KR102310943B1 - The method for extracting fruit of wild strawberry and Cosmetic composition containing its seed - Google Patents

Abstract

The present invention relates to a cosmetic raspberry body scrub composition comprising extracts and seeds separated from raspberry fruits, and the process of extracting a cosmetic extract from raspberry fruits is
① The process of washing 10 kg of raspberry fruit under running water for 10 minutes;
② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours;
③ The process of separating the extract using a 20 μm sieve and then freeze-drying it to powder;
The process of separating raspberry seeds from the fruits of the genus raspberry is
① The process of washing 10 kg of raspberry fruit under running water for 10 minutes;
② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours;
③ The process of separating the extract and seeds of the fruit of the genus Raspberries;
④ Washing the separated raspberry seeds in running water for 10 minutes;
⑤ The process of drying the washed raspberry seeds with hot air at 60°C for 30 minutes;
⑥ The process of pulverizing dried raspberry seeds to 70-1000 μm) using a ball mill grinder;
To prepare a body scrap cosmetic composition containing these powdered raspberry extracts and raspberry seeds.

Description

Method for producing a body scrap cosmetic composition containing raspberry extract powder and raspberry seed powder {The method for extracting fruit of wild strawberry and Cosmetic composition containing its seed}

The present invention relates to a massage scrub that can remove dead skin cells from the skin and remove wastes in the pores while replenishing nutrients to the skin. The present invention relates to a method for preparing a body scrub cosmetic composition. In general, the skin is classified into the epidermis, dermis, and subcutaneous fat layer. Of these, the epidermis is further subdivided into the stratum corneum, granular layer, stratum corneum, and basal layer, and the epidermal cells of the basal layer are differentiated as they ascend to the top of the skin, and finally reach the stratum corneum. The epidermal cells that reach the stratum corneum lose their nuclei and are filled with an insoluble protein called keratin and are converted into dead cells. It prevents going outside and protects the human body from external physical, chemical, and biological stimuli. When the stratum corneum is not exfoliated normally and remains on the skin surface, the stratum corneum thickens and the skin becomes rough. Also, impurities remaining on the skin surface or hair follicles are oxidized or decomposed by oxygen or microorganisms, causing skin troubles. Various exfoliating scrubs are being researched to protect the skin from acne. The present invention relates to a cleansing cosmetic composition that can improve skin health with a raspberry genus body scrub containing raspberry seeds and at the same time can expect a skin exfoliation effect, etc.

Cosmetic compositions such as whitening, cleaning, and cleansing refer to cosmetics that have a function of removing skin contaminants to keep the skin clean. Skin contamination sources are divided into extrinsic pollutants (various chemicals in the air, dust, bacteria, cosmetics, etc.) and intrinsic pollutants (sebum, sebum altered substances, various excrements contained in sweat, aged keratin, various wastes, etc.) In particular, pollutants caused by fine dust, etc. attach to the skin and reproduce various bacteria, thereby adversely affecting skin health. A cosmetic composition for cleaning that can easily remove these skin contaminants is in the spotlight, but if the cleaning effect is too strong, the dead skin cells are severely removed, making the skin rough or removing all the oil, causing the skin to be pulled or rough. Since the phenomenon such as loss of skin appears, there is a growing need for the development of a cleansing cosmetic composition that is excellent in the effect of exfoliating the skin while also having an excellent skin protection effect.

Korean Patent Application Laid-Open No. 2002-0044276, "Cosmetic composition for whitening treatment comprising extract of bokbunja" relates to a cosmetic composition for whitening useful for the treatment of symptoms such as spots and freckles. Disclosed is a composition in which a complex herbal extract optionally blended with one or more herbal extracts selected from the group consisting of poison ivy, astragalus, safflower, cinnamon, cinnamon, Eugeunpi, Uiin, and aloe vera is mixed with a cosmetically acceptable base. .

In addition, Korean Patent No. 10-1081059 discloses an invention related to a flower mixed extract having antioxidant and whitening activity, a method for extracting the same, and a cosmetic composition containing the flower mixed extract, and Korean Patent No. 10-1736775 discloses the invention of a cosmetic composition containing bokbunja extract as an active ingredient, and Korean Patent No. 10-1510092 discloses a pharmaceutical composition for external use for skin whitening and prevention and treatment of skin aging containing bokbunja seed extract as an active ingredient Or the invention relates to a cosmetic composition is disclosed. And, Korean Patent Registration No. 10-1908408 discloses a cosmetic composition for exfoliating dead skin cells comprising, as an active ingredient, at least one natural powder selected from the group consisting of apricot seed powder, raspberry seed powder, walnut shell powder, volcanic ash powder and coral powder. In Korean Patent Application Laid-Open No. 2007-0022949, after mixing 1% to 10% by weight of apricot seed powder, 1% to 10% by weight of red bean powder, and 1% to 10% by weight of black bean powder, pine flower powder 1 A scrub composition for massage in which weight% to 10% by weight is added is disclosed, and Republic of Korea Patent No. 10-1717496 discloses bamboo water, lipophilic and hydrophilic solvents, scrub beads, moisturizing agents, surfactants, builders, thickeners and purified water. There is a cleansing cosmetic composition comprising, in addition, a number of prior art is disclosed.

Accordingly, the present inventors have developed a cosmetic for exfoliating dead skin cells using raspberry extract and raspberry seed after efforts to reduce skin irritation caused by chemicals and to develop a cleansing cosmetic using natural substances that can be safely used even on sensitive skin.

Republic of Korea Patent Publication No. 2002-0044276 Republic of Korea Patent No. 10-1081059 Republic of Korea Patent No. 10-1736775 Republic of Korea Patent No. 10-1510092 Republic of Korea Patent Registration No. 10-1908408 Republic of Korea Patent Publication No. 2007-0022949 Republic of Korea Patent No. 10-1717496

n BJ, Lee CE, Son JH, Lee JY, Choi GH and Park TS. (2005) Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rhododendron mucronulatum T. J. Korean Soc. Appl. Biol. Chem. 48(3), 280-284. Smith, LT, Holbrook, KA, Madri, JA. (1986) Collagen types Ⅰ, Ⅲ, and V in human embryonic and fetal skin. Am J Anat. 175(4), 507-521. Colin R, (2000) Goding Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage. Genes & Dev. 14, 1712-1728.

An object of the present invention is to provide a functional cosmetic composition that has little irritation to the skin and effectively removes dead skin cells.

In addition, it is an object of the present invention to extract an extract containing ferulic acid and myricetin, quercetin and kaempferol, which are a kind of polyphenols, from red and black fruits, which are wild strawberries. There is a purpose.

Another object of the present invention is to separate the seed part used for exfoliating the keratin from the fruit of the genus Wild strawberry.

In addition, the present invention provides a cosmetic composition for exfoliating keratin containing the extract and wild strawberry seeds, and as an example, wild strawberries (scientific name: Rubus crataegifolius Bunge) cultivated the most in Korea were used.

In order to achieve the above object, the process of extracting the cosmetic extract from the fruit of the genus Raspberries is

① The process of washing 10 kg of raspberry fruit under running water for 10 minutes;

② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours;

③ The process of separating the extract using a 20 μm sieve and then freeze-drying to powder:

Strawberry seed separation process

① The process of washing 10 kg of raspberry fruit under running water for 10 minutes;

② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours;

③ The process of separating the extract and seeds of the fruit of the genus Raspberries;

④ Washing the separated raspberry seeds under running water for 10 minutes;

⑤ The process of drying the washed raspberry seeds in hot air at 60°C for 30 minutes;

⑥ Process of pulverizing dried raspberry seeds into 70-1000 μm using a ball mill grinder; and the present invention is a cosmetic composition for body scrub containing raspberry extract powder and raspberry genus seed powder produced by these processes.

The cosmetic composition for body scrub containing raspberry extract and seeds according to the present invention has a significantly higher wrinkle-improving activity compared to the general anti-wrinkle activity shown in natural extracts, and also compared to the whitening activity of common natural extracts. It was confirmed that it has a very high whitening activity, and the excellent keratin exfoliation effect was remarkably improved while minimizing skin irritation, side effects, or skin irritation in removing dead skin cells. It has been confirmed to have antioxidant, whitening, moisturizing, anti-inflammatory, antibacterial and aging effects on the skin.

1 is a process diagram of extracting the fruit extract of the genus raspberry and separation of the raspberry seed scrub.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there may be a term arbitrarily selected by the applicant, but the meaning should be grasped in consideration of the meaning described or used in the detailed description of the invention rather than the name of the simple term. In addition, terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, in an ideal or excessively formal sense not interpreted

The present invention collects raspberry fruits of red and black fruits, washes them, stirs them in 70% ethanol, separates and washes raspberry seeds using a 20 μm sieve, washes them with hot air, and then pulverizes them as raw materials for exfoliating keratin In the above, the extract, stirred in 70% ethanol and filtered using a 20 μm sieve, is concentrated with a reduced pressure concentrator and freeze-dried again at -70° C. .

The wild strawberry (Rubus crataegifolius Bunge) is native to Korea and China and belongs to the raspberry family botanically. Pink flowers bloom in May-June, and red fruits in July-August, which later turn black. It is mostly wild in the southern regions of our country. Raspberries are known to contain organic sugars and sugars and a small amount of vitamin C. The taste is sweet and sour, and the weakness is warm and non-toxic. Because it mainly acts on the kidneys, it has an energy-enhancing effect and is effective for symptoms such as oily, dreamy, and frequent urination.

In addition, ferulic acid, myricetin, quercetin, and kaempferol, which are a kind of polyphenol contained in raspberries, are contained, and ferulic acid is the cell of the skin. The main component constituting the external matrix is a representative phenolic compound that increases the production of hyaluronic acid. ) has been shown to have an excellent skin regeneration effect and skin elasticity improvement effect by promoting excellent moisturizing effect and collagen production in damaged skin tissue, quercetin has an excellent whitening effect, and kaempferol is an antioxidant , antibacterial and anti-aging effects, and raspberry seeds have a whitening function, wrinkle improvement, and removal of dead skin cells, while minimizing skin irritation, side effects, and skin irritation while remarkably improving the excellent exfoliating effect, and skin elasticity It has been shown to be effective in wrinkle suppression, anti-aging and removal of skin texture.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

1-1. Extraction process of raspberry fruit

① The process of washing 10 kg of raspberry fruit under running water for 10 minutes.

② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours.

③ The extract is separated using a 20 μm sieve, and then freeze-dried and powdered. .), raspberry (R. coreanus Miq.), raspberry (R. crataegifolius Bunge), and raspberry (R. phoenicolasius Maxim. f. phoenicolasius) were limited.

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1-2. Separation process of raspberry seed scrub

① The process of washing 10 kg of raspberry fruit under running water for 10 minutes.

② After washing is complete, 35L of ethanol and 15L of purified water are added, and the extraction is performed by stirring at 50°C for 5 hours.

③ The process of separating the extract and seeds of the fruit of the genus Raspberries.

④ The process of washing the separated raspberry seeds under running water for 10 minutes.

⑤ The process of drying the washed raspberry seeds in hot air at 60℃ for 30 minutes.

⑥ The process of pulverizing dried raspberry seeds into 70-1000 μm using a ball mill grinder.

Raspberries for use with seeds are R. parvifolius L. f. parvifolius, R. oldhamii Miq., Japanese raspberry (R. coreanus Miq.), R. crataegifolius Bunge ), and strawberry (R. phoenicolasius Maxim. f. phoenicolasius).

1-3. raspberry body scrub Composition of the composition and composition ratio

<Composition A> Ingredient classification composition Composition ratio (parts by weight) note A-1 Distilled water 100 A-2 Disodium EDTA (EDTA-2Na) 0.2 A-3 Acrylates Copolymer 10

<Composition B> Ingredient classification composition Composition ratio (parts by weight) note B-1 Sodium Lauryl Ether Sulfate 100 B-2 Potassium Hydroxide 1.2 B-3 Glycerin 14 B-4 Xanthan gum One B-5 Distilled water 12 B-6 Cocamidopropyl betaine 40 B-7 Cocamide DEA 20 B-8 Methylparaben (methylparaben) One B-9 Propyl paraben 2

<Composition C> Ingredient classification composition composition ratio note C-1 Distilled water 100 C-2 Citric acid 10

<Composition D> Ingredient classification composition Composition ratio (parts by weight) note D-1 Raspberry Seed Scrub 50 D-2 Apricot Seed Scrub 100 D-3 raspberry extract 75 D-4 Oil, Blueberry, Sanghwang mushroom, Spiny hornwort, Centella asiatica complex extract 50 same content

<Composition E> Ingredient classification composition composition ratio note E-1 Phenoxyethanol 60 E-2 Fragrance 100

The Raspberries body scrub composition of the present invention is composed of the ingredients of <Table 1> to <Table 5>.

After mixing each component in the ratios of <Table 1> to <Table 5>, compositions A to E were combined in the ratios of <Table 6> to prepare a raspberry body scrub composition.

<Composition ratio of raspberry body scrub composition> composition Composition ratio (parts by weight) composition A 80-100 composition B 100 composition C 3 - 5 composition D 2 - 4 composition E 0.5 - 1

2-1. Mixed with raspberry red/black hot water The cytotoxicity of the extract ( MTT ) evaluation

For skin cell lines whose stability was confirmed, the hot water extract of long-term wild raspberry was treated by concentration, and the degree of cell death was measured through a cytotoxicity test (MTT assay) to confirm toxicity. The cytotoxicity test (MTT assay) uses the principle that MTT formazan, which is originally yellow, is reduced in the mitochondria of living cells and changes its color to purple. Because the amount of purple color decreases according to the degree of cell death due to cytotoxicity, toxicity can be easily evaluated through absorbance measurement. Through cytotoxicity evaluation, cell survival rate of more than 95% was confirmed when 1 ~ 200ppm of long-term raspberry hot water extract was treated, and about 17% of cell death was confirmed when the extract was treated at 400ppm concentration as shown in <Figure 1>. The raspberry extract included as a component in the raspberry body scrub composition of the present invention was extracted at a concentration of 200 ppm.

<Figure 1>

2-2. Efficacy evaluation of whitening functionalities of hot water extracts from wild strawberry fruit (red fruit/black fruit)

Through the tyrosinase activity inhibition test, it was confirmed that the whitening activity increased in a concentration-dependent manner at the concentration of 1 ~ 200ppm when 1 ~ 400ppm of long-term wild strawberry hot water extract was treated. It was confirmed that the Compared to the whitening activity of Vitamin C used as a positive control, it has a whitening effect of about 30%, and the whitening activity of a general natural extract reaches 10 to 20%, so compared to the general whitening activity shown by natural extracts, It was confirmed that it has a rather high whitening activity as shown in <Figure 2>.

<Figure 2>

2-3. Raspberry fruit (red fruit/black fruit) hot water Wrinkle improvement functional efficacy evaluation of extracts

Through the PPE (Porcine Pancreatic Elastase) Assay test, it was confirmed that the wrinkle improvement activity increased in a concentration-dependent manner at the concentration of 1 to 400 ppm when the long-term wild strawberry hot water extract was treated with 1 ~ 400 ppm, and when the extract was treated with the concentration of 400 ppm, about 52% It was confirmed that it exhibits wrinkle improvement activity. Compared to the wrinkle-improving activity of Ursolic acid used as a positive control, it has an anti-wrinkle effect of about 60% and has a significantly higher wrinkle-improving activity compared to the general wrinkle-improving activity shown in natural extracts <Figure 3> was confirmed as

<Figure 3>

Claims (3)

After washing 10 kg of Raspberries in running water for 10 minutes, add 35L of ethanol and 15L of purified water, stir at 50°C for 5 hours to extract, and then use a 20μm sieve to separate the extracts and seeds of Raspberries.
The process of lyophilizing the extract obtained by concentrating the separated fruit extract of the genus raspberry with a vacuum concentrator at -70° C. and then powdering it.
The process of washing the separated raspberry seeds in running water for 10 minutes, drying them with hot air at 60° C. for 30 minutes, and pulverizing them to 70-1000 μm using a ball mill grinder;
Method for producing a body scrub cosmetic composition comprising the powdered raspberry fruit and powdered raspberry seed consisting of
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