KR101188682B1 - Composition for skin whitening comprising extracts of Veronica longiofolia L. - Google Patents

Abstract

The present invention relates to a composition for skin whitening containing Veronica longifolila L. extract as an active ingredient, more specifically, Veronica longifolia extract is extracted by using water, alcohol or a mixture thereof as a solvent. It inhibits the production of melanin produced cell line and shows a whitening effect. Since there is little cytotoxicity, it can be usefully used as a skin whitening composition, cosmetic composition or health food.

Description

Composition for skin whitening using veronica rongifolia extract {Composition for skin whitening comprising extracts of Veronica longiofolia L．}

The present invention relates to a composition for skin whitening containing Veronica longiofolia L. extract as an active ingredient, and more particularly, to the Veronica longiofolia extract extracted by using water, alcohol or a mixture thereof as a solvent. It relates to a skin whitening composition or a cosmetic composition containing as a component.

Melanin is a pigment present in the epidermal layer of the skin and plays a useful role in protecting the skin and subcutaneous tissues from harmful ultraviolet radiation. Prolonged exposure to ultraviolet light has the risk of dying off chromosomes, including skin cancer, corneal damage, or single cell organisms. That is, the reason why the color of the skin changes when exposed to sunlight for a long time is that the melanocytes produced in the melanocytes of the basal layer of the skin to the keratinocytes, which are epidermal cells, to protect the skin and subcutaneous tissues from ultraviolet rays. This is because it changes the color of the skin as it moves. Depending on the amount of melanin in the skin cells, people with more pigment will have brown or black skin, while those with less melanin will have white skin.

In melanin synthesis, tyrosinase enzymes in melanocytes in the basal layer of the skin play an important role. Tyrosinase is normally present in melanocytes in an inactive state, but becomes active when subjected to ultraviolet rays to generate tyrosine amino acids via dopa (DOPA) to produce dopaquinone, and autooxidation and enzymatic reaction from dopaquinone. Dopa chromium (DOPAchromne) through the production of the copolymer melanin is produced.

If a problem with melanin synthesis occurs, abnormally low amounts of skin lesions such as vitiligo are caused, and conversely, if melanin accumulates due to excessive synthesis or keratinization of melanin, pigmentation is deposited on the skin. Freckles form skin cancers. Therefore, the study of melanin today is not only for satisfying the beauty desire for clean and white skin but also for the prevention and treatment of skin cancer, the composition having a skin whitening effect, the substance that inhibits the enzymatic activity of tyrosinase and tyrosinase inhibitor This leads to the development of formulations for permeation.

In the development of a whitening agent for skin beauty, the characteristic of a composition having a conventional skin whitening effect is a method of reducing melanin by reducing the melanin pigment produced by targeting melanin, which is the main cause of coloring, and of tyrosinase for reducing melanin synthesis. Methods of inhibiting activity are known. Skin whitening ingredients used to reduce pigmentation of melanin include substances that inhibit tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, and vitamin-C (L-Ascorbic). acid) and their derivatives and various plant extracts have been used. However, the skin whitening ingredients are poor in stability in the prescription system, and are degraded or colored, or their use is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety issues. In addition, skin whitening agents using tocopherol, vitamins, and the like used to reduce the melanin pigment are known to have a slight decolorizing effect of the melanin pigment, and many studies have been conducted on inhibitors that inhibit the production of melanin pigment.

In addition, it has been found that a number of plant extracts and herbal extracts act on tyrosinase to inhibit melanin production. However, in terms of stability and discoloration, there are many problems in using them in effective concentrations over pharmaceuticals. The situation is not effective.

Veronica (Veronica) genus is widely distributed, but mainly distributed in central, western Europe and Turkey region, the main component of the genus Veronica is flavonoids, it is known to have the efficacy of antibacterial, anticancer, antiviral, antiallergic. Veronica longifolia L. is a native Mongolian plant, and the skin whitening effect of a composition comprising Veronica longifolia extract as an active ingredient has not been reported.

Therefore, the present inventors intend to develop a new effective substance that can overcome the disadvantages of the safety and whitening effect of the existing whitening substance, to construct a composition for skin whitening containing Mongolian native plant veronica rongifolia extract as an active ingredient. This revealed a new effect that was not found in the existing veronica rongifolia, and furthermore, by adding veronica rongifolia extract to melanocytes, it was confirmed that melanin production was suppressed and there was almost no cytotoxicity, thereby completing the present invention. .

An object of the present invention is to provide a composition for skin whitening, a cosmetic composition for skin whitening, and a health functional food for skin whitening containing Veronica longifolia L. extract as an active ingredient.

In order to achieve the above object, the present invention provides a composition for skin whitening containing Veronica longifolia L. extract as an active ingredient.

The present invention also provides a cosmetic composition for skin whitening containing Veronica Rongifolia extract as an active ingredient.

In addition, the present invention provides a health functional food for skin whitening containing Veronica Rongifolia extract as an active ingredient.

Veronica longifolia L. extract of the present invention is an extract that inhibits the production of melanin, has revealed a novel effect not revealed in the previous Veronica longifolia research, and the whitening effect is not inferior to the existing whitening material Since there is little cytotoxicity, it can be usefully used as a skin whitening composition, cosmetic composition or health food.

1 is a graph showing the melanin synthesis inhibitory effect of Veronica Rongifolia methanol extract.
Figure 2 is a graph showing the cytotoxicity of the Veronica Rongifolia methanol extract.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for skin whitening containing Veronica longifolia L. extract as an active ingredient.

Veronica longifolia extract according to the present invention may be prepared as follows.

Veronica rongifolia is washed thoroughly with water and dried in the shade. Dry veronica rongifolia is placed in an extraction vessel and extracted with water, alcohol or a mixture thereof at a suitable temperature for a period of time. The extract can be filtered and concentrated.

As the extraction solvent, it is preferable to use a solvent selected from water, C ₁ to C ₄ lower alcohols, or a mixed solvent thereof, and more preferably to use methanol.

The amount of the extraction solvent is 2 to 10 times, preferably 4 times the weight of the herbal medicine.

The extraction method may be an extraction method such as heat extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as the extraction method of the heat extraction.

The deposition temperature is 30 to 70 ℃, more preferably 40 to 60 ℃, most preferably maintained at about 50 ℃ is most effective in the prevention of rot, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

In the embodiment of the present invention, when B16F10 melanocytes were treated with Veronica longifolia extract prepared in <Example 1>, there was a whitening effect of inhibiting melanin production by about 40% than the untreated group, which was a negative control group, as a general whitening material. Levels of inhibiting known arbutin and melanin production were similar (see Figure 1 and Table 1). In addition, treatment with melanin at a high concentration of 200 μg / ml showed little cytotoxicity (see FIG. 2 and Table 2).

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to Veronica longifolia extract.

The composition of the present invention can be administered in a variety of oral and parenteral formulations during actual clinical administration, can be used as a method of applying to the skin during parenteral use, it is possible to select the preferred method of use according to the formulation.

The dosage of the composition of the present invention is preferably 0.0001 to 50 parts by weight, more preferably 0.001 to 50 parts by weight, based on the amount of Veronica Rongifolia extract, but is not limited thereto.

The present invention also provides a cosmetic composition for skin whitening containing Veronica Rongifolia extract as an active ingredient.

Veronica Rongifolia extract was found to be inhibited by about 40% melanin production when treated with melanocytes, compared to untreated. Therefore, it can be effectively used as a cosmetic composition for whitening that suppresses excessive melanin production.

In the composition of the present invention, the veronica rongifolia extract is preferably contained in an amount of 0.005 to 50% by weight based on the total weight of the composition, but may be used in the above or below the range according to the use purpose in the range of whitening effect and no toxicity. have.

The composition of the present invention may further contain one or more active ingredients exhibiting the same or similar function in addition to the Veronica Rongifolia extract.

Cosmetics made of a cosmetic composition containing the veronica rongifolia extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the veronica rongifolia extract of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to Veronica longifolia extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, Ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, any of those commonly used in lipid vesicles or cosmetics It may contain adjuvants commonly used in the cosmetic or dermatological field, such as other ingredients of. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

In addition, the present invention provides a health functional food for skin whitening containing Veronica Rongifolia extract as an active ingredient.

Veronica Rongifolia extract of the present invention inhibits the production of melanin and inhibits tyrosinase enzyme activity, thereby exhibiting a whitening effect, and can be usefully used as an active ingredient for whitening health foods since there is little cytotoxicity.

Veronica longifolia extract of the present invention may be provided as a food composition by mixing with a food-acceptable carrier.

When the veronica rongifolia extract of the present invention is used as a food or beverage additive, the veronica rongifolia extract may be added as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the veronica rongifolia extract can be suitably determined according to the purpose of use (prevention, health or therapeutic treatment).

In the case of long-term intake for health and hygiene or for health control, the veronica longifolia extract can be taken for a long time because there is no problem in terms of safety.

There is no particular limitation on the kind of the food. Examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gum, ice cream, various soups, drinks, tea, and drinks. Alcoholic beverages and vitamin complexes.

The liquid component added in addition to the Veronica longifolia extract when formulated into a beverage is not limited to the above, but may contain various flavors, natural carbohydrates, etc. as additional ingredients, as in conventional beverages. Examples of the natural carbohydrates described above include monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.) and polysaccharides (e.g. conventional sugars such as dextrins, cyclodextrins, etc.), and xylitol Sugar alcohols such as sorbitol and erythritol. The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention. As flavoring agents other than those described above, natural flavoring agents (taumarin, stevia extract (e.g., Rebaudioside A, glycyrzin, etc.)) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.) can be used. .

In another embodiment, the food composition of the present invention comprises various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic and natural flavors, colorants and enhancers (such as cheese, chocolate), pectic acid and salts thereof, organic acids , Protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. The food composition of the present invention may also contain pulp for the production of fruit and vegetable drinks. These components may be used singly or in combination, and the proportion of such additives is generally selected in the range of 0.001 to 50 parts by weight based on the total weight of the composition.

Hereinafter, the present invention will be described in detail with reference to Examples and Production Examples.

However, the following Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Preparation Examples.

Example 1 Preparation of Veronica Rongifolia Extract

<1-1> Veronica Rongifolia Methanol Extract Preparation

200 g of Veronica longiofoila L. collected from Mongolia was soaked in 1.5 L of 100% methanol, and the extract obtained by hot water for 72 hours in a 50 ° C water bath was cooled to room temperature, filtered through a filter paper, and the supernatant was taken. It was. The supernatant was concentrated by evaporation of methanol using nitrogen to give 9.7 g. Then, when used in <Experimental Example>, the extract obtained after concentration was dissolved in 10% aqueous methanol solution.

<1-2> Veronica Rongifolia Ethanol Extract

According to the method of Example <1-1>, 6.7 g of Veronica Lon polyfolia ethanol extract was prepared, and 100% ethanol was used instead of methanol.

<1-3> Veronica Rongifolia Water Extract

According to the method of Example <1-1> was prepared 5.1g Veronica Rongifolia water extract, where water was used instead of methanol.

Experimental Example 1 Measurement of Whitening Effect

<1-1> B16F10 melanin cell culture

B16F10 melanocytes were supplied from Suwon Forestry Research Institute. Melanocytes are cell lines that produce melanin and are generally used for whitening screening.

The medium for culturing the cells was prepared aseptically by preparing and sterilizing DMEM medium containing 10% FBS, 1% penicillin / streptomycin. Cell cultures were incubated in an incubator at 37 ° C., 5% CO ₂ . Melanocytes were cultured and passaged when the 100 mm Petri dishes were about 90% full.

<1-2> Measurement of changes in melanin production

In order to measure the whitening effect of Veronica Rongifolia extract, the change of melanin synthesis was measured by absorbance when B16F10 melanocytes were treated with extract. In addition, by treating the existing whitening effect of arbutin (Arbutin), the whitening effect was examined. After inoculating melanin cells in a 6-well plate and incubating for 2 days with the concentration of 200 μg / ml of the veronica longifolia extract or arbutin prepared in Example 1, trypsin-EDTA Cells were harvested. 1N NaOH containing 10% DMSO (dimethylsulfoxide) was added to the recovered cells and reacted at 90 ° C. for 1 hour to sufficiently dissolve melanin, and then absorbance was measured at a wavelength of 405 nm using an ELISA reader. Melanin production rate (%) was calculated using the following [Equation 1].

As a result, as shown in Figure 1 and Table 1, when the veronica rongifolia extracts and <RTI ID = 0.0> arbutin </ RTI> of <Example 1> were treated to melanocytes, the veronica rongipolya methanol extract was compared with the untreated group, which was a negative control group. At this time, it was confirmed that about 40% of melanin production was inhibited, which was similar to that of arbutin. Therefore, the whitening effect of the composition containing Veronica Rongifolia methanol extract as an active ingredient could be confirmed.

Name of sample Melanin production (%) Untreated 100% Arbutin 43.4% The extract of Example < 1-1 > 57.2% Extract of Example <1-2> 60.0% Extract of Example <1-3> 62.0%

<Experimental Example 2> Cytotoxicity test

Cytotoxicity was tested to determine whether the Veronica Rongifolia extract could be used as a cell whitening agent. Cells were seeded in 96-well plates and treated with 200 μg / ml veronica longifolia extract and arbutin, respectively, and the cells were incubated for 2 days in an incubator at 37 ° C., 5% CO ₂ . After incubation, the medium was removed, and 100 μl of MTT (3- (4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazoliu, bromide) solution was added and reacted at 30 ° C. for 4 hours. After the reaction was completed, the solution was removed, and 100 μl of DMSO was added to dissolve the stained cells, and the absorbance was measured at a wavelength of 540 nm using an ELISA reader. Cell viability was calculated using the following [Equation 2].

As a result, as shown in Figure 2 and Table 2, when compared to the Veronica Rongifolia extracts of <Example 1> and arbutin, and the non-treated group as a negative control, the cells despite the high concentration of 200 ㎍ / ㎖ The survival rate was 96.2%, similar to that of arbutin, and showed little cytotoxicity.

Name of sample Cell survival rate (%) Untreated 100% Arbutin 96.3% The extract of Example < 1-1 > 96.7% Extract of Example <1-2> 96.3% Extract of Example <1-3> 96%

The preparation examples for the composition of the present invention are illustrated below.

Preparation Example 1 Preparation of Cosmetics

A cosmetic for skin whitening containing Veronica Rongifolia extract of the present invention as an active ingredient was prepared.

<1-1> flexible lotion

A flexible lotion containing veronica rongifolia extract was prepared as shown in Table 3 below.

Raw material Content (% by weight) Veronica Rongifolia Methanol Extract 10.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<2-2> nutrition cream

Nutritional cream containing Veronica Rongifolia extract was prepared as shown in Table 4 below.

Raw material Content (% by weight) Veronica Rongifolia Methanol Extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

Production Example 2 Preparation of Food

Foods containing the veronica rongifolia extract of the present invention were prepared as follows.

<2-1> Preparation of sweets and snacks

0.5-5.0 parts by weight of Veronica Rongifolia ethanol extract of the present invention was added to flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

<2-2> Production of Dairy Products

5 to 10 parts by weight of veronica rongifolia water extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<2-3> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The veronica rongifolia water extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a spray and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The dry powders of the grains, seeds and Veronica longifolia water extracts prepared above were prepared in the following ratio.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Dry powder (3 parts by weight) of Veronica longifolia water extract,

Ganoderma lucidum (0.5 parts by weight), and

(0.5 parts by weight)

Preparation Example 3 Preparation of Pharmaceutical Formulation

<3-1> Preparation of Powder

2 g of methanol extract of Example <1-1> of the present invention

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<3-2> Preparation of Tablet

100 mg of methanol extract of Example <1-1> of the present invention

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

<3-3> Preparation of Capsule

100 mg of water extract of Example <1-3> of the present invention

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

<3-4> Preparation of the ring

1 g of the water extract of Example <1-3> of the present invention

Lactose 1.5 g

1 g of glycerin

Xylitol 0.5 g

After mixing the above components, it was prepared to be 4g per ring in a conventional manner.

<3-5> Preparation of Granules

150 mg of water extract of Example <1-3> of the present invention

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100mg of 30% ethanol was added and dried at 60 ° C. to form granules, and then filled into fabric.

Claims (5)

A cosmetic composition for skin whitening containing Veronica longifolia L. extract as an active ingredient.
The cosmetic composition for skin whitening according to claim 1, wherein the extract is extracted with water, C ₁ to C ₄ lower alcohols, or a mixture thereof.
The cosmetic composition for skin whitening according to claim 2, wherein the lower alcohol is ethanol or methanol. delete delete

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