KR20130030032A - Cosmetic compositions comprising extracts of hubs - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a herb extract is provided to improve antioxidation, antiaging, and whitening effects and skin elasticity. CONSTITUTION: A method for preparing a herb extract comprises adding a solvent to herb selected from the group consisting of Lavandula angustifolia, Salvia officianalis L., Rosemarinus officials L., Aspalathus linearis, Ocimum basilicum L., Thymus vulgaris L., and Mentha piperita. The solvent is butylene glycol or ethanol. A cosmetic composition contains a herb extract including Lavandula angustifolia, Salvia officianalis L., Rosemarinus officials L., Aspalathus linearis, Ocimum basilicum L., Thymus vulgaris L., and Mentha piperita. [Reference numerals] (AA) Embodiment 1-8

Description

Cosmetic composition containing herb extracts {Cosmetic Compositions Comprising extracts of Hubs}

It relates to a cosmetic composition comprising an herbal extract.

Lavender ( Lavandula) angustifolia ) is a plant native to the Mediterranean coast, Somaliland, Canary Islands, India, and southern France, belonging to the family LAMIACEAE (LABIATAE).

The name Lavender comes from the Latin Lavando and comes from the verb Lavare, the word wash. Ancient Romans said that these flowers were put in the bath water to make them fragrant. In Europe, the plant has a long history of cultivation because of the charm of the fragrance. So even today, housewives in Italy have a custom of hanging clothes on lavender bushes and making the fragrance of their clothes smell. This fragrance has the effect of sterilization and insect repellent, so you can enjoy two stones. Once upon a time there were no chemical insect repellents or fragrances as it is today, it was a common custom to hang in the closet, especially the wardrobe of the old people who used lavender.

Lavender has a deeper scent when dried, and lasts longer. In the old days, the lavender scent cleared the head and rejuvenated the body, giving it vitality. Lavender perfume was applied to the forehead as a medicine for headaches, and it was used as a medicine when it fell due to epilepsy or dizziness.

In the United States, lavender flowers were used to repel insects such as mosquitoes and flies as insect repellents, and the use of lavender oil prevented mosquito bites. Lavender oil is sterilized, disinfected, and antiseptic and used for all trauma.

Essential oils extracted from lavender blossoms are called lavender oils and contain 35-58% linalyl acetate, which is one of the most important plant essential oils widely used as a cupping agent for cosmetics and soap.

Thyme ( Thymus) vulgaris L.) is a plant native to the Mediterranean Sea and Europe. Thyme, also known as musk, has a long history as a spice and medicinal plant. Thymus's scientific name is derived from the Greek thuo, or "disinfect".

Sage ( Salvia) officianalis L.) is a medicinal plant with a long history of origin in the Mediterranean Sea and southern Europe, and has long been known as a panacea.

Sage is derived from the word "healthy" or "cure," and is a famous herb from the Greek and Roman periods.

Sage medicated with dried leaves, which contain essential oils. In addition to the tonic effect, this ingredient has an excellent effect on the nervous system and digestive system. It also has antiseptic and antiseptic effects such as antiseptic, antibacterial, and anti-inflammatory, making it an excellent anti-inflammatory agent for various inflammations. It is also a panacea that also has antipyretic, gust, and thrombotic effects.

Sage strengthens the development of the brain and muscles to increase memory and, when struggling with strokes or hand and feet, washes away fatigue or pain after severe exercise.

In the tonic use example, in France, sage leaf wine was given to encourage mothers to wait for dissolution, and in Italy, sage is put on butter bread as a way to keep farmers healthy.

Sage has an excellent antiseptic effect, so there is a history that has been used as one of the health fragrances to prevent infectious diseases and odors by laying leaves on the floor.

Basil ( Ocimum basilicum L.) is a plant native to India, tropical Asia, and Africa.

Rosemary, scientific name: Rosemarinus officials L.) is an evergreen shrub that is a fragrant plant in Europe or the Mediterranean.

In ancient Israel, Greece, Egypt, and Rome, it was one of the sacred and precious spices used in religious rituals, and therefore has a long history of legends and anecdotes.

Peppermint ( Mentha) piperita ), also called spearmint, is the oldest and most in demand plant.

Due to the flavor reminiscent of pepper, the scientific name Mentha peperiata originated from the Latin piper.

It is a perennial herb that is native to Europe, grows about 30 ~ 90cm, has sharp leaves, and there are two kinds of leaves and stems that are green and purple.

Mint has long been cultivated in China and Japan, and in Egypt data about peppermint from 1000 BC before was found in the tomb. Peppermint has been used in a variety of conditions, including indigestion, nausea, laryngitis, diarrhea, headache, toothache, and abdominal pain in the East and West.

Menthol, an essential ingredient in essential oils, keeps the skin and mucous membranes cool, effective for antibacterial and pain relief, and is an edible, medicinal and fragrance in ancient Egypt, an important ingredient in perfumes in ancient Greece, and in addition to fragrances in Greece and Rome Used as an additive. It is effective for mental fatigue, depression, and nervous attacks. It cools when it is hot and warms when it is cold, which prevents the outflow of mucus and helps fever and sweating. Cold, asthma, bronchitis, cholera, pneumonia, pulmonary tuberculosis, food poisoning, neuralgia is effective.

The British Herb Pharmacy notes that it is used for stomach pain, flatulence, cold, vomiting due to pregnancy, and menstrual irregularities.

Rooibos, scientific name: Aspalathus linearis ) is a conifer belonging to the legumes. It grows only in the Cedarberg Mountains, north of Cape Town, South Africa. "Rooibos" means Afrikaans "red" (rooi) + "bos".

Dry the leaves and use them as tea. Tea is sweet, caffeine-free, extremely low in tannins, and is thought to be antioxidant. The indigenous Koisan of Cape Province have long known the effectiveness of this rooibos tea and gathered it with herbs. Dutch immigrants to Cape Province used rooibos tea as a substitute for black tea. In South Africa it is common to drink milk and sugar in rooibos as milk tea, but in many countries around the world, it is often straight. Cafes in South Africa are also popular with espresso, café latte or cappuccino.

KR 2006-0099587 The invention entitled 'Antioxidant herbal cosmetic composition for skin and scalp containing lavender, bergamot, freesia, peppermint, chamomile and rosemary, and a method of preparing the same' claims for a cosmetic composition including lavender and peppermint And KR 2008-7001207, entitled “Synergy Pharmaceutical Compositions Useful for the Prevention and Treatment of Beta-amyloid Protein Induced Diseases, Including Sage and Rosemary Inducing Compounds,” relates to pharmaceutical compositions comprising sage, rosemary inducing compounds, and the like. Although claimed, cosmetic compositions containing a combination of lavender, sage, rosemary, rooibos, basil, thyme and peppermint have not yet been disclosed.

Provided are methods for preparing lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extracts.

It provides a cosmetic composition comprising a lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract.

It provides a cosmetic composition comprising lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract as an active ingredient. The present inventors have recognized that various herbs contain various substances beneficial to the human body, and have made extensive efforts to develop a cosmetic composition including herbal extracts as an active ingredient. As a result, the cosmetic composition comprising lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract as an active ingredient has found that the antioxidant, anti-aging and whitening effect is excellent to complete the present invention.

The invention includes Lavandula angustifolia, Salvia officianalis L., Rosemarinus officials L., Aspalathus linearis, Basil (Ocimum basilicum L.), Thymus vulgaris L. and Peppermint (Mentha). It provides a method of producing a herbal extract comprising the step of obtaining an herbal extract by adding an extraction solvent to at least one or more herbs selected from the group containing piperita).

According to an embodiment of the present invention, the lavender used in the present invention may use all parts of the plant, but preferably, flowers, leaves, stems and berries may be used, and more preferably, flowers, leaves, stems may be used. Most preferably, flowers and leaves can be used.

According to an embodiment of the present invention, in the case of sage, rosemary, rooibos, basil, thyme and peppermint used in the present invention, all parts of the plant may be used, but preferably, flowers, leaves, stems, and fruits may be used. More preferably, leaves and flowers may be used, and most preferably leaves may be used.

According to an embodiment of the present invention, the herb is preferable to prepare an extract by mixing two or more of the herbs listed above, but most preferably, a herb extract by mixing lavender, sage, rosemary, rooibos, basil, thyme and peppermint To prepare.

The extraction solvent in the preparation of the herbal extract is important in that the main effect of the cosmetic composition changes by the selection of the extraction solvent as described in the following examples. According to an embodiment of the present invention, the extraction solvent used in the present invention is preferably extracted with water, a hydrophilic organic solvent or a mixed solvent thereof, more preferably with water, butylene glycol or ethanol, Most preferably it is extracted with butylene glycol or ethanol.

According to an embodiment of the present invention, when preparing a complex extract of the lavender, sage, rosemary, rooibos, basil, thyme and peppermint using butylene glycol, the antioxidant, anti-aging and whitening effect of the cosmetic composition comprising the complex extract Since it may affect the mixing ratio of the lavender, sage, rosemary, rooibos, basil, thyme and peppermint is preferably used in the mixing ratio as shown in Table 1 below, most preferably No. It is preferable to use it at the ratio of 1.

No. lavender sage Rosemary Rooibos basil time Peppermint One One One One One One One One 2 4 4 4 2 2 One One 3 8 8 8 2 2 One One 4 20 20 20 10 10 One One 5 40 40 40 10 10 One One

According to an embodiment of the present invention, when preparing a complex extract using lavender, sage, rosemary, rooibos, basil, thyme and peppermint using ethanol, the antioxidant, anti-aging and whitening effect of the cosmetic composition comprising the complex extract Since it may affect the mixing ratio of the lavender, sage, rosemary, rooibos, basil, thyme and peppermint is preferably used in the mixing ratio as shown in Table 2 below, most preferably in the ratio of No 5. It is preferable to use as.

No. Rooibos basil time lavender sage Rosemary Peppermint One One One One One One One One 2 4 4 4 2 2 2 One 3 8 8 8 2 2 2 One 4 4 4 4 One One One One 5 8 8 8 One One One One

According to another embodiment of the present invention, the present invention provides a cosmetic composition having an antioxidant, anti-aging or whitening effect, including lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract as an active ingredient.

According to an embodiment of the present invention, the content of the complex extract in the cosmetic composition is preferably 0.00002-30% by weight, more preferably 0.01-10% by weight, most preferably 0.05-4% by weight.

More details related to the manufacturing method of the lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract is related to the above-described method for producing the herbal complex extract, so the common content as described above for the excessive complexity of the present specification And the description thereof are omitted.

The cosmetic composition of the present invention may include other ingredients in addition to the aforementioned herbal extract. According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition according to the invention is excellent in antioxidant, anti-aging, skin elasticity improvement and whitening effect.

1 shows the results of SOD-like activity of Examples 1-8.
Figure 2 shows the results of the hydrogen peroxide removal effect experiment for Example 1-8.
Figure 3 shows the results of measuring melanin biosynthesis inhibitory effect on B16F1 melanoma cells for Example 4-4.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Lavender used in the present invention was cultivated at an altitude of 700 m above the Taebaek region, and the remaining six species of thyme, sage, basil, rosemary, peppermint, and rooibos are products of the French Ladrome company, and are certified by the French government and COFRAC. Products are certified and certified organic at every stage, from cultivation of material plants to final finished product production. The Organic Association (ECOCERT) was established in 1991 as an international organic certification body in France. The organization is based in Europe but is the largest organic certification body in the world, inspecting products in more than 80 countries. Only products that have passed the final inspection by carefully examining the quality of the production line and eco-friendly raw materials can be labeled by ECOCERT.

Example  1-1. lavender BG  Extract manufacturer

20 g of dry lavender was added with 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) and extracted at room temperature for 5 days. After 5 days, the herbs were filtered with a 380 mesh filter cloth and filtered with a 0.45 μm membrane filter in a filter depressurizer to obtain a final extract.

Example  1-2. sage BG Extract manufacturer

20 g of dried sage was added 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example  1-3. Rosemary BG Extract manufacturer

20 g of dried rosemary was added with 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example 1-4. Rooibos BG extract manufacturing

To 20 g of dried rooibos, 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) was added thereto, and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example  1-5. basil BG Extract manufacturer

20 g of dried basil outpost was added with 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example  1-6. time BG Extract manufacturer

To 20 g of dried time, 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) was added thereto, and extracted at room temperature for 5 days. After 5 days, the herbs were filtered with a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter, and then the final extract was obtained.

Example  1-7. Peppermint BG Extract manufacturer

20 g of dried peppermint was added with 500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) and extracted at room temperature for 5 days. After 5 days, the herbs were filtered with a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Examples 1-8. Herb Complex A. BG Extract Preparation

To 35 g of the same mixture of dried lavender, thyme, sage, basil, rosemary, peppermint, and rooibos, add 700 g of 30% butylene glycol (sample: solvent = 1: 20 wt / wt), and keep at room temperature for 5 days. Extracted daily. Thereafter, the herbs were filtered with a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter, and then the final extract was obtained.

Example 2-1. Lavender Ethanol Extract Manufacturer

100 g of dry lavender was added 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Example 2-2. Sage Ethanol Extract Manufacturer

100 g of dried sage was added 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Examples 2-3. Rosemary Ethanol Extract Manufacturer

100 g of dried rosemary was added 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt), and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Examples 2-4. Rooibos Ethanol Extract Preparation

100 g of dried rooibos was added 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Examples 2-5. Basil Ethanol Extract Manufacturer

To 100 g of dried basil, 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added thereto, and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Examples 2-6. Thyme ethanol extract manufacturer

100 g of dried time was added 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Example 2-7. Peppermint Ethanol Extract Preparation

To 100 g of dried peppermint, 1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added thereto, and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Examples 2-8. Herb Complex A Ethanol Extract Preparation

15 g of dried lavender, thyme, sage, basil, rosemary, peppermint and rooibos were mixed with 105 g of 1575 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) and extracted at room temperature for 3 days. . The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Example  3-1. Herb complex B. BG  Extract manufacturer

900 g of 30% butylene glycol (sample: solvent = 1: 20) in 45 g of a mixture of herbs including dried lavender, sage, 10 g of rosemary, 5 g of rooibos, 5 g of basil, 2.5 g of thyme, 2.5 g of peppermint wt / wt) was added and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example 3-2. Herb Complex C. BG Extract Preparation

20 g of dried lavender, sage, 20 g of rosemary, 5 g of rooibos, 5 g of basil, 2.5 g of thyme, 2.5 g of peppermint, 1500 g of 30% butylene glycol (sample: solvent = 1: 20) wt / wt) was added and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example 3-3. Herb Complex D. BG Extract Preparation

820 g of 30% butylene glycol (sample: solvent = 1: 20) in 41 g of a mixture of herbs including dried lavender, sage, 10 g of rosemary, 5 g of rooibos, 5 g of basil, 0.5 g of thyme, 0.5 g of peppermint wt / wt) was added and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example 3-4. Herb Complex E. BG Extract Preparation

1420 g of 30% butylene glycol (sample: solvent = 1: 71 g) in a mixture of herbs including 20 g of dried lavender, sage, rosemary, 20 g of rooibos, 5 g of basil, 0.5 g of thyme and 0.5 g of peppermint. 20 wt / wt) was added and extracted at room temperature for 5 days. After 5 days, the herbs were filtered through a 380 mesh filter cloth, filtered with a 0.45 μm membrane filter and filtered to obtain a final extract.

Example 4-1. Herb Complex B Ethanol Extract Preparation

142.5 g of 2137.5 g of 70% ethanol (sample: solvent = 1: 15 wt) in a mixture of herbs such as dried rooibos, basil, thyme 30 g, 15 g lavender, 15 g sage, 15 g rosemary, 7.5 g peppermint / wt) was added and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Example 4-2. Herb Complex C Ethanol Extract Manufacturer

In a mixture of herbs including dried rooibos, basil, thyme 60 g, lavender, sage, rosemary 15 g each, peppermint 7.5 g and 232.5 g 70% 3487.5 g (sample: solvent = 1: 15 wt / wt) Was added and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Example 4-3. Herb Complex D Ethanol Extract Manufacturer

1800 g of 70% ethanol 1800 g (sample: solvent = 1: 15 wt / wt) in a mixture of herbs including dried rooibos, basil, thyme 30 g, lavender, sage, rosemary 7.5 g, peppermint 7.5 g Put in and extract at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol, and extracted at room temperature for 3 days. The final extract was obtained by lyophilization.

Example 4-4. Herb Complex E Ethanol Extract Manufacturer

3150 g of 70% ethanol 3150 g (sample: solvent = 1: 15 wt / wt) in a mixture of herbs including dried rooibos, basil, thyme 60 g, lavender, sage, rosemary 7.5 g and peppermint 7.5 g Was added and extracted at room temperature for 3 days. The herbs were filtered through a 380 mesh filter cloth and the filtrates were collected. The filtered herbs were added to the same amount of 70% ethanol and extracted at room temperature for 3 days, the herbs were filtered with a 380 mesh filter cloth, and the filtrate was collected. Finally, the filtered herbs were extracted with the same amount of 70% ethanol at room temperature for 3 days, filtered with 380 mesh filter cloth, filtered with a filtrate and a 0.45 ㎛ membrane filter together with the filtrate collected above. The final extract was obtained by lyophilization.

Experimental Example 1. Cytotoxicity Test

Human fibroblast cells were cultured in 10% FBS-IMDM (Iscove's Modified Dulbecco's Medium, Gibco), and then taken to prepare a cell solution in which the cell concentration was adjusted to 5 × 104 cells / ml, followed by 96 well plate 20 μl was dispensed into the flask, which was incubated for 24 hours in a 37 ° C., 5% CO 2 incubator. After 24 hours of incubation, the medium was removed, and 200 μl of the extract according to the concentrations of Examples 1-1 to 4-4 prepared with 10% FBS-IMDM and 180 μl of FBS-free IMDM were added again to react for 48 hours under the same conditions. I was. At this time, the experiment using only FBS-free IMDM was used as a control of the sample. 3 hours before the completion of the reaction, 20 μl of 5 mg / ml MTT solution (in 1 × PBS (−)) was added, followed by further reaction for 48 hours. After removing the MTT reaction solution, 150 μl of DMSO (dimethyl sulfoxide) was added thereto, followed by stirring for 5 minutes (room temperature), and the absorbance was measured at 570 nm using a microplate counter (Bio-Rad, USA).

Cytotoxicity Evaluation Results of Example 1 Cell proliferation (%) Samples (占 퐂 / ml) Control 4 2 One 0.5 Example 1-1 100 221.33 156.01 152.24 124.27 Examples 1-2 100 218.19 211.06 159.09 148.77 Example 1-3 100 190.78 112.24 124.25 118.50 Example 1-4 100 124.41 108.99 102.41 112.48 Examples 1-5 100 135.38 111.82 116.93 124.81 Examples 1-6 100 189.69 177.02 187.81 121.65 Example 1-7 100 201.48 199.24 190.26 169.45 Examples 1-8 100 209.43 195.59 168.25 153.57

As a result of the dermal fibroblast cytotoxicity test for Example 1, it was found that there is no toxicity in all the test zones, rather, it was found that the effect of promoting cell proliferation on the dermal dermal fibroblast cells in a concentration-dependent manner.

Cytotoxicity Evaluation Results of Example 2 Cell proliferation (%) Samples (占 퐂 / ml) Control 200 150 100 50 Example 2-1 100 24.99 25.88 64.51 143.95 Example 2-2 100 32.35 28.02 71.78 109.56 Example 2-3 100 44.27 51.08 82.49 100.85 Examples 2-4 100 162.30 122.42 110.83 95.74 Example 2-5 100 103.12 108.89 112.54 83.92 Examples 2-6 100 134.39 122.33 144.70 121.85 Examples 2-7 100 103.23 94.96 95.63 98.05 Examples 2-8 100 43.33 110.94 122.14 136.47

As a result of skin fibroblast cytotoxicity test for Example 2, in the case of Examples 2-1, 2-2 and 2-3, the concentration of 100 μg / ml and the composite extract of Example 2-8 was 200 μg / ml or more It showed cytotoxicity in and the other examples did not show cytotoxicity. Therefore, in the case of 70% ethanol extract of Example 2, it was determined that the raptor, sage, and rosemary were toxic, and then proceeded to reflect this when manufacturing the composite.

Cytotoxicity Evaluation Results of Examples 1-8 and 3 Cell proliferation (%) Samples (占 퐂 / ml) Control 4 2 One 0.5 Examples 1-8 100 209.43 195.59 168.25 153.57 Example 3-1 100 196.07 156.58 138.90 129.08 Example 3-2 100 163.37 130.73 109.41 100.42 Example 3-3 100 164.50 144.31 127.34 104.53 Example 3-4 100 179.79 137.27 134.67 106.37

As a result of dermal fibroblast cytotoxicity test on the composite extracts of Examples 1-8 and 3, all the test cells showed no cytotoxicity and all showed excellent skin cell proliferation effect. It showed the highest proliferative effect.

Cytotoxicity Evaluation Results of Examples 2-8 and 4 Cell proliferation (%) Samples (占 퐂 / ml) Control 200 150 100 50 Examples 2-8 100 43.33 110.94 122.14 136.47 Example 4-1 100 115.71 90.79 102.48 99.27 Example 4-2 100 86.82 100.25 101.89 105.47 Example 4-3 100 116.90 103.35 95.27 103.30 Example 4-4 100 104.68 102.37 98.49 112.47

As a result of skin fibroblast cytotoxicity test on the composite extracts of Examples 1-8 and 3, it was found that the cytotoxicity shown in Examples 1-8 was not shown in Example 4.

Experimental Example  2. Antioxidant Experiment

2-1. DPPH assay

100 μl of 5 × 10-4M DPPH (1-1-diphenyl-2-picryl-hydrazyl) was added to 100 μl of the concentration-specific solutions of all the examples, stirred for 1 minute, and then reacted in a 25 ° C. incubator for 30 minutes. Absorbance was measured at 540 nm using a microplate counter. L-ascorbic acid (100µg / ml) was used as the experimental control, and the electron donating ability was expressed as the absorbance decrease rate of the addition and no addition of the sample solution.

Antioxidant Experiment Results of Example 1 30% B.G Antioxidant Effect (%) sample (%) Control 4 2 One 0.5 Example 1-1 94 76 62 36 39 Examples 1-2 94 80 69 34 14 Example 1-3 95 75 36 17 15 Example 1-4 95 82 53 24 21 Examples 1-5 95 82 45 27 11 Examples 1-6 93 82 55 29 12 Example 1-7 95 68 39 11 8 Examples 1-8 94 89 73 56 39

In all the examples showed a significant DPPH scavenging capacity of 0.5-4% concentration, it was found that the antioxidant activity is high.

Antioxidant Experiment Results of Example 2 DPPH Scavenging Power (%) Samples (占 퐂 / ml) Control 1000 100 10 Example 2-1 96 60 -4 7 Example 2-2 93 82 17 One Example 2-3 93 77 10 2 Examples 2-4 93 81 25 5 Example 2-5 93 46 6 11 Examples 2-6 96 82 18 2 Examples 2-7 96 83 26 4 Examples 2-8 94 81 50 12

In all the examples showed a significant DPPH scavenging ability of each concentration of 10-1,000 ㎍ / ㎖ showed a high antioxidant activity.

Results of Antioxidant Experiments of Examples 1-8 and 3 DPPH Scavenging Power (%) sample (%) Control 4 2 One 0.5 Examples 1-8 94 89 73 56 39 Example 3-1 94 85 58 27 14 Example 3-2 95 77 45 25 15 Example 3-3 95 81 46 28 21 Example 3-4 95 65 38 19 11

All the test plots showed high DPPH scavenging ability and there was no significant difference between the examples, but Examples 1-8 showed a slightly higher trend.

Results of Antioxidant Experiments of Examples 2-8 and 4 DPPH Scavenging Power (%) Samples (占 퐂 / ml) Control 1000 100 10 Examples 2-8 94 81 50 12 Example 4-1 94 82 48 5 Example 4-2 95 77 47 4 Example 4-3 95 81 43 6 Example 4-4 95 46 46 5

All the test plots showed high DPPH scavenging ability and there was no significant difference between the examples.

2-2. Superoxide dismutase (SOD) -like activity test

SOD-like activity was measured for Example 1-8, which showed the best effect in the results of Experimental Example 2-1. After adding 200 μl of WST working solution in SOD assay kit (WST) to 20 μl of sample solution for each concentration, and adding 20 μl of enzyme working solution again after mixing, The reaction was carried out for 20 minutes in an incubator at 37 ° C., and the absorbance was measured at 450 nm using a microplate counter. Peroxide dismutase (100 units, Sigma) was used as an experimental control, and peroxide dismutase-like activity was indicated by the absorbance reduction rate of the added and non-added samples. As a result of the measurement, it was found that in the case of Example 1-8 has an excellent SOD-like activity (see Fig. 1).

2-3. Removal effect of hydrogen peroxide

In Example 1-8, which showed the best effect in the results of Experimental Example 2-1, a hydrogen peroxide removal effect experiment was performed as follows. Hydrogen peroxide removal effect experiment was carried out by adding 20 μl of sample solution for each concentration, 20 μl of 10 mM hydrogen peroxide, 100 μl of 0.1 M phosphate buffer (pH 5) and reacting for 5 minutes at 37 ° C., followed by 1.25 mM ABTS and 1 U / ml per. 30 μl of Roxydays was added and reacted at 37 ° C. for 10 minutes, and the absorbance was measured at 405 nm. As a result of the hydrogen peroxide removal effect experiment results for Example 1-8, it was obtained a significant result of the non-peroxide, it was determined that the antioxidant activity according to the hydrogen peroxide removal effect is excellent (see Fig. 2).

Experimental Example  3. Whitening Experiment

3-1. Inhibitory effect of melanin production using mushroom tyrosinase

Measurement of tyrosinase activity inhibitory activity for Examples 1 to 4 was measured by modifying a method such as Yagi, which measures dopa chrome produced by the action of tyrosinase by colorimetry. That is, the reaction sphere was added 40 μl of 1.5 mM L-tyrosine (in 0.1 M phosphate buffer (pH 6.5)) and 10 μl of sample solution to 110 μl of 0.1 M phosphate buffer (pH6.5) in a 96 well plate, followed by mushroom tyrosinase. 10 µl (2000 unit / ml) was added thereto and reacted at 37 ° C for 10 minutes, and the dopachrome produced in the reaction solution was measured at 490 nm using a microplate counter. In addition, arbutin (500 μg / ml) was used as a positive control for comparing the test results.

Inhibitory effect of tyrosinase activity of Example 1 Tyrosinase activity inhibitory effect (%) sample (%) Control 10 One 0.1 Example 1-1 41 3 -26 -7 Examples 1-2 41 15 -11 -12 Example 1-3 44 9 3 2 Example 1-4 44 4 3 3 Examples 1-5 49 11 3 2 Examples 1-6 49 4 One One Example 1-7 49 2 2 3 Examples 1-8 44 9 3 3

Inhibitory effect of tyrosinase activity of Example 2 Tyrosinase activity inhibitory effect (%) Samples (占 퐂 / ml) Control 100 Example 2-1 41 19 Example 2-2 41 26 Example 2-3 44 7 Examples 2-4 44 7 Example 2-5 49 16 Examples 2-6 49 24 Examples 2-7 49 10 Examples 2-8 44 9

Inhibitory Effect of Tyrosinase Activity of Examples 1-8 and 3 Tyrosinase activity inhibitory effect (%) sample (%) Control 10 One 0.1 Examples 1-8 43 9 3 3 Example 3-1 43 16 9 One Example 3-2 43 15 8 One Example 3-3 44 7 3 One Example 3-4 49 14 6 3

Inhibitory Effect of Tyrosinase Activity of Examples 2-8 and 4 Tyrosinase activity inhibitory effect (%) Samples (占 퐂 / ml) Control 1000 500 100 50 Examples 2-8 47 37 28 9 3 Example 4-1 41 41 28 9 4 Example 4-2 50 50 31 10 One Example 4-3 50 38 24 6 6 Example 4-4 49 53 36 6 0

BG extracts of Examples 1 and 3 did not show a tyrosinase activity inhibitory effect, but Examples 2 and 4 showed activity inhibitory effects by concentration, and also showed excellent activity in the results of Examples 2-8 and 4 of the composite extract. In particular, Examples 4-4 showed the most excellent effect, and tested the inhibitory effect of intracellular melanin biosynthesis.

3-2. Cytotoxicity Tests for B16 Melanoma

In Example 4-4, which showed the best effect in the results of Experiment 3-1, the toxicity test for B16F1 melanoma cells was performed as follows. Cytotoxicity test for mouse-derived B16F1 melanoma proceeded as follows. That is, after incubating B16F1 (mouse melanoma) in 10% FBS-DMEM, the cell solution was adjusted to adjust the cell concentration to 5 × 10 4 cells / mL, and then 200 μl of this was mixed in a 96 well plate and 37 C was incubated for 24 hours in a 5% CO 2 incubator. After incubation for 24 hours, the medium was removed, and then 20 µl of the sample prepared for each concentration and 180 µl of 10% FBS-DMEM were added again, and then cultured under the same conditions for 7 days. At this time, the experiment using only 10% FBS-DMEM was used as a control. After incubation for 3 days, the medium and the solution were removed, 200 μl of 50 μg / ml neutral red (10% FBS-DMEM) was added thereto, and the reaction was carried out for 2 hours in a 37 ° C. incubator. After removing the neutral red solution, add 100 μl of 1% CaCl 2, 1% formaldehyde (inD.W) solution and stir for 1 minute, and then remove the solution and add 1% acetic acid and 50% EtOH (in DW). After 100 μl each and stirring for 15 minutes, the absorbance was measured at 570 nm using a microplate reader.

Toxic Effects on Melanoma Cells of Examples 4-4 Cell proliferation (%) Samples (占 퐂 / ml) Control 100 10 One Example 4-4 100 102.49 101.65 102.18

Example 4-4 showed no toxicity to B16F1 melanoma cells as a result of the test. Intracellular melanin biosynthesis inhibitory effect was measured based on the maximum concentration of 100 μg / ml.

3-3. Suppresses melanin biosynthesis in B16 melanoma

The effect of inhibiting melanin biosynthesis in B16F1 melanoma cells for Examples 4-4 was measured. In order to determine the effect of inhibiting melanin (melanin) biosynthesis in the mouse-derived B16F1 melanoma (melanoma) was carried out as follows. That is, after culturing B16F1 (melanoma) in 10% FBS-DMEM to prepare a cell solution adjusted to a concentration of 5.0 × 104 cells / ㎖ by taking this, and dispensed 2 ml in 6 well plate, 37 ℃ Incubated for 24 hours in a 5% CO 2 incubator. After incubation for 24 hours, after removing the medium, 1.8 mL of the medium prepared with a final concentration of 0.2 uM α-MSH and 200 μl of the sample for each concentration were mixed, and then cultured again under the same conditions for 72 hours. In the case of blanks, only medium without α-MSH was added, and the same concentration of arbutin was used as the positive control. After incubation for 72 hours, the plate was washed twice with 1 × PBS (-) to measure the amount of melanin in solution in each plate. 200 μl of trypsin-EDTA solution was added to the cells, and the cells were separated. The supernatant was removed. The separated pellet was washed twice with 1 × PBS (-), and then dissolved in 400 μl of 10% DMSO (in 1M NaOH), followed by 200 μl of the pellet, which was dispensed into a 96 well plate, using a microplate counter. Absorbance was measured at 405 nm. As a result of measuring melanin biosynthesis inhibitory effect on B16F1 melanoma cells for Example 4-4, it showed a concentration-dependent melanin biosynthesis inhibitory effect at a concentration of 25 ~ 100㎍ / ㎖. It will be used to develop new whitening cosmetics using this.

The following shows an example of the cosmetic composition as a formulation containing the present mixed extract in each cosmetic formulation to remove free radicals in the skin, protect the skin, and improve skin elasticity and whitening. . The extracts used in the following formulations are of Examples 1-8 and 4-4.

Prescription example  One

Prescription examples of the flexible cosmetic water in the cosmetics containing the extracts of Examples 1-8 and 4-4 are as follows.

Formulation example of softening longevity containing extracts of Examples 1-8 and 4-4 ingredient Content (% by weight) Examples 1-8 2 Example 4-4 0.01 Polyoxyethylene Cured Castor Oil 0.5 Glycine 3.0 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 2

Prescription examples of nutritional longevity in cosmetics containing the extracts of Examples 1-8 and 4-4 are as follows.

Formulation example of nutritional cosmetics containing extracts of Examples 1-8 and 4-4 ingredient Content (% by weight) Examples 1-8 2 Example 4-4 0.01 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 3

Prescription examples of essences in the cosmetics containing the extracts of Examples 1-8 and 4-4 are as follows.

Essence formulation example containing extracts of Examples 1-8 and 4-4 ingredient Content (% by weight) Examples 1-8 4 Example 4-4 0.01 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaluronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene Cured Castor Oil 1.0 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription example  4

Prescription examples of creams in the cosmetic preparations containing the extracts of Examples 1-8 and 4-4 are as follows.

Cream formulation containing extracts of Examples 1-8 and 4-4 ingredient Content (% by weight) Examples 1-8 4 Example 4-4 0.01 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription example  5

Prescription examples of packs in cosmetics containing extracts of Examples 1-8 and 4-4 are as follows.

Prescription pack containing extracts of Examples 1-8 and 4-4 ingredient Content (% by weight) Examples 1-8 4 Example 4-4 0.01 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyaluronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

The following are examples of skin elasticity, whitening, and skin improvement of cosmetic formulations containing an optimal blend of lavender, sage, rosemary, rooibos, basil, thyme, and peppermint through human application tests. The prescription example used herein is the cream formulation of Formulation Example 4.

Experimental Example 4.

4-1. Skin elasticity improvement test

In order to confirm that the antioxidant effect shown in the in-vitro experiment actually helps to improve skin elasticity in humans, human application tests were performed on creams containing Examples 1-8 and 4-4 (prescription example 4). . The skin elasticity test using the Cutometer SEM 575 device occurs when the skin is pulled and returned to its original state, that is, when the surface of the skin is sucked at a certain pressure and then returned to the skin momentarily after a certain time. The elongation and restoration of the skin was measured to measure the efficacy (value of elasticity). The test consisted of 10 subjects (5 control and 5 test subjects), all of whom were female, with an average age of 34 years. Test conditions were 350 mbar. The test was repeated three times under vacuum on (2 sec) and vacuum off (2 sec) conditions. After the probe was attached to the center of the ball of the subject, the degree of skin deformation was measured. In this way, the average value was determined by measuring five times a week, two weeks, four weeks, and six weeks before use. . All tests were conducted in a constant temperature and humidity room with 20-24 ℃ and 45-55% humidity, and after washing, they were acclimated for at least 1 hour in a constant temperature and humidity room. The measured test value was calculated as follows to calculate the elasticity.

Skin elasticity improvement test of the cream containing extracts of Examples 1-8 and 4-4 Before use 1 week 2 weeks 4 weeks 6 weeks Control Test Control Test Control Test Control Test Control Test R2 0.75 0.78 0.76 0.81 0.78 0.85 0.79 0.89 0.78 0.91

-Uf: When pulling out the skin (highest point)

-Ue: When the skin is pulled out (lowest point)

Total resilience = Ue / Uf (R2)

The total elasticity is closer to "1", the more elastic skin

Improvement of skin elasticity for Formulation Example 4 As a result of the human application test, there was a significant difference between the test and the control group, and it was found that the effect of improving the elasticity was started from about 2 weeks.

4-2. Skin whitening effect test

In order to confirm that the whitening effect shown in the in-vitro experiment actually helps human skin whitening, a human application test was conducted on the creams containing Examples 1-8 and 4-4. The skin whitening test using the Roboskin analyzer equipment periodically evaluated the improvement of pigmentation and skin tone (brightness) while applying a sample or a product to the subject's face. The test was also performed in the same manner as the skin elasticity improvement test, and therefore, 10 subjects (5 control and 5 test subjects) were conducted. Also, the average value was obtained by measuring five times before, after 1, 2, 4, and 6 weeks of use. It was measured after adjusting for at least 1 hour in a humidity room.

Skin whitening effect test of cream containing extracts of Examples 1-8 and 4-4 Before use 1 week 2 weeks 4 weeks 6 weeks Control Test Control Test Control Test Control Test Control Test Pigmentation (small) 9.3 9.0 9.2 8.5 9.0 7.6 8.7 6.2 8.3 4.3 Pigmentation (large) 32.0 31.5 31.8 30.7 31.1 29.8 29.6 27.5 28.8 23.9 brightness 65.5 66.0 66.0 68.0 66.2 71.8 67.8 75.4 69.7 77.9

As a result of measuring the skin whitening effect of Formulation Example 4, in the case of Formulation Example 4, pigmentation (large and small) also tended to decrease from the 2nd week, and the brightness of the skin was also brightened, which helped skin whitening. I could see that.

Claims (11)

Lavandula angustifolia ), sage ( Salvia officianalis L., Rosemarinus officials L.), Aspalathus linearis , ocimum basilicum L., thyme ( Thymus vulgaris L.) and peppermint ( Mentha piperita ), the method comprising the step of adding an extracting solvent to the at least one herb selected from the group comprising a herb extract Method of Preparation of Extracts.
The method of claim 1, wherein the herbs are lavender, sage, rosemary, rooibos, basil, thyme and peppermint.
The method of claim 2, wherein the extraction solvent is a butylene glycol (Butylene glycol) method for producing a herb extract, characterized in that.
The method of claim 2, wherein the extractant is ethanol (ethanol).
The mixing ratio of the lavender, sage, rosemary, rooibos, basil, thyme and peppermint is 1: 1: 1: 1: 1: 1: 4: 4: 4: 2: 2: 1: 1 , 8: 8: 8: 2: 2: 1: 1, 20: 20: 20: 10: 10: 1: 1 and 40: 40: 40: 10: 10: 1: 1 Method of producing an herbal extract.
The mixing ratio of the rooibos, basil, thyme, lavender, sage, rosemary and peppermint is 1: 1: 1: 1: 1: 1, 4: 4: 4: 2: 2: 2: 1, 8: 8: 8: 2: 2: 2: 1, 4: 4: 4: 1: 1: 1: 1 and 8: 8: 8: 1: 1: 1: 1 selected from the group containing. Method for producing a herb extract, characterized in that.
A cosmetic composition having an antioxidant, anti-aging or whitening effect, comprising an herbal extract prepared by the method of any one of claims 1 to 6 as an active ingredient.
A cosmetic composition having an antioxidant, anti-aging or whitening effect comprising lavender, sage, rosemary, rooibos, basil, thyme and peppermint complex extract as an active ingredient.
The cosmetic composition according to claim 8, wherein the content of the complex extract is 0.00002-30% by weight.
10. The cosmetic composition according to any one of claims 8 to 9, wherein the cosmetic composition has a formulation of softening cream, nutrient cream, essence, cream or pack.
9. The cosmetic composition of claim 8, wherein the cosmetic composition is at least one selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hardened castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin A composition further comprising an auxiliary component.

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