KR102471009B1 - Cosmetic composition containing Albiggia kalkora extract for skin whitening and improving wrinkles - Google Patents

Abstract

The present invention relates to a composition containing an extract of Prince Philippine, in which the extract of Philippine, especially the stem and branch extract, suppresses the activity of tyrosinase and collagenase and has excellent radical scavenging activity, thereby improving the skin It can be easily used as a multi-functional cosmetic composition that improves the condition.

Description

Cosmetic composition containing Albiggia kalkora extract for skin whitening and improving wrinkles}

The present invention relates to a cosmetic composition for skin whitening or anti-wrinkle containing an extract of japonica japonica.

Skin is a place covering the surface of the body and is divided into epidermis and dermis. The epidermis serves to protect internal organs, and skin cell proliferation is performed by the activity of the fibroblasts in the dermis. In addition, the dermis of the skin is composed of fibers of extracellular matrix proteins such as collagen, elastin, and fibrillin woven into a net shape (Braverman, J. Invest. Dermatol., 78, 434-443, 1982) overall, it plays a role in maintaining the elasticity of the entire skin. As the activity of fibroblasts, which are the basis of skin, declines, the number of cells decreases, and when collagen and elastin decrease, wrinkles of the skin increase. It is known that the protein biosynthetic activity of fibroblasts decreases with age. Cosmetics for improving skin wrinkles have been continuously developed for a long time. Especially, the case of skin wrinkles in women is a very sensitive matter. For the occurrence of skin wrinkles that are thought to be caused by external factors, UV blockers or moisturizers etc. are pouring out with various products.

Retinoic acid, retinol, and vitamin C are representative examples of materials that promote collagen synthesis as a skin wrinkle improvement material. However, each substance has a disadvantage. In the case of retinoic acid, it is highly irritating to the skin, and retinol itself is low in stability, so it is easily oxidized or causes skin trouble. In addition, vitamin C is also easily deteriorated by air, temperature, etc., and it is known that there are many problems with stability.

On the other hand, hyperpigmentation of the skin is caused by various factors such as hormone abnormalities in the body after an inflammatory reaction of the skin, genetic diseases, and ultraviolet irradiation, and the main factor is due to abnormal melanin synthesis and distribution. Melanin is produced by converting tyrosine into dopaquinone by the action of tyrosinase in melanocytes, and then converting the dopaquinone into melanin by the action of enzymes and spontaneous oxidation reactions.

Methods for inhibiting melanin production include the following methods. The first method is to remove the main cause of melanin production by blocking ultraviolet rays, and a whitening reaction can be induced by preparing a cosmetic containing a light scattering agent or a light blocking agent. . The second method is a method of inhibiting melanin production by inhibiting the synthesis of core carbohydrates required for tyrosinase to be active, and a compound such as glucosamine may be used. A third method is a method of interfering with cell division of the melanocytes by using a substance having specific toxicity to melanocytes, and a compound such as hydroquinone may be used. A fourth method is a method of interfering with the function of tyrosinase, an enzyme involved in melanin production, and a compound such as kojic acid or arbutin may be used. As another method, there is also a method of reducing the produced melanin. When using compounds such as hydroquinone, kojic acid, or arbutin in the above method, compounds derived from natural products may be used, but synthetic compounds may also be used. Representatively used synthetic compounds include 4-n-butylresorcinol, quercetin, kaempferol, etc., in addition to hydroquinone and kojic acid or arbutin mentioned above. Although the synthetic compounds have good whitening effects, their use has been limited due to product safety problems caused by skin irritation. In particular, synthetic 4-n-butylresorcinol is easily browned due to poor stability during product production, and skin irritation may occur when used in high concentrations. Therefore, in order to solve these disadvantages, a method of liposomizing 4-n-butylresorcinol using gamma-linolenic acid has been disclosed (Korean Registered Patent No. 10-0751883), but it was impossible to completely eliminate irritation. .

Antioxidant function is to suppress or slow down the oxidation reaction that occurs in the body. The oxidation reaction that occurs in the body induces aging and cell mutation to generate cancer cells. Therefore, the public is also interested in antioxidant action as a preventive measure to suppress such negative reactions in the body. In the body, mitochondria in cells whose main function is to produce ATP, which corresponds to energy along with cellular respiration, generate free radicals, which are by-products of respiration, which are unstable and rapidly and indiscriminately different from other substances such as lipids, nucleic acids, and proteins. react negatively Therefore, if the generation of these free radicals can be prevented, it can be seen as preventing oxidation in the body, and indirectly, aging and cancer can be prevented, which is of great interest. This antioxidant effect is unreasonable to experiment with free radicals generated in the human body, so DPPH (1,1-diphenyl-2-picylhydrazyl) radical is used as a biomarker. DPPH radical scavenging activity analysis is one of the methods for measuring the antioxidant activity of a sample. It is reduced by receiving hydrogen or electrons by phenolic compounds, aromatic amines, and ascorbic acid, and uses the principle that the purple color of DPPH is decolored. It is a widely used method because it is relatively simple and can measure antioxidant activity in a short time and has a high correlation with the antioxidant activity of plants.

Albiggia kalkora PRAIN is a deciduous tree belonging to the leguminous family, native to Korea and partially distributed in China. Compared to the silk tree, the leaves are larger and there are more stamens, and the flowers are more white. Although the bark of the japonica tree has been used as a herbal medicine for a long time, there is little academic research on its various uses.

Accordingly, the inventors of the present invention completed the present invention by confirming that the extract has excellent skin whitening, anti-wrinkle, or antioxidant effects while studying various physiological activities of the extract of japonica japonica.

Republic of Korea Patent Registration No. 10-1225103 (Title of Invention: Insecticide use of a composition containing silkworm tree extract, Applicant: Korea Research Institute of Chemical Technology and one other person, Registration date: January 16, 2013) Republic of Korea Patent Registration No. 10-0443588 (Title of invention: Anti-aging cosmetic composition containing Moutan bark extract and silk tree extract, Applicant: Nadri Cosmetics Co., Ltd. and one other person, Registration date: July 28, 2004) Republic of Korea Patent Registration No. 10-1081059 (Title of Invention: Flower Mixture Extract with Antioxidant and Whitening Activity, Extraction Method and Cosmetic Composition Containing the Flower Mixture Extract, Applicant: Kolmar Korea Co., Ltd., Registration Date: November 01, 2011 Work)

An object of the present invention is to provide a cosmetic composition for skin whitening or anti-wrinkle containing an extract of japonica japonica.

The present invention relates to a cosmetic composition for skin whitening or wrinkle improvement containing an extract of Albiggia kalkora PRAIN.

The extract has an activity of inhibiting tyrosinase or collagenase.

In addition, the extract is characterized in that it has antioxidant activity.

The extract is characterized in that it is extracted using water, C1-C4 alcohol or a mixed solution thereof as a solvent. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.

The cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, foundation, essence, nutrient essence, pack, soap , It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

In addition, the present invention may relate to a health functional food for skin whitening or wrinkle improvement containing an extract of japonica japonica. The health functional food is characterized in that it has an antioxidant effect.

Hereinafter, the present invention will be described in detail.

The extract of japonica may be obtained by extracting leaves, stems, stems, bark, roots, flowers, fruits, or mixtures thereof of the collected cyperaceae plants using an extraction solvent. Preferably, a mixture of branches and stems in a weight ratio of 1:0.5 to 1:2 may be used.

The princely japonica extract can be prepared using water, C1-C4 alcohol, or a mixed solution thereof as a solvent. As the water, any water without impurities such as distilled water and ionized water (DI water) may be used. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. At this time, it is preferable that the extract of royal japonica is a C1-C4 alcohol extract or an aqueous solution extract of C1-C4 alcohol rather than a water extract, and an aqueous solution extract of C1-C4 alcohol has the best skin whitening, wrinkle improvement or antioxidant activity. Suitable as an aqueous solution of C1-C4 alcohol is a 50 to 90% (v/v) aqueous ethanol solution, and most suitable is a 60 to 70% (v/v) aqueous ethanol solution.

In the production of the princely chinensis, extraction can be performed using a solvent weighing 5 to 40 times, preferably 10 to 20 times the dry weight of the princely chinensis. If the solvent is too less than the above range, when preparing the extract by applying heat, extraction may not be performed well due to evaporation of the solvent, and if the solvent is too large, there is a problem in that the extraction time is too long.

In addition, the extraction process may be carried out while stirring at 10 to 40 ° C. for 24 to 48 hours, and extraction may be repeated 1 to 4 times to obtain a liquid extract.

The liquid extract may be filtered out using sterile gauze or filter paper. The filtrate through this filtration process can be concentrated under reduced pressure at 10 to 40 ° C using a rotary vacuum concentrator, and an extract can be obtained after removing the remaining solvent by lyophilization.

The royal japonica extract may be included in the cosmetic composition in an amount of 0.01 to 20% by weight. If less than 0.01% by weight of the royal apricot tree extract is included in the cosmetic, skin whitening, wrinkle improvement or antioxidant effects may be insignificant, and if it exceeds 20% by weight, formulation may be difficult. In addition, if it exceeds 10% by weight, the degree of improvement in skin whitening, wrinkle improvement, or antioxidant functionality may be insignificant, so it may be preferable that 0.01 to 10% by weight of the Philippine extract is included in the cosmetic composition. At this time, the weight of the princely tree extract is based on the dried powdered form of the princely tree extract.

In addition, the present invention may provide a cosmetic formulation containing the cosmetic composition as a raw material or an active ingredient. The formulations are external skin ointment, essence, whitening cream, lotion, emulsion, pack, general lotion, skin milk, skin, cream, serum, beauty soap, softening lotion, medicated lotion, body cleanser, cleansing oil, cleansing cream, cleansing tissue And cleansing water can be provided.

In the cosmetic formulation of the present invention, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, silica, aluminum hydroxide, Calcium silicate, polyamide powder, chlorofluorohydrocarbon, propane/butane or dimethyl ether, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan, ethoxylated isostearyl alcohols, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonites, Agar or tracanth, aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyl taurates, sarcosinates, fatty acid amide ether sulfates, alkylamidobetaines, fatty acid glycerides , fatty acid diethanolamide, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, the present invention provides a health functional food for skin whitening, anti-wrinkle or anti-oxidation containing the extract of the genus japonica.

The princely japonica extract may be added to the health functional food of the present invention in an amount of 0.01 to 100% by weight. The health functional food of the present invention includes forms such as tablets, capsules, pills or liquids, and foods to which the extract of the present invention can be added include, for example, various drinks, meat, sausages, bread, candy, Snacks, noodles, ice cream, dairy products, soups, ionic beverages, soft drinks, alcoholic beverages, chewing gum, tea, and vitamin complexes.

The present invention relates to a composition containing an extract of Prince Philippine, in which the extract of Philippine, especially the stem and branch extract, suppresses the activity of tyrosinase and collagenase and has excellent radical scavenging activity, thereby improving the skin It can be easily used as a multi-functional cosmetic composition that improves the condition.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the invention to those skilled in the art, so that the disclosure herein will be thorough and complete.

Preparation Examples 1 to 4. Preparation of extracts of japonica or japonica

In order to prepare the extract of the japonica japonica of the present invention, a mixture of branches and stems of the japonica japonica in equal amounts was washed with tertiary distilled water and finely pulverized using peas. 70% (v/v) ethanol aqueous solution was added 10 times by weight to the mixture of the branches and stems of the pulverized princeps tree, and extraction was performed while stirring at 25° C. for 24 hours. Thereafter, the filtrate was first filtered using sterile gauze, and then the filtrate was filtered again using filter paper (Whatman, No. 2: Whatman Ltd., Maidstone, Kent, UK), and the pulverized sample was filtered using a rotary vacuum concentrator. After concentration under reduced pressure, the remaining solvent was removed using a lyophilizer to obtain an extract of japonica japonica. In addition, as shown in Table 1 below, each extract was prepared for silkworm tree using a mixture of branches and stems, flowers, and leaves.

Condition sample Preparation Example 1 Princes Tree Branches and Stems Preparation Example 2 silk tree branches and stems Preparation Example 3 silk tree flowers Production Example 4 silk tree leaves

Example 1. Confirmation of collagenase inhibitory activity

The extracts prepared in Preparation Examples 1 to 5 were dissolved in dimethylsulfoxide (DMSO) to a final concentration of 150 μg/ml to confirm the inhibitory activity of collagenase, which is shown in Table 2. Collagenase inhibitory activity was measured according to the method of Wunsch and Heindrich. For the reaction sphere, 0.3 mg/mL of 4-phenyl azobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Sigma Aldrich CO., USA) was prepared by adding 4 mM CaCl ₂ to 0.1 M tris-HCl buffer (pH 7.5). 75 μL of 0.2 mg/mL collagenase (Sigma Aldrich CO., USA) was added to a mixture of 125 μL of dissolved substrate solution and 50 μL of sample solution, and allowed to stand at room temperature for 20 minutes. After stopping the reaction by adding 250 μL of 6% citric acid, 1.5 mL of ethyl acetate was added to measure the absorbance at 320 nm with a UV/VIS spectrophotometer (Optizen 2120 UV, Mecasys, Daejeon, Korea). Collagenase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added group and non-added group.

Collagenase activity inhibition rate (%) = (B / A) × 100

A: Absorbance value of the non-added group

B: absorbance value after extract treatment

division Absorbance relative to control
(Relative collagenase activity, Abs) Control (DMSO) 1.00 Preparation Example 1 0.15±0.08 Preparation Example 2 1.22±0.07 Preparation Example 3 1.01±0.09 Production Example 4 0.83±0.03

As a result, as shown in Table 2, it can be confirmed that the inhibitory effect of collagenase is the best in the japonica extract treatment group.

Example 2. Confirmation of tyrosinase inhibitory activity

To measure Tyrosinase inhibitory activity, mushroom tyrosinase, which is widely used in general, was used. A solution of 1 mM L-tyrosine and 0.5 M phosphate buffer (pH 6.5) is prepared in a 96-well microplate (SPL Life Sciences Co., Ltd., Pocheon, Korea). Extract samples to be measured were reacted with 170 μl of the prepared solution and 20 μl of tyrosinase (300 U) at 25° C. for 30 minutes. After the reaction, it was measured at 450 nm using a microplate reader (Berthold, Bad Wildbad, Germany). 10 μl of DMSO was added and used as a control. Blank was measured by adding 20 μl of 0.1 M phosphate buffer (pH 6.5) instead of tyrosinase.

The enzyme activity inhibition rate (%) was calculated by the following formula.

Tyrosinase activity inhibition rate (%) = {(A-B)/A} × 100

A: O.D at 450 nm without sample,

B: O.D at 450 nm with sample

division Absorbance relative to control
(Relative tyrosinase activity, Abs) Control (DMSO) 1.00 Preparation Example 1 0.60±0.02 Preparation Example 2 2.02±0.03 Preparation Example 3 1.52±0.01 Production Example 4 1.23±0.02

As a result of the confirmation, as shown in Table 3, it can be confirmed that the inhibitory effect of tyrosinase is the best in the A. japonica extract treatment group.

Example 3. DPPH radical scavenging ability test

Sample solutions were prepared by dissolving the branch and leaf extracts of Almond tree prepared in Preparation Example 1 and the extracts for each part of Almond tree, respectively. 10 μl of each extract sample was dispensed into each well of a 96-well plate to have a final concentration of 10, 50, and 100 μg / ml, and 190 μl of 150 μM DPPH (1,1-diphenyl-2-picrylhydrazyl) ethanol solution was added and reacted. Then, after covering the lid, it was reacted at 25 ° C. for 30 minutes. After the reaction was completed, the absorbance value at 517 nm was measured for the color change of DPPH using a spectrometer (Microplate reader). The above process was repeated three times and the averaged value was used, and the results are shown in Table 4 below.

division at 10 μg/ml
DPPH radical scavenging activity (%) at 50 μg/ml
DPPH radical scavenging activity (%) at 100 μg/mL
DPPH radical scavenging activity (%) Preparation Example 1 55.7±0.7 82.8±0.2 98.3±2.6 Preparation Example 2 6.6±0.7 16.6±0.7 32.1±1.3 Preparation Example 3 5.4±0.9 21.4±0.9 36.2±3.9 Production Example 4 8.4±1.1 20.4±1.1 39.2±2.3

Referring to Table 4, the extract of Preparation Example 1 removes almost 50% or more of DPPH radicals from 10 μg/ml, but each extract of silkworm tree has less than 40% DPPH radicals even when treated with 100 μg/ml. , it can be seen that there is a significant difference between each plant in antioxidant capacity.

Example 4. Confirmation of ROS measurement values

HDF (human dermal fibroblast) cells were cultured and dispensed at 5×10 ³ cells/ 100 μl per well in a 96 well plate, cultured in an incubator for 24 hours, and then the culture medium was removed and treated with a sample or positive control (quercetin 10 μM). After that, it was cultured for 24 hours in an incubator. After removing all the culture medium in the well under light-shielding conditions, washing with PBS solution, dissolving 2-7-dicholordihydrofluorescein diacetate (DCF-DA) in DMSO to 10,000 μM, dispensing in small amounts and storing it frozen. After washing once more with PBS, 50 μl of 50 μM DCF-DA solution was added and incubated in an incubator for 30 minutes. ROS indicator H ₂ O ₂ was diluted to a concentration of 200 μM in PBS (containing 1% FBS), treated with 200 μl in each well, and then wrapped in foil and incubated in an incubator for 30 minutes. Fluorescence was measured at 485 nm and emission at 535 nm.

As a result of confirming the ROS scavenging ability generated by the 200 µM H ₂ O ₂ treatment, as shown in Table 5 below, the ROS scavenging ability of the P. japonica extract was found to be more than twice as high when treated with 100 µg/ml. At this time, the ROS concentration was measured based on 100% of the normal group not treated with H ₂ O ₂ .

As a result, as shown in Table 5 below, it can be confirmed that the ROS concentration, which was increased by more than twice as much as the normal group due to the H ₂ O ₂ treatment, was remarkably reduced by the treatment with the Almond tree extract. does not seem to indicate

sample treatment group ROS concentration comparison value (%) at 100 μg/ml extract H ₂ O ₂ (single treatment) 211.6±15 H ₂ O ₂ + Preparation Example 1 42.1±1.9 H ₂ O ₂ + Preparation Example 2 150.1±2.5 H ₂ O ₂ + Preparation Example 3 146.2±4.9 H ₂ O ₂ + Preparation Example 4 159.2±2.8

<Formulation Example 1. Cosmetics Manufacturing>

Formulation Example 1-1. manufacture of skins

5.0% by weight of the extract of japonica extract of Preparation Example 1 of the present invention, 5.2% by weight of propylene glycol, 1.5% by weight of oleyl alcohol, 3.2% by weight of ethanol, 3.2% by weight of polysorbate 20, 2.0% by weight of benzophenone-9, caramel A skin was prepared using a conventional method with the contents of 1.0% by weight of boxyl vinyl polymer, 3.5% by weight of glycerin, a trace amount of fragrance, a trace amount of preservative, and the remaining amount of purified water.

Formulation Example 1-2. manufacture of lotion

5.0% by weight of the extract of japonica extract of Preparation Example 1 of the present invention, 1.0% by weight of cetostearyl alcohol, 0.8% by weight of glyceryl monostearate, 0.3% by weight of sorbitan monostearate, 1.0% by weight of polysorbate 60, minerals 5.0% by weight of oil, 3.0% by weight of cyclomethicone, 0.5% by weight of dimethicone, 0.1% by weight of allantoin, 5.0% by weight of glycerin, 2% by weight of alcohol, 3.0% by weight of propylene glycol, a small amount of flavor, a small amount of preservative, and the remaining amount of purified water A lotion was prepared using a conventional method.

Formulation Example 1-3. Preparation of Essence

5.0% by weight of the extract of Mango japonica of Preparation Example 1 of the present invention, 4.0% by weight of propylene glycol, 3.0% by weight of glycerin, 0.5% by weight of allantoin, 0.01% by weight of EDTA-2Na, 5.0% by weight of ethanol, 1.5% by weight of triethanolamine, squalane 2.0% by weight, 2.5% by weight of beeswax, 60% by weight of polysorbate, 1.0% by weight of carboxylvinyl polymer, 2.5% by weight of sorbitan sesquioleate, a small amount of flavor, a small amount of preservative, and the remaining amount of purified water using a conventional method Essence was prepared.

Formulation Example 1-4. preparation of cream

10.0% of the extract of japonica extract of Preparation Example 1 of the present invention, 0.7% of polyoxyethylene sorbitan monostearate, 0.5% of sorbitan sesquioleate, 0.6% of cetyl alcohol, 0.75% of stearic acid, 0.6% of glyceryl monostearate, Cream was prepared using a conventional method with the contents of 15.0% liquid paraffin, 10.0% carboxyvinyl polymer, 0.2% triethanolamine, trace amounts of flavor, trace amounts of preservatives, and the remaining amount of purified water.

<Formulation Example 2. Food Manufacturing>

Formulation Example 2-1. Preparation of seasoning for cooking

A health-promoting cooking seasoning was prepared by adding 1% by weight of the royal crown extract of Preparation Example 1 of the present invention to the cooking seasoning.

Formulation example 2-2. Manufacture of Flour Food

0.1% by weight of the royal japonica extract of Preparation Example 1 of the present invention was added to wheat flour, and bread, cakes, cookies, crackers, and noodles were prepared using the mixture to prepare health-enhancing foods.

Formulation Example 2-3. Preparation of soups and gravies

Health-promoting soup and broth were prepared by adding 0.1% by weight of the kingwort extract of Preparation Example 1 of the present invention to soup and broth.

Formulation Example 2-4. Manufacture of dairy products

0.1% by weight of the japonica extract of Production Example 1 of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 2-5. Preparation of vegetable juice

Vegetable juice for health promotion was prepared by adding 0.5 g of the japonica extract of Production Example 1 of the present invention to 1,000 ml of tomato juice or carrot juice.

Formulation Example 2-6. manufacture of fruit juice

Fruit juice for health promotion was prepared by adding 0.1 g of the japonica extract of Production Example 1 of the present invention to 1,000 ml of apple juice or grape juice.

Claims (5)

A cosmetic composition for skin whitening or wrinkle improvement, characterized in that it contains an extract of Albiggia kalkora PRAIN, which has collagenase inhibitory activity. According to claim 1,
The extract is a cosmetic composition for skin whitening or wrinkle improvement, characterized in that there is an activity to inhibit tyrosinase. delete According to claim 1,
The extract is a cosmetic composition for skin whitening or wrinkle improvement, characterized in that it has antioxidant activity. A health functional food for skin whitening or wrinkle improvement, characterized in that it contains an extract of Albiggia kalkora PRAIN, which has an inhibitory activity on collagenase.