KR101917711B1 - Cosmetic composition for improving skin wrinkle comprising fermented extract of sonchus oleraceus - Google Patents

Abstract

The invention sow-thistle (Sonchus oleraceus L) as a fermentation extract of a cosmetic composition for improving skin wrinkles containing as an active ingredient, a sow-thistle fermentation extract as an active ingredient based on the total weight of the cosmetic composition 0. 001 ~ 30.0 By weight. According to the invention, the sow-thistle (Sonchus oleraceus L ) fermented extract can provide a cosmetic composition having an excellent effect on anti-aging and skin wrinkle improvement.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for improving skin wrinkles,

The present invention is directed to a method oleraceus L ) fermented extract as an active ingredient.

A variety of physical and chemical changes occur in human skin during the aging process. It is divided into intrinsic aging and photo-aging depending on the cause, and researches on it have been actively conducted. Free radicals can be activated by factors such as ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. When such conditions are intensified, the antioxidant defense network existing in the living body is destroyed, Thereby promoting adult diseases and aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

Protein oxidation is a process in which collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are the connective tissues of the skin, are severed, When it gets worse, mutation of DNA leads to mutation, cancer, and immune function. Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by vigorous metabolism The skin can quickly recover and maintain healthy skin.

As characterized by aging skin, elastin fibers in the skin form crosslinks inside the dermis with collagen fibers, and are involved in skin elasticity. With age, the skin is histologically characterized by a deficiency of elastin fibers and aging, a decrease in collagen fibers, an increase in inflammatory cell infiltration, and a dramatic increase in the activity of the elastinase, elastase. Therefore, the elasticity of the skin is rapidly lowered by the action of elastase. Studies on natural products having elastase inhibitory activity have been continued so far as a solution that can reduce skin aging in a short period of time by inhibiting elastase.

In addition to free radicals, senescence also involves an enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation. Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity.

Oxygen is an indispensable substance in our life with water, but once it enters our body, it turns into highly active oxygen by various causes to oxidize blood vessel cells. Today, about 90% of the causes of the disease have been identified by this active oxygen. The human body is composed of about 60 trillion cells, and the cell membrane is formed as an impetium, so active oxygen is easily connected. When the oxidation reaction occurs, the unsaturated fatty acid changes to lipid peroxide. This lipid peroxidase damages gene DNA, RNA, protein, cell membrane, and cell structure, leading to cancer, causing many adult diseases, and ultimately causing aging. It is called antioxidant substances that act to prevent cells from oxidizing by neutralizing or eliminating these free radicals. Generally, when peroxidation is caused by lipid active oxygen, which is a constituent of skin cells, the cell membrane structure is modified, resulting in cell permeability, fluidity, inactivation of membrane enzymes and transport proteins, cleavage of protein chains, DNA and RNA damage Skin cells are known to cause aging. For this reason, efforts to remove active oxygen have continued, and as a result, many antioxidants have been developed. Materials known to have antioxidative action so far include ascorbic acid, beta-carotene, dibutylhydroxyltoluene (BHT), and DL-alpha-tocopherol . However, since these materials are used as synthetic materials, they cause problems of skin safety when they are used for a long period of time or when they are used in large amounts, or they are limited in their use due to problems in stability when they are contained in cosmetics. to be. Many researchers are working hard to solve these problems.

Korean Patent No. 10-1405429 discloses a whitening effect of cosmetics containing one or more wild grasses such as flower buds, cobwebs, and dung buds.

The inventors have a result of searching various bioactive skin for the natural plant material to improve the aging, sow-thistle in natural plant extracts (Sonchus oleraceus L ) fermented extract showed better antioxidant activity, elastase inhibitory activity, collagen synthesis promotion effect, and MMP-1 production inhibitory effect than the cocoon extract, confirming that the wrinkle of the skin can be effectively improved, .

The present invention is directed to a method oleraceus L ) fermented extract of the present invention.

Preferably, the fermentation extract of Prunus persica is a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, and mixtures thereof. The fermented extract is fermented for 5 to 10 days at a temperature of 28 to 42 캜, Respectively.

Preferably, the dill fermented extract is fermented with a Bacillus strain.

Preferably, the dill fermented extract of the present invention contains 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, Lt; / RTI >

The present invention is directed to a method oleraceus L ) fermented extract as an active ingredient. The active ingredients contained in the cosmetic composition of the present invention is a sow-thistle (Sonchus oleraceus L ) fermented extract has excellent collagen biosynthesis and excellent inhibitory effect on MMP-1 production, thereby improving wrinkles and preventing aging.

The present invention is directed to a method oleraceus L ) fermented extract as an active ingredient.

The shit used in the present invention ( Sonchus Oleraceus L ) is a biennial plant of the Asteraceae family of the dicotyledonous plants, and is commonly found in Korea, China and Japan. Its overall appearance is similar to thistle, but unlike thistle, it has no thorns and is soft. Cutting stems or leaves reveals white juice from cuts. This white juice turns into a brownish sticky liquid over time, and it is said that this looks like a shit. It is used as a herbal medicine for all kinds of herbal remedies such as bamboo shoots, bamboo shoots, green tea leaves, and chestnuts, and is collected and dried between summer and fall. It is mainly effective for fever, detoxification, etc. It is also known to be effective for dysentery, anemia and indigestion.

In the present invention, the bivalve myrtle fermented extract is obtained by extracting from various organs or parts (e.g., leaves, bark, branches, flowers, roots, fruits, stems, etc.) , And most preferably, obtained from the outpost including the bloomed flower.

In the present invention, the fermented extract of Shoko mushrooms means fermented mushroom extract. The cowpox extract may be prepared using conventional solvents under conventional conditions of temperature and pressure, but is preferably (a) water, an anhydrous or lower alcohol having 1 to 4 carbon atoms (e.g., methanol, A solvent selected from the group consisting of ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, (B) decompression by carbon dioxide, supercritical extraction by high temperature, or (c) ultrasonic extraction.

In addition, a fermented milk shrimp fermented extract obtained by fermenting a microorganism inoculated with a culture medium prepared by adding the above-described shit extract to a conventional microorganism culture medium is used.

Specifically, the fermented milk extract of the present invention can be prepared as follows. The microorganism selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, and a mixture thereof is added to the culture medium in an amount of 10,000 to 100,000 CFU / L in an amount of 1 to 30% by weight based on the total culture medium weight, Lt; / RTI > The incubation temperature may be in the range of 28 to 42 DEG C, and preferably 30 to 37 DEG C. The PH may be from 5 to 8 and aerobically or usually anaerobically cultured for about 5 to 10 days and then obtained by aging and filtration.

In the culture medium to be used for fermentation, the amount of the dung extract may be 1 to 30% by weight based on the total culture medium weight. If the amount of the shit extract is less than 1% by weight, the amount of the fermentation broth due to the microorganism is small, and the effect is difficult to be exhibited. If the amount exceeds 30% by weight, the shit extract is insoluble in the medium,

The fermentation strains used in the fermentation process may be selected from yeasts, lactic acid bacteria, Bacillus species, and mixtures thereof. Preferably, strains of Aspergillus sp., Bacillus sp., Lactobacillus sp. .) Can be used.

Examples of the Lactobacillus strains include Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus (Lactobacillus heterohiochii, Lactobacillus herbarium, Lactobacillus hilgardii, Lactobacillus homohiochii, Lactobacillus jensenii, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus casei (subspecies) Lactobacillus casei subsp. Pseudoplantarum, Lactobacillus casei, Lactobacillus casei, Lactobacillus caselactis, Lactobacillus leichmannii, Lactobacillus caseisubsp. Casei, Lactobacillus casei subsp. Pseudoplantarum, Lactobacillus casei subsp. Rh amnosus, Lactobacillus casei subsp. tolerans, Lactobacillus catenaformis, Lactobacillus cellobiosus, Lactobacillus collinoides, Lactobacillus comnifus, Lactobacillus spp. Lactobacillus sp. Coryniformis, Lactobacillus sp. Coryniformis, Lactobacillus sp. Coryniformis, Lactobacillus sp. Coryniformis, Lactobacillus sp. Coryniformis, (Lactobacillus coryniformis subsp. Lactobacillus licheniformis, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus delbrueckii (Lactobacillus spp.), Lactobacillus spp., Lactobacillus spp. Lactobacillus fructus, Lactobacillus fermentum, Lactobacillus fructivorans, Lactobacillus fructosus and Lactobacillus helveticus may be used, and more preferably Lactobacillus fructivorans, Lactobacillus spp. Lactobacillus bulgaricus is preferred.

Examples of the Aspergillus strain include Aspergillus oryzae, Aspergillus niger, Aspergillus, and more preferably, Aspergillus oryzae, Aspergillus oryzae is preferred.

Examples of the above Bacillus strains include Bacillus acidocaldarius, Bacillus alcalophilus, Bacillus alvei, Bacillus anthracis, Bacillus badius, Such as Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus fastidiosus, Bacillus lentimorbus, Bacillus spp. Bacillus licheniformis, Bacillus macerans, Bacillus macquariensis, Bacillus megaterium, Bacillus mycoides, Bacillus licheniformis, Bacillus licheniformis, Bacillus macquariensis, Bacillus pantothenticus, Bacillus pallidus pasteurii), Bacillus sp. Bacillus popiliae, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, Bacillus subtilis, Bacillus subtilis, , And Bacillus thuringiensis. Bacillus subtilis is preferably used. Bacillus subtilis is preferably Bacillus subtilis.

The fermented extract of Sonchus oleraceus L is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 10.0% by weight, based on the total weight of the cosmetic composition. When the content of the fermented extract is less than 0.001% by weight, the skin improvement effect is not exhibited. When the content is more than 30.0% by weight, the degree of increase in the skin improving effect against the increase of the content is insignificant, It is not economical.

Therefore, a variety of such poisonous feces ( Sonchus oleraceus L) fermented extract of the present invention has an excellent antioxidative effect, an elastase inhibitory activity, a collagen biosynthesis effect, and an inhibitory effect on MMP-1 production.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, , And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan, and the like.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

On the other hand, the cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention. The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention.

The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for describing the present invention more specifically, and the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention

Manufacturing example  1 - Room shit ( Sonchus oleraceus  L) Fermentation Extract Preparation

The shit outposts were washed with clean water, and then cut to a predetermined size. The mixture was refluxed three times for 5 hours with 70% (v / v) ethanol aqueous solution, cooled, and filtered through Whatman # 4 filter paper. The filtrate extract (2L 70% EtOH) was concentrated under reduced pressure at 50 DEG C or lower to adjust the water content to 70% by adding water to obtain a fermentation raw material so that the fermentation microorganism can be cultured well. Subsequently, the obtained fermentation raw material was sterilized at 121 DEG C for 15 minutes. Bacillus subtilis (3%) was inoculated on the medium and incubated at 37 ° C for 10 days under aerobic conditions at 37 ° C. Then, the culture was filtered using a filter paper of 0.25 μM, and the filtrate was aged at 4 ° C. for 10 days at a low temperature, followed by final filtration using a 0.25 μM filter paper. The thus prepared fermentation broth is referred to as "bifid fermentation extract ", which is intended to explain the invention in more detail. The scope of the fermentation broth used in the preparation of bifid fermentation extract is not limited thereto .

Comparative Manufacturing Example  1 - Room shit ( Sonchus oleraceus  L) Solvent Extract Preparation

The shit outposts were washed with clean water, and then cut to a predetermined size. The mixture was refluxed three times for 5 hours with 70% (v / v) ethanol aqueous solution, cooled, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower. The dried extract was diluted to a certain concentration and used in the following experiment.

Experimental Example  One - DPPH  Radical Scatters  Measure

In order to measure the antioxidative effect of the above Production Example 1, L-ascorbic acid was used as a comparative sample under laboratory conditions and the antioxidative activity was measured using the DPPH method.

The DPPH method is to measure the antioxidative activity by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of reduction of the absorbance by reduction of DPPH by the test substance is compared with the absorbance of the blank test solution and the free radical scavenging ratio is measured at a wavelength of 560 nm. As the reagent used, 0.2 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol and adjusted to 100 ml. As a measurement method, 0.15 ml of a 0.2 mM DPPH solution and 0.15 ml of a sample solution are added to a 96-well plate, stirred rapidly, and incubated at 25 DEG C for 10 minutes. Thereafter, the absorbance St at 560 nm is measured. The blank test is carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt is measured. The blank of the sample solution is measured by the same procedure using methanol instead of 0.2 mM DPPH solution to determine the absorbance Bo.

The results are shown in Equation (1), and the results are shown in Table 1. L-ascorbic acid was used as a comparative group

 St: absorbance at 560 nm after free radical scavenging of the sample solution

 Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

 So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

 Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample density Scatters  ( %  ) Comparative Preparation Example 1 1000 65.12 ± 2.31 Production Example 1 83.97 ± 3.09 Comparative Preparation Example 1 500 42.51 + - 3.73 Production Example 1 54.87 ± 1.67 Comparative Preparation Example 1 100 17.67 ± 2.97 Production Example 1 34.41. + -. 3.41

As can be seen from Table 1, the free radical scavenging ratio of the rot fungus extract (Preparation Example 1) according to the present invention is superior to that of the common solvent extract of the fungus (Comparative Preparation Example 1).

Experimental Example  2 - Elastase ( Elastase ) Inhibition activity measurement

Elastase is an enzyme that degrades elastin, an important substrate for maintaining skin elasticity in the dermis. Elastase is also a nonspecific hydrolytic enzyme capable of degrading collagen, another important substrate protein. Thus, the elastase inhibitor has a function of improving the wrinkles of the skin, and the ursolic acid and the like are used as the elastase inhibitor. Elastase is known as an enzyme that breaks elastin, the insoluble elastic fiber protein of animal connective tissues, and breaks the network structure of the dermal tissue of the skin to produce wrinkles. In the dermis of the skin, elastin related to the elasticity of collagen and skin forms a network structure. As the network structure is broken, the elastin is decomposed by elastase, and the skin is wrinkled and wrinkles are generated. do. Therefore, skin aging can be suppressed by lowering the activity of elastase, elastase, which is one of the main causes of skin aging.

The amount of p-nitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 445 nm using N-succinyl- (L-Ala) 3-p-nitroanilide, S4760, Sigma as a substrate. 500 μL of each test solution was added to 500 μL of each test solution. 500 μL of porcine pancreas elastase (E1250, Sigma) (2.5 U / mL) dissolved in 2 mM tris-HCl buffer (pH 8.6) N-succinyl- (L-Ala) 3-p-nitroanilide (500 μg / mL) dissolved in tris-HCl buffer (pH 8.6) was added and reacted for 20 minutes. Elastase inhibitory activity was calculated by the following equation (2) as the absorbance reduction rate of the sample solution addition group and the no addition group.

Name of sample Treatment concentration (ppm) Elastase inhibitory activity (%) Comparative Preparation Example 1 100 5.129 Production Example 1 20.229 Comparative Preparation Example 1 500 17.815 Production Example 1 46.713 Comparative Preparation Example 1 1000 33.782 Production Example 1 68.192

As can be seen from Table 2, it was found that the inhibitory activity against the elastase of the spinach extract (Preparation Example 1) according to the present invention is superior to that of the solvent extract of the rotifers (Comparative Preparation Example 1).

Experimental Example  3 - ELISA Kit  Results of collagen biosynthesis assay

Human fibroblasts (Human Skin Fibroblast) for 37 ℃, 5% in a CO ₂ incubator of 10% in the cell culture medium to maintain a constant humidity FBS, Penicillin (50U / ml) , Streptomycin DMEM (Dulbecco's containing (50 / ml) (Gibco, USA). Cells were seeded at a density of 1 × 10 ⁵ cells / ml in a 24-well plate and cultured for 24 hours. In the fibroblasts, collagen type I was quantified by immunosorbent assay (ELISA) using Procollagen Type I c-peptide EIA kit (TaKaRa, japan) for 48 hours The cultured supernatant of the cell culture was carefully collected. First, 100 μl of the culture supernatant, which was cultured on a strip coated with antibody against Procollagen Type I c-peptide for 48 hours, was added and reacted at 37 ° C for 2 hours. After the reaction, unreacted material was removed by washing three times using a washing buffer, and 100 μl of blocking buffer was added thereto, followed by reaction at 37 ° C for 2 hours. After reaction, unreacted material was removed by washing three times using a washing buffer, and a solution in which POD was conjugated to another antibody binding to 'Procollagen Type I c-peptide' (Antiobody- POD conjugate solution) was added and reacted at 37 ° C for 2 hours. After the reaction, the unreacted material was removed by washing three times using a washing buffer, the same amount of substrate solution was added, and the reaction was carried out for 30 minutes. Then, stop solution (1N H2SO4) Terminated. The absorbance of the strips after the experiment was measured at 450 nm, and the amount of newly synthesized collagen was measured by the amount of PICP in comparison with the collagen standard. The effect of collagen synthesis is shown in Table 3.

Name of sample density Collagen synthesis amount (ng / 2x10 ⁴ ) Comparative Preparation Example 1 200 146 ± 5.74 Production Example 1 214 ± 7.12 Comparative Preparation Example 1 100 87 ± 4.07 Production Example 1 134 ± 8.91

As can be seen from the above Table 3, the amount of collagen produced in Production Example 1 according to the present invention is superior to Comparative Production Example 1, and the effect of improving wrinkles is excellent.

Experimental Example  4 - MMP -1 production inhibitory effect

In Experimental Example 4, it was found that the extract obtained from Preparation Example 1 had the effect of inhibiting the production of MMP-1 TGF-beta (10 ng / ml, Roche, USA) was used as a comparative group to inhibit the production of MMP-1. Human normal skin cells, fibroblasts (Korean Cell Line Bank, Korea), were inoculated into 48-well microplates (Nunc, Denmark) at a density of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, Serum-free DMEM medium supplemented with the final concentration of 100 mu g / ml of the shark fermentation extract of Preparation Example 1 for 24 hours, and serum-free DMEM medium not containing the shark fermentation extract for 48 hours And further cultured. After the incubation, the supernatant of each well was pooled and the amount (ng / ml) of the newly synthesized MM P-1 was measured using an MMP-1 assay kit (Amersham, USA) The percent inhibition (%) was calculated and the results are shown in Table 4.

Name of sample Treatment concentration Inhibition rate of MMP-1 production (%) Comparative Preparation Example 1 200 44.17 + - 3.47 Production Example 1 56.92 + - 4.11 Comparative Preparation Example 1 100 24.37 ± 2.99 Production Example 1 41.26 + 5.08

As shown in Table 4, it can be seen that the MMP-1 production inhibitory rate of the mushroom fermented extract according to the present invention (Preparation Example 1) is superior to that of the solvent extract (Comparative Preparation Example 1).

Prescription example  One -  cream

Table 5 shows the prescription examples of the cream in the cosmetics containing the extract of fermented soybean curd refuse.

Formulation Example 3 Content (unit:% by weight) (Example 1)
Pro-type glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water 3.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100

Prescription example  2 -  toilet water

Shit ( Sonchus oleraceus L) fermented extract is shown in Table 6 below.

Formulation Example 1 Content (unit:% by weight) (Example 1)
glycerin
1,3-butylene glycol
PIZZY 1500
Allantoin
DL-Panthenol
EDTA-2Na
Benzophenone-9
Sodium hyaruronate
ethanol
Octidodeces-16
Polysorbate 20
Preservative, fragrance, pigment
Distilled water 3.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
Balance Sum 100

Prescription example  3 - Essence

Table 7 shows the prescription examples of the essence in cosmetics containing the extract of bark (Sonchus oleraceus L).

Formulation Example 5 Content (unit:% by weight) (Example 1)
glycerin
Betaine
PIZZY 1500
Allantoin
DL-Panthenol
Lee D. T.-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaruronate
Carboxyvinyl polymer
Triethanolamine
Octyldodecanol
Octyldodec-16
ethanol
Preservative, fragrance, pigment
Distilled water 3.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
Balance Sum 100

Comparative Prescription Example  1 - Cream

Table 8 shows the prescription examples of the cream which does not contain the fermented extract of dung fungus.

Comparative Prescription Example 1 Content (unit:% by weight) Pro-type glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water 2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100

Experimental Example  5-sprout containing fermented extract of shit Cosmetic  Wrinkle improvement effect evaluation

The effect of improving the wrinkles of the cosmetic compositions containing the extract of the mackerel fermented according to the present invention was evaluated through practical use tests. The bark extract was evaluated using the cream of Formulation Example 1 containing 3% (v / v) and the cream of Comparative Formulation Example 1 in which the extract was replaced with purified water. Thirty women aged 30-40 years were randomly divided into two groups. Each cream was washed every morning and two times in the evening, and then the cream was applied continuously for two months, centering on the eyes. The wrinkle improvement effect of each subject was evaluated by visual observation. The experimental results are shown in Table 9 below.

Excellent wrinkle improvement effect Wrinkle improvement effect slightly No effect of wrinkle improvement Prescription Example 1 13 10 7 Comparative Prescription Example 1 6 9 15

As a result, the cosmetic composition (Formulation Example 1) containing the mushroom fermentation extract of the present invention exhibited a higher wrinkle-reducing effect than the Comparative Formulation Example 1 which did not contain the mushroom extract of the present invention. No skin irritation could be observed.

Claims (5)

Sow-thistle (Sonchus oleraceus L) of Bacillus subtilis (Bacillus subtilis) as fermented sow-thistle extract fermentation cosmetic composition for improving skin wrinkles containing. The method according to claim 1,
The cosmetic composition for improving skin wrinkles is obtained by fermenting for 5 to 10 days while maintaining a pH of 5 to 8 under a temperature condition of 28 to 42 캜. The method according to claim 1,
The cosmetic composition for improving skin wrinkles according to any one of claims 1 to 3, The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the cosmetic composition is a cosmetic composition for improving skin wrinkles. delete