KR101081059B1 - Flowers Extract Having Anti-oxidation And Whitening Effects And Extraction Method Thereof And Cosmetics Comprising Flowers Extract - Google Patents

Abstract

The present invention relates to a flower mixture extract having antioxidant and whitening activity, a method of extracting the same, and a cosmetic composition containing the flower mixture extract, and more particularly, the flower mixture extract having antioxidant and whitening activity is an ascites flower ( Adonis amurensis). , Akebia quinata, Albizia julibrissin , Althaea rosea , Angelica acutiloba, Angelica anomala , Aquilegia buergeriana , Artemisa princeps , Aster ciliosus , Aster scaber , Rape flowers ( Brassica campestris ), Azalea ( Broussonetia kazinok ), Aster ( Callistephus chinensis ), Camellia ( Camellia japonica ), Camellia ( Camellia sinensis ), Jacaranda ( Camellia sinensis ) Campsis grandiflora ), Calendula ( Caragana sinica ), Safflower ( Carthamus tinctorius ), Cassia obtusifolia , Cockscomb ( Celosia cristata ), Cercis chinensis , Chinese quince ( Cha enomeles sinensis ), Chrysanthemum indicum , Chrysanthemum zawadskii , Sesame ( Clematis turniflora ), Commelina communis , Cornus kousa , Cornus officinalis , Cornucopia flower (Corydalis incisa), Corydalis flower (Corydalis turtschaninovii), hobakkkot (Cucurbita moschate), nankkot (Dendrobium nobile), Dianthus (Dianthus chinensis), enjoy flowers (Elsholtzia splendens), bone spine herbs flowers (Eupatorium lindleyanum), forsythia flowers ( Forsythia koreana , Fritillaria ussuriensis , Gardenia jasminoides , Ginkgo biloba , Helianthus annuus , Helianthus tuberosus , Hemerocallis fulva , Bouillon ( Hibiscus mutabilis ), Rose of Sharon ( Hibisicus syriacus ), Daylily ( Hosta plantaginae ), Hydrangea ( Hydrangea macrophylla ), Impatiens ( Impatiens balsamina ), Taraxacum ( Iris pallasii ), Iris pseudaco rus ), Yellow Plum ( Kerria japonica ), Lagerstroemia indica , Motherwort ( Leonurus sibiricus ), Lespedeza bicolor , Lilium lancifolium , Lindera obtusiloba , Linden obtusiloba Liriope muscari), honeysuckle (Lonicera japonica), the fall lobster Whatsoever flower (Lycoris radiata), magnolia (magnolia kobus), jamokryeon (magnolia liliflora), pear blossoms (Malus sieboldii), daffodils (narcissus tazetta), lotus (Nelumbo nucifera), Evening Primrose (Oenothera biennis), peony flower (Paeonia lactiflora), peonies (Paeonia suffruticosa), one yanggwibikkot (Papaver rhoeas), matari flowers (Patrinia scabiosifolia), paulownia flowers (paulownia coreana), gomanyi flower (Persicaria thunbergii), coltsfoot flowers (Petasites japonicus , Morning Glory ( Pharbitis nil ), Lilium ( Phytolacca esculenta ), Bellflower ( Platycodon grandiflorus ), Polygonatum odoratum , Poncirus trifoliata , Apricot ( Prunus armeniaca ), Plum ( Prunus mume ) Degree (Prunus persica), cherry (Prunus serrulata), Cherry blossom (Prunus tomentosa), chilkkot (Pueraria lobata), Jindal rekkot (Rhododendron mucronulatum), acacia flowers (Robinia pseudacacia), jjilrekkot (Rosa multiflora), sweetbrier (Rosa rugosa), Sasa borealis , Sophora japonica , Spiraea prunifolia , Styrax japonica , Syringa vulgaris , Taraxacum platycarpum, Taraxacum platycarpum, Tulipa edulis Two or more flowers of violet ( Viola mandschurica ), weedel ( Weigela subsessilis ), wisteria ( Wisteria sinensis ), zinnia ( Zanthoxylum schinifolium ), corn flower ( Zea mays ), yellow flower ( Zingiber mioga ) and Zinnia elegans ( Zinnia elegans ) A flower mixture extract having antioxidant and whitening activity extracted by adding water or an organic solvent or a mixture thereof to the mixture, and a method for extracting the same and a cosmetic composition containing the flower mixture extract to be.

The present invention as described above, by removing the oxides in the skin protects the skin, prevents aging and inhibits the production of melanin in the skin cells to help whitening, the cosmetic composition prepared by adding the extract also Antioxidant and whitening effect is remarkable.

Flower mixture extract, antioxidant, whitening, cosmetic composition, organic solvent, water, extraction, immersion

Description

Flower Extract Having Anti-oxidation And Whitening Effects And Extraction Method Thereof And Cosmetics Comprising Flowers Extract}

The present invention relates to a flower mixture extract having antioxidant and whitening activity, a method of extracting the same, and a cosmetic composition containing the flower mixture extract, and more particularly, the flower mixture extract having antioxidant and whitening activity is mixed with various kinds of flowers. It is a flower mixed extract having antioxidant and whitening activity extracted from the extract, wherein the cosmetic composition is an antioxidant that is a cosmetic composition that exhibits excellent antioxidant and whitening activity by adding the flower mixture extract to an ordinary cosmetic base in an amount of 0.01 to 10% by weight. And a flower mixed extract having a whitening activity, a method for extracting the same, and a cosmetic composition containing the flower mixed extract.

In general, for plants, flowers give priority to species preservation. However, these flowers contain various pigment components of anthocyanin-based, carotenoid-based, and flavonoid-based by each color, and these components exhibit various functions as their kinds.

Anthocyanin, which is typically contained in the flowers of plants, is called flower cleaning and means Anthocyan and its aglycon, anthocyanidin. Basically, all of them exist as glycosides in the cell, and they show red, blue, and purple color. When hydrolyzed into acid, it is a water-soluble pigment divided into red part (Anthocyanidin) and sugar part, which promotes cell aging and causes various adult diseases. Improves liver function by activating antioxidant function to remove oxygen, antimutagenic action to inhibit carcinogen, inhibiting metabolic factors causing cardiovascular diseases such as hypertension, arteriosclerosis, myocardial infarction, and eliminating cholesterol in body and liver As a result, various effects such as prevention and treatment of fatty liver, cirrhosis and alcoholic liver disease, improvement of brain metabolism function and prevention of dementia by promoting blood circulation have been revealed.

Flavonoids are also called Vit P or Vit C ₂ (Vit C synergists) and are yellowish plant pigments. Flavonoid (Flavonoid) is a plant pigment with Flavone as its basic structure, meaning Anthoxanthin. It is an acid-soluble water-soluble pigment that shows many effects such as antibacterial, anticancer, antiviral, antiallergic, and antiallergic effects.

In addition, carotenoids, which are yellow, orange, and pink insoluble pigments, are largely divided into carotenes (containing no oxygen in the molecule) and xanthopylls (containing oxygen). Β-carotene, Lycopene, and the latter are Lutein, Zeaxanthin, and Violaxanthin. These carotenoid pigments also have a variety of functions, such as antioxidant, immune enhancing, anticancer, vascular disease improvement, aging inhibitory action.

In addition, there are a variety of pigments in the flowers of plants, such as chlorophyll and betacyanin (nitrogen-containing anthocyanin), Cytochrome (pigment protein), Quinone-based, and Curmarin-based pigments.

However, these flowers are used as foods, such as tea, in addition to the kind used as some medicine, the use range is extremely limited.

In order to solve such a problem, a technology related to a composition (Korean Patent Registration No. 864,915) containing a mixed plant extract of plum and pear blossoms having antioxidant activity as an active ingredient is disclosed. However, the composition containing the mixed plant extracts of plum and Ewha having the antioxidant activity as an active ingredient is limited in the type of flowers used, maximizing the ingredients useful for the skin based on various ingredients and efficacy of several other flowers Problems remain difficult to develop.

Accordingly, an object of the present invention is to prepare a flower mixture extract having antioxidant and whitening activity extracted by adding an extraction solvent to various types of flower mixture extract to the problem of limitation as a cosmetic raw material by using a conventional limited flower mixture extract, The anti-oxidative and superoxide dismutase-like activities of the extracts demonstrated the antioxidant and whitening effects of inhibition of tyrosinase activity and inhibition of melanin biosynthesis in mouse-derived melanoma cells. It is an object of the present invention to provide a cosmetic composition containing an antioxidant and a whitening activity, and a method of extracting the same and a flower mixture extract, which have been developed to solve the problem by forming a cosmetic composition having no limitation as a cosmetic raw material.

In order to solve the object as described above, the present invention is a flower mixture extract,

Adonis amurensis, Akebia quinata, Albizia julibrissin , Althaea rosea , Angelica acutiloba, Angelica anomala , Aquilegia buergeriana , ssukkkot (Artemisa princeps), gaessukbujaengyi flowers (Aster ciliosus), Aster scaber flowers (Aster scaber), rape flower (Brassica campestris), mulberry flowers (Broussonetia kazinok), gwakkot (Callistephus chinensis), Camellia (Camellia japonica), tea flowers (Camellia sinensis , Campsis grandiflora , Caracuna sinica , Safflower ( Carthamus tinctorius ), Cassia obtusifolia , Celosia cristata , Cercis chinensis , Chaenomeles sinensis , gamguk (Chrysanthemum indicum), gujeolcho flowers (Chrysanthemum zawadskii), true euahri flowers (Clematis turniflora), dayflower flowers (Commelina communis), wild strawberry tree flower (Cornus kousa), Cornus officinalis flower (Cornus officinalis), often goebul pockets flowers (Co rydalis incisa), Corydalis flower (Corydalis turtschaninovii), hobakkkot (Cucurbita moschate), nankkot (Dendrobium nobile), Dianthus (Dianthus chinensis), enjoy flowers (Elsholtzia splendens), Bone Spine herbs flowers (Eupatorium lindleyanum), forsythia flowers (Forsythia koreana , Fritillaria ussuriensis , Gardenia jasminoides , Ginkgo biloba , Helianthus annuus , Helianthus tuberosus , Hemerocallis fulva , Hibiscus mutabilis ), Hibisicus syriacus , Daylily ( Hosta plantaginae ), Hydrangea ( Hydrangea macrophylla ), Impatiens balsamina , Iris pallasii , Iris pseudacorus , Yellow plum ( Kerria japonica ) tree flower (Lagerstroemia indica), flowers motherwort (Leonurus sibiricus), Hagi tree flowers (Lespedeza bicolor), true lilies (Lilium lancifolium), ginger tree flower (Lindera obtusiloba), maekmundong flowers (Liriope muscari), honeysuckle (Lon icera japonica ), lobster ( Lycoris radiata ), magnolia ( Magnolia kobus ), magnolia ( Magnolia liliflora ), pear ( Malus sieboldii ), narcissus ( Narcissus tazetta ), lotus ( Nelumbo nucifera ), evening primrose ( Oenothera biennis ), peony flower (Paeonia lactiflora), peonies (Paeonia suffruticosa), one yanggwibikkot (Papaver rhoeas), matari flowers (Patrinia scabiosifolia), paulownia flowers (paulownia coreana), gomanyi flower (Persicaria thunbergii), coltsfoot flowers (Petasites japonicus), morning glory (Pharbitis nil ), phytolacca esculenta , bellflower ( Platycodon grandiflorus ), oleander ( Polygonatum odoratum ), tangerine ( Poncirus trifoliata ), apricot ( Prunus armeniaca ), plum ( Prunus mume ), iris ( Prunus persica ), Prunus serrulata , Prunus tomentosa , Pueraria lobata , Rhododendron mucronulatum , Acacia ( Robinia pseudacacia ), Rosa multiflora , Rosa rugosa , Rose flower ( Sasa borealis) ), Sophora japonica , Spiraea prunifolia , Styrax japonica , Syringa vulgaris , Taraxacum platycarpum, Tulipa edulis , Viola mandschurica Water or organic in a mixture of two or more of the flowers, Weigela subsessilis , Wisteria sinensis , Wisteria sinensis , Zanthoxylum schinifolium , Corn flower Zea mays , Zingiber mioga , Zinnia elegans Provided is a flower mixed extract having antioxidant and whitening activity extracted by adding any one or a mixture thereof.

In addition, the present invention is an extraction method of the flower mixture extract having antioxidant and whitening activity,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Water or 2 to 100 times the weight of the raw material, or any one or a mixture of methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin An extraction step of putting the raw material in the extraction by heating for 30 minutes to 24 hours at 45 ~ 120 ℃; And

The extracted raw material is removed by filtration and water or methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or any mixture of two or more thereof is evaporated. It provides a method of extracting the flower mixture extract having antioxidant and whitening activity, including; filtration drying step of obtaining a dried solid extract.

In addition, the present invention is an extraction method of the flower mixture extract having antioxidant and whitening activity,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Immersion step of immersing the raw material in 30 to 98% methanol, ethanol, ethyl acetate or butanol aqueous solution or hexane or methylene chloride corresponding to 2 to 5 times the weight of the raw material;

Extraction step of proceeding 2 to 5 times the process of shaking the extraction of the result of the immersion step at 5 ~ 40 ℃ for 30 minutes to 15 days or shaking extraction for 1 to 5 days; And

Filtering and removing the raw material extracted through filtration and evaporating an aqueous methanol, ethanol, ethyl acetate, or butanol solution or hexane or methylene chloride to obtain a dried solid extract, and having an antioxidant and whitening activity. It provides a method of extracting the flower mixture extract.

In addition, the present invention is an extraction method of the flower mixture extract having antioxidant and whitening activity,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Immersion step of immersing the raw material in 20 to 70% butylene glycol, propylene glycol, dipropylene glycol or glycerin aqueous solution corresponding to 10 to 50 times the weight of the raw material;

An extraction step of proceeding with 1 to 3 repetitions of shaking the extract of the result of the immersion step at 5 to 40 ° C. for 30 minutes to 15 days or shaking for 5 to 7 days; And

It provides a method of extracting the flower mixture extract having antioxidant and whitening activity, including; filtration step of removing the raw material extracted through filtration to obtain an extract in a liquid state.

In addition, the present invention is a cosmetic composition,

It provides a cosmetic composition comprising a cosmetic base in the amount of 0.01 to 10% by weight and the balance of the flower mixture extract having the antioxidant and whitening activity.

Flower mixture extract having antioxidant and whitening activity of the present invention, extracting method thereof and cosmetic composition containing the flower mixture extract remove skin oxides to protect skin, prevent aging and inhibit the production of melanin in skin cells The superior effect of helping to whitening, the cosmetic composition prepared by adding the extract is also excellent in antioxidant and whitening effect.

The present invention is a flower mixture extract having antioxidant and whitening activity, the flower mixture extract having antioxidant and whitening activity,

Adonis amurensis, Akebia quinata, Albizia julibrissin , Althaea rosea , Angelica acutiloba, Angelica anomala , Aquilegia buergeriana , ssukkkot (Artemisa princeps), gaessukbujaengyi flowers (Aster ciliosus), Aster scaber flowers (Aster scaber), rape flower (Brassica campestris), mulberry flowers (Broussonetia kazinok), gwakkot (Callistephus chinensis), Camellia (Camellia japonica), tea flowers (Camellia sinensis , Campsis grandiflora , Calendula ( Caragana sinica ), Safflower ( Carthamus tinctorius ), Cassia obtusifolia , Cockscomb ( Celosia cristata ), Cercis chinensis , Chaenomeles sinensis , Persimmon (Chrysanthemum indicum), gujeolcho flowers (Chrysanthemum zawadskii), true euahri flowers (Clematis turniflora), dayflower flowers (Commelina communis), wild strawberry tree flower (Cornus kousa), Cornus officinalis flower (Cornus officinalis), often goebul pockets flowers (Cor ydalis incisa), Corydalis flower (Corydalis turtschaninovii), hobakkkot (Cucurbita moschate), nankkot (Dendrobium nobile), Dianthus (Dianthus chinensis), enjoy flowers (Elsholtzia splendens), Bone Spine herbs flowers (Eupatorium lindleyanum), forsythia flowers (Forsythia koreana , Fritillaria ussuriensis , Gardenia jasminoides , Ginkgo biloba , Helianthus annuus , Helianthus tuberosus , Hemerocallis fulva , Hibiscus mutabilis ), Hibisicus syriacus , Daylily ( Hosta plantaginae ), Hydrangea ( Hydrangea macrophylla ), Impatiens balsamina , Iris pallasii , Iris pseudacorus , Yellow plum ( Kerria japonica ) tree flower (Lagerstroemia indica), flowers motherwort (Leonurus sibiricus), Hagi tree flowers (Lespedeza bicolor), true lilies (Lilium lancifolium), ginger tree flower (Lindera obtusiloba), maekmundong flowers (Liriope muscari), honeysuckle (Loni cera japonica), the fall lobster Whatsoever flower (Lycoris radiata), Magnolia (Magnolia kobus), jamokryeon (Magnolia liliflora), pear blossoms (Malus sieboldii), daffodils (Narcissus tazetta), lotus (Nelumbo nucifera), evening primrose (Oenothera biennis), peony flower (Paeonia lactiflora), peonies (Paeonia suffruticosa), one yanggwibikkot (Papaver rhoeas), matari flowers (Patrinia scabiosifolia), paulownia flowers (paulownia coreana), gomanyi flower (Persicaria thunbergii), coltsfoot flowers (Petasites japonicus), morning glory (Pharbitis nil ), phytolacca esculenta , bellflower ( Platycodon grandiflorus ), oleander ( Polygonatum odoratum ), tangerine ( Poncirus trifoliata ), apricot ( Prunus armeniaca ), plum ( Prunus mume ), iris ( Prunus persica ), Prunus serrulata , Prunus tomentosa , Pueraria lobata , Rhododendron mucronulatum , Acacia ( Robinia pseudacacia ), Rosa multiflora , Rosa rugosa , Rose flower ( Sasa borealis) ), Sophora japonica , Spiraea prunifolia , Styrax japonica , Syringa vulgaris , Taraxacum platycarpum, Tulipa edulis , Viola mandschurica Water or organic in a mixture of two or more of the flowers, Weigela subsessilis , Wisteria sinensis , Wisteria sinensis , Zanthoxylum schinifolium , Corn flower Zea mays , Zingiber mioga , Zinnia elegans Extract with either solvent or mixtures thereof.

The flower mixture extract having antioxidant and whitening activity has an antioxidant effect of free radical scavenging activity and superoxide dismutase-like activity and a whitening effect of inhibiting tyrosinase activity and inhibiting melanin biosynthesis in mouse-derived melanoma cells.

The antioxidant effect of the flower mixture extract was demonstrated through measurement of free radical scavenging activity and measurement of superoxide dismutase (SOD) -like activity, and the whitening effect was measured by tyrosinase activity inhibition and melanin biosynthesis using B16F1 melanoma. Proven by inhibition measures.

The free radical scavenging activity is used as an indicator of antioxidant activity by measuring the absorbance after adding DPPH (1-1-diphenyl-2-picryl-hydrazyl) to the antioxidant effect of the flower mixture extract.

The superoxide dismutase (SOD) -like activity measurement was performed by adding a superoxide dismutase (SOD) assay kit to the flower mixture extract solution and measuring the absorbance to use as an indicator of antioxidant activity.

In the method of measuring tyrosinase activity inhibition, a method such as Yagi for measuring DOPA chrome produced by the action of tyrosinase by colorimetry is measured.

In the case of measuring the inhibition of melanin biosynthesis using the B16F1 melanoma, the effect of inhibiting melamine biosynthesis in the mouse-derived B16F10 melanoma is measured.

In addition, the flower mixture extract having antioxidant and whitening activity is extracted by heating the flower mixture in any one of the water or the organic solvent or a mixture thereof and heating at 45 to 120 ℃ for 30 minutes to 24 hours, or the flower mixture It is obtained by immersing in the organic solvent alone or a mixture of the water and the organic solvent for 30 minutes to 15 days at 5 ~ 40 ℃.

In this case, the organic solvent may be any one of methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin, or a mixture of two or more thereof.

The extracting organic solvent includes a hydrophilic organic solvent alcohol, glycol, glycerin and lipophilic organic solvent hexane, methylene chloride, ethyl acetate in order to extract both the hydrophilic and lipophilic extract of the flower mixture extract.

In addition, the extraction method of the flower mixture extract having the antioxidant and whitening activity of the present invention,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Water or 2 to 100 times the weight of the raw material, or any one or a mixture of methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin An extraction step of putting the raw material in the extraction by heating for 30 minutes to 24 hours at 45 ~ 120 ℃; And

The extracted raw material is removed by filtration and water or methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or any mixture of two or more thereof is evaporated. It comprises; filtration drying step to obtain an extract of the dried solid state.

Here, the extraction method of the flower mixture extract having the antioxidant and whitening activity is when the water is used as the extraction solvent, the flower mixture as a raw material for 30 minutes to 24 hours more than 2 to 100 times the weight of the flower mixture It is extracted by heating to 45 ~ 120 ℃ in water.

In addition, when the organic solvent is used as an extraction solvent, the flower mixture, which is a raw material, is added to the organic solvent at least 2 to 100 times the weight of the flower mixture for 30 minutes to 24 hours, and extracted by heating to 45 to 120 ° C. .

The extractant is then evaporated to give an extract in the form of a dried solid.

Alternatively, the extracted raw material is removed through filtration to obtain a liquid extract therefrom.

At this time, if the weight of the extraction solvent is less than 2 times the weight of the flower mixture, the extract cannot be extracted sufficiently. If the extraction solvent is too large, the extraction solvent is too large and time and cost are high to obtain the dry extract. You will hear In the case of water, it is necessary to carry out the extraction in a heated state in order to sufficiently include the kinds of extracting substances, and to increase the extraction efficiency. If the temperature is too low, it is difficult to take full advantage of the warming, and the extraction time is too high. Decomposition of useful substances may occur.

If the extraction time is less than 30 minutes, sufficient extraction cannot be achieved, and if the extraction time is excessively greater than 24 hours, the extraction efficiency is lowered, thereby making the process inefficient.

In addition, the extraction method of the flower mixture extract having the antioxidant and whitening activity of the present invention,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Immersion step of immersing the raw material in 30 to 98% methanol, ethanol, ethyl acetate or butanol aqueous solution or hexane or methylene chloride corresponding to 2 to 5 times the weight of the raw material;

Extraction step of proceeding 2 to 5 times the process of shaking the extraction of the result of the immersion step at 5 ~ 40 ℃ for 30 minutes to 15 days or shaking extraction for 1 to 5 days; And

Filtration drying step to remove the raw material extracted through filtration and to evaporate an aqueous methanol, ethanol, ethyl acetate, or butanol solution or hexane or methylene chloride to obtain an extract of the dried solid state.

That is, when 30 to 98% methanol, ethanol, ethyl acetate or butanol aqueous solution or hexane or methylene chloride is used as an extraction solvent, the flower mixture as a raw material is 30 to 98% of 2 to 5 times the weight of the flower mixture. Methanol, ethanol, ethyl acetate or butanol aqueous solution or hexane or methylene chloride in a solvent extraction for 30 minutes to 15 days at 30 ~ 15 days shaking or extracting for 2 to 5 times the shaking extraction for 1 to 5 days do.

The extractant is then evaporated to give an extract in the form of a dried solid.

In this case, when the extraction solvent contains alcohols such as ethanol, the extraction solvent can be extracted at room temperature, and the powdered sample is immersed in a sufficient extraction solvent for a sufficient time, for example, about 3 days so that the extraction is sufficient. If the extraction is not sufficient, the extraction amount may be increased by an iterative process or by increasing the extraction time. In particular, in the case of extraction of alcohol, when the extraction proceeds, the method of concentrating the extract first with a rotary vacuum evaporator to remove the extraction solvent and then freeze-drying may be used. It is common to first filter out impurities.

In addition, the extraction method of the flower mixture extract having the antioxidant and whitening activity of the present invention,

Preparing raw materials by steaming the flowers harvested for each period as it is or steaming;

Immersion step of immersing the raw material in 20 to 70% butylene glycol, propylene glycol, dipropylene glycol or glycerin aqueous solution corresponding to 10 to 50 times the weight of the raw material;

An extraction step of proceeding with 1 to 3 repetitions of shaking the extract of the result of the immersion step at 5 to 40 ° C. for 30 minutes to 15 days or shaking for 5 to 7 days; And

And a filtering step of removing the extracted raw material through filtration and obtaining an extract in a liquid state therefrom.

That is, when 20 to 70% of butylene glycol, propylene glycol, dipropylene glycol, or glycerin aqueous solution is used as an extraction solvent, the flower mixture, which is a raw material, is 20 to 70% of 10 to 50 times the weight of the flower mixture. Butyl glycol, propylene glycol, dipropylene glycol or glycerin aqueous solution in a solvent extraction solution of the immersion step for 30 minutes to 15 days or shake extraction for 5 to 7 days at 1 ~ 3 Extract repeatedly.

Then, the extracted raw material is removed by filtration to obtain a liquid extract therefrom.

In this case, when the extraction solvent contains a polyol such as butylene glycol, it can be extracted at room temperature, so that the powder is immersed in a sufficient extraction solvent for a sufficient time, for example, about 3 days so that the extraction is sufficient. If the extraction is not sufficient, the extraction amount may be increased through an iterative process or by increasing the extraction time.

In addition, the cosmetic composition of the present invention comprises a cosmetic base in an amount of 0.01 to 10% by weight and the balance of the flower mixture extract having the antioxidant and whitening activity.

The cosmetic composition has antioxidant and whitening activity by containing the flower mixture extract having antioxidant and whitening activity.

Here, when the content of the cosmetic composition exceeds the above range shows the saturation of the antioxidant and whitening effect, the effect of the content can be reduced, so it is effective to include less than that, if less than the above range is sufficient to the antioxidant and whitening effect Can not get

Here, the cosmetic base is a cosmetic base for forming a flexible cosmetics, nourishing cosmetics, nutrition essence, nutrition cream, body cream or pack.

Each of the cosmetic base forms a flexible cosmetic water, nutrient cosmetics, nutrition essence, nutrition cream, body cream or pack with the flower mixture extract.

That is, the cosmetic composition using the flower mixture extract is a flexible cosmetics, nourishing cosmetics, nutrition essence, nutrition cream, body cream or pack.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract having the antioxidant and whitening activity and a softening longevity base as the softening longevity, 0.01 ~ 10% by weight of the flower mixed extract, polyoxyethylene hardened castor oil as the softening longevity base 0.01 to 1.0% by weight, glycine 2 to 5% by weight, dipotassium glycylizate 0.01 to 3% by weight, 1,3-butylene glycol 0.5 to 8% by weight, sodium hyaluronate 0.01 to 3% by weight, ethanol 3 ~ 10% by weight, triethanolamine 0.01 ~ 3% by weight, EDTA 0.01 ~ 3% by weight and the balance includes antioxidant and purified water.

The softening lotion includes skin lotion and toner.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract and nutrient cosmetics base having the antioxidant and whitening activity as nutrient cosmetics, 0.01 ~ 10% by weight of the flower mixture extract, and 3 ~ 9% by weight glycerin as a nutrient cosmetics base , Sorbitan stearate sucrose cocoate 0.2 to 8% by weight, mineral oil 0.5 to 8% by weight, trioctanoin 0.2 to 5% by weight, stearic acid 0.2 to 5% by weight, glyceryl stearate 0.02 to 3 Wt%, sorbitan monostearate 0.2-5 wt%, carbomer 0.2-5 wt%, dimethicone 0.02-3 wt%, triethanolamine 0.01-3 wt%, EDTA 0.01-3 wt% and the balance with antioxidant Includes purified water.

The nutritional cosmetics include nutrition lotion.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract and a nutrient essence base having the antioxidant and whitening activity as a nutrient essence, 0.01 ~ 10% by weight of the flower mixture extract, 0.2 ~ 7% by weight glycerin as a nutrient essence base , 1,3 butylene glycol 0.2-5 wt%, polyethylene glycol 0.2-5 wt%, carbomer 0.2-5 wt%, sodium hyaluronate 0.02-3 wt%, glycine 0.2-7 wt%, polyacrylamide 0.2 ~ 5% by weight, hydroxyethyl cellulose 0.02 ~ 3% by weight, polyoxyethylene cured castor oil 0.02 ~ 8% by weight, ethanol 0.2 ~ 7% by weight, triethanolamine 0.01 ~ 3% by weight, EDTA 0.01 ~ 3% by weight And remaining amounts of antioxidants and purified water.

The nutritional essence includes an essence, a cleansing essence.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract and a nutrient cream base having the antioxidant and whitening activity as a nourishing cream, 0.01 ~ 10% by weight of the flower mixture extract, 1,3 butylene glycol as a nutrient cream base 0.2-7 wt%, Glycerin 0.2-7 wt%, Hydrogenated lecithin 0.3-5 wt%, Octyldodecanol 0.2-7 wt%, Trioctanoin 0.2-5 wt%, Stearic acid 0.2-5 wt% %, Cetostearyl alcohol 0.2-5 wt%, polysorbate 60 0.2-7 wt%, sorbitan sesquioleate 0.2-5 wt%, dimethicone 0.2-8 wt%, xanthan gum 0.02--3 wt%, triethanol 0.01 to 3% by weight of amine, 0.01 to 3% by weight of EDTA and the balance include antioxidant and purified water.

The nutrition cream includes a cream, cleansing cream.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract and a body cream base having the antioxidant and whitening activity as a body cream, 0.01 to 10% by weight of the flower mixture extract, 1,3 butylene glycol as a body cream base 0.5 to 9% by weight, glycerin 0.5 to 8% by weight, sodium cetyl sulfate 0.03 to 3%, pantanol 0.2 to 5% by weight, carbomer 0.02 to 3% by weight, biswax 0.02 to 3% by weight, cetanol 0.2 to 7% by weight, glyceryl stearate 0.2-7%, sorbitan stearate 0.2-5%, mineral oil 0.5-9%, squalene 0.2-5%, dimethicone 0.2-5%, triethanolamine 0.01 ˜3% by weight, EDTA 0.01-3% by weight and the balance include antioxidant and purified water.

The body cream includes a keratin care cream.

In addition, the cosmetic composition of the present invention is composed of a flower mixture extract and a pack base having the antioxidant and whitening activity as a pack, 0.01 to 10% by weight of the flower mixture extract, glycerin 3 to 9% by weight, 1, 3 butylene glycol 0.2-5 wt%, squalene 0.2-5 wt%, dimethicone 0.2-5 wt%, sodium hyaluronate 0.03-3 wt%, glycine 0.2-7 wt%, polyacrylamide 3-9 wt% 0.01 to 3% by weight of triethanolamine, 0.01 to 3% by weight of EDTA and the balance include antioxidant and purified water.

The pack includes a face pack, a body pack, and a sleep pack.

In other words, the present invention focuses on the various ingredients and efficacy of these flowers to produce mixed extracts of these ingredients, thereby developing cosmetic raw materials that maximize the useful ingredients for the skin, creating new added value and contributing to the horticulture industry Cosmetics useful for skin health have been developed.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

Example 1 Extraction Method of Flower Mixture Extract Using Water as Extraction Solvent

First, 0.1 kg of a flower mixture sample is prepared in order to obtain a water extract of a mixture of a periwinkle flower, a taegi flower, a magnolia flower, and an acacia flower in a ratio of 1: 1: 1: 1. The sample of the flower mixture is placed in purified water 5 times by weight and extracted by heating at 105 ° C for 5 hours. At the end of the extraction process, the filter was filtered with Whatman paper No. 1, and then concentrated using a rotary vacuum evaporator to remove water, followed by freeze drying. This obtained 21.42 g (dry weight) of flower mixture extract.

Example 2. Extraction Method of Flower Mixture Extract Using 80% Methanol as Extraction Solvent

Three times the extraction solvent (80% methanol) was added to 0.1 kg of the flower mixture, which was mixed at a ratio of 1: 1: 1: After three times of shaking extraction process, the extract was filtered through a filter paper, concentrated with a rotary vacuum evaporator (rotary vacuum evaporator) and freeze-dried to obtain a flower mixture 80% methanol extract 29.07g (dry weight).

Example 3. Extraction Method of Flower Mixture Extract Using 95% Ethanol as Extraction Solvent

Three times the extraction solvent (95% ethanol) was added to 0.1 kg of the flower mixture sample, which was mixed at 1: 1: 1: 1 ratio of guinea-earflower, taegi-flower, magnolia flower, and acacia flower, respectively, at 25 ° C for 72 hours. After three times of shaking extraction process, the extract was filtered with a filter paper, concentrated with a rotary vacuum evaporator (rotary vacuum evaporator) and freeze-dried to obtain a flower mixture 95% ethanol extract 5.24g (dry weight).

Example 4 Extraction Method of Flower Mixture Extract Using 70% Ethanol as Extraction Solvent

Three times the extraction solvent (70% ethanol) was added to 0.1 kg of the flower mixture sample, which was mixed at 1: 1: 1: 1 ratio of guinea pig flower, baktaegi flower, magnolia flower and acacia flower, respectively, at 25 ° C for 72 hours. After shaking three times, the extract was filtered with a filter paper, concentrated with a rotary vacuum evaporator, and freeze-dried to obtain 36.12 g (dry weight) of a 70% ethanol extract of the flower mixture.

Example 5 Extraction Method of Flower Mixture Extract Using 30% Butylene Glycol as Extraction Solvent

50 times of extraction solvent (30% butylene glycol) was added to 0.1 kg of the flower mixture sample, which was mixed 1: 1 and 1: 1, respectively, with one-day ear flower, park taegi flower, magnolia flower and acacia flower. After one day of shaking extraction, the extract was filtered with a filter paper, concentrated with a rotary vacuum evaporator, and lyophilized to give 4.284 kg (30% solids) of the flower mixture 30% butylene glycol extract. Got it.

Example 6 Extraction Method of Flower Mixture Extract Using 50% Butylene Glycol as Extraction Solvent

50 times of extraction solvent (50% butylene glycol) was added to 0.1 kg of the flower mixture sample, which was mixed 1: 1, 1: 1, and iris flower, taegi flower, magnolia flower, and acacia flower, respectively. After one day of shaking extraction, the extract was filtered with a filter paper, concentrated with a rotary vacuum evaporator, and freeze-dried to obtain 3.491 kg of a 30% butylene glycol extract mixed with flowers (about 2.5% solids). Got it.

Example 7 Extraction Method of Flower Mixture Extract Using 50% Propylene Glycol as Extraction Solvent

50 times of extraction solvent (50% propylene glycol) was added to 0.1kg of the flower mixture sample, which was mixed 1: 1, 1: 1, and iris flowers, taegi flowers, magnolia flowers, and acacia flowers, respectively, at 25 ° C for 5 days. After the shaking extraction process was performed once, the extract was filtered with a filter paper, concentrated with a rotary vacuum evaporator, and freeze-dried to obtain 3.621 kg of a flower-mixed 30% propylene glycol extract (about 3.1% solids).

Experimental Example 1

Antioxidative effect was measured for each extract sample obtained by the method of Examples 4 and 5, which is determined to be the most effective in the extraction process and efficiency among the above examples.

1-1. Free radical scavenging capacity measurement

1-1-1. Experiment method

The electron donating ability (the ability to supply electrons) for the sample was measured as follows. 5 × 10 ^-4 M of DPPH (1-1-diphenyl-2-picryl-hydrazyl) 100 was added to a solution 100 of each concentration of each extract, stirred for 1 minute, and then reacted in a 25 incubator for 30 minutes, followed by a microplate. Absorbance was measured at 540 nm using a counter.

El-ascorbic acid (1,000µg / ml) was used as an experimental control, and the electron donating ability was expressed as the absorbance reduction rate of the sample solution added and the no added solution.

1-1-2. Experiment result

The results are shown in Tables 1 and 2 below and are shown graphically in FIGS. 1 and 2.

sample
(Μg / ml) 10000 1000 750 500 250 100 10 One Example 4 93.0% 92.7% 93.1% 88.8% 55.1% 21.0% 1.8% 3.8%

sample
(%) 100 50 10 5 4 3 2 One Example 5 92.9% 93.2% 92.5% 70.8% 61.1% 48.9% 36.2% 20.4%

As shown in Table 1 and Table 2 and Figures 1 and 2, Example 4, Example 5 showed a high free radical scavenging ability and about 230 (㎍ / ㎖) and Example for the ethanol extract of Example 4 The butylene glycol extract of 5 exhibited an IC ₅₀ of about 3.2%, indicating an effect of inhibiting cell damage and skin aging progression due to free radicals. The control group, L-ascorbic acid (1,000 μg / ml), exhibited 95% scavenging activity.

1-2. Superoxide dismutase (SOD) -like activity measurement

1-2-1. Experiment method

SOD-like activity was performed as follows. 20 ml of sample solution for each concentration of 100 ppm of each extract was added with 200 WST working solution in a superoxide dismutase assay kit (SOD assay kit-WST), and then stirred again. working solution) 20 μl of the solution was mixed and reacted in a 37 ° C. incubator for 20 minutes, and the absorbance was measured at 450 nm using a microplate counter.

Superoxide dismutase (1,000units) was used as the experimental control, and superoxide dismutase-like activity was expressed as the absorbance reduction rate of the sample solution added and the no added solution.

1-2-2. Experiment result

The results are shown in Tables 3 and 4 below, and are shown graphically in FIGS. 3 and 4.

sample
(Μg / ml) 250 125 62.5 Example 4 21.19% 11.68% 6.63%

sample
(%) 100 50 10 5 4 3 2 One Example 5 100.5% 100.1% 89.7% 71.8% 65.9% 56.1% 40.3% 21.4%

As shown in Table 3, Table 4, and Figures 3 and 4, Example 4, Example 5 is all excellent in the superoxide discutase-like activity, in particular, in the case of the butylene glycol extract of Example 5, The activity was higher than that of the ethanol extract of Example 4, showing excellent antioxidant and anti-aging effects. In comparison, the superoxide dismutase (1,000 units) showed 99.5% of activity.

Experimental Example 2

The whitening effect of Example 4 and Example 5 was measured.

2-1. Determination of Tyrosinase Enzyme Inhibitory Activity

2-1-1. Experiment method

Tyrosinase inhibitory activity was measured by modifying a method such as Yagi, which measures dopa chromium produced by the action of tyrosinase by colorimetry. That is, the reaction port was 40 μl of 1.5 mM L-Tyrosine solution (in 0.1 M phosphate buffer (pH 6.5) and sample in 110 μl of 0.1 M phosphate buffer (pH6.5) in a 96-well plate. After 10 µl of the solution was added, 10 µl of mushroom tyrosinase (2000 units / ml) was added thereto, and reacted at 37 ° C. for 10 minutes to measure dopachrome at 490 nm using a microplate counter.

The same concentration of kojic acid was used as a positive control for comparison.

2-1-2. Experiment result

The results are shown in Table 5 below and graphically shown in FIG. 5.

sample
(%) 10 5 2.5 One 0.5 Example 5 35.31% 18.92% 11.87% 6.09% 2.10%

As shown in Table 5 and Figure 5, in the case of Example 5, it can be seen that the tyrosinase-like activity shows an excellent effect by increasing the concentration significantly. However, in the case of Example 4, the low activity was shown that Example 5 is a better tyrosinase activity inhibitor. In the case of the control group kojic acid (1,000 μg / ml), the activity was 93.3%.

2-2. Determination of Melanin Biosynthesis Inhibition in B16F10 Melanoma Cells

2-2-1. Experiment method

In order to determine the effect of inhibiting melamine (melanin) biosynthesis in the mouse-derived B16F10 melanoma was carried out as follows. That is, after culturing B16F10 (melanoma) in 10% FBS-MEME, the cell solution was prepared by adjusting the cell concentration to 5.0 × 10 ⁴ cells / ml, and then, each 5 ml was dispensed into a 60 mm diameter plate. Incubated for 24 hours in a 37, 5% CO ₂ incubator. After incubation for 24 hours, after removing the medium, 1 ml of a 500 ppm sample prepared with 10% FBS-MEME and 4 ml of 5% Theophylline (0.05M) (in 10% FBS-MEME) were added again. Incubated for 72 hours under the same conditions. At this time, the experiment using only 10% FBS-MEME was used as a control, the positive control was used arbutin of the same concentration. After incubation for 72 hours, in order to measure the amount of melanin in solution in each plate, 1 ml of the solution was transferred to a microtube, 200 μl of the solution was dispensed into a 96-well plate, and then, using a microplate counter. Absorbance was measured at 405 nm. In addition, in order to measure intracellular melanin production, the remaining medium in the plate was first removed and washed once with 1 × PBS (−). 0.5 ml of 0.85N potassium hydroxide solution was added again, and then reacted for about 30 minutes to 1 hour to take intracellular melanin and transferred to the microtube. Then, 0.5 ml of 1 × PBS (-) was added and the remaining amount of melanin was taken and mixed. Also, 200 μl each was taken from the plate and transferred to a 96-well plate, and the absorbance was measured at 405 nm using a microplate counter.

2-2-2. Experiment result

The results are shown in Table 6 below and graphically shown in FIG. 6.

sample Arbutin (µg / mL) Example 4 (μg / ml) Example 5 (%) 250 100 50 250 100 50 2.5 1.0 0.5 Inhibition rate (%) 41.9 20.1 6.3 25.3 11.9 5.3 39.7 18.7 8.6

As shown in Table 6 and Figure 6, in the case of Example 4 and Example 5, both of the B16F1 melanoma cells in the melanin biosynthesis showed a significant concentration-dependent inhibition of the whitening effect was excellent. In particular, in the case of Example 5, the whitening effect was excellent enough to show a similar effect to the comparison group arbutin.

Prescription example of cosmetic composition

The following shows an example of the formulation of the cosmetic composition as a formulation having a skin protection and whitening effect through the effect of removing the active oxygen in the skin and inhibiting the production of the skin melanin by containing the flower extract in each cosmetic formulation. As follows. The flower mixture extract used here is that of Example 5 which is the best in antioxidant and skin whitening.

Formulation Example 1. Soft Cosmetics

Prescription examples of the flexible cosmetics in the cosmetic containing the flower mixture extract according to the present invention are shown in Table 7.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 1.0 Polyoxyethylene Cured Castor Oil 0.4 Glycine 3.5 Dipotassium glycylizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Purified water Balance (to 100)

Prescription Example 2. Nutritional Cosmetics

Prescription example of nutritional longevity in cosmetics containing the flower mixture extract according to the present invention is shown in Table 8.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 1.0 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 Purified water Balance (to 100)

Prescription Example 3. Nutrition Essence

Prescription examples of nutritional essences of cosmetics containing the flower mixture extract according to the present invention are shown in Table 9 below.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 3.0 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaluronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl Cellulose 0.2 ethanol 3.0 Polyoxyethylene Cured Castor Oil 1.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Purified water Balance (to 100)

Prescription 4. Nutritional Cream

Prescription example of nutrition cream in the cosmetic composition containing the flower mixture extract according to the present invention is shown in Table 10 below.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 5.0 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan Gum 0.2 Triethanolamine 0.1 EDTA 0.1 Purified water Balance (to 100)

Prescription Example 5. Body Cream

Prescription examples of body creams in the cosmetic composition containing the flower mixture extract according to the present invention are shown in Table 11 below.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 3.0 1,3-butylene glycol 6.0 glycerin 4.0 Sodium cetyl sulfate 0.1 Panthenol 1.0 Xanthan Gum 1.0 Carbomer 0.1 Biswax 0.3 Cetanol 2.5 Glyceryl Stearate 2.5 Sorbitan stearate 1.5 Mineral oil 5.0 Squalene 2.0 Antioxidant 0.3 Dimethicone 1.0 Triethanolamine 0.1 EDTA 0.1 Purified water Balance (to 100)

Prescription 6.Pack

Prescription examples of the pack of cosmetics containing the flower mixture extract according to the present invention are shown in Table 12 below.

ingredient Content (% by weight) Flower Mixed Extract (Example Method 5) 5.0 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyaluronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Purified water Balance (to 100)

As confirmed in the above examples and experimental examples, the flower mixture extract according to the present invention has excellent antioxidant and skin whitening effect, and the cosmetic composition prepared by adding the extract of the present invention also has an antioxidant and whitening effect.

Therefore, according to the present invention, there is an effect of providing a flower composition extract and a cosmetic composition comprising the same, which inhibits oxidation to prevent skin cell damage and aging and inhibits the production of melanin in the skin cell to help whitening.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

1 is a graph showing the antioxidant effect through the free radical scavenging ability while varying the concentration of the flower mixture extract obtained in Example 4 of the present invention.

Figure 2 is a graph showing the antioxidant effect through the free radical scavenging ability while changing the flower mixture extract obtained in Example 5 of the present invention by concentration.

Figure 3 is a graph showing the results of testing the antioxidant effect by measuring the SOD-like activity by changing the flower mixture extract extract obtained in Example 4 of the present invention.

Figure 4 is a graph showing the results of testing the antioxidant effect by measuring the SOD-like activity while changing the flower mixture extract extract obtained in Example 5 of the present invention.

5 is a graph showing the results of experiments on the whitening effect by measuring the tyrosinase activity inhibitory effect while changing the flower mixture extract extract obtained in Example 5 of the present invention.

Figure 6 is a graph showing the results of experiments whitening effect by measuring the melanin biosynthesis inhibitory effect in B16F1 melanoma while varying the concentration of the flower mixture extract extract obtained in Examples 4 and 5 of the present invention.

Claims (14)

Adonis amurensis, Akebia quinata, Albizia julibrissin , Althaea rosea , Angelica acutiloba, Angelica anomala , Aquilegia buergeriana , ssukkkot (Artemisa princeps), gaessukbujaengyi flowers (Aster ciliosus), Aster scaber flowers (Aster scaber), rape flower (Brassica campestris), mulberry flowers (Broussonetia kazinok), gwakkot (Callistephus chinensis), Camellia (Camellia japonica), tea flowers (Camellia sinensis , Campsis grandiflora , Calendula ( Caragana sinica ), Safflower ( Carthamus tinctorius ), Cassia obtusifolia , Cockscomb ( Celosia cristata ), Cercis chinensis , Chaenomeles sinensis , Persimmon (Chrysanthemum indicum), gujeolcho flowers (Chrysanthemum zawadskii), true euahri flowers (Clematis turniflora), dayflower flowers (Commelina communis), wild strawberry tree flower (Cornus kousa), Cornus officinalis flower (Cornus officinalis), often goebul pockets flowers (Cor ydalis incisa), Corydalis flower (Corydalis turtschaninovii), hobakkkot (Cucurbita moschate), nankkot (Dendrobium nobile), Dianthus (Dianthus chinensis), enjoy flowers (Elsholtzia splendens), Bone Spine herbs flowers (Eupatorium lindleyanum), forsythia flowers (Forsythia koreana , Fritillaria ussuriensis , Gardenia jasminoides , Ginkgo biloba , Helianthus annuus , Helianthus tuberosus , Hemerocallis fulva , Hibiscus mutabilis ), Hibisicus syriacus , Daylily ( Hosta plantaginae ), Hydrangea ( Hydrangea macrophylla ), Impatiens balsamina , Iris pallasii , Iris pseudacorus , Yellow plum ( Kerria japonica ) tree flower (Lagerstroemia indica), flowers motherwort (Leonurus sibiricus), Hagi tree flowers (Lespedeza bicolor), true lilies (Lilium lancifolium), ginger tree flower (Lindera obtusiloba), maekmundong flowers (Liriope muscari), honeysuckle (Loni cera japonica), the fall lobster Whatsoever flower (Lycoris radiata), Magnolia (Magnolia kobus), jamokryeon (Magnolia liliflora), pear blossoms (Malus sieboldii), daffodils (Narcissus tazetta), lotus (Nelumbo nucifera), evening primrose (Oenothera biennis), peony flower (Paeonia lactiflora), peonies (Paeonia suffruticosa), one yanggwibikkot (Papaver rhoeas), matari flowers (Patrinia scabiosifolia), paulownia flowers (paulownia coreana), gomanyi flower (Persicaria thunbergii), coltsfoot flowers (Petasites japonicus), morning glory (Pharbitis nil ), phytolacca esculenta , bellflower ( Platycodon grandiflorus ), oleander ( Polygonatum odoratum ), tangerine ( Poncirus trifoliata ), apricot ( Prunus armeniaca ), plum ( Prunus mume ), iris ( Prunus persica ), Prunus serrulata , Prunus tomentosa , Pueraria lobata , Rhododendron mucronulatum , Acacia ( Robinia pseudacacia ), Rosa multiflora , Rosa rugosa , Rose flower ( Sasa borealis) ), Sophora japonica , Spiraea prunifolia , Styrax japonica , Syringa vulgaris , Taraxacum platycarpum, Tulipa edulis , Viola mandschurica , Flower mixture of Weigela subsessilis , Wisteria sinensis , Wisteria sinensis , Zanthoxylum schinifolium , Corn flower Zea mays , Zingiber mioga and Zinnia elegans in water or organic solvents. A mixed flower extract having antioxidant and whitening activity extracted by adding any one or a mixture thereof. The method of claim 1, wherein the organic solvent is any one of methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof. Flower mixture extract having antioxidant and whitening activity. According to claim 1 or claim 2, wherein the antioxidant and whitening activity of the flower mixture extract is the flower mixture of any one of the water or the organic solvent or in a mixture thereof 30 minutes to 24 hours at 45 ~ 120 ℃ Extracted by heating, or a mixture of flowers having antioxidant and whitening activity, characterized in that obtained by dipping the flower mixture in the organic solvent alone or a mixture of water and the organic solvent for 30 minutes to 15 days at 5 ~ 40 ℃. . Preparing raw materials by steaming the flowers of claim 1 as they are or after steaming; Water or 2 to 100 times the weight of the raw material, or any one or a mixture of methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin An extraction step of putting the raw material in the extraction by heating for 30 minutes to 24 hours at 45 ~ 120 ℃; And The extracted raw material is removed by filtration and water or methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or any mixture of two or more thereof is evaporated. Filtration drying step of obtaining a dried solid extract to obtain a method of extracting the flower mixture extract with antioxidant and whitening activity comprising a. Preparing raw materials by steaming the flowers of claim 1 as they are or after steaming; Immersion step of immersing the raw material in 30 to 98% methanol, ethanol, ethyl acetate or butanol aqueous solution or hexane or methylene chloride corresponding to 2 to 5 times the weight of the raw material; Extraction step of proceeding 2 to 5 times the process of shaking the extraction of the result of the immersion step at 5 ~ 40 ℃ for 30 minutes to 15 days or shaking extraction for 1 to 5 days; And Filtering and removing the raw material extracted through filtration and evaporating an aqueous methanol, ethanol, ethyl acetate, or butanol solution or hexane or methylene chloride to obtain a dried solid extract, and having an antioxidant and whitening activity. Extraction method of flower mixture extract. Preparing raw materials by steaming the flowers of claim 1 as they are or after steaming; Immersion step of immersing the raw material in 20 to 70% butylene glycol, propylene glycol, dipropylene glycol or glycerin aqueous solution corresponding to 10 to 50 times the weight of the raw material; An extraction step of proceeding with 1 to 3 repetitions of shaking the extract of the result of the immersion step at 5 to 40 ° C. for 30 minutes to 15 days or shaking for 5 to 7 days; And A filtration step of removing the extracted raw material through filtration to obtain an extract in a liquid state therefrom; Extraction method of the flower mixture extract having antioxidant and whitening activity comprising a. A cosmetic composition comprising a cosmetic base in an amount of 0.01 to 10% by weight and the remaining amount of the flower mixture extract having the antioxidant and whitening activity according to claim 1. According to claim 7, wherein the cosmetic base is a cosmetic composition, characterized in that the cosmetic base for forming a flexible cosmetics, nourishing cosmetics, nutrition essence, nutrition cream, body cream or pack. Consisting of a flower mixture extract having the antioxidant and whitening activity as described in claim 1 and the flexible cosmetics base, and the flower mixture extract 0.01 ~ 10% by weight, and polyoxyethylene hardened castor oil 0.01 ~ 1.0 as the flexible cosmetics base Wt%, glycine 2-5 wt%, dipotassium glycylizate 0.01-3 wt%, 1-8 3-butylene glycol 0.5-8 wt%, sodium hyaluronate 0.01-3 wt%, ethanol 3-10 wt% %, Triethanolamine 0.01 to 3% by weight, EDTA 0.01 to 3% by weight and remaining amount of the cosmetic composition comprising an antioxidant and purified water. Consisting of the flower mixture extract having the antioxidant and whitening activity as described in claim 1 and nutrient cosmetics base as nutrient cosmetics, 0.01 ~ 10% by weight of the flower mixture extract, 3 ~ 9% by weight glycerin as nutrient cosmetics base, sorbitan 0.2-8 wt% of stearate sucrose cocoate, 0.5-8 wt% of mineral oil, 0.2-5 wt% of trioctanoin, 0.2-5 wt% of stearic acid, 0.02-3 wt% of glyceryl stearate, Sorbitan monostearate 0.2 to 5% by weight, carbomer 0.2 to 5% by weight, dimethicone 0.02 to 3% by weight, triethanolamine 0.01 to 3% by weight, EDTA 0.01 to 3% by weight and the remaining amount of antioxidants and purified water Cosmetic composition, characterized in that. As a nutritional essence consisting of the flower mixture extract and the nutritional essence base having the antioxidant and whitening activity of claim 1, the flower mixture extract 0.01 to 10% by weight, glycerin 0.2 to 7% by weight, 1, 3 butylene glycol 0.2-5 wt%, polyethylene glycol 0.2-5 wt%, carbomer 0.2-5 wt%, sodium hyaluronate 0.02-3 wt%, glycine 0.2-7 wt%, polyacrylamide 0.2-5 wt% %, Hydroxyethyl cellulose 0.02 to 3% by weight, polyoxyethylene cured castor oil 0.02 to 8% by weight, ethanol 0.2 to 7% by weight, triethanolamine 0.01 to 3% by weight, EDTA 0.01 to 3% by weight and the balance Cosmetic composition comprising an antioxidant and purified water. As a nourishing cream, it is composed of the flower mixed extract and the nourishing cream base having the antioxidant and whitening activity according to claim 1, wherein the flower mixed extract is 0.01 to 10% by weight, and 1,3 butylene glycol 0.2 to 7 as the nourishing cream base. Weight%, Glycerin 0.2-7%, Hydrogenated Lecithin 0.3-5%, Octyldodecanol 0.2-7%, Trioctata Elderly 0.2-5%, Stearic Acid 0.2-5%, Seto Stearyl alcohol 0.2-5 wt%, Polysorbate 60 0.2-7 wt%, Sorbitan sesquioleate 0.2-5 wt%, Dimethicone 0.2-8 wt%, Xanthan gum 0.02--3 wt%, Triethanolamine 0.01- Cosmetic composition comprising an antioxidant and purified water in 3% by weight, 0.01 to 3% by weight and EDTA. As a body cream, the flower mixture extract having the antioxidant and whitening activity as described in claim 1 and a body cream base, wherein the flower mixture extract 0.01 to 10% by weight, and as a body cream base 1,3 butylene glycol 0.5 to 9 Wt%, glycerin 0.5-8 wt%, sodium cetyl sulfate 0.03-3 wt%, pantanol 0.2-5 wt%, carbomer 0.02-3 wt%, biswax 0.02--3 wt%, cetanol 0.2-7 wt% , Glyceryl stearate 0.2-7 wt%, sorbitan stearate 0.2-5 wt%, mineral oil 0.5-9 wt%, squalene 0.2-5 wt%, dimethicone 0.2-5 wt%, triethanolamine 0.01-3 wt% Cosmetic composition, characterized in that it comprises antioxidant and purified water in%, 0.01 to 3% by weight and EDTA. A pack consisting of a flower mixture extract having the antioxidant and whitening activity as described in claim 1 and a pack base, 0.01 to 10% by weight of the flower mixture extract, and 3 to 9% by weight of glycerin as a pack base, 1,3 butylene Glycol 0.2-5%, Squalene 0.2-5%, Dimethicone 0.2-5%, Sodium Hyaluronate 0.03-3%, Glycine 0.2-7%, Polyacrylamide 3-9%, Triethanolamine Cosmetic composition, characterized in that it comprises an antioxidant and purified water in 0.01 to 3% by weight, EDTA 0.01 to 3% by weight and the balance.

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