KR20200132215A - The method for extracting fruit of wild strawberry and Cosmetic composition containing its seed - Google Patents

Abstract

The present invention relates to an invention related to a cosmetic Rubus crataegifolius body scrub composition containing extracts and seeds separated from Rubus crataegifolius fruits. A process of extracting a cosmetic extract from Rubus crataegifolius fruits comprises the following processes: 1) washing 10 kg of Rubus crataegifolius fruits in running water for 10 minutes; 2) after washing, adding 35 L of ethanol and 15 L of purified water, followed by stirring the same at 50°C for 5 hours to extract an extract; and 3) separating the extract by using a 20 μm sieve, and then freeze-drying the same to make into a powder, wherein a process of separating Rubus crataegifolius seeds from Rubus crataegifolius fruits comprises the following processes: 1) washing 10 kg of Rubus crataegifolius fruits in running water for 10 minutes; 2) after washing, adding 35 L of ethanol and 15 L of purified water, followed by stirring the same at 50°C for 5 hours to extract an extract; 3) separating extracts and seeds of Rubus crataegifolius fruits; 4) washing the separated Rubus crataegifolius seeds in running water for 10 minutes; 5) hot air drying the washed Rubus crataegifolius seeds at 60°C for 30 minutes; and 6) pulverizing the dried Rubus crataegifolius seeds into 70 to 1000 μm by using a bowl mill pulverizer.

Description

The method for extracting fruit of wild strawberry and Cosmetic composition containing its seed}

The present invention relates to a massage scrub agent capable of removing dead skin cells and removing impurities in the pores and supplementing nutrients to the skin. The present invention relates to an extraction process for separating raspberry extract and raspberry seeds, and a raspberry extract and seeds separated. The invention relates to a body scrub cosmetic composition. In general, skin is classified into the epidermis, dermis, and subcutaneous fat layer. Among them, the epidermis is further subdivided into the stratum corneum, granule layer, play layer, and basal layer, and the epidermal cells of the basal layer differentiate while rising to the top of the skin, and finally reach the stratum corneum. Epidermal cells that reach the stratum corneum lose their nuclei and are converted into dead cells as they are filled with an insoluble protein called keratin, and the stratum corneum is composed of differentiated epidermal cells and skin lipids that fill between them. It protects the body from external physical, chemical, and biological stimuli and functions as a defense. If the stratum corneum is not removed normally and remains on the skin surface, the stratum corneum becomes thicker and the skin becomes rough, and impurities remaining on the skin surface or hair follicles are oxidized or decomposed by oxygen or microorganisms, causing skin trouble. Various exfoliating scrub agents to protect the skin from skin cells are being studied. The present invention relates to a cleansing cosmetic composition capable of improving skin health with a raspberry body scrub containing raspberry seeds and at the same time expecting a skin exfoliating effect.

Cosmetic compositions such as whitening, cleansing, and cleansing refer to cosmetics that are responsible for removing contaminants from the skin in order to clean the skin. Skin pollutants are divided into exogenous pollutants (various chemical substances in the air, dust, bacteria, cosmetics used for the skin, etc.) and endogenous pollutants (sebum, sebum deterioration, various excreta contained in sweat, aging dead skin cells, various waste products, etc.) In particular, contaminants such as fine dust adhere to the skin and play a role in breeding various bacteria, which adversely affects skin health. Cleansing cosmetic compositions that can easily remove such skin contaminants are in the spotlight, but if the cleaning effect is too strong, the skin dead skin cells are severely removed, making the skin rough or even removing all oils, resulting in skin tightening or rough skin. Since phenomena such as losing occur, there is a growing need for the development of a cleansing cosmetic composition that has excellent effects such as exfoliating and has excellent skin protection effects.

"Cosmetic composition for whitening treatment containing bokbunja extract" of Korean Patent Application Publication No. 2002-0044276 relates to a whitening cosmetic composition useful for the treatment of symptoms such as spots and freckles. In order to obtain excellent whitening effect on the skin, bokbunja extract is the main component A composition is disclosed in which a composite herbal extract in which one or more herbal extracts selected from the group consisting of Dok-Hwa, Astragalus, Honghwaja, Gyeji, Cinnamon, Yugeunpi, Uuiyiin and Aloe Vera are optionally mixed with a cosmetically acceptable base .

In addition, Korean Patent Registration No. 10-1081059 discloses a flower mixture extract having antioxidant and whitening activity, an extraction method thereof, and a cosmetic composition containing the flower mixture extract, and Korean Patent Registration No. 10-1736775 Discloses the invention of a cosmetic composition containing bokbunja extract as an active ingredient, and Korean Patent Registration No. 10-1510092 discloses a pharmaceutical composition for skin external use for the prevention and treatment of whitening and skin aging containing bokbunja seed extract as an active ingredient Or the invention related to a cosmetic composition is disclosed. In addition, Korean Patent Registration No. 10-1908408 discloses a cosmetic composition for exfoliation comprising one or more natural powders selected from the group consisting of apricot seed powder, raspberry seed powder, walnut shell powder, volcanic ash powder, and coral powder as an active ingredient. In Korean Patent Application Publication No. 2007-0022949, after mixing 1% to 10% by weight of apricot seed powder, 1% to 10% by weight of red bean flour, 1% to 10% by weight of black soybean flour, Songhwa Powder 1 A scrub composition for massage is disclosed in which a weight% to 10% by weight is added, and Korean Patent Registration No. 10-1717496 contains bamboo water, lipophilic and hydrophilic solvents, scrub beads, moisturizers, surfactants, builders, thickeners and purified water. It is a cleansing cosmetic composition characterized in that it comprises, in addition to a number of prior art has been disclosed.

Accordingly, the present inventors have endeavored to develop a cleansing cosmetic using natural substances that can reduce skin irritation by chemical substances and the like, and that can be safely used for sensitive skin, and then developed a cosmetic for exfoliating keratin using raspberry extract and raspberry seeds.

Republic of Korea Patent Publication No. 2002-0044276 Korean Patent Registration No. 10-1081059 Korean Patent Registration No. 10-1736775 Korean Patent Registration No. 10-1510092 Korean Patent Registration No. 10-1908408 Republic of Korea Patent Publication No. 2007-0022949 Korean Patent Registration No. 10-1717496

n BJ, Lee CE, Son JH, Lee JY, Choi GH and Park TS. (2005) Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rhododendron mucronulatum T. J. Korean Soc. Appl. Biol. Chem. 48(3), 280-284. Smith, LT, Holbrook, KA, Madri, JA. (1986) Collagen types Ⅰ,Ⅲ, and Ⅴ in human embryonic and fetal skin. Am J Anat. 175(4), 507-521. Colin R, (2000) Goding Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage. Genes & Dev. 14, 1712-1728.

An object of the present invention is to provide a functional cosmetic composition that has less irritation to the skin and effectively removes dead skin cells.

In addition, it is an object of the present invention to extract an extract containing ferulic acid, myricetin, quercetin, and campferol, which are polyphenols, from red and black fruits, which are raspberries. There is a purpose.

In addition, an object of the present invention is to separate the seed portion used for exfoliation from raspberry fruit.

In addition, the present invention is to provide a cosmetic composition for exfoliating keratin containing the extract and raspberry seeds, and as an example, raspberries (scientific name Rubus crataegifolius Bunge), which is most commonly grown in Korea, were used.

In order to achieve the above object, the process of extracting the cosmetic extract from the berries of raspberries

① Process of washing 10 kg of raspberry fruit in running water for 10 minutes;

② after washing is complete, adding 35L of ethanol and 15L of purified water, stirring at 50°C for 5 hours to extract;

③ The extract is separated using a 20 μm sieve and then freeze-dried to powder it:;

The raspberry seed separation process

① Process of washing 10 kg of raspberry fruit in running water for 10 minutes;

② after washing is complete, adding 35L of ethanol and 15L of purified water, stirring at 50°C for 5 hours to extract;

③ the process of separating the extract and seeds of raspberry fruit;

④ Washing the separated raspberry seeds in running water for 10 minutes;

⑤ Process of hot air drying the washed raspberry seeds at 60°C for 30 minutes;

⑥ Process of pulverizing dried raspberry seeds into 70-1000μm) using a ball mill grinder;

The body scrub cosmetic composition containing the raspberry extract and seeds according to the present invention has a significantly higher anti-wrinkle activity function compared to the general anti-wrinkle activity function exhibited by the natural product extract, and is very much compared to the whitening activity function of the general natural product extract. It has been confirmed that it has a high whitening activity function, and excellent exfoliation effect is remarkably improved while minimizing skin irritation, side effects, or skin irritation even when removing dead skin cells, and it provides skin elasticity and anti-wrinkle function to smooth the skin. , It has been found to have antioxidant, whitening, moisturizing, anti-inflammatory, antibacterial, and aging delay effects on the skin.

1 is a process chart of extraction of raspberry fruit extract and separation of raspberry seed scrub.

Terms used in the present specification have selected general terms that are currently widely used as possible while considering functions in the present invention, but this may vary depending on the intention or precedent of a technician working in the field, the emergence of new technologies, and the like. In addition, in certain cases, there may be terms arbitrarily selected by the applicant, but the meaning should be grasped in consideration of the meaning described or used in the detailed description of the invention, not the name of a simple term. And, terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless explicitly defined in the present application, in an ideal or excessively formal meaning. It is not interpreted.

In the present invention, the raspberry fruits of red and black fruits are collected, washed, stirred in 70% ethanol, washed with a 20 μm sieve, washed with hot air, dried and pulverized to be used as a raw material for exfoliation cosmetic The extract, which was stirred in 70% ethanol and filtered through a 20 μm sieve, was concentrated with a vacuum concentrator and freeze-dried at -70° C. to powder, and used as a raw material for whitening and wrinkle-improving cosmetics.

Rubus crataegifolius Bunge (Rubus crataegifolius Bunge) is native to Korea and China and belongs to the reed strawberry tree botanically. Pink flowers bloom in May-June, fruit reddens in July-August, and later turn black. It is mostly wild in the southern part of our country. Raspberries are known to contain organic sugars and sugars and a small amount of vitamin C, and the taste is sweet and sour, and the weakness is warm and non-toxic. It mainly acts on the kidneys, so it has an effect of enhancing virility, and is effective in symptoms of oil wells, dreams, and frequent urine.

In addition, ferulic acid, a kind of polyphenols contained in raspberries, myricetin, quercetin, and kaempferol are contained, and ferulic acid is a cell of the skin. The main component of the external substrate is a representative phenolic compound that increases the production of hyaluronic acid. It has the function of improving the function of improving dryness, wrinkles, and loss of elasticity caused by a decrease in hyaluronic acid, and myricetin ) Has an excellent moisturizing effect and has excellent skin regeneration effect and skin elasticity improvement effect by promoting collagen production in damaged skin tissues, quercetin has excellent whitening effect, and campferol is an antioxidant. , Antibacterial and anti-aging effects, and raspberry seeds have significantly improved the excellent exfoliation effect while minimizing skin irritation, side effects or skin irritation in whitening, wrinkle improvement, and exfoliation of the skin. However, it has been shown to be effective in anti-wrinkle, anti-aging, and skin seizure removal.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

1-1. Extraction process of raspberry fruit

① Process of washing 10 kg of raspberry fruit under running water for 10 minutes.

② After washing is complete, 35L of ethanol and 15L of purified water are added, followed by stirring at 50°C for 5 hours to extract.

③ A process of separating the extract using a 20μm sieve and then lyophilizing it to powder.

Plants of the genus Raspberry used for extraction include R. parvifolius L. f. parvifolius, R. oldhamii Miq., R. coreanus Miq., and R. crataegifolius Bunge. , Bear strawberry (R. phoenicolasius Maxim. f. phoenicolasius).

1-2. Raspberry seeds Scrub Separation process

① Process of washing 10 kg of raspberry fruit under running water for 10 minutes.

② After washing is complete, 35L of ethanol and 15L of purified water are added, followed by stirring at 50°C for 5 hours to extract.

③ The process of separating the extract and seeds of raspberry fruit.

④ The process of washing separated raspberry seeds in running water for 10 minutes.

⑤ Process of drying the washed raspberry seeds with hot air at 60℃ for 30 minutes.

⑥ Process of grinding dried raspberries seeds into 70-1000μm) using a ball mill.

Plants of the genus Raspberry for use with seeds are R. parvifolius L. f. parvifolius, R. oldhamii Miq., R. coreanus Miq., and R. crataegifolius Bunge. ), bear strawberry (R. phoenicolasius Maxim. f. phoenicolasius).

1-3. raspberry body Scrub Composition of composition and composition ratio

<Composition A> Ingredient classification Composition Composition ratio (parts by weight) Remark A-1 Distilled water 100 A-2 Disodium EDTA (EDTA-2Na) 0.2 A-3 Acrylates Copolymer 10

<Composition B> Ingredient classification Composition Composition ratio (parts by weight) Remark B-1 Sodium Lauryl Ether Sulfate 100 B-2 Potassium Hydroxide 1.2 B-3 Glycerin 14 B-4 Xanthan gum One B-5 Distilled water 12 B-6 Cocamidopropyl betaine 40 B-7 Cocamide DEA 20 B-8 Methylparaben (methylparaben) One B-9 Propyl paraben 2

<Composition C> Ingredient classification Composition Composition ratio Remark C-1 Distilled water 100 C-2 Citric acid 10

<Composition D> Ingredient classification Composition Composition ratio (parts by weight) Remark D-1 Raspberry Seed Scrub 50 D-2 Apricot seed scrub 100 D-3 Raspberry extract 75 D-4 Oil, blueberry, mushroom, gooseberry, centella asiatica complex extract 50 Same content

<Composition E> Ingredient classification Composition Composition ratio Remark E-1 Phenoxyethanol 60 E-2 Fragrance 100

The raspberry body scrub composition of the present invention is composed of the components of <Table 1> to <Table 5>.

After mixing each component in the ratio of <Table 1> to <Table 5>, composition A to composition E were mixed in the ratio of <Table 6> to prepare a raspberry body scrub composition.

<The composition ratio of the raspberry body scrub composition> Composition Composition ratio (parts by weight) Composition A 80-100 Composition B 100 Composition C 3-5 Composition D 2-4 Composition E 0.5-1

2-1. Mixed with raspberry red/black Hydrothermal Cytotoxicity of the extract ( MTT ) evaluation

For the skin cell line whose stability was confirmed, the long-term raspberry hot water extract was treated by concentration, and then the degree of cell death was measured through a cytotoxicity test (MTT assay) to confirm toxicity. The cytotoxicity test (MTT assay) uses the principle that the original yellow MTT formazan is reduced in the mitochondria of living cells and changes its color to purple. Since the amount of purple color decreases according to the degree of cell death due to cytotoxicity, toxicity can be easily evaluated through absorbance measurement. Through the cytotoxicity evaluation, when long-term raspberry hot water extract 1 ~ 200ppm was treated, a cell viability of 95% or more was confirmed, and when the extract was treated at a concentration of 400ppm, about 17% of cell death was confirmed as shown in <Figure 1>. The raspberry extract contained in the composition of the raspberry body scrub composition of the present invention was extracted at a concentration of 200 ppm.

<Figure 1>

2-2. Raspberry fruit (red fruit/black fruit) Hydrothermal Extract of Whitening function Efficacy evaluation

Through a tyrosinase activity inhibition test, it was confirmed that the whitening activity increased in a concentration-dependent manner at a concentration of 1 to 200 ppm when the long-term raspberry hot water extract 1 to 400 ppm was treated, and about 21% whitening activity when the extract was treated at a concentration of 400 ppm It was confirmed that it represents. Compared to the whitening activity of Vitamin C used as a positive control, it has about 30% whitening effect, and since the whitening activity of general natural extracts reaches about 10 to 20%, compared to the general whitening activity function of natural extracts. It was confirmed as shown in <Figure 2> that it has a rather high whitening activity function.

<Figure 2>

2-3. Raspberry fruit (red fruit/black fruit) Hydrothermal Evaluation of anti-wrinkle functional efficacy of extract

Through the PPE (Porcine Pancreatic Elastase) Assay test, it was confirmed that when long-term raspberry hot water extract 1 to 400 ppm was treated, the wrinkle improvement activity increased in a concentration-dependent manner at a concentration of 1 to 400 ppm, and about 52% when the extract was treated at a concentration of 400 ppm. It was confirmed that it showed wrinkle improvement activity. Compared to the anti-wrinkle activity of ursolic acid used as a positive control, it has about 60% anti-wrinkle effect, and it has a significantly higher anti-wrinkle activity function compared to the general anti-wrinkle activity function shown in natural extracts <Figure 3> It was confirmed as follows.

<Figure 3>

Claims (3)

① Process of washing 10 kg of raspberry fruit in running water for 10 minutes;
② After washing is complete, adding 35L of ethanol and 15L of purified water, stirring at 50°C for 5 hours to extract;
③ The process of separating the raspberry fruit extract and seeds using a 20μm sieve;
④ The process of concentrating the separated raspberry fruit extract at 60℃, -0.006MPa in a gamam concentrator;
⑤ process of lyophilizing the concentrated extract at -70℃ and then powdering it;
Method for extracting a raspberry fruit extract, characterized in that obtained from. ① Process of washing 10 kg of raspberry fruit in running water for 10 minutes;
② After washing is complete, adding 35L of ethanol and 15L of purified water, stirring at 50°C for 5 hours to extract;
③ The process of separating the extract and seeds of raspberry fruit using a 20μm sieve;
④ Washing the separated raspberry seeds in running water for 10 minutes;
⑤ Process of hot air drying the washed raspberry seeds at 60°C for 30 minutes;
⑥ Process of grinding dried raspberry seeds into 70-1000 μm) using a ball mill grinder; a method of separating and extracting raspberry seed scrub, characterized in that obtained from. A body scrub composition of a plant of the genus Raspberry, characterized in that the components consisting of the following <Composition A> to <Composition E> are mixed in the same ratio as in <Composition F>.

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