KR101125224B1 - A composition for skin anti-aging comprising extracts or fractions of Eremochloa ophiuroides as an active ingredient - Google Patents

Abstract

The present invention is sentigrass ( Eremochloa) ophiuroides ) extract, a fraction thereof or an anti-aging composition containing an active fraction separated from the fraction as an active ingredient, more specifically, a centipedes extract, a fraction thereof or an active fraction separated from the fraction is a skin aging Since it is excellent in antioxidant activity that suppresses the oxidative stress that causes the skin can be usefully used as an anti-aging composition.

Centipedes (Eremochloa ophiuroides), antioxidant, DPPH, anti-aging

Description

A composition for skin anti-aging comprising extracts or fractions of Eremochloa ophiuroides as an active ingredient}

The present invention is sentigrass ( Eremochloa) ophiuroides ) relates to a skin anti-aging composition containing an extract or a fraction thereof as an active ingredient.

All aerobic organisms, including humans, survive through energy metabolism using oxygen (O ₂ ). However, when oxygen in the body is subjected to various physical, chemical, and biological stresses, harmful activities such as superoxide anion radical (O ₂ −), hydrogen peroxide (H ₂ O ₂ ), and hydroxy radicals (OH) It turns into an active oxygen species.

Free radicals, which are generated as a result of normal metabolic processes, are highly reactive, causing non-selective and irreversible destruction of cell components such as lipids, proteins, sugars, or DNA, resulting in oxidative stress in cells or tissues. As well as cardiovascular diseases such as stroke and atherosclerosis, chronic inflammatory diseases such as rheumatism, respiratory diseases or autoimmune diseases (Halliwellt, B. et al., Biochem. J , 219, 1-14, 1984; Freeman, BA et al., Lab Invest , 47, 412-426, 1982; Ames, BN, Science , 221, 1256-1264, 1983; Fridovich, I., Arch . Biochem. Biophys ., 247, 1-11, 1986; Vishwanath, MS, Nutrition in Clinical Practice , 10, 19-25, 1995), these oxidative damages accumulate over time leading to aging and death.

Recently, the theory that aging and geriatric diseases are caused by reactive oxygen species has been acknowledged, the development of antioxidants known as substances that can control the active oxygen species has been actively researched, superoxide dismutase (SOD) Antioxidative enzymes such as peroxidase, catalase, glutathione peroxidase and tocopherol, ascorbate, carotenoid and glutathione Much research has been done on antioxidants (Chang, SS, et. al ., J. Food Sic . 42: 1102, 1977; Hammerschmidt, PA et al., J. Food Sic . 43: 556, 1964), synthetic antioxidants such as 2,6-di-tert-butyl-4-hydroxyanisole (BHA) have been developed. Used in food field (Kitahara, K., et al ., Chem Pharm . Bull . 40: 2208, 1992; Hatano, T., Natural Medicines 49: 357, 1995; Masaki, H., et al ., Biol . Pharm . Bull. 18: 162, 1995).

However, consumer avoidance of synthetic antioxidants and carcinogenicity have been reported in animal experiments in which large amounts of synthetic antioxidants have been administered (Branen, AL, JAOCS 52:59, 1975). The use of synthetic antioxidants is increasingly limited. As such, there is an urgent need for the development of natural antioxidants that are both effective and safer.

Sentipedgrass ( Eremochloa ophiuroides ) is a slow growing turbulent turf, a perennial grass that crawls and forms turf. Poaceae belongs to the family, the scientific name is Eremochloa ophiuroides (Munro) Hack. It is mainly distributed in China, East Asia, Indochina, the northeastern United States, Meso America, and the Caribbean, and is widely adapted to various soil conditions, but is especially resistant to moist, low acidic soils and low-quality sandy soils.

As a morphological feature, the leaves are 15-30 mm long, 2-4 mm wide, flat with a mid vein, hairless except for the collar part, and the leaf ends are round. The stem has a very firm sheath, and the roots are thin branched stolons. The flower part has one raceme of about 3 to 5 inches, which has a purple, slightly flat, small spikelet arranged in two rows. Have Its main use is as a lawn or turf grass and as a forage due to its high leafiness and good taste.

Therefore, the present inventors, while studying natural plant extracts having excellent antioxidant activity and skin aging inhibitory activity, the extract extracted from Sentipedgrass, fractions thereof and active fractions separated from the fractions have an excellent antioxidant activity composition for preventing skin aging The present invention has been completed by revealing that it can be usefully used.

The object of the present invention is Centipedgrass ( Eremochloa) ophiuroides ) It is to provide a skin anti-aging composition containing an extract or a fraction thereof as an active ingredient.

In order to achieve the above object, the present invention provides a skin composition for preventing skin aging containing centipede glass extract, a fraction thereof or an active fraction separated from the fraction as an active ingredient.

In another aspect, the present invention provides a pharmaceutical composition for preventing skin aging containing Centipedgrass extract, a fraction thereof or an active fraction isolated from the fraction as an active ingredient.

In addition, the present invention provides a skin anti-aging health food containing the centifed glass extract, a fraction thereof or an active fraction isolated from the fraction as an active ingredient.

Centipedgrass extract, fractions thereof, or active fractions isolated from the fractions can be usefully used as an active ingredient of the skin anti-aging composition because they have antioxidant activity that inhibits oxidative stress causing skin aging.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for preventing skin aging containing centipede glass extract, a fraction thereof or an active fraction separated from the fraction as an active ingredient.

The centipedes grass extract is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:

1) extracting by adding an extracting solvent to centipede glass;

2) cooling the extract of step 1) and filtering; And

3) concentrating the filtered extract of step 2) under reduced pressure.

In the above method, the centipedes of step 1) can be used without limitation, such as those grown or commercially available. The centifed grass is preferably used as a leaf, but is not limited thereto.

The extraction solvent is preferably water, alcohol or a mixture thereof. As the alcohol, C ₁ to C ₄ lower alcohols are preferably used, and as the lower alcohols, ethanol or methanol is preferably used. As the extraction method, it is preferable to use shaking extraction or reflux extraction, but not always limited thereto. The extraction solvent is preferably extracted by adding 2 to 10 times the amount to the centipedes glass, but is not limited thereto. Extraction temperature is preferably 50 to 100 ℃ but is not limited thereto. In addition, the extraction time is preferably 2 to 5 days, but is not limited thereto. In addition, the extraction number is preferably one to three times, but is not limited thereto.

In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but it is not limited thereto. In addition, the drying is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

The fraction of the centifed glass extract or the active fraction separated from the fraction is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:

1) preparing an ethyl acetate fraction obtained by further dissolving the centifed glass extract in water and then performing a system fractionation in the order of n-hexane and ethyl acetate; And

2) preparing an active fraction by subjecting the fraction of step 1) to column chromatography.

delete

In the above method, the column chromatography of step 2) is an active fraction by performing column chromatography using a filler selected from the group consisting of silica gel, Sephadex, RP-18, polyamide, Toyopearl and XAD resin. Can be isolated and purified. Column chromatography can be carried out several times by selecting an appropriate filler as necessary.

We adsorbed the ethyl acetate fractions of centifedgrass to Flex-Colum ^™ (3 cm × 25 cm) and Toyoperarl HW-40 (75 μm) column chromatography and dissolved 20, 50 or 70% methanol. Using ethyl acetate-20% methanol, ethyl acetate-50% methanol and ethyl acetate-70% methanol were obtained.

The centipedes grass extract preferably includes any one or more of compounds selected from the group consisting of compounds represented by the following Chemical Formulas 1 to 8, but is not limited thereto.

The active fraction separated from the fraction of the centipedes grass extract preferably includes any one or more of compounds selected from the group consisting of compounds represented by the following formulas (4) to (8) (but not limited thereto) , And FIG. 5).

The present inventors 1,1-diphenyl-2-picrylhydrazil according to the concentration (1, 10, 100, 500 or 1000 ㎍ / ㎖) of the centipedes grass extract to confirm the antioxidant activity of the centipedes grass extract ( 1,1-diphenyl-2-picrylhydrazyl (DPPH) inhibitory activity was measured in comparison with corn beard extract, which is well known for its anti-aging activity. As a result, DPPH inhibitory activity of the centipedes grass extract at a concentration of 1 to 1000 μg / ml was about 5, 13, 92, 95 and 95% for methanol extract, 4, 13, 91, 94 and ethanol extract, respectively. 95% and water extracts of about 4, 12, 91, 94, and 94%, respectively, and the corncoal methanol extract showed about 2, 7, 33, 91, and 92% of DPPH inhibitory activity, respectively. Indicated. In particular, the largest difference in DPPH inhibitory activity between the centipedes grass or corn beard extract at the concentration of 100 μg / ml of each extract was shown (see Table 2 and FIG. 6). Therefore, it can be seen that the centipedes grass extract has a significantly superior skin antioxidant activity than the corn beard extract.

In order to examine the antioxidant activity of the centipedes grass extract, fractions thereof or the active fraction isolated from the fractions, the inventors examined the DPPH inhibitory activity of 25 μg / ml centipedes grass extract, fractions thereof and the active fractions isolated from the fractions. Measured. As a result, the active fraction separated from the ethyl acetate fraction of about 53-54%, the centipedes grass extract showed about 89-90% DPPH inhibitory activity (see Table 3 and Figure 7).

Therefore, the centifed grass extract, fractions thereof or the active fractions separated from the fractions can be usefully used as a cosmetic composition effective for preventing skin aging since they exhibit excellent antioxidant activity even in a small amount.

The cosmetic composition may be, for example, an emulsion, a suspension, a microemulsion, a microcapsule, a microgranules or an ionic form (liposomes), a nonionic vesicle obtained by dispersing an oil phase in a solution, a gel, a solid or a dough anhydrous product, or an aqueous phase. It may be provided in the form of a dispersant, cream, skin, lotion, powder, ointment, spray or cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition is a fat substance, organic solvent, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers in addition to the centipedes grass extract of the present invention, fractions or active fractions separated from the fractions of the present invention , Foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or It may contain adjuvants commonly used in the cosmetic field, such as lipophilic actives, lipid vesicles or any other ingredients conventionally used in cosmetics.

In the cosmetic composition containing the centipede grass extract of the present invention, a fraction thereof or an active fraction separated from the fraction, in the cosmetic composition usually contained 1 to 15% by weight, preferably 2 to 10 It can be added in an amount by weight.

In the case of using the centipedes grass extract of the present invention, fractions thereof, or active fractions separated from the fractions as external preparations for skin, additionally, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, Stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments , Hydrophilic or lipophilic actives, lipid vesicles, or any other ingredient commonly used in external preparations for skin, may contain adjuvants commonly used in the field of dermatology. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

     In another aspect, the present invention provides a pharmaceutical composition for preventing skin aging containing Centipedgrass extract, a fraction thereof or an active fraction isolated from the fraction as an active ingredient.

Since the centipedes grass extract of the present invention, fractions thereof or the active fractions isolated from the fractions were found to have DPPH inhibitory activity and showed excellent antioxidant activity (see Table 2, Figures 6 and 7), effective skin aging It can be usefully used as a pharmaceutical composition for prevention.

The pharmaceutical composition of the present invention may further include conventionally used excipients, disintegrants, sweeteners, lubricants, flavoring agents, etc., tablets, capsules, powders, granules, suspensions, emulsions, It can be formulated into syrups, other liquids.

In particular, the pharmaceutical compositions of the present invention may be formulated for oral administration such as, for example, stagnation, troches, lozenges, aqueous or dominant suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or It is formulated with elixirs. Lactose, saccharose, sorbitol, mannitol, starch, amylopectin, binders such as cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, for formulation into formulations such as tablets and capsules, Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the pharmaceutical composition of the present invention may be administered orally or parenterally, and it is preferable to select subcutaneous injection, intravenous injection, intramuscular injection, or intrathoracic injection injection method. In order to formulate into a parenteral dosage form, the centipedes grass extract, fractions thereof or the active fractions isolated from the fractions of the present invention are prepared as a suspension by mixing in water together with a stabilizer or a buffer and preparing a suspension in unit dosage form of ampoules or vials. It may be formulated.

The dosage of the active ingredient according to the present invention is appropriately selected according to the absorption rate, inactivation rate and excretion rate of the active ingredient in the body, the age, sex and condition of the patient, the severity of the disease to be treated, but in the case of oral administration in general Adults can be administered once to several times in an amount of 0.0001 to 500 mg of the extract of the present invention per kg of body weight per day, it is preferable to administer in an amount of 0.001 to 100 mg.

In addition, the present invention provides a skin anti-aging health food containing the centifed glass extract, a fraction thereof or an active fraction isolated from the fraction as an active ingredient.

Centifed glass extract, fractions thereof, or active fractions separated from the fractions of the present invention are excellent in radical scavenging activity and have high antioxidant activity (see Table 2, FIG. 6 and FIG. 7). It can be usefully used.

The health food of the present invention may be added to the centipedes grass extract, fractions thereof, or active fractions separated from the fractions as it is, or used with other foods or food ingredients, and may be appropriately used according to conventional methods.

There is no particular limitation on the kind of food. Examples of foods to which the centifed grass extract, fractions thereof, or active fractions separated from the fractions may be added include meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, other noodles, gums, and ice cream. Dairy products, including various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes, and includes all of the health food in the usual sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the centipedes grass extract of the present invention, fractions thereof, or active fractions separated from the fractions are various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids. Thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. In addition, the centifedgrass extract, fractions thereof, or active fractions separated from the fractions of the present invention may contain pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples, Comparative Examples and Preparation Examples.

However, the following Examples, Experimental Examples, Comparative Examples, and Production Examples specifically illustrate the present invention, and the content of the present invention is not limited to Examples, Experimental Examples, Comparative Examples, and Production Examples.

< Example  1> Sentipedgrass  Preparation of Extract

<1-1> Sentipedgrass  Preparation of Methanol Extracts

Sentipedgrass seeds from Fukukaen Nursery and Blub co. Ltd. was obtained from the Korea Atomic Energy Research Institute Jungeup Institute of Radiation Science Research Institute and collected from the Centipedgrass leaf samples. The collected leaf samples were stored and used at -80 ° C. 5 kg of centipedgrass leaves were added to 20 L of 100% methanol (MeOH) and crushed in a blender, and then the extract obtained by immersion at room temperature for 3 days was concentrated under reduced pressure at 50 ° C. to obtain 210 g of centipedes methanol extract.

<1-2> Sentipedgrass  Preparation of Ethanol Extract

Sentipedgrass seeds from Fukukaen Nursery and Blub co. Ltd. was imported from the Korea Atomic Energy Research Institute Chung-eup Institute of Radiation Science Research Institute and collected from the Centipedgrass leaf. The collected leaf samples were stored and used at -80 ° C. 5 kg of centipedgrass leaves were added to 20 L of 100% ethanol (EtOH) and crushed with a blender, and then the extract obtained by immersion at room temperature for 3 days was concentrated under reduced pressure at 50 ° C. to obtain 203 g of centipedes ethanol extract.

<1-3> Sentipedgrass  Preparation of Water Extract

Sentipedgrass seeds from Fukukaen Nursery and Blub co. Ltd. was imported from the Korea Atomic Energy Research Institute Chung-eup Institute of Radiation Science Research Institute and collected from the Centipedgrass leaf. The collected leaf samples were stored and used at -80 ° C. 5 kg of centipedgrass leaves were added to 20 L of water and crushed with a blender, and the resulting extract was immersed at room temperature for 3 days, and the resulting extract was concentrated under reduced pressure at 50 ° C. to obtain 198 g of centipedes glass water extract.

< Example  2> Sentipedgrass Fraction  And preparation of active fractions

The centifed glass methanol extract obtained in Example 1-1 was fractionated according to polarity using an organic solvent. As shown in FIG. 1, the methanol extract concentrated under reduced pressure was suspended in water, first extracted with hexane (N-Hexane), which is a low polar solvent, and the water layer was partitioned with ethyl acetate (EtOAc). After partitioning into two solutions, a soluble fraction with water was finally obtained. The solvent extract fractions were concentrated under reduced pressure and dried, and an ethyl acetate fraction (21 g) was obtained.

20%, 50% or 70% of ethyl acetate fraction was used as the eluent and Flex-Colum ^TM (3 cm × 25 cm) and Column chromatography was performed using Toyoperarl HW-40 (75 μm), ethyl acetate-20% methanol (9.41 g), ethyl acetate-50% methanol (5.74 g) and ethyl acetate-70% methanol (1 g). Three active fractions were obtained.

< Comparative example  1> Preparation of Corn Beard Extract

Corn beard samples were collected from a farm of Kwangpyeongok varieties from farms in Jecheon, Chungbuk. 5 kg of corn beard was added to 20 L of 80% methanol (MeOH), and then pulverized with a blender. The extract obtained by immersion at room temperature for 3 days was concentrated under reduced pressure at 50 ° C. to obtain 194 g of corn beard methanol extract.

< Experimental Example  1> Sentipedgrass  Extract or its From fractions  Useful secondary present in isolated active fraction Metabolite  analysis

Centipedgrass methanol extract obtained in Example <1-1> using Agilent high performance liquid chromatography (Agilent Technological, Palo Alto, Calif., USA), <Example 2 The useful secondary metabolites of the ethyl acetate-20% methanol, ethyl acetate-50% methanol and ethyl acetate-70% methanol active fractions obtained from > The column used Beckman Coulter ULTRASPHERE (5 μm, 4.6 mm × 25 cm) and the temperature of the column was adjusted to 40 ° C. HPLC was carried out using a mobile phase containing a solution containing 0.1% trifluoroacetic acid (A) and a 50% acetonitrile solution (B) containing 0.1% trifluoroacetic acid as a mobile phase. . According to the mobile phase conditions shown in Table 1 below, the flow rate was maintained at 1 ml / min for 50 minutes.

As a result, the centifedgrass methanol extract contains useful secondary metabolites such as chlorogenic acid, rutin (Quercetin-3-O-rutinoside), and naringinin. Naringin (naringenin-7-O-neohesperidoside), isoorientin, rhamnosylisoorientin, iso-ipoline (apigenin-7-O) -Isotinifol {isorhoifolin (apigenin-7-O-rutinoside)}, isochaptoside (apigenin-6- C- arabinosyl-8-C-glucoside {isoschaftoside (Apigenin-6- C- arabinosyl-8) -C -glucoside) and maysiin (FIG. 2).

Secondary metabolites such as isoorientin, rhamnosylisoorientin, and isoleuropoline (apigenin-7-O-lutinoside) are also useful for centrifuged glass ethyl acetate-20% methanol active fractions. {isorhoifolin (apigenin-7-O-rutinoside)}, isochaptoside (apigenin-6- C- arabinosyl-8-C-glucoside {isoschaftoside (Apigenin-6- C- arabinosyl-8- C -glucoside ) And maysiin (Fig. 3) .The active metabolite isoorientin, rhamnosylisoorientin, useful for centifedgrass ethyl acetate-50% methanol active fractions. ), Isoleuropoline (apigenin-7-O-rutinoside) {isorhoifolin (apigenin-7-O-rutinoside)} and maysiin were shown to exist (FIG. 4). The acetate-70% methanol active fraction contains maysiin, a useful secondary metabolite. It appeared to exist (FIG. 5).

Mobile phase A: 0.1% Trifluoroacetic acid (TFA) B: Acetonitrile: Water (H ₂ O) = 1: 1 (v / v) in 0.1% TFA Extraction mode
(Gradient conditions) Extraction time % Mobile phase B 0 20 18 33 30 40 40 40 42 20 50 20

< Experimental Example  2> DPPH  Determination of antioxidant activity through inhibitory activity

<2-1> Sentipedgrass  Determination of Antioxidant Activity According to the Concentration of Extracts

1,1-diphenyl-2-picrylhydrazyl (DPPH) receives electrons or hydrogen from antioxidants and forms an irreversibly stable molecule, which measures antioxidant activity from electron donating ability. When it encounters a substance with antioxidant activity, free radicals are eliminated and the blue-green color inherent in DPPH is thinned, and the color difference is measured by colorimetric measurement of electron donating ability (Jeon et al. , J. Kor. Soc. Food sic . Nutr . 38 (1): 1-8, 2009). 400 µl of 0.2 mM DPPH was added to 400 µl of the centipedes grass extract obtained in <Example 1> and the corn beard extract obtained in <Comparative Example 1>, and the final concentration of the extract was 1, 10, 100, The absorbance was measured at 517 nm by reacting at 500 or 1000 µg / ml at room temperature for 30 minutes. Ascorbic acid of 1 μg / ml was used as the positive control, and the negative control was measured using the solvent of the extract. DPPH inhibitory activity was expressed as the absorbance decrease rate of the extract addition group and no addition group, and was calculated according to the following [Equation 1].

% Inhibition = (1-absorbance of the sample addition group / absorbance of the no addition group) × 100

As a result, the centifedgrass methanol extract showed DPPH inhibitory activity of about 5, 13, 92, 95 and 95% at concentrations of 1, 10, 100, 500 and 1000 μg / ml, respectively, At concentrations of 1, 10, 100, 500 and 1000 μg / ml, the DPPH inhibitory activity was about 2, 7, 33, 91 and 92%, respectively. At 100 μg / ml, the DPPH inhibitory activity of the centipedes and corn beard methanol extracts was about 92% and 33%, respectively, and the largest difference in DPPH inhibitory activity of the two extracts was confirmed (see Table 2 and FIG. 6). ). Therefore, the centipedes grass extract showed significantly higher anti-aging activity than corn beard extract.

sample Final concentration (㎍ / ㎖) DPPH Inhibition Rate (%) sen
tea
Fe
De
That
la
S






Methanol extract


One 5 10 13 100 92 500 95 1000 95 Ethanol extract


One 4 10 13 100 91 500 94 1000 95 Water extract


One 4 10 12 100 91 500 94 1000 94 jade
Number
Number
Number
salt Methanol extract One 2 10 7 100 33 500 91 1000 92 Ascorbic acid One 98

<2-2> Sentipedgrass  Extract, its Fraction  Or above From fractions  Determination of Antioxidant Activity from Isolated Active Fractions

According to the method of Experimental Example <2-1>, the DPPH inhibitory activity of 25 µg / ml centipedegrass extract, a fraction thereof or an active fraction isolated from the fraction was measured.

As a result, centifedgrass methanol, ethanol and water extract showed DPPH inhibitory activity of about 54.3, 53.1 and 52.9%, respectively, and hexane fraction and water fraction showed DPPH inhibitory activity of about 0.4 and 38.6%, respectively, and ethyl The active fraction isolated from the acetate fraction showed significant DPPH inhibitory activity of about 89-90% (Table 3 and Figure 7).

Sample (25 μg / ml) DPPH Inhibition Rate (%) Methanol extract 54.3 Ethanol extract 53.1 Water extract 52.9 Hexane fraction 0.4 Water fraction 38.6 Ethyl acetate-20% methanol 89.8 Ethyl acetate-50% methanol 89.1 Ethyl Acetate-70% Methanol 89.3

< Manufacturing example  1> Sentipedgrass  Extract, its Fraction  Or above From fractions  For preventing skin aging containing separated active fraction as an active ingredient Cosmetic  Produce

<1-1> preparation of flexible lotion

The flexible purified water containing the centipede glass ethanol extract of Example <1-2> as an active ingredient was prepared as shown in [Table 4].

Raw material Content (parts by weight) Ethanol Extract of Example <1-2> 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<1-2> Preparation of nourishing cream

Nutritional cream containing the centipede glass ethanol extract of Example <1-2> as an active ingredient was prepared as in the composition of the following [Table 5].

Raw material Content (parts by weight) Ethanol Extract of Example <1-2> 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavoring agent Quantity Purified water Balance

< Manufacturing example  2> Sentipedgrass  Extract, its Fraction  Or above From fractions  Preparation of a drug for preventing skin aging containing the separated active fraction as an active ingredient

<2-1> Preparation of Syrup

The syrup containing the centipede glass ethyl acetate-20% methanol active fraction of <Example 2> as an active ingredient was prepared as shown in the following [Table 6].

Ingredient Content (parts by weight) Ethyl Acetate-20% Methanol Fraction of <Example 2> 2 saccharin 0.8 Party 25.4 glycerin 8 Spices 0.04 ethanol 4 Sorbic acid 0.4 Distilled water 60

<2-2> Preparation of Tablet

The tablet containing the centipede glass ethyl acetate-20% methanol active fraction of <Example 2> as an active ingredient was prepared as shown in the following [Table 7].

Ingredient Content (parts by weight) Ethyl Acetate-20% Methanol Fraction of <Example 2> 250 Lactose 175.9 Potato starch 180 Colonde silicic acid 32 10% gelatin solution 5 Potato starch 160 talc 50 Magnesium stearate 5

250 parts by weight of the ethyl acetate-20% methanol active fraction of <Example 2>, 175.9 parts by weight of lactose, 180 parts by weight of potato starch, and 32 parts by weight of colloidal silicic acid were mixed. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. This was dried and the mixture obtained by adding 160 parts by weight of potato starch, 50 parts by weight of talc and 5 parts by weight of magnesium stearate was prepared as a tablet.

< Manufacturing example  3> Sentipedgrass  Extract, its Fraction  Or above From fractions  Preparation of health food for skin aging containing separated active fraction as active ingredient

Foods containing the centipede glass water extract of Example <1-3> as an active ingredient were prepared as follows.

<3-1> Production of flour food

0.5 to 5.0 parts by weight of the extract of Example <1-3> was added to the flour, and bread, cake, cookies, crackers, and noodles were prepared using the mixture.

<3-2> soup  And juicy ( gravies Manufacturing

0.1 to 5.0 parts by weight of the extract of Example <1-3> was added to soups and broths to prepare meat products for health promotion, soups and noodles for noodles.

<3-3> Ground Beef  Produce

10 parts by weight of the extract of Example <1-3> was added to the ground beef to prepare a ground beef for health promotion.

<3-4> Dairy products ( dairy products Manufacturing

5-10 parts by weight of the extract of Example <1-3> was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<3-5> Solar  Produce

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The extract of Example <1-3> was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a spray and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The grains, seeds, and extracts of Example <1-3> prepared above were prepared by combining the following ratios.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Extract (3 parts by weight) of Example <1-3>,

(0.5 part by weight),

Foxglove (0.5 part by weight)

< Manufacturing example  4> Sentipedgrass  Extract, its Fraction  Or above From fractions  Preparation of anti-aging beverage containing separated active fraction as an active ingredient

<4-1> Health drink  Produce

Homogeneous components such as liquid fructose (0.5%), oligosaccharides (2%), sugar (2%), salt (0.5%), water (75%) and 5 g of the extract of Example 1-3 of the present invention are homogeneous. After sterilization and instant sterilization, it was prepared by packaging in small packaging containers such as glass bottles and plastic bottles.

<4-2> Production of vegetable juice

5 g of the extract of Example <1-3> was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice.

<4-3> Preparation of fruit juice

1 g of the extract of Example <1-3> was added to 1,000 ml of apple or grape juice to prepare a fruit juice.

As described above, the centifed glass extract of the present invention, the fractions thereof or the active fractions separated from the fractions have excellent antioxidant activity and have an excellent effect on anti-aging, thus developing cosmetics, external skin preparations, drugs or health foods for anti-aging and It can be usefully used to produce products.

1 is a diagram illustrating a process for preparing an extract of centipedegrass, fractions thereof, and active fractions separated from the fractions.

2 is a diagram showing high performance liquid chromatography identifying secondary metabolites present in centipedegrass methanol extract.

Figure 3 is a diagram showing high performance liquid chromatography identifying secondary metabolites present in centifed glass ethyl acetate-20% methanol active fractions.

FIG. 4 is a diagram showing high performance liquid chromatography identifying secondary metabolites present in centifed glass ethyl acetate-50% methanol active fraction.

FIG. 5 is a diagram showing high performance liquid chromatography identifying secondary metabolites present in centifed glass ethyl acetate-70% methanol active fraction.

Figure 6 is a graph showing the antioxidant activity (DPPH inhibitory activity) according to the concentration of centipede glass methanol extract or corn beard methanol extract.

Figure 7 is a graph showing the antioxidant activity (DPPH inhibitory activity) of the centipedes grass extract, the active fractions (ethyl acetate 20% methanol, -50% methanol and -70% methanol), water or hexane fractions isolated from the fractions thereof to be.

Claims (8)

delete delete delete Ethyl acetate fraction obtained by dissolving the extract extracted from Certigrass ( Eremochloa ophiuroides ) with water, a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof in water and then systematically fractionating it in the order of n-hexane and ethyl acetate. Cosmetic composition for preventing skin aging containing. delete The ethyl acetate fraction obtained by dissolving the extract of centifedgrass with water, lower alcohols of C ₁ to C ₄ or a mixed solvent thereof in water and then systematically fractionating it in the order of n-hexane and ethyl acetate was further adsorbed on column chromatography. A skin cosmetic composition for preventing skin aging containing 20%, 50% or 70% methanol as an eluting solvent. delete delete

Images