KR101354412B1 - Cosmetic composition for improving atopic dermatitis and skin whitening comprising ferment filtrate of Ledum palustre var. diversipilosum and manufacturing method thereof - Google Patents

Abstract

The present invention is a cedar leaf ( Ledum palustre var. The present invention relates to a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting the extract of diversipilosum) with lactic acid bacteria, and a method for preparing the same. Preferably, the present invention relates to a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting a nano extract containing cedar leaf nanoparticles with lactic acid bacteria, and a method for producing the same.

Description

Cosmetic composition for improving and whitening atopic dermatitis including fermented filtrate and method for preparing the same {Cosmetic composition for improving atopic dermatitis and skin whitening comprising ferment filtrate of Ledum palustre var. diversipilosum and manufacturing method

The present invention is a cedar leaf ( Ledum palustre var. The present invention relates to a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting the extract of diversipilosum) with lactic acid bacteria, and a method for preparing the same. Preferably, the present invention relates to a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting a nano extract containing cedar leaf nanoparticles with lactic acid bacteria, and a method for producing the same.

Due to the advancement of the Korean economy, Western skin diseases such as atopic dermatitis are gradually increasing, which is becoming a big social issue. Atopic dermatitis is a chronic skin disease that is common in children and continues to manifest in adults.Atopic dermatitis is on the rise worldwide. Recently, atopic dermatitis is more common in children and more than 20% in children and 1-3% in adults. It became. To date, numerous studies have been conducted on the causes of atopic dermatitis, but it has not been fully identified until now, but it is estimated that atopic dermatitis occurs as a result of complex factors and various immunological reactions as well as various clinical features. Predisposition and environmental factors, immunological adverse events and abnormal skin barriers are thought to be the main causes. In the case of atopic dermatitis, the treatment of oriental medicine and bilateral medicine is different, and it is difficult to provide accurate treatment guidelines for patients who are medical consumers and their families.

Patients with atopic dermatitis are generally known to be more susceptible to skin infections caused by bacteria, viruses, and fungi than normal people. In particular, skin infections caused by Staphylococcus aureus frequently occur. Staphylococcus aureus is cultured about 95% in the skin of patients with atopic dermatitis, but normal people are cultured about 5%, indicating a close relationship with the occurrence of atopic dermatitis. In the case of atopic dermatitis patients, the innate antimicrobial peptides present in the skin are less or less functional than normal people, and bacteria are known to multiply well on the skin.

Topical steroids have been used as the most basic and effective treatment for atopic dermatitis for over 50 years, and currently there is no cure as effective as topical steroids. However, long-term use may cause side effects such as swelling in the right area, skin atrophy, capillary expansion, steroidal acne, hirsutism, and purpura. As these side effects are recently reported through newspapers, magazines and the Internet, many patients with atopic dermatitis or carers are reluctant to use topical steroids (steroid phobia). Therefore, it is necessary to identify the causes of atopic dermatitis and to develop a more fundamental treatment based on this.

In addition, atopic dermatitis causes pigmentation on the skin, causing the skin to darken, thereby increasing the pain of patients with atopic dermatitis. Therefore, it would be more desirable to develop a new material having a whitening effect on post-inflammatory hyperpigmentation as well as the therapeutic effect of atopic dermatitis.

On the other hand, Red Palumre is native to Korea, China, Japan, and Russia, and is an evergreen shrub of 10 to 50 cm tall azaleas distributed in the altitude of 1,700 ~ 2,700m above Mt. It is reported that it is used as a medicine to treat bronchitis, hepatitis, acute rhinitis, asthma, dysmenorrhea and infertility in Korean medicine, and it is reported that it is used as a raw material for blood pressure lowering agents in Japan (Korean Patent Application No. 10-2002-0063286) . In addition, it has been reported that cedar can be used for the symptomatic treatment of rheumatism, arthrosis and insect bites (Dubois, et al., 1990, phytochemistry, Vol. 29, No. 10, pp.3369-3371), Korean patent In application 10-2003-0042520, the two lobes ( Ledum palustre There is L. It has been reported that angustum N. Busch) extract has excellent anti-inflammatory and antioxidant activity.

The present inventors conducted a study to search for substances having an effect of improving and whitening atopic dermatitis, and the result of fermentation of cedar leaves by fermentation of cedar leaf extracts with lactic acid bacteria was applied to the skin whitening due to improvement of atopic dermatitis and atopic dermatitis. It was confirmed that there is an excellent effect to complete the present invention.

One object of the present invention is a cedar leaf ( Ledum palustre var. It is to provide a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting diversipilosum) extract with lactic acid bacteria.

Another object of the present invention to provide a method for producing the cosmetic composition.

As one embodiment for achieving the above object, the present invention relates to a cosmetic composition for improving and whitening atopic dermatitis, including a cedar leaf fermentation filtrate produced by fermenting the extract of Ledum palustre var. Diversipilosum with lactic acid bacteria .

Preferably, the lactic acid bacterium is Enterococcus faecium), Enterococcus faecalis (Enterococcus faecalis ), Lactobacillus plantarum , Lactobacillus permentum fermentum , Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei , Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella < RTI ID = 0.0 > hellenica), Lactococcus taught rain (Lactococcus garvieae), Peddie Oh Caucus pen Tosa three mouse (Pediococcus pentosaceus), when the CD rakti Peddie Oh Caucus (Pediococcus acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis ) and Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > infantis ) may be two or more mixed strains selected from the group consisting of.

Also preferably, the enterococcus paesum ( Enterococcus faecium ) enterococcus faecium ) ATCC 19634 or Enterococcus faecium ) at least one selected from ATCC 6057,

The Enterococcus faecalis ) is Enterococcus faecalis ATCC 6057,

Lactobacillus plantarum plantarum) is Lactobacillus Planta Room (Lactobacillus plantarum ) at least one selected from ATCC 10241, or Lactobacillus plantarum NCTC 7220,

The Lactobacillus < RTI ID = 0.0 > fermentum ) is Lactobacillus fermentum ATCC 14931, or Lactobacillus fermentum ) at least one selected from NCDO 1750,

Lactobacillus lactis ) is Lactobacillus lactis ) ATCC 11454, or Lactobacillus lactis ) one or more selected from NCIB 8586,

The Lactobacillus < RTI ID = 0.0 > rhamnosus) is Lactobacillus ramno suspension (Lactobacillus rhamnosus) ATCC 7469, or Lactobacillus ramno suspension (Lactobacillus rhamnosus ) one or more selected from KCTC 3326,

Lactobacillus sakei ) Lactobacillus sakei ) ATCC 31063, or Lactobacillus sakei ) is one or more selected from ATCC 15521,

Lactobacillus brevis ( Lactobacillus) brevis ) is Lactobacillus brevis ATCC 15521,

Lactobacillus Graminis ( Lactobacillus) graminis ) is Lactobacillus graminis ATCC 51150,

Leuconostoc Citrium citreum ) is Leuconostoc citreum ATCC 49370,

The Bisella Hellenica ( Weissella) hellenica is a bisella Helena ( Weissella) hellenica ) ATCC 51523,

Lactococcus garvieae) it is Lactococcus (in the non-teaching Lactococcus garvieae) CCUG 11672, (Lactococcus in Lactococcus teaching ratio garvieae ) ATCC 11454, or Lactococcus garvieae ) at least one selected from LMG 8162,

Pediococcus pentosaceus pentosaceus ) is Pediococcus pentosaceus ATCC 33316,

Pediococcus ecidi lactici ( Pediococcus acidilactici ) is Pediococcus acidilactici ATCC 33314,

The Bifidobacterium animalis ( Bifidobacterium) animalis ) is Bifidobacterium animalis ATCC 27672,

Bifidobacterium Infantis infantis) may be Bifidobacterium Infante Tees (Bifidobacterium infantis) ATCC15697.

Also preferably, the composition of the present invention may comprise bacteriocin produced by said lactic acid bacteria.

More preferably, the lactic acid bacteria in the present invention is Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus rhamnosus ( Lactobacillus) rhamnosus ), Lactococcus garvieae , and Pediococcus pentosaceus ( Pediococcus) pentosaceus ) may be a mixed strain of two or more selected from the group consisting of.

Also more preferably, the Lactobacillus plantarum ( Lactobacillus plantarum ) is Lactobacillus plantarum ( Lactobacillus plantarum ) ATCC 10241, or Lactobacillus plantarum ) at least one selected from NCTC 7220,

The Lactobacillus < RTI ID = 0.0 > rhamnosus) is Lactobacillus ramno suspension (Lactobacillus rhamnosus) ATCC 7469, or Lactobacillus ramno suspension (Lactobacillus rhamnosus ) one or more selected from KCTC 3326,

Lactococcus garvieae) it is Lactococcus (in the non-teaching Lactococcus garvieae) CCUG 11672, (Lactococcus in Lactococcus teaching ratio garvieae ) ATCC 11454, or Lactococcus garvieae ) at least one selected from LMG 8162,

Pediococcus pentosaceus pentosaceus ) may be Pediococcus pentosaceus ATCC 33316.

Also more preferably, the lactic acid bacteria of the present invention is Lactobacillus plantarum Lactobacillus plantarum ) ATCC 10241, Lactobacillus rhamnosus ) ATCC 7469 or Pediococcus pentosaceus pentosaceus) as in any one of bacteria and Lactococcus teaching ratio selected from ATCC 33316 (Lactococcus garvieae ) ATCC 11454, or Lactococcus garvieae ( Lactococcus garvieae ) may be composed of any one selected from LMG 8162.

Also preferably, the cedar leaf extract of the present invention may be a nano extract containing the nanoparticles of cedar leaf.

Also preferably, the nanoparticles of the cedar leaves may be homogenized particles in the range of 100 to 500nm.

Also, preferably, the cosmetic composition of the present invention may contain one formulation selected from the group consisting of softening cream, nutrient cream, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. It may be to have.

As another aspect, the present invention is a cedar leaf ( Ledum palustre var. diversipilosum) to prepare a fennel leaf nano extract containing nanoparticles;

Mixing the cedar leaf nano extract into the lactic acid bacteria culture medium; And

Enterococcus on the lactic acid bacteria culture medium ( Enterococcus faecium), Enterococcus faecalis (Enterococcus faecalis , Lactobacillus plantarum), Lactobacillus spread lactofermentum (Lactobacillus fermentum), Lactobacillus lactis (Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei , Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella < RTI ID = 0.0 > hellenica), Lactococcus taught rain (Lactococcus garvieae), Peddie Oh Caucus pen Tosa three mouse (Pediococcus pentosaceus), when the CD rakti Peddie Oh Caucus (Pediococcus acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis ) and Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > infantis ) relates to a method for producing a cosmetic composition of claim 1 comprising the step of inoculating and fermenting two or more lactic acid bacteria selected from the group consisting of.

Preferably, the temperature at the time of incubation of the lactic acid bacteria is usually 25 ℃ to 40 ℃, the incubation time is 7 hours to 40 hours, the lactic acid bacteria may be used to concentrate to 10 ¹⁰ to 10 ¹¹ CFU / ml.

Also preferably, the cedar leaf nanoparticles may be homogenized particles in the range of 100 to 500nm.

Also preferably, the cedar leaf nano extract may be included in 1 to 2% by weight compared to the lactic acid bacteria culture medium.

Hereinafter, the present invention will be described in more detail.

As used herein, the term "fennel leaf extract" means an extract obtained from a leaf of cedar. As the extraction method, a conventional extraction method can be used, and methods such as extraction with an extraction solvent, supercritical fluid extraction, ultrasonic extraction, and hot water extraction can be exemplified, but the present invention is not limited thereto.

As used herein, the term "produced by fermentation with lactic acid bacteria" refers to the denaturation or modification of raw materials by physical or chemical methods using lactic acid bacteria. In natural terms, the term "fermented by lactic acid bacteria" It means the decomposition process that can get the result. In particular, fermentation using lactic acid bacteria in the present invention means that lactic acid bacteria selectively extract and release only the weakness and components contained in the raw material without changing the external shape.

As used herein, the term "fermentation filtrate" is a filtrate obtained by removing the lactic acid bacteria cells from the medium in which the cedar leaf extract is fermented with lactic acid bacteria, and includes components included in the medium in which the cedar leaf extract is fermented and cultured. Preferably, the cosmetic composition according to the present invention comprises a nano fermentation filtrate produced by mixing the cedar leaf nano extract in lactic acid bacteria culture medium and inoculated and fermented lactic acid bacteria.

In the present invention, lactic acid bacteria used for fermentation of the extract of cedar leaves are bacteria that produce lactic acid by decomposing sugars such as glucose. The lactic acid produced by lactic acid fermentation prevents the growth of pathogens and harmful bacteria in food production such as dairy products, kimchi and brewed foods. In addition, it is a bacterium that is used as a formal medicine by inhabiting the intestines of mammals and preventing abnormal fermentation by various bacteria.

The lactic acid bacteria to be used in the present invention are preferably Enterococcus faecium), Enterococcus faecalis (Enterococcus faecalis ), Lactobacillus plantarum , Lactobacillus permentum fermentum), Lactobacillus lactis (Lactobacillus lactis), Lactobacillus Caucasus ramno (Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei), Lactobacillus brevis (Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella hellenica , Lactococcus garvieae , Pediococcus pentosaceus ), Pediococcus ( Pediococcus) acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis ) and Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > infantis ). < / RTI > The microorganisms used in the present invention are known bacteria and are available from the BRCs of the Korea Research Institute of Bioscience and Biotechnology, the Belgian Co-ordinated Collections of Microorganisms (BCCM) or the American Type Culture Collection (ATCC). More specifically, the bacteria used in the present invention include enterococcus Examples of faecium include enterococcus faecium faecium ) ATCC 19634 or Enterococcus faecium ) You can select one or more of ATCC 6057 and enterococcus faecalis ) Enterococcus faecalis ) can be used ATCC 6057, Lactobacillus plantarum Lactobacillus plantarum ( Lactobacillus plantarum ) ATCC 10241, or Lactobacillus plantarum ) NCTC 7220 can be used to select one or more, Lactobacillus fermentum ) Lactobacillus fermentum ) ATCC 14931, or Lactobacillus fermentum ) NCDO 1750 can be selected and used, Lactobacillus Lactobacillus lactis ) may include Lactobacillus lactis ATCC 11454, or Lactobacillus lactis ) One or more of NCIB 8586 can be selected and used, Lactobacillus rhamnosus ) Lactobacillus rhamnosus ) ATCC 7469, or Lactobacillus rhamnosus KCTC 3326 can be used to select one or more, and Lactobacillus Sakei ( Lactobacillus) sakei ) Lactobacillus sakei ) ATCC 31063, or Lactobacillus sakei ) can be used to select one or more of ATCC 15521, Lactobacillus brevis ) Lactobacillus brevis ) ATCC 15521 can be used and the Lactobacillus graminis ) Lactobacillus graminis ) ATCC 51150 can be used, and Leuconostoc citreum is Leuconostoc citreum ) can be used ATCC 49370, Weissella hellenica ) Weissella, Bisella hellenica ) can use ATCC 51523, Lactococcus garvieae) roneun in Lactococcus teaching taught in (Lactococcus garvieae) CCUG 11672, Lactococcus Rain (Lactococcus garvieae ) ATCC 11454, or Lactococcus garvieae ) LMG 8162 can be used to select one or more, Pediococcus pentosaceus ( Pediococcus pentosaceus) roneun Phedi O Lactococcus pen soil three mouse (Pediococcus pentosaceus) ATCC 33316 may be used, when the O Phedi Lactococcus CD rakti (Pediococcus acidilactici) roneun when the CD rakti Phedi O Rhodococcus (Pediococcus acidilactici ) ATCC 33314 can be used and the Bifidobacterium anmalis ( Bifidobacterium) animalis) roneun Bifidobacterium No Marlies (Bifidobacterium animalis) can be used to ATCC 27672, Bifidobacterium Infante Tees (Bifidobacterium infantis) roneun Bifidobacterium Infante Tees (Bifidobacterium infantis) can be used ATCC15697.

The cosmetic composition of the present invention preferably comprises the bacteriocin produced by the lactic acid bacteria, bacteriocin is an antimicrobial peptide or protein, which is a substance that kills or inhibits growth of related bacteria, and is directly biosynthesized by an inherent gene (ribosomal). It is easily degraded by proteolytic enzymes, which is harmless to humans and has no residual. The type of bacteriocin included in the culture according to the present invention is not particularly limited, and specific types thereof include nisin, subtilin, bacillisin, colicins, and alveicins. ), Cartovoricins, arizonacins, cloacins, magescins, pneumocins, aerocins, pyocins , Fluocins, pesficins, megacins, monocins, cerecins, enterrococcins and staphylococcins.

The cosmetic composition of the present invention can be used for improving atopic dermatitis.

In addition, the cosmetic composition of the present invention can provide a whitening effect on the skin in which the pigment is deposited by atopic dermatitis.

Cosmetic composition in the present invention is characterized in that the functional cosmetic composition. The functional cosmetic composition means a cosmetic composition prepared by emphasizing specific functions for the management and prevention of specific skin or a cosmetic composition for prevention and proper management of various skin diseases caused by genetic and environmental factors.

The cosmetics of the present invention are creams, packs, lotions, essences, lotions, foundations and makeup bases, etc., and may be prepared and commercialized in any of these formulations to achieve the object of the present invention. It is not limited to. Preferably, the cosmetic composition may include one formulation selected from the group consisting of softening cream, nourishing cream, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder have.

In addition, the cosmetic composition according to the present invention may optionally add purified water, humectant, higher alcohol, higher fatty acid, emollient, chelating agent, thickener, permanent ingredient, pH adjusting agent, flavoring agent, etc. according to the formulation or purpose of use of the cosmetic. have.

Looking at the cosmetic preparation method containing the fermentation filtrate of the extract of cedar leaves according to the present invention,

1 duhyang lobe (Ledum palustre var. diversipilosum) to prepare a fennel leaf nano extract containing nanoparticles;

(2) mixing the cedar leaf nano extract into the lactic acid bacteria culture medium; And

(3) Enterococcus par when Titanium (Enterococcus faecium) to the culture medium of lactic acid bacteria, Enterococcus faecalis (Enterococcus faecalis , Lactobacillus plantarum , Lactobacillus < RTI ID = 0.0 > fermentum , Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei , Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella < RTI ID = 0.0 > hellenica), Lactococcus taught rain (Lactococcus garvieae), Peddie Oh Caucus pen Tosa three mouse (Pediococcus pentosaceus), when the CD rakti Peddie Oh Caucus (Pediococcus acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis ) and Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > infantis ) consists of inoculating and fermenting two or more lactic acid bacteria selected from the group consisting of.

Step (1) is a step of preparing the cedar leaf nano extract, in the present invention, in order to overcome the skin absorption and raw material stability problems of the cedar leaf extract raw material itself, by fusing nanotechnology (nanotechnology) superior safety than conventional bio materials It provides a nano-extract fermentation filtrate functional cosmetic material with good absorption and mobility.

In the present invention, the cedar leaf nano extract is preferably prepared by ultrasonic extraction after grinding the cedar leaf with a nano crusher (Nanomil, Simbex. Co., Ltd, German), the size of the nanoparticles of 100 ~ 500nm range It is preferably a homogenized particle. In order to prepare the nano-extract, it is preferable to make the nanoparticles after the preparation of the powder of the cedar leaves, the powder is made by physically grinding using a nano crusher (Nanomil, Simbex Co., Ltd, German). The nanopowder of the cedar leaves may use a conventional method of nanoparticle granulation of food materials known in the art, and preferably, a supercritical fluid extraction process may be used. The supercritical fluid extraction process can be widely used in pharmaceuticals, foods, cosmetics, etc., because it has no toxic solvent or surfactant in the particles compared to other extraction processes. RESS (Rapid Expansion of Supercritical Solutions), GAS (Gas Anti-Solvent) (SAS), Aerosol Solvent Extraction System (ASES), Solution Enhanced Dispersion by Supercritical Fluids (SEDS), Particles from Gas Saturated Solutions (PGSS), and Reactive Precipitation in Supercritical Solution (RPSS).

In the step (2), the fermented leaf nano extracts are mixed in the lactic acid bacteria culture medium for fermentation of lactic acid bacteria. The culture medium for lactic acid bacteria may be a conventional medium known in the art. The medium may comprise various carbon sources, nitrogen sources and trace element components. Examples of carbon sources that can be used include carbohydrates such as glucose, sucrose, lactose, fructose, maltose, starch and cellulose, fats such as soybean oil, sunflower oil, castor oil, coconut oil, fatty acids such as palmitic acid, stearic acid, linoleic acid, Alcohols such as glycerol and ethanol, and organic acids such as acetic acid. These carbon sources may be used alone or in combination. Examples of nitrogen sources that may be used include organic nitrogen sources such as peptone, yeast extract, gravy, malt extract, corn steep liquor, and soybean wheat, and inorganic nitrogen sources such as urea, ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate, . These nitrogen sources may be used alone or in combination. The medium may include potassium dihydrogen phosphate, dipotassium hydrogen phosphate and the corresponding sodium-containing salts as a person. It may also include metal salts such as magnesium sulfate, manganese sulfate or iron sulfate. In addition, sodium acetate, ammonium stearate, calcium chloride, amino acids, vitamins and suitable precursors may be included. During the culture, the pH can be adjusted by adding compounds such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid and sulfuric acid in a suitable manner. Preferably, Lactobacillus culture media include MRS (de Man, Rogosa and Sharpe) liquid medium, DMEM (Dulbecco's Modified Eagle's Medium), MEM (Minimal Essential Medium), BME (Basal Medium Eagle), RPMI 1640, F- 12, α-MEM (α-Minimal Essential Medium), G-MEM (Glasgow's Minimal Essential Medium) and Isocove's Modified Dulbecco's Medium.

In step (3), fermentation refers to a general process in which a microorganism changes its organic substance while decomposing. Through such a fermentation process, the microorganism selectively extracts the effective ingredient contained in the raw material of the herbal medicine, These metabolites are called fermentation broth.

Lactic acid bacteria for fermentation of lactic acid bacteria are Enterococcus faecium), Enterococcus faecalis (Enterococcus faecalis , Lactobacillus plantarum , Lactobacillus < RTI ID = 0.0 > fermentum , Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei , Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella < RTI ID = 0.0 > hellenica), Lactococcus taught rain (Lactococcus garvieae), Peddie Oh Caucus pen Tosa three mouse (Pediococcus pentosaceus), when the CD rakti Peddie Oh Caucus (Pediococcus acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis ) and Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > infantis ) can be used. The lactic acid bacteria may be preferably used concentrated to 10 ¹⁰ to 10 ¹¹ CFU / ml.

In the present invention, the cultivation and fermentation of the lactic acid bacteria can be carried out according to a suitable culture medium and culture conditions known in the art. Such a process can be easily adjusted by those skilled in the art according to the selected strain.

During the culture, bubbles can be inhibited by using an expanded body such as fatty acid polyglycol ester. In addition, oxygen or an oxygen-containing gas (e.g., air) may be injected to maintain the exhalation state. The temperature at the time of culturing the lactic acid bacteria is usually from 25 to 40 DEG C, preferably from 35 to 40 DEG C, and preferably from 7 to 40 hours. The lactic acid bacteria preferably have a concentration of from 10 ¹⁰ to 10 ¹¹ CFU / ml Can be used.

By fermentation using these lactic acid bacteria, the polymer which can not be absorbed into the skin due to its large size is decomposed into small molecules by the action of lactic acid bacteria. During the decomposition process, the activity of active ingredients is increased by various enzymes of lactic acid bacteria, There is also an effect of height. In addition, toxic substances such as pesticides and heavy metals can be decomposed or inactivated to remove elements that are harmful to the skin. After the fermentation process, lactic acid bacteria are finely decomposed, digested and excreted after constituting the molecules of the cedar leaf nano extract. Since the fermentation process of the present invention is carried out at a low temperature, lactic acid bacteria can not effectively lose the appearance of the raw material at room temperature without effectively losing or modifying components caused by conventional hot water extraction or vacuum extraction, have.

The cultivation and fermentation of the lactic acid bacterium can be carried out according to a suitable culture medium and culture conditions known in the art. Such a process can be easily adjusted by those skilled in the art according to the selected strain. These various methods are disclosed in various documents. Suspension cultures and adherent cultures can be divided into batches, fed-batch and continuous-batch cultures according to the culture method. During the culture, bubbles can be inhibited by using an expanded body such as fatty acid polyglycol ester. Also, oxygen or an oxygen-containing gas (e.g., air) may be injected to maintain the exhalation state. The temperature at the time of culturing is usually 25 ° C to 40 ° C, preferably 35 ° C to 40 ° C, and the culturing time may be 7 hours to 40 hours. The fennel leaf nano extract is preferably contained 1% to 2% by weight compared to the lactic acid bacteria culture medium.

After completion of the incubation, the culture can be subjected to a process of contrifugation or filtration in order to separate the cells from the culture, and these steps can be carried out as required by those skilled in the art. At this time, the removal of the cells indicates that the lactic acid bacterium cells have been substantially removed through the conventional method for removing the cells.

The cosmetic composition formed by the above manufacturing method has one formulation selected from the group consisting of flexible cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder It is preferable.

The present invention can be utilized as a supplement or an alternative means for the treatment of atopic dermatitis by using the fermentation filtrate of the cedar leaf extract, and can quickly penetrate the efficacy of the active ingredient deep into the skin through the nano method.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

1. to implementation Cedar leaf  Lactic acid bacteria of extract Fermentation filtrate  Produce

The dried cedar leaf powder was pulverized with a nano grinder (Nanomil, Simbex. Co., Ltd, German) and then ultrasonically extracted to obtain homogenized nanoparticles in the range of 100-500 nm. The cedar leaf nano extract was added to the medium based on the MRS medium to adjust the pH to 5.5, and then sterilized for 10 minutes at 100 ° C. or higher using an autoclave. After sterilization, the sample was cooled to 42 ° C., and then inoculated with 2% of the lactic acid bacteria mixed strain and fermented in a thermostat at 37 ° C. for 15 hours. A mixture of lactic acid bacteria strain is Lactobacillus Mixed strains of plantarum ATCC 10241 and Lactococcus garvieae ATCC 11454 (Preparation Example 1), Lactobacillus Mixed strain of plantarum ATCC 10241 and Lactococcus garvieae LMG 8162 (Preparation Example 2), Lactobacillus Mixed strain of rhamnosus ATCC 7469 and Lactococcus garvieae ATCC 11454 (Preparation 3), Lactobacillus Mixed strains of rhamnosus ATCC 7469 and Lactococcus garvieae LMG 8162 (Preparation 4), Lactococcus Mixed strains of Garvieae ATCC 11454 and Pediococcus pentosaceus ATCC 33316 (Preparation 5) were used, respectively. Lactococcus garvieae LMG 8162 was sold by Belgian Co-ordinated Collections of Micro-organisms (BCCM) and all others were used by American Type Culture Collection (ATCC).

35 kg of the culture medium was prepared in the same manner as the culture medium in the fermenter, and inoculated with 3.5 kg of the pre-culture solution, followed by incubation at 30-37 ° C. for 12-20 hours to prepare the culture medium. After the completion of the incubation, the temperature of the fermenter was raised to about 70 ° C. or more and maintained for 30 minutes to inactivate the bacteria. After cooling the temperature of the fermentor to about 50 ℃, the culture was centrifuged to separate the cells and the culture filtrate. Activated charcoal corresponding to about 1.0 to 2.0% of the total volume of the culture filtrate was added to the separated culture filtrate, and decolorized and deodorized for about 3 hours, which was composed of a filter press (diatomaceous earth + filter cloth); 55 ~ 60 ℃ filter press pressure is about 1 ~ 2 atm, pore size of filter cloth is about 5㎛, diatomaceous earth use is about 5% of total volume of filtrate to be treated) to remove activated carbon and impurities Culture filtrates were obtained. The purified culture filtrate was concentrated to yield about 3.5 kg of concentrated culture filtrate with a solid concentration of about 20 Brix. The concentrated culture filtrate was lyophilized at a trap temperature of about −80 ° C. or lower for about 40 hours or longer to obtain a dry powder of the fermentation filtrate.

Example  2. Cedar leaf  Lactic acid bacteria of extract Fermentation filtrate  Containing Cosmetics  Manufacturing of formulations

2-1. Manufacturing of Moisturizing Softeners

The moisturizing softener is A phase lactic acid bacteria fermentation filtrate 76.198%, EDTA-2Na (disodium ID) 0.05%, D, L-Panthenol (Delpanthenol) 0.10%, Aminocoat 2.00%, Allantoin 0.10%, Glycerin 7.00%, Na-Hyaluronate (1%, Sodium Hyaluronate) 3.00%, PCA-Na (2-pyrrolidone-5- Sodium carbonate) 4.00%, 1,3-BG (butylene glycol) 4.00%, Carbopol 941 (carbomer) 0.07%, Aronvis-M (Aronvis) 0.05%, Ethanol (95 %, Ethanol) 1.50%, Spectrastat 0.30%, Nikkol HCO-40 0.30%, DC 556 0.20%, FE 6014245 0.05%, Phase C contains TEA 0.07%, Complex plant extract 1.00%, Caramel pigment 0.012% do. Looking at the manufacturing process of the moisturizing softener using the above raw materials, 1) put the A phase in the main kiln and completely dissolve it, 2) dissolve the B phase in a separate dissolution tank, and 3) put the C phase in the main kiln Moisturizing softener was prepared by placing and dissolving completely.

2-2. Moisturizing Serum

Moisturizing Serum is A. Lactobacillus Fermentation Filtrate of Purified Leaf + Perilla Leaf Extract Prepared in Example 1 as Phase A, 0.05% EDTA-2Na (Disodium IDT), 0.10% D, L-Panthenol (Delpanthenol), Aminocoat (Aminocoat) 1.00%, PCA-Na (2-pyrrolidone-5-sodium carbonate) 3.00%, Glycerin (glycerine) 8.00%, Adenosine (adenosine) 0.04%, 1,3-BG (butylene glycol (Col) 10.00%, oil-soluble licorice extract 0.05%, Keltrol-F (Keltrolf) 0.10%, Natrosol 250HR 0.26%, as phase B Squalane (Squalane) 1.50%, Grape seed oil (Grape seed oil) 1.20%, Tegocare 450 1.30%, Abil EM 90 (Cetyl Dimethicone Copolyol) 0.50%, Nikkol HCO-40 0.60%, Spectrastat 0.30%, Neosil ES 8.00%, Sepigel 305 0.40%, Na-Hyaluronate (1) %, Sodium hyaluronate) 3.00%, FE 6014245 0.10%, composite plant extract 1.00%, caramel pigment 0.07%. Looking at the manufacturing process of the moisturizing serum using the above raw materials, 1) put the A phase in the main kiln and completely dissolve it, 2) dissolve the B phase in a separate dissolution tank, and 3) put the C phase in the main kiln Moisturizing serum was prepared by placing and dissolving completely.

2-3. Moisturizing Emulsion

Moisturizing Emulsion is Phase A lactic acid bacterium fermentation filtrate prepared in Example 1 + Purified water + Phase A, EDTA-2Na 0.05%, D, L-Panthenol 0.10%, Aminocoat 2.00%, PCA-Na (2-pyrrolidone-5-sodium carbonate) 5.00%, Glycerin 7.00%, Adenosine (adenosine) 0.04%, 1,3-BG (butylene article) Lycol) 4.00%, oil-soluble licorice extract 0.05%, Carbopol 941 (carbomer) 0.04%, Aronvis-M (Aronvis) 0.02%, Lanette O (cetearyl alcohol) 1.50% as phase B, Montanov L (Montawax) 0.80%, Arlacel 165 1.50%, Tween 60V 0.80%, Spectrastat 0.30%, CEH 5.00%, Tegocare 450 0.70%, Olivoil Glutinate 0.80%, Shea Butter (Shea Butter) 1.50% DC 200 / 100cs 0.50%, Vitamin E Acetate 0.10%, Nexbase 2008 FG 3.00%, Grape seed oil 1.50%, TEA 0.04%, Na-Hyaluronate (0.5%, Sodium Hyaluronic Acid) Ronate) 2 .00%, FE 6014245 0.12%, fermentation filtrate 3.00%, complex plant extract 1.00%, caramel pigment 0.01%. Looking at the manufacturing process of the moisturizing emulsion using the above raw materials, 1) put the A phase in the main kiln completely dissolved, 2) dissolve the B phase in a separate dissolution tank, and 3) put the C phase in the main kiln Moisturizing emulsion was prepared by putting and dissolving completely.

2-4. Moisturizing Cream

The moisturizing cream is A. Lactobacillus fermentation filtrate of distilled leaf extract prepared in Example 1 as purified water + 48.99% of EDTA-2Na (disodium IDT), 0.08% of D, L-Panthenol (Delpanthenol), Glycerin 5.00%, Adenosine 0.04%, Uvinul MS-40 0.04%, DPG 0.05%, 1,3-BG (butylene glycol) 13.00%, Oil-soluble licorice extract 0.05%, Keltrol-F ( Keltroel F) 0.06%, B-Phase Macadamia Nut Oil 0.40%, Beeswax 2.10%, Lanette O 2.00%, Arlacel 60V 0.40%, Arlacel 165 1.40%, Tween 60V 0.80%, Spectrastat 0.30%, Nexbase 2008 FG 2.50%, Tegocare 450 1.00%, Olivoil Glutinate 1.20%, Shea Butter (Shea Butter) 1.60%, Vitamin E Acetate 0.10%, CEH 9.00%, KF 96 (100cs) 1.00%, DC 556 0.70%, Neosil ES 7.00%, C phase FE 6014245 0.12%, composite plant extract 1.00%, caramel pigment 0.03%. Looking at the manufacturing process of the moisturizing cream using the above raw materials, 1) put the A phase in the main kiln and completely dissolve it, 2) dissolve the B phase in a separate dissolution tank, and 3) put the C phase in the main kiln Moisturizing cream was prepared by putting and dissolving completely.

2-5. Moisturizing Eye Cream

Moisturizing Eye Cream is A-phase Fermented Filtrate of Purified Water + Extract of Perilla Fructus Extract Prepared in Example 1 as Phase A, EDTA-2Na (Disodium IDT) 0.05%, Allantoin (Allantoin) 0.10%, MP 0.20%, Uvinul MS-40 0.05, Moist SS 14.00%, 1,3-BG (butylene glycol) 3.00%, oil-soluble licorice extract 0.05%, Keltrol-F (Keltrolf) 0.07%, Grape as B phase seed oil 1.20%, Beeswax 1.80%, Lanette O (cetearyl alcohol) 2.50%, Arlacel 60V 0.40%, Arlacel 165 1.50%, Tween 60V 0.80%, CEH 9.00%, Nexbase 2008 FG 2.00%, Tegocare 450 0.50%, Lipoid S 75-3 0.50%, Olivoil Glutinate 1.70%, Shea Butter (Shea Butter) 1.60%, Vitamin E Acetate 0.30%, PP 0.15%, Phenoxyethanol (Phenoxy Ethanol) 0.15%, Neosil ES 9.00%, as phase C, Forever-1w 0.24%, complex plant extract 1.00%, Adenosphere 2.00%, caramel pigment 0.04%. Looking at the manufacturing process of the moisturizing eye cream using the above raw materials, 1) put the A phase in the main kiln and completely dissolve it, 2) dissolve the B phase in a separate dissolution tank and put it in the main kiln, 3) the main kiln Moisturizing eye cream was prepared by dissolving it in a complete solution.

Example  3. Evaluation of atopic dermatitis improvement efficacy

3-1. Atopic dermatitis reduction effect

40 NC-Nga mice (SPF, 5 weeks, females, Japan) weighing 13-22g were separated from normal breeders who could eat water and food freely, and raised them for 12 hours. Atopic dermatitis was induced by applying dust dust mite extract (Dermatophagoides pteronyssinus crude extract, DPE) for 1 week (total 3 times) in 35 ears and back of NC / Nga mice. After the DPE treatment, the fermentation filtrate application group of Example 1, the base only after the DPE treatment, and the group treated with other atopic dermatitis treatment after the DPE treatment were further divided into a group without any treatment. Once a week, the degree of dermatitis in the ear was evaluated based on the following criteria before topical application, and the results are shown in Table 1. Looking at Table 1, it can be seen that the fermentation filtrate sample of the present invention is superior to the inflammatory treatment effect than conventional atopic dermatitis.

Evaluation criteria: no inflammation, 0; Slight inflammation or wound, 1; Moderate inflammation or sores or slight bleeding; 2; Inflammation or bleeding or ulceration or loss of ear tissue, 3.

n A week later 2 weeks later 3 weeks later 4 weeks later After DPE Treatment
Example 1 (Manufacturing Example 1) Coating Group 5 2 One 0 0 After DPE Treatment
Example 1 (Manufacturing Example 2) Coating Group 5 2 One One 0 After DPE Treatment
Example 1 (Manufacturing Example 3) Coating group 5 2 One 0 0 After DPE Treatment
Example 1 (Manufacture example 4) Coating group 5 One 0 0 0 After DPE Treatment
Example 1 (Manufacturing Example 5) Coating group 5 2 0 0 0 After DPE Treatment
Only the base is applied 5 3 3 3 3 After DPE Treatment
Group of other atopic dermatitis treatments 5 2 2 2 One No treatment 5 0 0 0 0

3-2. Ear edema reduction effect

In the experimental procedure of Example 3-1, the thickness of the ear was measured every week by using a dial caliper (KORI SEIKI MFG.Co, Japan). As a result, as shown in Table 2, the group coated with only the base after DPE treatment increased ear edema over time and reached about 70%, but the group coated with the fermentation filtrate of the present invention after DPE treatment initially Ear edema increased to about 30-50% and gradually reached zero, and showed an effect of suppressing ear edema better than conventional atopic dermatitis treatments.

 (unit : %) n A week later 2 weeks later 3 weeks later 4 weeks later After DPE Treatment
Example 1 (Manufacturing Example 1) Coating Group 5 35 22 10 2 After DPE Treatment
Example 1 (Manufacturing Example 2) Coating Group 5 41 34 21 11 After DPE Treatment
Example 1 (Manufacturing Example 3) Coating group 5 38 29 18 7 After DPE Treatment
Example 1 (Manufacture example 4) Coating group 5 30 19 9 2 After DPE Treatment
Example 1 (Manufacturing Example 5) Coating group 5 32 25 13 5 After DPE Treatment
Only the base is applied 5 55 62 65 70 After DPE Treatment
Group of other atopic dermatitis treatments 5 39 35 28 19 No treatment 5 0 0 0 0

3-3. Skin Humidity  Measure

In the experimental procedure of Example 3-1, the wetness of the back skin was measured every week by using corneometer CM820 (Courage & Khazaka, Cologne, Germany) and tewameter TM210 (Courage & Khazaka, Cologne, Germany).

As a result, as shown in Table 3, the group coated with the base only after DPE treatment decreased the moisture content of the skin over time, reaching about 20%, but the group coated with the fermentation filtrate of the present invention after DPE treatment Initially, the moisture content of the skin gradually recovered over time, and the skin moisture retention was superior to conventional atopic dermatitis treatments.

 (unit : %) n A week later 2 weeks later 3 weeks later 4 weeks later After DPE Treatment
Example 1 (Manufacturing Example 1) Coating Group 5 52 58 59 60 After DPE Treatment
Example 1 (Manufacturing Example 2) Coating Group 5 53 55 57 59 After DPE Treatment
Example 1 (Manufacturing Example 3) Coating group 5 55 57 60 63 After DPE Treatment
Example 1 (Manufacture example 4) Coating group 5 57 58 62 64 After DPE Treatment
Example 1 (Manufacturing Example 5) Coating group 5 52 54 55 56 After DPE Treatment
Only the base is applied 5 52 39 31 25 After DPE Treatment
Group of other atopic dermatitis treatments 5 50 47 52 55 No treatment 5 70 68 65 62

Example  4. Evaluation of improvement efficacy of skin pigmentation

As a whitening experiment, samples containing the cedar leaf fermentation filtrate according to the present invention and competitor products A and B were applied to the face of 20 test subjects (1 group of 10 testers). That is, two group groups were formed, and the sample of the present invention was applied to the right side of each group group's face twice a day for one consecutive month. In the same manner, competitor product A was applied to the left of the experimenter's face in one group of the group, and competitor product B was applied to the left of the experimenter's face in the other group. After completion of the experiment, the skin color of the applied areas on both sides of the face was analyzed by image analysis. Looking at the following Table 4, the sample of the present invention was found to be lower than the competitors A, B, and the whitening effect is excellent.

One 2 3 4 5 6 7 8 9 10 Competitor A Products 3.5 3.0 3.0 3.5 4.0 3.5 3.5 3.5 3.0 3.0 Competitor B Product 3.0 2.5 2.5 3.0 2.5 2.0 2.0 2.5 2.0 2.5 Sample of the Invention 1.5 2.0 2.0 2.0 2.0 1.5 2.0 2.0 2.5 2.0

Claims (14)

It contains a fermentation filtrate produced by fermenting the extract of Ledum palustre var. Diversipilosum with lactic acid bacteria,
The lactobacillus is Enterococcus faecium , Enterococcus faecalis , Lactobacillus plantarum , Lactobacillus plantmentum , Lactobacillus fermentum , Lactobacillus lactis , Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus sakei , Lactobacillus brevis , Lactobacillus graminis , Lactobacillus graminis , Leuconostoc citreum , Leuconostoc citreum , ( Weissella hellenica ), Lactococcus garvieae , Pediococcus pentosaceus , Pediococcus acidilactici , Bifidobacterium animalis , and Bifidobacterium Infante guneu consisting of teeth (Bifidobacterium infantis) Since it is two or more species selected,
Cosmetic composition for improving and whitening atopic dermatitis.
delete The method of claim 1, wherein the Enterococcus par when Titanium (Enterococcus faecium) is at least one selected from the group consisting of Enterococcus par when Titanium (Enterococcus faecium) ATCC 19634 or Enterococcus par when Titanium (Enterococcus faecium) ATCC 6057,
The enterococcus faecalis is Enterococcus faecalis ATCC 6057,
The Lactobacillus and Bacillus Planta room (Lactobacillus plantarum) is Lactobacillus Planta room (Lactobacillus plantarum) ATCC 10241, or Lactobacillus Planta room (Lactobacillus plantarum) one or more selected from the NCTC 7220,
The Lactobacillus and buffer lactofermentum (Lactobacillus fermentum) is Lactobacillus buffer lactofermentum (Lactobacillus fermentum) ATCC 14931, or Lactobacillus buffer lactofermentum (Lactobacillus fermentum) one or more selected from NCDO 1750,
The Lactobacillus and lock teeth (Lactobacillus lactis) is Lactobacillus lactis (Lactobacillus lactis) ATCC 11454, or Lactobacillus lactis (Lactobacillus lactis) one or more selected from the group consisting of NCIB 8586,
The Lactobacillus ramno a suspension (Lactobacillus rhamnosus) is at least one selected from the group consisting of Lactobacillus ramno suspension (Lactobacillus rhamnosus) ATCC 7469, or Lactobacillus ramno suspension (Lactobacillus rhamnosus) KCTC 3326,
The Lactobacillus and Bacillus four K (Lactobacillus sakei) is at least one selected from the group consisting of Lactobacillus four K (Lactobacillus sakei) ATCC 31063, or Lactobacillus four K (Lactobacillus sakei) ATCC 15521,
The Lactobacillus brevis is Lactobacillus brevis ATCC 15521,
The Lactobacillus graminis is Lactobacillus graminis ATCC 51150 and the Lactobacillus graminis is Lactobacillus graminis ATCC 51150,
The Leuconostoc citreum is Leuconostoc citreum ATCC 49370, the Leuconostoc citreum is Leuconostoc citreum ATCC 49370,
And the by-Cellar helre NIKA (Weissella hellenica) is by Cellar helre NIKA (Weissella hellenica) ATCC 51523,
The Lactococcus garvieae is selected from the group consisting of Lactococcus garvieae CCUG 11672, Lactococcus garvieae ATCC 11454, or Lactococcus garvieae LMG 8162 One or more,
The Pediococcus pentosaceus is Pediococcus pentosaceus ATCC 33316, the Pediococcus pentosaceus is Pediococcus pentosaceus ATCC 33316,
Wherein the Pediococcus acidilactici is Pediococcus acidilactici ATCC 33314, the Pediococcus acidilactici is Pediococcus acidilactici ATCC 33314,
The Bifidobacterium animalis is Bifidobacterium animalis ATCC 27672,
The Bifidobacterium Infante tooth (Bifidobacterium infantis) is Bifidobacterium Infante tooth (Bifidobacterium infantis) ATCC15697 of the cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition comprises bacteriocin produced by lactic acid bacteria.
According to claim 1, wherein the lactic acid bacteria Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus rhamnosus ( Lactobacillus) rhamnosus ), Lactococcus garvieae ), and Pediococcus pentosaceus pentosaceus ) 2 or more types of cosmetic composition selected from the group consisting of.
According to claim 5, The Lactobacillus plantarum ( Lactobacillus plantarum) is Lactobacillus Planta Room (Lactobacillus plantarum ) at least one selected from ATCC 10241, or Lactobacillus plantarum NCTC 7220,
The Lactobacillus < RTI ID = 0.0 > rhamnosus) is Lactobacillus ramno suspension (Lactobacillus rhamnosus) ATCC 7469, or Lactobacillus ramno suspension (Lactobacillus rhamnosus ) one or more selected from KCTC 3326,
Lactococcus garvieae) it is Lactococcus (in the non-teaching Lactococcus garvieae) CCUG 11672, (Lactococcus in Lactococcus teaching ratio garvieae ) ATCC 11454, or Lactococcus garvieae ) at least one selected from LMG 8162,
Pediococcus pentosaceus pentosaceus ) is Pediococcus pentosaceus ( Pediococcus pentosaceus ) ATCC 33316 cosmetic composition.
The method of claim 1 wherein the lactic acid bacteria is Lactobacillus Planta room (Lactobacillus plantarum) ATCC 10241, Lactobacillus ramno suspension (Lactobacillus rhamnosus ) ATCC 7469 or Pediococcus pentosaceus pentosaceus) as in any one of bacteria and Lactococcus teaching ratio selected from ATCC 33316 (Lactococcus garvieae ) ATCC 11454, or Lactococcus garvieae ) Cosmetic composition of any one selected from LMG 8162.
The cosmetic composition according to claim 1, wherein the extract of cedar leaves is a nano extract containing nanoparticles of cedar leaves.
The cosmetic composition of claim 8, wherein the nanoparticles of the cedar leaf are homogenized particles in the range of 100 to 500 nm.
The cosmetic composition according to claim 1, wherein the cosmetic composition comprises one formulation selected from the group consisting of softening agents, nutrition lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, ≪ / RTI >
Duhyang lobe (Ledum palustre var. diversipilosum) to prepare a fennel leaf nano extract containing nanoparticles;
Mixing the cedar leaf nano extract into the lactic acid bacteria culture medium; And
Enterococcus on the lactic acid bacteria culture medium ( Enterococcus faecium), Enterococcus faecalis (Enterococcus faecalis ), Lactobacillus plantarum , Lactobacillus permentum fermentum , Lactobacillus lactis , Lactobacillus rhamnosus , Lactobacillus < RTI ID = 0.0 > sakei , Lactobacillus brevis , Lactobacillus < RTI ID = 0.0 > graminis , Leuconostoc citreum , Weissella < RTI ID = 0.0 > hellenica), Lactococcus taught rain (Lactococcus garvieae), Peddie Oh Caucus pen Tosa three mouse (Pediococcus pentosaceus), Peddie Oh caucus in Sidi rakti City (Pediococcus acidilactici), Bifidobacterium No Marlies (Bifidobacterium animalis) and Bifidobacterium Infante Tees (Bifidobacterium infantis ) comprising the step of inoculating and fermenting two or more lactic acid bacteria selected from the group consisting of, the method of producing a cosmetic composition of claim 1.
12. The method of claim 11, wherein the temperature of the lactic acid bacteria in culture is usually 25 ℃ to 40 ℃, the incubation time is 7 hours to 40 hours, the lactic acid bacteria is used to be concentrated to 10 ¹⁰ to 10 ¹¹ CFU / ml Manufacturing method.
12. The method of claim 11, wherein the cedar leaf nanoparticles are homogenized particles in the range of 100 to 500 nm.
The method according to claim 11, wherein the extract of the cedar leaf nanocomposite is contained in 1 to 2% by weight compared to the lactic acid bacteria culture medium.