KR20150085215A - Composition comprising black garlic and Gastrodia elata extract with antioxidant activity and method of making the same - Google Patents

Abstract

The present invention discloses an antioxidant composition including black garlic and a Gastrodia extract and a manufacturing method thereof. The black garlic and the Gastrodia extract of the present invention are enhanced with a physiologically active component having an antioxidant function due to the mixed composition of the black garlic and Gastrodia, and may be effectively used for treating diseases requiring an antioxidant function, various food additives or health functional food.

Description

[0001] The present invention relates to an antioxidative composition comprising black garlic and a chewy gum extract, and a method for producing the antioxidant composition.

The present invention is in the field of natural extracts, specifically black garlic and chymus extracts.

Reactive oxygen species (ROS) are one of the major causes of aging. The active oxygen species are generated by the energy metabolism process and the immune reaction, and are also caused by the external harmful environment.

Active oxygen is very reactive and causes DNA denaturation, excessive signal transduction and protein denaturation in the body. These deleterious reactions are inhibited by antioxidants or antioxidant enzymes present in vivo and are intended to maintain homeostasis. However, the aging of the antioxidant system and the accumulation of reactive oxygen species by continuous noxious stimulation may aggravate the homeostasis, promote aging, and cause various diseases such as skin diseases, skin cancer, arteriosclerosis and thrombosis (Laure Rittie et al. , Aging Research Reviews, 1, 705-720, 2002; Cutler RG, Annals of the New York Academy of Sciences, 1055, 93-135, 2005).

Synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are widely used in commercial foods because they have excellent antioxidant power and low cost, but their use is regulated due to safety concerns such as human side effects. Natural antioxidants, such as tocopherol, are safe for the human body, but they have the disadvantage that they are low in oxidative chain reaction inhibiting ability and expensive.

Currently, there is a growing interest in antioxidants that inhibit the formation of active oxygen systems or remove the active oxygen system formed. Especially, as wellness and health become social issues, studies on various natural extracts have been conducted recently, and the demand for functional foods using natural extracts is steadily increasing.

Korean Patent Laid-Open Publication No. 2011-0130132 relates to an antioxidant active composition containing an extract of an acid and a white flower, and discloses an antioxidant composition used for a cosmetic composition or the like.

Korean Patent Laid-Open Publication No. 2013-0070683 relates to a composition containing an extract of Aloe vera extract having an antioxidative activity, and discloses a food composition and a cosmetic composition for antioxidation.

However, the development of antioxidants which are still safe and effective from natural materials is in urgent need, and the market demand for the development of excellent materials having new action mechanism from various medicinal plants and agricultural life resources is very high.

The present invention provides an extract, a pharmaceutical composition and a health functional food which can be effectively used for antioxidation using a natural substance.

In one embodiment, the present invention provides an antioxidant composition comprising black garlic and chymus extract as an active ingredient.

The compositions according to the present invention comprise a combination of black garlic and chymus extract, wherein the active ingredient, in particular the content of erythro- thionine, is maximized as compared to each alone, in one embodiment about 10: 1 by weight of black garlic extract do.

The black garlic and chymus extracts included in the composition according to the present invention are extracted by hot water extraction in one embodiment.

The composition according to the present invention maximizes the content of ergothioneine by the combination of black garlic and chymus extract. Ergotothione is a representative antioxidant, and thus the composition according to the present invention can be usefully used for the prevention or treatment of diseases which are effective due to antioxidant action.

In another aspect, the invention also provides an antioxidant food composition comprising black garlic and chymus extract, which may be provided as a health supplement, functional food, beverage or food additive and may be used as an antioxidant food composition or as an improvement ≪ / RTI >

In another aspect, the invention also provides a method of producing black and ginseng extracts, comprising mixing black garlic and chewing gum; Hydrothermally extracting the mixture; And filtering and concentrating the extract. The present invention also provides a method for producing black garlic and chymus extract. In one embodiment, the present hot water extraction can be performed at about 70 ° C to 100 ° C for about 1 to 3 hours. In one embodiment, the black garlic and the chimney may be mixed in a weight ratio of about 10: 1. In one embodiment, the method further comprises stirring in the hot water extraction step.

In another aspect, the present invention provides a method for producing black garlic and chymus extract, wherein antioxidant components are enhanced by mixing a black horse with a horse chestnut, and extracting the black horse with a mixture of black horse and horse chestnut.

In one embodiment according to the present invention, the black garlic and the chimney are mixed in a weight ratio of 10: 1. In another embodiment, the black garlic and / or chymus extract in the method according to the invention is hot-water extracted and the hot water extraction can be carried out at about 70 ° C to 100 ° C for 1 to 3 hours.

The composition containing the black garlic and chymus extract of the present invention and the health food containing the same have various antioxidative effects that show a strong antioxidative effect due to increased ergotioneine content. Treatment, prevention, amelioration and / or alleviation.

The present invention relates to an antioxidant food composition containing black garlic and chymus extract as an active ingredient, and is based on the finding that antioxidant components as a useful ingredient are remarkably improved by using a combination of black garlic and horse chestnut.

In the present application, garlic ( Allium sativum L. ) is a perennial bulbous plant that is used as a food and belongs to ali of lilies together with onion, onion, leek and soya. The major components are alliin, allicin, and disulphide. Alicin is known to have antimicrobial and detoxifying effects (M. Arzanlou, et al., Toxicon , 57, 540- 545, 2010). One embodiment of the present invention is garlic, which is crude. In another embodiment herein garlic is processed garlic, especially black garlic. In another embodiment of the present application, the black garlic is a black garlic made of crude or garlic. Black garlic can be prepared by aging raw garlic for a certain period of time under appropriate temperature and humidity conditions. Methods for producing such black garlic are known, and for example, The physiological activity and antioxidative effect of the black garlic extract prepared by aging, Jang Eun Kyung, etc., the Korean Food Science Journal, 40 (4), 443-448, 2008) can be used or can be purchased commercially Agricultural, Korea). A black garlic extract is used in the composition of the present invention. In the present invention, the black garlic extract comprises an active ingredient isolated from a natural product, that is, a substance showing a desired activity, and is usually prepared by an extraction process using water, an organic solvent or a mixed solvent thereof, An extract of a mixed solvent, a dry powder thereof, or any of the forms formulated using the same. In addition, the above-mentioned extract also includes fractions obtained by fractionating the extract obtained through the above extraction process.

The composition of the present invention is used in combination with black garlic extract and Chimma extract.

In this study, Gastrodia elata is parasitized in the roots of plants in forests with many humic substances. There is no leaf and potato stalks. The trunk is long elliptical, with an undecimated frame, and the stem is characterized by a small brown leaf with a reddish brown color. As used herein, roots are mainly used, but seed roots, leaves, stems, roots and seeds may also be used as long as they include physiological activity.

The composition according to the present invention significantly increased the content of ergothioneine as a useful ingredient as compared with that contained in the starch alone because of the combination of black garlic and Chumma extract.

Ergotothionein is a kind of natural amino acid that is biosynthesized only in fungi. Since animals, plants, and humans can not synthesize ergotioneine themselves, it is reported that they accumulate mainly in humans through animals consuming mushrooms and ergothionein . In addition, it has recently been reported that aging progresses rapidly in cells lacking ergotioneine. Among the Ganoderma lucidum extract, ergot thionine content is about 2.0 ~ 2.5μg / dry · g (based on dry weight) and shows the difference in content of ergothionein depending on the cultivation area. Ergothioneine is partly contained in certain cells such as human blood, skin and reproductive cells. Ercotionein present in blood is effective in the treatment of diseases caused by impaired cerebral blood flow (AJ Weigand, Thesis, The Pennsylvania State University, 2009; TH Nguyen et al., Food Chemistry, 133, 585-591, 2012).

In the composition according to the present invention, the black garlic and the chimney may be included at a weight ratio of about 1 to 0.01 to 100, in one embodiment about 10: 1.

According to the present invention, black garlic and horse chestnut can be contained at various weight ratios as long as the content of erucothione, which is an antioxidant component, is increased as a useful ingredient.

The black garlic and chimpanzee extract contained in the composition of the present invention may be separately extracted and mixed, or may be prepared as an extract after being mixed before extraction. In one embodiment, black garlic and chymus are mixed and the extract is prepared therefrom.

The extracts of the present invention can be prepared using conventional methods known in the art of common plant extracts (see, for example, Nomura T, Chem. Org. Naturst., 1988, 64, 87201, etc.). For example, an ultrasonic extraction method, a reflux cooling extraction method, and a hot water extraction method may be used. Further, the extracted extract is then purified using a solvent selected from the group consisting of hexane, dichloromethane, acetone, ethyl acetate, chloroform, The fractionation process can be further performed. Two or more kinds of solvents may be used in the fractionation step, or they may be used individually or in combination. Depending on the polarity of the solvent, an extract of each solvent may be sequentially used.

The extract preparation temperature may be 4 to 120 ° C, but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 30 days, and conventional extraction equipment, ultrasonic pulverization extractor or fractionation unit may be used. The produced extract can then be subjected to vacuum filtration and / or lyophilization to remove the solvent. For example, a solvent is added to black garlic or chymarse at a weight ratio of 1: 1 to 1:20 in a heating circulation type extraction tank, purified water or edible alcohol is added, and the mixture is extracted at about 80 to 100 DEG C for about 6 to about 24 hours and filtered to obtain a filtrate , The same procedure can be repeated several times by adding purified water or edible alcohol again to the residue. The filtrate may be used as it is, or it may be concentrated under reduced pressure with a vacuum concentrator, and then the dried product obtained by hot air or lyophilization may be pulverized by a pulverizer.

For example, the method of extracting black garlic or chimpanzee extract according to the present invention is performed by adding 0.1 to 10 L of water per 100 g of the material. The extraction temperature may be about 80-150 캜, but is not limited thereto. The extraction time is not limited, but may be 30 minutes to several days, and an ordinary extraction device or an ultrasonic pulverizing extractor may be used. The produced extract can then be used by vacuum filtration and freeze-drying.

 In one embodiment, the black garlic and chymus extracts are extracted by hot water extraction, but not limited thereto.

The contents of antioxidant components including ergothioneine are maximized in the combination of black garlic and horse chestnut used in the present invention.

Ergothionein has been reported to be effective in the treatment of diseases caused by impaired cerebral blood flow (AJ Weigand, Thesis, The Pennsylvania State University, 2009; TH Nguyen et al., Food Chemistry, 133, 585-591, 2012), and thus compositions according to the present application may be usefully used for the treatment, prevention, amelioration and / or alleviation of cerebral vascular diseases such as stroke, Alzheimer's, etc.

The term "antioxidant" as used herein refers to a function of inhibiting or inhibiting the oxidation of a biological substance when used together, and is a free radical group or other reactive oxygen (O ₂ ^- , H ₂ O ₂ , OH, HOCL, ferryl, peroxyl, peroxynitrite, alkoxyl ) Or to inhibit the formation of these substances, or to convert these substances into substances with low reactivity, and substances having antioxidative properties are effective for the treatment or prevention of diseases related to oxidation. Methods for measuring antioxidant activity are known, and for example, Blois (Blois, MS 1958. Antioxidant determinations by the use of a stable free radical, Nature, 181: 1199-1200), which measures electron donating ability, It is not limited.

Diseases associated with oxidation, in which substances with antioxidant ability can function effectively, include, but are not limited to, the production of free radical groups, especially oxygen radicals, or reactive oxygen species (ROS) Refers to diseases which include diseases in which symptoms are improved by the administration of conventional free radical inhibitors (eg desferroxamine, scavenger (eg tocopherol, glutathione) or a catalyst (eg superoxide dismutase, catalase).

Diseases related to oxidation include, for example, diseases associated with free radicals, ischemic reperfusion injury, inflammatory diseases, systemic lupus erythematosus, myocardial ischemia or infarction, cerebrovascular disorders (vascular embolism, ischemic stroke, hemorrhagic stroke), spinal trauma, , Autoimmune diseases (such as rheumatoid arthritis, diabetes), cataracts, uveitis diseases, emphysema, gastric ulcers, oxygen toxicity, tumors, hypersecretion, radiation disease, and the like.

Therefore, the composition of the present invention having antioxidant ability is capable of inhibiting the formation of active oxygen, or removing the formed active oxygen, thereby antioxidant action.

The composition of the present invention has a synergistic effect on the antioxidative effect when it is used in combination with black garlic extract and Ganoderma extract. The composition of the present application is derived from natural foods and is not toxic or resistant.

In this respect, the antioxidant composition of the present invention can be provided as a pharmaceutical composition useful for the prevention or treatment of a disease requiring antioxidative function, for example, a stroke caused by a disorder of cerebral blood flow as described above.

The term "treatment ", as used herein, refers to any action that improves or alters the symptoms of a disease caused by an impairment of cerebral vascular flow upon administration of the extract or composition comprising it. Those skilled in the art will be able to ascertain, by reference to the data provided by the Korean Medical Association, the precise criteria of the disease for which the composition of the present invention is effective, .

The term "prophylactic, " as used herein, refers to any action that inhibits or delays the onset of a disease caused by the impairment of cerebral vascular flow by administration of the extract or composition comprising it. It will be apparent to those skilled in the art that the extract of the present invention, which has therapeutic effects on disorders caused by cerebral vascular flow disorders, can prevent the early symptoms of cerebral vascular flow disorders, or such diseases when taken before they appear.

The pharmaceutical compositions comprising the black garlic and chymus extracts of the present invention may be administered simultaneously or sequentially and may be administered alone or in combination with other pharmaceutically active ingredients for the treatment of symptoms of a disease, May be administered concurrently.

The composition comprising the black garlic and chymus extract of the present invention may further contain excipients such as suitable carriers, diluents, preservatives, stabilizers, wetting agents, emulsifiers, solubilizers, sweeteners, colorants, osmotic pressure regulators and antioxidants, . Specific examples include lactose, dextrose, sucrose, sorbitol, mannitol xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone Water, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium, stearate, mineral oil and the like.

The method for administering the pharmaceutical composition for preventing or treating diseases caused by cerebrovascular disorder including the black gum and chymus extract of the present invention can be easily selected according to the formulation and administered to mammals such as livestock, . For example, it may be formulated in the form of powders, tablets, pills, granules, dragees, hard or soft capsules, liquids, emulsions, suspensions, syrups, elixirs, external preparations, suppositories, sterilized injection solutions, Orally or parenterally. In particular, oral administration is preferred.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin And the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid formulations for oral administration include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used diluents, various excipients such as wetting agents, sweeteners, have.

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As a base for suppositories, witepsol, macrogol, tween 61, cacao paper, laurin, glycerol, gelatin and the like can be used.

Further, the pharmaceutical compositions comprising the present black gums and Chimpanzee extract may be prepared using the appropriate methods known in the art or by methods described in Remington ' s Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA And the like.

The dosage of the pharmaceutical composition containing the black gum and the Gamma extract of the present invention may vary depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate and severity of disease, And Chimpanzee extract are usually about 1 to 100 g / day, especially about 10 to 50 g / day, more preferably about 30 g / day, for an adult (60 kg). It will be apparent to those skilled in the art that doses may be additive or subtracted, as the dosage can vary depending on various conditions, and thus the dose is not intended to limit the scope of the invention in any way.

The number of administrations can be administered once or several times a day within a desired range, and the administration period is not particularly limited. In addition, the composition containing the extract of the present invention may be added to an arbitrary food and be routinely ingested as such, in addition to oral administration.

The composition of the present invention comprising black garlic and Ganoderma lucidum extract is a natural extract, so that it can be safely used for a long period of time without toxicity and side effects. Accordingly, the black garlic and chymus extracts of the present invention can be used as a main ingredient, a food additive, and a food additive.

Accordingly, in view of the above, the present invention can be applied to foods, such as health supplements, functional foods, food additives, and the like, which comprise the above-described black garlic extract and Chimma extract of the present invention.

As used herein, the term "food" means a natural or processed product containing one or more nutrients, preferably a state of being ready to be eaten through a certain degree of processing, It is intended to include food, food additives, functional foods and beverages as a meaning.

As used herein, the term "functional food" refers to a food group that has been imparted with added value to function or express the function of the food by physical, biochemical, or biotechnological techniques, , And the body control function of disease prevention and recovery is designed to be fully expressed in the living body, and in particular, includes "health functional food".

As used herein, the term "health supplement food" is a food prepared by using raw materials or ingredients having useful functions in the human body, and is useful for health and other purposes such as controlling nutrients and physiological actions on the structure and function of the human body For the purpose of health assisting to obtain the effect, it refers to a food which is made by processing a specific ingredient as a raw material or by extracting, concentrating, refining, mixing, etc. ingredients contained in a food raw material.

In particular, the functional food or the health supplement food according to the present invention means a food having an antioxidative effect that can improve the symptom of the disease caused by active oxygen compared with before the food is consumed, , And those skilled in the art will be able to know the precise criteria of such diseases and determine the degree of improvement by referring to the data presented by the Korean Medical Association.

The functional food and the health supplement food may further include a food-acceptable food supplementary additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of a functional food.

Foods to which the black garlic and chymus extract of the present invention can be added include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, in the present invention, the food may contain special nutritional foods (e.g., crude oil, spirits, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, fruits, vegetables, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, beverage or food additive may be prepared by a conventional production method.

Drinks in the present application mean a general term for drinking or enjoying a taste, and are intended to include functional beverages. The beverage is not particularly limited as long as it contains, as an essential ingredient, the above-mentioned black garlic and chymus extract of the present invention as an active ingredient in the indicated ratios, and may contain various flavors or natural carbohydrates as an additional ingredient can do. Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and Xylitol, sorbitol, and erythritol. Natural flavors (tautatin, stevia extract, for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above . The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention. In addition, the composition of the present invention may further contain flesh for the production of natural fruit juice, fruit juice drink, and vegetable drink.

In the present invention, the functional beverage is used to control the bio-defense rhythm of the beverage group or beverage composition to which the added value is imparted so that the function of the beverage functions for a specific purpose using physical, biochemical, biotechnological techniques, And the like, means a drink which is processed by being designed so as to sufficiently express the body control function with respect to the living body, and preferably means a symptom of metabolic syndrome, for example, a drink for improving hyperlipemia.

The functional beverage is not particularly limited to the ingredients other than the black bean and chymus extract of the present invention as essential ingredients in the indicated ratios and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and xylitol , Sorbitol, and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, , Organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. These components can be used independently or in combination. The proportion of such an additive is not so important, but may be selected in the range of 0.001 to 20 parts by weight per 100 parts by weight of the extract of the present invention.

In the food for the purpose of antioxidation, the combination of the above-mentioned black garlic and chimpanzee extract of the present invention comprises about 0.01 to 90% by weight, about 0.1 to 80% by weight, about 1 to 70%, about 1 to 50% , About 1% to about 40%, about 1% to about 30%, about 1% to about 20%, but does not exclude the range. For example, the beverage composition may comprise 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml.

The present invention also relates to a method for preparing black garlic and chymus extracts for use in the compositions herein. The method comprises: mixing black garlic and chymosin at a weight ratio of 1: 0.01 to 1: 1 and subjecting the mixture to hot extraction at 70 to 100 DEG C for 1 to 3 hours; And filtering and concentrating the extract.

In one embodiment, the black garlic and chimney are used in a weight ratio of 10: 1, and the hot water extraction is performed at about 85 ° C for about 2 hours. And in one embodiment further stirring in the hot water extraction step.

Hereinafter, embodiments are provided to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited to the following examples.

Example 1 Preparation of Black Garlic and Chumama Extract

Black garlic (BG) was made from domestically grown black garlic. 100 g of dried black garlic and 10 g of dried ground chunks (domestic) were added to 2 L of distilled water and stirred at 85 ° C for 2 hours. The extract was filtered by suction to prepare an extract. The same extraction procedure was repeated for the remaining residues after filtration. The extracts were collected and lyophilized using a lyophilizer (PVTFD10A; ilShin Lab, Seoul, Korea) to obtain a viscous extract, which was then concentrated under reduced pressure with a rotary vacuum evaporator (NN type; LAB Camp, Dajeon, Korea) A lyophilized lyophilizate was obtained at about 6.5%.

Example 2 Analysis of components and efficacy of extracts 1

In order to prepare a sample form suitable for HPLC (High Performance Liquid Chromatography) analysis of the black gum and chimpanzee extract according to Example 1, a volume of 70% methanol corresponding to 20 times the volume of black gum and chymus extract was used as an extraction solvent, Was repeated three times. The extracted solution was concentrated to a volume of 10% and purified by passing through a Florisil cartridge. The purified extract was completely dried and the sample was prepared at a concentration of 1 mg / ml (Dried Extract Weight / 1 ml of 70% aq. MeOH).

As a comparative group, it was found that the total amount of octacosanol (containing 12% of octacosanol) and 2.7% of the main ingredients: mixed ginseng, black ginseng juice, ginseng garlic and roasted garlic extract, Gunma powder and Power G7 (MS Biotech Co., Vitamin B6 hydrochloride (Vitamin B6 82%), Vitamin B6 hydrochloride (Vitamin B6 containing 0.0%), vitamin E powder (containing 50% of vitamin E), zinc oxide 0.075%, baked garlic powder 30%, and black garlic powder 5.03%). The yield of the extract according to the extraction conditions is shown in Table 1. The liquid product was diluted 10 times and used for HPLC analysis. The solid product was extracted with 70% methanol and used for the analysis. The yield of extract of garlic was about 27%.

Tables 2 to 4 show the analytical conditions under HPLC analysis. The reference compounds are Ergothioneine, Eleutheroside B, Eleutheroside E, Myricetin, , Kaempferol, Quercetin, and Luteolin, and the rotation line for the reference compound showed linearity.

Input (mg) Extract (mg) Yield (%) State Fermented horse chestnut (liquid) - - - 10 times dilution Black garlic juice - - - 10 times dilution Garlic 10.0 2.78 27.8 - Baked garlic 4.0 2.61 65.3 - Chunma powder 10.0 1.34 13.4 - Power G7 10.0 4.53 45.3 - Black garlic + chestnut extract - - - 10 times dilution

Parameter Eroteroside B, Eroteroside E Conditions equipment Perkin Elmer Flexar HPLC-PDA column Zorbax RX-C18, 4.6 x 150 mm, 5 μm column Detection wavelength 240 nm

Mobile phase A: CH ₃ CN B: water
7% of A isocratic for 2min ---> 40% of A gradient 13min ---> 60% of A gradient 5min ---> 100% of B isocratic 15min Flow rate 1 ml / min Injection volume 10 μl

Parameter Ergotoneine conditions equipment Perkin Elmer Flexar HPLC-PDA column  Zorbax RX-C18, 4.6 x 150 mm, 5 μm column Detection wavelength 254 nm
Mobile phase A: CH ₃ CN B: water
5% of A isocratic for 20 min Flow rate 1 ml / min Injection volume 10 μl

Parameter Myrcetin, camper roll, quercetin, luteolin detection conditions equipment Perkin Elmer Flexar HPLC-PDA column Zorbax RX-C18, 4.6 x 150 mm, 5 μm column Detection wavelength 370 nm
Mobile phase A: 0.5% Phosphoric acid B: MeOH
50% of A isocratic for 20min ---> 50% of A isocratic 10min Flow rate 1 ml / min Injection volume 10 μl

No Flavonoid Retention Time (min) Regression Equation
One
Ergothionein
1.32 y = 42.3433x10 ³ X -18.402x10 ⁴ R ² = 0.9898 2 Eroteroside B 9.50 y = 1.0393 X R ² = 0.9999 3 Eroteroside E 11.40 y = 1.0757 X R ² = 0.9999 4 Myrrh 2.97 y = 1.1233 X R ² = 0.9898 5 Camper roll 5.46 y = 1.4563 X R ² = 0.9978 6 Luteolin 6.89 y = 1.6323 X R ² = 0.9925 7 Quercetin 9.84 y = 1.0493 X R ² = 0.9991

The measurement results are shown in Table 6. As shown above, myristine, camphorol, luteolin, quercetin, and eroteroside E were not detected in all the samples. In the case of ergothioneine, the fermentation broth of Chamaian milk was 6.86 μg / ml, Showed a content of 92.27 μg / ml. In other words, the content of erythrocyte phosphorus was 92.27 μg / ml, which is very high in combination with black garlic. This shows that the content of erucothione, which is a useful ingredient when the extract of Chunma and black garlic are used together, shows that the composition according to the present invention can be usefully used for the improvement or prevention of diseases related to oxidation.

Flavonoid
Retention Time (min)
Fermentation
Chunma
(μg
/ ml)
Black garlic juice
(μg
/ ml)
onomatopoeia
garlic
(μg
/ ml)
Baked garlic
(μg
/ ml)
Chunma powder
(μg
/ ml)
Power G7
(μg
/ ml) Black garlic + chestnut extract
(μg
/ ml) Ergothionein 1.32 6.86 ND ND ND ND ND 92.27 Eroteroside B 9.50 ND ND ND ND ND 1.55 ND Eroteroside E 11.40 ND ND ND ND ND ND ND Myrrh 2.97 ND ND ND ND ND ND ND Camper roll 5.46 ND ND ND ND ND ND ND Luteolin 6.89 ND ND ND ND ND ND ND Quercetin 9.84 ND ND ND ND ND ND ND

ND: Not Detected

Preparation Example 1. Preparation of tablets

Black Garlic and Chunma extract 200 mg

Lactose 100 mg

Starch 100 mg

Magnesium stearate Suitable amount

The above components were mixed and tableted according to a conventional tablet preparation method to prepare tablets.

Formulation Example 2. Preparation of capsules

Black Garlic and Chunma extract 200 mg

Lactose 50 mg

Starch 50 mg

Talc 2 mg

Magnesium stearate Suitable amount

The above components were mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation Example 3. Preparation of a liquid preparation

Black Garlic and Chunma extract      1000 mg

Sugar 20 mg

Isomer 20 mg

Lemon incense Suitable amount

Purified water is added to the entire 1000 ml

Then, the above components were mixed according to a conventional method for preparing a liquid agent, and then filled in a brown bottle and sterilized to prepare a liquid agent.

Formulation Example 4. Preparation of health drinks

  Black Garlic and Chunma extract     1000 mg

Citric acid 1000 mg

oligosaccharide 100g

Mesyl concentrate 2g

Taurine 1g

Purified water is added to the entire 1000ml

The above components were mixed according to a conventional health drink manufacturing method, and the resulting solution was stirred and heated at 85 DEG C for about 1 hour. The resulting solution was filtered and sterilized in a 2 L sterilized container. The resulting solution was refrigerated, ≪ / RTI >

Formulation Example 5. Preparation of Healthy Foods

  Black Garlic and Chunma extract 1000 mg

Vitamin mixture Suitable amount

Vitamin A acetate 70 mg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 mg

Vitamin C 10 mg

Biotin 10 mg

Nicotinic acid amide 1.7 mg

Folic acid 50 mg

Calcium pantothenate 0.5 mg

Vitamin E 1.0 mg

Mineral mixture Suitable amount

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dicalcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

24.8 mg of magnesium chloride

Although the composition ratio of the above-mentioned vitamin and mineral mixture is relatively mixed with a suitable preparation for health food, the compounding ratio may be arbitrarily modified, and the above ingredients may be mixed according to a conventional method of manufacturing health food, The granular formulation of health food was prepared.

While the present invention has been described in connection with what is presently considered to be the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, .

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. The contents of all publications referred to herein are incorporated herein by reference.

Claims (13)

An antioxidant composition comprising a combination of black garlic and chymus extract.
The method according to claim 1,
Wherein said black garlic and chymus extract are contained in a weight ratio of black garlic extract of 10: 1.
The method according to claim 1,
Wherein said black garlic and chymus extracts are extracted by hot water extraction.
4. The composition according to any one of claims 1 to 3, wherein the composition is provided as a pharmaceutical composition for the treatment or prevention of cerebral blood flow disorder disorder.
4. The antioxidative composition according to any one of claims 1 to 3, wherein the composition has an increased content of erythrothionine due to the combination of the black garlic and the chromatin.
Wherein the food composition is provided as a health supplement, a functional food, a beverage or a food additive.
A method for producing black garlic and chum salmon extract,
Mixing black garlic and horse chestnut;
Extracting the mixture;
Alternatively, instead of the two steps,
Providing a black garlic extract and a chewing gum extract;
Mixing the black garlic extract and the chymus extract; And
Filtering and concentrating the extract.
A method for producing black garlic and ginseng extract.
8. The method according to claim 7, wherein the black garlic and the horse chunks are mixed at a weight ratio of 10: 1.
8. The method of claim 7, wherein the extraction is hot water extraction, and the hot water extraction is performed at 70 to 100 DEG C for 1 to 3 hours.
10. The method of claim 9, further comprising stirring in the hot water extraction step.
A method for producing black garlic and chum salmon extract, wherein the antioxidant component is enhanced by mixing black horse with chamaese.
12. The method according to claim 11, wherein the black garlic and the chimney are mixed at a weight ratio of 10: 1.
12. The method according to claim 11, wherein the extraction is hot water extraction, and the hot water extraction is performed at 70 to 100 DEG C for 1 to 3 hours.