KR20160055748A - Barley sprout tea having increased content of antioxidative or hypoglycemic components - Google Patents

Abstract

The present invention relates to a method for making barley sprout tea with increased content of antioxidative or hypoglycemic components comprising the steps of; harvesting the top part of barley sprout cultivated for 3 to 5 days at 18 to 26°C after seeding; and roasting the harvested barley sprout at 180 to 250°C. Moreover, the present invention relates to barley sprout tea manufactured by the method, a method for making antioxidative or hypoglycemic components comprising extracting the barley sprout tea with a water-containing solvent, and antioxidative, antiphlogistic or hypoglycemic food, cosmetic and pharmaceutical compositions including extract of the barley sprout tea as an effective ingredient. The objective of the present invention is to facilitate intake without refusal by providing a tea-type barley sprout.

Description

[0001] The present invention relates to a barley sprout having increased antioxidant or hypoglycemic active ingredient content,

The present invention relates to a method of harvesting roasted barley, And a second step of boiling the harvested germanium barley at 180 to 250 DEG C, a method for producing a germanium barley tea having an increased antioxidant or hypoglycemic active ingredient content, a germanium barley tea prepared by the method, A method for producing an antioxidant or hypoglycemic active ingredient comprising extracting the extract with a water-containing solvent, and a method for producing an antioxidant, anti-inflammatory or hypoglycemic food composition, a cosmetic composition and a pharmaceutical composition .

Tea (tea) is used for edible and medicinal purposes along with the history of mankind. Gradually, its efficacy has been revealed, and it has been established as a drink of everyday life. Now, with coffee and cocoa, have. In addition, modern society is becoming more aware of health due to the development of medical technology and the economic development along with aging. The phenomenon of pursuing such high quality of life is not only Korea but also a global trend, which is expected to accelerate further in the future.

It has been known since ancient times that the content of inorganic substances such as calcium, magnesium and potassium is high, and the content of vitamin B1 and vitamin C is high, and it is known as a food source which is also excellent in nutrition. Also, it has antioxidant, anti- The industrial availability of health functional foods and medicines is increasing because it contains functional secondary metabolites such as saponarin, lutonarin and isovitexin. In Japan and the United States, dried products of dried barley leaves are developed and sold as health foods.

On the other hand, skin aging, various cancers, and inflammation were found to be the main cause of reactive oxygen species (ROS). Therefore, studies are being conducted to develop an antioxidant capable of removing such harmful active oxygen as a material for many health functional foods and the like. It has been reported that soybean barley, a young barley leaf, contains a large amount of polyphenols, and these polyphenols have an excellent antioxidative effect for removing harmful active oxygen which is harmful to human body.

The present invention relates to a method for inhibiting neuraminidase activity and a pharmaceutical composition for the prevention and treatment of influenza virus infection diseases, Methods for producing wheat or barley tea, beverage for diabetic patients, analysis of water-soluble phenol compounds of barley leaves, tofu containing barley leaf powder, and the like. In addition, a study on the effect of cholesterol on the improvement of cholesterol by using barley, the effect of barley on the lipid content and hepatic lipid metabolism-related enzyme activity in high fat fed mice, and the antioxidative effect and hyperlipemia inhibitory effect of barley extract using rabbit atherogenic effect model .

Other studies using young barley leaves have disclosed the methanol extraction and activity assays of saponin and isobutyric acid, the barley young leaf antioxidants, and the LC analysis of polyphenols with antioxidant activity of barley leaves.

So far, most studies on shoot barley (young barley leaves, barley) have mainly concerned with the method of using the crude extracts for the manufacture of products and the assay of physiological activities such as antioxidant activity. The content of polyphenols as an active ingredient was measured from the crude extract, and saponin, rutonin, and glycosides were separated and identified.

As to the activity of the antioxidant composition, the components of the composition isolated from various plants or microorganisms exhibiting antioxidative effects were analyzed. As a result, it was found that a large number of polyphenols and / or flavonoid compounds reported to have antioxidative activity . Further, as reported on the relationship between the content of the active ingredient and the antioxidant activity, although there is a difference depending on the method of measuring the antioxidant activity and the kind of the radical, the total polyphenol content and / or the total flavonoid content is generally high It can be seen that the composition exhibits excellent antioxidative activity.

On the other hand, in the modern society, diseases caused by hyperglycemia such as diabetes are increasing due to changes in diet and lifestyle. Hyperglycemia is not only induced by ingesting foods with high glycemic index, but also caused by lack of exercise, overeating and / or excessive stress. Because hyperglycemia not only causes diabetes but also increases the risk of serious liver disease by causing cardiovascular disease and its complications or fatty liver, it is very important to maintain normal blood glucose status. In this regard, the polyphenols and / or flavonoids are also known to exhibit a hypoglycemic effect, so that a blood glucose lowering method using natural substances containing these substances at a high concentration can be sought.

Accordingly, the inventors of the present invention have made intensive efforts to produce a germinated barley tea containing an increased content of antioxidant or hypoglycemic active ingredient, and found that the germinated barley germinated 3-5 days after sowing was prepared at 180-250 ° C The present inventors completed the present invention by confirming that the hot water extract of green tea barley contains high content of polyphenols and flavonoids as antioxidative active ingredients.

An object of the present invention is to provide a method of harvesting roasted barley, And a second step of boiling the harvested germanium barley at 180 to 250 DEG C, wherein the antioxidative or hypoglycemic active ingredient content is increased.

Another object of the present invention is to provide a sprouts barley tea prepared by cutting the above ground portion of bud barley at 180 to 250 ° C.

It is still another object of the present invention to provide a method for producing an antioxidant or hypoglycemic active ingredient comprising extracting the germanium barley tea with a water-containing solvent.

Another object of the present invention is to provide a food composition for antioxidant, anti-inflammation or hypoglycemia, which contains as an active ingredient a germanium barley tea extract prepared by reducing the above-ground part of bud barley at 180-250 ° C.

Another object of the present invention is to provide a cosmetic composition for antioxidant or antiinflammation which comprises, as an active ingredient, a germanium barley tea extract prepared by reducing the above-ground part of bud barley at 180 to 250 ° C.

Another object of the present invention is to provide a pharmaceutical composition for antioxidant, antiinflammation or hypoglycemia, which comprises, as an active ingredient, a germanium barley tea extract prepared by reducing the above-ground part of bud of barley at 180 to 250 ° C.

In order to accomplish the above object, the present invention provides a method for producing soy sauce comprising: a first step of harvesting a ground portion of a bud barley; And a second step of boiling the harvested germanium barley at 180 to 250 DEG C, wherein the content of the antioxidant or hypoglycemic active ingredient is increased.

In the present invention, the term "sprouts barley" is also referred to as barley sprouts and barley sprouts, which means young barley leaves. As in the concrete examples of the present invention, barley seeds are evenly sprinkled on the soil using a box for seedling growing at room temperature, the soil is covered, the barley is grown while spraying water without drying, and sprouted for days to tens of days After this, the barley can be harvested and used. Preferably 3 to 5 days after sowing, can be used. The tea made from the bud barley harvested at this time has the highest content of the active ingredient, so that it can exhibit more excellent antioxidant or hypoglycemic activity. In the present invention, the above-mentioned portion of the bud barley ground can be purchased commercially, or can be harvested or cultivated in nature.

When the barley is grown from the barley seed to obtain the bud portion, the shoot barley can be grown at a temperature of 15 to 28 캜 after sprouting, more preferably at a temperature of 20 to 25 캜.

In the present invention, the term "above ground part " means a part of the foliage above the surface of the earth, centering on an indicator in the plant. That is, it refers to the upper surface of the ground excluding the roots of the bud barley, which may include leaves, stems, flowers, etc., but preferably refers to bud leaves of barley. In the case of 3 days to 5 days after sowing, since there are much more diverse physiologically active ingredients, especially antioxidant or hypoglycemic activity, in the above-ground part, it is preferable to collect them at this time.

In order to produce the germinated barley tea of the present invention having an increased antioxidant or hypoglycemic active ingredient content, it is preferred that the above ground portion of the harvested germinated barley is heated at 180 to 250 ° C. If the digestion process is carried out at a temperature lower than 180 ° C, the elution of the active ingredient of the barley barley may be reduced, and if carried out at a temperature exceeding 250 ° C, the barley leaf may burn or be damaged. Thus, in a specific example of the present invention, a sprout barley tea was prepared in a fermenter at 200 to 220 ° C for 10 to 20 minutes (Example 1).

In a specific example of the present invention, the hot-water extract of the sprout barley tea prepared from the sprout barley harvested at 3-5 days after sowing was compared with the hot-water extract of the dried sprout barley at the same time, (Table 1). ≪ tb > < TABLE > Furthermore, compared with the samples prepared at different times, the sprouts barley prepared from the sprout barley harvested at 3-5 days after sowing exhibited the highest content of antioxidant or hypoglycemic active ingredients polyphenols and flavonoids (Table 2) and showed the best antioxidative activity, for example, a radical scavenging activity (Tables 3 and 4).

Preferably, the method of manufacturing a germanium barley tea according to the present invention further comprises a third step of drying germinated barley in the second step and a fourth step of germinating the dried germanium barley at 80 to 120 ° C .

In another embodiment, the present invention provides a shoot barley tea prepared by cutting the above ground portion of bud barley at 180 to 250 ° C.

It is preferable that the sprout barley of the present invention has an increased content of antioxidant or hypoglycemic active ingredients through the roasting process and is prepared at 180 to 250 ° C, which is confirmed as optimal roasting conditions as described above. Furthermore, it is preferable that the above-mentioned shoot barley tea is prepared by using the barley harvested at 3 to 5 days at 18 to 26 ° C after sowing. The top part of the barley harvested at 3 ~ 5 days at 18 ~ 26 ℃ after sowing with the highest total polyphenol and total flavonoid content was cultured at 180 ~ 250 ℃, which was confirmed as the optimum sonication condition to maximize the content of active ingredient It contains an increased antioxidant or hypoglycemic active ingredient compared to the tea made from bud barley taken at different times.

In another aspect, the present invention provides a method for producing an antioxidant or hypoglycemic active ingredient, comprising extracting the germanium barley tea with a water-containing solvent.

The term "antioxidant or hypoglycemic active ingredient" of the present invention collectively refers to a component that particularly contributes to an antioxidative effect or a hypoglycemic effect among various components of the extract. As known in the art, the young leaves (barberry barley) extracts of barley or barley generally contain polyphenols and flavonoids known to have antioxidant or hypoglycemic activity. Accordingly, in the present invention, the active ingredient for antioxidant or hypoglycemic action may be polyphenol or flavonoid, but is not limited thereto, and includes, for example, a substance exhibiting antioxidative or hypoglycemic activity as a component of the germinated barley tea extract.

The term "water-containing solvent " of the present invention refers to water or a mixture of water and another solvent, which may be a mixture of water and an organic solvent such as an alcohol, an oil-in-water fermentation alcohol, or 100% water. Preferably, the extraction may be performed by using water as a solvent, and more preferably, it may be extracted with hot water for more effective antioxidation or extraction of the hypoglycemic active ingredient. The temperature of the hot water is not limited so long as it promotes the extraction of the active ingredient and does not destroy the active ingredient. In a specific embodiment of the present invention, water, in particular, hot water at 60 to 90 DEG C was used as the solvent.

Preferably, the antioxidant or hypoglycemic active ingredient may be a polyphenol or a flavonoid.

The term "polyphenol" in the present invention generically refers to a compound having a plurality of phenolic structural units, and includes mainly synthetic or semisynthetic organic compounds derived from natural products. The number and nature of the phenolic structures determine the unique physical, chemical and biological (metabolic, toxic and therapeutic) properties of the compounds. Polyphenols can be broadly classified as phenolic acids, flavonoids, stilbenes, and lignans. The name polyphenol is derived from "phenol" which refers to a chemical structure in which a Greek word "poly " means a plurality and an aromatic benzenoid (phenyl) ring is linked to a hydroxyl group contained in an alcohol compound .

In the present invention, the term "flavonoid" is also referred to as bioflavonoids and is also referred to as vitamin P as secondary metabolites of plants. According to the IUPAC nomenclature it is possible to obtain a compound of formula (I) which is derived from the structure of 2-phenylchromen-4-one (2-phenyl-1,4-benzopyrone) Flavones; Isoflavonoids derived from 3-phenylchromen-4-one (3-phenyl-1,4-benzopyrone) ; And neoflavonoids derived from the structure of 4-phenylcoumarine (4-phenyl-1,2-benzopyrone). All three flavonoids are compounds containing a ketone group. More broadly, the terms flavonoid and bioflavonoid include compounds that are specified as non-ketone polyhydroxypolyphenol compounds, more specifically flavonoids, flavan-3-ol (or catechins).

It is known in the context of the present invention that the polyphenols and / or flavonoids exhibit antioxidant or hypoglycemic effects from in vitro experiments. Although no specific correlation has been found, although there is a difference depending on the method of measuring antioxidant or hypoglycemic activity and the kinds of radicals used, the higher the amount of polyphenol and / or flavonoid in the composition, the better antioxidant or hypoglycemic activity ≪ / RTI >

More preferably, the antioxidant or hypoglycemic active ingredient may be, but is not limited to, saponin, rutonarin, bapticin, isoviticin, isoorientin, 3-O-peryllucinic acid or a combination thereof.

The term "saponin" in the present invention is flavonoid and flavone glucoside, which is broadly belonging to polyphenol. Also called 5-hydroxy-2- (4-hydroxyphenyl) -6- [3,4,5-trihydroidene-7-O-glucoside Yl) -7- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan-2-yl] oxychromen- (5-hydroxy-2- (4-hydroxyphenyl) -6- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan- hydroxymethyl) oxan-2-yl] oxychromen-4-one. And also has a formula of C27H30O15 and a molecular weight of 594.53 g / mol. It is found in Saponaria officinalis and Strongylodon macrobotrys, and imparts a characteristic jade color to flowers. For example, the yaffe contains an anthocyanin and saponin in a ratio of 1: 9. Saponin is dark yellow under weakly basic conditions and imparts a green tone to the flower. It is also present in the passion flower of Passiflora sp. Saponaretin and vitexin are produced by acid hydrolysis.

In another aspect, the present invention provides an antioxidant, anti-inflammatory, or hypoglycemic food composition comprising a bud root tea extract prepared by starving a bud portion of a bud barley at 180 to 250 ° C as an active ingredient. Preferably, the food composition is selected from the group consisting of a sprout barley tea prepared by harvesting sprout barley at 3 to 5 days at 18 to 26 캜 after sowing, which is a period of maximum content of the active ingredient to maximize antioxidant, anti- ≪ / RTI >

In the present invention, the term "antioxidative activity" means an action of inhibiting oxidation, and means an action of eliminating reactive oxygen species generated in the human body. The human body has a balance between prooxidant and antioxidant. When the balance is broken due to various factors and is deviated toward oxidation promotion, oxidative stress is induced, Resulting in cellular damage and pathological disease. Reactive oxygen species (ROS), which is a direct cause of such oxidative stress, is an unstable form of oxygen with unpaired electrons. It is composed of oxygen ions, free radicals and peroxides peroxides). It is unstable and highly reactive, easily reacts with biomaterials, damages cell lipid membranes, intracellular proteins and DNA, causing mutations, and irreversible damage to cells and tissues, resulting in cell death And further aging and various diseases. In addition, reactive oxygen species are produced with reactive nitrogen species (RNS) such as NO, HNO ₂ , and ONOO ^- , which are produced by the immune response of macrophage neutrophils and other immune cells during inflammatory reactions. The sprout barley tea extract of the present invention has an excellent antioxidative activity by exhibiting excellent radical scavenging ability by containing a large amount of the above-mentioned flavonoids.

The term "anti-inflammatory activity" in the present invention means an action that resists and resists inflammation. The inflammatory reaction is a very complicated process of regulation, and occurs to reduce the enhancement and damage of the in vivo restoration system. However, repeated inflammatory responses by tissue damage or regeneration can lead to excessive production of ROS and RNS in the cells involved, resulting in permanent genetic modification. Thus, ROS and RNS are involved in the inflammatory response that regulates the action of various cells in vivo. During the inflammation process, a large amount of proinflammatory cytokines, nitric oxide (NO) or prostaglandin E2 (PGE2) and the like are induced by inducible nitric oxide synthase (iNOS) and cyclooxygenase It is known to be produced by cyclooxygenase-2 (COX-2). Due to such inflammation inducing substances, various inflammatory diseases can be induced by inflammation, and since the sprout barley tea extract of the present invention contains a large amount of flavonoid compounds and exhibits an anti-inflammatory action, it is possible to prevent and improve inflammatory diseases Lt; / RTI >

In the present invention, the term "hypoglycemic activity" refers to an effect of lowering the blood glucose, which is a value indicating the glucose concentration in the blood. The human body preferably maintains the blood glucose level within a certain range in order to maintain the homeostasis. Hyperglycemia means an abnormally high blood glucose level. Hyperglycemia, which appears temporarily after a meal, is a natural phenomenon and is called physiological hyperglycemia. However, the increase in blood sugar level or persistence of hyperglycemia in the range beyond this is likely to be due to diabetes, progression to diabetes, or diabetes but not yet discovered. The US Food and Drug Administration (FDA) requires that normal blood glucose levels be less than 100 mg / dl fasting glucose and less than 140 mg / dl two hours after meals. Fasting blood glucose levels greater than or equal to 126 mg / dl and 2 hours Diabetes is diagnosed. WHO, on the other hand, defines fasting blood glucose below 110 mg / dl as the normal level. Even if it is less than the diagnosis of diabetes, persistence of a hyperglycemic state higher than normal may increase the risk of progressing to severe liver disease by inducing fatty liver, which may lead to cardiovascular disease and its complications. Glucagon, adrenaline, insulin, and thyroid hormones work in the body to maintain normal blood glucose levels. An abnormality in the secretion and / or activity of these hormones may cause hyperglycemia. Excessive intake of sugar, lack of exercise and / or stress also causes hyperglycemia. The sprout barley tea extract of the present invention is a flavonoid known to have a hypoglycemic effect (Food Chem. Toxicol. 2008, 46 (7): 2376-2383), polyphenol (J. Agric. 36): 8860-8865) and saponin (J. Enzyme Inhib. Med. Chem., 2009, 24 (3): 684-690) in a large amount to exhibit a hypoglycemic effect, And can have a preventive and / or ameliorative effect on diseases.

In the present invention, the term "extract" means a preparation in which a sample is squeezed with a suitable leaching solution and the leaching solution is concentrated by evaporation. Without being limited thereto, the extract solution, the diluted solution, A dried product to be obtained, a controlled preparation thereof or a purified product thereof. The extract of the present invention can be prepared by extracting with an extraction solvent or extracting with an extraction solvent and fractionating the extract with a fraction solvent. The extraction solvent may be, but is not limited to, water, an organic solvent, or a mixed solvent thereof. The organic solvent may be an alcohol having 1 to 4 carbon atoms, a polar solvent such as ethyl acetate or acetone, hexane or dichloromethane Non-polar solvents or mixed solvents thereof may be used. Water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be preferably used. Specifically, a solvent having a volume of about 2 to 50 times, preferably about 5 to 20 times the dry weight may be added and extracted at a temperature of 20 to 100 ° C, preferably room temperature, for several hours to several days. Non-limiting examples of extraction methods include hot water extraction, cold extraction, reflux cooling extraction, and ultrasonic extraction. In a specific embodiment of the present invention, an extract was prepared using water as the solvent, particularly hot water at 60 to 90 ° C. The extraction was carried out for about 12 hours with stirring at room temperature.

The term "prevent" in the present invention means a disease caused by metabolic disorders comprising the extract of sprout barley tea according to the present invention or a pharmaceutical composition for the prevention or treatment of cardiovascular diseases, Or any action that inhibits or delays the onset of cardiovascular disease.

The term "treatment" in the present invention means treatment of a disease caused by metabolic disorders comprising the extract of sprout barley tea according to the present invention or a pharmaceutical composition for the prevention or treatment of cardiovascular diseases, Or any action that improves or alters the symptoms of cardiovascular disease.

Specifically, the extract of the present invention has antioxidative, anti-inflammatory and hypoglycemic effects and can be added to the food composition for the purpose of antioxidation, anti-inflammation or hypoglycemia.

When the sprout barley tea extract of the present invention is used as a food additive, the extract may be added as it is or may be used together with other foods or ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use. The food additives are intended to improve the appearance, flavor, texture, or shelf life and are intended to be added intentionally to food. The quality of the food is improved to improve preservability or palatability, It is meant to be used for the purpose of promoting value. This may be a substance used in food production, processing, or preservation as defined in Article 2 (2) of the Food Sanitation Act, by addition, mixing, infiltration, or other methods to food.

The food composition of the present invention may include all foods having a conventional meaning, and may be mixed with terms known in the art such as functional foods, health functional foods, and the like.

The term "functional food" in the present invention means a food prepared and processed by using a raw material or ingredient having functionality useful to the human body according to Law No. 6727 on health functional foods, and " And function of the nutrient for the purpose of obtaining a beneficial effect in health use such as controlling the nutrient or physiological action.

The term "health functional food" of the present invention refers to a food prepared by processing a specific ingredient as a raw material for the purpose of health assisting or by extracting, concentrating, refining, mixing, or the like a specific ingredient contained in a food raw material, Refers to a food which is designed and processed so that the body control function such as bio-defense, regulation of biorhythm, prevention and recovery of disease and the like can be sufficiently exhibited to the living body by the above components. Recovery, and so on.

There is no limitation on the kind of food in which the composition of the present invention can be used. In addition, the composition comprising the extract of sprout barley tea according to the present invention as an active ingredient can be prepared by mixing other suitable auxiliary ingredients that can be contained in food and known additives, according to the selection of a person skilled in the art. Examples of foods that can be added include dairy products, such as meat, sausage, bread, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Vitamin complex, and the like, and can be prepared by adding to the juice, tea, jelly, and juice prepared from the extract of the present invention as a main component. It also includes foods used as feed for animals.

Examples of foods that can be used in the present invention include special nutritional foods such as crude oil, milk, baby food, meat products, fish meat products, tofu, mushrooms, noodles such as ramen noodles, (Such as soy sauce, doenjang, kochujang, mixed potatoes), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickles ), Beverages (such as fruit, vegetable beverages, beverages, fermented beverages, etc.), and natural seasonings (eg, ramen soup, etc.).

In addition to the above, the food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, , A carbonating agent used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. The food may also be prepared in the form of tablets, granules, powders, capsules, solutions in liquid form, and the like according to known production methods. There are no particular restrictions on the other ingredients other than those containing the extract of the present invention as an active ingredient, and various common flavoring agents or natural carbohydrates may be added as an additional ingredient.

In another aspect, the present invention provides a cosmetic composition for antioxidant or anti-inflammation, which contains, as an active ingredient, a germanium barley tea extract prepared by reducing the above-ground part of bud of barley at 180 to 250 ° C.

The above-mentioned germanium barley extract and its antioxidant or anti-inflammatory activity are as described above. Preferably, the cosmetic composition contains a sprout barley tea extract prepared from sprout barley harvested at 3 to 5 days at 18 to 26 ° C after sowing, which is a period of maximally containing the active ingredient to maximize antioxidant or anti-inflammatory effect can do.

Specifically, the extract of the present invention has antioxidative and antiinflammatory properties and can be added to cosmetic compositions for the purpose of antioxidant or antiinflammation.

The cosmetic composition according to the present invention can be manufactured into various formulations used in the art. Examples of cosmetic formulations may be formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays , But is not limited thereto. More specifically, the cosmetic composition of the present invention can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder, And any other cosmetic composition having a skin adhesive formulation may be used as the cosmetic preparation of the present invention.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a chlorofluorohydrocarbon, Propane, such as butane or dimethyl ether.

When the formulation of the present invention is a solution or a milky lotion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl Aliphatic alcohols, fatty acid glycerides, fatty acid diethanol amides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

In another aspect, the present invention relates to a method for producing an anti-oxidant, anti-inflammatory, anti-inflammatory, anti-inflammatory, anti-inflammatory, anti- Or a pharmaceutical composition for lowering blood glucose levels. Preferably, the pharmaceutical composition comprises, in order to maximize antioxidant, anti-inflammatory or hypoglycemic effect, a sprout barley harvested at 3 to 5 days at 18 to 26 ° C after sowing, Tea extract may be included.

The antioxidant activity is as described above, and thus can be included in a pharmaceutical composition for the purpose of antioxidation, and can have an effect of preventing or treating a disease caused by active oxygen. The diseases caused by the active oxygen include, but are not limited to, degenerative neurological diseases including arteriosclerosis, Lou Gehrig's disease, Parkinson's disease, Alzheimer's disease, proximal axillary sclerosis and Huntington's disease, myocardial infarction, angina pectoris, coronary artery disease, May include various diseases such as cardiovascular diseases including diseases, ischemic brain diseases including stroke, digestive system diseases including diabetes, gastritis and gastric cancer, cancer, leukemia, aging, rheumatoid arthritis, hepatitis, atopic dermatitis, Preferably aging caused by active oxygen.

In addition, the anti-inflammatory activity is as described above and can be included in a pharmaceutical composition for the purpose of anti-inflammation, that is, for the prophylaxis or treatment of inflammatory diseases. The inflammatory diseases include allergic diseases including allergic asthma, allergic rhinitis, allergic mucositis, urticaria, and anaphylax, although it is not limited thereto. Arthritis, atopic dermatitis, psoriasis, asthma, multiple sclerosis, ssRNA and dsRNA viral infection, sepsis, multiple chondritis, scleroderma, including systemic sclerosis, dermatomyositis and inclusion body myositis , Eczema, gout, periodontal disease, Behcet's syndrome, edema, vasculitis, Kawasaki disease, diabetic retinitis, autoimmune pancreatitis, vasculitis, glomerulonephritis, acute and chronic bronchitis, and influenza infection.

In addition, the above hypoglycemic activity is as described above, and thus can be included in a pharmaceutical composition for the purpose of lowering blood glucose, that is, for the prevention or treatment of diseases caused by hyperglycemia. The diseases caused by hyperglycemia are collectively referred to as diabetes, which shows a high blood sugar level above a certain level, and diseases that can be caused by persistence of a blood sugar level to a high level of normal or higher. Examples of such diseases include cardiovascular diseases such as angina, myocardial infarction, Cerebrovascular disease, other vascular diseases such as hypertension, liver disease such as fatty liver, or retinopathy.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable" of the present invention means that it exhibits properties that are not toxic to the cells or humans exposed to the composition. Such carriers may be used without limitation as long as they are known in the art such as buffers, preservatives, wetting agents, solubilizers, isotonic agents, stabilizers, bases, excipients and lubricants.

In addition, the pharmaceutical composition of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method have. Furthermore, it can be used in the form of an external preparation for skin in the form of ointments, lotions, spray agents, patches, creams, powders, suspensions, gels or gels. Examples of carriers, excipients and diluents that can be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate ), Sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use may include various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc. in addition to water and liquid paraffin, which are simple diluents commonly used in suspension, liquid solutions, emulsions and syrups have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

Meanwhile, the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The term "administering" of the present invention means introducing a predetermined substance into an individual by an appropriate method, and the administration route of the composition of the present invention can be administered through any ordinary route as long as it can reach the target tissue. But are not limited to, intraperitoneal, intravenous, intramuscular, subcutaneous, intradermal, oral, topical, intranasal, intrathecal, rectal.

The term "individual" refers to all animals such as mice, mice, livestock, and the like, including humans that have developed or are capable of developing a disease or inflammatory disease caused by active oxygen. Preferably, it may be a mammal, including a human. Since the composition of the present invention exhibits antioxidant, anti-inflammatory or hypoglycemic activity, the composition can be administered to an individual to effectively prevent or treat a disease caused by active oxygen, an inflammatory disease or a disease caused by hyperglycemia. The composition of the present invention may be administered in combination with a therapeutic agent for diseases or inflammatory diseases caused by existing active oxygen.

The term "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment and not causing side effects, and the effective dose level is determined by the patient's sex, Including, but not limited to, medicaments and other medical fields that are used in combination, or in combination with, or in combination with, a pharmaceutically acceptable carrier, excipient, condition, type of condition, severity, activity of the drug, sensitivity to the drug, Can be readily determined by those skilled in the art according to known factors. Generally, the active substance may be administered at a dose of from about 0.01 mg / kg / day to 1000 mg / kg / day. For oral administration, a range of 50 to 500 mg / kg may be suitable. The administration may be carried out once per day, or divided into several doses.

The sprout barley tea extract of the present invention can be used safely and usefully as a food composition, a cosmetic composition, or a pharmaceutical composition because it has fewer side effects than a synthetic compound in general because it uses a natural material as a raw material.

Since the sprout barley tea of the present invention is manufactured from edible plants, there is little concern about side effects such as toxicity, and it is provided in the form of tea, so that it can be easily ingested without any feeling of rejection, and the maximum content of polyphenols and flavonoids And contains an antioxidant active ingredient in an increased content. Therefore, it can be effectively used for the prevention or treatment of diseases caused by reactive oxygen species or inflammatory diseases. Since the polyphenols and / or flavonoids have an effect of lowering the blood sugar level, the sprouts barley prepared from the sprout barley harvested at the time when the polyphenols and / or flavonoids are contained at the maximum level contains the hypoglycemic active ingredient, Can be usefully used for the prevention or treatment of diseases caused.

Fig. 1 is a diagram showing the shoot barley (top) and its water extract (bottom) harvested at each harvesting time indicated after sowing. Fig.
FIG. 2 is a graph showing the total polyphenol and total flavonoid contents of the hot-water extract of green tea barley according to harvesting time. FIG. As a control, green tea hot water extract was used.
FIG. 3 is a graph showing antioxidant activity of the extract of hot pepper barley tea according to harvesting time. FIG. Antioxidant activity was measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging activity. Extracts were used.
FIG. 4 is a graph showing the liquid chromatogram of saponin, which is the main antioxidant component, of the hot water extract of green tea barley according to the harvesting time.
FIG. 5 is a graph showing the cytotoxicity of the hot-water extract of shoot barley tea according to harvesting time. FIG.
FIG. 6 is a graph showing the activity of suppressing nitrogen monoxide production of the extract of hot pepper barley tea according to harvesting time. FIG.
7 is a graph showing the activity of suppressing nitrogen monoxide formation according to the treatment concentration of saponin.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

Example  One: Increased  Preparation of germinated barley tea containing the antioxidant or hypoglycemic active ingredient content

The present inventors have disclosed that the extract of bud from barley extract exhibits antioxidative activity and contains polyphenolic compound such as saponin as an active ingredient (Korean Patent No. 10-1174497). In the present invention, in order to extract the antioxidant or hypoglycemic active ingredient from the above-mentioned germanium barley, the obtained germanium barley was sown at high temperature to prepare a hot water extract, and the saponin content was confirmed.

Specifically, the seedling box is used at room temperature to coat the barley seeds on the soil or free soil, cover the soil or vinyl, and maintain the cultivation temperature at 18 to 22 ° C. Spray water with a shower so that the sown seeds do not dry, I raised barley. After the sowing, the ground part of the bud barley was harvested 3-5 days after the sowing, and the living body was harvested and washed at 200 to 220 ° C for 10 to 20 minutes using a cast iron pot. The dried germanium barley was dried at room temperature and then re-roasted at 80 to 120 ° C for 5 to 10 minutes using a cast iron pot to prepare a germanium barley tea. The control group was sampled at the same time and prepared by simple drying. 1 g of each of the prepared germanium barley tea and simple dried germane barley was taken in a tube, and 10 ml of water at 60 to 90 ° C was added thereto, followed by stirring at room temperature for about 12 hours. After the extraction, the leaves were filtered with a paper filter and further filtered using a 0.45 μm syringe filter to prepare a hot-water extract of sprout barley and simple dried sprout barley.

The content of saponin in the hot-water extract was measured at 335 nm using a Waters ACQUITY BEH C18 column (particle size 1.7 μm, 2.1 × 100 mm, Waters) and ultrahigh pressure liquid chromatography (UPLC, Waters, USA) Respectively. Two solvents were used at different ratios in the mobile phase: 0.1% TFA (trifluoro acetate, Sigma, USA) dissolved in tertiary distilled water as the solvent A and 100% acetonitrile (Sigma, USA) 0.5 ml flow rate. Standard saponin was prepared by concentration (10, 50, and 100 μg / ml), and a calibration curves were prepared and compared with the above calibration curves. The Saponarin standard product was the method described in the prior patent (Korean Patent No. 10-1174497). The saponin content of the barley and simple dried barley hot water extracts obtained by the above method is shown in Table 1 below.

* As shown in the above Table 1, when the dried barley was prepared by carving, the content of saponin as an active ingredient in the hot-water extract was twice or more as compared with the sample prepared by simply drying the barley taken at the same time Respectively. These results indicate that the content of antioxidant or hypoglycemic active component in the hot-water extract of the present invention can be increased through the process of swelling at a high temperature. Therefore, it was confirmed that the content of antioxidant or hypoglycemic active ingredient can be increased by preparing tea from barley at high temperature.

Example  2: Preparation of sprouts barley tea according to harvesting time

Antioxidant or hypoglycemic agents To determine the harvesting time of the barley containing the active ingredients at the maximum, barley seeds were sprayed on the soil or free soil using a seedling box at room temperature, covered with soil or vinyl, and cultivated at a temperature of 18 to 22 ° C And sprout barley was grown while spraying with water to prevent sown seeds from drying. 3-5 days, 6-7 days and 10 days after sowing, the upper part of the shoot barley was harvested, and the living body was harvested, washed and then spun at 200-220 ° C for 10-20 minutes using a cast iron pot. The dried germanium barley was dried at room temperature and then re-roasted at 80 to 120 ° C for 5 to 10 minutes using a cast iron pot to prepare a germanium barley tea. FIG. 1 shows the images of the shoot barley tea and the water extracts prepared at the respective harvesting times.

Example  3: Sprout barley tea according to harvesting time Heat number  Measure total polyphenol and total flavonoid content of extract

3.1. Total polyphenol content measurement

1 g of the sprouts barley tea prepared according to the harvesting time according to the above Example 2 was taken in a tube, 10 ml of water at 60 to 90 ° C was added, and the mixture was stirred and extracted at room temperature for about 12 hours. After the extraction, the leaves were filtered with a paper filter and further filtered using a 0.45 μm syringe filter to prepare a germinated barley tea extract. 100 μl of sprout barley tea extract prepared by filtration was put into a test tube, and 500 ml of a 0.2 N folin-ciocalteu's phenol reagent solution was added thereto. 1.3 ml of 10% sodium hydrogencarbonate (Na2CO3) solution and 6 ml of distilled water were added. After reacting at room temperature for 2 hours, the absorbance at 765 nm was measured. To determine total polyphenol contents, quantitative curves were prepared using various concentrations of gallic acid as a control group, and the total polyphenol contents were calculated by substituting the measured values from the extracts of ginseng barley tea.

3.2. Total flavonoid content measurement

10 ml of the germinated barley tea extract prepared according to the same method as in 3.1. Above was prepared in a flask. 1 ml of 1N NaOH solution and 10 ml of diethyl glycol were added. Quercetin (quercetin) was prepared as a control group for total flavonoid content and treated in the same manner as the sample. The sample or the control, the mixture of NaOH and diethylglycol was allowed to react at room temperature for 1 hour, and the absorbance at 420 nm was measured to calculate the total flavonoid content.

* The total polyphenol and total flavonoid content of the germinated barley tea extract according to the harvesting time measured in Example 3 are shown in Table 2 and FIG.

As shown in the above Table 2, the highest value of total polyphenol and total flavonoid content in the extract of sprout barley tea prepared from the sprout barley sampled 3 to 5 days after sowing among the sprout barley extracts of the present invention And 1.5 times total polyphenol and 1.2 times total flavonoid content as compared with the control green tea. From these results, it was confirmed that the extract of sprouts barley prepared with the bud roe harvested 3-5 days after sowing contained the highest level of antioxidant or hypoglycemic active ingredient.

Example  4: Sprouts barley tea Heat number  Antioxidant activity of extract

To determine the antioxidative activity of the germinated barley tea extract according to the harvesting time according to Example 2, DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid).

4.1. DPPH  Radical scavenging activity

DPPH exhibits strong absorption at 517 nm due to an odd number of stable radical electrons that it possesses. On the other hand, when it reacts with an electron donor which provides electrons to hydrogen such as polyphenol, electrons receive hydrogen radicals, Forms a radical and becomes a molecule in a stable form, thus decreasing the absorbance at this wavelength. At this time, the donated electrons were irreversibly bound, and the purple color of DPPH gradually became thinner and the absorbance decreased in proportion to the number. After addition of 1 ml of sample and 1 ml of 0.15 mM DPPH (Sigma Co., USA) to a 12-well plate, the mixture was reacted at 30 ° C for 5 minutes in a shaking incubator, and further reacted at room temperature for 30 minutes. Then, using a spectrophotometer, 517 nm absorbance was measured. The antioxidant activity of the sample, that is, the reducing power, was expressed as 50% inhibitory concentration (IC ₅₀ ). The values calculated using the following equations are shown in Table 3. < tb >< TABLE >

DPPH antioxidant effect (%) = (absorbance _blank - absorbance _sample ) / absorbance _blank × 100

As shown in the above Table 3, among the extracts of sprout barley tea according to the present invention, the sprout barley tea extract obtained from 3-5 days after sowing, The DPPH radical scavenging activity, that is, the high antioxidant activity, was shown to be twice as high as that of the control green tea extract.

4.2. ABTS  Radical scavenging activity

ABTS is a method for measuring selective radical scavenging activity on hydroxy radicals. The ABTS exhibits strong absorption at 734 nm due to the odd number of stable radical electrons it possesses, while hydrogen such as polyphenol Reacts with an electron donor that provides electrons, the electrons receive hydrogen radicals and turn into stable molecules, thus the solution changes from blue to colorless and the absorbance at that wavelength decreases. At this time, the donated electrons were irreversibly bound, and the blue color of ABTS gradually decreased and the absorbance decreased in proportion to the number. A 12-well plate was reacted with 7.4 mM ABTS (Sigma Co., USA) and 2.6 mM potassium persulfate in a dark room at room temperature for about 12 hours to form a radical. Then, 50 μl of the sample and the ABTS solution 950 μl were mixed and reacted in a dark room for 10 minutes. Absorbance was measured at 734 nm using a spectrophotometer. As a control, trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was used instead of the sample. The antioxidant activity of the sample, that is, the reducing power, was expressed as 50% inhibitory concentration (IC ₅₀ ). The values calculated using the following equations are shown in Table 4. < tb >< TABLE >

ABTS antioxidant effect (%) = ((E (ABTS ⁺ ) - E (control) / ABTS)

As shown in the above Table 4, among the extracts of sprout barley tea according to the present invention, the sprout barley tea extract prepared from the bud 3-5 days after sowing, The antioxidant activity of the ABTS radical scavenging activity was significantly higher than that of the green tea extract of the control group.

As shown in Tables 2 to 4, the germinated barley tea extract prepared from the germinated barley 3-5 days after sowing was prepared from the germinated barley germ or the antioxidant activity of green tea extract , 1.5 to 2.4 times, and 1.1 to 1.3 times, respectively, of the polyphenols and flavonoids, respectively, and thus showed remarkably improved antioxidative activity. For example, sprouts barley prepared with 3-5 days after sowing showed DPPH and ABTS inhibitory activity improved by 1.8-2.5 times and 1.2-1.6 times, respectively.

Example  5: Extraction time of sprout barley tea extract Saponin  Quantitative analysis of compounds

The contents of the main antioxidant or saponin, which is an active ingredient for hypoglycemic action, in the extract of sprouts barley according to the harvesting time according to the above Example 2 were confirmed. The specific saponin content was analyzed by the method described in Example 1, and the results are shown in Table 5 below.

Example  6: Cytotoxicity test of the extract of barley tea extract

6.1. Cell culture

Raw 264.7 macrophages (Korean Cell Line Bank, Korea) were purchased and cultured in DMEM supplemented with 10% FBS (fetal bovine serum) and 1% antibiotics (100 U / ml penicillin and 0.1 mg / ml streptomycin) modified eagle medium) at 37 ° C with 5% CO ₂ (95% air).

6.2. Cytotoxicity measurement

The cytotoxicity of the extract of the bud root tea was confirmed by the principle that MTT is reduced to formazan in mitochondria of cells. The compositions containing 5 concentrations of each of the three extracts of sprout barley tea obtained according to the above Example 2 were treated with Raw 264.7 macrophages and analyzed for cytotoxicity by adding MTT solution. Specifically, the MTT solution was added and reacted at 37 ° C for 4 hours. The resulting formazan was dissolved by adding DMSO and the absorbance was measured at 570 nm. The results are shown in Fig.

As shown in FIG. 5, it was confirmed that the extract of sprouts barley prepared from each harvesting time showed a survival rate of about 75% or more up to 500 ppm for Raw 264.7 cells and did not show any cytotoxicity .

Example  7: Nitrogen Removal Activity of Sprout Barley Tea Extract

1.2 × 10 ⁴ cells were cultured in 96-well plates (Raw 264.7 cells) and cultured for 24 hours. LPS (lipopolysaccharide) was prepared at a concentration of 10 μg / ml and treated with 1 μl each well to induce inflammation And the amount of nitric oxide (NO) produced by LPS was measured using a nitrate assay kit (Cyman chemical, US). The results are shown in FIG.

As shown in Fig. 6, in the cells treated with the hot-water extract of sprouts barley prepared from the sprout barley harvested at 3-5 days after sowing compared with the hot-water extract of the sprouts barley prepared at different times, And showed nitrogen production inhibitory activity. In other words, the inflammation reaction induced by the addition of LPS significantly increased the production of nitrogen monoxide and decreased the production of nitrogen monoxide when treated with the extract of sprout barley tea. Especially, it was remarkably decreased by hot water extract of sprouts barley prepared from 3-5 days after sowing. Therefore, the highest value of the antioxidant active ingredient was obtained from the sprouts barley harvested at 3-5 days after sowing And the like.

In order to support this, the inhibitory effect on the production of nitrogen monoxide induced by the inflammatory reaction according to the treatment concentration of saponin, which is a typical antioxidative active ingredient contained in the bud barley tea, was confirmed, and the results are shown in FIG.

As shown in FIG. 7, it was confirmed that the effect of inhibiting the formation of nitrogen monoxide was remarkably increased as the concentration of saponin treatment was increased. As can be seen from the results shown in FIG. 6, The highest content of ginseng barley tea hot water extract prepared from 3-5 days after ginseng barley supplementation contains saponin and other antioxidant active ingredients.

Example  8: Glycoprotein activity assay of shoot extract of barley tea

In order to confirm the hypoglycemic effect of the germinated barley tea extract according to the harvesting time according to Example 2, the inhibitory activity against alpha-glycosidase and alpha-amylase, enzymes involved in blood glucose lowering, was confirmed. Specifically, the alpha-glucosidase activity assay was carried out by hydrolysis with alpha-glucosidase using p-nitrophenyl-alpha-D-glucopyranoside as a substrate (Bioorg. Med. Chem. Lett. 2005, 15, 5514-5516). ≪ / RTI > Alpha-glycosidase used in this experiment was extracted from yeast (baker's yeast). It was dissolved in phosphate buffer (0.07 M, pH 6.8) at a concentration of 0.5 unit / Respectively. Next, the substrate, p-nitrophenyl-alpha-D-glycosidezate, was dissolved in phosphate buffer to a concentration of 100 mM to give a concentration of 5 mM, and the hot-water extract of the prepared shoot barley tea was treated at the same concentration After incubation at 37 ° C for 20 minutes, the absorbance was measured at 405 nm to calculate the inhibition rate of alpha-glucosidase inhibitory activity.

The activity of alpha-amylase using the reducing sugar assay was determined by measuring the amount of the substrate hydrolyzed by the alpha-amylase enzyme using 1% starch as a substrate using the DNS reagent (1% dinitrosalicylic acid, Amylase inhibitory activity was confirmed by measuring the change in absorbance by adding 12% potassium sodium tartrate in 0.4 M NaOH (Bioorg. Med. Chem. Lett. 2005, 15, 5514-5516). Specifically, a 1% starch solution dissolved in a buffer (Acetate buffer, 0.05 M, pH 4.5) as a substrate was pre-treated at 37 ° C for 10 minutes, and then 100 μl was quantified in a tube. The enzyme, alpha-amylase, was prepared in the buffer solution at a concentration of 0.25 unit / ml, and then 100 μl was quantified. The hot-water extract of the prepared sprout barley tea was treated at the same concentration, , And 300 [mu] L of a DNS reagent as a coloring reagent was added. Thereafter, the mixture was reacted by shaking at 100 ° C for 10 minutes in water, then cooled, and the absorbance at 540 nm was measured to calculate the inhibition rate of α-amylase. IC ₅₀ values representing 50% of the inhibition rate were also determined and are shown in Table 6 together with the above-mentioned inhibition rate of alpha-glucosides.

As shown in Table 6, the blood glucose lowering effect of the hot-water extract of the germinated barley tea harvested at the growing stage according to the present invention was examined. As a result, it was found that the germinated barley germinated by the germinated barley harvested about 3-5 days after the barley- (IC ₅₀ ) of 119.4 ± 9.2 and 254.2 ± 11.5 against glucosidase and alpha-amylase, respectively. Therefore, the hot-water extract of sprouts barley tea harvested at 3-5 days after sowing Showed excellent alpha-glucosidase and alpha-amylase inhibitory activity.

A: Absorbance value at 405 and 540 nm of the inhibitor added

B: absorbance value at 405 and 540 nm of no addition of inhibitor

Claims (8)

A first step of harvesting the above-ground portion of the shoot barley grown on days 3 to 5 grown at 18 to 26 ° C after sowing; And
And a second step of boiling the harvested germanium barley at 180 to 250 DEG C, wherein the content of the antioxidant or hypoglycemic active ingredient is increased.
The method according to claim 1,
A third step of drying the germinated barley in the second step and a fourth step of germinating the dried germinated barley at 80 to 120 ° C. Gt;
A sprout barley tea which is grown at 18 to 26 ° C after sowing and is prepared by cutting the above ground portion of the barley harvested at 3 to 5 days at 180 to 250 ° C.
A method for producing an antioxidant or hypoglycemic active ingredient, which comprises the step of extracting the sprout barley tea according to claim 1 or 2 or the sprout barley tea according to claim 3 with a water-containing solvent.
5. The method of claim 4,
Wherein the extract is a hot-water extraction.
5. The method of claim 4,
Wherein the antioxidant or hypoglycemic active ingredient is a polyphenol or a flavonoid.
5. The method of claim 4,
Wherein the antioxidant or hypoglycemic active ingredient is selected from the group consisting of saponin, rutonarin, bapticin, isoviticin, isoorientin and 3-O-perylrhicinic acid. .
An antioxidant, an anti-inflammatory or hypoglycemic food containing the germinated barley tea extract which is cultivated at 18 to 26 ° C after sowing and the ground portion of the germinated barley harvested at 3 to 5 days at 180 to 250 ° C, Composition.

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