KR101469807B1 - Cosmetic compositions containing fermented extracts of coriolus versicolor mushroom - Google Patents

Abstract

The present invention relates to a raw material composition for cosmetics which is useful for antioxidation of Fungus mushroom fermentation extract using Lactobacillus strain, and it is confirmed that the extract has better activity after fermentation than ethanol extract. Fungus mushroom fermented with Lactobacillus acidophilus clears free radicals and free radicals, and Fungus mushroom fermented with Lactobacillus plantarum has the effect of eliminating free radicals .

Description

COSMETIC COMPOSITIONS CONTAINING FERMENTED EXTRACTS OF CORIOLUS VERSICOLOR MUSHROOM [0002]

The present invention relates to an antioxidant activity of a fermented mushroom fermentation extract using Lactobacillus strain. In addition, the above extract is excellent in free radical scavenging activity and oxygen scavenging ability, and relates to the use of gastric extract having this effect as an antioxidant.

Oxygen is needed to breathe but it is directly or indirectly involved in causing various diseases such as aging, arthritis, and cancer. This refers to activated oxygen species, which are free radicals produced by cellular immune responses, intracellular energy production reactions, ultraviolet light, stress, and bacteria. It is also known that when it is produced in the body, it destroys cells or causes various problems such as wrinkles, skin cancer, atopic dermatitis and acne. Antioxidants are widely distributed in copper and plants. Many phenolic compounds, flavonoids, tocopherols, and vitamin C in fruits and vegetables prevent or retard oxidation of lipids and prevent aging.

Korea Patent No. 10-0775133

It is an object of the present invention to provide a fermented extract of chestnut mushroom having an antioxidant effect superior to the ethanol extract of chestnut mushroom.

The object of the present invention can be attained by fermenting Mushroom mushroom with Lactobacillus strain.

Fungus mushroom is extracted at room temperature using water and ethanol as a mixed solvent. Lactobacillus strains are mixed at a suitable ratio with the mushroom extract and cultured at 37 ° C.

The fermented extract has the better antioxidative effect when the Lactobacillus strain is mixed at the best ratio than the ethanol extract.

1 is a loose-fill (Lactobacillus FIG fingering mushroom non fermented with Lactobacillus know of the present invention acidophilus ) fermentation extracts.
FIG. 2 is a graph showing free radical and reactive oxygen scavenging effect according to the concentration of the fermented extract of L. bifuscolius Lactobacillus plantarum according to the present invention.
3 is loose-fill (Lactobacillus FIG fingering mushroom non fermented with Lactobacillus know of the present invention acidophilus ) fermentation extracts.
FIG. 4 is a graph showing the cytotoxicity according to the concentration of the fermented extract of Lactobacillus plantarum and the non-fermented fungus mushroom of the present invention.

The fingi mushroom used in the present invention is a mycelium of mushroom and mushroom ( Coriolus versicolor (LINNE) FR.). Efficacy in the treatment of lung diseases, hepatitis B, delayed hepatitis, chronic active hepatitis and hepatocarcinoma. The main component of the fungus mushroom is polysaccharide, and the substance that constitutes the cell wall of fungus mushroom is glucan. Glucan is a major component of antitumor drugs and is a high-priced medicine.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples.

Example

Example 1. Extraction method using 70% ethanol

Fenugami mushroom and 70% ethanol were mixed at a weight ratio of 1:10 and extracted with shaking at room temperature for 24 hours.

Example 2. Lactobacillus < RTI ID = 0.0 > acidophilus )

20% ethanol extract and 20% Lactobacillus acidophilus (OD ₆₀₀ = 0.4) were mixed and extracted with shaking at 37 ° C for 19 hours.

Example 3. Lactobacillus ( Lactobacillus) Fermentation Extraction Method Using Plantarum Strains (1)

20% ethanol extract and 0.6% Lactobacillus plantarum (OD ₆₀₀ = 0.1) were mixed and shaken at 37 ° C for 24 hours.

Example 4. Lactobacillus ( Lactobacillus) fermentation extraction method (2) using a plantarum) strains

10% ethanol extract and 20% Lactobacillus plantarum (OD ₆₀₀ = 0.4) were mixed and shaken at 37 ° C for 24 hours.

Test Example

Test Example 1 Reduction activity by free radical scavenging method by DPPH

(DPPH, 1,1-diphenyl-2-picryl hydrazyl, Sigma, USA) is a chemically stabilized water-soluble material with a free radical and has a maximum absorbance at around 520 nm And if it meets a substance with antioxidant activity, the radical (DPPH) disappears and the color changes while giving electrons. Chemically stable DPPH can be used to analyze the antioxidative effects of various types of antioxidant extracts, beverages, oils, and pure phenolic compounds. This experiment is an experiment to examine the activity of scavenging by radicals after initiating oxidation first with DPPH. The ethanol extract of Example 1 and the fermented extracts of Examples 2, 3 and 4 were added to 1 mL of 0.2 mM DPPH solution in which DPPH was dissolved in methanol. After incubation at room temperature for 10 minutes, the absorbance was measured at 525 nm using an ELISA reader (Plus384, Molecular Device, USA). In the control group, methanol is added instead of the sample solution and the DPPH solution to obtain a correction value. The free radical scavenging rate was calculated according to the following equation.

(%) = (Absorbance of control group - absorbance of test group) / absorbance of control group x 100

The control group was 6-hydroxy-2,5,6,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma, USA). Trolox is a vitamin E analogue with excellent antioxidant activity. Samples with similar or higher values to trolox were considered active at 50% activity

Fungus mushroom fermented with Lactobacillus acidophilus was about 40% more active than ethanol extract, and the results are shown in Fig. The extracts of the fungus mushroom fermented under the conditions of Example 3 were similar to those of the ethanol extract, and the fungus mushroom fermented under the condition of Example 4 was about 10% more active than the ethanol extract. The results are shown in Fig.

Test Example 2 SOD-like activity measurement

Reactive Oxygen Species The SOD activity was measured by measuring the absorbance change of NBT (Nitrobluetetrazolium) by reactive oxygen species using the active oxygen generating system by xanthine oxidase enzyme reaction do. _{0.05mM Na 2 CO 3 (Yakuri Pure} Chemicals Co., Ltd, Japan) 1.2mL, 3mM xanthine (Sigma, USA) 0.05mL, 3mM EDTA (Sigma, USA) 0.05mL, BSA (Sigma, USA) 0.05mL, 0.75 0.05 mL of mM NBT (Sigma, USA) and 0.05 mL of each sample (the same as in Test Example 1) were added, mixed well and allowed to stand at 25 DEG C for 10 minutes. The reaction was stopped by the addition of 0.05 mL of 6 mM CuCl ₂ (Daejung Chemicals & Metals Co., LTD, Korea) in 0.05 mL of xanthine oxidase (Sigma, USA) Device, USA) at 560 nm. In the control group, purified water is added instead of the sample, and purified water is added instead of xanthine oxidase to obtain a color correction value. The erasure rate was calculated in the same manner as the equation used for the DPPH free radical scavenging rate. The control group was vitamin C (L-ascorbic acid, Sigma, USA). The activity was rated at 50%, and if similar or higher values were considered, the activity was considered high.

The fungus extract fermented under the conditions of Example 2 was about 17% more active than the ethanol extract, and the results are shown in FIG. Uncooked mushroom fermented under the condition of Example 3 was about 27% more active than ethanol extract, but the fermented mushroom fermented in Example 4 was less active than ethanol extract. The results are shown in Fig.

Test Example 3. Cytotoxicity measurement

Cells are stained with 3- [4,5-dimethyltjiazol-2-yl] -2,5-diphenyl tetrazolium bromide (MTT, Amresco) HaCaT cells are seeded at 6 × 10 ³ cells / well on a 96-well plate and cultured for 24 hours. Samples are treated at 0.1, 0.5, 1, 2% and cultured for 72 hours. MTT (5 mg / mL) was treated for 4 hours and absorbance was measured at 570 nm with an ELISA reader (Plus 384, Molecular Device, USA). The cell survival rate was considered to be toxic if 80% or more and less than 80%.

The ethanol extracts and the unmixed mushrooms fermented under the conditions of Example 2 and 3 were not toxic up to 1%, but the mushrooms extracted from the fermented mushrooms under the conditions of Example 4 were toxic at all concentrations. The results are shown in Fig. 3 and Fig.

Fungus mushroom is fermented as Lactobacillus strain, and it has better antioxidative effect and can be applied to cosmetic raw materials. The extract of Fungus mushroom has free radical scavenging effect and active oxygen scavenging effect and can exhibit anti-aging and antioxidant effect and can be applied to products showing this function.

Claims (4)

A cosmetic composition comprising a fermented extract by Lactobacillus strain of Mushroom mushroom.
delete The cosmetic composition according to claim 1, wherein the Lactobacillus strain is at least one selected from Lactobacillus acidophilus and Lactobacillus plantarum . [Claim 4] The cosmetic composition according to claim 3, wherein the fermented extract is fermented and extracted with fungus mushroom and Lactobacillus acidophilus (OD ₆₀₀ = 0.4) at an experimental ratio of 20%.

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