KR20160100745A

KR20160100745A - Functional Cosmetic Compositions of Bio-Conversion using Solid State Fermentation and Functional Cosmetics Produced Thereby

Info

Publication number: KR20160100745A
Application number: KR1020150023639A
Authority: KR
Inventors: 김종명; 신용규; 김재희; 이재우; 이상한; 손형우
Original assignee: (주)팜바이오스
Priority date: 2015-02-16
Filing date: 2015-02-16
Publication date: 2016-08-24

Abstract

Using the solid fermentation method, which uses the fungicide including medicinal mushroom fungus to produce new physiologically active substance from the existing substance by the approach of bioconversion, the biodegradable functional cosmetics with complex functions such as wrinkle improvement, whitening, and antioxidant (Ganoderma lucidum L.) as an active ingredient in a culture solution obtained by aseptic inoculation of mung bean (Phaseolus radiatus L.) and licorice (Glycyrrhiza uralensis Fischer) A combination function of antioxidation, anti-inflammation, whitening, and wrinkle improvement can be realized.

Description

TECHNICAL FIELD [0001] The present invention relates to a bio-conversion functional cosmetic composition using solid fermentation, and functional cosmetics prepared therefrom.

The present invention relates to a bio-conversion functional cosmetic composition using solid state fermentation and a functional cosmetic composition prepared therefrom, and more particularly to a cosmetic composition which is obtained by performing solid fermentation using Ganoderma lucidum and licorice, , A cosmetic composition having a complex function such as wrinkle improvement, whitening, and antioxidation by using solid fermentation which generates a new physiologically active substance from existing substances, and a functional cosmetic product prepared therefrom.

Generally, the skin of a person is excessively secreted from the sebaceous glands, the pores can not be smoothly discharged through the pores, or the pores are blocked by keratin, various wastes, and debris, and the sebaceous glands become inflamed, resulting in skin troubles such as acne .

In particular, if the sebum secretion increases after puberty in earnest, the pores, which are the way out of sebum to spread out, become wider and the keratin and sebum become easier to stagnate in the pores, and the pores become larger and larger and suffer from chronic acne The repeated inflammation leads to a vicious cycle in which pores are inevitably wider.

Even if you do not have a lot of sebaceous glands, if you get older, everyone will have a certain amount of pores, and women will begin to depress skin elasticity around 30 years of age, and the skin will begin to sag and the strength to tighten pores will also fall.

In addition to such skin troubles, various environmental factors, such as industrial pollution, environmental pollution, increased use of food additives, increase in use of carpet, bed, sofa due to western style of residence, and house dust mite Skin diseases such as dry skin, eczema, and atopy are increasing due to the increase of allergen (allergen), genetic sweep, immunological reaction and skin barrier abnormality.

In addition, for the prevention and treatment of inflammation such as acne and skin troubles, most of the drugs currently prescribed by the hospital are steroid drugs or antihistamines, which are not the fundamental drugs but therapeutic agents for the palliation of temporary lesions. Overdose and long-term use, there are abnormalities of the body's immune system, increased risk of infection, side effects of supporting tissues such as bones, muscles and skin, hypertension and osteoporosis, And the growth disorder of girls.

Recently, functional powders, cosmetics, and soaps using various natural materials capable of preventing and treating various skin diseases as described above have been proposed.

In other words, various green growth projects are attracting attention with the theme of natural materials, environment friendly, and harmless to human body, and the demand for natural materials and organic cosmetics is increasing accordingly. In addition, fermentation technology is attracting attention as a way to maximize their efficacy and effectiveness, as well as extracts of natural materials. Among them, the fermentation yeast fermentation method is a technology that promotes penetration of the skin by lowering the nutrients and promotes penetration of the skin, and makes it possible to feel various effects such as moisturizing and wrinkle at one time due to hypoactivity due to high concentration of amino acids similar to skin moisturizing factor , Development of new materials combining the existing herbal extract material with such enrichment and fermentation technology is underway.

Examples of such techniques are described in documents 1 and 2 below.

For example, in Patent Document 1, 0.6 weight part of pearl powder, 1.4 weight part of red ginseng powder, 20 weight part of green tea leaf powder, 20 weight part of mugwort powder, 20 weight part of kelp powder, 20 weight part of mulberry leaf powder 20 parts by weight of licorice powder, 20 parts by weight of ganoderma powder, 20 parts by weight of persimmon leaf powder, 20 parts by weight of Angelica powder, 20 parts by weight of black sesame powder, 80 parts by weight of Yulmu powder, 80 parts by weight of red bean powder, Wherein said powder is selected from the group consisting of soybean powder, pearl powder, red ginseng powder, green tea leaf powder, mugwort powder, kelp powder, mulberry leaf powder, licorice powder, ganoderma powder , The persimmon leaf powder, the Angelica keiskei powder, the black sesame powder, the yulmu powder, the red bean powder, the brown rice powder, and the mung bean powder are selected from soybean, pearl, red ginseng, green tea leaf, wormwood, kelp, mulberry leaf, licorice, Persimmon leaves, angelica, black sesame, yulmu, red beans, brown rice and It is disclosed for a massage powders for pulverized to each dried two.

Also, Patent Document 2 discloses a method in which 1 to 20 wt% of stearic acid, 1-15 wt% of palmitic acid, 1-20 wt% of lauric acid, 1 to 30 wt% of triethanolamine, 1 to 15 wt% of glycerin, 1 to 10% by weight of corn starch, 1 to 10% by weight of corn starch trefolia fruit liquid, 1 to 10% by weight of ginseng, Angelica gigas, Irises, Lycopersicum, Gosam, Junjou, Licorice, Astragalus, Dermis, 1 to 5% by weight of ginseng, 1 to 10% by weight of Angelica gigantis, 1 to 10% by weight of Angelica keiskei koidz., Iris 1 1 ~ 10 weight%, ginseng 1 ~ 5 weight%, ginseng 1 ~ 10 weight%, licorice 1 ~ 10 weight%, licorice 1 ~ 8 weight%, dermis 2 ~ 10 weight% A skin care composition comprising a medicinal herb comprising 1 to 10% by weight of a pine needle, 1 to 6% by weight of a white paper, 1 to 5% by weight of natural grass, 1 to 5% by weight of mung bean, 1 to 5% Disclosed is a cosmetic soap for atopic skin containing There is.

Korean Registered Patent No. 10-0861688 (registered on September 29, 2008) Korean Registered Patent No. 10-1037017 (registered on May 18, 2011)

However, in the conventional techniques as described above, there is a problem that the extraction method of medicinal plants is troublesome and the components of medicinal plants can not be extracted sufficiently by simply extracting using medicinal plants or extracting them with liquid fermentation.

In addition, there is also a problem that it is difficult to produce a composition that realizes a complex function of antioxidation, anti-inflammation, whitening, and wrinkle improvement.

DISCLOSURE OF THE INVENTION The object of the present invention is to solve the problems as described above, and it is an object of the present invention to provide a solid fermentation improved in functions such as whitening and wrinkle improvement as well as decolorization, deodorization, antioxidant and anti- And a functional cosmetic product prepared from the same.

It is another object of the present invention to provide a biodegradable cosmetic composition using solid fermentation capable of sufficiently extracting components of a medicinal plant through solid fermentation and functional cosmetics prepared therefrom.

To achieve the above object, the functional cosmetic composition according to the present invention is characterized in that an extract obtained by culturing Ganoderma lucidum L. cultured by inoculating aseptically in Ganoderma lucidum L. and Glycyrrhiza uralensis Fischer is effective As a component.

In the biofunctional cosmetic composition according to the present invention, the fermentation is characterized by solid state fermentation.

In the biodegradable functional cosmetic composition according to the present invention, the ginger mushroom microorganism is cultured in a medium at 20 to 35 ° C. for 10 to 30 days, subcultured at intervals of 4 weeks and stored at 4 ° C., The green mung bean and licorice are washed and removed impurities and sterilized by high pressure at 121 ° C for 20 minutes under moisture control, and then cooled at room temperature.

In the biofunctional cosmetic composition according to the present invention, the solid fermentation is carried out for 10 to 30 days in an incubator at a temperature of 20 to 35 DEG C, a pH of 4 to 7.5 and a relative humidity of 40 to 80%, and the extract is subjected to the solid fermentation After 5 to 20 times as much volume of distilled water and 10 to 60% of Et-OH as a solvent, the mixture was shaken for 1 to 5 hours and then immersed for 24 to 96 hours. After centrifugation at 10,000 rpm for 10 minutes, supernatant was obtained and extracted .

In the biofunctional cosmetic composition according to the present invention, the composition may be a paste, a gel, a cream, a lotion, a powder, a solid soap, a wipe, a shampoo, a rinse, a solution, a suspension, an emulsion, a mineral cosmetic, - containing cleansing or non-surfactant cleansing, oil, powder foundation, emulsion foundation, hair dye, wax, supple lotion, nutritional lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, pack, , Perfume, ointment, cleansing water, powder, wax foundation, and spray.

As described above, according to the present invention, the bio-conversion functional cosmetic composition using the solid fermentation and the functional cosmetic composition prepared therefrom are biologically transformed by the solid fermentation through mung bean and licorice, so that the antioxidative, anti- , A wrinkle improvement function can be realized.

Further, according to the cosmetic composition for functional conversion using solid fermentation according to the present invention and the functional cosmetic composition prepared therefrom, since the solid fermentation is carried out through Ganoderma lucidum, Mung bean and licorice, the fermentation process can be simplified and easily realized .

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing the biotransformation state of fungus fermented ginseng applied to the present invention,
FIG. 2 is a process diagram for explaining a process of manufacturing a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
FIG. 3 is a photograph showing the state before the fermentation of licorice, green bean and manure according to the present invention,
FIG. 4 is a photograph showing progress of solid fermentation of licorice, green bean and manure according to the present invention,
FIG. 5 is a photograph showing the state after the fermentation of licorice, green bean and manure according to the present invention,
FIG. 6 is a photograph of the solid fermentation extract according to the present invention prepared before and after the column,
FIG. 7 is a graph showing the results of measurement of DPPH radical scavenging activity in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
8 is a graph showing the results of FRAP measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
9 is a graph showing the results of CUPRAC measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
FIG. 10 is a graph showing the inhibition of NO activity and the anti-inflammatory effect in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
11 is a graph showing the results of measurement of the total flavonoid content in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
12 is a graph showing the results of measurement of the total polyphenol content in the biofunctional cosmetic composition using solid fermentation according to the present invention,
FIG. 13 is a graph showing the results of measurement of inhibition of elastase in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention,
14 is a graph showing the results of tyrosinase inhibition measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

These and other objects and novel features of the present invention will become more apparent from the description of the present specification and the accompanying drawings.

The bio-functional cosmetic composition using solid fermentation according to the present invention is characterized in that an extract obtained by cultivating Ganoderma lucidum L. cultured by inoculation with mung bean (Phaseolus radiatus L.) and licorice (Glycyrrhiza uralensis Fischer) As a component.

The fermentation applies solid state fermentation.

Solid fermentation using natural materials applied to the present invention induces efficient bioconversion of fermented material through fungal solid fermentation, which is a new method of fermentation completely different from fermentation of bacteria or lactic acid bacteria in liquid state, And the useful bioactive substance is exploited by utilizing the breakthrough fermentation ability of the present invention. One of the results obtained in the present invention is a natural product containing a polyphenolic compound having excellent antioxidant and antiinflammatory activity and a medicinal herb which has been used for a long time in traditional oriental herbal medicine, (Bio-conversion) technology that utilizes solid fermentation to maximize physiological activity.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing the biotransformation state of fungus fermented ginseng applied to the present invention. FIG.

In the present invention, the strain is isolated from fruiting bodies such as Ganoderma lucidum, which has been recognized as a representative medicinal mushroom, and cultured in a PDA medium, followed by pure water separation. Optimal conditions for seed culture activity, temperature, moisture content, pH, oxygen content, etc. were established after seed culture in PD liquid medium so that it could be used as a seed culture. Optimum condition for optimal fermentation temperature, humidity, Respectively.

The ginseng mushroom applied to the present invention has a diameter of 10-20 cm and a length of 5-10 cm and is kidney-shaped. The surface of the fresh mushroom is dark brown to dark brown with dark brown and has a ring groove and radial fine wrinkles. The forehead is covered with the forehead and the varnish is produced by the secretion of varnish. The back side is a hole (microscopic hole), and the bag is a resurfacing or upright type and is blackish brown or black. The tissue is corkoid, separated into two upper and lower layers, the upper layer is a light yellowish brown in the white lower layer. Such gonorrhea mushrooms are produced in live trees and dead wood stumps from early summer through autumn.

Licorice is a medicinal plant. Its roots are reddish brown, deeply penetrates into the ground, and the stem grows to a height of about 1m. The white hairs are dense and appear grayish white, the glandular branches are scattered, the leaves are alternate, and odd numbered compound leaves. These licorice smells unusual and tastes sweet.

Licorice is known to harmonize the toxicity of all medicines so that the medicinal effects are well visible, it regulates the heat and scent of the book, makes good communication of all blood vessels, strengthens muscles and bones, and its pharmacological action is detoxification, hepatitis, urticaria, And so on. It is known to inhibit peptic ulcer, and it has Jinhae, genomic, muscle relaxation, diuretic action, anti-inflammatory action.

Also, mung bean is also called asan bean and 吉豆. It grows well in warm clay loam (dark soil with a moderate mixture of sand and clay) and is 30-80㎝ high. The stem is thin and vertical There is a Mac and there are about 10 nodes and branches. These mung beans are rich in essential amino acids and unsaturated fatty acids, help digestion, and are excellent in urination. They are known to help detoxify rapidly by excretion through the urine, and "mushroom" It is said that it is good for seeing the joy, harmonizing the five gods, stabilizing the mind, regulating the wind, and making the skin beautiful.

Hereinafter, the structure of the present invention will be described with reference to Figs. 2 to 6. Fig.

FIG. 3 is a photograph showing the state before the solid fermentation of licorice, green bean and manure according to the present invention, and FIG. 4 is a photograph showing the progress of solid fermentation of Licorice, Mung Bean and Ganoderma according to the present invention, FIG. 5 is a photograph showing the state after fermentation of Licorice, Mung Bean and Ganoderma according to the present invention, FIG. Of the solid fermentation extract according to the present invention.

As shown in FIG. 2, in the production of a bio-conversion functional cosmetic composition using solid fermentation according to the present invention, a strain and a culture medium are performed (S10).

In step S10, Ganoderma lucidum L. is cultivated in a PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 20 to 35 ° C for 10 to 30 days And were stored at 4 ° C while being subcultured at intervals of 4 weeks. The seeds to be inoculated to the medicinal herb were 'Shin et al. 2010 'were prepared and modified. (1 cm x 1 cm, 3 pieces) were inoculated in 100 ml of 500 ml flask, and then cultured in a rotary shaker incubator (Daihan Labtech Co. Korea, Ganoderma lil (25 ± 1 ° C), 130 rpm) for 7 days Cultured and used as liquid seeds.

Mycelial growth of Phellinus baumii, Ganoderma lucidum and Paecilomyces japonica was determined using seven different media as shown in Table 1 to verify mycelial growth optimum media. The mycelium subcultured in petri dishes was inoculated into at least five petri dishes with a diameter of 5 mm and then fermented in a fermenter at 20 to 35 ° C, pH 4 to 7.5, relative humidity 40 to 80% for 10 to 30 days Mycelial growth was measured after incubation. Table 1 shows the composition of the medium.

Next, mung bean and licorice were purchased from the market of Chilsung, Daegu and the alley of pharmacopoeia for the fermentation of natural products using Ganoderma lucidum.

According to the present invention, the natural fermented product of Ganoderma lucidum according to the present invention was prepared by washing the mung bean and licorice as a fermentation raw material, removing the impurities, sterilizing the product at 121 ° C for 20 minutes, and then cooling the product at room temperature. Gingko mushroom liquid seeds were aseptically inoculated into sterilized green beans and licorice, as shown in Fig. Fermentation was carried out in an incubator at 20 to 35 ° C, pH 4 to 7.5 and relative humidity 40 to 80% for 10 to 30 days as shown in FIG. 4 (S20).

The state after the solid fermentation of mung bean and licorice in step S20 is as shown in FIG.

Next, a fermented extract was prepared (S30).

In step S30, 5 to 20 times volume of distilled water and 410 to 60% of Et-OH solvent are added to the mixture, which is then shaken for 1 to 5 hours, followed by immersion for 24 to 96 hours, centrifugation at 10,000 rpm for 10 minutes The supernatant was obtained, and after repeating this three times, 30% of butylene glycol was added to the extract, followed by filtration, and the filtrate was prepared as follows.

That is, alcohol immersion, fermentation water disruption and extraction, centrifugation, and dilution of the extract were carried out.

In order to remove the color and odor of the fermented solid fermented product, 250-350 mesh Charcoal activated powder (Yakuri pure chemicals Co., Ltd.) and Silicagel (Joey Life) , A 50 cm high column, a 10 cm diameter column and a 100 cm high column were filled at a height of 40 cm. As a result, there was little difference in the color of the silica gel column, The color difference was remarkable in the samples passing through the activated carbon column.

Then, the functional cosmetic composition according to the present invention was completed by sterilizing the biodegradable cosmetic composition using solid fermentation prepared as described above (S40), putting it into a container (S50).

Next, the efficacy of the extract for bioactive functional cosmetic using solid fermentation according to the present invention was examined.

(1) DPPH radical scavenging activity

As a test method for measuring antioxidant activity, a typical DPPH radical scavenging activity assay was performed among various known methods. A solution of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl; Sigma Co.) dissolved in methanol was dispensed into a 96-well plate (190 μl), and each extract was replicated three times After standing at room temperature for 30 minutes, the measurement was carried out at 520 nm using a VICTOR 3 spectrophotometer (1420 Multiabel counter; PerkinElmer, Turku, Finland) and the measured values were determined as DPPH radical scavenging = 1- (ABS _sample / ABS _control ) 100, the activity increased as the extract concentration increased. As shown in FIG. 7, the concentration was significant.

7 is a graph showing the results of measurement of DPPH radical scavenging activity in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(2) Ferric reducing antioxidant power (FRAP) measurement

FRAP measurement is a representative antioxidant measurement method for measuring the ferric reducing ability of a compound. When the Fe-TPTZ (ferric tripyridyltriazine) complex is converted to a blue Fe-TPTZ complex by an antioxidant at pH 3.6, the absorbance is measured to confirm the reduction potential of the compound to be searched. As the reaction solution for the experiment, 10 mM of TPTZ (2,4,6-tripyridyl-s-triazine): 20 mM of FeCl ₃ 6H ₂ O in acetate buffer (pH 3.6, 300 mM) 1 ratio and mix it before use. After the mixture of the reaction mixture (190 μl) and the extract (10 μl), the absorbance was measured at 590 nm for about 10 minutes at intervals of 150 seconds.

8 is a view showing the results of FRAP measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(3) CUPRAC (cupric ion reducing antioxidant capacity) measurement

When the Cu ²⁺ ion is reduced to Cu ⁺ , it becomes yellow when it meets with neocuproine. After mixing 194 μl of DW, ₂ μl of 10 mM CuCl ₂ and ₂ μl of 75 mM Neocuproine, 198 μl of a reaction mixture was added to a 96-well plate containing 2 μl of the sample and reacted in a dry oven at 37 ° C for about 20 minutes The absorbance was measured at 450 nm. As a result of the experiment using ascorbic acid as a control, as shown in FIG. 9, there was a phenomenon in which the activity was increased significantly in the concentration.

9 is a view showing the results of CUPRAC measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(4) NO assay

RAW264.7 cell line was used to inhibit NO activity and anti-inflammatory effects on the solid fermentation extract. RAW264.7 cells were treated at a concentration of 2 × 10 4 cells / well in a 96-well plate and treated with LPS 200 ng / well. After 24 h incubation, inhibition of NO production was measured by concentration of solid fermentation extract. NO concentration was determined by mixing 1: 1 with Greiss reagent (0.1% naphthlyenediamine-dihydrochloride, 1% sulfanilamide, 5% phosphoric acid) and cell culture medium. The absorbance at 520 nm was measured. As shown in FIG. 10, The activity was increased.

FIG. 10 is a graph showing the inhibition of NO activity and the anti-inflammatory effect in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(5) Measurement of total flavonoid content

The content of flavonoid was defined as mg / catechin g by the equation obtained from the standard curve using the absorbance value of catechin. The standard curves showed the highest content of flavonoids in the fermented extracts of mung bean, licorice and manure. The standard curves and calculated values are shown in FIG.

11 is a graph showing the results of measurement of the total flavonoid content in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(6) Measurement of total polyphenol content

The total polyphenol content was determined by using gallic acid as a standard curve. As shown in FIG. 12, the fermented extracts of mung bean, licorice and Ganoderma lucidum showed a large increase in content.

12 is a graph showing the results of measurement of the total polyphenol content in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention.

(7) Elastase Inhibition Measurement

As shown in FIG. 13, the inhibitory activity of the fermented extract was about 50% similar to that of EGCG at a concentration of 300 μg / ml of the extract of licorice, green mung, and Ganoderma lucidum.

FIG. 13 is a graph showing the results of measurement of the elastase inhibition in the biofunctional cosmetic composition using solid fermentation according to the present invention. FIG.

(8) Tyrosinase Inhibition Measurement

As shown in Fig. 14, the tyrosinase inhibitory activity of the fermented extract showed excellent tyrosinase inhibitory activity in the extracts of licorice, mung bean and Ganoderma lucidum.

Fig. 14 is a graph showing the results of tyrosinase inhibition measurement in a bio-conversion functional cosmetic composition using solid fermentation according to the present invention. Fig.

According to the result of measurement as described above, it is possible to produce a biodegradable functional cosmetic product having a complex function of improving wrinkles, whitening, and antioxidation by an approach of bio-conversion which performs solid fermentation by aseptically inoculating Ganoderma lucidum with mung bean and licorice .

That is, the bio-functional cosmetic composition using solid fermentation according to the present invention may be prepared in any formulations conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, , Powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. It may preferably be formulated into a formulation of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

Although the present invention has been described in detail with reference to the above embodiments, it is needless to say that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit of the present invention.

By using the bio-conversion functional cosmetic composition using solid fermentation according to the present invention and the functional cosmetics prepared therefrom, the composite function of antioxidation, anti-inflammation, whitening, and wrinkle reduction can be realized.

Claims

A biotransformation functional cosmetic composition characterized by containing as an active ingredient an extract of Ganoderma lucidum L. obtained by cultivating the mung bean (Phaseolus radiatus L.) and licorice (Glycyrrhiza uralensis Fischer) inoculated with a culture solution.

The method according to claim 1,
Wherein the fermentation is a solid state fermentation.

3. The method of claim 2,
The ginger mushroom was cultured in a medium at 20 to 35 ° C for 10 to 30 days, subcultured at 4-week intervals, stored at 4 ° C,
The mung bean and licorice are washed and removed impurities and sterilized at 121 ° C for 20 minutes under moisture control, and then cooled at room temperature.

3. The method of claim 2,
The solid fermentation is carried out for 10 to 30 days in an incubator at 20 to 35 DEG C, pH 4 to 7.5 and relative humidity 40 to 80%
The extract was prepared by adding 5 to 20 times volume of distilled water and 10 to 60% of Et-OH to the extract, shaking it for 1 to 5 hours, immersing it for 24 to 96 hours, centrifuging at 10,000 rpm for 10 minutes To obtain a supernatant, and then extracting the functional cosmetic composition.

The method according to claim 1,
The composition may be in the form of a paste, gel, cream, lotion, powder, solid soap, water soap, shampoo, rinse, solution, suspension, emulsion, mineral cosmetic, powder perfume, surfactant- Cream, essence, cleansing cream, cleansing foam, pack, pack base, eye cream, perfume, ointment, cleansing water, powder, wax foundation, foundation, emulsion foundation, hair dye, wax, Wherein the cosmetic composition is used in any one of spraying, spraying, and spraying.