KR101498075B1 - Composition and health functional food containing extracts of sorghum bran - Google Patents

Abstract

More particularly, the present invention relates to a composition having an antioxidative activity, which comprises an extract of Aspergillus oryzae, which has an antioxidative activity higher than that of a mulberry extract or / and a mulberry extract, Pharmaceutical compositions and / or health functional foods.

Description

[0001] The present invention relates to an antioxidant composition and a functional food containing extracts of sorghum bran,

More particularly, the present invention relates to a composition having an antioxidative activity, which comprises an extract of Aspergillus oryzae, which has an antioxidative activity higher than that of a mulberry extract or / and a mulberry extract, Pharmaceutical compositions and / or health functional foods.

Sorghum (Sorghum bicolor L. Moench, sorghum) is a perennial herb that has been consumed mainly for edible purposes. Tropical Africa is the country's most cultivated land in the arid region and cultivated as grain sorghum, sorgo and broom-corn depending on the application, and in Asia, Africa and Central America It is a food resource being cultivated. Syrup is the world's fifth largest producer of rice, barley, wheat and corn in the world with a production of 57,772,000 tons. It contains a large amount of active ingredients such as dietary fiber and phenolic compounds. Phenolic compounds are composed of Condensed tannin, Flavonoid, Phenolic acid, and most of them are known as flavonoids. These phytochemicals are known to exhibit a variety of health functional activities beneficial to the human body. Polyphenol extracts of Acerola have been reported to have strong antimutagenic properties.

In addition, the phenolic compounds in the sorghum have varying degrees of content depending on the variety and have been reported to exhibit various health functional activities. As a result of comparing the electron donating ability, reducing power, antimicrobial activity and α-glucosidase inhibitory activity of 22 domestic varieties, the difference in the physiological activity between varieties was large, Has a higher electron donating ability than synthetic antioxidants such as BHT and BHA. In addition, in the previous studies, 25 kinds of sorghum were extracted with methanol, and the antioxidative and antimicrobial activities were tested after sequential solvent fractionation. As a result, the activity varied according to the species of sorghum.

Antioxidation refers to the prevention of many oxidative effects in the body. Oxidation refers to the phenomenon that when various kinds of unsaturated fatty acids present in the body are oxidized by oxygen, a substance called lipid peroxidation is produced, and when it is increased, aging phenomenon is promoted. Human diseases and aging can be classified into O ₂ ^- (superoxide), NO · (nitric oxide), NO 2 · (nitrogen dioxide), OH · (hydroxyl), ROO · (proxyl) , NO ₃ · (peroxynitrite) radicals and the like. In vivo, superoxide dismutase (SOD), catalase (CAT) as a means for defending the living body from free radicals such as harmful radicals, catalase and antioxidant enzymes such as glutathione peroxidase (GSHpx) and vitamin E and glutathione are present to protect the living body from oxidative damage by eliminating free radicals. However, when such an antioxidant defense system declines to such an extent that free radical formation can not be controlled, the oxidative stress and damage of the tissue are promoted, and the excess free radicals and lipid peroxides produced in the body are oxidized by oxidative stress such as protein oxidation and DNA damage . Therefore, many antioxidants have been developed to reduce such oxidative stress.

Such antioxidants may be used for the prevention of inflammatory diseases such as dermatitis, sore throat, tonsillitis, fibromyalgia, rheumatoid arthritis, shoulder joint inflammation, myositis, hepatitis, cystitis and nephritis, aging, fatigue, leukemia, breast cancer, colon cancer, prostate cancer, Skin cancer, non-metastatic lung cancer, uterine cancer, black copper, colorectal cancer, and central nervous system cancer.

However, the conventional synthetic antioxidants showed high antioxidative activity as an antioxidant. However, since they have a problem of safety to human body as a medicine, recently, they have developed a natural antioxidant which is more safe and has excellent antioxidative effect in various herbal medicines or edible plant extracts We are making efforts to do so. However, conventional antioxidants derived from natural products have no problem of stability to human body, but have low antioxidative activity and thus are not suitable for use as pharmaceuticals.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide an antioxidant composition which is more harmless to human body than conventional synthetic antioxidants and has improved antioxidative activity than natural- It is also an object of the present invention to provide an antioxidant composition and / or a health functional food having a high content of tannins and polyphenols, including a part having high antioxidative activity during transplantation.

The present invention provides an antioxidant composition comprising a chafflet extract.

According to a preferred embodiment of the present invention, the chestnut extract may contain at least one of the group consisting of tannins, flavonoids and polyphenols.

According to another preferred embodiment of the present invention, the antioxidant composition may contain 10 to 70% by weight of the chestnut extract.

According to another preferred embodiment of the present invention, the chopped chicory extract may be chestnut extract of Chrysanthemum morifolium, which is deposited at the National Institute of Agricultural Science and Technology of Korea, KACC98013P.

In addition, the present invention provides an antioxidant health functional food comprising a chaff endowed extract.

According to a preferred embodiment of the present invention, the barnyardgrass extract may have an ABTS radical activity of 50-120 mg Trolox equivalent / g sample.

According to another preferred embodiment of the present invention, the chestnut extract may contain 10 to 30 mg of tannin and 20 to 80 mg of polyphenol.

According to another preferred embodiment of the present invention, the antioxidant health functional food may have antioxidant or fatigue recovery activity.

According to another preferred embodiment of the present invention, the chopped chicory extract may be chestnut extract of Chrysanthemum morifolium, which is deposited at the National Institute of Agricultural Science and Technology of Korea, KACC98013P.

Further, the present invention relates to a process for producing a mixture comprising: a first step of mixing a bran and an extraction solvent to prepare a mixture; And 2) extracting the extract from the mixture; ≪ RTI ID = 0.0 > a < / RTI >

According to a preferred embodiment of the present invention, the extraction solvent may be at least one kind selected from the group consisting of water, acetone and lower alcohol.

According to another preferred embodiment of the present invention, the method for extracting the extract may be performed by treating the mixture with ultrasound at 40 to 80 Hz for 1 to 5 hours.

Hereinafter, terms of the present invention will be described.

The term " harvested bran "of the present invention means a byproduct produced in the course of harvesting the harvested rice after harvesting the harvested rice, and may include all the byproducts generated during the harvesting.

The "lower alcohol" of the present invention means any one or more of the group consisting of C ₁ -C ₄ alcohols.

It is an object of the present invention to provide an antioxidant composition which is more harmless to human body than conventional synthetic antioxidants including antimicrobial extracts of sorghum and has antioxidative activity which is higher than antioxidants derived from natural materials in the past.

It is also an object of the present invention to provide an antioxidant composition and / or a health functional food having a high content of tannins and polyphenols, including a part having high antioxidative activity during transplantation.

In addition, it is possible to provide a cultivar having an excellent content of antioxidative active substance among various cultivars having various contents of antioxidative active substances.

Furthermore, it is possible to provide a pharmaceutical composition for antioxidant and / or a health functional food having an advantage and antioxidative activity in that it has been used in the past because it has been used for a long time.

FIG. 1 shows the results of ABTS radical scavenging activity measured in Experimental Example 1. FIG.
Fig. 2 shows the results of polyphenol, tannin and antioxidant activity measured in Experimental Example 2. Fig.
Fig. 3 shows the content of the antioxidative active substance of the mulberry bran extract as measured in Experimental Example 3-1.
Fig. 4 shows the ABTS antioxidant activity and DPPH antioxidant activity of the chestnut extract obtained in Experimental Example 3-2.
FIG. 5 shows the results of ABTS antioxidant activity and polyphenol content of the mulberry bran extract of Experimental Example 4.

Hereinafter, the present invention will be described in more detail.

As described above, sorghum is the highest antioxidant activity of polyphenols and antioxidant components in cereals, and thus various studies on the health function of sorghum have been carried out as described above. However, in order to effectively utilize the antioxidative activity of sorghum in an industrial aspect, it is inevitable to develop a water extract or a processed product having superior antioxidant activity.

Accordingly, the present invention provides a pharmaceutical composition for antioxidants and / or a health functional food containing the chestnut extract and provides a method of effectively utilizing the antioxidative activity of citron in an industrial aspect, A pharmaceutical composition for antioxidants having an antioxidative activity and / or a health functional food can be provided.

The antioxidant composition and / or the health functional food of the present invention include a cherry vinegar extract.

The above-mentioned harvested horse means a by-product generated in the process of threshing the harvested ear and then grinding the grain, and may include all the by-products generated in the process of harvesting the grain.

As can be seen in Figure 1, the ABTS (2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical activity was 13.9-25.4 Trolox equivalent / g sample (3.9 ~ 4.7 mg Trolox equivalent) / g sample (g sample) Obtained 3 ~ 25 times higher extract than ginseng extract can do.

The transfer is not particularly limited as long as it can be normally cultivated and purchased, but it is preferable to use the transfer number KACC98013P deposited on October 15, 2012, to the depository agency Agricultural Genetic Resources Center.

FIG. 2 shows the antioxidant activity and antioxidative activity of various varieties of mulberry bran extract. In the case of using the silkworm No. KACC98013P, 1.2 ~ 8 times more tannin And / or < / RTI > polyphenols.

In addition, the above-mentioned water-borne chestnut extract is not particularly limited as long as it contains an antioxidative active substance that can be present in a plant, but it may preferably contain tannin, flavonoid and / or polyphenol. have.

In addition, the watercress extract may contain 10 to 30 mg of tannin and 20 to 80 mg of polyphenol.

In addition, the antioxidant activity of the above-mentioned barnyardgrass extract may have an ABTS radical activity of 50-120 mg Trolox equivalent / g sample (g sample). This results in a gross excretion of trolox equivalent / g sample (g / sample) or 3.9 ~ 4.7 mg trolox equivalent / g sample (g sample) of 13.9-25.4 mg, It is 3 ~ 25 times higher than that of Acanthopanax senticosus extract.

As shown in FIG. 2, the ABTS radical activity of the chestnut bark extract, which was obtained from Susungwoo, Milyang 1 or Milyang 4, which has the deposit number KACC98013P, 22 times higher.

Although the content of the antioxidant composition is not particularly limited as long as it contains the chestnut kernels, the antioxidant composition preferably contains 10 to 70% by weight of the chestnut extract in the weight of the antioxidant composition.

The antioxidant composition according to the present invention can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols or the like, oral preparations, suppositories or sterilized injection solutions according to conventional methods Can be used.

More specifically, when formulating the composition, it can be prepared using a diluent or an excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, (sucrose), lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

Examples of liquid formulations for oral use include suspensions, solutions, emulsions and syrups. In addition to water and liquid paraffin which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like have.

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The amount of the dungberry extract according to the present invention may vary depending on the age, sex and body weight of the patient, but is generally in the range of 0.01 to 500 mg / kg, preferably 0.1 to 100 mg / kg per day, Can be administered separately. In addition, the dose of the watercress extract may be increased or decreased depending on the route of administration, degree of disease, sex, weight, age, and the like. Accordingly, the dosage is not limited in any way to the scope of the present invention.

There is no particular limitation on the kind of the antioxidant health functional food including the chestnut extract of the present invention. Examples of the food to which the chestnut extract can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, Tea, a drink, an alcoholic beverage, and a vitamin complex, and can be used in the form of pills, powders, granules, infusions, tablets, capsules or beverages, and includes health functional foods in a conventional sense.

At this time, in the case of the antioxidant health functional food of the present invention, the amount of the dunberry extract in the food or beverage can be generally 0.01 to 15% by weight of the weight of the antioxidant health functional food and 100 ml of the health beverage composition May be added in a proportion of 0.02 to 10 g, preferably 0.3 to 1 g.

The health beverage composition of the present invention is not particularly limited to a liquid component other than the above-mentioned water-borne chestnut extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages . Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc., disaccharides such as maltose, sucrose, etc., polysaccharides such as dextrin and cyclodextrin Conventional sugars and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. As natural flavors other than those described above, natural flavors (such as tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin)) and synthetic flavors (saccharin, aspartame, etc.) have.

In addition to the above, the health functional food of the present invention may contain various kinds of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the health functional foods of the present invention may contain flesh for the production of natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination.

Further, the present invention relates to a process for producing a mixture comprising: a first step of mixing a bran and an extraction solvent to prepare a mixture; And a second step of extracting the extract from the mixture.

First, the mixture is prepared by mixing the bran and extraction solvent in one step.

The mixing ratio of the above-mentioned water-extracting and extracting solvent is not particularly limited as long as it is conventionally used for the production of plant extract. Preferably, 50 to 1,000 parts by weight of the extraction solvent may be mixed with 100 parts by weight of water- And 70 to 500 parts by weight based on 100 parts by weight of the bran.

The extraction solvent is not particularly limited as long as it is conventionally used in the production of plant extracts. Preferably, one or more of acetone, lower alcohol and water may be used. More preferably, acetone or ethanol may be used. have.

As shown in Table 1 of Experimental Example 3-3, the use of ethanol or acetone as the extraction solvent is preferable to the use of hexane as the extraction solvent, and the most preferred ethanol extract is the antioxidant It was found that a large amount of active and antioxidant active substances were contained.

Next, step 2 extracts the extract from the mixture.

The method for extracting the above extract is not particularly limited as long as it is a method used for preparing a plant extract. Preferably, the extract is subjected to shaking extraction, ultrasonic extraction, reflux extraction, and political extraction And more preferably, it can be extracted by shaking extraction or ultrasonic extraction, and most preferably, ultrasonic extraction can be performed.

In addition, in the case of extracting using the ultrasonic extraction method among the above extraction methods, it is preferable to extract ultrasonic waves of 40 to 80 Hz and 1 to 5 hours.

If ultrasonic wave of less than 40 ㎐ is used, it may cause difficulties in extracting antioxidant activity and antioxidant activity contained in the bran. When ultrasonic wave exceeding 80 ㎐ is used, the antioxidant activity The active and antioxidant active substances may be destroyed.

In addition, in the case of extracting using the shaking extraction method among the above extraction methods, shaking culture can preferably be carried out at 15 to 60 ° C for 24 to 96 hours.

If shaking culture is carried out at a temperature lower than 15 캜, there may arise a problem that the antioxidative activity and the extraction yield of the antioxidative active substance contained in the bran are lowered, and the shaking culture is carried out at a temperature exceeding 60 캜 The turbidity due to the starch gelification may increase during the extraction, or the heat-sensitive component of the antioxidant may be destroyed.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

Example  One. For a while  Manufacture of ethanol extract

The seedlings were thawed using a test stripper during a new variety, KACC98013P, deposited on Oct. 15, 2012, to the Depositor Agricutural Genetic Resource Center to obtain seeds. Then, 10 g of 100% ethanol was added to 2 g of perilla seedlings obtained in the course of preparing the seeds for the period of the test period, and the seeds of DAIHAN SCIENTIFIC CO. Ultrasonic waves of 50 to 60 Hz were used for ultrasonic wave extraction using a WiseClean of Wako Pure Chemical Industries, Ltd., and 10 ml of ethanol was further added thereto. After filtration, the filtrate was diluted to 20 ml to obtain an ethanol extract of rice bran.

Example  2. For a while  Manufacture of Acetone Extract

A methylated acetone extract was obtained during the same manner as in Example 1, except that 100% acetone was used as the extraction solvent.

Example  3. Shindang extract manufacturing

The extract was further subjected to shaking extraction at 25 DEG C for 48 hours after addition of ethanol to distilled water.

The shaking extraction was carried out using DAIHAN SCIENTIFIC CO. Shaking at a speed of 240 to 250 RPM using a Wise Shaker (SHO-2D).

Comparative Example  One. For a while  Grain extract

A megarad extract was obtained during the same manner as in Example 1, except that the extract was prepared using the horseradish juice during the period obtained in Example 1.

Comparative Example  2. For a while  Myrtle extract

A mushroom extract was obtained in the same manner as in Example 1, except that the extract was prepared using the mace of witchcraft during the period before the operation of Example 1.

Comparative Example  3. A gold rally  Chestnut extract

(Purchased from Kwangbok Agricultural Products Co., Ltd.) was used in place of liquorice, and a golden bran bran extract was obtained in the same manner as in Example 1.

Comparative Example  4. A gold rally  Myrtle extract

A golden wilt extract was obtained in the same manner as in Comparative Example 3, except that the extract was prepared using the wreaths of gold wool before compaction of Comparative Example 3.

Comparative Example  5. A gold rally  Grain extract

A golden wilt extract was obtained in the same manner as in Comparative Example 3, except that the extract was prepared using the golden wax obtained in Comparative Example 3.

Comparative Example  6. For a while  Bite Hexane  extract

The hexane extract was obtained during the same manner as in Example 1, except that hexane was used as the extraction solvent.

Comparative Example  7. Miryang No.2  Chestnut extract

Except for using Milyang No. 2 instead of Mishyu, the extract of Milyang 2 was obtained in the same manner as in Example 1.

Comparative Example  8. White chimney  Chestnut extract

White rye bran extract was obtained in the same manner as in Example 1 except that white rye (purchased from Goesan) was used instead of ryegrass.

Comparative Example  9. Milyang 1  Chestnut extract

Except for using Milyang No. 1 instead of Mishyu, the same procedure as in Example 1 was carried out.

Comparative Example  10. Milyang 4th  Chestnut extract

Except that Milyang 4 was used instead of Maceae. The extracts of Milyang 4 and 5 were obtained in the same manner as in Example 1.

Experimental Example  1. Antioxidant activity measurement

The ABTS radical scavenging activity of the extracts obtained in Example 1 and Comparative Examples 1 to 5 was measured in order to examine the antioxidative activity.

The ABTS radical scavenging activity was measured by the ABTS cation decolorization assay. ABTS (2,2'-Azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid) 7.4 mM and 2.6 mM potassium persulphate were allowed to stand in the dark for one day to form ABTS cations And the ABTS solution was diluted with ethanol using molar extinction coefficient (ε = 3.6 × 10 ⁴ M ^-1 cm ^-1 ) such that the absorbance value was 1.4 to 1.5 at 735 nm. To 1 ml of the diluted ABTS solution, 50 쨉 l of the extract was added and after 30 minutes, the change in absorbance was measured. The measured ABTS antioxidant activity is shown in FIG.

(Comparative Example 1 and Comparative Example 5), fern extract (Comparative Example 2 and Comparative Example 4), bran extract (Example 1 and Comparative Example 3) , Respectively. The antioxidant activity was shown to be 3 ~ 6 times higher in weevil extract and 3 ~ 4 times higher than weevil extract in comparison with weevil extract. Especially, the extracts of P. japonicus showed 7.4 times higher antioxidant activity than the extracts of P. japonicum.

As can be seen from the above results, the mulberry extract had higher antioxidative activity than the mulberry extract and the mulberry extract. Therefore, in order to utilize the antioxidative activity of the mulberry tree, it is more preferable to use millet Could know.

Experimental Example  2. Determination of antioxidant and antioxidant contents

The contents of tannin and polyphenol, which are antioxidants, of the bamboo extracts obtained in Example 1, Comparative Example 3 and Comparative Examples 7 to 10 were measured.

The total polyphenol content was analyzed on the principle that the Folin-Ciocalteu phenol reagent was reduced to molybdenum blue as a result of being reduced by the polyphenolic compound of the above-mentioned extract. 50 μl of a 50% Folin-Ciocalteu reagent (Sigma-Aldrich, St. Louis, Mo., USA) was added to 50 μl of the above-described watercress extract and 1 ml of a 2% Na ₂ CO ₃ solution was added thereto. . After 30 minutes, the absorbance of the reaction solution was measured at 750 nm. A calibration curve was prepared using gallic acid (manufactured by Sigma-Aldrich) as a standard, and a gallic acid equivalent (GAE) / g (dry sample) on a dry basis.

The total tannin content was calculated using tannic acid as a standard. That is, 1 ml of 95% ethanol and 1 ml of distilled water were added to 1 ml of the above-mentioned chestnut extract, and the mixture was shaken well. Then, 1 ml of 5% Na ₂ CO ₃ solution and 0.5 ml of 1 N Folin-ciocalteu reagent were added and developed for 60 minutes at room temperature. Then, the absorbance was measured at 725 nm.

The antioxidant activity was measured by the ABTS cation decolorization assay and the same procedure as in Experimental Example 1 was carried out.

The measured polyphenol, tannin and antioxidant activity results are shown in FIG.

As can be seen in FIG. 2, the highest concentration of ABTS antioxidant activity was found in all of the varieties (Example 1), 1.9 times of the extract of Golden Rice bran extract (Comparative Example 3), white raspberry bark extract 8).

In addition, the content of tannin and polyphenol also showed the same tendency as that of ABTS antioxidant activity, while the extract of mackerel bark (Example 1) was about 1.9 times higher than that of gold bell pepper extract (Comparative Example 3) Which is 8 times as many as that of the conventional method.

From the above results, it can be seen that, during the period, it contains an antioxidant activity and a large amount of antioxidant active substance which are superior to other varieties. Therefore, it was confirmed that the most suitable variety to use the antioxidant activity of sorghum was the most preferable.

Experimental Example  3. Measurement of organic solvent extraction efficiency

The extraction efficiency of the methanol extract, the methanol extract and the methanol extract during the period from the results of Examples 1 and 2 and Comparative Example 6 (hereinafter collectively referred to as the "water extracts") were measured Respectively.

Experimental Example  3-1: Measurement of content of antioxidant active substance

The content of polyphenol contained in the above-mentioned watercress extracts was measured in the same manner as in Experimental Example 2, and the measurement results are shown in FIG.

The content of flavonoid was determined by adding 1 ml of distilled water and 75 μl of 5% NaNO 2 to 250 μl of the extract, adding 150 μl of 10% AlCl ₃ 6H ₂ O 5 minutes, allowing to stand for 6 minutes, and adding 500 μl of 1 N NaOH. After 11 minutes, the absorbance of the reaction solution was measured at 510 nm. The calibration curve was prepared using a standard substance (+) - catechin (Sigma-Aldrich) and measured by a method represented by mg catechin equivalent (CE) / g (dry basis). The measurement results are shown in FIG.

As can be seen in FIG. 3, during the extraction of Example 1 using ethanol, the ethanol extract of Mugwort was 1.1 to 1.3 times higher than that of acetone (Example 2) and 5 to 10 times higher than that of hexane (Comparative Example 6) Of polyphenols and flavonoids. In addition, it was confirmed that the Acanthopanax senticosus extract (Example 2) extracted with acetone contained 5 to 8 times more polyphenols and flavonoids than the Acanthopelust extract extracted with hexane (Comparative Example 6).

Experimental Example  3-2: Antioxidant activity measurement

The antioxidative activity and DPPH antioxidant activity of ABTS were measured in order to measure the antioxidative activity of the above - mentioned.

The antioxidative activity of ABTS was performed in the same manner as in Experimental Example 1 above. In addition, DPPH antioxidant activity was measured by adding 0.2 ml of a 0.2 mM DPPH solution (dissolved in 99.9% ethanol) to 0.2 ml of the sample, and then measuring the absorbance at 520 nm exactly after 30 minutes. The scavenging activity of DPPH radicals was measured by the method expressed in mg TE (Trolox equivalent antioxidant capacity) / g (dry basis). The measured antioxidant activity is shown in Fig.

As can be seen from FIG. 4, the ethanol extract of the rice celery during the extraction of Example 1 using ethanol showed an antioxidative activity 1.4-2.0 times higher than that of acetone (Example 2). In addition, antioxidative activity was not measured in the method of measuring ABTS antioxidant activity and DPPH antioxidant activity of the chestnut extract (Comparative Example 6) extracted with hexane.

Experimental Example  3-3: Measurement of extraction efficiency

A method of measuring the weight of the residue by subjecting the extracted extract to vacuum drying and freeze-drying processes in the same manner as in Examples 1 to 2 and Comparative Example 6, except that 10 g of the extracting efficiency of the above-mentioned barnyardgrass extracts was used Respectively. The calculated extraction efficiencies are shown in Table 1 below.

division Example 1
(Extraction solvent: ethanol) Example 2
(Extraction solvent: acetone) Comparative Example 6
(Extraction solvent: hexane) Extraction efficiency (%) 10.1 ± 0.117 5.8 ± 0.353 4.5 ± 0.127

As shown in Table 1, it was confirmed that the extraction efficiency of Example 1 in which the extract was extracted using ethanol as the extraction solvent was higher than that in the case of using acetone or hexane, and Example 2 in which the extract was extracted using acetone Extraction efficiency was 1.3 times higher than that of Comparative Example 6 using hexane as an extraction solvent.

As can be seen from the above experiments, ethanol is the most preferable solvent for extracting antioxidants, and acetone is also preferable for extracting antioxidants.

Experimental Example  4. Determination of antioxidant activity and polyphenol content by extraction method

The ABTS antioxidant activity and polyphenol content of the cherry safflower extract obtained in Example 1 and Example 3 were measured.

The ABTS antioxidant activity was measured in the same manner as in Experimental Example 1, and the polyphenol content was measured in the same manner as in Experimental Example 2. The measured ABTS antioxidant activity and polyphenol content are shown in FIG.

As shown in Fig. 5, the antioxidative activity component (polyphenol) and the antioxidant activity (antioxidant activity) of the extract (Example 1) obtained by ultrasonic extraction compared to the extract obtained by shaking (Example 3) ABTS antioxidant activity) was increased.

Therefore, it was found that ultrasonic extraction is preferable to shaking extraction as a method of extracting an antioxidative active substance.

As can be seen from Examples, Comparative Examples and Experimental Examples, it was confirmed that polyphenols, polyflavonoids and tannins, which are antioxidative active substances, are contained in a large amount in the shrubs than in the weevil or wheat. In addition, ethanol is the most preferable solvent for extracting antioxidative active materials from sorghum, and it is found that acetone is preferable for extracting antioxidative active substances. Furthermore, it is desirable to obtain antioxidative active substances by using these antioxidative active substances in a large amount of antioxidative active substances in Mesosu, Milyang 1 and Milyang 4 during the transplanting of various varieties. In addition, Was most preferable. In addition, it has been found that, in the method of extracting a fermented antioxidative active substance, it is more preferable to perform shaking extraction with ultrasonic extraction.

[ Manufacturing example ]

Manufacturing example  1: Preparation of pharmaceutical composition

An example of a pharmaceutical composition containing a chaff endowed extract is described, but the present invention is not intended to be limited thereto but is specifically described.

The chestnut extract used in the following preparation example was prepared by the same method as in Example 1 except that the extract was used.

Manufacturing example  1-1. Sanje  Produce

2 g < RTI ID = 0.0 >

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

Manufacturing example  1-2. Manufacture of tablets

100 mg of the bran extract of Example 1

Corn starch 100 mg

100 mg of milk

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Manufacturing example  1-3. Preparation of capsules

100 mg of the bran extract of Example 1

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

Manufacturing example  1-4. Manufacture of rings

1 g < RTI ID = 0.0 >

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

Manufacturing example  2: Preparation of health functional foods.

The preparation of a health functional food containing the chestnut extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically explained.

The chestnut extract used in the following preparation example was prepared by the same method as in Example 1 except that the extract was used.

Manufacturing example  2-1. Manufacture of Health Functional Foods

3000 mg of the bran extract of Example 1

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

10 mg vitamin C

Biotin 10 μg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Potassium citrate 90 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is relatively mixed with a suitable preparation for health food, the compounding ratio may be arbitrarily changed, and the above ingredients may be mixed according to a conventional method for producing healthy food , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Manufacturing example  2-2. Manufacture of health drinks

3 g < RTI ID = 0.0 >

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, Of health functional beverage composition.

Although the composition ratio is relatively mixed with the ingredients suitable for the favorite beverage as a preferable preparation example, the blending ratio may be arbitrarily modified according to the local or national preference such as the demand class, demand country, use purpose, and the like.

National Institute of Agricultural Science KACC98013P 20121015

Claims (12)

An antioxidant composition comprising a chafflet extract of KACC 98013P, National Center for Agronomy, National Institute of Agricultural Science and Technology. 2. The antioxidant composition according to claim 1, wherein the chopped strawberry extract comprises at least one member selected from the group consisting of tannins, flavonoids and polyphenols. The antioxidant composition according to claim 1, comprising from 10 to 70% by weight of a water-borne chestnut extract in the total weight of the antioxidant composition. delete Antioxidant and health functional food containing the chestnut extract of KACC98013P, Accession No. KACC98013P, Center for Agricultural Genetic Resources, National Academy of Agricultural Science. The antioxidant health functional food according to claim 5, wherein the antioxidant health functional food has antioxidant or fatigue recovery activity. [6] The antioxidant health functional food according to claim 5, wherein the watercook extract comprises 10 to 30 mg of tannin and 20 to 80 mg of polyphenol. 6. The antioxidant health functional food according to claim 5, wherein the water-borne chestnut extract has an ABTS radical activity of 50-120 mg Trolox equivalent / g. delete The first step of preparing a mixture by mixing the bran and extraction solvent of KACC 98013P, National Institute of Agricultural Science and Technology, And
2) extracting the extract from the mixture;
Of the National Institute of Agricultural Science and Technology, National Institute of Agricultural Science and Technology, KACC98013P. [Claim 10] The method according to claim 10, wherein the extraction solvent is at least one member selected from the group consisting of water, acetone, and lower alcohol. 11. The method according to claim 10, wherein the step (2) is carried out by sonication at 40 to 80 Hz for 1 to 5 hours.

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