KR102481095B1 - Inner beauty composition for anti-wrinkle containing red sea cucumber and fish collagen - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating skin wrinkles comprising red sea cucumber, fish collagen, green tangerine, Beta vulgaris, and Citrus reticulata, and has a remarkable improvement effect on skin wrinkle alleviation, and can be useful as a health food or cosmetic for inner beauty. An object of the present invention is to provide a health food composition for preventing or alleviating skin wrinkles. Another object of the present invention is to provide a jelly composition.

Description

Inner beauty composition for anti-wrinkle containing red sea cucumber and fish collagen}

The present invention relates to an inner beauty composition for preventing or improving skin wrinkles containing red sea cucumber, fish collagen, green tangerine, beet, and citrus flower honey, and the composition can be used as a cosmetic or a health food for inner beauty.

The skin plays an important role in maintaining homeostasis in the body by protecting the body from the external environment. However, due to various external stimuli and unnecessary metabolites generated from internal metabolic processes, skin function is deteriorated and aging is accelerated. Various causes of wrinkle formation have been reported. First of all, when the skin is exposed to excessive ultraviolet rays, the decomposition of collagen, which is a skin component, is promoted, so that the skin reads elasticity and wrinkles may be generated. Collagen in the skin is a structural protein produced by skin fibroblasts, and it is known that the decrease in production and changes in the corresponding structure due to aging are related to the formation of wrinkles in the skin. Therefore, homeostasis in the body related to the production of collagen in the skin and the growth of keratin stem cells is a closely related factor that affects the health and beauty of the skin, and research for the function and discovery of effective materials is still being actively conducted. The effectiveness of the treatment material requires a lot of effort due to the technical difficulties of passing through the epidermis and dermis.

The human body is mostly composed of water, which plays an important role in maintaining life. In normal skin, 70% of moisture is contained in the body and 20% in the stratum corneum, which supports the elasticity and flexibility of the skin. It is known that when the moisture content of the stratum corneum is less than 10%, the skin becomes dry and causes skin trouble. The skin's water balance is regulated by the epidermis. The stratum corneum of the epidermis is a pile of dead cells, but it plays a very important role in maintaining moisture in the skin. The amount of water in the stratum corneum created by regular keratinization always maintains 10-20%. This is because of the sebum film, which is a mixture of sweat and sebum, protecting the skin from the outside and preventing moisture from evaporating from the stratum corneum. Natural Moisturizing Factor (NMF) and dermal lipids hold and retain moisture. The sebaceous membrane, NMF and dermal lipids work together to retain moisture in the skin. NMF is produced in the process of keratinization and plays an important role in moisturizing the skin as a hydrophilic hygroscopic substance. It is made of amino acids and holds moisture in the horny layer. Also, among NMFs, 2-pyrrolidine-5-carboxylic acid (PCA) is particularly important. It forms a layer with a matrix filled between each cell and maintains moisture inside (Journal of Skin Barrier Society, 4, 71-80, 2002).

Recently, the beauty market is diversifying, and with the launch of multi-functional cosmetics and the trend of inner beauty pursuing inner beauty, the era of so-called edible cosmetics and inner beauty brands heralding the future growth beauty market is opening.

Inner beauty refers to inner beauty, and it does not temporarily improve the surface of the skin through artificial methods such as applying cosmetics, but takes care of the health inside the skin through 'eating habits' and 'life habits' to improve the skin itself. As people's desire to be beautiful in the sense of being good grows, interest in inner beauty, which is called edible cosmetics, is increasing.

Ingestible cosmetics have problems such as insufficient functionality such as wrinkle improvement and whitening effects compared to cosmetics applied directly to the human skin, and active ingredients that relieve or improve skin diseases are composed of only a single substance, such as skin that occurs through various routes. There is also a problem that efficient skin care cannot be performed because the causative factor of the related disease cannot be targeted.

Therefore, the inventors of the present invention intensively studied a health functional food composition for inner beauty that is comparable or superior in functionality to cosmetics applied directly to the skin, and in order to discover effective materials for improving skin wrinkles, red sea cucumber concentrate, fish The present invention was completed by finding that a composition containing collagen, green tangerine juice, beet juice, and citrus flower honey had a remarkable synergistic effect on skin wrinkle improvement.

Korea Patent Registration 10-2016027

An object of the present invention is to provide a health food composition for preventing or improving skin wrinkles.

Another object of the present invention is to provide a jelly composition.

Another object of the present invention is to provide a cosmetic composition for preventing or improving skin wrinkles.

Another object of the present invention is to provide a health functional food composition for preventing or improving skin wrinkles.

In order to achieve the above purpose,

The present invention provides a health food composition for preventing or improving skin wrinkles comprising red sea cucumber concentrate, fish collagen, green tangerine juice, beet juice and citrus flower honey.

Preferably, based on 5 parts by weight of red sea cucumber concentrate, 4-6 parts by weight of fish collagen, 19-21 parts by weight of green tangerine juice, 9-11 parts by weight of beet juice, and 0.9-1.1 parts by weight of citrus honey.

The manufacturing method of the red sea cucumber concentrate was disclosed in Preparation Example 1.

An example of the health food according to the present invention may be an inner beauty food, preferably a jelly-type inner beauty food.

In addition, the present invention is based on 59 parts by weight of jelly base, 4-6 parts by weight of red sea cucumber concentrate, 4-6 parts by weight of fish collagen, 19-21 parts by weight of green tangerine juice, 9-11 parts by weight of beet juice, and 0.9 parts by weight of citrus flower honey It provides a jelly composition characterized in that it comprises -1.1 parts by weight.

The jelly base is any commercially available food gel containing vitamin C, enzyme-modified stevia, elastin, purified water, fructooligosaccharide, chicory dietary fiber, locust bean gum, xanthan gum, glucose, agar, tamarind gum, etc. Can be used without restrictions.

In addition, the present invention provides a cosmetic composition for preventing or improving skin wrinkles comprising red sea cucumber concentrate, fish collagen, green tangerine juice, beet juice, and citrus flower honey.

Furthermore, the present invention provides a health functional food composition for preventing or improving skin wrinkles comprising red sea cucumber concentrate, fish collagen, green tangerine juice, beet juice, and citrus flower honey.

The composition comprising red sea cucumber concentrate, fish collagen, green tangerine juice, beet juice, and citrus flower honey according to the present invention has a significant improvement effect on skin wrinkles, and can be useful as a health food or cosmetic for inner beauty.

Hereinafter, the present invention will be described in detail.

Health food/health functional food composition

There is no particular limitation on the types of health food and health functional food. Examples of health functional foods to which the active substances of the present invention can be added include jelly, drinks, tablets, capsules, powders, pills, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, confectionery, pizza, ramen, Other dairy products including noodles, chewing gum, and ice cream, various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and milk-processed products, etc., include both health food and health functional food in a conventional sense.

Health food and health functional food compositions containing active substances according to the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active substance may be appropriately determined according to its purpose of use (for prevention or improvement). In general, the amount of the composition in health food and health functional food may be added in an amount of 0.1 to 90 parts by weight based on the total weight of food. However, in the case of long-term intake for the purpose of health maintenance or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

The health food and health functional food composition of the present invention is not particularly limited in other ingredients except for containing the active substance of the present invention as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates are added as additional ingredients like conventional beverages. may contain Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (eg rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can advantageously be used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 of the health food and health functional food composition of the present invention.

In addition to the above, the health food and health functional food composition containing the active substances of the present invention are various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the health food and health functional food composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages.

These components may be used independently or in combination. The ratio of these additives is not so critical, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health food and health functional food composition containing the active substance of the present invention.

cosmetic composition

The cosmetic composition is, for example, a solution, gel, solid or kneaded anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule or ionic (liposome), nonionic vesicle dispersant It may be provided in the form of a cream, toner, lotion, powder, ointment, spray or conceal stick. In addition, it can be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition includes a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or commonly used in cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are

In the cosmetic composition containing the active material of the present invention, the active material of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, in the cosmetic composition that is usually contained.

When the active substance of the present invention is used as an external skin preparation, additionally, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, and a surfactant , water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles, or external preparations for skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as In addition, the components may be introduced in an amount generally used in the field of skin science.

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

<Preparation Example 1> Preparation of red sea cucumber concentrate

Based on 1 part by weight of red sea cucumber raw material,

Mixing 20 parts by weight of purified water,

After mixing 0.01 parts by weight of hydrolase (Thermolysin: EC 3.4.24.27. Microorganism-derived metalloproteinase produced by Bacillus thermoproteolyticus) and reacting at 55 ± 2 ° C for 6 hours,

concentrated to 5 brix or more;

After treatment at 95 ° C. for 1 hour to inactivate the hydrolase and sterilize,

The red sea cucumber concentrate was prepared by filtering with a 100 mesh filter.

<Preparation Example 2> Preparation of low-molecular-weight fish collagen

Low molecular weight fish collagen (manufacturer: GELTECH, model name: low molecular weight fish collagen) having an average molecular weight of 1000 Mw or less was prepared.

<Preparation Example 3> Preparation of green tangerine juice

Green tangerines from Jeju were subjected to a juice extraction process to prepare a juice solution.

<Preparation Example 4> Preparation of beet juice

Juice was prepared through a juice extraction process from Jeju beets.

<Preparation Example 5> Manufacture of citrus flower honey

Jeju tangerine flower honey was prepared as it was.

<Preparation Example 6> Preparation of jelly base

A jelly base was prepared by mixing vitamin C, enzyme-treated stevia, elastin, purified water, fructooligosaccharide, chicory dietary fiber, locust bean gum, xanthan gum, glucose, agar, and tamarind gum.

<Experimental Example 1> Wrinkle improvement activity evaluation through measurement of procollagen expression level

Collagen is synthesized into procollagen within the cell, then secreted out of the cell and polymerized into collagen fibers. Collagen is a structural protein constituting the skin, and wrinkles of the skin are improved when the content of collagen in the skin is high and when the activity of collagenase is low.

Specifically, human procollagen type I C-terminal peptide (PIP) secreted extracellularly using human dermal fibroblasts was confirmed with the Procollagen Type I C-Peptide (PIP) EIA kit to produce procollagen, The results are shown in Table 1 below. In Table 1 below, the increase rate of procollagen expression compared to the sample untreated group was shown as a percentage.

As shown in Table 1, it can be confirmed that the expression level of procollagen is significantly high in samples obtained by mixing green tangerine juice, beet juice, and citrus flower honey with red sea cucumber concentrate and fish collagen in a specific mixing ratio. On the other hand, Comparative Examples 1 to 6 are samples that do not contain some of the 5 active ingredients, and the expression level of procollagen is found to be at a flat level. It can be seen that there is a significant improvement effect.

<Experimental Example 2> Evaluation of anti-wrinkle activity by measuring elastase activity inhibition rate

The elastase activity inhibitory effect was measured as follows using the method described in the literature (Lee et al., J. Cosmetic Science, 49, 285-297, 1998).

Specifically, the substrate solution (elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard (SIGMA, S4760) at 8.8 mM) and 20 μl of sample (prepared by diluting samples of Examples and Comparative Examples to a concentration of 400 ppm) and mixing, enzyme solution (10 μg/ml, porcine pancreatic elastase standard: Porcine Pancreatic Elastase . 20 μl of the substrate solution, 20 μl of purified water, and 100 μl of the sample were added to 60 μl of the buffer solution, and the absorbance C was measured using the obtained solution as a control solution. In addition, the absorbance A is measured using the solution obtained by manipulation in the same way as the test solution with 100 μl of purified water instead of the sample as a blank test solution, and 120 μl of purified water is added instead of the enzyme solution and the sample and the solution obtained by manipulation in the same way as the test solution is color-coded. The absorbance D was measured as semen, and the elastase inhibition rate (%) was obtained according to Equation 1.

[Equation 1]

Elastase inhibition rate (%) = [1 - (enzyme activity of sample treatment group / enzyme activity of control group)] x 100

Table 2 below shows the elastase inhibitory activity in percentage.

As shown in Table 2, it was confirmed that the elastase inhibition rate of Examples 1-3 containing all 5 active ingredients was significantly higher compared to Comparative Examples 1 to 6 which did not contain some of the 5 active ingredients.

<Experimental Example 3> Sensory evaluation

After manufacturing the inner beauty jelly as shown in Table 3 below, sensory evaluation on color, scent and taste was performed for 30 adults in their 20s to 40s, and then, as a comprehensive preference, a 5-point scale method (1 point: very bad, 2 points: bad, 3 points: normal, 4 points: good, 5 points: very good) and shown in Table 3.

As shown in Table 3, it was confirmed that Examples 1-3 were significantly improved in overall acceptability compared to Comparative Examples 1 to 6.

Production example of health food

The active substance according to the present invention can be manufactured into various types of health food depending on the purpose. The following exemplifies methods for producing some health foods containing the active substances according to the present invention as active ingredients, but the present invention is not limited thereto.

<Health food manufacturing example 1> Manufacturing of dairy products

0.01-1 part by weight of the active substance of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health food manufacturing example 2> Manufacturing of food

Brown rice, barley, glutinous rice, and adlay were alphanized by a known method, dried, roasted, and then prepared into a powder having a particle size of 60 mesh using a grinder. Black beans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, roasted, and then prepared into powder with a particle size of 60 mesh by a grinder. The active substance of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. It was prepared by blending the dry powder of grains, seeds and active substances prepared above in the following ratio.

Cereals (brown rice 34 parts by weight, adlay 19 parts by weight, barley 20 parts by weight),

Seeds (7 parts by weight of perilla seeds, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds),

Active substance (2 parts by weight),

reishi (1.5 parts by weight), and

Rehmannia glutinosa (1.5 parts by weight).

Production example of cosmetics

The active substance according to the present invention can be manufactured into various types of cosmetics depending on the purpose. The following exemplifies a method for preparing some cosmetics containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Cosmetics Production Example 1> Production of softened lotion

Active substance 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium EDTA 0.05 parts by weight

Allantoin 0.10 part by weight

Dipotassium glycyrrhizate 0.05 parts by weight

Citric acid 0.01 parts by weight

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated castor oil 1.00 parts by weight

30.0 parts by weight of ethanol

trace amounts of preservatives

trace amount of colorant

traces of flavoring

Remaining amount of purified water

<Cosmetic preparation example 2> Manufacture of nutrient cream

Active substance 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

Glycerin 1.00 parts by weight

D-panthenol 0.10 part by weight

3.20 parts by weight of plant extract

Magnesium aluminum silicate 0.30 part by weight

1.20 parts by weight of PEG-40 stearate

2.00 parts by weight of stearic acid

Polysorbate 60 1.50 parts by weight

2.00 parts by weight of lipophilic glyceryl stearate

1.50 parts by weight of sorbitan sesquioleate

Cetearyl alcohol 3.00 parts by weight

Mineral oil 4.00 parts by weight

3.80 parts by weight of squalane

Carrylic/capric triglyceride 2.80 parts by weight

Vegetable oil 1.80 parts by weight

Dimethicone 0.40 parts by weight

Diposium glycyrrhizate trace amount

trace amounts of allantoin

trace amounts of sodium hyaluronate

Dosage of Tocopheryl Acetate

Triethanolamine dosage

appropriate amount of preservative

Appropriate amount of flavoring agent

Remaining amount of purified water

Production example of health functional food

The active substance according to the present invention can be manufactured into various types of health functional food depending on the purpose. The following exemplifies a method for producing some health functional foods containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health functional food manufacturing example 1> Manufacturing of health functional food

Active substance 100 mg

Appropriate amount of vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

Folic acid 50 μg

Calcium Pantothenate 0.5 mg

Appropriate amount of mineral mixture

Ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium Carbonate 25.3 mg

Potassium Phosphate Monobasic 15 mg

Dibasic Calcium Phosphate 55 mg

Potassium citrate 90 mg

Calcium Carbonate 100 mg

Magnesium Chloride 24.8 mg

Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for relatively health functional food in a preferred embodiment, the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a normal health functional food manufacturing method. Then, granules can be prepared and used in the preparation of health functional food compositions according to conventional methods.

<Health functional food manufacturing example 2> Manufacturing of health functional beverages

Active substance 100 mg

Citric Acid 100 mg

Oligosaccharides 100 mg

Plum concentrate 2 mg

Taurine 100 mg

Add purified water to total 500 mL

After mixing the above ingredients according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized container, sealed and sterilized, and then refrigerated according to the present invention. It is used in the manufacture of health beverage compositions. Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

So far, the present invention has been looked at with respect to its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is particularly indicated in the claims rather than the foregoing description, and all differences within the equivalent range should be construed as being included in the present invention.

Claims (8)

Based on 5 parts by weight of red sea cucumber concentrate;
4-6 parts by weight of fish collagen;
Green tangerine juice 19-21 parts by weight;
Beet juice 9-11 parts by weight; and
Citrus flower honey 0.9-1.1 parts by weight; including,
A health food composition for preventing or improving skin wrinkles.
delete According to claim 1,
The health food composition is a health food composition for preventing or improving skin wrinkles, characterized in that the inner beauty (inner beauty) food.
According to claim 1,
The health food composition is a health food composition for preventing or improving skin wrinkles, characterized in that in the form of a jelly.
Based on 59 parts by weight of jelly base,
Red sea cucumber concentrate 4-6 parts by weight,
4-6 parts by weight of fish collagen;
Green tangerine juice 19-21 parts by weight,
9-11 parts by weight of beet juice, and
A jelly composition comprising 0.9-1.1 parts by weight of citrus flower honey.
According to claim 5,
The jelly base is a jelly composition, characterized in that it comprises vitamin C, enzyme-treated stevia, elastin, purified water, fructooligosaccharide, chicory dietary fiber, locust bean gum, xanthan gum, glucose, agar and tamarind gum.
Based on 5 parts by weight of red sea cucumber concentrate;
4-6 parts by weight of fish collagen;
Green tangerine juice 19-21 parts by weight;
Beet juice 9-11 parts by weight; and
Citrus flower honey 0.9-1.1 parts by weight; including,
A cosmetic composition for preventing or improving skin wrinkles.
Based on 5 parts by weight of red sea cucumber concentrate;
4-6 parts by weight of fish collagen;
Green tangerine juice 19-21 parts by weight;
Beet juice 9-11 parts by weight; and
Citrus flower honey 0.9-1.1 parts by weight; including,
Health functional food composition for preventing or improving skin wrinkles.