KR101710005B1 - Cosmetic composition comprising cheonjeongpalgindan as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition, which comprises cheonjeongpalgindan (eight kinds of medicinal herbs) as active ingredients, the cheonjeongpalgindan accounting for 0.0001-30.0 wt% with respect to a total weight of the cosmetic composition, which comprises the cheonjeongpalgindan as the active ingredients. According to the present invention, the cheonjeongpalgindan prevents skin aging, and specifically, has effects of aquaporin biosynthesis promotion, filaggrin expression promotion, inhibition of melanin generation, hindrance of elastin decomposition enzyme, anti-oxidation, collagen biosynthesis improvement, moisturization, whitening, skin wrinkle enhancement, skin elasticity enhancement, enhancement of inner skin density, thereby providing various functional cosmetics.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing a cephalothorax diagnosis as an active ingredient,

The present invention relates to a cosmetic composition comprising a cinnabar underarm diagnosis, specifically to a cosmetic composition comprising an extract of eight herbal medicines.

A variety of physical and chemical changes occur in human skin during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by active metabolism, Can quickly recover and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to control the amount and activity of MMP expression induced by intracellular natural aging and photoaging.

In order to solve these various problems, recently, many cosmetics using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

Therefore, the present inventors selected eight herbal medicines as a result of studies on applicability of cosmetics to various natural products not yet known. 8 kinds of herb medicines were Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong, Veterinary Medicine and Rhizoma. The present invention relates to a method for producing an extract from a quartz arm diagnosis, comprising the steps of: (a) accelerating aquaporin biosynthesis promotion, filagrin expression promoting effect, melanin formation inhibitory effect, elastin degrading enzyme inhibitory effect, antioxidative effect, collagen biosynthesis improving effect, Skin wrinkle improvement effect, skin elasticity improvement effect, dermal density improvement effect and skin antiaging effect were measured. As a result, it was found that the efficacy can be expected as a cosmetic product because of its excellent efficacy.

It is an object of the present invention to provide a method for improving aquaporin biosynthesis, a method for promoting aqua purine biosynthesis, a method for promoting fila green expression, an effect for inhibiting melanin production, an inhibitor for elastin degrading enzyme, an antioxidant effect, an effect for enhancing collagen biosynthesis, a moisturizing effect, A skin elasticity improvement effect, a dermal density improvement effect, and a skin antiaging effect.

The present invention is achieved by a cosmetic composition comprising as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong, Veterinary Medicine and Rhizoma.

Preferably, the cosmetic composition has an antioxidative effect.

Preferably, the cosmetic composition has skin moisturizing effect.

Preferably, the cosmetic composition has an effect of improving skin elasticity.

Preferably, the cosmetic composition has an effect of improving skin wrinkles.

Preferably, the cosmetic composition has a skin whitening effect.

Preferably, the cosmetic composition has an effect of improving the dermal density of the skin.

Preferably, the herbal medicine extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the medicinal herb extract is selected from the group consisting of (a) water, anhydrous or lowered alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, Hexane, and a mixture thereof, or (b) an extraction method using fermentation or natural fermentation from a microorganism.

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray Lt; RTI ID = 0.0 > of: < / RTI >

The results of the present invention are summarized as follows. The diagnosis of the cephalic arm according to the present invention is made on the basis of the effect of accelerating the biosynthesis of aquaporin (Experimental Example 1), the promotion of pilar green expression (Experimental Example 2), the melanin formation inhibitory effect (Experimental Example 3), the elastin degrading enzyme inhibitory effect (Experimental Example 5), skin whitening effect (Experimental Example 5), collagen biosynthesis effect (Experimental Example 7), moisturizing effect (Experimental Example 8), whitening effect (Experimental Example 9) (Experimental Example 10), skin elasticity improving effect (Experimental Example 11), and dermal density improving effect (Experimental Example 12).

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference. The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

As used in the present invention, the term " diagnosis of the heavenly shell "means eight kinds of medicinal herbs ingredients such as Gujia, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,

Ginseng is a perennial herbaceous plant belonging to Araliaceae, and its scientific name is Panax ginseng . It is often grown as a plant growing in deep mountain regions. Its height reaches 60㎝, rhizomes are short, stand straight or diagonally, and bottom like bellflower develops. It is widely cultivated in Korea and China. Ginseng is used for body weakness, boredom, fatigue, loss of appetite, vomiting and diarrhea. It helps the lung function and increases the reflex activity and new function. Pharmacological actions have been reported in cerebral cortex excitement and inhibition, anti-fatigue, immune enhancement, cardiac contraction, hyperglycemia inhibition, promoting protein synthesis, homeostasis, anticancer, and detoxification. Also, it has been reported that ginsenoside F5 of ginseng leaves has a whitening and anti-inflammatory effect, and that ginseng fruit inhibits melanin synthesis and has a skin whitening effect.

It is a deciduous broad-leaved shrub with a purple flower bloomed in June-September, and the fruit is round or elliptical, and ripens red in August and September. [甘寒] It is dizzy, it has no strength in the back and knees, it is used when the man is fat and can not get pregnant. When the blood is weak, the face is yellow and the hair is whitish, it is effective when it does not sleep. It is also used for long seawater due to fragility. Non-specific immune enhancement, hematopoiesis, cholesterol lowering, anti-hepatic action, blood pressure lowering, blood glucose lowering, growth promotion, and anti-cancer activity have been reported as pharmacological actions.

Licorice is a perennial herbaceous plant belonging to the soybean family and used as herb medicine by drying the roots. Its scientific name is Glycyrrhiza uralensis FISCH. Roots are dried and used as medicinal herbs. Glycyrrhizin with sweetness and glabric acid, sucrose, glucose, liquiritin, lycoricidine and the like are contained. Glycyrrhizin has the effect of detoxifying diphtheria toxin, tetanus toxin, codeine hydrochloride, strychinin acetate, snake venom and puffer fish poison. In addition, glycyrrhizin reduced cholesterol levels in the blood, thus lowering the blood pressure, promoting the secretion of bile, and confirming that the blood had a vigorous and analgesic effect.

Peony is a perennial herbaceous plant belonging to the family Ranunculaceae. The flower is big and it is also called a bamboo. There are a variety of varieties, such as peanuts, herbal medicines, herbal medicines, and medicines. Flowers bloom in May to June, and roots are used as herbal medicine in oriental medicine. The ingredient is a glycoside containing paeoniflorin and alkaloid paeonine, and tannin, resin and benzoic acid. It weakens the contraction force of the smooth muscle of the stomach and uterus of the rat, and acts pharmacologically in the intestinal tract of the rabbit to show the effect of ginkgo. The glycoside component inhibits the central nervous system. In addition, it has antimicrobial action, and it inhibits the development of Staphylococcus aureus, Disease, hemolytic streptococcus, and pneumococcus. The weakness is cold, the taste is bitter and bitter. It is effective when there is abdominal pain, diarrhea and abdominal pain due to gastroenteritis and gastrointestinal spastic pain, and it is effective even when there is abdominal pain after abdominal pain and diarrhea. It shows the efficacy of blood, pain, and blood transfusion in the menstrual irregularity and uterine bleeding. It is also used for chronic hepatitis and is also used for the pain of the liver.

It is a perennial plant of the dicotyledonous plant, the monocotyledonous plant, and the medicinal plant. It is a Chinese origin and is cultivated as a medicinal plant. The roots are thick and fleshy, with reddish brown and sideways. In one room, raw thing of roots is raw persimmon, dried persimmon leaves, steamed persimmon leaves are called squid poison. Sukjihu is used as a blood-donor, and it is used for physiological impoverishment, fragile constitution, child's developmental dementia, dementia, premature ejaculation, erectile dysfunction, and raw persimmon is used for fragile constitution, hemorrhage, nosebleed, uterine bleeding, menstrual impurity, constipation, It is effective in thirst and throat caused by heat inside the organ, and it stops the blood and nosebleeds.

Hwanggi is a perennial plant of rosemary beans and grows in the rocks of the mountain. It is 40 ~ 70cm high and has soft white hairs throughout. The stem is a total bud (crow), and the leaves are odd numbered iguate compound leaves consisting of 6 to 11 pairs of small leaves. The small leaf is about 1 ~ 2 cm long and is egg-shaped elliptical, and leaf edge is plain. They are distributed in Korea, Japan, Manchuria, Northeastern China, and Eastern Siberia. Generally, it is cultivated as a herb, and it is collected in autumn in a herb, and it is called "Hwanggi" of herbal medicines and dried in the sun, It is prescribed for the weakness of the body, boredom of fatigue, devitalization, elimination, abstinence, internal sewage, sweating, peripheral nerve disorder.

Hwangjeong is the dried rootstock of the stratum orientalis, a perennial herbaceous lily belonging to the Lily family. Layered roundworms are long oval with slanting leaves. Fruits are round and ripen in black purple. The underground stem grows to the side, and the stem has 6 hairs. The leaves are oval and staggered. The roots were used as medicinal materials, and in the spring and winter, they were used as nursery plants. The roots are used for the treatment of limb weakness, weakness in the spleen, pulmonary tuberculosis, and for the purpose of interpolating, naming and insecting.

Omija is the fruit of the Japanese oak tree and is a dark red color with a diameter of about 1cm. It is a ball shaped with a diameter of about 1cm and a dark red color. It contains 1-2 drops of red juice and reddish brown seeds. It has five flavors: sweet, sour, bitter, salty, and spicy. It contains ingredients such as thiazide, glutamic acid, citrulline, malic acid, and citric acid, which strengthens the heart, lowers the blood pressure, improves immunity, and is used as a tonic. It strengthens the pulmonary function, and it has the function of chinhae and geodam, and it helps to cure cough and thirst.

In the present invention, the diagnosis of the quartz arm may be made using a conventional solvent according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure, preferably (a) water, Propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane (such as methanol, ethanol, , Chloroform, hexane, and mixtures thereof. ≪ / RTI >

In the present invention, a solvent extraction method using an extraction solvent is preferably used. Preferably ethanol, 70% (v / v) ethanol or water, and most preferably 70% (v / v) ethanol.

It is obvious to those skilled in the art that the extract of the present invention having substantially the same effect can be obtained by using the above-described extraction solvent as well as other extraction solvents.

The diagnosis of the cephalic arm according to the present invention includes an extract obtained through a fermentation process. The fermented extract of the diagnosis of the cephalic palate can be prepared as follows. The diagnosis of the cephalic palm is finely crushed to about 100 to 500 mesh, then 1 to 50 g / L of a conventional microbial culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the above-described diagnosis of the cephalic arm is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition, more preferably, 0.01 to 10% by weight of the total amount of the above- do. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant, It is not even.

Therefore, the cosmetic composition of the present invention including the various functions of the present invention for the diagnosis of the quartz arm is for preventing skin aging. Specifically, the anti-aging effect of the skin is excellent in the effect of promoting the aquaporin biosynthesis, the effect of promoting the expression of pilar green, the effect of suppressing melanin formation, the effect of inhibiting elastin degrading enzyme, the antioxidative effect, the effect of collagen biosynthesis, the moisturizing effect, Skin elasticity improvement effect, and dermal density improvement effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One: Diagnosis of a cephalic arm  Preparation of solvent extract

50g of each of 8 kinds of medicinal materials with shade and shade - Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong, Veterinary medicine and Rhizopus were mixed and refluxed for 3 hours with 70% (v / v) , And filtered through a Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 ° C or lower to obtain 87.7 g of a diagnostic solvent extract of a cephalic palate.

Manufacturing example  2 to 3: Diagnosis of a cephalic arm  Preparation of fermented extract

Production Examples 2 to 3 were prepared by fermenting yeast or lactic acid bacteria in Preparation Example 1 as a fermented extract of a diagnosis of a cephalic palate. Lactobacillus MRS broth (DIFCO, USA) was used as a culture medium for lactic acid bacteria. The culture medium for yeast culture was prepared by adding 1 to 30 wt. %, Preferably 10 wt%.

Three saccharide in my process to the YPD medium Levy jiae (Saccharomyces cerevisiae ATCC 18824) was inoculated at 100,000 ~ 10,000,000 cfu and cultured at 30 ~ 37 ° C for 12 ~ 72 hours. After culturing, the culture broth was centrifuged to remove the microorganisms, and ethanol was added to the final 70% (V / V), followed by refluxing three times for 5 hours. After cooling, And filtered through Whatman # 4 filter paper. The filtered fermented extract was filtered with 0.25 μm filter paper, and then concentrated under reduced pressure and freeze-dried at 60 ° C or lower. Ethanol and butyleneglycol were dissolved in at least one solvent so as to contain 0.001 to 70% by weight of the thus obtained reduced-pressure concentrate or lyophilized product to obtain a diagnostic fermentation extract of a quartz arm (Production Example 2).

The Lactobacillus MRS medium was inoculated with Lactobacillus plantarum ATCC 10012 at 100,000 to 10,000,000 cfu and cultured at 30-37 ° C for 12-72 hours. After culturing, the culture broth was centrifuged to remove the microorganisms, and ethanol was added to the final 70% (V / V), followed by refluxing three times for 5 hours. After cooling, And filtered through Whatman # 4 filter paper. The filtered fermented extract was filtered with 0.25 μm filter paper, and then concentrated under reduced pressure and freeze-dried at 60 ° C or lower. Ethanol and butyleneglycol were dissolved in at least one solvent to obtain 0.001 to 70% by weight of the thus obtained reduced-pressure concentrate or lyophilized product.

Comparative Preparation Example 1: Preparation of Gugija extract

After shredding, 50 g of shredded ginger was refluxed with 70% (v / v) aqueous ethanol solution for 5 hours three times, cooled, and filtered through Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure at 50 ° C or lower and lyophilized to obtain 10.3 g of Gugija extract.

Comparative Preparation Example 2: Preparation of ginseng extract

50 g of shredded ginseng was refluxed with 70% (v / v) aqueous ethanol solution for 3 hours, cooled down, and filtered through Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 ° C or lower to obtain 9.2 g of ginseng extract.

Comparative Preparation Example 3: Preparation of Omija extract

50 g of shredded omelet was refluxed for 3 hours with 70% (v / v) aqueous ethanol solution for 5 hours, followed by cooling and filtration with Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower to obtain 8.7 g of Omiza extract.

Comparative Preparation Example 4: Preparation of Hwangjeong Extract

After shredding, 50 g of shaded hull was refluxed with 70% (v / v) aqueous ethanol solution for 5 hours three times, cooled, and filtered through Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 ° C or lower to obtain 9.1 g of Huang Qing extract.

Comparative Manufacturing Example  5: Preparation of Hwanggi extract

50 g of shaded yellow shade was extracted with reflux three times for 5 hours in 70% (v / v) aqueous ethanol solution, cooled down, and filtered through Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower to obtain 8.4 g of the extract of Hwanggi.

Comparative Manufacturing Example  6: Ginger extract  Produce

After shredding, 50 g of shaded persimmon was refluxed for 3 hours with 70% (v / v) aqueous ethanol solution for 5 hours. After cooling, it was filtered with Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure at 50 DEG C or below and lyophilized to obtain 8.0 g of a Chinese cabbage extract.

Comparative Manufacturing Example  7: Manufacture of Viroxx extract

50 g of the shrubby pine needle was refluxed with 70% (v / v) aqueous ethanol solution for 5 hours three times, cooled, and filtered through Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower to obtain 9.4 g of a pine needle extract.

Comparative Manufacturing Example  8: Preparation of licorice extract

50 g of shredded licorice was refluxed for 3 hours with 70% (v / v) aqueous ethanol solution for 5 hours, followed by cooling and filtration with Whatman # 4 filter paper. Each of the filtered extracts was concentrated under reduced pressure and freeze-dried at 50 ° C or lower to obtain 9.4 g of licorice extract.

Experimental Example  One: Aquaporin  3 Biosynthesis Promotion Experiment

In order to measure the acceleration of the formation of aquaporin 3 in the samples prepared in Preparation Examples 1 to 3, the following experiment was conducted.

Human keratinocytes were inoculated on a 24-well plate (Falcon, USA) at a concentration of 1 × 10 ⁴ cells / well, and cultured for one day to stabilize the cells. To 8 were treated with 1% each and cultured in serum-free medium for 2 days. After incubation, the supernatant was taken and the absorbance at 460 nm was compared using aquaporin 3 assay kit (Aquaporin 3 ELISA KIT, Gill Blood Group, USA) to compare the degree of production of aquaporin 3 to determine the effect of each sample on the aquaporin biosynthesis Respectively.

Treatment concentration
(weight%) Degree of formation of aquaporin 3
(Absorbance at 460 nm) 0.5% One% 2% Comparative Preparation Example 1 0.115 0.315 0.511 Comparative Production Example 2 0.089 0.175 0.324 Comparative Production Example 3 0.11 0.234 0.421 Comparative Production Example 4 0.098 0.214 0.418 Comparative Preparation Example 5 0.089 0.155 0.398 Comparative Preparation Example 6 0.078 0.115 0.385 Comparative Preparation Example 7 0.121 0.201 0.412 Comparative Preparation Example 8 0.097 0.211 0.304 Production Example 1 0.485 0.924 1.134 Production Example 2 0.627 1.124 1.315 Production Example 3 0.607 1.116 1.357 No additives 0.121

As can be seen from the results of Table 1, it can be seen that the diagnosis of the cephalic arm of the present invention promotes biosynthesis of aquaporin 3 in keratinocytes. It is also confirmed that the fermented product of the diagnosis of the quartz arm also promotes biosynthesis of aquaporin 3. Compared with Comparative Production Examples 1 to 8, it can be confirmed that the acupuncture 3 biosynthesis promotion effect of the diagnosis of the quartz pulp is excellent.

Experimental Example  2: Pillar Green  Promoting effect of expression

Hacat cells were inoculated into 6 well plates. After 24 hours, the cells grown to 70-80% were replaced with serum-free DMEM and cultured for 5 hours. After culturing for 5 hours, Production Examples 1 to 3 or Comparative Production Examples 1 to 8 were treated. As a comparative example, a solvent (DMSO) was used instead of the production example. After further incubation for 16 hours, the cells were washed with PBS, total RNA was extracted with RNA prep kit (Qiagen, Germany), and the amount of RNA was standardized by UV absorbance and quantified using RT-PCR. The expression level of pilar green was compensated by GAPDH. The results are shown in Table 2.

Treatment concentration
(weight%) Expression rate (%) 0.5% One% 2% Comparative Preparation Example 1 107 115 142 Comparative Production Example 2 106 121 134 Comparative Production Example 3 107 121 134 Comparative Production Example 4 110 117 130 Comparative Preparation Example 5 108 118 132 Comparative Preparation Example 6 113 122 140 Comparative Preparation Example 7 108 117 134 Comparative Preparation Example 8 107 121 141 Production Example 1 141 161 224 Production Example 2 154 192 289 Production Example 3 151 187 291 DMSO 100

As shown in Table 2 above, it can be seen that the diagnosis of the quartz arm promotes gene expression of pilar green. It can be confirmed that the comparison of the concentrations of Comparative Production Examples 1 to 8 shows the excellent effect of promoting the expression of pillar green of the present invention.

Experimental Example  3: Inhibitory effect of melanin-forming cells on B16F1 melanin formation

Experimental Example 3 was conducted to determine the whitening effect of B16F1 melanin-forming cells by confirming the degree of inhibition of melanin formation to confirm the whitening effect of the diagnosis of the cephalic palate obtained in Production Examples 1 to 3 above. The B16F1 melanin-forming cell used in Experimental Example 3 is a cell strain derived from a mouse, and is a cell that secretes a melanin pigment called melanin.

During the artificial culture of these cells, samples were treated to compare the degree of reduction of melanin pigment. The B16F1 melanin-forming cells used in this experiment were purchased from the American Type Culture Collection (ATCC).

Melanin biosynthesis inhibitory effect of B16F1 melanin-forming cells was measured as follows.

B16F1 melanogenesis cells were plated at a concentration of 2 × 10 ⁵ per well in a 6-well plate, and the cells were incubated for 72 hours at a concentration that did not induce toxicity after treatment with the diagnosis of the cephalic arm according to Production Examples 1 to 3. After incubation for 72 hours, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Lotan (Cancer Res., 40: 3345-3350, 1980). Cell pellet was washed once with PBS, and 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin and the absorbance of melanin was measured at 405 nm with a microplate reader. The inhibition rate (%) of melanin formation was measured. Melanin formation inhibition rate (%) of B16F1 melanin-forming cells was calculated by the following formula (1), and the results are shown in Table 3 below.

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

Treatment concentration (% by weight) Melanin inhibition rate (%) 0.5 One 2 Comparative Preparation Example 1 5.4 21.2 42,5 Comparative Production Example 2 7.5 20.5 43.5 Comparative Production Example 3 6.4 19.5 42.2 Comparative Production Example 4 5.5 18.4 39.5 Comparative Preparation Example 5 7.9 20.1 37.6 Comparative Preparation Example 6 10.4 23.5 45.5 Comparative Preparation Example 7 9.1 22.4 40.9 Comparative Preparation Example 8 8.5 20.1 42.1 Production Example 1 35.45 50.43 70.15 Production Example 2 35.45 50.43 85.45 Production Example 3 37.45 53.43 84.65 Arbutin 200ppm 68.25

As can be seen from the results of Table 3, it was found that the purified ceiling-arm diagnoses prepared according to Production Examples 1, 2 and 3 of the present invention significantly inhibited melanin production in B16F1 melanin-forming cells. It can be confirmed that the melanin formation inhibiting effect of the diagnosis of the silkworm in the case of comparison with Comparative Production Examples 1 to 8 is excellent.

Experimental Example  4: Elastase  Activity inhibitory effect

The inhibitory effect of elastase activity on the diagnosis of the cephalic arm prepared in Examples 1 to 3 was measured.

To the 96-well plate was added 60 μl of a buffer (Tris buffer, pH 8.0), 20 μl of a substrate solution (Succinyl-Ala-Ala-Ala-p-nitroanilide (Sigma Aldrich, USA), and 20 μl of Porcine Pancreatic Elastase Sigma Aldrich, United States of America) and 100 μl of each of Production Examples 1 to 3 or Comparative Production Examples 1 to 8 were added thereto, followed by reaction at 25 ° C for 15 minutes. After the reaction, the absorbance was measured at 410 nm. In the control group, the reaction was carried out in the same manner using distilled water instead of the production example, and the absorbance was measured. The inhibition rate of elastase was determined by the following formula (2), and the results are shown in Table 4.

Treatment concentration (% by weight) Elastase activity inhibitory effect (%) 0.50% One% 2% Comparative Preparation Example 1 9.2 18.5 32.5 Comparative Production Example 2 8.9 20.1 31.2 Comparative Production Example 3 7.5 17.5 28.9 Comparative Production Example 4 8.5 18.5 30.1 Comparative Preparation Example 5 12 21.1 36.4 Comparative Preparation Example 6 10.3 20.5 33.5 Comparative Preparation Example 7 11.5 22.1 34.5 Comparative Preparation Example 8 10.5 21.1 37.5 Production Example 1 24.5 41.3 72.4 Production Example 2 34.9 52.4 82.6 Production Example 3 36.1 51 85.3 Distilled water 2.1

As can be seen from the results of Table 4, it was found that the purified cean-palm diagnoses prepared according to Production Examples 1 to 3 of the present invention significantly inhibited the activity of elastase. It can be confirmed that the elastase activity inhibiting effect of the diagnosis of the quartz pulp is excellent even when the concentrations are compared with those of Comparative Production Examples 1 to 8.

Experimental Example  5: DPPH Radical Scatters  analysis

In this Experimental Example 5, the antioxidative effect (free radical scavenging ratio) was measured using the DPPH method in order to measure the antioxidative effect of the cean-palm diagnosis obtained in Production Examples 1, 2 and 3. [ The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of reduction of the absorbance by reduction of DPPH by the test substance is compared with the absorbance of the blank test solution, and the free radical scavenging ratio (%) is measured at a wavelength of 560 nm. As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging ratio (%) were calculated according to Equation (3), and the results are shown in Table 5 below.

St: absorbance at 514 nm after free radical scavenging of the sample solution

Bt: Absorbance at 514 nm after free radical scavenging of blank test solution

So: the absorbance at 514 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 514 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample SC ₅₀ (wt%) Production Example 1 0.025 Production Example 2 0.01 Production Example 3 0.02 Comparative Preparation Example 1 0.15 Comparative Production Example 2 0.2 Comparative Production Example 3 0.35 Comparative Production Example 4 0.15 Comparative Preparation Example 5 0.25 Comparative Preparation Example 6 0.4 Comparative Preparation Example 7 0.55 Comparative Preparation Example 8 0.25

The SC ₅₀ , which is the concentration of the sample required to remove 50% of the DPPH free radical, was confirmed. It can be confirmed that the antioxidative effect is excellent even in comparison with Comparative Production Examples 1 to 8.

Experimental Example  6. MMP -1 production inhibitory effect

In Experimental Example 6, the effect of inhibiting the production of collagenase, MMP-1, in the cases of the cephalometric diagnosis obtained in Production Examples 1, 2 and 3 was measured.

Human normal skin cells were inoculated into a 48-well microplate (Nunc, Denmark) at a concentration of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, USA) and 37 ° C For 24 hours and then cultured in serum-free DMEM medium and serum-free DMEM supplemented with Comparative Production Examples 1 to 8 of Preparation Examples 1 to 3 and control group for 48 hours for 48 hours Respectively.

After the incubation, the supernatant of each well was pooled and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 6.

Treatment concentration (% by weight) Inhibition rate of MMP-1 production (%) 0.50% One% 2% Comparative Preparation Example 1 8.1 17.1 31.5 Comparative Production Example 2 7.8 18.5 30.5 Comparative Production Example 3 7.6 15.4 28.7 Comparative Production Example 4 7.1 13.4 28.4 Comparative Preparation Example 5 8.4 19.3 30.5 Comparative Preparation Example 6 10.3 20.4 37.4 Comparative Preparation Example 7 11.4 22.4 38.5 Comparative Preparation Example 8 10.8 21.6 29.1 Production Example 1 43.74 67.57 77.12 Production Example 2 52.81 77.23 87.19 Production Example 3 54.14 78.55 89.51 TGF-β 200 nM 76.8

As shown in the results of Table 6 above, it was confirmed that the diagnosis of the cephalic arm of the present invention inhibits MMP-1 production in a concentration-dependent manner. Compared with Comparative Examples 1 to 8, it can be confirmed that the effect of inhibiting MMP-1 production of the cephalic arm diagnosis is excellent even when the concentrations are compared.

Experimental Example  7. Enhancement of collagen synthesis

Human normal epithelial cells, fibroblasts, were inoculated into 48-well microplates at a density of 1 x 10 ⁶ cells per well and cultured in DMEM medium for 24 hours. The cells were further cultured in serum-free DMEM medium prepared by Preparation Examples 1, 2 and 3, and serum-free DMEM medium containing no cephalic arm diagnosis for 48 hours as a control group. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following equation (5), and the results are shown in Table 7.

Treatment concentration (% by weight) Collagen biosynthesis rate (%) 0.50% One% 2% Comparative Preparation Example 1 15.7 25.4 45.7 Comparative Production Example 2 12.1 20.6 38.5 Comparative Production Example 3 8.9 15.4 30.5 Comparative Production Example 4 14.5 21.2 46.5 Comparative Preparation Example 5 11.5 19.8 35.4 Comparative Preparation Example 6 16.7 24.5 48.5 Comparative Preparation Example 7 12.6 27.1 37.5 Comparative Preparation Example 8 15.4 28.3 43.5 Production Example 1 81.5 107.8 141.4 Production Example 2 109.5 139.1 171.5 Production Example 3 105.1 141.8 177.1 TGF-beta (200 nM) 168.7

According to Table 7, it was confirmed that the collagen biosynthesis rate of the purified cephalic collagen prepared by Production Examples 1 to 3 of the present invention increased in a concentration-dependent manner. In particular, it can be confirmed that the effects of the extracts prepared in Production Examples 2 and 3 are better than those of the production examples. In addition, it can be confirmed that the inhibitory effect on the MMP-1 production of the cephalic arm diagnosis is excellent even when the concentrations are compared with those of Comparative Production Examples 1 to 8.

Formulation Example  One : The diagnosis of the  Containing Cosmetics  Preparation of composition

Cosmetic preparations containing a diagnosis of a quartz arm were prepared in the same manner as the cosmetic preparations (Formulation Examples 1, 2 and 3) each containing the diagnosis of the cephalic arm of Production Examples 1 to 3. For comparison of efficacy, glycerin and 1,3- (Comparative Formulation Example 1), a cosmetic composition containing adenosine as a wrinkle improving material (Comparative Formulation Example 2), a cosmetic composition not containing both an extract and a moisturizer (Comparative Formulation Example 3), and a cosmetic composition (Comparative Formulation Example 4) containing niacinamide as a whitening material instead of a quartz-based diagnosis was prepared as shown in Table 8 below.

division ingredient Formulation Example
One Formulation Example
2 Formulation Example
3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 compare
Formulation Example 4 compare
Formulation Example 5 compare
Formulation Example 6 A Diagnosis of a cephalic arm
(Production Example 1) 2.0 - - - - - - - - Diagnosis of a cephalic arm
(Production Example 2) - 2.0 - - - - - - - Diagnosis of a cephalic arm
(Production Example 3) - - 2.0 - -  -  -  -  - Comparative Production Example 2 - - - - - - - 2.0 - Comparative Production Example 3 - - - - - - - - 2.0 glycerin - - - 5.0 - - - - - 1,3-butylene glycol - - - 5.0 - - - - - Adenosine - - - - 3.0 - - - - Niacinamide - 2.0 - 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 B Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 C Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example  8: Moisturizing effect

The clinical moisturizing effects of the cosmetic compositions of Formulation Examples 1 to 3 were measured as follows. A, B and C groups were divided into five groups (A, B, C, D, E and F) by randomly dividing 100 healthy men and women, (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as moisturizing agents instead of the diagnosis of the heavenly palms in the D group, cosmetic compositions containing no cosmetic agent and humectant in the E group The composition (Comparative Formulation Example 3) was applied to the face of the cosmetic composition containing Comparative Preparation Example 3 (Comparative Formulation Example 6) twice a day for 8 weeks in the F group. The moisturizing effect was evaluated by Corneometer CM 820 (Corage + Khazaka, Germany) for every 2 weeks and after the start of the test. The experimental results are shown in Table 9 below.

division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 21.6 33.9 51.3 Formulation Example 2 23.7 38.8 58.7 Formulation Example 3 23.1 40.5 59.5 Comparative Formulation Example 1 23.5 37.4 46.7 Comparative Formulation Example 3 22.1 24.7 23.8 Comparative Formulation Example 6 22.2 27.7 37.8

As shown in Table 9 above, it was confirmed that the cosmetic compositions (Formulation Examples 1 to 3) of the present invention having the diagnosis of the present invention had excellent moisturizing effect as compared with the cosmetic compositions containing other moisturizing agents (Comparative Formulation Example 1).

Experimental Example  9: Quartz Whitening effect

Clinical experiments were carried out on the skin whitening effect of cosmetics containing the diagnosis of the present invention of the present invention. Clinical trials were conducted through actual use tests of each cosmetic product.

100 women (30 to 50 years old) were divided into experimental group (20 patients each), treatment group (glycerin and 1,3-butylene glycol) as a moisturizing agent (Comparative Formulation Example 1) (Formulation Example 2), which contains the experimental group of Formulation (Formulation Example 1) containing Preparation Example 1, Preparation Example 2, Preparation Example 3 (Comparative Example 4) (Comparative Formulation Example 6), and the brightness (L *) was measured with a Chromameter (Minolta CR300) using both sides of the face. And the whitening effect after 4 weeks was confirmed. The results of the whitening effect based on this evaluation are shown in Table 10 below.

division The measured value (L *) Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 58.6 61.5 64.3 Formulation Example 2 58.7 36.8 66.7 Formulation Example 3 58.6 37.5 65.8 Comparative Formulation Example 1 58.4 58.7 59.0 Comparative Formulation Example 4 58.3 61.3 63.8 Comparative Formulation Example 6 57.9 59.3 61.8

As shown in Table 10, it was confirmed that the whitening effect of the cosmetic compositions (Formulation Examples 1 to 3) contained in the present invention of the present invention was excellent in comparison with the cosmetic containing the whitening component (Comparative Formulation Example 4).

Experimental Example  10: Quartz  Wrinkle improvement effect

Clinical experiments were carried out on the effect of improving the skin wrinkles of cosmetic compositions containing the diagnosis of the present invention of the present invention. Clinical trials were conducted through actual use tests of each cosmetic product.

That is, the clinical tests were conducted in the same manner as in Formulation Examples 1, 2, and 3, which contain the diagnosis of the cephalic arm of Preparations 1 to 3 in Table 8, and the comparative formulations containing glycerin and 1,3- 1, a cosmetic composition of Comparative Formulation Example 2 containing 3% by weight adenosine instead of a quartz arm diagnosis, and a cosmetic composition of Comparative Formulation Example 5 containing Comparative Preparation Example 2 instead of a cephalometric diagnosis, The effect of improving skin wrinkles was evaluated and compared.

A total of 120 women (30 to 50 years old) were divided into 20 experimental groups, each of which was treated with a formulation containing glycerin and 1,3-butylene glycol as a moisturizing agent (Comparative Formulation Example 1) (Comparative Formulation Example 2) treatment group in which the cephalometric diagnosis was replaced by Comparative Preparation Example 2 (Comparative Formulation Example 5), the experimental group of Formulation (Formulation Example 1) containing Preparation Example 1 (Formulation Example 2) containing Preparation Example 2, and the experimental group of Formulation (Formulation Example 3) containing Preparation Example 3, and the wrinkle improvement effect after 6 weeks using both sides of the face was evaluated The degree of wrinkle improvement was visually evaluated. The wrinkle-improving effect results based on this evaluation are shown in Table 11 below.

The effect of improving wrinkles after the completion of the experiment was evaluated by visual examination and instrument measurement (cutometer SEM 575, C + K Electronic Co., Germany) of a skilled physician before and after 3 months of use. The experimental results are shown in Table 11 below.

Wrinkle-improving effect of cosmetic composition including a diagnosis of a quartz arm (visual evaluation and device evaluation) Wrinkle improvement effect Effective rate (%) Great slightly none Formulation Example 1 9 7 4 80.0 Formulation Example 2 11 7 2 90.0 Formulation Example 3 13 6 One 95.0 Comparative Formulation Example 1
(Moisturizer) 2 4 14 30.0 Comparative Formulation Example 2
(Adenosine) (11) (6) (3) (85.0) Comparative Formulation Example 5
(Comparative Production Example 2) (5) (7) (8) (60.0)

As can be seen from the results of Table 11, the cosmetic compositions of Formulation Examples 1 to 3 containing the diagnosis of the present invention showed a wrinkle improvement effect about 2.6 times higher than Comparative Formulation Example 1 containing moisturizing agent.

In addition, similar results were obtained with the cosmetic preparation of Comparative Formulation Example 2 containing adenosine, which is known to have a wrinkle-reducing effect instead of the diagnosis of a quartz arm, and the diagnosis of the quartz arm shows excellent wrinkle-reducing effect.

Experimental Example  11: Measurement of skin elasticity improvement effect

Experimental Example 11 is a comparative example 1 in which Formulation Examples 1 to 3 including the diagnosis of the cephalic palate obtained in Production Examples 1 to 3, Comparative Formulation Example 1 in which a moisturizer is put in place of the diagnosis of the cephalic arm and Comparative Formulation Example 5 To evaluate the skin elasticity improvement effect of the cosmetic composition according to the present invention. 10, 5, 5, 10, 10, 10, 10, 10, 10, 10, 10, 10, Was applied to the face in the morning and evening for 12 weeks, and then skin elasticity was measured after 12 weeks. The elasticity of the facial region was measured using a cutometer SEM575 (MPA 580, Courage + Khazaka GmbH, Germany), and the R2 (biological elasticity) indicating skin elasticity was measured before and after application . The results are shown in Table 12 below, and the results are an average value of improvement for each group.

Experimental product Skin elasticity improvement (%) Formulation Example 1 25.8 Formulation Example 2 30.5 Formulation Example 3 31.1 Comparative Formulation Example 1 6.4 Comparative Formulation Example 5 15.9

As shown in Table 12, it was found that Forms 1 to 3 containing a diagnosis of a quartz arm greatly improved skin elasticity as compared with the comparative formulation.

Experimental Example  12: Dermal density  Measure improvement

Clinical experiments were carried out on the effect of improving the dermal density of cosmetics containing the diagnosis of the present invention. Clinical trials were conducted through actual use tests of each cosmetic product.

That is, the clinical tests were conducted in the same manner as in Formulation Examples 1, 2, and 3, which contain the diagnosis of the cephalic arm of Preparations 1 to 3 in Table 8, and the comparative formulations containing glycerin and 1,3- Comparative formulation No. 2 containing adenosine 3% by weight instead of the diagnosis of cephalic palm, Comparative formulation 5 containing Comparative preparation example 2 instead of the cephalic arm diagnosis, Were identified and compared.

A total of 120 women (30 to 50 years old) were divided into 20 experimental groups, each of which was treated with a formulation containing glycerin and 1,3-butylene glycol as a moisturizing agent (Comparative Formulation Example 1) (Comparative Formulation Example 2) treatment group in which the cephalometric diagnosis was replaced by Comparative Preparation Example 2 (Comparative Formulation Example 5), the experimental group of Formulation (Formulation Example 1) containing Preparation Example 1 , The experimental group of formulation 2 (preparation example 2) containing preparation example 2 and the experimental group of formulation (formulation example 3) containing preparation example 3, and the dough density improvement after 4 weeks using both sides of the face The effects were confirmed by instrument evaluation using SkinScanner DUBcutis (tpm (terbana pro medicum), Luneburg, Germany). The dermal density increase rate was calculated according to Equation (6) below, and the dermal density improvement effect was shown in Table 13 below.

Experimental product Density increase rate of dermis (%) Formulation Example 1 38.8 Formulation Example 2 45.5 Formulation Example 3 47.1 Comparative Formulation Example 1 3.4 Comparative Formulation Example 2 39.1 Comparative Formulation Example 5 18.1

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (9)

Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
The cosmetic composition having an antioxidative effect, wherein the herbal extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
Wherein the herbal medicine extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
The cosmetic composition according to any one of claims 1 to 3, wherein the herbal extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
Wherein the herbal medicine extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
Wherein the herbal extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. Containing as an active ingredient an extract of Chinese medicinal herb including Gugija, Ginseng, Hwanggi, Licorice, Omija, Hwangjeong,
Wherein the herbal medicine extract is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition, and has a skin dermis compacting improving effect. The herbal medicine extract according to any one of claims 1 to 6, which comprises (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, A solvent extraction method using an extraction solvent selected from the group consisting of butyl acetate, diethyl ether, dichloromethane, hexane, and mixtures thereof, or (b) an extraction method using fermentation or natural fermentation metabolism from microorganisms Cosmetic composition. delete delete