KR101998685B1 - A cosmetic composition comprising arenaria serpyllifolia extract - Google Patents

Abstract

The present invention provides a cosmetic composition comprising a flea spot extract as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising a flea-zyme extract as an active ingredient,

The present invention relates to a cosmetic composition comprising a flea spot extract as an active ingredient.

Skin is an important organ that protects the body from physical, chemical, or biological stimuli. In addition, the skin plays an important role in maintaining the homeostasis of the body such as controlling moisture and body temperature inside the skin.

However, as the skin gets older, it undergoes changes due to a number of internal and external factors. As the secretion of various hormones that regulate the metabolism inside the skin decreases and the function of the immune cells and the activity of the skin cells decrease, various troubles such as inflammation and atopy occur in the skin. In addition, free radicals and active noxious oxygen are increased due to deterioration of skin function, melanin is deposited in cells, and skin is damaged by spots, freckles and wrinkles.

In order to improve such skin, various cosmetic compositions which are external preparations for skin have been developed. In particular, functional cosmetic compositions include antioxidants that remove free radicals and active noxious oxygen which are the causes of oxidative stress. In general, the antioxidant of cosmetics has the ability to significantly reduce or prevent the destruction and depletion of skin functions and structures damaged by oxidation, and to restore damaged skin due to ultraviolet exposure.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials. Especially, medicinal plants contain various functionalities including anticancer effect and are attracting attention as a raw material for cosmetics

However, the cosmetic product containing the extract derived from natural origin obtained by a conventional method has insufficient functionalities such as antioxidation, anti-inflammation and whitening, and has a problem that the skin improving activity is not maintained constantly. Accordingly, there is a continuing need to develop cosmetic products derived from natural products, which have excellent functionalities and excellent biostability and skin improving effect.

The flea is a medicinal plant that grows frequently in roads, gutters, and grasses. It is used for fever, sore throat, etc. and is eaten with young pure spring herbs. It is known that the flea spot has a beneficial effect on cyanosis, nematode, detoxification, periodontitis, acute conjunctivitis and sore throat, but it is not widely used for skin improvement.

On the other hand, it is called fermentation that a substance useful for living is produced in the process of decomposing organic matter using an enzyme possessed by a microorganism. Natural products fermented through useful microorganisms are known to multiply biologically active ingredients more than before fermentation. The physiologically active ingredients contained in natural products are various amino acids, organic acids and natural antioxidants that are beneficial to the skin. In addition, if the particles of the natural product become smaller through the fermentation process, the absorption rate is good and the skin troubles are alleviated. Since excellent fermentation strains can metabolize natural products and produce new physiologically active ingredients that have not been present, various functional effects can be exhibited by fermenting natural products.

Therefore, in view of the necessity of development of natural products derived from natural products having superior biocompatibility and skin improving effect, the present inventors have sought to develop a functional cosmetic composition using fleas.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cosmetic composition having excellent functionality including a flea spot extract.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a flea-bearing spot extract as an active ingredient.

In one embodiment, the Flea sp. Extract may be a fermentation extract prepared by inoculating a fermentation strain.

In one embodiment, the fermentation strain is selected from the group consisting of Hericium erinaceum , Pleurotusus ferulae), Blossom Mushrooms (Sparassis crispa , Ganoderma lucidum , Flammulina velutipes , Phellinus linteus , and Grifola frondosa .

In one embodiment, the cosmetic composition may further comprise at least one member selected from the group consisting of cloud mushroom extract and mushroom extract.

In one embodiment, the cosmetic composition is selected from the group consisting of Puerariae Radix extract, Licorice root extract, Ganoderma lucidum extract, Radix extract, Radix extract, Radix extract, Radix extract, Radix extract, Radix extract, Radix extract, The extract may be at least one selected from the group consisting of mulberry leaf extract, mulberry leaf extract, mashin extract, golden extract, Angelica keiskei extract, Rhodiola extract,

In one embodiment, the cosmetic composition may be for antioxidant and whitening.

In one embodiment, the extract of the cosmetic composition comprises water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, -Butylene glycol. ≪ / RTI >

In one embodiment, the cosmetic composition is selected from the group consisting of softening longevity, nutritional lotion, water cream, nutritional cream, massage cream, nutritional lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, Shampoo, rinse, pack, spray, and powder.

The cosmetic composition according to one aspect of the present invention is excellent in antioxidative and whitening effects by containing a flea spot extract.

In particular, since the content of the polyphenol compound as an antioxidant increases as the flea fermentation extract is fermented, the antioxidant effect of the cosmetic composition is excellent.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIG. 1 is a graph showing the whitening effect of the composition prepared according to an embodiment of the present invention.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a flea-bearing spot extract as an active ingredient.

The cosmetic composition of the present invention contains an extract of natural origin as an active ingredient, so that it is excellent in skin improving effect and biosafety, and skin irritation can be minimized.

The above-mentioned " comprising as an active ingredient " is intended to mean an effective amount of a composition capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin whitening, skin irritation alleviation, It can mean to do.

The " Arenaria serpyllifolia ") is a biennial plant belonging to the family Seokjuk. The stem of the flea is 10-25 cm in height and thin, and branches are split much at the base, the leaves are opposite, ovate or long round. The flea spot is effective in treating acute conjunctivitis and fever accompanied by fever, clearing the eyes, and fever.

In one embodiment, the Flea sp. Extract may be a fermentation extract prepared by inoculating a fermentation strain.

The term " fermentation " as used herein means a process of decomposing an organic substance using an enzyme of the fermentation strain itself. It is known that raw materials after fermentation through fermentation strains are at least two times to several tens of times more effective than before fermentation. Fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for skin, promoting skin metabolism and smooth skin texture. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed, so that the absorption rate is good and the skin troubles and allergy side effects are alleviated.

The "fermentation extract" used in the present invention is prepared by sterilizing the fleas, inoculating the fermentation broth, fermenting the mixture at a predetermined temperature for a predetermined period of time, and then fermenting the fleas, and extracting the fleas with a specific solvent, preferably ethanol .

The fermentation strains were obtained from Hericium < RTI ID = 0.0 > erinaceum , Pleurotusus ferulae , Sparassis crispa , Ganoderma lucidum , Flammulina velutipes ), mushroom ( Phellinus linteus and Grifola frondosa . However, the mycelium may be one or more selected from the group consisting of, but not limited to, Ganoderma lucidum .

The cosmetic composition may further comprise at least one member selected from the group consisting of cloud mushroom extract and mushroom extract.

The " cloud mushroom ( Trametes versicolor "is a mushroom that belongs to the mushroom family of the mushroom throat. Often it is also called fungi or fungus mushroom. It is distributed all over the world and grows all year round on trees and rocks of coniferous and broad-leaved trees. The cloud mushroom is a medicinal mushroom in which an anticancer component is first discovered. The polysaccharide K (PSK) found in the extract of the cloud mushroom is used in research related to cancer treatment, delaying the transfer of cancer cells, enhancing immunity of patients who received chemotherapy, To some extent.

The above " shiitake mushroom ( Lentinula edodes ) "is a mushroom of wrinkled mushroom neck. The shiitake mushrooms occur in dry trees of broad-leaved trees such as oak, chestnut and acorn trees in spring and autumn. The shiitake contains eridademine, which has the effect of eliminating cholesterol and lowering blood pressure, thereby alleviating cardiovascular diseases such as hypertension and arteriosclerosis. In addition, the mushroom contains a large amount of vitamins B1, B2 and B12 useful for the skin.

The cosmetic composition according to the present invention can be used as a cosmetic composition for a cosmetic composition which comprises at least one composition selected from the group consisting of Puerariae Radix, Licorice Root Extract, Kwangwha Root Extract, Root Extract, Root Extract, Leaf Extract, Root Extract, Root Extract, Root Extract, The extract may further comprise at least one selected from the group consisting of extracts of Lycoris spp., Extracts of Lycoris spp., Gold extracts, Angelica keiskei extract, Rhodiola extract, Lycoris spp.

The above-mentioned " Puerariae radix " is a root of Puerarialobata Ohwi according to the Korean Pharmacopoeia, and is widely used as a food, beverage, and health food in the private sector. It is used for the purpose of breaking down cold body, digestive diseases, and stomach cramps. It helps the spleen and stomach to rise, so it can help to relieve thirst and make a branch. .

The "licorice ( Glycyrrhiza uralensis Fischer "is a plant which is also called kuno (國 老), Micho (草), mandarin (蜜 甘), wax (蜜 草), Youngtong (靈通) Is reddish brown or dark brown with vertical wrinkles, occasionally shrubs, buds, scaly leaves, unusual smell and taste. In one room, harmful effects of all drugs are harmonized, It is known to rule over all blood vessels, strengthen muscles and bones.

The " Farfarae flos ) "is a perennial flower belonging to Asteraceae. There is a characteristic of the lungs in the room, and it is characterized by the darkness and the drowning. Especially when the lungs are coughing and the blood is mixed in the pharynx and pulmonary tuberculosis and lung abscess are effective It is known. In addition, it is widely applied to seawater, asthma, and upper respiratory tract infections caused by external sensation.

The " Sophora flavescens "is a perennial herb with rosemary roots and twigs. It is also known as the caterpillars of thieves, foxes, and snakes. The roots that are peeled off from the ginseng are well known as raw materials showing skin fungus inhibition, skin moisturizing, whitening, blood circulation improvement as well as preservative effect. Gosam is effective for jaundice, dysentery, dysentery, and genital itching caused by Hachohe fever (fever fever). In itching, it cleanses the affected area with water. It is used for skin itching, It is known that the symptoms show signs of illness.

The " Magnolia officinalis " is an evergreen broad-leaved tree belonging to the dicotyledonous plant, and is an evergreen broad-leaved tree belonging to the family Ranunculaceae. It lives in Korea, Japan, Taiwan, and southern China. As a medicinal herb, stem and egg shells have been used in the treatment of asthma and gastrointestinal disorders and contain ingredients such as magnolol, isomagnolol, honokiol, and machiol. Among them, honokiol belongs to polyphenol and is used as a useful substance showing antidepressant, thrombolytic inhibition, nerve stabilization and antibacterial activity.

The "white" is a tuber of the grown ( Bletilla striata Reichb. Fil. ) . It contains 14.6% moisture, 30.48% starch and 1.5% glucose in the fresh raw meat and contains essential oil and mucus. The roots contain bletilla mannan. Bacillus has a good local hemostatic effect and coagulates blood cells to form an artificial thrombus. It has antibacterial action and is known to be used for the treatment of pulmonary tuberculosis and complications of tuberculosis and pulmonary tuberculosis.

The " Rhus Semialata "is a wormhole formed by the parasitic bugs on leaves of Rhus verniciflora (Rhus verniciflua) with rhododendron larvae, and the appearance is uneven, irregularly split into 2 ~ 4 pockets or broken. Outer side is grayish grayish brown, covered with light brown short hair, hard and brittle. The genus is empty, but grayish white color or dead worms and secretions may remain. It is used to treat chronic seawater, old cough, old diarrhea, diarrhea, cold sweat, oil wells, which are caused by the strong convergence of the lungs, and to treat fecal bleeding, nosebleeds, uterine bleeding, trauma bleeding, early dermatitis and itching Has come. The pharmacological effect is effective for the convergence effect, the antibacterial action, the hepatic function-protecting action, and the antioxidative action.

The " Eleutherococcus " Senticosus ( Rupr . & Maxim.) Maxim .) "grows in Mt. Odaesan, Jirisan, etc., Manchuria, Siberia, Hokkaido, etc. It is said that it promotes the enhancement of the overall function of the living organism, have.

The above-mentioned " Persiccae Semen " is a mature seed of a peach tree belonging to the rose family. It is a fat seed oil (40-50%), amygdalin, triolein, water-soluble proteins PR-A and PR- al., Shoyakugaku Zasshi 40, 2, 129, 1986) and is known to be used for the treatment of anticoagulant blood, gonadal, intestinal lubrication and stagnant blood.

The " Liriope Platyphylla "is a perennial plant that grows rhizomes and seeds. It is mainly distributed in low hills below 500 m in Korea, and the leaves are green throughout the year (Huh, MK, HW Huh, JS Choi, and BK Lee 2007. Genetic diversity and population structure of Liriope platyphylla (Liliaceae) in Korea. J. Life Sci. 17, 328-333.). McMundong has been widely used in Korea and China for the treatment of diseases such as coughs and phlegm, and recently it has been known to be effective for treating diabetes, improving memory, inhibiting microorganisms, and inhibiting inflammation.

The above-mentioned " Panax ginseng " is a plant growing in a deep mountain area, reaching a height of 60 cm, short rhizome, standing straight or obliquely, and bellflower-like roots developing from the bottom. It is widely cultivated in Korea and China. Ginseng is known to refresh the body and to help people with weakness, boredom, fatigue, anorexia, vomiting and diarrhea.

The " Cnidium " officinale Makino ) "is a perennial herb that belongs to the persimmon plant. It is grown in China, Korea, and Japan as the origin of China. The physiological functions of the celesta are antioxidant (Lee et al., 2002) and platelet aggregation inhibitory activity (Zhang L et al., 2009).

The " Cyperi Rhizoma "is the root of the seedling and perennial herb. It contains essential oil, alkaloid, flavonoid, sugar, pectin and so on. Fluid X of rosemary rootstock is known to improve analgesia, relieve tension of the uterus, improve heart function, improve urination and improve blood circulation. The herbal medicine in one room is widely used for menstrual irregularities due to menstrual irregularities, menstrual irregularities, and postpartum women's gynecological diseases. It is also used for cold, headache, stomach pain, etc. With regard to cosmetics, herbal supplements have a strong antimicrobial effect against skin fungi, smooth blood circulation, make skin color rich, and prevent skin aging caused by antioxidative and anti-inflammatory actions.

The "Mori Cortex" is mulberry (Morus Alba ) or dried root bark of the same genus, and has been widely used in oriental herbs since ancient times as anti-inflammatory, anti-inflammatory and diuretic. It is known that the manganese bran is excellent in analgesic, diuretic, anti-seizure, anti-edema, sedative, anti-convulsive and hypotensive effects and has been used for dandruff, itching and hair loss related diseases.

The " Perilla " ocymoides "is a perennial herb that belongs to the family Lamiaceae. It is used in late summer to cover and shade it in the shade. Its flavor is spicy and its quality is warm, and it acts on menopause, nausea , and cicada . The lobular lobes sweat out to clear the wind, smooth the stomach, stabilize the fetus and decipher the fish poison. In the pharmacological experiments, weak fever, arthritis, insomnia, and side effects were found. In addition, the lobular lobes are used for vaginal lavage, nausea and vomiting, dryness, vomiting, vomiting of the pregnant woman, sudden onset anxiety due to gas and fish poisoning.

The above-mentioned " Portulaca oleracea " is an annual plant belonging to the periwinkle of the leggings of the leggings, the leggings of the leggings, and the leggings of the leggings. The mashin is known to have an effect on detoxification, diuretic and dysentery in pharmacological activity, and it is known to be effective on detoxification, diuretic and dysentery, It is also called horsemeat.

The " golden ( Scutellaria baicalensis ) is a perennial herbaceous plant belonging to the family Lamiaceae. It grows in the grasslands of the mountainous region and is 20 ~ 60cm in height. The roots of gold are used as an antipyretic, diuretic, branching, insect and anti-inflammatory agent in oriental medicine. In addition, the gold contains bacalin, one of the flavonoids, and is known to have antioxidant, anti-cancer and anti-inflammatory effects.

The " Angelicae Gigantis Radix ) "is a perennial herbaceous perennial herbaceous plant belonging to the genus Ganoderma. It is known to grow in mountainous regions and highlands in the northern part of Korea and has long been used for medicinal and edible purposes. The Angelica gigas include coumarins, essential oils, and polyacetylenes. Major active ingredients include decursin, decursinol angelate, and the like. It is known that the herbal medicine has an effect on the antimicrobial effect, the fatigue recovery and the blood, the anemia and the female lineage system by the pharmacological effect.

The " Coptis & chinensis ) "is a perennial herb that belongs to the Mineranidae. It is known that the phloem is composed of alkaloid compounds such as berberine, coptisine, and palmatine, and components such as obacunone and obaculactone. Antimicrobial activity, hypotensive effect, and relaxation of tracheal smooth muscle such as uterus are known.

The " Flos Sophora japonica Linne ) "is a blossom and flower peony of beans and plants belonging to a pictorial tree belonging to the two families. It is a commodity (commodity) in the Divine Materia Medica along with an umbrella. It is a pharmacologically-based taste of the herbaceous pharmacologically, and the nature is cool, and the liver and the large intestine return to the fever to cool down the blood, to stop the bleeding, to tremble by the bleeding of the eyes Red), hypotensive effects and stroke. In addition, it has been known that the above-described seedling has an activity of promoting growth of dermal papilla cells and human keratinocytes, thereby promoting hair growth and healthy scalp growth.

The "yellowish (Phellodendron amurensis ) "is a term used to refer to the skin of an Asahi tree as a medicinal herb, also known as the" It is distributed in all regions except Jeju and Jeonnam regions in Korea, and is distributed in Japan, Manchuria, China, Amur and Sochi, and inhabits in forests or mountain valleys. It is known that the skin is peeled off from the stem of the yellowish white to remove the rough skin, or it is sliced and dried in the sun, and then takes the medicine and lowers the blood sugar.

The above " Polygonum Multiflorum ) "is a perennial herbaceous, vine medicinal plant belonging to the family Mallardiidae. It is used as a tonic, gangjeongje, tongkuk economy, hemicellulant, and chinhaeje, and it is known to be used for the treatment of cold, hematemesis, nervous breakdown and arthritis.

The "Houttuynia cordata (Houttuynia cordata thunb . ) Is a prey of the seedling, a plant of Saururus chinensis, which lives in southeastern Asia and especially in Japan and Korea. It is pharmacologically known to have strong cardiac, diuretic, antibacterial, detoxifying and anti-cancer efficacy, and is used as a cosmetic and health functional food for detoxification and beauty in the private sector. It is known that there are many decanoyl acetaldehyde compounds and antioxidants, antioxidants, antifungal compounds and flavonoid compounds which are effective for diuretic, vasoconstriction and defecation.

The " extract " may include both the extraction method and the kind of the ingredient, provided that the ingredient contained in the raw material is isolated from the natural substance as the active ingredient contained in the extracting raw material by contacting the solvent and the extracting raw material under specific conditions .

For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil. The extract may be dried and pulverized, or may be obtained by various extraction methods known in the art such as a hot water extraction method and an ethanol extraction method.

Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol (V / v) ethanol. The concentration of the solvent may be in the range of about < RTI ID = 0.0 > 60% < / RTI >

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect Can

Since the water and ethanol have different polarities and the effective components to be extracted depend on the polarity, the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of ethanol is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently, and if it is more than 80%, an adequate yield may not be realized.

The extract is washed with water, dried and pulverized, and extracted and extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, Followed by filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The extract can be extracted for 12 to 18 hours at a temperature of 50 ° C to 70 ° C using the ethanol as a solvent.

On the other hand, if the extraction temperature is lower than 50 ° C, the extraction efficiency may be lowered and the active ingredient may not be eluted properly. If the extraction temperature is higher than 60 ° C, the active ingredient contained in the natural raw material may be deformed or lost at high temperature, Therefore, it is preferable to keep the extraction temperature within an appropriate range. If the extraction time is less than 12 hours, the active ingredient contained in the natural raw material may not be completely extracted. If the extraction time exceeds 18 hours, the content of the remaining active ingredient contained in the natural raw material is inadequate, It is preferable to control the extraction time appropriately.

The cosmetic composition may be for antioxidant and whitening.

The above-mentioned " antioxidant " means an oxidation-inhibiting action. When the human body is balanced with the oxidation-promoting substance and the oxidation-inhibiting substance, due to various factors, such a balance state is lost, , And oxidative stress in the body may be induced, leading to cell damage and pathological disease.

Reactive oxygen species (ROS), which is a direct cause of oxidative stress, are chemically unstable and highly reactive and can easily react with various biomaterials such as DNA, proteins, lipids and carbohydrates, Causing irreversible damage to cells and tissues, causing mutations, cytotoxicity and cancer, and can also be a direct cause of aging. By removing or reducing the active oxygen species, an antioxidant effect can be obtained to prevent aging and maintain health.

The "whitening" suppresses the formation of various biomolecules such as melanin, redox hemoglobin, carotene and melanoid which affect skin color, thereby alleviating skin pigmentation phenomena such as dullness, freckles and stains, Thereby improving skin brightness and uniformity.

The cosmetic composition of the present invention can be used as a cosmetic composition for cosmetics such as softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, A spray, a powder, and a powder.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. In addition, the external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Production Example 1: Preparation of natural extract

After washing the flea spot, cloud mushroom, shiitake mushroom, sorghum, yellow whites, daphnia magna, and whitewash, it was thoroughly dried at room temperature and pulverized to obtain 100 g of pulverized material. 100 g of each pulverized product was immersed in a 20-fold volume of 80% ethanol as a solvent for 7 days at room temperature.

The extract was filtered through a 250-mesh and 3-μm filter, concentrated under reduced pressure at 40 ° C, and lyophilized to obtain a solid fraction of the extract.

Production Example 2: Preparation of Flea Nodule 1st Fermentation Extract

After the fleas were rinsed with water, they were completely dried at room temperature and pulverized to obtain 100 g of each of the pulverized products. The above-mentioned alabaster and the crushed lysates were autoclaved at 121 DEG C for 120 minutes, respectively, and then the fermentation strain Ganoderma lucidum ) were inoculated at 10% (w / v).

After the flea inoculated with the fermentation strain was cultured at 30 ° C for 12 days, the fermented fleas were immersed in a 10-fold volume of ethanol for 12 hours at a temperature of 50 ° C.

The extract was filtered by a 250 mesh filter and a 0.5 탆 filter and repeatedly extracted three times in the same manner for the remaining raw materials. The filtrate was concentrated under reduced pressure at 30 ° C and lyophilized to obtain a solid fraction of each of the flea-leaf fermentation extracts, .

Production Example 3: Preparation of Flea Nodule 2nd Fermentation Extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Hericium erinaceum .

Production Example 4: Preparation of the third fermentation extract of Flea sp.

A fermented extract was prepared in the same manner as in Preparation Example 2, except that the fermentation strain was changed to Pleurotusus ferulae .

Production Example 5: Preparation of Flea Nodule Fourth Fermentation Extract

A fermented extract was prepared in the same manner as in Preparation Example 2 except that the fermentation strain was changed to Sparassis crispa .

Production Example 6: Preparation of Flea Nodule Fifth Extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Flammulina velutipes .

Production Example 7: Preparation of Flea Nodule 6 fermented extract

The fermented extract was prepared in the same manner as in Preparation Example 2 except that the fermentation strain was changed to Phellinus linteus .

Production Example 8: Preparation of Flea Nodule 7 Fermented Extract

A fermented extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Grifola frondosa .

Production Example 9: Preparation of a complex extract

The combined extracts of the seedlings, yellow whites, daphniae and horseradishes obtained in Preparation Example 1 were mixed to prepare a complex extract.

Comparative Preparation Example: Preparation of Bacterial Fermentation Extract of Flea sp.

A fermentation extract was prepared in the same manner as in Preparation Example 2 except that only the fermentation strain was changed to Lactobacillus plantarum .

Examples and Comparative Examples

In order to verify the skin improvement effect of the obtained samples, the examples and comparative examples were set by mixing the samples as shown in Tables 1 and 2 below.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 9 10 11 12 13 14 Flea spot extract 60 - - - - - - - - - - - - - 1st fermentation extract - 60 - - - - - - 30 30 30 30 30 30 Second fermentation extract - - 60 - - - - - - - - - - - 3rd fermented extract - - - 60 - - - - - - - - - - Fourth fermented extract - - - - 60 - - - - - - - - - Fifth fermented extract - - - - - 60 - - - - - - - - 6th fermented extract - - - - - - 60 - - - - - - - No. 7 fermented extract - - - - - - - 60 - - - - - - Cloud mushroom extract - - - - - - - - 30 - - 15 - 10 Mushroom extract - - - - - - - - - 30 - - 15 10 Complex extract - - - - - - - - 30 15 15 10

[Content (parts by weight)] division Comparative Example One 2 3 4 5 6 7 Bacterial fermentation extract 60 - - - - - Cloud mushroom extract - 60 - 30 - 20 Mushroom extract - 60 - 30 20 Complex extract - - 60 30 30 20

Experimental Example 1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

Samples of Examples and Comparative Examples were suspended in purified water at concentrations of 0, 1, 5, 10, 20, 50, and 100 mg / L, respectively, and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, and 50 μL was added and cultured at 37 ° C and 5% CO ₂ for 48 hours. 100 L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example 2 Evaluation of Free Radical Scavenging Activity by DPPH Measurement

The extracts of Examples and Comparative Examples were suspended in purified water (concentration 10%) to evaluate free radical scavenging activity.

The DPPH assay is a method of measuring the absorbance at 540 nm of the extent to which the inhibitor decolorizes the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

Vitamin C was used as a control, and the same experiment was repeated 3 times for the accuracy of the experiment. The results are shown in Table 3 below.

[% of control] division DPPH radical scavenging activity (%) Example 1 111.7 Example 2 125.2 Example 3 123.5 Example 4 123.1 Example 5 122.4 Example 6 121.8 Example 7 121.6 Example 8 122.8 Example 9 127.8 Example 10 129.5 Example 11 129.6 Example 12 131.1 Example 13 133.3 Example 14 134.4 Comparative Example 1 102.1 Comparative Example 2 111.2 Comparative Example 3 108.5 Comparative Example 4 110.6 Comparative Example 5 118.1 Comparative Example 6 119.2 Comparative Example 7 119.5 Control group (vitamin C) 132.8

The higher the free radical scavenging activity, the better the antioxidative effect. The samples of Examples 1 to 14 containing the Flea sp. Extract had significantly higher free radical scavenging activity than the comparative examples 1 to 7.

In particular, the flea fermentation extracts (Examples 2 to 7) showed significantly improved free radical scavenging activity as compared to Example 1, suggesting that the antioxidative activity was significantly increased by fermentation.

Experimental Example 3: Evaluation of free radical scavenging activity by ABTS measurement

The extracts of Examples and Comparative Examples were suspended in purified water at a concentration of 100 μg / ml to evaluate free radical scavenging activity.

The ABTS method measures the degree of conversion of antioxidants to colorless ABTS at 732 nm by dissolving the blue-green radicals ABTS (2,2'-azinodi [3-ethylbenzthiazolne-6-sulfonic acid]).

Vitamin C was used as a control, and the same experiment was repeated three times for the accuracy of the experiment. The results are shown in Table 4 below.

[% of control] division ABTS radical scavenging activity (%) Example 1 120.7 Example 2 127.2 Example 3 123.5 Example 4 125.1 Example 5 122.4 Example 6 124.8 Example 7 123.6 Example 8 123.8 Example 9 127.8 Example 10 129.5 Example 11 129.6 Example 12 131.1 Example 13 133.3 Example 14 134.4 Comparative Example 1 111.8 Comparative Example 2 109.1 Comparative Example 3 111.2 Comparative Example 4 108.5 Comparative Example 5 110.6 Comparative Example 6 118.1 Comparative Example 7 119.2 Control group (vitamin C) 132.8

The higher the free radical scavenging activity, the better the antioxidative effect. The samples of Examples 1 to 14 containing the Flea sp. Extract had significantly higher free radical scavenging activity than the comparative examples 1 to 7.

In particular, the flea fermentation extracts (Examples 2 to 7) showed significantly improved free radical scavenging activity as compared to Example 1, suggesting that the antioxidative activity was significantly increased by fermentation.

Experimental Example 4: Measurement of total polyphenol compound content

Colorimetrically determined according to the Folin-Denis method in order to measure the amount of the total polyphenol compounds contained in Examples and Comparative Examples.

Each of the extracts of Examples and Comparative Examples was mixed with 5 mL of DW, mixed with 1 mL of folin reagent, and mixed with 1.5 mL of 20% Na ₂ CO ₃ solution. The mixture was allowed to stand at room temperature for 2 hours and absorbance was measured at 765 nm.

Total polyphenol contents were expressed as GAE (gallic acid equivalents). Polyphenols contained in the extracts were quantitated using a standard curves of previously prepared gallic acid.

division Total polyphenol chemistry (μg GAE / 1 mg) Example 1 53.0 Example 2 90.2 Example 3 70.1 Example 4 75.1 Example 5 75.3 Example 6 71.3 Example 7 72.3 Example 8 75.2 Example 9 103.1 Example 10 115.3 Example 11 114.2 Example 12 118.2 Example 13 118.5 Example 14 124.1 Comparative Example 1 61.1 Comparative Example 2 61.3 Comparative Example 3 60.8 Comparative Example 4 65.1 Comparative Example 5 65.3 Comparative Example 6 66.5 Comparative Example 7 69.1

Since polyphenol is an antioxidant with antioxidant effect, it can be evaluated that the antioxidative effect is superior as the total polyphenol content of the extract increases.

The samples of Examples 1 to 14, which contained flea extracts, contained more polyphenols than Comparative Examples 1 to 7.

Particularly, the content of polyphenol in the flea fermentation extracts (Examples 2 to 7) was significantly increased as compared with Example 1, suggesting that the antioxidative effect was increased by fermentation.

Experimental Example 5: Evaluation of melanin production

In order to confirm the whitening effect of the examples and the comparative examples, the melanin contents assay was used to evaluate melanogenesis inhibition by the samples of the examples and comparative examples (Eisinger M et al, 1982; Siegrist W and Eberle AN, 1986). The samples of the examples and comparative examples were each suspended in purified water at a concentration of 50 mg / L and the amount of melanin produced was evaluated.

B16F10 cells were inoculated in a 60 mm culture dish at a concentration of ^1.5 × 10 ⁵ cells / well and cultured for 24 hours. Α-MSH (Sigma-Aldrich, St. Louis, MO, USA), a hormone that promotes melanin formation, and samples of the Examples and Comparative Examples were added to the medium at a concentration of 200 μg / mL (Virador VM et al., 1999).

After 48 hours, the cells were harvested and washed once with PBS. Then, 120 μL of 1 N NaOH lysis buffer was added and the cells were lysed by heating at 95 ° C. for 10 minutes. Absorbance was measured at a wavelength of 450 nm using a microplate reader and arbutin at a concentration of 200 μg / mL was used as a control (Table 6).

Particularly, Example 1 measured melanin production rate by concentration (Fig. 1).

division Melanin production rate (%) Example 1 41.2 Example 2 30.1 Example 3 32.2 Example 4 34.3 Example 5 33.1 Example 6 35.1 Example 7 36.1 Example 8 34.1 Example 9 29.1 Example 10 28.4 Example 11 28.5 Example 12 23.6 Example 13 23.4 Example 14 22.4 Comparative Example 1 78.6 Comparative Example 2 76.2 Comparative Example 3 74.2 Comparative Example 4 77.2 Comparative Example 5 74.1 Comparative Example 6 72.1 Comparative Example 7 66.2 Positive control group 68.2

Suppression of melanin production can be evaluated as an excellent whitening effect. The samples of Examples 1 to 14, in which the flea bite extracts contained, inhibited melanogenesis to an equivalent or higher level compared to Comparative Examples 1 to 7.

In particular, the fermented extract of fleas (Examples 2 to 7) showed an improvement in the melanin inhibitory effect as compared with Example 1, suggesting that the whitening effect was significantly increased by fermentation.

Experimental Example 6: Evaluation of improvement effect of skin tone

The effect of improving the skin color of the cosmetic composition containing the samples obtained in Examples and Comparative Examples was evaluated. Samples of Examples and Comparative Examples were formulated into cream of the same composition, and samples of Examples 1 to 14 and Comparative Examples 1 to 7 were different.

The skin color improvement degree was evaluated by applying the creams of Examples and Comparative Examples formulated to 210 selected from 20 to 60 year old women.

The 210 creams were divided into 21 groups of 10, and the formulated cream was applied to the entire face for 2 weeks at a temperature of 24 to 26 ° C and a humidity of 75% for 12 weeks, and skin color improvement The effects were evaluated.

division Significant improvement (persons) Some improvement (persons) No improvement (persons) Example 1 3 2 5 Example 2 6 3 One Example 3 4 2 4 Example 4 4 2 4 Example 5 5 3 2 Example 6 4 2 4 Example 7 5 2 3 Example 8 4 3 3 Example 9 7 2 One Example 10 7 One 2 Example 11 7 One 2 Example 12 8 One One Example 13 8 2 - Example 14 9 One - Comparative Example 1 2 2 6 Comparative Example 2 2 One 7 Comparative Example 3 3 One 6 Comparative Example 4 2 One 7 Comparative Example 5 2 2 6 Comparative Example 6 One 2 7 Comparative Example 7 2 2 6

Referring to Table 7, it was evaluated that the samples of Examples 1 to 14 were significantly superior in the effect of improving skin color as compared with the samples of Comparative Examples 1 to 7.

In particular, the flea-perilla fermentation extracts (Examples 2 to 7) significantly improved the skin color improving effect as compared with Example 1.

Experimental Example 7: Evaluation of skin elasticity improvement

The skin elasticity improving effect of the cosmetic composition containing the samples obtained in Examples and Comparative Examples was evaluated. Each of the above samples was formulated into cream of the same composition, and samples of Examples 1 to 14 and Comparative Examples 1 to 7 were different.

Healthy women aged 20 years or older (average age 37 years old) were divided into groups in the conditions of temperature of 24 to 26 캜 and humidity of 75%, and the creams of the examples and the comparative examples were respectively applied.

The formulated cream was applied for 12 weeks twice a day (morning and evening) around the eyes, and skin elasticity was measured using a skin elasticity analyzer (Cutometer MPA580, Conrage + Khazaka, Germany). The results of the test are described in the R8 (R8 (12 weeks) -R8 (0 weeks)) value of the cutometer MPA580 and the R8 value indicates the properties of the skin viscoelasticity.

division Skin elasticity improvement effect Example 1 0.65 Example 2 0.77 Example 3 0.72 Example 4 0.73 Example 5 0.72 Example 6 0.71 Example 7 0.73 Example 8 0.72 Example 9 0.78 Example 10 0.79 Example 11 0.80 Example 12 0.92 Example 13 0.92 Example 14 0.94 Comparative Example 1 0.42 Comparative Example 2 0.64 Comparative Example 3 0.54 Comparative Example 4 0.54 Comparative Example 5 0.55 Comparative Example 6 0.62 Comparative Example 7 0.65 Positive control (vitamin C) 0.92 Negative control (no treatment) 0.31

The samples of Examples 1 to 14 were remarkably superior in the skin elasticity improving effect as compared with Comparative Examples 1 to 7.

In particular, the flea fermentation extract (Examples 2 to 7) significantly improved skin elasticity improvement effect as compared with Example 1.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

The flea contains a locust extract as an active ingredient,
The flea sp. Extract is a fermented extract prepared by inoculating a fermentation strain,
Antioxidant, whitening and skin elasticity. delete The method according to claim 1,
The fermentation strains are selected from the group consisting of Hericium erinaceum , Pleurotusus ferulae , Sparassis crispa , Ganoderma lucidum , Flammulina velutipes , Phellinus linteus and mushroom Grifola frondosa ). ≪ / RTI > The method according to claim 1,
A mushroom extract, a mushroom extract, a mushroom extract, and a shiitake mushroom extract. The method according to claim 1,
Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Alaska pollack extract, Extract of Leaf Lobster, Extract of Ganoderma lucidum Wherein the composition further comprises at least one member selected from the group consisting of gold extract, Angelica keiskei koidz. delete 6. The method according to any one of claims 1 to 5,
Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohols having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol A cosmetic composition which is extracted with at least one solvent. 6. The method according to any one of claims 1 to 5,
The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is at least one selected from the group consisting of a softening agent, a nutritional lotion, a moisturizing cream, a nutritional cream, a massage cream, a nutrient lotion, an essence, an ampoule, a gel, an eye cream, an oil, a foundation, a cleansing cream, a cleansing foam, a cleansing water, a shampoo, Spray, and powder. ≪ RTI ID = 0.0 > 18. < / RTI >

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