KR101521754B1 - Cosmetic composition having antioxidation activity - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antioxidative activity, and more particularly to a cosmetic composition having an antioxidative activity comprising a fermented extract of Sasa as an active ingredient. The cosmetic composition having an antioxidative activity according to the present invention is excellent in skin-aging and antioxidative effect and persistence of effect, while being safe for skin.

Description

[0001] Cosmetic composition having antioxidation activity [0002]

The present invention relates to a cosmetic composition having antioxidant activity, and more particularly, to a cosmetic composition having antioxidant activity that is safe to the skin and has excellent anti-aging and antioxidant effects and long-lasting effects.

In general, there are many ways to explain skin aging, but the free radical theory that skin is aged by free radicals caused by various causes outside the body is accepted as the most valid theory. Free radicals are highly chemically reactive chemicals that are produced in the body by external factors such as ultraviolet radiation, radiation, exhaust gas, tobacco smoke, stress, and metabolism of mitochondria and leukocytes in the body , Which leads to the peroxidation of the lipid component of the cell membrane through a multistage chain reaction.

The major part of free radicals related to skin aging is reactive oxygen species (ROS), which include superoxide radicals, hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals (ROS) , Singlet oxygen (singlet oxygen), and the like. The lipid peroxidation caused by these causes various active oxygen species of radicals, peroxides, aldehydes and epoxides, damaging DNA, RNA, proteins, cell membranes and cell structure.

These reactive oxygen species are thought to be the main cause of cancer, tissue damage, and aging (Black HS, Photochemphotobiol. 46 (2): 213 (1987)). For example, capillary peroxidation, which constitutes a cell, can be characterized by changes in structure and fluidity of the cell membrane, increased permeability of cytoplasmic material, leakage of lyceosomal enzyme, inactivation of membrane enzymes and transport proteins, Chain cleavage, DNA damage, and mutation. Among these reactions, peroxidation of skin constitutive membranes and modification of dendritic proteins are known to be most closely related to skin aging.

Aerobic organisms have protective mechanisms to prevent the production of these oxidants and the lipid peroxidation. These protective mechanisms include enzymes such as superoxide dismutase (SOD) and catalase, tocopherol (vitamin E), beta- carotene, ascorbic acid (vitamin C) And lipid soluble and water soluble antioxidants or radical scavengers such as glutathione.

On the other hand, skin antioxidants known so far have been developed in anticipation of the following effects. First, it provides moisture to the skin, second, antioxidant effect, third, promotes the proliferation of fibroblasts involved in the activation of skin cells, and fourth, an enzyme that accelerates the production of wrinkles and loss of elasticity by decomposing collagen and elastin, Collagenase and elastase, and fifth, promotion of collagen and elastase synthesis.

As described above, since free radical oxygen or reactive oxygen species are highly related to skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, and flavonoids have been added to cosmetics There have been a lot of efforts to achieve the skin aging prevention effect by mixing the above ingredients. However, when the above ingredients are mixed in cosmetics, safety, stability, and the like are poor, and it is difficult to expect a substantial effect.

Conventionally, a substance such as BHT (2,6-di-tertiarybutyl-4-methylphenol), BHA (2-tertbutyl-4-methoxyphenol), or PG (3,4,5-terhydroxy benzoic acid propyl ester) Used a lot. However, there are disadvantages such as consumers' rejection of such synthetic antioxidants as their diet and living standards are improved. In particular, BHT, which is the most widely used synthetic antioxidant in the past, has been reported to cause cancer when consumed at 50 mg / kg / day or more. Therefore, although researches to find a substance having antioxidant activity from natural materials such as shim products were carried out vigorously, most of them had antioxidant ability which did not meet the expectation.

Therefore, in order to solve the problems of the prior art described above, there is a continuing need for the development of products and raw materials having superior ability to remove active oxygen.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

SUMMARY OF THE INVENTION Accordingly,

More specifically, the present invention provides a cosmetic composition having antioxidative activity, which is safe to the skin and has excellent anti-aging and antioxidative effects on the skin and persistence of the effect.

Another object of the present invention is to provide a cosmetic composition having an antioxidative activity that further enhances the pharmacological effect of each medicinal substance through fermentation and further enhances collagen synthesis promoting effect.

In order to accomplish the above object, the present invention relates to a method for producing a cucumber seedlings, comprising the steps of: preparing a mixture of liquorice, cinnamon, gobon, ginseng, gugija, mung bean, jujube, fungus, cordyceps, plum, The present invention provides a cosmetic composition having an antioxidant activity as an active ingredient, which comprises a fermented extract of a medicinal substance selected from the group consisting of wheat germ oil, ginseng, ginseng, yam, chrysanthemum, ginger, gardenia, golden ginseng and ginseng root.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

The cosmetic composition having an antioxidative activity according to the present invention is excellent in skin-aging and antioxidative effect and persistence of effect, while being safe for skin.

Hereinafter, the cosmetic composition having the antioxidative activity of the present invention will be described in more detail.

The medicinal plant extracts described in the present invention can be used as a medicinal herb that is used in the present invention as a medicinal plant such as licorice, cinnamon, gobon, rosemary, gugija, mung bean, jujube, fungus, cordyceps, plum, Refers to an extract obtained by extracting one or more kinds of medicinal materials selected from the group consisting of ginseng, ginseng, yam, chestnut, chrysanthemum, gardenia, seaweed, gold and ginseng, according to a conventional method. Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The present inventors have conducted studies to develop a fermented Chinese medicinal herb having excellent antioxidant function and have found that it is possible to produce a fermented Chinese medicinal herb which has excellent antioxidant function and can be used as a medicinal herb such as licorice, cinnamon, gobon, ginseng, gugija, mung bean, The fermentation broths such as Seesin, Horse riding, Shinyi, Wormwood, Woosu, Ulgum, Ginseng, Yams, Chuncheon, Chunghwa powder, Gardenia, Seaweed, Golden ginseng, and Ginseng Cordyceps were found to have superior antioxidative effects compared to the respective extracts. Thereby completing the invention.

The cosmetic composition having antioxidant activity according to the present invention is useful as an antioxidant for cosmetic compositions having an antioxidant activity and an antioxidant activity. As an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of wheat germ, ginseng, ginseng, yam, chrysanthemum, ginger, ginseng, golden ginseng, and ginseng ginseng.

The licorice (Glycyrrhiza glabra) is a herbaceous perennial herb, and has long been used as a licorice in all prescriptions as a herbal medicine for the poisoning of licorice. Licorice is a medicine to poison the drug as well as mildly prescription drugs, and it also acts to dull the drug (the main herbarium). Licorice ricusacid ginseng action, anti-inflammatory action, anti-ulcer action can treat gastroduodenal ulcers and the like.

Cinnamomum cassia (Cinnamomum cassia) is a blue-arboreal tree with camphor tree, and its shell is dried. I have been used as a medicine to keep warm sweating, heat depression medicine, analgesic medicine and Huhan from ancient times. Cinnamon is said to stop sweating, and it is said that there is an effect that releases fever and relieves aches and pains. It is used as a directional sweetener, as a raw material for the production of cinnamon oil, and as a fragrance for foodstuffs.

The textbook (Angelica tenuissima) is a perennial plant of the mountain type with a height of about 30 to 80 cm, and has no hair on the whole and strong fragrance. There is another name: Stone Echo, Winemaker. Producers contain procumarin and essential oils, the main components of which are 3-butyl phthalide and cndilide. The roots contain 1.5% essential oil. Announced, the effect of a giant-bell jets. It is used for headache, two orthodontic pain, mucus abdominal pain, diarrhea, addiction, menstrual cramps, headache mellitus, bone pain and upper limit of spring and summer, and headache.

The gosam (Sophora angustifolia) is a perennial herb that is about 1 m high with soybean. The shape of the leaves is similar to that of painting trees, which have a nickname of massive, massive, and deadly. In autumn, the root is caught, washed and dried. It tastes good, but it has the same effect as ginseng. It is used for placebo, diuretic, antipyretic, analgesic, insecticide, jaundice, dysuria, omega, eczema, and dry remedy. It is said to be a good medicine if you take it for a long time. It also has the effect of increasing the sexual function of women. It is used as a palliative drug, phlegm drug, bronchitis, and pulmonary tuberculosis. When treating malignant tumors, use dalyeoseo. It is also used for jaundice, febrile diseases and epilepsy, and also for headache, rheumatism and sinusitis. Root plaster is used for trichomoniasis vaginitis, eczema, and neurotic dermatitis. In the private sector, it is used as a pesticide for dermatological pesticides or as a pesticide for agricultural crops.

The Lycium chinese is a vine shrub with a length of 4 m. The fruit of this plant is called gojyugi, and its root is called gigolgipi. Call the stem shell. It can be used to improve liver function and prevent arteriosclerosis. The better the eyes are, the better the liver is, and the better the eyes are. If you eat for a long time to strengthen your bones, lighten your body, make your hair gray, brighten your eyes, and live longer without getting cold and heat. It is said that it can be difficult to eat young bachelor because it is very strong to cause the two companies to mix gugija and corn oil. Gigobia is used as a tonic, and it can also be used to soak gagokpi. When dioscorea is extracted by collecting leaf leaves from leaves and pruning, it is more effective than a therapeutic agent for arteriosclerosis caused by feces.

The mung bean (Phaseolus radiatus) is an annual herb of soybean and has a height of 30 to 60 cm. Leaves are compound leaves with four ovate leaflets. Diuretics, antipyretic drugs, detoxifying drugs, etc., alone, sub-drug, etc.

The jujube (Zizyphus jujube) is said to be buds and is called 夏 芽 in Japan. The fruit extract has diuretic effect and blood pressure lowering effect in hypertensive patients. Inorganic salts help to heal anemia. Jujube has an anti-allergic component, which is good for bronchial asthma, endocrine diseases and psychosis. Jujube has been used as an antidote, antipyretic, and as a nystagmus for treating anxiety, and used as an antidote. Tonic medicine, cough, 곪 long, when the whole body is sick, irritable purulent efficacy is also effective.

Aralia continentalis is a perennial herbaceous plant of the Acanthopanax sp. Prior to the 10th century, it was known that the name of virulence was called ganghwam, and that the originals of ganghwam and virginity were confused and practically used the same medicinal substance. However, after the 10th century, ganghwam and simplicity were used separately. There is data that the effect of penis is more effective than that of ganghwam, but it is effective because it does not have effect of sending out wind and it uses ganghwam and hyeolmyeong together. In Korea and Japan, it uses roots as a habit, and in China, it uses the roots of the plants in the body herbs of the buttercups as a souvenir. Sweating, windblownness, analgesic medicine, arthritis, cold, swelling, headache, toothache, neuralgia, rheumatism, itchy skin is used. It is also used for spinal neuromyositis (sciatica).

Cordyceps sinensis is a fungus belonging to the order Lycopersia. It enters the larva or silkworm of insects (Hepialus armoricanus Oberthur belonging to Lynx) during winter and grows in it and sclerotia. In the summer, Length 3 ~ 4 ㎝) grows. It looks like insects in winter, but in summer it is called caterpillar fungus because of its appearance of grass-like fruiting bodies. Dead larvae and fruiting bodies attached to them are used together as a medicine. The water extract significantly expands the bronchial tubes of the guinea pigs and increases the action of adrenaline. It suppresses the heart of the rabbit and the uterus flatness. An intravenous injection into an anesthetized dog significantly lowers blood pressure, but it is not clear when injected into muscles and boats. In motion, it helps the lungs and the gods, stopping the blood and removing the phlegm. When the body weakened by the disease, cough, blood, pulmonary tuberculosis, dahan, anemia, ambulation, oil well, back pain is used.

The plum (Prunus mume) is named after the dried fruit from the pre-dried fruit. Lycoric acid, malic acid, wine acid, succinic acid, sitosterol, oleic acid, cetyl alcohol, and amidgalin are in the fruit. Fruit is used as raw material of lemon acid. Oats are used for diarrhea, fever, cough medicine, phlegm, stomach ache, vomiting and diarrhea.

Paeonia suffruticosa is a shrub with peonies and is planted with flowers and herbs from various places. It is used as a self-reliance medication for autonomic nervous system. It is used for fever, pain, and anti-inflammatory. It is used for menstrual irregularities, back pain, arthralgia. If you use peach seed (doen) together, the effect of anti-inflammation and blood purification is improved.

Inula Helenium is a perennial plant of Asteraceae with a rust of 50-150 cm. It is used for various respiratory diseases such as phlegm, placebo, diuretic, foot herb, bronchitis, pulmonary tuberculosis. It can also be used for vomiting, diarrhea, abdominal pain, stomach QA, lack of stomach and cold.

Pinellia ternate is a perennial herbaceous bulb which is 15-30 cm long from the genus. Let's face it because the leaves are in early summer. In the past, vomiting was used as a medicine to stop vomiting. Vomiting, vomiting, swallowing, and vomiting, vomiting, vomiting, coughing, dizziness, headache, acute gastritis, sore throat. In order to inhibit gastric hyperplasia, it is used together with dandelion.

Poria cocos are sclerotia which grows like a lump in parasitic on the pine root three to four years after cutting. It is usually 25 to 50 cm underground. It is a lump of about 30 ~ 50 ㎝ in diameter. It is called Baekbokryeong that the inside is white, and Daekryeong which the inside is pinkish. It has been used to remove swelling through diuretic action, to restore fatigue, and to remove water from the stomach. It has an effect on gastric juice and improves taste. Muscle spasms and dizziness are said to be effective.

The Rubus chingii is notorious as a raspberry. It protects the New Testament, helps the woman conceive, darkens the hair and brightens the eyes. It is used for the main purpose, and the barrel (strawberry strawberry) is also effective (the main barrel).

Asarum Sieboldii is a perennial herb with a rat. It is used for cold headache, fever, and sweating. It also goes into making silver.

The horse riding (Cimicifuga dahurica) is a perennial plant of the buttercup. The pharmacological effect of this plant is called ascending, so it looks like reverse osmosis and is called horseback riding. It is an early treatment for hypertension. It is used for headache, sleep disorder, dizziness, and heart disease. It has no effect on stage 3 of hypertension. For example, antipyretic, anti-inflammatory, analgesic, anti-inflammatory, and detoxification have been used as a cold medicine. It is also used for tonsillitis and stomatitis. The root of the plant, C. racemosa, is used in Europe as an analgesic for neuralgia and rheumatism.

Magnolia liliflora is an essential oil (Citral, Oegenol, Chineol, Caviacol), Pinene, Capronic acid and the like in the bud. Shin is used for headache, nasal congestion, sinusitis, rhinitis, dizziness and cold. It is also good for refreshing and improving memory.

The Artemisia vularis is a perennial herbaceous plant of the Asteraceae having a height of 0.8-1 m. It is used when there is a convergent hemostatic drug, anal bleeding, uterine bleeding, abdominal pain, blood stain, and also used as a tonic blood medicine. Rinse dry leaves to collect the hair on the surface and use it to moxibustion. Moxa should be collected in early June. Wormwood is used for uterine fibroids when unstable menstruation, abortion, pregnancy addiction. Fresh leaves can be bugged and attached to bites or snake bites.

The Achyranthes bidentata is called a steel knee by being like a cattle knee every stem. The dried root is called dried persimmon, and it writes to neuralgia, care, and women's disease.

Curcuma longa is a perennial herb that grows in the tropics. Its rootstock is cone-shaped or rounded columnar, and its surface is navy blue. In Oriental medicine, Oriental medicine is used as a placebo or bronchial medicine, and it is used for nosebleeds, hematemesis and hematuria. It is used for directional dry place, diuretic, antipyretic, extinguishing agent, acute chronic cholecystitis, cholangitis, quartz jaundice, gallstone disease and acute hepatitis.

The ginseng (Panax gienseng) is a perennial herb that belongs to the Acanthopanax senticosus. Ginseng has long been used as a preservative. Less than 2 to 10 g, more than 15 to 35 g once a month to eat. If you use ginseng as a first aid, you should eat 12 ~ 35 g of ginseng in water, or inject 2 ~ 4 cc of ginseng injections (including 0.57 g of ginseng in 1 cc of water) into muscle or vein to wake up . Especially, it is used as a first-aid medicine in combination with ginseng and rich man. Ginseng is used for cardiovascular diseases to repair hypotension or hypertension, and also for heart disease, heart atherosclerosis and angina pectoris. A small amount increases blood pressure and a large amount decreases blood pressure. Ginseng has a considerable effect on diabetes. It is also effective in nervous breakdown, psychosis, etc. It is effective for lethargy, stomach and gastrointestinal system weakness, and it is effective for symptom weakness such as vomiting.

The rootstock of the yam is Dioscorea japonica, also known as Sai. It is a good medicine, but it is also very good as a supplement. It has been used as a remedy for diseases such as recovery from illness, general weakness, weakness and fatigue, and has been used especially for the sick and the elderly. It is a nutritional tonic containing many nutrients. In the private sector, it is used for enuresis, oil wells, and cold sweat.

The outpost (Rubia akane) brightens the eyes, eliminates fraud and debauchery, and keeps cold temperatures from becoming less severe. In the fruit skin, essential oil (main component Dipentene-diel-limonene, citrolelal, etc.), the acid component compound of taste ingredient, hiperine, unsaturated fatty acid amide and the like are cited. Fruit contains xanthoxine which is convulsive poison, xanthoxine acid which is paralyzed ingredient, And campesterol. Spicy taste of fruit has local paralysis. Sanzole has an antiparasitic activity, and it has an effect against various pathogenic bacteria. Anti-inflammatory, anti-bacterial, analgesic action can be used for gastroenteritis, gastric enlargement, etc. It is also used for neuralgia and rheumatism as a bath.

The Trichosantes Kirilowii is a perennial herbaceous plant of Chakutari. The roots are called Chunhwabun, Chungryeong, and in the fall, the Casser is peeled off and dried in the sun. Root powder is called as a flower because it is white like snow. Consent is mainly used when there is a lack of sensory and sensory stimulation. It is also used to treat jaundice using anti-inflammatory action. The roots are used for the treatment of fever caused by fever, diarrhea, diarrhea, jaundice, pulmonary hemorrhage, hemorrhoids, and fever due to the effects of life, Fruit is used for inflammation, cold water, cold sores, cough and constipation, asthma, angina pectoris. It is used to treat seawater, chest, dysentery, dyspepsia, dyspepsia, jaundice, constipation, and early stomach because of the efficacy of decongestion, laxity, The fruit husk is used to treat dysentery, human pain, chest pain, hematemesis, non-hemorrhage, diabetes, constipation, and poisonous poison due to the efficacy of paroxetine, Seeds are used to treat mild seawater, constipation constipation, herbaceous mites, and locusts due to the efficacy of decongestion, laxity, and ephedra.

The gardenia (jasminoides) is the fruit of evergreen shrub with madder. Gardenia is also often used as a food or fabric paint. Inflammation, hemorrhage, hemorrhage, myocarditis, hematemesis, hemorrhage, jaundice. In the private sector, flour and white powder are blended with flour and vinegar to bruise and torn wounds.

The Polygonum multiflorum is a perennial herb of broad-leaved spruce. In Han, it has been used for a long time since it has been suffering from sickness, nervous breakdown, and scholastic achievement. In particular, it is said to be effective in enhancing male sexual performance.

The golden (Scutellaria baicalensis) is a perennial herb of Lamiaceae and reaches about 1 m. The roots are yellow and called yellow. Total pravnoids are separated and used for acute and chronic hepatitis and cholecystitis. Root alcohol extracts are hypotensive agents, sedatives, hypertension, cardiovascular disorders, dysfunction of the nervous system, sleep disturbances and palpitations. It is used for removing inflammation such as lungs and large intestines with inflammatory drugs and antipyretic drugs.

The ginseng caterpillar fungus can be searched for the efficacy of ginseng and the efficacy of caterpillar fungus at the same time.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, it is possible to use the liquorice marketed as a herb medicine, such as licorice, cinnamon, gobon, gosam, gugija, mung bean, jujube, fungus, codworm, plum, , Ginseng, yams, chrysanthemum, chrysanthemum, gardenia, garlic, golden or ginseng caterpillar fungus is finely pulverized and added to water, such as 5 to 20 parts by volume of water, or anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms The extracts of the medicinal materials are extracted by heating at 50 to 100 ° C for 1 to 5 hours in an extractor equipped with a reflux condenser as an organic solvent. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be a medicinal mushroom mold such as Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. have.

More specifically, when yeast is used as a microorganism, the concentration of the yeast (about 10 ⁵ to 10 ⁷ cells / mL) is lower than that of licorice, cinnamon, gobon, gosam, gugija, mung bean, (Primary culture medium containing YM medium (primary culture medium), primary culture medium (primary culture medium), primary culture medium, primary culture medium, and primary extracts of Chinese cabbage, ginseng, ginseng, ), And then cultured for 30 to 120 hours while maintaining the conditions of pH 5.5 to 6.5, temperature 25 to 35 DEG C, aeration amount 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactobacillus is used as a microorganism, there is a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) of licorice, cinnamon, gobon, gosam, gugija, mung bean, jujube, cheonghwamun, (MRS medium) containing the first extracts of Bokbunja, Sechin, Horse riding, Shin, Wormwood, Woosu, Ulgum, Ginseng, Yams, Chunghwa, After that, the cells are cultured for 24 to 120 hours while maintaining the pH of 6.5 to 7.5, the temperature of 35 to 38 DEG C, and the aeration amount of 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When bifidobacteria are used as microorganisms, they are used at a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) in the order of licorice, cinnamon, gobon, gosam, gugija, mung bean, jujube, (BL medium) containing primary extracts of Baekbokwolryeong, Bokbunjae, Seisin, Horse riding, Shinyi, Wormwood, Ulysseed, Woogeum, Ginseng, Yams, Chuncheon, Chrysanthemum, Gardenia, After inoculation, the cells are cultured for 24 to 120 hours at pH 6.5 to 7.5 and at a temperature of 35 to 38 캜, while maintaining anaerobic culture conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

In addition, when fungi is used as a microorganism, it is possible to use fungi such as licorice, cinnamon, gobon, ginseng, gugija, mung bean, jujube, Fungus is inoculated at 1 to 10% by weight in each of corn, worm, wool, ginseng, yam, chestnut, chrysanthemum, gardenia, Solid fermentation at 60 to 70% for 60 days to obtain each of the medicinal fermented extracts.

When the free radical scavenging activity of DPPH is measured, the fermented extracts of Korean traditional medicines exhibit a remarkably superior antioxidative effect as compared with general herbal extracts. In addition, when the cosmetic composition is prepared by using the above fermented extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin, exhibits skin aging and antioxidant effect.

The obtained medicinal fermented extract may be contained in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight, in the cosmetic composition having antioxidative activity of the present invention. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition having the antioxidative activity containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the medicinal fermented extract of the present invention. In addition to the fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition having antioxidative activity of the present invention may contain, in addition to the above-described ingredients, the components described above, such as oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, dye, preservative, And the like can be appropriately used in combination as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, mung bean, jujube, 100 g of each ground product obtained by grinding and milling Chunghwa powder, gardenia, ginseng, golden and ginseng caterpillar fungus were added to 3 L of purified water or 80% methanol, and the mixture was boiled for 3 hours in a reflux extractor equipped with a cooling condenser. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of medicament according to the extraction solvent.

Medicinal 80% methanol Purified water licorice 8.3 g 7.9 g cinnamon 7.9 g 7.6 g Gobon 8.5 g 8.1 g GoSam 8.5 g 8.2 g Gugija 9.7 g 9.1 g green gram 6.8 g 6.5 g Jujube 10.2 g 9.3 g Solo 8.8 g 8.1 g Cordyceps 6.6 g 6.1 g plum 6.5 g 6.0 g Mural 9.1 g 8.5 g elecampane 9.5 g 9.2 g Inferiority 11.2 g 9.8 g Wind wind 7.4 g 6.7 g Baekbokryung 8.5 g 7.8 g Bokbunja 10.7 g 10.1 g Seisin 6.5 g 5.9 g riding 7.5 g 6.8 g God 6.3 g 6.0 g Wormwood 8.1 g 7.5 g The 6.2 g 5.5 g Kool 9.1 g 8.2 g Ginseng 6.4 g 6.0 g yam 10.8 g 10.2. g Thousand 7.2 g 6.7 g Light flux 7.3 g 6.8 g Gardener 5.9 g 5.0 g Sewage 8.6 g 8.1 g Gold 6.8 g 6.1 g Ginseng caterpillar 6.5 g 6.1 g

Examples 2 to 31

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 2 (licorice) 5.71 Example 17 (Rubella) 5.98 Example 3 (cinnamon) 6.13 Example 18 (Sezin) 5.89 Example 4 (Giant) 6.11 Example 19 (Horse riding) 5.83 Example 5 (Gosam) 5.96 Example 20 (Shin) 6.06 Example 6 (Kouji) 6.05 Example 21 (Writhing) 6.12 Example 7 (Green bean) 5.89 Example 22 (mist) 5.87 Example 8 (jujube) 6.11 Example 23 (Ulm) 5.89 Example 9 (Solubility) 5.87 Example 24 (Ginseng) 6.15 Example 10 (Cordyceps) 6.16 Example 25 (Yam) 6.07 Example 11 (plum) 6.02 Example 26 (thousand seconds) 5.88 Example 12 (Mulch) 6.18 Example 27 (Chrysanthemum) 6.22 Example 13 (vinegar) 6.18 Example 28 (Gardenia) 5.91 Example 14 (inferior) 6.12 Example 29 (HSAO) 6.11 Example 15 (windshield) 5.87 Example 30 (golden) 6.12 Example 16 (White rosewood) 6.10 Example 31 (ginseng caterpillar fungus) 6.25

Examples 32 to 61

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 32 (licorice) 5.73 Example 47 (Rubella) 6.13 Example 33 (cinnamon) 6.10 Example 48 (Sezin) 5.97 Example 34 (Giant) 6.02 Example 49 (Horse riding) 6.12 Example 35 (Gosam) 5.88 Example 50 (Shin) 5.97 Example 36 (Kugija) 6.28 Example 51 (wormwood) 6.02 Example 37 (Green bean) 5.91 Example 52 (washed) 5.94 Example 38 (jujube) 6.05 Example 53 (Ulm) 6.18 Example 39 (Solubility) 6.19 Example 54 (Ginseng) 6.09 Example 40 (Cordyceps) 6.11 Example 55 (Yam) 612 Example 41 (plum) 5.97 Example 56 (thousandths of a second) 5.99 Example 42 (Mulch) 6.13 Example 57 (Chrysanthemum) 6.09 Example 43 (vinegar) 5.92 Example 58 (Gardenia) 5.95 Example 44 (inferior) 5.96 Example 59 (HSAO) 6.01 Example 45 (windshield) 5.95 Example 60 (golden) 5.96 Example 46 (White rosewood) 5.99 Example 61 (ginseng caterpillar fungus) 6.19

Examples 62 to 91

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 62 (licorice) 5.83 Example 77 (Bokbunja) 5.96 Example 63 (cinnamon) 6.08 Example 78 (Sezin) 6.18 Example 64 (Giant) 6.13 Example 79 (Horse riding) 6.14 Example 65 (Gosam) 6.01 Example 80 (Shin) 6.12 Example 66 (Kouji) 6.17 Example 81 (wormwood) 5.98 Example 67 (Green bean) 6.12 Example 82 (squal) 6.07 Example 68 (jujube) 6.22 Example 83 (Ulm) 6.07 Example 69 (Solubility) 5.93 Example 84 (Ginseng) 6.15 Example 70 (Cordyceps) 6.13 Example 85 (Yam) 6.10 Example 71 (plum) 6.09 Example 86 (thousand seconds) 6.11 Example 72 (Mulch) 6.02 Example 87 (Chrysanthemum) 6.26 Example 73 (vinegar) 6.02 Example 88 (Gardenia) 6.11 Example 74 (opposite) 6.21 Example 89 (HSAO) 5.89 Example 75 (windshield) 6.11 Example 90 (golden) 6.15 Example 76 (Baesungwolryeong) 5.91 Example 91 (Ginseng Cordyceps) 6.21

Examples 92 to 119

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight of fermentation broth (g) Medicinal Dry weight of fermentation broth (g) Example 92 (licorice) 7.52 Example 106 (Rubella) 9.37 Example 93 (cinnamon) 7.15 Example 107 (Sezin) 5.75 Example 94 (Giant) 7.83 Example 108 (Horse riding) 6.71 Example 95 (Gossam) 7.74 Example 109 (Shin) 5.62 Example 96 (Gugija) 8.79 Example 110 (Wormwood) 7.12 Example 97 (Green bean) 6.12 Example 111 (wax) 5.43 Example 98 (jujube) 8.86 Example 112 (Ulm) 8.05 Example 99 (Solubility) 7.91 Example 113 (ginseng) 5.43 Example 100 (plum) 5.77 Example 114 (Yam) 9.36 Example 101 (Mulch) 8.08 Example 115 (thousandths of a second) 6.15 Example 102 (vinegar) 8.51 Example 116 (Chrysanthemum) 6.23 Example 103 (below) 9.91 Example 117 (Gardenia) 5.38 Example 104 (windshield) 6.62 Example 118 (Hassuo) 7.72 Example 105 (White rosewood) 7.11 Example 119 (golden) 6.07

Experimental Example 1. Free radical scavenging rate

The free radical scavenging activity was measured to confirm the antioxidative activity of each of the medicinal substance itself and the fermented extract of the medicinal materials of Examples 2 to 119 described above.

Each of the medicinal materials themselves and the medicinal fermentation extracts of Examples 2 to 119 were used as test samples, and the free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co. (Sigma Co., Ltd, USA) and used. First, a standard DPPH solution with a concentration of 400 mu m was prepared. Then, ethanol was added to each of the medicinal materials themselves, ascorbic acid as an antioxidant as a fermentation extract of the medicinal fermentation extracts of Examples 2 to 119 and a reference material, respectively, to prepare a sample at a concentration of 200 μg / ml. Then, the sample and standard DPPH solution were added at the same ratio, stirred well, reacted at 37 ° C for 30 minutes, and absorbance was measured at 520 nm. At this time, ethanol was added instead of the fermented extract of the medicinal product to prepare a control. The free radical scavenging ratio (%) was determined using the following equation (1), and the results are shown in Tables 6 to 10 below.

[Equation 1]

sample
(Final concentrations are all 0.005%, except for the control group) Free radical scavenging rate (%) Control group (no addition) 0 YM badge 9.5 MRS badge 10.3 BL medium 11.1 Ascorbic acid 42.7 licorice Medicinal self 30.8 The yeast fermented extract of Example 2 57.5 The fermented extract of lactic acid bacteria of Example 32 54.2 The bifidobacterial fermentation extract of Example 62 47.9 The fungal fermentation extract of Example 92 45.1 cinnamon Medicinal self 35.2 The fermented yeast extract of Example 3 60.1 The fermented extract of lactic acid bacteria of Example 33 57.9 The bifidobacterial fermentation extract of Example 63 57.1 The fungal fermentation extract of Example 93 51.4

sample Free radical scavenging rate (%) Gobon Medicinal self 31.2 The fermented yeast extract of Example 4 45.2 The fermented extract of lactic acid bacteria of Example 34 42.9 The bifidobacterial fermentation extract of Example 64 40.2 The fungal fermentation extract of Example 94 43.2 GoSam Medicinal self 33.8 The fermented yeast extract of Example 5 57.3 The fermented extract of lactic acid bacteria of Example 35 55.1 The bifidobacterial fermentation extract of Example 65 49.3 The fungal fermentation extract of Example 95 47.2 Gugija Medicinal self 38.9 The yeast fermented extract of Example 6 62.1 The fermented extract of lactic acid bacteria of Example 36 30.8 The bifidobacterial fermentation extract of Example 66 55.5 The fungal fermentation extract of Example 96 51.9 green gram Medicinal self 35.7 The yeast fermented extract of Example 7 51.2 The fermented extract of lactic acid bacteria of Example 37 50.3 The bifidobacterial fermentation extract of Example 67 52.2 The fungal fermentation extract of Example 97 45.9 Jujube Medicinal self 31.5 The yeast fermented extract of Example 8 45.9 The fermented extract of lactic acid bacteria of Example 38 41.2 The bifidobacterial fermentation extract of Example 68 43.7 The fungal fermentation extract of Example 98 39.8 Solo Medicinal self 33.9 The yeast fermented extract of Example 9 55.7 The fermented extract of lactic acid bacteria of Example 39 54.2 The bifidobacterial fermentation extract of Example 69 50.1 The fungal fermentation extract of Example 99 47.3 Cordyceps Medicinal self 38.9 The yeast fermented extract of Example 10 62.7 The fermented extract of lactic acid bacteria of Example 40 60.7 The bifidobacterial fermentation extract of Example 70 59.6 plum Medicinal self 36.1 The fermented yeast extract of Example 11 55.4 The fermented extract of lactic acid bacteria of Example 41 56.3 The bifidobacterial fermentation extract of Example 71 50.6 The fungal fermentation extract of Example 100 47.7 Mural Medicinal self 31.1 The fermented yeast extract of Example 12 42.1 The fermented extract of lactic acid bacteria of Example 42 40.6 The bifidobacterial fermentation extract of Example 72 39.5 The fungal fermentation extract of Example 101 41.1

sample Free radical scavenging rate (%) elecampane Medicinal self 34.8 The fermented yeast extract of Example 13 59.5 The fermented extract of lactic acid bacteria of Example 43 55.4 The bifidobacterial fermentation extract of Example 73 49.6 The fungal fermentation extract of Example 102 48.1 Inferiority Medicinal self 31.9 The fermented yeast extract of Example 14 55.3 The lactic acid bacteria fermented extract of Example 44 57.3 The bifidobacterial fermentation extract of Example 74 51.1 The fungal fermentation extract of Example 103 52.7 Wind wind Medicinal self 33.4 The yeast fermented extract of Example 15 59.5 The fermented extract of lactic acid bacteria of Example 45 55.2 The bifidobacterial fermentation extract of Example 75 47.5 The fungal fermentation extract of Example 104 46.6 Baekbokryung Medicinal self 30.5 The yeast fermentation extract of Example 16 50.2 The fermented extract of lactic acid bacteria of Example 46 49.2 The bifidobacterial fermentation extract of Example 76 51.9 The fungal fermentation extract of Example 105 47.3 Bokbunja Medicinal self 36.9 The yeast fermented extract of Example 17 65.1 The fermented extract of lactic acid bacteria of Example 47 61.1 The bifidobacterial fermentation extract of Example 77 55.8 The fungal fermentation extract of Example 106 57.4 Seisin Medicinal self 35.5 The yeast fermented extract of Example 18 47.5 The fermented extract of lactic acid bacteria of Example 48 47.3 The bifidobacterial fermentation extract of Example 78 42.1 The fungal fermentation extract of Example 107 43.1 riding Medicinal self 32.1 The yeast fermentation extract of Example 19 43.2 The fermented extract of lactic acid bacteria of Example 49 42.2 The bifidobacterial fermentation extract of Example 79 40.9 The fungal fermentation extract of Example 108 41.1 God Medicinal self 33.8 The yeast fermented extract of Example 20 55.2 The fermented extract of lactic acid bacteria of Example 50 50.9 The bifidobacterial fermentation extract of Example 80 47.1 The fungal fermentation extract of Example 109 49.9 Wormwood Medicinal self 38.9 The fermented yeast extract of Example 21 58.3 The fermented extract of lactic acid bacteria of Example 51 55.1 The bifidobacterial fermentation extract of Example 81 57.6 The fungal fermentation extract of Example 110 55.5

sample Free radical scavenging rate (%) Ginseng Medicinal self 38.9 The yeast fermented extract of Example 24 70.2 The fermented extract of lactic acid bacteria of Example 54 67.5 The bifidobacterial fermentation extract of Example 84 72.1 The fungal fermentation extract of Example 113 65.5 The Medicinal self 31.2 The yeast fermented extract of Example 22 47.6 The fermented extract of lactic acid bacteria of Example 52 45.9 The bifidobacterial fermentation extract of Example 82 41.3 The fungal fermentation extract of Example 111 39.4 Kool Medicinal self 33.8 The fermented yeast extract of Example 23 51.3 The fermented extract of lactic acid bacteria of Example 53 53.2 The bifidobacterial fermentation extract of Example 83 50.1 The fungal extract of Example 112 55.9 yam Medicinal self 35.4 The fermented yeast extract of Example 25 59.4 The fermented extract of lactic acid bacteria of Example 55 55.6 The bifidobacterial fermentation extract of Example 85 50.3 The fungal fermentation extract of Example 114 52.6 Thousand Medicinal self 31.2 The yeast fermented extract of Example 26 49.5 The fermented extract of lactic acid bacteria of Example 56 45.2 The bifidobacterial fermentation extract of Example 86 40.6 The fungal fermentation extract of Example 115 43.2 Light flux Medicinal self 37.3 The yeast fermented extract of Example 27 60.4 The fermented extract of lactic acid bacteria of Example 57 57.3 The bifidobacterial fermentation extract of Example 87 55.1 The fungal fermentation extract of Example 116 56.7 Gardener Medicinal self 35.1 The fermented yeast extract of Example 28 51.1 The fermented extract of lactic acid bacteria of Example 58 45.3 The bifidobacterial fermentation extract of Example 88 47.6 The fungal extract of Example 117 47.1 Sewage Medicinal self 36.6 The yeast fermented extract of Example 29 55.2 The fermented extract of lactic acid bacteria of Example 59 53.8 The bifidobacterial fermentation extract of Example 89 48.6 The fungal fermentation extract of Example 118 45.3 Gold Medicinal self 37.8 The fermented yeast extract of Example 30 61.2 The fermented extract of lactic acid bacteria of Example 60 60.3 The bifidobacterial fermentation extract of Example 90 57.8 The fungal fermentation extract of Example 119 59.5

sample Free radical scavenging rate (%) Ginseng caterpillar Medicinal self 38.5 The fermented yeast extract of Example 31 68.2 The fermented extract of lactic acid bacteria of Example 61 72.4 The bifidobacterial fermentation extract of Example 91 68.9

As shown in Tables 6 to 10, each of the fermentation extracts obtained according to Examples 2 to 119 had a free radical scavenging rate as compared with the respective medicinal materials themselves or the basic medium or ascorbic acid used as a known antioxidant All were high.

Example  120 and Comparative Example  One

Using the fermentation extract of licorice fungus of Example 92, ointment for external skin was prepared according to the prescription of Table 11 below.

division Example 120 Comparative Example 1 Licorice fungus fermentation extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 121 and Comparative Example 2

Using the fermented licorice fermented extract of Example 92, a cream was prepared according to the prescription of Table 12 below.

division Example 121 Comparative Example 2 Licorice fungus fermentation extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 122 and Comparative Example 3

Using the fermentation extract of licorice fungus of Example 92, a softening longevity was prepared according to the formulation shown in Table 13 below.

division Example 122 Comparative Example 3 Licorice fungus fermentation extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 123 and Comparative Example 4

Using the fermentation extract of licorice fungus of Example 92, a nutritional lotion was prepared according to the formulation shown in Table 14 below.

division Example 123 Comparative Example 4 Licorice fungus fermentation extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 124 and Comparative Example 5

Using the fermentation fungus fermentation extract of Example 92, a pack was prepared according to the prescription of Table 15 below.

division Example 124 Comparative Example 5 Licorice fungus fermentation extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 125 and Comparative Example 6

Using the fermented licorice fermented extract of Example 92, an essence was prepared according to the prescription of Table 16 below.

division Example 125 Comparative Example 6 Licorice fungus fermentation extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example 2. Anti-Aging Effect

The anti-aging effect of the cosmetic composition containing the licorice fermented extract was verified as follows.

Twenty hairless mice of 15 to 20 weeks of age were used per experiment group as follows. Ultraviolet light was irradiated at 2 mJ / cm 2 dose using an ultraviolet light irradiator (UV simulator, US) on the dorsal area of hairless mice, and this was induced for 2 weeks per week for 4 weeks to induce photoaging of the skin of mice. Thereafter, the cosmetic composition containing the licorice fermented extract of Examples 120 to 125 was applied to one side of the hairless mouse at an appropriate amount twice a day for two weeks, and on the other side, licorice fermented extract The cosmetic compositions of Comparative Examples 1 to 6, which were not contained, were applied. At this time, the packs of Example 124 and Comparative Example 5 were peeled off after 15 minutes. After 2 weeks, the effect of aging was compared with the naked eye, and the results were evaluated in three stages of distinct effect, effect, and no difference. The results are shown in Table 17.

division Distinct effect (n.) Effective (persons) No difference (persons) Example 120 6 8 6 Example 121 3 9 8 Example 122 3 10 7 Example 123 4 7 9 Example 124 3 8 9 Example 125 2 9 9

As shown in Table 17, the cosmetic compositions containing the licorice fermented extract prepared according to Examples 120 to 125 exhibited an anti-aging effect against at least 10 of 20 subjects, and in particular, a large amount of licorice fermented extract In Comparative Example 1 in which the extract of Example 120 was applied and Comparative Example 1 in which the extract of licorice fermentation was not applied showed an anti-aging effect of 70% and no side effects were observed in the skin. Thus, the licorice fermented extract was safe and highly effective It was found that it was an anti-aging cosmetic product.

Claims (4)

Which comprises an extract of fermented mildew of mushroom as an active ingredient,
The fermented extract may contain at least one fungi selected from the group consisting of Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica, phellinus linteus and Ganoderma lucidum, And a fermentation extract obtained by solid fermentation.
delete delete The method according to claim 1,
Wherein the fermented extract is contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.