KR102228257B1 - Composition for anti-inflammation or anti-acne comprising extract of Immature Citrus Fruit, Curcuma longa and citrus blossom honey - Google Patents

Abstract

The present invention relates to an anti-inflammatory or anti-acne cosmetic composition comprising unripe Citrus unshiu, Curcuma longa and Citrus reticulata flower nectar extracts. The anti-inflammatory or anti-acne cosmetic composition according to the present invention contains unripe Citrus unshiu, Curcuma longa and Citrus reticulata flower nectar, and thus suppresses NO production and the expression of TNF-α, which is an inflammatory mediator, compared to a single extract, thereby significantly improving an inflammation alleviation effect and an antibacterial effect against Propionibacterium acnes.

Description

Composition for anti-inflammation or anti-acne comprising extract of Immature Citrus Fruit, Curcuma longa and citrus blossom honey}

The present invention relates to an anti-inflammatory or anti-acne cosmetic composition comprising a green tangerine, turmeric and citrus flower honey extract.

Inflammation is the body's immune response in response to wounds or diseases, and oxidative stress such as ultraviolet rays, free radicals, etc. activates inflammatory factors, causing various diseases and aging of the skin. Vasoactive polypeptides such as Kinin, Plasmin, or Complement act as vasodilation, contraction, and chemotaxis, and other lymphokines such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for the inflammatory response. Arachidonic acid is metabolized to prostaglandin or Lukotriene, which are inflammatory mediators, through two pathways of Cyclooxygenase or Lipooxygenase, mediating various inflammatory reactions.

On the other hand, in order to eliminate inflammation, it is called an anti-inflammatory agent that acts to remove inflammatory sources, reduce biological reactions, and symptoms. Substances used for anti-inflammatory purposes to date include flufenamic acid, ibuprofen, Benzydamine, or Indomethacin as non-steroids, and Prednisolone as a steroidal system. Or dexamethasone. In addition, allantoin, azene, hydrocortisone, etc. are known to have anti-inflammatory effects, but these substances are limited in use due to safety issues on the skin, or they are insignificant, so there is a problem that the effect of substantially reducing inflammation cannot be expected. have.

Acne (Acne vulgaris) is a chronic inflammatory disease that occurs in the hair follicles and sebaceous glands. The causes of acne can be largely due to increased sebum secretion, abnormal hyperkeratinization of the follicular epithelium, and proliferation of the anaerobic skin flora, Propionibacterium acne. The cause of acne is very complicated because it is affected by physical or mental conditions such as poor physical condition.

Conventionally, acne bacteria are suppressed using antibiotics such as erythromycin and benzoyl peroxide, or acne bacteria are removed using triclosan, salicylic acid, etc. for the treatment of acne. It proliferates and cannot control excessive sebum secretion, which is one of the main causes of acne, so there is a limitation as a fundamental acne treatment method.

Green tangerine is a field tangerine that is shipped until the date set by the governor of Jeju Special Self-Governing Province (August 15th to September 15th, 2018) in compliance with the safe use of pesticides for the purpose of using the functional ingredients of citrus (Citurus unshiu S.). This is a term referring to'. Jeju Special Self-Governing Province Ordinance on Citrus Production and Distribution (Article 2). In other words, it refers to the green tangerine unripe fruit and was called'blue tangerine' until 2015, but it is now called'green tangerine' because it was confused with'blue tangerine' among the traditional citrus varieties of Jeju. Recently, various experiments conducted by institutions have revealed the efficacy of green tangerine to help suppress inflammation and increase skin moisturizing power. Functional ingredients of green tangerine include polyphenols and flavonoids. The total polyphenol content of green tangerine is 19.59g/10g in peel and 4.01g/10g in pulp. Times higher. The content of flavonoids narirutin and hesperidin is 348pm and 326pm in green tangerines, higher than 9pm and 158pm in ripe fruit.In particular, the content of polymethoxy flavonoids nobiletin and anthraxin is 28pm and 9pm in green tangerines. It is known to be four times higher than the 7pm and 1.5pm of the ripe fruit.

Turmeric ( Curcuma longa L. ) is a rhizome of the Ginger family Zingiberaceae. It has anti-inflammatory, anti-allergic, antimicrobial, central suppression, gastric juice secretion, antispasmodic. Actions and the like have been reported. Traditionally, in oriental medicine, turmeric has been used as one of the medicines that help blood circulation, and it has the effect of not only acting on blood, but also making energy well, so it can cure stunned symptoms in the liver and phlegm, enter the blood meal to lower the heat of blood meal, and release blood stagnation. Because it plays a role, it is known as an energy in the blood. Curcuminoid derivatives and essential oils have been reported as the main components of turmeric, and curcuminoid derivatives include curcumin, a diarylheptane and related derivatives, the C9 chain of curcumin. Derivatives (curcumins I-III) and the like have been reported, and as essential oils, it has been reported that there are sesquiterpene-related derivatives such as Turmerone, curlone, and curcumene.

Honey is a natural food produced by honeybees (Apis mellifera L.) by transferring and accumulating substances collected from plant flowers into a honeycomb. It is caused by environmental conditions due to geographical differences from pollen. Honey has been reported to exhibit physiological activities such as antioxidant, antibacterial, whitening, burn treatment, and anti-inflammatory effects, and recently, it has been reported that the antioxidant and antibacterial activities of honey fractions produced in Korea vary depending on the species and region (Baek Et al., 2015; Lee and Lee, 2016). Despite these studies being reported, studies on the physiological activity of domestic honey fractions are insignificant. The citrus flower honey of the present invention refers to honey collected from an apiary near a citrus farm in Jeju. Citrus flowers are white flowers that bloom only two weeks a year, and the honey collected at this time is called citrus flower honey. Honey is known to contain phenolic acid, flavonoids, glucose oxidase, catalase, organic acids, and amino acids.

Korean Patent Publication No. 10-2019-0001163

It is an object of the present invention to provide an anti-inflammatory or anti-acne cosmetic composition.

Another object of the present invention is to provide a health functional food composition for preventing or improving inflammation.

Another object of the present invention is to provide a health food composition for preventing or improving inflammation.

To achieve the above object,

The present invention provides a cosmetic composition for anti-inflammatory or anti-acne containing green tangerine, turmeric and citrus flower honey extract.

In addition, the present invention provides a health functional food composition for preventing or improving inflammation, containing green tangerine, turmeric and citrus flower honey extract.

Furthermore, the present invention provides a health food composition for preventing or improving inflammation, containing green tangerine, turmeric and citrus flower honey extract.

The green tangerine, turmeric or citrus flower nectar extract may be used hot water extraction, cold sedimentation, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, compression, and the like.

The green tangerine, turmeric or citrus flower honey extract may be extracted by alone or by mixing an extraction solvent such as water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, butylene glycol.

The composition for anti-inflammatory or anti-acne according to the present invention contains green tangerine, turmeric, and citrus flower honey to reduce inflammation by inhibiting NO production and expression of TNF-α, an inflammatory mediator, compared to a single extract, and antibacterial effect against P. acnes. There is an effect that the effect is remarkably improved.

1 is a schematic diagram of a method for preparing a green tangerine extract.
Figure 2 is a schematic diagram showing a method for producing a turmeric extract.
Figure 3 is a schematic diagram showing a simple method of manufacturing a citrus flower honey extract.

The present inventors completed the present invention by discovering that the composition containing the green tangerine, turmeric and citrus flower honey extract exhibits anti-inflammatory and anti-acne effects.

In particular, in the present invention, when a composition in which green tangerine, turmeric and citrus flower honey extracts are mixed in a specific composition ratio is used, the anti-inflammatory and anti-acne effects are significantly improved compared to a composition containing some of these ingredients or a composition containing other composition ratios. Was proved experimentally (see Experimental Examples 1 to 3).

The green tangerine of the present invention refers to the term "noji tangerine determined by the governor of Jeju Special Self-Governing Province in compliance with the safe use of pesticides for the purpose of using the functional ingredients of citrus (Citurus unshiu S.)."

Turmeric of the present invention ( Curcuma longa Radix ) is a plant of the ginger family and generally refers to the tuber root of Curcuma longa. It grows or grows in Southeast Asia, including southern China, India, and Okinawa, and is also cultivated in the central and southern regions of Korea.

The citrus flower honey of the present invention refers to honey collected from an apiary near a citrus farm in Jeju. Citrus flowers are white flowers that bloom only two weeks a year, and the honey collected at this time is called citrus flower honey.

Hereinafter, the present invention will be described in more detail.

Anti-inflammatory or anti-acne cosmetic composition containing green tangerine, turmeric and citrus flower honey extracts

The present invention provides a cosmetic composition containing green tangerine, turmeric and citrus flower honey extract.

Based on 100 parts by weight of the green tangerine extract, 100 parts by weight of turmeric extract and 10-100 parts by weight of citrus flower honey extract may be included.

Preferably, based on 100 parts by weight of the green tangerine extract, 100 parts by weight of turmeric extract and 20-40 parts by weight of citrus flower honey extract may be included.

In addition, the term'extract' in the present invention means that the extract is separated and obtained from the raw material of the active ingredient using a method for extracting and separating known in the art, and the extract defined in the present invention is the above using an appropriate solvent. The active ingredient raw material prepared by the method of may be extracted as it is or pulverized. At this time, any suitable solvent that can be used to obtain the extract may be used as long as it is an acceptable solvent in the art, and water or an organic solvent may be used. For example, water, an alcohol having 1 to 5 carbon atoms, an extraction solvent such as hexane, chloroform, or butylene glycol may be used alone or in combination, but is not limited thereto.

As the extraction method, methods such as hot water extraction, cold precipitation extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, compression, and the like can be used. In addition, the desired extract may be further subjected to a conventional fractionation process, or may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

For example, the extract contained in the composition of the present invention may be used as it is while the primary extract extracted by the solvent extraction method described above is centrifuged, filtered, concentrated, freeze-dried, and stored in a refrigerator. It can be prepared in a powder state by additional processes such as distillation and freeze drying or spray drying. Further, the primary extract was further purified by using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get

Therefore, in the present invention, the extract is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, their dilutions, concentrates or dried products.

In addition, the cosmetic composition of the cosmetic composition according to the present invention is a serum, lotion, essence, paste, mask pack, patch, gel, cream, lotion, nutrition lotion, nutrition cream, moisture cream, massage cream, powder, soap, cleanser, It can be prepared and used in formulations such as oil, foundation, makeup base, wax, and spray.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and/or bis. It may be provided in the form of a warm vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray, or conceal stick. In addition, it may be prepared in the form of a foam (foam) or in the form of an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of an adjuvant that can be applied topically or systemically used in the field of dermatology by containing a dermatologically acceptable medium or base. It can be manufactured according to the method.

In addition, the cosmetic composition is a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent in addition to the composition containing the green tangerine, turmeric and citrus flower honey extract of the present invention ( foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives , Lipid vesicles or any other ingredients commonly used in cosmetics.

In the cosmetic composition containing the green tangerine, turmeric and citrus flower honey extract of the present invention, the green tangerine, turmeric and citrus flower honey extract of the present invention is 0.1 to 50% by weight, preferably 1 to 10% by weight, in the cosmetic composition normally contained It may be added in an amount of, more preferably, it may be added in an amount of 0.1 to 5% by weight.

When the cosmetic composition containing the green tangerine, turmeric and citrus flower honey extract of the present invention is used as an external preparation for the skin, additionally fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, Foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or hydrophilic It may contain adjuvants commonly used in the field of dermatology such as oily active agents, lipid vesicles or any other ingredients commonly used in topical preparations for skin. In addition, the ingredients may be introduced in an amount generally used in the field of dermatology.

Health functional food and health food composition for preventing or improving inflammation containing green tangerine, turmeric and citrus flower honey extract

The present invention provides a health functional food composition for preventing or improving inflammation, containing green tangerine, turmeric and citrus flower honey extract.

In addition, the present invention provides a health food composition for preventing or improving inflammation, containing green tangerine, turmeric and citrus flower honey extract.

In addition, the green tangerine, turmeric and citrus flower honey extracts are preferably contained in an amount of 0.1 to 99% by weight based on the total weight of the health functional food or health food composition.

There are no special restrictions on the type of food. Examples of foods to which the green tangerine, turmeric and citrus flower honey extract of the present invention can be added include drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice creams. Dairy products including, various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and dairy products, etc., including all health foods and health functional foods in the usual sense.

The term "health functional food" refers to a food manufactured and processed using raw materials or ingredients having useful functions for the human body according to the Health Functional Food Act No. 6727, and the term "functional" refers to the structure of the human body. And it means ingestion for the purpose of obtaining useful effects for health use such as controlling nutrients for function or physiological action.

The food composition of the present invention may contain conventional food additives, and whether or not it is suitable as a "food additive" is determined according to the general rules and general test methods of food additives approved by the Food and Drug Administration, unless otherwise specified. It is judged according to the standards and standards for the item.

Items listed in the "Food Additives Code" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as reduced pigments, licorice extract, crystalline cellulose, high color pigments, and guar gum, And mixed preparations such as sodium L-glutamate preparation, an alkali additive for noodles, preservative preparations, and tar coloring preparations.

Among the capsule-type health functional foods, hard capsules can be prepared by filling a mixture of herbal extracts with additives such as excipients or granular or skinned granules in ordinary hard capsules, and soft capsules can be prepared by filling herbal extracts with excipients, etc. It can be prepared by filling a mixture with the additive of gelatin in a capsule base. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.

Ring-shaped health functional foods can be prepared by molding a mixture of excipients, binders, and disintegrants into herbal extracts in an appropriate way. It can also be pleasing.

The health functional food in the form of granules may be prepared in a granular form by a suitable method of a mixture of an excipient, a binder, a disintegrant, etc. to the herbal extract, and may contain flavoring agents, flavoring agents, etc., if necessary. The definitions of terms for the excipients, binders, disintegrants, lubricants, flavoring agents, flavoring agents, etc. of the present invention are described in documents known in the art, and include those having the same or similar functions (the Korean Pharmacopoeia commentary, Seongsa, Korea Pharmaceutical University Council, 5th revised edition, p33-48, 1989).

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. However, the following examples are only for embodiing the contents of the present invention, and the present invention is not limited thereto.

<Production Example 1> Preparation of green tangerine extract

A green tangerine extract was prepared with reference to FIG. 1. Citrus ( Citurus unshiu S. ) In 100 g of lyophilized green tangerine powder of immature fruit, add 900 mL of distilled water as an extraction solvent, and extract the extract at 60℃ for 9-11 hours in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea). Got it. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh). The filtered filtrate was concentrated under reduced pressure using a rotary evaporator (DL10-3000, Greatwall, 20L) to remove the solvent to prepare a green tangerine extract. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

<Production Example 2> Preparation of turmeric extract

A turmeric extract was prepared with reference to FIG. 2. 50 g of dried turmeric slices were immersed in 950 ml of a 40% butylene glycol solution for 3 days at room temperature for extraction to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh). The filtered filtrate was concentrated under reduced pressure using a rotary evaporator (DL10-3000, Greatwall, 20L) to remove the solvent to prepare a turmeric extract. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

<Preparation Example 3> Preparation of citrus flower honey extract

A citrus flower honey extract was prepared with reference to FIG. 3. 100 g of the selected citrus flower honey was immersed in a 30% butylene glycol solution for 7 days at 4° C. and extracted to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh). The filtered filtrate was concentrated under reduced pressure using a rotary evaporator (DL10-3000, Greatwall, 20L) to remove the solvent to prepare a citrus flower honey extract. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

<Examples and Comparative Examples> Preparation of a mixed solution of green tangerine, turmeric and citrus flower honey extract

A mixed solution containing green tangerine, turmeric and citrus flower honey extract was prepared in the composition of Table 1 and Table 2 below.

At this time, each extract was mixed in the composition of Table 1 below, and the extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

Weight ratio Green tangerine extract Turmeric extract Citrus Flower Honey Extract Example 1 One One One Comparative Example 1 One - - Comparative Example 2 - One - Comparative Example 3 - - One Comparative Example 4 One One -

In order to find out the optimal mixing ratio of the mixed solution containing green tangerine, turmeric and citrus flower honey extract, it was prepared in the composition of Table 2 below.

At this time, each extract was mixed in the composition of Table 2 below, and the extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

Weight ratio Green tangerine extract Turmeric extract Citrus Flower Honey Extract Example 2-1 One One 0.1 Example 2-2 One One 0.2 Example 2-3 One One 0.3 Example 2-4 One One 0.4 Example 2-5 One One 0.5 Example 2-6 One One 0.6 Example 2-7 One One 0.7 Example 2-8 One One 0.8 Example 2-9 One One 0.9 Example 2-10 One One 1.0

<Experimental Example 1> NO production inhibitory effect

In order to investigate the anti-inflammatory effect of green tangerine, turmeric and citrus flower honey extracts, the inhibitory effect of NO production was confirmed. RAW 264.7 cells, which are mouse macrophages, ^{were dispensed into a 60mm plate at a density of 2 X 10 6} cells/dish, and cultured on the plate for 24 hours. In order to induce an inflammatory reaction, a new medium containing LPS (lipopolysaccharide) at a concentration of 1 μg/ml and a test substance was treated and cultured for 24 hours. Anti-inflammatory activity evaluation through NO assay was performed using griess A and B reagents, and 1% sulfanilamide and 0.1% N-(1-naphtyl)ethylenediamine dihydrochloride reagent were mixed in a 1:1 ratio. After treating Example 1, Examples 2-1 to 2-10 and Comparative Examples 1 to 4 in a 96 well plate, the supernatant of the medium cultured for 24 hours at _{37°C and 5% CO 2 was griess (A+B} ) After mixing 50 µl each with a reagent in a 1:1 ratio and storing at room temperature for 10 minutes, the ability to inhibit NO production was measured through ELISA measurement at 540 nm. The results are shown in Table 3 below. The NO generation inhibition rate (%) refers to the value obtained by subtracting the NO generation rate (%) from 100% based on the control group (100%).

division NO production inhibition rate (%) Example 1 48.6 Comparative Example 1 18.3 Comparative Example 2 16.6 Comparative Example 3 23.0 Comparative Example 4 32.9 Example 2-1 48.9 Example 2-2 66.5 Example 2-3 68.1 Example 2-4 66.8 Example 2-5 49.1 Example 2-6 49.4 Example 2-7 50.1 Example 2-8 49.0 Example 2-9 48.9 Example 2-10 48.6

As shown in Table 3, the samples of Examples 2-1 to 2-10 were found to significantly improve the NO generation inhibition rate compared to Comparative Examples 1 to 4. Specifically, when the Colby's equation predicted value was calculated based on the NO generation inhibition rate of Comparative Example 3 and Comparative Example 4, 48.33% was obtained, and the measured values of Examples 2-1 to 2-10 were all Colby equation predicted values. It was confirmed that a synergistic effect appeared by showing a result that was higher than that. In particular, the samples of Examples 2-2 to 2-4 showed a remarkably high inhibition rate of NO generation.

<Experimental Example 2> Inhibitory effect of inflammatory cytokine TNF-α expression

In order to investigate the anti-inflammatory effect of the extracts of green tangerine, turmeric and citrus flower honey, the inhibitory effect of the expression of the inflammatory cytokine TNF-α was confirmed. RAW 264.7 cells, which are mouse macrophages, ^{were adjusted to 5×10 5} cells/well using DMEM medium added with 10% FBS, and then inoculated into 12 well plates and cultured for 18 hours. After incubation, the starvation was maintained for 12 hours, and then 10 ng/mL of LPS, Examples 1, 2-1 to 2-10, and Comparative Examples 1 to 4 were treated, and cultured for 24 hours. RNA was extracted from the cultured cells and the expression rate of TNF-α was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The TNF-α expression rate was calculated by Equation 1 below, and the TNF-α expression inhibition rate refers to a value minus the TNF-α expression rate from 100%. The results are shown in Table 4 below.

[Equation 1]

TNF-α expression rate (%) = (sample TNF-α expression amount / control group TNF-α expression amount) × 100

division TNF-α expression inhibition rate (%) Control 0.0 Example 1 43.0 Comparative Example 1 17.1 Comparative Example 2 8.6 Comparative Example 3 24.9 Comparative Example 4 24.0 Example 2-1 43.4 Example 2-2 55.0 Example 2-3 56.1 Example 2-4 55.7 Example 2-5 43.6 Example 2-6 44.2 Example 2-7 45.6 Example 2-8 44.1 Example 2-9 43.6 Example 2-10 43.0

As shown in Table 4, the samples of Examples 2-1 to 2-10 were found to significantly improve the inhibition rate of TNF-α expression compared to Comparative Examples 1 to 4. Specifically, when calculating the predicted value of the Colby's equation based on the TNF-α expression inhibition rate of Comparative Example 3 and Comparative Example 4, 42.92% was obtained. It was confirmed that a synergistic effect appeared by showing the result exceeding the predicted value. In particular, the samples of Examples 2-2 to 2-4 showed remarkably high inhibition of TNF-α expression.

<Experimental Example 3> P. acnes antibacterial activity measurement

Antibacterial activity against Propionibacterium acnes was measured to determine the anti-acne effect of the extracts of green tangerine, turmeric and citrus flower honey.

In the measurement of the antimicrobial activity of Examples 1 to 2-10 and Comparative Examples 1 to 4, a minimum inhibitory concentration (MIC) was measured using a solid culture dilution method (Agar Serial Dilution Method). As the culture medium, Reinforced clostridial medium (Merck, Germany) was used, and the strain cultured for 72 hours was first ^{diluted to 1.5x10 8} cfu/ml and inoculated into each well, followed by samples of Examples 1 to 2-10 and Comparative Examples. 1 to 4 were treated for each concentration. The minimum inhibitory concentration (MIC) was defined as the minimum inhibitory concentration (MIC) after incubating the well plate inoculated with the bacteria dilution and samples in an anaerobic condition, 37°C incubator for 3 days. Indicated. In this case, the smaller the value of the minimum inhibitory concentration means that the antibacterial effect is higher.

division Minimum inhibitory concentration (MIC, μg/mL) Example 1 1.51 Comparative Example 1 4.62 Comparative Example 2 5.25 Comparative Example 3 8.51 Comparative Example 4 3.37 Example 2-1 1.46 Example 2-2 0.75 Example 2-3 0.73 Example 2-4 0.77 Example 2-5 1.43 Example 2-6 1.55 Example 2-7 1.57 Example 2-8 1.58 Example 2-9 1.57 Example 2-10 1.59

As shown in Table 5, it was confirmed that more than a single composition of green tangerine extract, turmeric extract, and honey extract showed strong antibacterial power against acne strains when treated with complex extracts. All of the samples of Examples 2-1 to 2-10 showed remarkably improved antibacterial power against P. acnes compared to Comparative Examples 1 to 4, and in particular, the samples of Examples 2-2 to 2-4 were P. acnes. The antibacterial power for was remarkably high.

Cosmetic formulation example

The green tangerine, turmeric and citrus flower honey extract according to the present invention can be prepared in various types of cosmetics depending on the purpose. The following exemplifies a method for preparing some cosmetics containing green tangerine, turmeric and citrus flower honey extract according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Formulation Example 1> Preparation of flexible lotion

10.0 parts by weight of green tangerine, turmeric and citrus flower honey extract

1,3-butylene glycol 1.00 parts by weight

0.05 parts by weight of disodium EDTA

0.10 parts by weight of allantoin

0.05 parts by weight of dipotassium glycyrrhizate

0.01 parts by weight of citric acid

Sodium citrate 0.02 parts by weight

Glyceres-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated Castor Oil 1.00 parts by weight

Ethanol 30.0 parts by weight

Trace amounts of preservatives

Trace amount of colorant

A trace amount of flavoring agent

Remaining amount of purified water

<Formulation Example 2> Preparation of nourishing cream

10.0 parts by weight of green tangerine, turmeric and citrus flower honey extract

1,3-butylene glycol 7.00 parts by weight

1.00 parts by weight of glycerin

0.10 parts by weight of D-panthenol

3.20 parts by weight of plant extract

Magnesium aluminum silicate 0.30 parts by weight

1.20 parts by weight of PEG-40 stearate

2.00 parts by weight of stearic acid

1.50 parts by weight of polysorbate 60

2.00 parts by weight of lipophilic glyceryl stearate

1.50 parts by weight of sorbitan sesquioleate

3.00 parts by weight of cetearyl alcohol

4.00 parts by weight of mineral oil

3.80 parts by weight of squalane

2.80 parts by weight of carlylic/capric triglyceride

1.80 parts by weight of vegetable oil

Dimethicone 0.40 parts by weight

Trace amount of diposium glycyrrhizate

Trace amounts of allantoin

Sodium hyaluronate trace amount

Appropriate amount of tocopheryl acetate

Appropriate amount of triethanolamine

Appropriate amount of preservative

Suitable amount of flavoring agent

Remaining amount of purified water

Manufacturing example of health food

The green tangerine, turmeric and citrus flower honey extract according to the present invention can be prepared as various types of health food depending on the purpose. The following exemplifies a method for preparing some health foods containing green tangerine, turmeric and citrus flower honey extracts according to the present invention as active ingredients, and the present invention is not limited thereto.

<Health Food Manufacture Example 1> Manufacture of dairy products

0.01-1 parts by weight of the green tangerine, turmeric and citrus flower honey extract of the present invention were added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health Food Manufacture Example 2> Manufacture of Sunsik

Brown rice, barley, glutinous rice, and adlay were gelatinized and dried by a known method, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder. Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder. The green tangerine, turmeric and citrus flower honey extracts of the present invention were concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. The grains, seeds and green tangerine, turmeric and citrus flower honey extract prepared above were mixed in the following ratio to prepare dry powder.

Cereals (34 parts by weight of brown rice, 19 parts by weight of barley, 20 parts by weight of barley),

Seeds (perilla 7 parts by weight, black soybean 8 parts by weight, black sesame 7 parts by weight),

Green tangerine, turmeric and citrus flower honey extract (2 parts by weight),

Ganoderma lucidum (1.5 parts by weight), and

Rehmannia (1.5 parts by weight).

Example of manufacturing health functional food

The green tangerine, turmeric and citrus flower honey extract according to the present invention can be prepared as various types of health functional foods depending on the purpose. The following exemplifies a method for preparing some health functional foods containing green tangerine, turmeric and citrus flower honey extract according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Production Example 1 of Health Functional Food> Preparation of Health Functional Food

100 mg of green tangerine, turmeric and citrus flower honey extract

The right amount of vitamin mixture

Vitamin A acetate 70 μg

1.0 mg of vitamin E

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

Vitamin B12 0.2 μg

10 mg of vitamin C

Biotin 10 μg

1.7 mg of nicotinic acid amide

50 μg folic acid

0.5 mg of calcium pantothenate

Suitable amount of inorganic mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dicalcium phosphate 55 mg

90 mg of potassium citrate

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is a mixture of ingredients suitable for a health functional food in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, granules are prepared, and can be used for preparing a health functional food composition according to a conventional method.

<Production Example 2 of Health Functional Food> Preparation of Health Functional Beverage

100 mg of green tangerine, turmeric and citrus flower honey extract

100 mg citric acid

100 mg oligosaccharide

Plum Concentrate 2 mg

100 mg taurine

Total 500 mL by adding purified water

After mixing the above ingredients according to a conventional health drink manufacturing method, the resulting solution is stirred and heated at 85°C for about 1 hour, and the resulting solution is filtered and obtained in one sterilized container, sealed and sterilized, and then stored in a refrigerator. It is used in the manufacture of health drink composition. The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the blending ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

As described above, the specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention can add, change, delete other elements within the scope of the same idea, and other regressive inventions. However, it will be possible to easily propose other embodiments included within the scope of the spirit of the present invention. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects. The scope of the present invention is indicated by the scope of the claims to be described later rather than the detailed description described above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. Should be interpreted as.

Claims (8)

Based on 100 parts by weight of green tangerine extract, 100 parts by weight of turmeric extract and 20-40 parts by weight of citrus flower honey extract, anti-inflammatory or anti-acne cosmetic composition.
delete delete The method of claim 1,
The cosmetic composition includes serum, lotion, essence, paste, mask pack, patch, gel, cream, lotion, nutrition lotion, nutrition cream, moisture cream, massage cream, powder, soap, cleanser, oil, foundation, makeup base, wax, and Cosmetic composition for anti-inflammatory or anti-acne, characterized in that at least one formulation selected from the group consisting of spray.
The method of claim 1,
The green tangerine, turmeric or citrus flower honey extract is extracted using a hot water extraction method, a cold sedimentation method, a reflux cooling extraction method, a solvent extraction method, a steam distillation method, an ultrasonic extraction method, an elution method or a compression method, and an anti-inflammatory or anti-acne cosmetic composition.
The method of claim 1,
The green tangerine, turmeric or citrus flower honey extract is extracted with water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, butylene glycol, or a mixed solvent thereof, anti-inflammatory or anti-acne cosmetic composition.
Based on 100 parts by weight of green tangerine extract, containing 100 parts by weight of turmeric extract and 20-40 parts by weight of citrus flower honey extract, a health functional food composition for preventing or improving inflammation.
Based on 100 parts by weight of green tangerine extract, containing 100 parts by weight of turmeric extract and 20-40 parts by weight of citrus flower honey extract, a health food composition for preventing or improving inflammation.

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