KR102131774B1 - Cosmetic Composition Containing Extract of Trichaptum Abietinum for Improving Skin Wrinkle - Google Patents

Abstract

The present invention is a pine tree ( Trichaptum abietinum ) It relates to a cosmetic composition for improving or anti-wrinkle skin containing a fermented extract as an active ingredient, specifically, containing 0. 0001 to 30.0% by weight relative to the total weight of the cosmetic composition as an active ingredient of the ethanol extract By providing a cosmetic composition having an excellent effect on antioxidant and skin wrinkle improvement.

Description

A cosmetic composition for improving skin wrinkles containing an extract of oleander mushroom {Cosmetic Composition Containing Extract of Trichaptum Abietinum for Improving Skin Wrinkle}

The present invention is a mushroom ( Trichaptum) abietinum ) It relates to a cosmetic composition for improving skin wrinkles containing a fermented extract as an active ingredient.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into intrinsic aging and photo-aging, and research on this has been actively conducted. UV rays, stress, disease states, environmental factors, wounds, and free radicals can be activated as they age, and when these conditions become severe, they destroy the antioxidant defense network in the living body and damage cells and tissues, resulting in adult diseases and It promotes aging. More specifically, skin, cells and tissues are destroyed by oxidation of lipids, proteins, polysaccharides, and nucleic acids, which are the main constituents of the skin, and eventually skin aging occurs.

Protein oxidation disrupts severe hyper-inflammatory reactions and elasticity of the skin by cutting the connective tissue of the skin, collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin. If it gets worse, mutations in DNA can lead to mutations, cancer, and reduced immune function. Therefore, free radicals generated during metabolic processes in the body or ultraviolet radiation, free radicals mediated by an inflammatory reaction are eliminated to protect the cell membrane, and already damaged cells must be regenerated by active metabolism to proliferate the cells. The skin recovers quickly and can maintain healthy skin.

As a feature in aged skin, elastin fibers in the skin form cross-links inside the dermis along with collagen fibers and are involved in skin elasticity. With age, the skin histologically shows a deficiency and agglutination of elastin fibers, a decrease in collagen fibers, an increase in inflammatory cell infiltration and a dramatic increase in the activity of elastin, the elastin-degrading enzyme. Therefore, the elasticity of the skin is rapidly reduced by the action of elastase. As a solution that can reduce skin aging by inhibiting elastase, studies on natural products having elastin-degrading enzyme inhibitory activity have been continued to date.

In addition, not only free radicals, but also an enzyme called matrix metalloproteinase (MMP), which is a collagen degrading enzyme, is involved in aging. In other words, the synthesis and decomposition of extracellular matrix such as collagen in vivo is appropriately controlled, but as aging progresses, collagen synthesis decreases, and the expression of matrix metal proteolytic enzyme (MMP), an enzyme that degrades collagen, is promoted to the skin. Its elasticity is reduced and wrinkles are formed. In addition, such degrading enzymes are also activated by ultraviolet irradiation. Therefore, there is a need to develop a substance capable of controlling or inhibiting the activity of MMP expression in which activation is induced in cells.

Oxygen, along with water, is an indispensable substance in our lives, but once it enters our body, it is transformed into highly active oxygen by various causes, oxidizing vascular cells. Today, about 90% of the causes of disease are attributed to this free radical. The human body is composed of about 60 trillion cells, and the cell membrane is formed of the cortex, so free radicals are easily connected. When an oxidation reaction occurs, unsaturated fatty acids are converted to lipid peroxide. This lipid peroxide damages gene DNA, RNA, protein, cell membrane and cell structure, causing cancer or several adult diseases, ultimately causing aging. It is the antioxidant that removes or neutralizes these free radicals to prevent the cells from oxidizing. In general, when peroxidation occurs due to free radicals of lipids, which are components of skin cells, the structure of the cell membrane is modified, resulting in cell permeability, fluidity, inactivation of membrane enzymes and transport proteins, cleavage of protein chains, DNA, RNA damage, etc. It is known that skin cells age. For this reason, efforts to remove free radicals continued, and as a result, many sulfated substances were developed. Materials that have been known to have antioxidant activity so far include ascorbic acid, beta-carotene, dibutylhydroxytoluene (BHT), and D-alpha-tocopherol. . However, since these substances are used as synthetic substances, there is a problem in skin safety when used for a long time or when used in a large amount, or when it is contained in a formulation in cosmetics, there is a problem in stability, which is limited in its use. to be. Therefore, many researchers are trying to solve these problems.

Korean Patent Publication No. 2018-0052151 discloses a cosmetic composition containing an active ingredient of a mushroom extract of zinnia, while the cosmetic composition has an effect of promoting collagen production and suppressing elastane activity. In addition, Korean Patent Registration No. 10-1456052 discloses a cosmetic composition having a skin wrinkle improvement effect, including truffles.

The present inventors have search results for a variety of skin bioactive natural plant ingredients to improve the aging, clothes brush mushrooms from natural plant extracts (Trichaptum abietinum ) The present invention was completed by confirming that the fermented extract can effectively improve skin by showing antioxidant, elastase inhibitory activity, collagen synthesis promoting effect, and MMP-1 production inhibitory effect.

Object of the clothes brush is mushroom (Trichaptum abietinum ) It is achieved by a cosmetic composition for improving or anti-wrinkle skin containing a fermented extract.

Preferably, the above mushroom ( Trichaptum abietinum ) Ferment extract is characterized in that it is contained in 0.0001 ~ 30.0% (w / w) relative to the total weight of the cosmetic composition.

In addition, preferably, the chamois mushroom ( Trichaptum abietinum ) extract promotes collagen biosynthesis and is characterized by inhibiting the activity of MMP-1.

In addition, preferably, the fermented extract of oleander mushroom may be fermented with a solvent extract of oleander mushroom with Lactobacillus acidophilus or Bifidobacterium lactis .

In addition, preferably, the fermented extract of oleander mushroom may be fermented with a mixed strain in which the solvent extract of oleander mushroom is mixed with Lactobacillus acidophilus and Bifidobacterium lactis in the same weight ratio. have.

In addition, preferably, the solrush mushroom solvent extract may be extracted with an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, and butyl acetate.

The present invention is a mushroom ( Trichaptum) abietinum ) It relates to a cosmetic composition for improving heavy metal, fine dust adsorption and skin wrinkles containing fermented extract as an active ingredient. Trichaptum , an active ingredient contained in the cosmetic composition of the present invention abietinum ) The fermented extract has excellent collagen biosynthesis and MMP-1 production inhibitory effect, so it has excellent wrinkle improvement and anti-aging effects.

All technical terms used in the present invention, unless defined otherwise, have the following definitions and conform to the meaning as commonly understood by those skilled in the art in the relevant field of the present invention. In addition, although a preferred method or sample is described herein, similar or equivalent ones are included in the scope of the present invention.

The term “about” means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, for reference amount, level, value, number, frequency, percent, dimension, size, amount, weight or length It means a quantity, level, value, number, frequency, percentage, dimension, size, quantity, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, the terms "comprises" and "comprising", unless the context requires otherwise, include the steps or components presented, or a group of steps or components, but any other steps or components, or It should be understood that it implies that a group of steps or components is not excluded.

The present invention is a mushroom ( Trichaptum abietinum ) relates to a cosmetic composition comprising a fermented extract as an active ingredient, and relates to a cosmetic composition having antioxidant and skin wrinkle improvement effects.

A clothes brush used in the present invention mushrooms (Trichaptum abietinum ) is a mushroom of the genus oleander mushroom of the genus genus genus, and is distributed in all parts of the world, including Korea, East Asia, and Europe. Oyster mushrooms are attached to dead conifers and grow in bunches by semi-adhesion. The surface of the mushroom cap is gray or white, and the tissue of the fruiting body is very thin and has a pink or light purple fruit layer. It is a natural product with high use value and research value because it is not used for medicinal and edible purposes due to unknown food poisoning.

Trichaptum of the present invention abietinum ) extract may be prepared according to conventional methods known in the art, that is, under conditions of conventional temperature and pressure, using a conventional solvent, but preferably (a) water, 1-4 carbon atoms. Anhydrous or hydrous lower alcohols (eg methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane And a solvent extraction method using a solvent selected from the group consisting of mixtures thereof (b) extraction using a supercritical extraction method with reduced pressure and high temperature with carbon dioxide or (c) an ultrasonic extraction method. In the present invention, a solvent extraction method using an extraction solvent is preferably used. Tricotum abietinum extract in the present invention is a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), propylene glycol, 1, 3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and one or more extraction solvents selected from the group consisting of mixtures thereof. Is obtained using ethanol, 70% (v/v) ethanol or water, most preferably 70% (v/v) ethanol. On the other hand, it is apparent to those skilled in the art that the extract of the present invention can be obtained by using the extract of Trichaptum abietinum in addition to the above-mentioned extraction solvent, as well as other extraction solvents.

In addition, the clothes of the present invention ( Trichaptum) abietinum ) extract includes not only extracts by the above-described extraction solvent, but also extracts that have been subjected to conventional purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) Active fractions obtained through various purification and extraction methods, such as separation by sex) or extraction by fermentation products using natural or various microorganisms, are also included in the extract of the present invention.

Clothes brush according to the invention mushrooms (Trichaptum abietinum ) extract contains fermented extracts, Trichaptum abietinum ) Ferment extract can be prepared as follows. Trichaptum abietinum ) After crushing the outpost finely to about 100 to 500 mesh, 1 to 50 g/L of a conventional microbial culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu/L. The culture temperature is cultured under normal microorganism culture conditions of 30~37℃. The pH is 5-7, and cultured for about 5 to 10 days under aerobic or normal anaerobic conditions. It can then be obtained through aging and filtration.

According to an embodiment of the present invention, the fermented extract of oxen mushroom can be prepared by fermenting the solvent extract of oxen mushroom with microorganisms. Preferably, the microorganism may be Lactobacillus acidophilus or Bifidobacterium lactis , and more preferably Lactobacillus acidophilus and Bifidobacterium lactis . It can be used by mixing in the same weight.

According to the present invention, the ox mushroom ( Trichaptum abietinum) fermentation extract is contained 0.0001 ~ 30.0% by weight based on the total weight of the cosmetic composition, and more preferably, the clothes brush mushroom (Trichaptum abietinum ) Ferment extract is characterized in that it contains 0.01 to 10% by weight relative to the total weight of the cosmetic composition. When the content of the extract is less than 0.001% by weight, the skin improvement effect does not appear, and when it exceeds 30.0% by weight, the degree of improvement of the skin improvement effect on the increase in content is negligible, and there is a problem in the safety and stability of the formulation. Not even enemies

Therefore, Trichaptum with various functions abietinum ) The cosmetic composition of the present invention comprising a fermented extract is characterized by having excellent antioxidant effect, elastase inhibitory activity, collagen biosynthesis effect, and MMP-1 production inhibitory effect.

The components included in the cosmetic composition of the present invention, as active ingredients, may include ingredients commonly used in cosmetic compositions in addition to the active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances Adjuvants, and carriers. The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but may be formulated, but is not limited thereto. More specifically, it can be prepared in the form of flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier components. Can be.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether as a carrier component Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be wiped, peeled off, or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, a cleansing towel and a cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotions, cleansing water and cleansing gels, but are not limited thereto. Meanwhile, the cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention. The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of uses can be varied according to the user's skin condition or taste.

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to illustrate the present invention in more detail, and according to the gist of the present invention, the scope of the present invention is not limited by these examples.

Preparation Example 1- Trichaptum abietinum ) solvent extract preparation

After washing the outsole of oxen mushrooms with clean water and drying it sufficiently, it was cut to a certain size and refluxed 3 times for 5 hours with 70% (V/V) ethanol aqueous solution, cooled, and filtered with Whatman #4 filter paper. . The filtered extract was concentrated under reduced pressure at 50°C or lower and freeze-dried. The dried extract was diluted to a constant concentration and used in the following experiment.

Preparation Example 2- Trichaptum abietinum ) fermentation extract preparation

The extract of oxen mushroom obtained from Preparation Example 1 was added to the culture solution to be 10 g/1L, and dipotassium phosphate was added as a glucose and pH regulator as a carbon nutrient. After adding 50,000 cfu/L of Lactobacillus acidophilus per culture medium (KTCT 3140), the cells were cultured at 37°C for 7 days under conditions of pH 5-7. After incubation, the culture medium was filtered using a 0.25 μM filter, and the filtrate was concentrated to prepare 1.0 kg of fermentation extract.

Preparation Example 3- Trichaptum abietinum ) fermentation extract preparation

The extract of oxen mushroom obtained from Preparation Example 1 was added to the culture solution to be 10 g/1L, and dipotassium phosphate was added as a glucose and pH regulator as a carbon nutrient. After adding 50,000 cfu/L of Bifidobacterium lactis (5854) per culture medium, the cells were cultured at 37°C for 7 days under conditions of pH 5-7. After incubation, the culture medium was filtered using a 0.25 μM filter, and the filtrate was concentrated to prepare 1.0 kg of fermentation extract.

Preparation Example 4- Trichaptum abietinum ) fermentation extract preparation

The extract of oxen mushroom obtained from Preparation Example 1 was added to the culture solution to be 10 g/1L, and dipotassium phosphate was added as a glucose and pH regulator as a carbon nutrient. After adding Lactobacillus acidophilus (KTCT 3140) and Bifidobacterium lactis (5854) per culture solution at the same rate, 25,000 cfu/L were added, followed by incubation at 37°C for 7 days at 37°C under pH 5-7. After incubation, the culture medium was filtered using a 0.25 μM filter, and the filtrate was concentrated to prepare 1.0 kg of fermentation extract.

Experimental Example 1- DPPH radical scavenging activity measurement

In order to measure the antioxidant effect of Preparation Example 1, L-Ascorbic acid was compared as a comparative sample under laboratory conditions to measure antioxidant activity using the DPPH method. The DPPH method measures antioxidant activity by reducing power using a free radical called DPPH(2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl) free radical. The free radical scavenging rate is measured at a wavelength of 560 nm by comparing the degree of decrease in absorbance due to the reduction of DPPH by the test substance with the absorbance of the blank test solution.

As a reagent used, 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW=618.76) is dissolved in methanol as a 0.2 mM solution to make 100 ml. As a measurement method, 96-well 0.15 ml of 0.2 mM DPPH solution and 0.15 ml of sample solution are added to the plate, stirred quickly, and incubated for 10 minutes at 25° C. Absorbance St at 560 nm is then measured. In the blank test, absorbance Bt was measured by using distilled water instead of the sample solution in the same manner as above. The blank of the sample solution is operated in the same manner using methanol instead of the 0.2 mM DPPH solution to measure the absorbance Bo.

As a result of experimenting with various concentrations to calculate the SC ₅₀ value, the DPPH radical scavenging ability was confirmed through the following equation 1, and the results are shown in Table 1. L-ascorbic acid was used as a comparison group.

[Equation 1]

St: absorbance at 560 nm after free radical scavenging of sample solution

Bt: Absorbance at 560 nm after free radical scavenging of the blank test solution

So: Absorbance at 560 nm before reaction when free radicals are not added to the sample solution

Bo: Absorbance at 560nm before reaction when free radicals are not added to the blank test solution

Sample name SC ₅₀ (ppm) Preparation Example 1 135.03 ± 2.18 Preparation Example 2 108.57 ± 3.47 Preparation Example 3 115.12 ± 3.52 Preparation Example 4 98.85 ± 2.73 L-ascorbic acid 35.18 ± 3.15

Experimental Example 2- Elastase inhibitory activity measurement

Elastase is an enzyme that breaks down elastin, an important substrate for maintaining skin elasticity in the dermis. In addition, elastase is a non-specific hydrolase that can degrade collagen, another important matrix protein. Therefore, an elastase inhibitor has an effect of improving skin wrinkles, and ursolic acid is used as an elastase inhibitor. Elastase is known as an enzyme that is the main cause of wrinkle formation by breaking down the structure of the skin's dermal structure by breaking down elastin, an insoluble elastic fiber protein of animal connective tissue. In the dermal tissue of the skin, collagen and elastin related to the elasticity of the skin form a mesh structure. As the mesh structure is broken, that is, elastin is decomposed by elastase, the skin sags and wrinkles, resulting in endogenous skin aging. do. Therefore, it is possible to suppress skin aging by lowering the activity of elastin, an elastin degrading enzyme, which is one of the main causes of skin aging. Using N-succinyl-(L-Ala)3-p-nitroanilide, S4760, Sigma) as a substrate, the amount of p-nitroanilide produced from the substrate at 37°C for 20 minutes was measured at 445 nm. That is, each test solution was prepared to have a constant concentration, and 500 μL of each was taken in a test tube, and 500 μL of porcine pancreas elastase (E1250, Sigma) 2.5 U/mL) dissolved in 2 mM tris-HCl buffer (pH8.6) was added to the substrate. It was measured by adding N-succinyl-(L-Ala)3-p-nitroanilide (500μg/mL) dissolved in 50mM tris-HCl buffer (pH8.6) for 20 minutes. Elastase inhibitory activity was calculated according to the following Equation 2 as the absorbance reduction rate of the sample solution with and without additives.

[Equation 2]

Sample name Treatment concentration (ppm) Elastase Inhibition rate ( % ) Preparation Example 1 100 9.08 ± 3.10 1000 22.48 ± 2.81 Preparation Example 2 100 14.47 ± 1.98 1000 30.12 ± 2.17 Preparation Example 3 100 16.87 ± 2.84 1000 34.47 ± 2.46 Preparation Example 4 100 27.21 ± 2.15 1000 41.11 ± 2.07

Experimental Example 3- Collagen biosynthesis measurement result using ELISA kit

DMEM (Dulbecco's) containing 10% FBS, Penicillin (50U/ml), and Streptomycin (50/ml) in a cell incubator that maintains constant humidity in human skin fibroblasts at 37°C and 5% CO ₂ incubator Cultured with Modified Eagle's Medium, Gibco, USA), dispensed 500 μl into a 24 well plate at 1×10 ⁵ cells/ml and then incubated for 24 hours. The quantification of collagen type I in fibroblasts was carried out by an immunosorbent assay (ELISA) using'Procollagen Type I c-peptide EIAkit (TaKaRa, japan)', 48 hours for analysis. The cell culture supernatant cultured above was carefully collected. First, 100 μl of each culture supernatant cultured for 48 hours was added to a strip coated with'Procollagen Type I cpeptide, and the mixture was reacted at 37° C. for 2 hours. After the reaction, washing was performed three times using a washing buffer to remove unreacted substances, and then 100 ul of a blocking buffer was added thereto, followed by reacting at 37° C. for 2 hours. After the reaction, the unreacted material is removed by washing 3 times using a washing buffer, and a solution in which POD is conjugated to another antibody binding to'Procollagen Type I c-peptide' (Antiobody- POD conjugate solution) was added in 100 ul and reacted at 37°C for 2 hours. After the reaction, wash 3 times using a washing buffer to remove unreacted substances, add an equal amount of substrate solution, react for 30 minutes, then add a stop solution (1N H2SO4) to perform the experiment. Ended. After the experiment, the absorbed strip was measured at 450 nm, and the amount of newly synthesized collagen was measured as the amount of PICP compared to the collagen standard, and the synthesis effect of collagen is shown in Table 3.

Sample name Treatment concentration (ppm) Collagen synthesis amount
(ng/2*10 ⁴ ) Preparation Example 1 100 73 ± 1.54 200 91 ± 3.74 Preparation Example 2 100 79 ± 2.15 200 97 ± 3.21 Preparation Example 3 100 76 ± 2.17 200 92 ± 2.87 Preparation Example 4 100 86 ± 2.07 200 104 ± 2.48

Experimental Example 4- MMP-1 production inhibitory effect

Experimental Example 4 is to measure whether or not the effect of inhibiting the production of MMP-1, which is a collagen degrading enzyme, by using the extract of oleander mushroom obtained in Preparation Example 1, has an effect of inhibiting the production of MMP-1. The effect of inhibiting the production of MMP-1 was measured using a known substance, TGF-β (10 ng/ml, Roche, United States) as a comparison group. Human normal skin cells, fibroblasts (Korea Cell Line Bank, Korea), were inoculated into 48-well microplates (Nunc, Denmark) to be 1×10 ⁶ cells per well, and conditions of DMEM medium (Sigma, United States) and 37° C. After incubation for 24 hours, serum-free DMEM medium added at a final concentration of 100 μg/ml of the extract of oxen mushroom of Preparation Example 1 and a control-free culture for 48 hours in serum-free DMEM medium not containing oxen mushroom extract as a control Did. After incubation, the supernatant of each well was collected to measure the amount of newly synthesized MMP-1 (ng/ml) using the MMP-1 analysis kit (Amersham, United States), and MMP-1 was generated according to Equation 3 below. The inhibition rate (%) was calculated, and the results are shown in Table 4.

[Equation 3]

Sample name Treatment concentration (nM) MMP -1 production inhibition rate ( % ) Preparation Example 1 100 10.27 ± 2.18 200 18.74 ± 3.09 Preparation Example 2 100 18.71 ± 2.97 200 27.25 ± 3.36 Preparation Example 3 100 15.87 ± 2.49 200 29.18 ± 2.83 Preparation Example 4 100 25.07 ± 3.10 200 44.10 ± 2.54

Through the above experimental examples, it was possible to confirm the efficacy effects of the preparation examples, and efficacy verification was re-executed by applying the preparation example 4 having excellent efficacy effects to the formulation.

Prescription example 1-lotion

Table 5 shows a prescription example of a cosmetic lotion among cosmetic products containing a fermented extract of oleander mushroom .

ingredient content
(Unit: weight%) Formulation Example 1 Formulation Example 2 Preparation Example 4
glycerin
1.3-butylene glycol
Page 1500
Allantoin
DL-panthenol
EDTA-2Na
Benzophenone-9
Sodium hyaluronate
ethanol
Octic dodeceth-16
Polysorbate 20
Preservative, fragrance, coloring
Distilled water -
glycerin
1.3-butylene glycol
Page 1500
Allantoin
DL-panthenol
EDTA-2Na
Benzophenone-9
Sodium hyaluronate
ethanol
Octic dodeceth-16
Polysorbate 20
Preservative, fragrance, coloring
Distilled water 3.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
Balance Sum 100

Prescription example 2-cream

Table 6 shows an example of the prescription of the cream in the cosmetic composition containing the fermented extract of oxal mushroom .

ingredient content
(Unit: weight%) Formulation Example 3 Formulation Example 4 Preparation Example 4
Lipophilic glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hydrogenated vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaluronate
Preservative, fragrance, coloring
Distilled water -
Lipophilic glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hydrogenated vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaluronate
Preservative, fragrance, coloring
Distilled water 3.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100

Prescription example 3-Essence

Prescription examples of the essence in the cosmetic composition containing the fermented extract of oleander mushroom are shown in Table 7 below.

ingredient content
(Unit: weight%) Formulation Example 5 Formulation Example 6 Preparation Example 4
glycerin
Betaine
PAGE 1500
Allantoin
DL-panthenol
E.D.T.A-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaluronate
Carboxyvinyl polymer
Triethanolamine
Octyldodecanol
Octyldodeseth -16
ethanol
Preservative, fragrance, coloring
Distilled water -
glycerin
Betaine
PAGE 1500
Allantoin
DL-panthenol
E.D.T.A-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaluronate
Carboxyvinyl polymer
Triethanolamine
Octyldodecanol
Octyldodeseth -16
ethanol
Preservative, fragrance, coloring
Distilled water 3.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
Balance Sum 100

Example 1 -Evaluation of wrinkle improvement effect of cosmetic products containing fermented extract of Oyster mushroom

The effect of improving the wrinkles of the cosmetic product containing the extract of the pine cone mushroom of the present invention was evaluated through an actual use test. It was evaluated using the cream of Formulation Example 3 containing 3% (v/v) of the extract of Oyster Mushroom and the cream of Formulation Example 4 in which the extract was replaced with purified water. Thirty 30-40-year-old women were randomly divided into two groups, and the cream of the formulation example was washed twice each morning and evening, and then the appropriate amount of cream was applied continuously for 2 months centering around the eyes. The wrinkle improvement effect of each subject was evaluated through visual observation. The experimental results are shown in Table 8 below.

division Excellent wrinkle improvement effect Wrinkle improvement effect No wrinkle improvement effect Formulation Example 3 8 6 6 Formulation Example 4
(Comparative example) 3 4 13

As a result, the cosmetic composition containing the extract of the oleander mushroom of the present invention showed a higher wrinkle improvement effect compared to the comparative example, and the skin irritation could not be observed in the subjects who applied the cosmetic material of the present invention to the skin. .

Example 2 -Evaluation of wrinkle improvement effect of cosmetics containing fermented extract of Oyster mushroom

The skin elasticity effect of the cosmetic of the present invention was evaluated through actual use tests. It was evaluated using the cream of Formulation Example 3 containing 3% (v/v) each of the extract of Osoleus Mushroom and the Cream of Formulation Example 4 in which the extract of Osoleus Mushroom was replaced with purified water. Thirty 30-40-year-old women were randomly divided into two groups, and the creams of Formulation Example 3 and Formulation Example 4 were washed twice each morning and evening, and the appropriate amount of cream was applied continuously for 2 months around the cheeks. The effect of improving the skin elasticity of each subject was evaluated by using a cutometer (C+K, Germany) to improve the elasticity. The experimental results are shown in Table 9 below.

division Excellent wrinkle improvement effect Wrinkle improvement effect No wrinkle improvement effect Formulation Example 3 7 10 3 Formulation Example 4
(Comparative example) 3 5 12

Looking at Table 9, the cream of the formulation example 3 containing the extract of the mushrooms of the present invention showed a higher skin elasticity promoting effect compared to the cream of the formulation example 4 without the extract of the pine mushrooms, and the cosmetic of the present invention was applied to the skin. Skin irritation could not be observed in the subjects in the applied area.

Claims (6)

Trichaptum abietinum ) A cosmetic composition for improving skin wrinkles containing fermented extract as an active ingredient. Trichaptum abietinum ) Antioxidant cosmetic composition comprising fermented extract as an active ingredient. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the fermented extract of oleander mushroom is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. 3. The cosmetic composition for antioxidant according to claim 2, wherein the fermented extract of oleander mushroom is contained in an amount of 0.0001 to 30.0% by weight relative to the total weight of the cosmetic composition. The method of claim 1, wherein the fermented extract of oleander mushroom is Lactobacillus acidophilus , Bifidobacterium lactis , or Lactobacillus acidophilus . And Bifidobacterium lactis ( Bifidobacterium lactis ) cosmetic composition for improving skin wrinkles, characterized in that fermented with a mixed strain mixed in the same weight ratio. The method according to claim 2, wherein the fermented extract of oleander mushroom is Lactobacillus acidophilus , Bifidobacterium lactis , or Lactobacillus acidophilus ( Lactobacillus acidophilus ). And Bifidobacterium lactis ( Bifidobacterium lactis ) is a cosmetic composition for antioxidant, characterized in that fermented with a mixed strain mixed in the same weight ratio.