KR101457960B1 - Cosmetic composition for preventing of kin trouble - Google Patents

Abstract

The present invention relates to a cosmetic composition for relieving skin troubles, and more particularly, to a cosmetic composition for relieving skin troubles, which comprises a fermented extract of a medicinal substance selected from at least one group selected from the group consisting of Gakyung, Maechian, Maekmundong, Bogolji, The present invention relates to a cosmetic composition for relieving skin troubles. The cosmetic composition for relieving skin troubles according to the present invention is safe for the skin because of its excellent anti-inflammatory effect, which is a skin tear-reducing effect.

Cosmetics, fermentation extract, anti-inflammation, skin trouble

Description

[0001] The present invention relates to a cosmetic composition for preventing skin trouble,

The present invention relates to a cosmetic composition for relieving skin troubles, and more particularly, to a cosmetic composition for relieving skin troubles which is excellent in anti-inflammatory effect, which is a skin troubles reducing effect, and is safe for skin.

Cosmetics are products used to protect the skin and beautify and clean the skin, but the composition thereof inevitably includes ingredients that are different from the purpose of skin protection. These ingredients include surfactants, preservatives, fragrances, sunscreens, pigments, as well as various ingredients for imparting other efficacy and effects. These ingredients are generally known to cause skin irritation, rashes, swelling and other problems (Maibach, H., I. Contact Dermatitis, 6. 369-404, 1980). In addition, sebum and sweat discharged from the body, fatty acids in cosmetic ingredients, higher alcohols, protein components and the like may be decomposed into substances having high toxicity by the skin-on-ground bacteria existing on the skin, It is well known that skin inflammation is caused by ultraviolet rays from the sun.

Inflammation reactions are manifested in five phenomena, such as feeling of redness, pricking feeling, burning hotness, swelling, and tissue changes, which may be caused by harmful environmental conditions, It is a physiological reaction to protect a living body from intrusion and mechanical damage. These inflammatory phenomena lead to many increases of various types of leukocyte (PMNs) and immune substances, and these increased cells secrete various kinds of inflammatory cell products, such as proteolytic enzymes and cytokines, I can do it. However, this action may cause harmful damage to adjacent tissue cells and non-cellular components. Therefore, under proper conditions, the normal function is restored after the initial state of inflammation. However, if irritant stimulating agents are not eliminated or continuously produced, chronic inflammation occurs as a result, resulting in more serious tissue damage.

Atopy and acne, forms of skin troubles related to inflammation, are described as follows.

Atopy is characterized by severe itching. When it irritates the skin, the itching becomes worse, and when it is scratched more and more, it becomes scratched, and the bacterial infection (Staphylococcus aureus, Streptococcus etc.) occurs and inflammation occurs. One of the important factors in the problem of atopy is the secondary infections and superantigen toxins that occur at this time. 80-90% of atopic patients are infected with Staphylococcus aureus and Streptococcus pyogenes in their skin, where Anit-Staphylococcus IgE is produced, which causes the superantigen toxin It is also triggered by and aggravates. The superantigen toxin of Staphylococcus aureus selectively stimulates T lymphocytes through antigen presenting cells in Langerhans cells to produce interleukin-1 and TNF-alpha, and T-cell stimulated by these cells produces various intracellular cytokines And it causes inflammation. In other words, the superantigen toxin promotes the secretion of interleukin-4 and interleukin-5, thereby inhibiting the secretion of IFN-r and increasing the number of CLA, thereby promoting the production of IgE.

Second, patients infected with Staphylococcus aureus secrete histamine from the basophil by the IgE antibody to the staphylococcus toxin, which leads to worsening of atopic dermatitis.

Therefore, the use of moisturizers to prevent skin dryness is frequently recommended, and secondary infection and its control of superantigen factors are very important. In other words, atopy appears to be a basic pathogenesis of genetic defects, but it is triggered by various external factors.

The causes of atopic eczema include genetic factors, sensitive and dry skin, metabolic factors, excessive production of IgE, abnormalities of the autonomic nervous system, psychological factors, and irritating factors.

Atopy is a disease whose mechanism is not simple. From an immunological viewpoint, it is not an immunological problem but a problem of various patterns. For example, the problem of gamma interferon, which inhibits excessive production of IgE, may occur due to the fact that its production is lower than the average value, but it is smaller than interleukin-4, which has opposite action, And even if it is normal in terms of quantity or quality, sensitivity to the stimulation of interferon may be reduced.

On the other hand, acne can be divided into inflammatory acne lesions and non-inflammatory (acne vulgaris) acne lesions depending on symptoms.

Inflammatory acne lesions cause sebaceous matter to escape from cottonseed and cause inflammation reaction to surrounding skin tissue, which is red due to inflammation. Squeezing or pressing the acne buds will cause the cotton buds to burst into inflammatory acne lesions, which increases the risk of leaving acne scars.

Basically, there are three types of inflammatory acne lesions: papules, pustules, and nodules.

The acne acne (red acne) is as follows. The term "papule" refers to a small, firm red lesion that sometimes appears as an intermediate form of non-inflammatory acne lesions and acne lesions, and is accompanied by mild inflammation, so the hue appears red, . So, the acne that has sprung out is called acne acne. This is an inflammatory acne, as described above, which varies in size, hardness, and degree of lightness depending on the degree of inflammation. The more inflammation, the bigger, the more vivid and the longer it tends to go. Less irritated ones are just a few (about 7%) just fading, but most of the time they become more inflamed or become acne acne.

The pustular acne (acne acne) is as follows. It is like a battlefield after a fierce battle. In other words, there are some bacteria, dead tissue, and white blood cells that collect bacteria to get rid of the bacteria. Pustular pustules are small and round in shape, similar to papules, but they are different from papules in that they contain paprika. There is no bacteria in bacteria such as bacteria, and it is the result of inflammation reaction by chemical stimulation by ingredients such as free fatty acid in sebum. Among the acne acne, small, harder ones around are faster than the larger, harder ones.

Next, nodular acne is the most severe form of acne and is large in size, inflammatory, and contains a large amount of agar. The nodules form the nectar by inducing the immune response to the skin tissue around the nectar from the contents of the cotton. Nodular acne is relatively deep in the skin and can be accompanied by pain, and is more likely to leave an acne scar during the healing process than other acne lesions.

The core substance of these various inflammatory processes is arachidonic acid, which is converted from cell membrane lipids by an enzyme called stimuli-activated phospholipase A2 (PLA2), and arachidonic acid is converted to cyclooxygenase (Prostaglandins), leukotrienes (Leukotrienes) via mediators of inflammation (Thomas, R., et al., &Quot; COX ") or lipoxygenase Rev. Physiol. Biochem. Pharmacol., 100, 1984).

Recently, researches on methods to reduce skin irritation and inflammation have been actively carried out. As a result of the researches so far, neural peptides such as substance P (Substance P) Kinases such as bradykinin activated by plasma, or various cytokines, prostaglandin E2 (hereinafter abbreviated as "PGE2") formed by the COX pathway, are involved in edema formation and vasodilation, Therefore, neuropeptide antagonists such as substance P, a bradykinin antagonist and a COX inhibitor have been proposed as anti-inflammatory agents, but the known anti-inflammatory agents have been proposed to be included in the product in terms of safety against skin And its use is limited because it has most problems in terms of stability.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

Accordingly, it is an object of the present invention to provide a cosmetic composition for relieving skin troubles which is excellent in anti-inflammatory effect, which is a skin troubles reducing effect, and is safe for skin.

Another object of the present invention is to provide a cosmetic composition for alleviating skin troubles which further enhances the anti-inflammatory effect by further strengthening the pharmacological effect of each medicinal substance through fermentation.

In order to achieve the above object, the present invention provides a fermented extract of a medicinal substance selected from the group consisting of Gakyung, Maji-hyeon, Maekmundong, Bogolji, Seijiji, Yeonchunho, The present invention provides a composition for relieving skin troubles.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

Hereinafter, the cosmetic composition for skin troubles of the present invention will be described in detail.

The medicinal plant extracts described in the present invention can be obtained by extracting a medicinal substance selected from at least one kind selected from the group consisting of Gakyung, Mahi, Maekmundong, Bogolji, Seijiwang, Yeonchun, It means. Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The inventors of the present invention have conducted studies to develop fermented Chinese medicinal materials having excellent skin troubles and have found that a fermentation broth such as Gakyung, Machi Hyun, Maekmundong, Bogolji, Jijiji, Yeonchun, The present invention has been accomplished based on this finding.

The cosmetic composition for relieving skin troubles according to the present invention contains a fermented extract of a medicinal substance selected from the group consisting of Gakyung, Maji, Maekmundong, Bogolji, Sejangbu, Yeonchun, do.

Platicodon grandiflorum is a perennial herbaceous plant of the bellflower, and its root is washed and dried. Borage in bamboo is used as a phlegm drug for cough, bronchitis, pesticide, bronchitis, tonsillitis. The blooming and anti-inflammatory action of the bellflower is also applied to relieve the inflammation of the stomach.

The above-mentioned Portulaca oleracea has been used for the treatment of sores and boils since ancient times, and has a sterilizing and detoxifying action and a heat-releasing effect, and is widely used as a raw material for female drums. It protects the skin from the external environment with its skin protection function, and is effective in improving acne skin, sensitive skin, allergic skin, dry skin.

The Liriope platyphylla is a nutrient muscle of the mistletoe of the lily family, which is washed and washed with caesar in autumn. The root of this plant is similar to barley, and it does not dwell in winter. McMundong is used for fever reduction, anti-inflammatory drugs, coughing or phlegm withdrawal, sore throat, dry cough, persistent fever, constipation, diuretic. In addition, it is a medicine for tongue, and it is also good for angina because it has a strong effect.

Psoralea corylifolia is an annual herb with soybeans. It is said that the dried fruit of the fenugreek is dried or digged. It is effective against leukemia and alopecia areata. In Han, the fruit is used as a tonic to pass on the back, sexual dysfunction, diarrhea and pimples.

Rehmannia glutinosa is a perennial herbaceous herbaceous perennial plant with a height of 15 to 20 cm. It is washed in water in the autumn. The raw thing is called raw persimmon, and the dried persimmon is called persimmon. It is used for the protection of the kidney with blood, medicine, stomach, and diuretic, and is used for anemia, weakness, bleeding, hemorrhage and tuberculosis.

The above-mentioned Papaver rhoeas uses flower fruit, and it is effective as Jinhae, painkiller, and branch medicine.

Nelumbo nucifera can be used as a pain reliever by extracting Roemerin and Nuciferin from roots. The lotus (seed of the lotus) has a gentle action, has the effect of lowering fever and relieving thirst. And since the lecture reduces the semen, it is used in the wells and the wandering. Stretchers are also tonic to help digestion and strengthen stomach.

Ponciruc trifoliate is a deciduous shrub of more than an acid and has a lot of stalk on its stem. The fruit is a tangerine, and a young tangerine fruit is called a persimmon, while a grown fruit is called a crust. It is also known as the young fruit of the clam. It is used when the digestion is not done and the humidification and the stomach are sore. It is also used as expectorant and diuretic.

The Ligusticum officinale is a perennial plant of the Mineral. From time immemorial, it has been used with angiopathic medicines, blood red blood drugs, tranquilizers, sedatives, anemia, poor circulation, menstrual irregularities, and gynecological diseases. Not only root but also outposts have the same therapeutic effect. Especially, because of the painful effect, the headache must be inserted into the head palm. Chestnut, chrysanthemum, and headache are used for chestnut headache.

Asparagus lucidus is a perennial herbaceous plant of the genus Lilium, and its stem is 1 ~ 2 m long and it grows and has many branches with a line shape. Leaves are needle-like lanceolate. The fruit is round and whitish and grows in the coastal zone of central and southern China. The roots of the roots are called astronomical roots, and in the fall, the roots are peeled off and steamed. It is said that this plant is similar to Maeummundong because it is vine. In Han, it is used for nourishing tonic medicine, cough medicine for cough and sputum caused by vulgaris, and hardening of mutation.

Cuscuta japonica is an annual plant of a Japanese marsh. In one room, Gangjeong medicine is used as a medicine for vomiting, oil, and low back pain. It is also used as sedative, analgesic and laxative. In the private sector, the face is washed to eliminate papules (pustules) from the pancreatic juice.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, it is finely pulverized by purchasing and grinding 5 to 20 parts by volume of water, 1 to 4 parts by mass of carbon atoms per 1 part by dry weight of pulverized material, The extracts of the medicinal materials are extracted by heating in an extractor equipped with a reflux condenser with an organic solvent such as anhydrous or low-boiling alcohol at 50 to 100 ° C for 1 to 5 hours. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be a medicinal mushroom mold such as Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. have.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and has a specific concentration of about 10 ⁵ to 10 ⁷ cells / mL in the case of yeast, Yeast is inoculated in a primary culture medium (YM medium) containing the first extracts, and cultured for 30 to 120 hours under conditions of pH 5.5 to 6.5, temperature 25 to 35 ° C, aeration amount 0.05 to 0.5 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactic acid bacteria are used as microorganisms, the first extracts of Gakyung, Maji, Maekmundong, Bogoriji, Seijiwang, Yeonchun, Yeonwoo, Jisil, Taekgung, Chunmungdong and Tosan are contained at a constant concentration (about 10 ⁵ to 10 ⁷ cells / (MRS medium), and cultured for 24 to 120 hours while maintaining the conditions of pH 6.5 to 7.5, temperature 35 to 38 ° C, aeration amount 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When using the bifidobacteria microorganisms are respectively a constant concentration (about 10 ⁵ ~10 ⁷ cells / mL) in gilgyeong, Portulaca Oleracea, maekmundong, beam Golgi, saengjihwang, yeochunho, yeonjayuk, jisil, Cnidium, a primary extract of cheonmundong and tosaja Bifidobacteria are inoculated in a basic medium (BL medium) containing the medium, and then cultured for 24 to 120 hours at pH 6.5 to 7.5, temperature 35 to 38 ° C, and anaerobic culture conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

When fungi is used as a microorganism, fungi are preferably added to fungi in Gakyung, Maechian, McMundong, Bogolji, Yijiji, Yeonchun, By weight, and solid fermentation is carried out for 60 days at a temperature of 18 to 25 DEG C and a humidity of 60 to 70%, whereby each of the fermented extracts of medicinal plants can be obtained.

The pharmaceutical fermented extracts as described above exhibit a superior anti-inflammatory and anti-inflammatory effect when compared with common medicinal plant extracts when the anti-inflammatory effect is measured by the PGE2 generation inhibitory effect test. In addition, when the cosmetic composition is manufactured using the above-mentioned fermentation extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin and exhibits an excellent skin troubles reducing effect.

The medicinal fermentation extract obtained as described above is preferably contained in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight, in the cosmetic composition for skin troubles of the present invention. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition for relieving skin troubles containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by containing 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate, etc. in addition to the pharmaceutical fermentation extract of the present invention. In addition to the medicinal fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition for relieving skin troubles of the present invention may contain, in addition to the above-described ingredients, the components listed above in addition to the components of ordinary skin cosmetics such as oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, dye, And the like can be appropriately used in combination as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

100 g of each crushed product obtained by purchasing and grinding the product is placed in purified water or 3 L of 80% methanol, and a cooling condenser is added to each of the crushed products, which are commercially available products such as Gakyung, Maji, McMundong, Bogolji, Lt; / RTI > for 3 hours. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of medicament according to the extraction solvent.

Medicinal 80% methanol Purified water Gakyung 5.3 g 5.0 g Machi 7.8 g 7.2 g McMundong 8.1 g 7.5 g Bogor 7.1 g 6.5 g Raw persimmon 9.3 g 8.5 g Women's 8.8 g 8.2 g Mature meat 7.1 g 6.3 g House 8.3 g 8.0 g Celestial 8.5 g 7.7 g Chunmun Dong 7.9 g 7.5 g Tosa 5.5 g 5.2 g

Examples 2 to 12

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight (g) of fermentation broth Example 2 (Gakyung) 6.00 Example 3 (Mahi prefecture) 6.25 Example 4 (McMundeong) 5.92 Example 5 (Bogorji) 5.87 Example 6 (raw persimmon) 5.99 Example 7 (female spring) 5.86 Example 8 (Snack meat) 6.22 Example 9 (Support) 6.15 Example 10 (Tungkung) 5.89 Example 11 (Chunmun-dong) 5.95 Example 12 (Tozanza) 5.98

Examples 13 to 23

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight (g) of fermentation broth Example 13 (Gakyung) 5.92 Example 14 (Mahi prefecture) 6.10 Example 15 (McMundeong) 6.05 Example 16 (Bogorji) 5.87 Example 17 (raw persimmon) 5.82 Example 18 (female spring) 6.01 Example 19 (Young livestock) 6.17 Example 20 (Support) 5.92 Example 21 (Tungkung) 5.87 Example 22 (Chunmun-dong) 5.97 Example 23 (Tossa) 6.22

Examples 24 to 34

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight (g) of fermentation broth Example 24 (Gakyung) 6.15 Example 25 (Mahi prefecture) 6.15 Example 26 (Macmun Dong) 6.11 Example 27 (Bogorji) 6.03 Example 28 (raw persimmon) 6.19 Example 29 (female spring) 6.13 Example 30 (mature flesh) 5.83 Example 31 (Support) 6.03 Example 32 (Tiantai) 6.10 Example 33 (Chunmun-dong) 6.09 Example 34 (Tossa) 6.18

Examples 35 to 45

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight (g) of fermentation broth Example 35 (Gakyung) 4.92 Example 36 (Mahi prefecture) 7.02 Example 37 (McMundeong) 7.27 Example 38 (Bogorji) 6.39 Example 39 (raw persimmon) 8.23 Example 40 (female spring) 7.95 Example 41 (mature flesh) 6.33 Example 42 (Support) 7.56 Example 43 (Tiantai) 7.77 Example 44 (Chunmun-dong) 7.12 Example 45 (Tossa) 4.87

Experimental Example 1. Inhibitory effect on PGE2 production

To confirm the anti-inflammatory effect of each of the medicinal materials themselves and the medicinal fermentation extracts of Examples 2 to 45, the effect of suppressing the formation of PEG2 was tested. (Jeffrey, Kh, Anglea, SW, Zoe, S. and Rhia CM Intracellular Measurement of Prostaglandin E2: Effect of Anti-Inflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28).

Fibroblasts isolated from human epidermal tissue were subcultured several times, and then 1x10 < ⁵ > cells were placed in 96-well plates and cultured for 24 hours. FBS, and cultured for 2 hours. The respective medicinal materials themselves and the medicinal fermentation extracts of Examples 2 to 45 were treated at the concentrations shown in Table 6 below, and indomethacin was also used as a comparative germ After incubation for 1 hour, calcium ionophore A23187 and arachidonic acid were treated at a concentration of 50 mM for stimulation for 5 minutes at 37 ° C. Cells were treated with dodecyltrimethlammonium bromide for 10 minutes at a final concentration of 0.25%, and the amount of PGE2 was quantitated using an enzyme-linked immunosorbent assay (ELISA) by taking an appropriate amount of the supernatant. The prostaglandin inhibitory effect of the herbal medicine fermentation broth was evaluated, and the results are shown in Tables 6 and 7.

sample PEG increase (pg / well) % Inhibition Control (negative control) - - Arachidonic acid (positive control) 219 - Indomethacin (positive control) 14 93.6 YM badge 195 11.0 MRS badge 183 16.4 BL medium 190 13.2
Gakyung Medicinal self 152 30.6 The yeast fermented extract of Example 2 121 44.7 The lactic acid bacteria fermented extract of Example 13 115 47.5 The bifidobacterial fermentation extract of Example 24 132 39.7 The fungal fermentation extract of Example 35 120 45.2
Machi Medicinal self 148 32.4 The fermented yeast extract of Example 3 110 49.8 The fermented extract of lactic acid bacteria of Example 14 105 52.1 The bifidobacterial fermentation extract of Example 25 92 56.6 The fungal fermentation extract of Example 36 115 47.5
McMundong Medicinal self 135 38.4 The fermented yeast extract of Example 4 95 56.6 The fermented extract of lactic acid bacteria of Example 15 102 53.4 The bifidobacterial fermentation extract of Example 26 110 49.8 The fungal fermentation extract of Example 37 105 52.1
Bogor Medicinal self 149 32.0 The fermented yeast extract of Example 5 110 49.8 The lactic acid bacteria fermented extract of Example 16 103 53.0 The bifidobacterial fermentation extract of Example 27 121 44.7 The fungal fermentation extract of Example 38 101 53.9
Raw persimmon Medicinal self 130 40.6 The yeast fermented extract of Example 6 92 58.0 The fermented extract of lactic acid bacteria of Example 17 98 55.3 The bifidobacterial fermentation extract of Example 28 107 1.1 The fungal fermentation extract of Example 39 105 52.1

sample PEG increase (pg / well) % Inhibition
Women's Medicinal self 152 30.6 The yeast fermented extract of Example 7 21 44.7 The fermented extract of lactic acid bacteria of Example 18 113 48.4 The bifidobacterial fermentation extract of Example 29 107 51.1 The fungal fermentation extract of Example 40 117 46.6
Mature meat Medicinal self 166 24.2 The yeast fermented extract of Example 8 130 40.6 The fermented extract of lactic acid bacteria of Example 19 112 48.9 The bifidobacterial fermentation extract of Example 30 102 53.4 The fungal fermentation extract of Example 41 113 48.4
House Medicinal self 138 37.0 Example 9 Yeast Fermentation Extract 89 59.4 The fermented extract of lactic acid bacteria of Example 20 95 56.6 The bifidobacterial fermentation extract of Example 31 101 53.9 The fungal fermentation extract of Example 42 107 51.1
Celestial Medicinal self 130 40.6 The yeast fermented extract of Example 10 92 58.0 The fermented extract of lactic acid bacteria of Example 21 97 55.7 The bifidobacterial fermentation extract of Example 32 102 53.4 The fungal fermentation extract of Example 43 107 51.1
Chunmun Dong Medicinal self 147 32.9 The fermented yeast extract of Example 11 113 48.4 The lactic acid bacteria fermented extract of Example 22 107 51.1 The bifidobacterial fermentation extract of Example 33 103 53.0 The fungal fermentation extract of Example 44 118 46.1
Tosa Medicinal self 150 31.5 The fermented yeast extract of Example 12 115 47.5 The fermented extract of lactic acid bacteria of Example 23 126 42.5 The bifidobacterial fermentation extract of Example 34 103 53.0 The fungal fermentation extract of Example 45 109 50.2

As shown in Tables 6 and 7, the medicinal fermented extracts obtained according to Examples 2 to 45 exhibited higher inhibition rates of prostaglandin (PGE2) than those of the respective medicines themselves or the basic medium, thereby suppressing induction or inhibition of COX-2 enzyme It was found that the anti-inflammatory effect was excellent.

The following are examples of preparation of cosmetic composition by using the fermented extract of medicinal substance according to the present invention which is excellent in anti-inflammatory and antiperspirant effect and excellent in skin troubles-relieving function and safe to skin.

Example 46 and Comparative Example 1

Using the fermented extract of Ganoderma lucidum of Example 35, ointment for external use on skin was prepared as shown in Table 8 below.

division Example 46 Comparative Example 1 Gyungyung mold fermentation extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 47 and Comparative Example 2

Using the fermented extract of Ganoderma lucidum of Example 35, a cream was prepared according to the prescription of Table 9 below.

division Example 47 Comparative Example 2 Gyungyung mold fermentation extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 48 and Comparative Example 3

Using the fermented extract of Ganoderma lucidum of Example 35, the softening longevity was prepared according to the formulation shown in Table 10 below.

division Example 48 Comparative Example 3 Gyungyung mold fermentation extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 49 and Comparative Example 4

Using the fermented extract of Ganoderma lucidum of Example 35, a nutritional lotion was prepared according to the prescription of Table 11 below.

division Example 49 Comparative Example 4 Gyungyung mold fermentation extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 50 and Comparative Example 5

Using the fermented extract of Ganoderma lucidum of Example 35, a pack was prepared according to the prescription of Table 12 below.

division Example 50 Comparative Example 5 Gyungyung mold fermentation extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 51 and Comparative Example 6

Using the fermented extract of Ganoderma lucidum of Example 35, an essence was prepared according to the prescription of Table 13 below.

division Example 51 Comparative Example 6 Gyungyung mold fermentation extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example 2. Anti-inflammatory effect

To investigate the anti-inflammatory effect of the cosmetic preparation prepared in Example 46, a method using arachidonic acid (A. Crummey, GP Harper, EA Boyle and FR Mangan, Inhibitory of arachidonic acid-induced ear edema as a model for assessing Topical anti-inflammatory compound (Agents and Actions, 1987, 20: 69-76).

First, 35 mice (hairless mice) were divided into 7 animals. Both ears were cleaned with ethanol before application of the sample, and then measured using a micrometer. The cosmetic compositions of Example 46 and Comparative Example 1, indomethacin indomethacin) 1.0% and a control group which treated only arachidonic acid, and the average value of the ear thickness of the mouse was measured. For the cosmetic preparation of Example 46, the cosmetic preparation of Comparative Example 1, and the comparative group for treatment of 1.0% indomethacin, 20 μl of the sample and 20 μl of ethanol were continuously applied for 1 day once a day for 4 days. After the last application and curing for 1 hour, ethanol was applied to the left ear and arachidonic acid was applied to the right ear at 2 mg / ear. After 1 hour of application, the degree of edema of the ear was repeated three times for both ears using a micrometer Respectively. The left ear of the treated group was used as a control group to examine the degree of inflammation induced by the sample itself. The anti-inflammatory effect was evaluated based on the control group treated with arachidonic acid alone. The results are shown in Table 14 below.

The inhibition rate was measured according to the following equation (1).

[Equation 1]

A is the average thickness change of the control ear (the thickness of the arachidonic acid treatment pad - the thickness of the untreated arachidonic acid ear), B is the average thickness change of the sample application group ear (the thickness of the treated ear, to be.

division Concentration (%) Glume (㎛) % Inhibition After sample treatment Before sample treatment Sample fungus Example 46 - 335 533 25.8 Comparison group 1 Comparative Example 1 - 350 559 12.9 Comparison group 2 Indomethacin 1.0 357 497 41.7 Control group Arachidonic acid 2 mg / ear 333 573 -

As shown in Table 14, the anti-inflammatory effect of Example 46 according to the present invention was 25.8%, which is lower than that of indomethacin, which is a highly effective anti-inflammatory drug, As shown in FIG.

The cosmetic composition for relieving skin troubles according to the present invention is excellent in skin-relieving effect and persistence of effect by enhancing the pharmacological effect of each medicinal substance through fermentation while enhancing the anti-inflammatory effect.

Claims (11)

A cosmetic composition for alleviating skin troubles, which comprises as an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of Gamyung, McMundong, Bogolji, Beegeopgang, Yeonchunho, The method according to claim 1, Wherein the fermentation extract is obtained by culturing yeast, lactic acid bacteria or bifidobacteria with a medicament. 3. The method of claim 2, Wherein the fermentation extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. 3. The method of claim 2, Wherein the yeast is selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids, and Saccharomyces boulardii . 3. The method of claim 2, Wherein the lactic acid bacteria are selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis . 3. The method of claim 2, Wherein the bifidobacteria are selected from the group consisting of Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis . The method according to claim 1, Wherein the fermentation extract is obtained by inoculating fungi into a medicinal material and solid fermentation. 8. The method of claim 7, Wherein the fungus is selected from the group consisting of Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , phellinus linteus and Ganoderma lucidum. / RTI > The method according to claim 1, Wherein the medicinal fermentation extract is contained in the cosmetic composition in an amount of 0.0001 to 10% by weight. The method according to claim 1, Wherein the cosmetic composition is a formulation of ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, or oil gel. The method according to claim 1, Wherein the cosmetic composition further comprises at least one additive selected from the group consisting of oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative and a flavoring agent Composition.