present invention can be added include various foods, for example, beverages, gums, teas, vitamin complexes, health supplements, and the like. , Capsules or beverages.

At this time, the amount of green tea seed oil obtained from the extract in the food or beverage can be generally added to 0.01 to 15% by weight of the total food weight in the case of the health food composition of the present invention, 0.02 based on 100ml for the health beverage composition To 10 g, preferably 0.3 to 1 g.

The health beverage composition of the present invention has no special limitation except for containing green tea seed oil obtained from the extract as an essential ingredient in the indicated ratio, and contains various flavors or natural carbohydrates as additional ingredients, as in general beverages. can do. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; Polysaccharides such as dextrin, cyclodextrin; Conventional sugars such as and the like and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. have. The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and the like. Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.

Example 1 Preparation of Green Tea Seed Oil

Green tea seeds were purchased from Hadong Hwagae Jedda and the outer husks of green tea seeds were removed and then powdered. Hexane (hexane) corresponding to 10 times of 1 kg of green tea seed powder sample was added thereto, extracted at room temperature for 24 hours, and concentrated to obtain 301.3 g of green tea seed oil (yield 30.13%).

As a result of the green tea seed oil and the comparative control group of the present invention, the components of the fatty acid composition of olive oil and corn oil were commissioned by Unifood Tech Co., Ltd., a national test and testing institution (Food Safety Agency Certification No. 20). The results of component analysis are shown in Table 1 below.

Fatty acid Corn oil Green Tea Seed Oil olive oil C16: 0 5.7 16.8 10.1 C18: 0 1.7 1.4 3.2 C20: 0 0.4 One 0.5 C21: 0 0.06 ND ND C22: 0 ND ND ND C16: 1, cis-9 ND ND 0.6 C18: 1, cis-9 23 53.9 78.7 C18: 1, trans-9 0.8 ND 6.4 C18: 2, cis-9, 12 61.4 26.9 ND C18: 2, trans-9,12 0.4 ND ND C18: 3, cis-9, 12, 15 6 ND 0.5 ND: not detected

As shown in Table 1, the fatty acid composition of green tea seed oil had the highest content of oleic acid as 53.9%, and linolenic acid as 26.9%. That is, the fatty acid composition of green tea seed oil showed a polyunsaturated fatty acid content less than that of corn oil and somewhat higher than that of olive oil, indicating a mixed oil of corn oil and olive oil.

Experimental Example 1 Breeding and Composition of Dietary Animals

1-1. Breeding of Experimental Animals

The experimental animals were purchased from Hyochang Science (Seoul, South Korea) and 5 weeks old C57BL / 6 mice weighing 20.1 ± 0.24g and pre-raised for the first week using a stock diet. According to the numbers, three animals were placed in a plastic cage by a completely randomized design, and 12 animals of each group were divided into three groups of corn oil, green tea seed oil, and olive oil for 9 weeks. Blood was taken from the eye every three weeks and cholesterol changes were observed. The diet and water were fed by the ad libitum feeding method, and 4% thioglycolate (TGC) in the abdominal cavity for activation of macrophages two days before sacrifice. sacrificed after injection of thioglycollate. During the breeding period, ground water was supplied to the drinking water, and the temperature of the breeding room was maintained at 22-25 ° C., and the contrast was turned on and off every 12 hours. Dietary intake was measured twice a week and body weight once a week. Dietary efficiency (%) was calculated by Equation 1 below, and the measured values were expressed as mean ± SEM, respectively (P <0.05: Duncan's multi-range test).

Dietary Efficiency (%) = Weight Gain (g) / Dietary Intake (g) × 100

As a result, the weight change of the experimental group was the highest in the olive oil group and there was no difference between corn oil and green tea seed oil (see Fig. 1). The results of weight gain, dietary intake, and dietary efficiency are shown in Table 2 below. The dietary intake was significantly lower in the green tea seed oil group ( p <0.05), but there was no significant difference in dietary efficiency between groups. In other words, intake of green tea seed oil showed the same effect as the corn oil group, and liver weight was similar to corn oil group.

Corn oil Green Tea Seed Oil olive oil Weight gain (g / day) 0.376 ± 0.007 0.375 ± 0.006 0.395 ± 0.005 Dietary Intake (g / day) 3.834 ± 0.18 2.839 ± 0.159 3.252 ± 0.037 Dietary efficiency (%) 9.80 ± 0.97 13.21 ± 0.97 12.15 ± 0.83 Weight of liver (g) 1.936 ± 0.049 1.955 ± 0.046 1.675 ± 0.065 NS: not significant

1-2. Composition of the diet

The diet used in this experiment was an atherosclerosis diet to facilitate atherosclerosis in C57BL / 6 mice, and corn oil, green tea seed oil, and olive oil were added to the diet to achieve the same level of energy. A semipurified diet was created. Green tea seed oil, olive oil and corn oil each 10% for 100g of feed was mixed with 5% corn oil and 1% cholesterol in each diet, the composition of the diet is shown in Table 3 below. Each diet was fed to experimental animals while frozen.

Mineral mixture, vitamin mixture, DL-methionine, Choline bitratrate, sodium cholate were used as ICN products, and casein was used as corn starch corn products. starch) is Doosan Corporation, sucrose is Samyang Sugar's refined sugar product, cocoa butter is Lotte Samgang product, corn oil is commercially available Ottogi Corp. (Seoul, Korea), and olive oil is American Extra verzin was purchased and used.

ingredient Corn oil group Green Tea Seed Group Olive oil group Casein 20 20 20 Sucrose 25 25 25 Corn starch 28.5 28.5 28.5 Fat 15 15 15 Corn oil 15 5 5 Green tea seed oil 10 Olive oil 10 Cholesterol 1.25 1.25 1.25 DL-methionine 0.3 0.3 0.3 Vitamine One One One Mineral 3.5 3.5 3.5 Cellulose 5 5 5 Sodium cholate 0.5 0.5 0.5 Cholin bitartrate 0.2 0.2 0.2 Sum 428cal

1-3. Sample Collection and Treatment of Laboratory Animals

4% thioglycolate was injected into the abdominal cavity 2 days before sacrifice for 9 weeks and sacrificed for 48 hours after 48 hours, followed by anesthesia using ether respiratory anesthesia, and then treated with heparin from the eye. The blood was taken using the capillary tube. Soy sauce was extracted, washed with PBS (phosphate buffer saline, pH 7.4), dried with a filter paper, weighed, soaked in liquid nitrogen, and stored at -70 ° C.

Experimental Example 2 Measurement of Cholesterol Cholesterol

Blood was taken from the eye every three weeks to quantify cholesterol. The total cholesterol content of the plasma was measured by the kit reagent for quantification by the enzyme method (Yongdong Pharmaceutical). 0.75 ml of the reaction reagent mixed with the prepared enzyme solution was added to 5 µl of each sample, followed by mixing for 5 minutes in a 37 ° C water-bath. Blanks replaced with distilled water instead of samples were measured using a spectrophotometer as a control. The standard curve was used by diluting 300 mg / dl of cholesterol standard solution by concentration.

As a result, the plasma cholesterol concentration was increased in all groups until 6 weeks of dietary intake, but the increase level was found to be significantly lower than the green tea seed oil group compared to the existing group (see Fig. 2).

Experimental Example 3 Measurement of Lipid Peroxidation in Liver Tissue

The lipid peroxide of the liver was measured by slightly modifying the method of Okawa et al. (Okawa H., et al., Analytical. Biochem. , 95 , pp351, 1979).

In other words, 0.1 g of liver was homogenized with 1 ml of 50 mM HEPES buffer containing 125 mM KCl, and then 150 µl of sample and 50 µl H ₂ O mixed solution were incubated at 37 ° C. for 60 minutes, and then 0.4% TBA, 15% TCA and 2.5% 400 μl of the TBARS solution mixed with HCl was added thereto, followed by reaction in a 95 ° C. water bath for 60 minutes, and then cooled in cold water and centrifuged at 4,000 rpm for 10 minutes. Take the separated supernatant and measure the absorbance at 540 nm, using standard 1,1,3,3-tetramethylpropane (TMP; 1,1,3,3-tetramethoxypropane) as standard Substituted in the curve was converted to the amount of malondialdehyde (MDA; malondialdehyde).

As a result, it was confirmed that the oxidative stress of liver tissue was significantly suppressed in the green tea seed oil group and the olive oil group as compared to the control group corn oil group (see FIG. 3).

It describes an example of the formulation of the pharmaceutical composition comprising the green tea seed oil of the present invention, the present invention is not intended to limit it, it is intended to explain in detail only.

Formulation Example 1 Preparation of Powder

Green Tea Seed Oil 300 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Green Tea Seed Oil 50 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

Green Tea Seed Oil 50 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

Green Tea Seed Oil 50 mg

Appropriate sterile distilled water for injection

pH adjuster

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Green Tea Seed Oil 100 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

According to the conventional method for preparing a liquid, each component is added and dissolved in purified water, lemon flavor is added to the mixture, and then the above ingredients are mixed, purified water is added, the total is adjusted to 100 ml by adding purified water, and then filled into a brown bottle. Sterilized to prepare a liquid.

Formulation Example 6 Preparation of Health Food

Green Tea Seed Oil 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 7 Preparation of Health Beverage

Green Tea Seed Oil 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilized and then refrigerated It is used to prepare a healthy beverage composition of the present invention.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

As described above, since the composition containing the green tea seed oil of the present invention as an active ingredient exhibits cholesterol lowering and antioxidant effects, a pharmaceutical composition for preventing and treating aging, various adult diseases or cancers due to hyperlipidemia or oxidative stress, etc. And as a dietary supplement.

Claims (8)

A pharmaceutical composition for the prevention and treatment of diseases caused by hyperlipidemia or oxidative stress, comprising green tea seed oil having cholesterol lowering or antioxidant activity and comprising a pharmaceutically acceptable carrier, diluent or excipient. The pharmaceutical composition of claim 1, wherein the green tea seed oil is separable from a nonpolar solvent or a polar solvent soluble extract of green tea seed. The pharmaceutical composition of claim 2, wherein the nonpolar solvent soluble extract is extracted with hexane, ethyl acetate or chloroform. The pharmaceutical composition of claim 3, wherein the nonpolar solvent is hexane. According to claim 1, wherein the disease caused by hyperlipidemia or oxidative stress is atherosclerosis, including aging, hypertension, cerebral infarction, angina pectoris and myocardial infarction, adult diseases such as stroke, fatty liver, cirrhosis, diabetes and cataracts, acquired immunodeficiency, chronic A pharmaceutical composition that is bronchitis or cancer disease such as emphysema and malignancy. The composition according to any one of claims 1 to 5, wherein the composition is formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols such as aerosols, external preparations, suppositories or sterile injectable solutions. Pharmaceutical composition, characterized in that. A dietary supplement comprising green tea seed oil having a cholesterol lowering or antioxidant activity and a food acceptable additive. The dietary supplement of claim 6 in the form of a pill, powder, granule, acupuncture, tablet, capsule or beverage.

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