KR101474998B1 - Composition comprising extracts of white tea for the care of skin wrinkle - Google Patents
Composition comprising extracts of white tea for the care of skin wrinkle Download PDFInfo
- Publication number
- KR101474998B1 KR101474998B1 KR1020120136469A KR20120136469A KR101474998B1 KR 101474998 B1 KR101474998 B1 KR 101474998B1 KR 1020120136469 A KR1020120136469 A KR 1020120136469A KR 20120136469 A KR20120136469 A KR 20120136469A KR 101474998 B1 KR101474998 B1 KR 101474998B1
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- KR
- South Korea
- Prior art keywords
- white tea
- extract
- tea extract
- skin
- ethanol
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- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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Abstract
본 발명은 인간의 피부섬유아세포에서 콜라겐 생합성 촉진 및 MMP-1 억제하는 활성을 가지고 있는 백차(white tea) 추출물이 유효성분으로 포함되어 있는 피부 주름 생성 억제 및 주름 개선용 화장품 또는 식품 조성물에 관한 것이다.
상기 백차(white tea) 추출물은 자외선 조사에 의한 피부 주름 생성을 억제하거나 생성된 주름을 개선하는데 특히 유용하다.The present invention relates to a cosmetic or food composition for inhibiting skin wrinkle formation and improving wrinkles in which a white tea extract having an activity of promoting collagen biosynthesis and inhibiting MMP-1 in human skin fibroblasts is contained as an active ingredient .
The white tea extract is particularly useful for inhibiting skin wrinkle formation by ultraviolet irradiation or for improving wrinkles produced.
Description
본 발명은 인간의 피부섬유아세포에서 콜라겐 생합성 촉진 및 MMP-1 억제 활성을 보유한 백차(white tea) 추출물이 유효성분으로 포함되어 있는 피부 주름 생성 억제 및 주름 개선용 화장품 또는 식품 조성물에 관한 것이다.
The present invention relates to a cosmetic or food composition for inhibiting skin wrinkle formation and improving wrinkles in which a white tea extract having promoted collagen biosynthesis and MMP-1 inhibitory activity is contained as an active ingredient in human skin fibroblasts.
피부 노화는 주름의 증가, 피부 탄력감소, 색소 침착 등 여러 형태로 나뉠 수 있다. 그 중 주름 증가의 원인은 크게 내인성 요인과 외인성 요인으로 구분할 수 있다. 주름 증가의 내인성 요인은 나이가 들어감에 따라 피부의 구조와 생리 기능이 환경 변화와는 무관하게 감퇴하는 것이고, 외인성 요인은 태양 광, 공해, 질환, 음주, 흡연 등의 외부 환경 요인에 의해 나타나는 것이다. Skin aging can be divided into various forms such as wrinkles increase, skin elasticity decrease, pigmentation. Among them, the cause of wrinkles increase can be classified into endogenous factors and extrinsic factors. The endogenous factors of wrinkle increase are that the structure and physiological function of the skin decline with age, regardless of environmental changes, and the extrinsic factors are caused by external environmental factors such as sunlight, pollution, disease, drinking and smoking .
특히 태양 광에 의한 노화를 광노화(photoaging)라고 하며, 광노화의 주요 원인인 자외선은 피부노화의 생리학적 및 형태학적 변화를 초래한다. 한편 피부에의 자외선 조사는 주름, 일광 화상, 면역 억제, 암과 같은 다양한 변화를 일으키며, 피부 결합조직에 주로 발생하는 광 손상은 광노화의 특징적인 형태이다. 자외선에 노출된 피부에서는 O2-, OH·, RO2·, H2O2와 같은 활성산소종(reactive oxygen species, ROS)이 다량 생성된다. 활성산소종은 생체 내에서 에너지 생산과 식균작용, 세포성장과 세포내 신호조절과 같은 긍정적인 역할을 하는 반면에, 핵산, 단백질, 지방산, 당류 등과 반응하여 산화적 손상을 초래하기도 한다. 그리고 이러한 산화적 손상의 영향으로 세포 대사 교란, 형태학적 및 초미세 구조 변화 등이 초래되어 피부 광노화로 이어진다. 그 중 피부의 주 구조 성분인 콜라겐의 변화 및 감소는 피부의 주름 및 탄력의 감소와 같은 임상적 변화의 주요인이라고 알려져 있으며, 자연 노화 및 광 노화 모두에서 관찰되고 있다.In particular, aging caused by sunlight is called photoaging, and ultraviolet rays, which is a main cause of photoaging, cause physiological and morphological changes of skin aging. On the other hand, ultraviolet irradiation on the skin causes various changes such as wrinkles, sunburn, immunosuppression, cancer, and the photic damage mainly occurring in skin connective tissue is a characteristic form of photoaging. In skin exposed to ultraviolet rays, reactive oxygen species (ROS) such as O 2 - , OH -, RO 2 - , and H 2 O 2 are produced in large amounts. Active oxygen species play a positive role in vivo in energy production, phagocytosis, cell growth and intracellular signal regulation, but they also react with nucleic acids, proteins, fatty acids, saccharides, etc., and cause oxidative damage. These oxidative damages lead to cellular metabolism disturbance, morphological and ultrastructural changes and so on, leading to skin photoaging. Among them, changes and decreases in collagen, which is the main structural component of skin, are known to be the main causes of clinical changes such as reduction of wrinkles and elasticity of the skin, and are observed in both natural aging and photoaging.
콜라겐은 피부 주름과 관련된 주요 피부조직 성분이며, 지방을 제외한 피부 전체 건조 중량의 77%, 진피섬유 성분의 90%를 차지하고 있는 단백질로서, 피부의 강도, 탄력성, 유연성을 유지할 수 있게 하는 기능을 부여한다. 한편 콜라겐 생성의 감소 또는 콜라겐 분해 효소인 타입 I 콜라게나제(Matrix Metalloproteinase-1, MMP-1)의 증가에 의한 분해 촉진으로 콜라겐 결핍 현상이 발생한다고 알려져 있다. 따라서 콜라겐의 합성 증진 및 분해 억제를 통한 주름 생성 억제 및 주름 개선 효과를 기대 할 수 있다.Collagen is a major skin tissue component related to skin wrinkles. It is a protein that accounts for 77% of the dry weight of the skin except the fat and 90% of the dermis fiber component. It provides the function to maintain the strength, resilience and flexibility of the skin. do. On the other hand, it is known that collagen deficiency phenomenon occurs due to reduction of collagen production or promotion of decomposition by increase of collagenase type I (Matrix Metalloproteinase-1, MMP-1). Therefore, it is expected that the effect of improving the synthesis of collagen and inhibiting the decomposition of wrinkles will be suppressed and the wrinkles will be improved.
한편, 백차(white tea)는 솜털이 덮인 차의 어린 싹을 닦거나 비비지 않고 그대로 건조시키고, 이를 약간 발효시켜 만든 차이다. 백차는 지방질을 제거하고, 소변을 순조롭게 하며 장을 깨끗하게 해주는 효능을 가지고 있으므로 당뇨환자들에게 좋고, 여름에 열을 내려주는 작용이 있다고 하여 한약재로도 사용되고 있다.On the other hand, white tea is a tea made by slightly drying fermented young roots without drying or rubbing them. White tea has the efficacy to remove fat, smooth urine and clean the intestine, so it is good for diabetics and it is used as a medicinal herb because it has a heat-releasing action in summer.
최근에 공개된 발명으로서 한국공개특허 제2004-0076103호에는 백차, 녹두, 백지, 백급, 사인, 승마, 정향, 천화분을 일정 함량비로 혼합 및 추출하여 얻은 '백아단'이 프리라디칼 소거, 지질과산화 활성 억제, 티로시나아제 활성 저해, 멜라닌 생성 억제 실험에서 유효한 효과를 나타냄을 확인하여, 백아단이 유효성분으로 포함된 피부 미백 및 피부노화 방지용 화장품 조성물이 개시되어 있다. 한국공개특허 제2004-0076103호는 백차를 포함한 8가지 천연물의 혼합 추출물을 유효성분으로 하고 있고, 또한 피부 노화의 원인 중에서도 색소 침착 억제 또는 개선을 통해 피부 미백(skin-whitening) 효과를 얻고자 하는 화장품이 개시되어 있을 뿐이다.As a recently disclosed invention, Korean Patent Laid-Open Publication No. 2004-0076103 discloses a method in which a mixture of white tea, mung bean, white rice, white rice, sane, horse riding, cloves and chrysanthemum, Inhibiting activities of tyrosinase activity and inhibition of melanin production, and has been disclosed as a cosmetic composition for skin whitening and skin aging prevention, wherein the whitening agent is included as an active ingredient. Korean Patent Laid-Open No. 2004-0076103 discloses a cosmetic composition comprising eight mixed extracts of natural products including white tea as an active ingredient. In addition, among the causes of skin aging, a skin-whitening effect is obtained by inhibiting or improving pigmentation Cosmetics are only disclosed.
이에 반하여, 본 발명은 백차(white tea) 추출물이 인간의 피부섬유아세포에서 콜라겐 생합성 촉진 및 MMP-1 억제 활성을 가지고 있음을 확인한 발명으로, 주름 생성 억제 및 개선을 위한 링클 케어(wrinkle-care)용 화장품 또는 식품 조성물을 개발함으로써 본 발명을 완성하기에 이르렀다.
On the contrary, the present invention is an invention that confirms that white tea extract has collagen biosynthesis promotion activity and MMP-1 inhibitory activity in human skin fibroblasts. It is a wrinkle-care agent for suppressing and improving wrinkle formation, The present invention has been completed.
본 발명은 주름 생성 억제 및 개선을 위한 링클 케어용 화장품 또는 식품 조성물을 제공하는데 그 목적이 있다.
It is an object of the present invention to provide a cosmetic or food composition for wrinkle care for suppressing and improving wrinkle formation.
상기 과제 해결을 위하여, 본 발명은 백차(White tea) 추출물을 유효성분으로 함유하는 주름 생성 억제 및 주름 개선용 화장품 조성물을 특징으로 한다.In order to solve the above problems, the present invention is a cosmetic composition for suppressing wrinkle formation and improving wrinkles containing an extract of White tea as an active ingredient.
또한, 본 발명은 백차(White tea) 추출물을 유효성분으로 함유하는 주름 생성 억제 및 주름 개선용 식품 조성물을 특징으로 한다.
Further, the present invention is characterized by a food composition for suppressing wrinkle formation and improving wrinkles containing an extract of White tea as an active ingredient.
본 발명이 유효성분으로 포함하는 백차(white tea) 추출물은 인간의 피부섬유아세포에서 콜라겐 생합성 촉진 및 MMP-1 억제하는 활성이 우수하다.The white tea extract containing the present invention as an active ingredient has excellent activity of promoting collagen biosynthesis and inhibiting MMP-1 in human dermal fibroblasts.
따라서, 백차(white tea) 추출물은 피부 주름 생성 억제 또는 주름 개선을 목적으로 하는 링클 케어(wrinkle-care) 제품의 유효성분으로 유용하다.
Therefore, white tea extract is useful as an active ingredient of a wrinkle-care product for the purpose of suppressing the formation of wrinkles or improving the wrinkles of the skin.
도 1은 MTT Assay를 통해 UV damage를 받은 섬유아세포의 재생 효과를 비교한 차트이다.
도 2는 UV damage를 받은 섬유아세포의 MPP-1 발현량을 측정하여 시료의 MMP-1 억제율을 비교한 차트이다.
도 3은 UV damage를 받은 섬유아세포의 Type-1 Procollagen 생합성량을 측정하여 시료의 콜라겐 생합성량을 비교한 차트이다.FIG. 1 is a chart comparing the regeneration effect of fibroblasts subjected to UV damage through MTT assay.
FIG. 2 is a chart comparing the MMP-1 inhibition rate of a sample by measuring the expression level of MPP-1 in fibroblasts receiving UV damage.
FIG. 3 is a chart comparing the amount of collagen biosynthesis of a sample by measuring the amount of Type-1 Procollagen biosynthesis of fibroblasts that have been exposed to UV damage.
본 발명의 백차(white tea) 추출물은 백차를 건조 무게대비 5 내지 20 배, 바람직하게는 8 내지 10배 배 중량비의 용매로 추출하여 얻은 추출물이다. 본 발명에서 사용되는 추출용매는 정제수 및 통상의 유기용매로부터 선택된 단일 용매 또는 2종 이상의 혼합용매를 사용할 수 있다. 구체적으로는 정제수, 탄소수 1 내지 6의 알콜, 글리세린, 탄소수 2 내지 6의 알킬렌글리콜, 탄소수 1 내지 6의 알칸, 탄소수 1 내지 6의 할로겐화탄화수소, 탄소수 2 내지 6의 알킬 알카노에이트 및 그의 혼합물로부터 선택된 추출용매를 사용할 수 있다. 좀 더 구체적으로 예시하면 정제수, 메탄올, 에탄올, 글리세린, 에틸렌글리콜, 부틸렌글리콜, 프로필렌글리콜, 디클로로메탄, 디클로로에탄, 헥산, 에틸아세테이트 및 그의 혼합물로부터 선택된 추출용매를 사용할 수 있다. 특히 좋기로는 추출용매로서 50 내지 100 부피% 농도의 에탄올을 사용하는 것이다. The white tea extract of the present invention is an extract obtained by extracting a white tea with a solvent at a weight ratio of 5 to 20 times, preferably 8 to 10 times the dry weight. The extraction solvent used in the present invention may be a single solvent or a mixed solvent of two or more selected from purified water and common organic solvents. Specific examples thereof include purified water, alcohols having 1 to 6 carbon atoms, glycerin, alkylene glycols having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkylalkanoates having 2 to 6 carbon atoms, and mixtures thereof Can be used. More specifically, extraction solvents selected from purified water, methanol, ethanol, glycerin, ethylene glycol, butylene glycol, propylene glycol, dichloromethane, dichloroethane, hexane, ethyl acetate and mixtures thereof can be used. Particularly preferably, ethanol is used in an amount of 50 to 100% by volume as an extraction solvent.
상기 추출물은 20℃ 내지 200℃ 온도에서 30분 내지 20시간 동안 추출하여 수득할 수 있고, 좋기로는 상온(20℃ 내지 30℃)에서 1 내지 3일 동안 추출하여 수득할 수 있다. 상기 추출용매로 추출한 후에는 냉침 및 여과하고, 그 여액을 50℃ 이하에서 감압농축하고 감압농축물을 동결 건조하여 분말 형태로 추출물을 얻을 수 있다. 필요하다면, 상기 동결 건조한 추출물 분말을 물 또는 통상의 유기용매에 분산 또는 용해시켜 액체 형태로 얻을 수도 있다.The extract can be obtained by extraction at a temperature of 20 ° C to 200 ° C for 30 minutes to 20 hours, preferably by extraction at room temperature (20 ° C to 30 ° C) for 1 to 3 days. After extracting with the extraction solvent, it is cooled and filtered, and the filtrate is concentrated under reduced pressure at 50 ° C or less. The concentrated extract is freeze-dried to obtain an extract in powder form. If necessary, the lyophilized extract powder may be dispersed or dissolved in water or a conventional organic solvent to obtain a liquid form.
한편, 본 발명에 따른 화장품 조성물에 포함되는 백차 추출물의 함량은 화장품 조성물 전체에 대하여 동결건조중량 기준 0.001 내지 30.0 중량% 범위가 바람직하다. 상기 추출물의 함량이 0.001 중량% 미만인 경우에는 피부 주름 생성 억제 및 주름 개선 효과가 거의 없으며, 30.0 중량%를 초과하는 경우에는 함유량 증가에 대한 효과 증대 정도가 미미하여 경제적이지 못하기 때문이다.On the other hand, the content of the white tea extract contained in the cosmetic composition according to the present invention is preferably in the range of 0.001 to 30.0% by weight based on the freeze-dried weight of the whole cosmetic composition. If the content of the extract is less than 0.001% by weight, the skin wrinkle formation and wrinkle-reducing effects are hardly obtained. If the content is more than 30.0% by weight, the effect of increasing the content of the extract is insignificant and not economical.
또한 본 발명에 따른 화장품 조성물은 백차 추출물 이외에 본 발명이 목적으로 하는 주효과를 손상시키지 않는 범위 내에서 바람직하게는 주효과에 상승효과를 줄 수 있는 다른 성분 예를 들면, 녹차 추출물, 닥나무 추출물, 감초추출물, 상백피 추출물, 빈랑자 추출물, 황금 추출물, 산삼 추출물 등의 천연물을 함유하는 것도 무방하다.In addition, the cosmetic composition according to the present invention may contain, in addition to the white tea extract, other components which can give a synergistic effect to the main effect, for example, green tea extract, mulberry extract, Licorice extract, mulberry bark extract, bingo extract, golden extract, wild ginseng extract, and the like.
본 발명의 화장품 조성물은 수성, 수성-알콜성 또는 오일성 용액, 수중유 또는 유증수 또는 다중 에멀전, 수성 또는 오일성 겔, 액체, 페이스트성 또는 고체 무수 생성물, 소구체를 사용한 수성상에서의 오일분산물의 형태일 수 있으며 보다 바람직하게는 이온성 및/또는 비이온성 형태의 지질 소포체의 형태일 수 있다.The cosmetic compositions of the present invention may be in the form of an oil dispersion in an aqueous phase using an aqueous, aqueous-alcoholic or oily solution, oil or beer or multiple emulsion, aqueous or oily gel, liquid, pasty or solid anhydrous products, And more preferably in the form of a lipid vesicle in ionic and / or non-ionic form.
본 발명의 화장품 조성물은 다소 유체일 수 있으며, 백색 또는 유색크림, 연고, 밀크로션, 유액(serum), 에센스, 페이스트 또는 무스의 외관을 가질 수 있다. 이는 선택적으로 에어로졸 형태로 피부에 적용될 수도 있고, 고체 형태 예컨대, 스틱의 형태일 수도 있다. 이러한 백차 추출물을 포함하는 화장품 조성물은 화장수, 스킨, 로션, 영양로션, 엣센스, 영양크림, 마사지 크림, 유화형 화운데이션, 또는 팩 형태로 제형화하여 피부 케어 제품 및/또는 메이크업 제품으로서 사용될 수도 있다.The cosmetic composition of the present invention may be somewhat fluid and may have the appearance of a white or colored cream, ointment, milk lotion, serum, essence, paste or mousse. It may optionally be applied to the skin in aerosol form, or it may be in solid form, e.g., in the form of a stick. The cosmetic composition containing such white tea extract may be used as a skin care product and / or a makeup product by formulating it in the form of lotion, skin, lotion, nutrition lotion, essence, nutritional cream, massage cream, emulsion type foundation or pack.
본 발명의 화장품은 당 분야에서 주름 생성 억제 또는 주름 개선에 유효한 것으로 알려진 통상의 성분으로서 예를 들면 수용성 비타민, 지용성 비타민, 고분자 펩티드, 고분자 다당, 스핑고 지질, 해초 엑기스 등을 추가로 포함할 수 있다. 수용성 비타민은 화장품에 배합 가능한 것으로 비타민 B1, 비타민 B2, 비타민 B6, 피리독신, 염산피리독신, 비타민 B12, 판토텐산, 니코틴산, 니코틴산아미드, 엽산, 비타민 C, 비타민 H 등이 포함될 수 있고, 이들의 염이나 유도체도 사용될 수 있다. 지용성 비타민은 화장품에 배합 가능한 것으로 비타민 A, 카로틴, 비타민 D2, 비타민 D3, 비타민 E (dℓ-알파 토코페롤, d-알파 토코페롤) 등이 포함될 수 있고, 이들의 염이나 유도체도 사용될 수 있다. 고분자 펩티드는 화장품에 배합 가능한 것으로 콜라겐, 가수 분해 콜라겐, 젤라틴, 엘라스틴, 가수 분해 엘라스틴, 케라틴 등이 포함될 수 있다. 고분자 다당은 화장품에 배합 가능한 것으로 히드록시에틸셀룰로오스, 크산탄검, 히알루론산나트륨, 콘드로이틴 황산 등이 포함될 수 있고, 이들의 염도 사용될 수 있다. 스핑고 지질은 화장품에 배합 가능한 것으로 세라미드, 피토스핑고신, 스핑고당지질 등이 포함될 수 있다. 해초 엑기스는 화장품에 배합 가능한 것으로 갈조 엑기스, 홍조 엑기스, 녹조 엑기스 등이 포함될 수 있고, 이들의 해초 엑기스로부터 정제된 칼라기난, 아르긴산, 아르긴산나트륨, 아르긴산칼륨 등도 포함될 수 있다.The cosmetics of the present invention may further contain water-soluble vitamins, lipid-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids, seaweed extracts, etc. as common components known to be effective for suppressing wrinkle formation or improving wrinkles in the art have. The water-soluble vitamin may be formulated in cosmetics and may include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H, Can also be used. The fat-soluble vitamins can be formulated in cosmetics and include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (dl-alpha tocopherol, d-alpha tocopherol), and their salts and derivatives. Polymer peptides can be formulated into cosmetics and include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. The polymer polysaccharide may be incorporated in cosmetics and may include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate, and the like, and salts thereof may also be used. Sphingo lipids can be formulated in cosmetics and may include ceramides, phytosphingosine, sphingoglycolipids, and the like. The seaweed extract can be incorporated in cosmetics, and may include a brown algae extract, a red algae extract, a green algae extract, and the like, and may contain coloring guanine, arginic acid, sodium alginate, and potassium alginate refined from seaweed extract.
또한, 본 발명의 화장품 조성물은 화장 분야에서 통상적으로 사용되는 보조제로서 예를 들면 친수성 또는 친지성 겔화제, 친수성 또는 친지성 활성제, 보존제, 항산화제, 용매, 방향제, 충전제, 차단제, 안료, 흡취제 및 염료를 추가로 함유할 수 있다. 이들 다양한 보조제의 양은 당해 분야에서 통상적으로 사용되는 양이며, 예컨대 화장품 조성물 총중량에 대해 0.01 내지 20 중량% 범위이다. 그 성질에 따라 이러한 보조제는 지방상, 수성상, 지질 소포체 및/또는 나노 입자에 도입될 수 있다. 어떠한 경우라도 보조제 및 그 비율은 본 발명에 따른 화장품 조성물의 바람직한 성질에 악영향을 미치지 않도록 선택될 것이다.
Also, the cosmetic composition of the present invention is an adjuvant commonly used in cosmetics, for example, a hydrophilic or lipophilic gelling agent, a hydrophilic or lipophilic active agent, a preservative, an antioxidant, a solvent, a perfume, a filler, And a dye. The amount of these various adjuvants is an amount ordinarily used in the art, for example, in the range of 0.01 to 20% by weight based on the total weight of the cosmetic composition. Depending on their nature, such adjuvants can be introduced into the lipid, aqueous phase, lipid vesicles and / or nanoparticles. In any case, the adjuvants and their proportions will be selected so as not to adversely affect the desired properties of the cosmetic compositions according to the invention.
한편, 본 발명에 따른 식품 조성물은 백차 추출물을 유효성분으로 포함시켜 분말제, 액제, 정제, 연질캅셀제, 과립제, 티백차, 인스턴트 차 또는 드링크제 등의 형태로 제형화될 수 있다. 유효 성분으로서의 백차 추출물의 함량은 그 사용 목적(예방 또는 개선용)에 따라 적합하게 결정될 수 있다. 일반적으로, 식품 조성물 중에 포함되는 백차 추출물의 양은 전체 식품 중량의 0.1 내지 90 중량%로 가할 수 있다. 그러나 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로도 사용될 수 있다. 또한, 본 발명에 따른 식품 조성물은 백차 추출물 이외에 본 발명이 목적으로 하는 주효과를 손상시키지 않는 범위 내에서 바람직하게는 주효과에 상승효과를 줄 수 있는 다른 성분 예를 들면, 녹차 추출물, 닥나무 추출물, 감초추출물, 상백피 추출물, 빈랑자 추출물, 황금 추출물, 산삼 추출물 등의 천연물을 함유하는 것도 무방하다.Meanwhile, the food composition according to the present invention can be formulated in the form of a powder, a liquid, a tablet, a soft capsule, a granule, a tea bag, an instant tea or a drink by including a white tea extract as an active ingredient. The content of the white tea extract as an active ingredient can be suitably determined according to the intended use (for prevention or improvement). Generally, the amount of the white tea extract contained in the food composition may be 0.1 to 90% by weight of the total food weight. However, in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range. In addition, the food composition according to the present invention may contain, in addition to the white tea extract, other ingredients which can give a synergistic effect to the main effect, such as green tea extract, , Licorice extract, mulberry bark extract, bingo extract, golden extract, wild ginseng extract, and the like.
상기와 같은 형태로 제형화된 식품 조성물은 식품에 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 식품의 예로는 드링크제, 육류, 소시지, 빵, 비스킷, 떡, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 알코올 음료 및 비타민 복합제, 유제품 및 유가공 제품 등이 있으며, 통상적인 의미에서의 건강기능식품은 모두 포함한다. The food composition formulated in this form can be added directly to the food or used together with other food or food ingredients, and can be suitably used according to conventional methods. Examples of food products include dairy products including drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, soups, , Dairy products and dairy products, and all health functional foods in the ordinary sense are included.
본 발명의 식품 조성물이 드링크제인 경우는 지시된 비율로 필수성분으로서 백차 추출물을 함유하며, 그 밖의 드링크제 제조를 목적으로 사용되는 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상기한 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스 등; 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 상술한 것 이외의 향미제로서 천연 향미제 및 합성 향미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖당 일반적으로 약 1 내지 20 g, 바람직하게는 약 5 내지 12 g이다.When the food composition of the present invention is a drink, it contains the white tea extract as an essential ingredient at the indicated ratio. There are no particular restrictions on other ingredients used for the purpose of preparing other drink products, and various flavors or natural Carbohydrates and the like as additional components. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors and synthetic flavors and the like can be used as the flavors other than those described above. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.
또한 본 발명의 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에도 본 발명의 본 발명의 식품 조성물은 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 그렇게 중요하진 않지만 본 발명의 백차 추출물 100 중량부 당 0.1 내지 약 20 중량부의 범위에서 선택되는 것이 일반적이다.In addition, the food composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the inventive food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the white tea extract of the present invention.
이상에서 설명한 바와 같은 본 발명은 하기의 실시예에 의거하여 더욱 상세히 설명하겠는 바, 본 발명이 이에 한정되는 것은 아니다.
The present invention will now be described in more detail with reference to the following examples, but the present invention is not limited thereto.
[실시예]
[Example]
제조예 : 백차 추출물의 제조Production Example: Preparation of White Tea Extract
백차 건조 잎은 중국 복건성지방의 생산품을 이용하였고, 백차의 등급 중 백모단과 수미를 이용하였다. 백차 건조 잎은 완전히 분쇄하여 추출에 사용하였다. 추출용매로는 50% 에탄올 수용액을 사용하였고, 백차 건조 잎의 무게 대비로 10 중량배를 사용하였다. 40℃에서 교반하면서 가용성 성분을 3시간 정도 추출하였다. 추출물은 여과 및 감압 농축한 후에, 동결건조하여 분말 형태의 백차 추출물을 얻었다.
Dried leaves of white tea were made by Fujian province of China, white tea and white tea were used in white tea grade. Dried leaves of white tea were completely pulverized and used for extraction. A 50% aqueous ethanol solution was used as the extraction solvent, and 10 times the weight of the dried leaf of the white tea was used. The soluble components were extracted for about 3 hours while stirring at 40 占 폚. The extract was filtered and concentrated under reduced pressure, and then lyophilized to obtain a white tea extract in powder form.
또한, 백차 추출물과의 효능 비교를 위하여 비교군으로서 녹차, 핑크로즈, 로즈마리, 마테차를 각각 상기 제조예의 방법으로 추출하여 비교추출물을 얻었다.
In order to compare the efficacy with the white tea extract, green tea, pink rose, rosemary, and matéa were respectively extracted as comparative groups to obtain comparative extracts.
실시예 1 : MTT Assay(cell proliferation 실험)Example 1: MTT assay (cell proliferation experiment)
MTT assay를 통해 시료와 조사되는 UV의 양에 따른 세포 독성을 측정함으로써 본 실험에 사용되는 적당한 시료의 양과 조사되는 UV의 양을 결정하고, 또한 처리된 시료가 세포의 생장에 어떠한 영향을 주는지 비교하였다.The MTT assay was used to determine the amount of UV-irradiated sample and the amount of UV used in this experiment by measuring the cytotoxicity of the sample and the amount of irradiated UV, Respectively.
본 실험에서 사용되는 인간의 피부 섬유아세포(Fibroblast, HS-68)를 이산화탄소 인큐베이터(37℃, CO2 5%)에서 85%이상 키운 후 24 웰 플레이트에 1.25x105 cells/mL 농도다 되도록 고르게 분주하였다. 24시간이 지난 후 각 웰을 인산완충액(PBS)으로 세척하고, 시료를 농도별로 녹인 무세럼 배지를 1 mL 분주하였다. 24시간 배양한 후 각 웰을 PBS로 세척하고, UV 크로스링커를 이용하여 UV를 20 mJ/cm2 조사하였다. 다시 각 웰 플레이트에 시료를 농도별로 녹인 무세럼 배지를 분주하여 24시간 배양하였다. 각 웰을 다시 PBS로 세척한 후, PBS에 용해시킨 MTT(Dimethyl thiazolyl diphenyl tetrazolium salt)를 0.5 mg/mL의 농도로 각 웰당 500 uL씩 넣었다. 이산화탄소 인큐베이터에서 4시간 반응시키고, 96 웰 플레이트에 디메틸설폭사이드(DMSO)를 이용하여 적당히 희석한 후 ELISA에서 540 nm 파장으로 흡광도를 측정하였다. 세포 생존율(Cell survival rate)은 하기 수학식 1에 의해 계산하였다. 그 결과는 도 1에 나타내었다.The human skin fibroblast (HS-68) used in this experiment was raised to 85% or more in a carbon dioxide incubator (37 ° C, 5% CO 2 ), and then added to a 24-well plate at a concentration of 1.25 × 10 5 cells / Respectively. After 24 hours, each well was washed with phosphate buffer (PBS), and 1 mL of non-serum medium in which the sample was dissolved by concentration was dispensed. After incubation for 24 hours, each well was washed with PBS and irradiated with UV at 20 mJ / cm 2 using a UV cross linker. Then, the non-serum medium in which the samples were dissolved by concentration was dispensed into each well plate and cultured for 24 hours. After washing each well with PBS, 500 μL of MTT (dimethyl thiazolyl diphenyl tetrazolium salt) dissolved in PBS was added at a concentration of 0.5 mg / mL to each well. The reaction was carried out in a carbon dioxide incubator for 4 hours. After appropriately diluting with dimethylsulfoxide (DMSO) in a 96-well plate, the absorbance was measured by ELISA at a wavelength of 540 nm. The cell survival rate was calculated by the following equation (1). The results are shown in Fig.
[수학식 1][Equation 1]
도 1의 결과에 의하면, UV 조사된 섬유아세포의 재생과 회복에는 마테차 추출물이 가장 높게 나왔으며 핑크로즈 추출물과 백차 추출물는 큰 차이 없이 세포 재생 및 회복에 효과가 있음을 알 수 있다. 세포의 재생과 회복은 주름 생성 억제 및 주름 개선에 효과에 영향을 줄 것으로 예측되나, 정확한 링클 케어 효능을 확인하기 위하여 하기 실시예 2와 실시예 3에 의거하여 콜라겐 분해 효소인 MMP-1의 발현량과 콜라겐의 생성량을 비교하였다.The results of FIG. 1 show that the matte extract is the highest in the regeneration and recovery of the UV-irradiated fibroblasts, and that the pink rose extract and the white tea extract are effective for cell regeneration and recovery without significant difference. Regeneration and restoration of the cells are expected to affect the effect of suppressing wrinkle formation and improving wrinkles. However, in order to confirm the accurate Wrinkle care effect, according to Example 2 and Example 3, the collagenase enzyme MMP- The amount of collagen produced was compared with the amount of collagen.
실시예 2 : MMP-1 억제 효능 실험Example 2: MMP-1 inhibitory activity test
본 실험에서 사용되는 인간의 피부 섬유아세포(Fibroblast, HS-68)는 상기 실시예 1의 방법으로 배양하였다. MMP-1 발현량 측정은 머크(MERCK)사의 QIA55 MMP-1 ELISA Kit 장비를 사용하였다. 측정된 MMP-1 억제효능 결과는 도 2에 나타내었다.The human skin fibroblast (Fibroblast, HS-68) used in this experiment was cultured by the method of Example 1 above. MMP-1 expression level was measured using a QIA55 MMP-1 ELISA Kit from Merck. The measured MMP-1 inhibitory effect is shown in FIG.
도 2의 결과에 의하면, 백차 추출물이 섬유아세포에서의 MMP-1 저해율이 가장 우수하였다. 이로써 백차 추출물의 콜라겐 분해를 억제하는 효능이 가장 우수함을 확인할 수 있었다. 백차 추출물의 MMP-1 저해율은 녹차 추출물, 핑크로즈 추출물, 로즈마리 추출물, 마테차 추출물에 비해 거의 2배 가량 높게 나왔다.
As shown in Fig. 2, the inhibition of MMP-1 in fibroblasts was the most excellent for the white tea extract. Thus, it was confirmed that the effect of suppressing the collagen decomposition of the white tea extract was excellent. The inhibition of MMP-1 in white tea extract was almost twice as high as that of green tea extract, pink rose extract, rosemary extract and matée extract.
실시예 3 : 콜라겐 생합성 효능 실험Example 3: Effect of collagen biosynthesis
본 실험에서 사용되는 인간의 피부 섬유아세포(Fibroblast, HS-68)는 상기 실시예 1의 방법으로 배양하였다. Type-1 Procollagen 발현량 측정은 타카라(TAKARA)사의 Procollagen Type 1 C-Peptide(PIP) EIA Kit를 사용하였다. 측정된 Type-1 Procollagen 증가효능 결과는 도 3에 나타내었다.The human skin fibroblast (Fibroblast, HS-68) used in this experiment was cultured by the method of Example 1 above. The expression level of Type-1 Procollagen was measured using a Procollagen Type 1 C-Peptide (PIP) EIA Kit from Takara. The measured Type-1 Procollagen increasing efficacy results are shown in FIG.
도 3의 결과에 의하면, MMP-1 저해율 실험을 통해 예측한 바와 같이 백차 추출물이 콜라겐 생합성 효능이 가장 우수함을 확인할 수 있었다.
As can be seen from the results of FIG. 3, it was confirmed that the white tea extract had the best collagen biosynthesis efficiency as predicted by the MMP-1 inhibition experiment.
[제제예]
[Formulation Example]
또한, 본 발명에 따른 백차 추출물을 포함하는 화장품 조성물은 목적에 따라 여러 형태로 제조가 가능하다. 하기 제제 1 내지 4는 본 발명에 따른 백차 추출물을 유효성분으로 함유하는 화장품 제조방법을 예시한 것으로 본 발명이 이에 한정되는 것은 아니다.
The cosmetic composition containing the white tea extract according to the present invention may be prepared in various forms depending on the purpose. The following Formulation Examples 1 to 4 illustrate a method for producing cosmetics containing the white tea extract according to the present invention as an active ingredient, but the present invention is not limited thereto.
제제 1: 화장용 스킨Formulation 1: Cosmetic Skin
백차 추출물 0.0018 mg, 히드록시에틸셀룰로오스 (2% 수용액) 12 mg, 크산탄검 (2% 수용액) 2 mg, 1,3-부틸렌글리콜 6 mg, 진한 글리세린 4 mg, 히알루론산나트륨 (1% 수용액) 5 mg을 적당량의 정제수에서 혼합하여 화장용 스킨을 제조하였다.
(2% aqueous solution), 2 mg of xanthan gum (2% aqueous solution), 6 mg of 1,3-butylene glycol, 4 mg of concentrated glycerin, 4 g of sodium hyaluronate (1% aqueous solution ) Were mixed in an appropriate amount of purified water to prepare a cosmetic skin.
제제 2: 화장용 로션Formulation 2: Lotion for cosmetics
백차 추출물 0.0015 mg, L-아스코르빈산-2-인산마그네슘염 1 mg, 수용성 콜라겐 (1% 수용액) 1 mg, 시트르산나트륨 0.1 mg, 시트르산 0.05 mg, 감초 엑기스 0.2 mg, 1,3-부틸렌글리콜 3 mg을 적당량의 정제수에서 혼합하여 화장용 로션을 제조하였다.
(1% aqueous solution), 0.1 mg of sodium citrate, 0.05 mg of citric acid, 0.2 mg of licorice extract, 0.1 mg of 1,3-butylene glycol 3 mg were mixed in an appropriate amount of purified water to prepare a cosmetic lotion.
제제 3: 화장용 크림Formulation 3: Cosmetic cream
백차 추출물 0.0012 mg에 폴리에틸렌글리콜모노스테아레이트 2 mg, 자기유화형 모노스테아르산글리세린 5 mg, 스쿠알렌 6 mg, 트리2-에틸헥산산글리세릴 6 mg, 스핑고당지질 1 mg, 1,3-부틸렌글리콜 7 mg을 적당량의 정제수에서 혼합하여 화장용 크림을 제조하였다.
2 mg of polyethylene glycol monostearate, 5 mg of self-emulsifying monostearate glycerin, 6 mg of squalane, 6 mg of tri-2-ethylhexanoic acid glyceryl, 1 mg of sphingoglycolipid, 1 mg of 1,3-butylene 7 mg of glycol were mixed in an appropriate amount of purified water to prepare a cosmetic cream.
제제 4: 화장용 마사지 크림Formulation 4: Cosmetic massage cream
백차 추출물 1 mg에 식물성 경화유 1.5 mg, 스테아린산 0.6 mg, 스테아릴 알코올 2 mg, 글리세롤 스테아레이트 1 mg, 프로필렌글리콜 1.5 mg, 아라키딜글루코사이드 1 mg, 카프릴릭 5 mg, 사이클로메디콘 4 mg, 트리에탄올아민 0.1 mg, 스쿠알란 2.5 mg을 적당량의 정제수에서 혼합하여 화장용 마사지 크림을 제조하였다.To 1 mg of white tea extract were added 1.5 mg of vegetable hydrogenated oil, 0.6 mg of stearic acid, 2 mg of stearyl alcohol, 1 mg of glycerol stearate, 1.5 mg of propylene glycol, 1 mg of arachidyl glucoside, 5 mg of caprylic, 4 mg of cyclomedicone, 0.1 mg of amine and 2.5 mg of squalane were mixed in an appropriate amount of purified water to prepare a cosmetic massage cream.
또한, 본 발명에 따른 백차 추출물을 포함하는 식품 조성물은 목적에 따라 여러 형태로 제조가 가능하다. 하기 제제 5 내지 9는 본 발명에 따른 백차 추출물을 유효성분으로 함유하는 식품 제조방법을 예시한 것으로 본 발명이 이에 한정되는 것은 아니다.
In addition, the food composition containing the white tea extract according to the present invention may be prepared in various forms depending on the purpose. The following Preparations 5 to 9 exemplify the method for producing a food containing the white tea extract according to the present invention as an active ingredient, but the present invention is not limited thereto.
제제 5. 과립상 건강식품Formulation 5. Granular health food
백차 추출물 1000 ㎎, 비타민 A 아세테이트 70 ㎍, 비타민 E 1.0 ㎎, 비타민 B1 0.13 ㎎, 비타민 B2 0.15 ㎎, 비타민 B6 0.5 ㎎, 비타민 B12 0.2 ㎍, 비타민 C 10 ㎎, 비오틴 10 ㎍, 니코틴산아미드 1.7 ㎎, 엽산 50 ㎍, 판토텐산 칼슘 0.5 ㎎, 황산제1철 1.75 ㎎, 산화아연 0.82 ㎎, 탄산마그네슘 25.3 ㎎, 제1인산칼륨 15 ㎎, 제2인산칼슘 55 ㎎, 구연산칼륨 90 ㎎, 탄산칼슘 100 ㎎, 염화마그네슘 24.8 ㎎을 혼합한 다음, 통상의 방법에 따라 과립상 건강식품을 제조하였다.
White tea extract 1000 ㎎, vitamin A acetate 70 ㎍, vitamin E 1.0 ㎎, Vitamin B 1 0.13 ㎎, Vitamin B 2 0.15 ㎎, vitamin B 6 0.5 ㎎, vitamin B 12 0.2 ㎍, vitamin C 10 ㎎, biotin 10 ㎍, nicotinic acid Amide 1.7 mg,
제제 6. 음료Formulation 6. Drink
백차 추출물 1000 ㎎, 구연산 1000 ㎎, 올리고당 100 g, 매실농축액 2 g, 타우린 1 g을 혼합하고, 여기에 정제수를 가하여 전체 용량을 900 ㎖로 조절하였다. 약 1시간 동안 85℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2ℓ 용기에 취득하여 밀봉 멸균하여 건강음료를 제조하였다.
1000 mg of white tea extract, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate and 1 g of taurine were mixed, and the total volume was adjusted to 900 ml by adding purified water thereto. After stirring and heating at 85 DEG C for about 1 hour, the solution thus prepared was filtered and sterilized in a 2-liter sterile container to prepare a health drink.
상기 조성비는 비교적 기호 음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만 수요계층이나, 수요국가, 사용용도 등 지역적, 민족적 기호 도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.
Although the composition ratio is relatively mixed with the ingredient suitable for the favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand class, demand country, use purpose, and the like.
제제 7. 밀가루 식품Formulation 7. Flour Food
백차 추출물 0.5 내지 5 g을 밀가루 100 g에 첨가하고, 이 혼합물을 이용하여 빵, 케이크, 쿠키, 크래커 및 면류를 제조하여 건강 증진용 식품을 제조하였다.
0.5 to 5 g of white tea extract was added to 100 g of wheat flour and the mixture was used to prepare breads, cakes, cookies, crackers and noodles for health promotion.
제제 8. 유제품Formulation 8. Dairy products
백차 추출물 10 g을 우유 100 g에 첨가하고, 상기 우유를 이용하여 버터 및 아이스크림과 같은 다양한 유제품을 제조하였다.
10 g of the white tea extract was added to 100 g of milk and various dairy products such as butter and ice cream were prepared using the milk.
제제 9. 선식Formulation 9.
현미 30 g, 보리 20 g, 찹쌀 10 g, 율무 15 g을 공지의 방법으로 알파화 시켜서 건조한 것을 배전한 후 분쇄기로 입도 60 메시의 분말로 제조하였다. 검은콩 7 g, 검정깨 7 g, 들깨 7 g을 공지의 방법으로 쪄서 건조한 것을 배전한 후 분쇄기로 입도 60 메시의 분말로 제조하였다. 상기에서 제조한 곡물류 및 종실류와 백차 추출물 3 g을 혼합하여 선식을 제조하였다.
30 g of brown rice, 20 g of barley, 10 g of glutinous rice, and 15 g of yulmu were alphalized by a known method and dried, and the powder was prepared into a powder having a particle size of 60 mesh by a pulverizer. 7 g of black beans, 7 g of black sesame, and 7 g of perilla were steamed and dried in a known manner, and the resulting mixture was used as a powder having a particle size of 60 mesh. The seeds and seeds prepared above and 3 g of the white tea extract were mixed to prepare an electric wire.
이상에서 설명한 바와 같이, 백차 추출물은 콜라겐 생합성 촉진 활성과 MMP-1 억제 활성이 우수함을 확인할 수 있다.As described above, it can be confirmed that the white tea extract has excellent collagen biosynthesis promoting activity and MMP-1 inhibitory activity.
따라서, 백차 추출물은 피부 주름 생성 억제 및 주름 개선을 목적으로 하는 화장품 또는 식품의 활성성분으로 유용하다.Therefore, the white tea extract is useful as an active ingredient in cosmetics or food for the purpose of inhibiting wrinkle formation and improving wrinkles.
Claims (10)
A cosmetic composition for inhibiting wrinkle formation and improving wrinkles containing an effective amount of a white tea extract obtained by extracting white tea with ethanol or an aqueous solution of ethanol as an active ingredient and having an activity of promoting collagen biosynthesis and inhibiting MMP-1.
상기 백차(White tea) 추출물은 50 ~ 100% 에탄올 추출물인 것을 특징으로 하는 주름 생성 억제 및 주름 개선용 화장품 조성물.
The method of claim 1, wherein
Wherein the white tea extract is 50 ~ 100% ethanol extract.
상기 백차(White tea) 추출물은 화장수, 스킨, 로션, 영양로션, 엣센스, 영양크림, 마사지 크림, 유화형 화운데이션, 또는 팩 형태로 제형화된 것을 특징으로 하는 주름 생성 억제 및 주름 개선용 화장품 조성물.
3. The method according to claim 1 or 2,
Wherein the white tea extract is formulated in the form of lotion, skin, lotion, nutrition lotion, essence, nutritional cream, massage cream, emulsion type foundation, or pack.
A white tea extract obtained by extracting white tea with an ethanol or ethanol aqueous solution as an active ingredient and having an activity of promoting collagen biosynthesis and inhibiting MMP-1.
상기 백차(White tea) 추출물은 50 ~ 100% 에탄올 추출물인 것을 특징으로 하는 주름 생성 억제 및 주름 개선용 식품 조성물.
The method of claim 6, wherein
Wherein the white tea extract is a 50-100% ethanol extract.
상기 백차(White tea) 추출물은 분말제, 액제, 정제, 연질캅셀제, 과립제, 티백차, 인스턴트 차 또는 드링크제로 제형화된 것을 특징으로 하는 주름 생성 억제 및 주름 개선용 식품 조성물.8. The method according to claim 6 or 7,
Wherein the white tea extract is formulated as a powder, a liquid, a tablet, a soft capsule, a granule, a tea bag, an instant tea or a drink.
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| KR1020120136469A KR101474998B1 (en) | 2012-11-28 | 2012-11-28 | Composition comprising extracts of white tea for the care of skin wrinkle |
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| KR20230092511A (en) | 2021-12-17 | 2023-06-26 | 한국콜마주식회사 | Perfume composition for expressing the fragrance of white tea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106726898A (en) * | 2016-12-07 | 2017-05-31 | 杭州银茗生物技术有限公司 | A kind of Ramulus et Folium Mussaendae Pubescentis extract and preparation method and application |
| JP6619530B2 (en) * | 2018-02-22 | 2019-12-11 | 株式会社ファンケル | Composition for inhibiting matrix metalloprotease production |
| CN115006317A (en) * | 2022-07-27 | 2022-09-06 | 水羊化妆品制造有限公司 | Method for enriching fuding white tea flavone by using natural eutectic solvent and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009500394A (en) | 2005-07-08 | 2009-01-08 | ザ プロクター アンド ギャンブル カンパニー | Personal care composition and method for beautifying mammalian skin and hair |
| WO2012046229A2 (en) | 2010-10-04 | 2012-04-12 | Cupron Scientific Ltd | Cosmetic skin care compositions |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2725985B2 (en) * | 1993-10-21 | 1998-03-11 | ハウス食品株式会社 | Mixed tea beverage |
| JP3459856B2 (en) * | 1995-09-29 | 2003-10-27 | 株式会社資生堂 | External preparation for skin |
| CN1929851B (en) * | 2004-01-12 | 2012-06-27 | 综合植物科技有限责任公司 | Bioactive compositions from theacea plants and processes for their production and use |
| DE602005026662D1 (en) * | 2004-06-25 | 2011-04-14 | Ferrosan As | COMPOSITIONS SUITED TO THE TREATMENT OF SKIN AGING SYMPTOMS |
| JP4944372B2 (en) * | 2004-12-03 | 2012-05-30 | 株式会社ナリス化粧品 | Acrolein adduct formation inhibitor, and skin external preparation and health supplement containing the same |
| AU2008206087A1 (en) * | 2007-01-18 | 2008-07-24 | Mark A. Pinsky | Materials and methods for delivering antioxidants into the skin |
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- 2012-11-28 KR KR1020120136469A patent/KR101474998B1/en active IP Right Grant
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009500394A (en) | 2005-07-08 | 2009-01-08 | ザ プロクター アンド ギャンブル カンパニー | Personal care composition and method for beautifying mammalian skin and hair |
| WO2012046229A2 (en) | 2010-10-04 | 2012-04-12 | Cupron Scientific Ltd | Cosmetic skin care compositions |
Non-Patent Citations (1)
| Title |
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| Korean J. Microscopy 41(3), 197-204, 2011* |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230092511A (en) | 2021-12-17 | 2023-06-26 | 한국콜마주식회사 | Perfume composition for expressing the fragrance of white tea |
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| JP2014105210A (en) | 2014-06-09 |
| KR20140068679A (en) | 2014-06-09 |
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