KR102298782B1 - Polygonum multiflorum Thunberg fermented Extract composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a fermented sewage extract composition and a method for preparing the same, and more particularly, to a fermented sewage extract composition and a method for preparing the same, which exhibit remarkably excellent antioxidative effects due to fermentation through a predetermined strain.

Description

Sewage fermentation extract composition and manufacturing method thereof {Polygonum multiflorum Thunberg fermented Extract composition and manufacturing method thereof}

The present invention relates to a fermented sewage extract composition and a method for preparing the same, and more particularly, to a fermented sewage extract composition and a method for preparing the same, which exhibit remarkably excellent antioxidative effects due to fermentation through a predetermined strain.

Recently, with the development of research centered on natural plants, interest in the physiologically active substances they have is increasing. Since bioactive substances are high value-added substances that show remarkable activity in small amounts, research and development of new substances are being actively carried out.

In particular, safety issues for some of the artificial synthetic materials are raised, and interest in natural bioactive materials is expected to increase as people's interest and desire for health increase. Therefore, in research fields such as food science, nutrition, and pharmaceuticals, the biological activity of various compounds present in various natural plants is evaluated, and among them, research on antioxidant activity is focused.

As oxides caused by oxidative stress in living things are found to be the cause of various chronic degenerative diseases such as aging, cancer, and arteriosclerosis, the value of natural antioxidants involved in controlling them is being highlighted.

In general, antioxidants are known as beneficial ingredients for the prevention of aging by inhibiting the oxidation of cell membranes in the human body. There is a problem in using it as an added additive, so research for extraction and separation of natural antioxidants is being actively conducted.

Conventionally, antioxidative properties were improved by filtering and concentrating the extract obtained by extracting defatted rice bran with a mixture of water and ethanol, and then preparing an antioxidant, but there was a problem in that antioxidant properties could not be expressed to a desired level.

Accordingly, there is an urgent need to study a substance capable of exhibiting a remarkably excellent effect of antioxidant properties.

KR 10-2004-0063291A (published on: 2004.07.14.)

The present invention has been devised to solve the above-described problems, and an object of the present invention is to provide a composition for extracting fermented sewage which exhibits a remarkably excellent effect in antioxidant properties due to fermentation through a predetermined strain, and a method for preparing the same.

In order to solve the above problems, the present invention includes one selected from the group consisting of sewage water, C1 to C4 alcohols having a concentration of 15 to 45%, and C1 to C4 alcohols having a concentration of 55 to 85%. The sewage extract extracted through the first solvent, comprising at least one selected from the group consisting of Lactobacillus helveticus, Streptococcus thermophilus and Bifidobacterium longum It provides a sewage fermentation extract composition comprising a; sewage fermentation extract fermented with a strain as an active ingredient.

According to an embodiment of the present invention, when the first solvent is water, and the strain is Lactobacillus helveticus or Bifidobacterium longum, 30 ~ 420 It may be included at a concentration of μg/ml.

In addition, when the first solvent is an alcohol of C1 to C4 having a concentration of 15 to 45%, and the strain is Lactobacillus helveticus, the sewage fermentation extract is used at a concentration of 1 to 210 μg/ml. may include

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 15 to 45%, and the strain is Streptococcus thermophilus, the fermented sewage extract is used at a concentration of 30 to 60 μg/ml. may include

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 15 to 45%, and the strain is Bifidobacterium longum, the fermented sewage extract is used at a concentration of 30 to 150 μg/ml. may include

In addition, when the first solvent is an alcohol of C1 to C4 having a concentration of 55 to 85%, and the strain is Lactobacillus helveticus, the sewage fermentation extract is used at a concentration of 3 to 8 μg/ml. may include

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 55 to 85%, and the strain is Bifidobacterium longum, the fermented sewage extract is used at a concentration of 80 to 130 μg/ml. may include

In addition, the fermentation may be performed at a temperature of 25 to 45° C. for 35 to 61 hours.

In addition, the Lactobacillus helveticus may have an accession number KACC91148, the Streptococcus thermophilus may have an accession number KACC91147, and the Bifidobacterium longum may be The accession number may be KACC91563.

On the other hand, the present invention provides a first solvent comprising one selected from the group consisting of (1) sewage water, a C1-C4 alcohol having a concentration of 15 to 45%, and a C1-C4 alcohol having a concentration of 55 to 85%. Extracting through to prepare a sewage extract; (2) Fermentation of the sewage extract into a strain containing at least one selected from the group consisting of Lactobacillus helveticus, Streptococcus thermophilus and Bifidobacterium longum to prepare a sewage fermentation extract; (3) preparing a sewage fermentation extract composition comprising the sewage fermentation extract as an active ingredient; provides a method for preparing a sewage fermentation extract composition comprising.

On the other hand, the present invention provides a cosmetic composition comprising the above-mentioned sewage fermentation extract composition; as an active ingredient.

On the other hand, the present invention provides a health supplement comprising; as an active ingredient;

On the other hand, the present invention provides a pharmaceutical composition comprising the above-mentioned sewage fermentation extract composition; as an active ingredient.

The fermented sewage extract composition of the present invention and a method for producing the same can express a remarkably excellent effect of antioxidant properties due to fermentation through a predetermined strain.

1 to 3 are graphs of DPPH antioxidant activity test results according to the extraction solvent and fermentation strain of Hsuo, and,
4 to 6 are graphs of the results of the ABTS antioxidant experimentation according to the extraction solvent and fermentation strain of Hawthorn.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art to which the present invention pertains can easily implement them. The present invention may be embodied in many different forms and is not limited to the embodiments described herein.

The sewage fermentation extract composition according to an embodiment of the present invention is implemented by including, as an active ingredient, a sewage sewage extract obtained by extracting sewage from a first solvent, and a sewage fermentation extract fermented with a strain.

First, the sewage extract will be described.

The sewage extract is prepared by extracting sewage through a first solvent.

In this case, the first solvent may be used without limitation as long as it is a solvent commonly used in the art, and may preferably include at least one selected from the group consisting of water and an organic solvent, more preferably water and It may include at least one selected from the group consisting of C1 to C4 alcohols, and more preferably water, C1 to C4 alcohols having a concentration of 15 to 45%, and C1 to C4 alcohols having a concentration of 55 to 85%. It may include at least one selected from the group consisting of alcohols, and more preferably from the group consisting of water, C1-C4 alcohols having a concentration of 15 to 45%, and C1-C4 alcohols having a concentration of 55 to 85%. may include one selected from the group consisting of water, C1 to C4 alcohol having a concentration of 20 to 40%, and C1 to C4 alcohol having a concentration of 60 to 80%. have.

In addition, the extraction method and conditions are not particularly limited in the present invention, as they can be used without limitation as long as the extraction methods and conditions for natural vegetable materials that can be commonly used in the art are used.

On the other hand, before preparing the sewage extract, the sewage may be pre-treated so that the production of the sewage extract is easier.

The pretreatment may be performed by washing with water, pulverizing, etc., and the method and conditions for washing and pulverizing can be used without limitation as long as the methods and conditions for washing and pulverizing are commonly used in the art. It is not particularly limited.

Next, the fermented sewage extract will be described.

The fermented sewage extract may be prepared by fermenting the sewage extract into a strain.

The strain is at least one selected from the group consisting of Lactobacillus helveticus, Streptococcus thermophilus and Bifidobacterium longum, preferably Lactobacillus helveticus (Lactobacillus helveticus), Streptococcus thermophilus (Streptococcus thermophilus), and may be one selected from the group consisting of Bifidobacterium longum (Bifidobacterium longum).

In this case, the Lactobacillus helveticus may have an accession number of KACC91148, and the Streptococcus thermophilus may have an accession number of KACC91147, and the Bifidobacterium longum) The accession number may be KACC91563.

The fermented sewage extract composition according to the present invention contains, as an active ingredient, a fermented sewage extract obtained by fermenting the sewage extract through the above-mentioned strain, thereby exhibiting a remarkably excellent effect of antioxidant properties.

Next, a description will be given of the fermented sewage extract composition.

As described above, the fermented sewage extract composition includes the fermented sewage extract as an active ingredient.

In this case, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition may be different depending on the type of the first solvent and the strain.

Specifically, when the first solvent is water, and the strain is Lactobacillus helveticus or Bifidobacterium longum, the sewage fermentation extract composition comprises 30 to 420 of the sewage fermentation extract. At a concentration of μg/ml, preferably, it may be included at a concentration of 40 to 400 μg/ml. If the first solvent is water and the strain is Lactobacillus helveticus or Bifidobacterium longum, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is 30 If it is less than μg/ml or exceeds 420 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 15 to 45%, and the strain is Lactobacillus helveticus, the sewage fermentation extract composition contains 1 to 210 of the sewage fermentation extract. At a concentration of μg/ml, preferably, it may be included at a concentration of 2 to 200 μg/ml. If the first solvent is a C1-C4 alcohol having a concentration of 15 to 45%, and the strain is Lactobacillus helveticus, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is If it is less than 2 μg/ml, or exceeds 200 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 15 to 45%, and the strain is Streptococcus thermophilus, the sewage fermentation extract composition contains 30 to 60% of the sewage fermented extract. It may be included at a concentration of μg/ml, preferably at a concentration of 35 to 55 μg/ml. If the first solvent is a C1-C4 alcohol having a concentration of 15 to 45%, and the strain is Streptococcus thermophilus, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is If it is less than 30 μg/ml, or exceeds 60 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

In addition, when the first solvent is a C1 to C4 alcohol having a concentration of 15 to 45%, and the strain is Bifidobacterium longum, the sewage fermentation extract composition is 30 ~ 150 At a concentration of ㎍ / ㎖, preferably 40 ~ 　 130㎍ / ㎖ may be included at a concentration. If the first solvent is a C1-C4 alcohol having a concentration of 15 to 45%, and the strain is Bifidobacterium longum, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is If it is less than 30 μg/ml, or exceeds 150 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

In addition, when the first solvent is an alcohol of C1 to C4 having a concentration of 55 to 85%, and the strain is Lactobacillus helveticus, the sewage fermentation extract composition contains 3 to 8 of the sewage fermentation extract. It may be included at a concentration of μg/ml, preferably at a concentration of 4 to 7 μg/ml. If the first solvent is a C1-C4 alcohol having a concentration of 55 to 85%, and the strain is Lactobacillus helveticus, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is If it is less than 3 μg/ml, or exceeds 8 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

And, when the first solvent is a C1 to C4 alcohol having a concentration of 55 to 85%, and the strain is Bifidobacterium longum, the sewage fermentation extract composition contains 80 to 130 of the sewage fermentation extract. It may be included at a concentration of μg/ml, preferably at a concentration of 85 to 120 μg/ml. If the first solvent is a C1-C4 alcohol having a concentration of 55 to 85%, and the strain is Bifidobacterium longum, the concentration of the sewage fermentation extract provided in the sewage fermentation extract composition is If it is less than 80 μg/ml, or exceeds 130 μg/ml, there may be a problem in that antioxidant activity cannot be expressed at a desired level.

On the other hand, the sewage fermentation extract composition, in addition to the sewage fermentation extract, a second solvent; may further include.

The second solvent can be used without limitation as long as it is a solvent that can be used in cosmetic compositions, health supplements, and pharmaceutical compositions to be described later in the art, preferably water, C1 having a concentration of 15 to 45% may include at least one selected from the group consisting of C4 alcohol and C1 to C4 alcohol having a concentration of 55 to 85%, more preferably water, C1 to C4 alcohol having a concentration of 15 to 45%, and concentration It may include one selected from the group consisting of C1 to C4 alcohols having 55 to 85%.

The sewage fermentation extract composition according to the present invention comprises the steps of: (1) extracting sewage water through a first solvent to prepare a sewage sewage extract; (2) preparing a fermented sewage extract by fermenting the sewage extract into a strain; (3) preparing a sewage fermentation extract composition comprising the sewage fermentation extract as an active ingredient; is prepared including.

At this time, the extraction of step (1) can be used without limitation as long as it is an extraction condition that can be used commonly in the art, and is preferably treated at 60 to 80° C. for 3 to 5 hours, and rested for 3 to 5 hours. Repeating the process 2 to 5 times, more preferably treating at 65 to 75° C. for 3.5 to 4.5 hours, and resting for 3.5 to 4.5 hours may be repeated 2 to 4 times, but is not limited thereto .

In addition, the fermentation of step (2) can be used without limitation as long as it is a fermentation condition that can be used commonly in the art, preferably at a temperature of 25 to 45° C. for 48 to 96 hours, more preferably at a temperature of 30 to 40 It can be carried out at ℃ for 55 to 89 hours.

On the other hand, the present invention provides a cosmetic composition, a health supplement and a pharmaceutical composition comprising the above-mentioned sewage fermented extract composition as an active ingredient, respectively.

The type of the health supplement is not limited as long as it is a conventional health supplement in the art, and for example, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, etc. There are dairy products, various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes, etc. include

When the health supplement is a beverage composition, there is no particular limitation on the liquid component except that it contains the fermented sewage extract according to the present invention, and it may contain various flavoring agents or natural carbohydrates as additional components as in a conventional beverage. .

In general, the amount of the fermented sewage extract included in the health supplements may be included in an amount of 0.01 to 20% by weight, preferably 0.01 to 10% by weight of the total food weight. In addition, in the case of long-term intake for the purpose of health and hygiene or health control, it may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

In addition, when formulated as a cosmetic composition, the content of the fermentation extract composition is 0.0001 to 10% by weight, preferably 0.01 to 5.0% by weight, based on the total weight of the cosmetic composition. The content of the fermented sewage extract composition is preferably equal to or higher than the minimum value to achieve the skin damage improvement effect caused by minimal ultraviolet rays, and in consideration of the decrease in feeling of use due to excessive addition and the possibility of application to various formulations, the fermented sewage extract composition is It is preferably below the maximum. In this case, the content of the sewage fermentation extract composition may be appropriately adjusted within the above range according to the content of the ingredients contained in the formulation or cosmetic composition.

The fermented sewage extract composition of the present invention and a method for producing the same can express a remarkably excellent effect of antioxidant properties due to fermentation through a predetermined strain.

The present invention will be described in more detail through the following examples, but the following examples are not intended to limit the scope of the present invention, which should be construed to aid understanding of the present invention.

[Example]

Example 1: Preparation of Sewage Fermentation Extraction Composition

(1) Preparation of sewage extract

After extracting sewage water with the first solvent into water, treating at 70° C. for 4 hours, and resting for 4 hours 4 times, the first solvent used for extraction is removed through vacuum filtration to extract sewage. was prepared.

(2) Preparation of fermented sewage extract

Bifidobacterium longum (Bifidobacterium longum) strain (Accession No.: KACC91563) was inoculated into the prepared sewage extract and fermented at 35±2° C. for 72 hours to obtain a sewage fermented extract.

(3) Preparation of a composition for extracting sewage fermented water

The prepared sewage fermentation extract was diluted in distilled water as a second solvent to prepare a sewage fermentation extract composition so that the concentration of the sewage fermentation extract was 40 μg/ml.

Examples 2 to 36

The same procedure as in Example 1, except that the type of the first solvent and the second solvent, the type of strain, and the concentration of the sewage fermented extract in the sewage fermentation extract composition were different, as shown in Tables 1 and 2 below. was prepared.

Comparative Examples 1 to 12

In the same manner as in Example 1, except that the sewage extract is mixed with the second solvent without fermenting the sewage extract, the types of the first solvent and the second solvent and the concentration of the sewage extract of the sewage fermented extract composition are different. As shown in Tables 1 and 2 below, a composition for extracting sewage ferment was prepared.

At this time, Lactobacillus helveticus, which is a strain used below, has an accession number of KACC91148, and Streptococcus thermophilus has an accession number of KACC91147.

division first/second solvent
Kinds strain type density
(μg/ml) division first/second solvent
Kinds strain type density
(μg/ml) Example
One water Bifidobacterium longum 40 Example
13 Concentration 30%
spirits Bifidobacterium longum 40 Example
2 water Bifidobacterium longum 100 Example
14 Concentration 30%
spirits Bifidobacterium longum 100 Example
3 water Bifidobacterium longum 200 Example
15 Concentration 30%
spirits Bifidobacterium longum 200 Example
4 water Bifidobacterium longum 400 Example
16 Concentration 30%
spirits Bifidobacterium longum 400 Example
5 water Lactobacillus helveticus 40 Example
17 Concentration 30%
spirits Lactobacillus helveticus 40 Example
6 water Lactobacillus helveticus 100 Example
18 Concentration 30%
spirits Lactobacillus helveticus 100 Example
7 water Lactobacillus helveticus 200 Example
19 Concentration 30%
spirits Lactobacillus helveticus 200 Example
8 water Lactobacillus helveticus 400 Example
20 Concentration 30%
spirits Lactobacillus helveticus 400 Example
9 water Streptococcus thermophilus 40 Example
21 Concentration 30%
spirits Streptococcus thermophilus 40 Example
10 water Streptococcus thermophilus 100 Example
22 Concentration 30%
spirits Streptococcus thermophilus 100 Example
11 water Streptococcus thermophilus 200 Example
23 Concentration 30%
spirits Streptococcus thermophilus 200 Example
12 water Streptococcus thermophilus 400 Example
24 Concentration 30%
spirits Streptococcus thermophilus 400

division first/second solvent
Kinds strain type density
(μg/ml) division first/second solvent
Kinds strain type density
(μg/ml) Example
25 Concentration 70%
spirits Bifidobacterium longum 40 comparative example
One water - 40 Example
26 Concentration 70%
spirits Bifidobacterium longum 100 comparative example
2 water - 100 Example
27 Concentration 70%
spirits Bifidobacterium longum 200 comparative example
3 water - 200 Example
28 Concentration 70%
spirits Bifidobacterium longum 400 comparative example
4 water - 400 Example
29 Concentration 70%
spirits Lactobacillus helveticus 40 comparative example
5 Concentration 30%
spirits - 40 Example
30 Concentration 70%
spirits Lactobacillus helveticus 100 comparative example
6 Concentration 30%
spirits - 100 Example
31 Concentration 70%
spirits Lactobacillus helveticus 200 comparative example
7 Concentration 30%
spirits - 200 Example
32 Concentration 70%
spirits Lactobacillus helveticus 400 comparative example
8 Concentration 30%
spirits - 400 Example
33 Concentration 70%
spirits Streptococcus thermophilus 40 comparative example
9 Concentration 70%
spirits - 40 Example
34 Concentration 70%
spirits Streptococcus thermophilus 100 comparative example
10 Concentration 70%
spirits - 100 Example
35 Concentration 70%
spirits Streptococcus thermophilus 200 comparative example
11 Concentration 70%
spirits - 200 Example
36 Concentration 70%
spirits Streptococcus thermophilus 400 comparative example
12 Concentration 70%
spirits - 400

<Experimental Example 1>

Examples 1 to 36 and Comparative Examples 1 to 12 were measured/evaluated for the following items and shown in FIGS. 1 to 3 .

1. Measurement of DPPH free radical scavenging activity

For the sewage fermentation extract compositions prepared according to Examples 1 to 36 and Comparative Examples 1 to 12, the measurement of DPPH free radical scavenging activity was evaluated.

1 ml of the sewage fermentation extract composition prepared according to Examples 1 to 36 and Comparative Examples 1 to 12 was prepared, and for the BHT solution used as a standard, 10 mg of BHT was diluted with 1 ml of methanol in Example 1 ~ 36 and Comparative Examples 1 to 12 were prepared at the same concentration as 1 ml each. The DPPH solution was prepared by mixing 25 ml of DPPH 0.002 gdp methanol.

Then, in a 96 well plate, a group in which 80 mL of BHT solution and methanol were mixed, 80 mL each of BHT and DPPH solution, 80 mL each of sample and DPPH solution, and methanol and DPPH solution Separately and dispense into a group in which 80 mL of each was mixed, 160 mL of methanol alone, 80 mL of distilled water and DPPH solution, and 80 mL of distilled water and methanol, respectively. After wrapping with foil to prevent reaction, it was left at room temperature for 30 minutes. Absorbance was measured at 517 nm using an ELISA reader.

Absorbance was measured at 517 nm using an absorbance measuring instrument (ELISA reader).

And, for each Example having the same concentration as Comparative Examples 1 to 12 among Examples 1 to 36 through the measured absorbance, the degree of improvement in the DPPH free radical scavenging activity compared to Comparative Examples 1 to 12 was calculated using the following Equation 1 , and the results are shown in FIGS. 1 to 3 .

[Equation 1]

In the mathematical, R, R _A, R _B is a radical scavenging activity of the composition of the radical scavenging activity, in Comparative Examples of the composition of the respective radical scavenging rate, the embodiment.

As a result of the measurement, the DPPH free radical scavenging ability of Examples 1 to 8 among Examples 1 to 12 using water as the first solvent as shown in FIG. 1 was remarkably excellent, and as shown in FIG. The DPPH free radical scavenging ability of Examples 13, 14, 17 to 19 and 21 among Examples 13 to 24 using alcohol having a concentration of 30% was remarkably excellent, and as shown in FIG. 3, alcohol having a concentration of 70% as the first solvent As the DPPH free radical scavenging ability of Example 26 was remarkably excellent among Examples 25 to 36 using

Example 37-Example 63

It was prepared in the same manner as in Example 1, except that ethanol having a concentration of 50% was used as the second solvent, and the type of the first solvent and the second solvent, the type of the strain, the concentration of the sewage fermentation extract in the sewage fermentation extract composition, etc. Alternatively, as shown in Tables 3 and 4 below, a composition for extracting sewage fermented water was prepared.

Comparative Examples 13 to 22

It was prepared in the same manner as in Comparative Example 1, except that ethanol having a concentration of 50% was used as the second solvent, and the type of the first solvent and the second solvent, the type of the strain, the concentration of the sewage fermentation extract in the sewage fermentation extract composition, etc. Alternatively, as shown in Tables 3 and 4 below, a composition for extracting sewage fermented water was prepared.

At this time, Lactobacillus helveticus, which is a strain used below, has an accession number of KACC91148, and Streptococcus thermophilus has an accession number of KACC91147.

division first/second solvent
Kinds strain type density
(μg/ml) division first/second solvent
Kinds strain type density
(μg/ml) Example
37 water Bifidobacterium longum 2 Example
46 Concentration 30%
spirits Bifidobacterium longum 2 Example
38 water Bifidobacterium longum 5 Example
47 Concentration 30%
spirits Bifidobacterium longum 5 Example
39 water Bifidobacterium longum 10 Example
48 Concentration 30%
spirits Bifidobacterium longum 10 Example
40 water Lactobacillus helveticus 2 Example
49 Concentration 30%
spirits Lactobacillus helveticus 2 Example
41 water Lactobacillus helveticus 5 Example
50 Concentration 30%
spirits Lactobacillus helveticus 5 Example
42 water Lactobacillus helveticus 10 Example
51 Concentration 30%
spirits Lactobacillus helveticus 10 Example
43 water Streptococcus thermophilus 2 Example
52 Concentration 30%
spirits Streptococcus thermophilus 2 Example
44 water Streptococcus thermophilus 5 Example
53 Concentration 30%
spirits Streptococcus thermophilus 5 Example
45 water Streptococcus thermophilus 10 Example
54 Concentration 30%
spirits Streptococcus thermophilus 10

division first/second solvent
Kinds strain type density
(μg/ml) division first/second solvent
Kinds strain type density
(μg/ml) Example
55 Concentration 70%
spirits Bifidobacterium longum 2 comparative example
13 water - 2 Example
56 Concentration 70%
spirits Bifidobacterium longum 5 comparative example
14 water - 5 Example
57 Concentration 70%
spirits Bifidobacterium longum 10 comparative example
15 water - 10 Example
58 Concentration 70%
spirits Lactobacillus helveticus 2 comparative example
16 Concentration 30%
spirits - 2 Example
59 Concentration 70%
spirits Lactobacillus helveticus 5 comparative example
17 Concentration 30%
spirits - 5 Example
60 Concentration 70%
spirits Lactobacillus helveticus 10 comparative example
18 Concentration 30%
spirits - 10 Example
61 Concentration 70%
spirits Streptococcus thermophilus 2 comparative example
19 Concentration 70%
spirits - 2 Example
62 Concentration 70%
spirits Streptococcus thermophilus 5 comparative example
20 Concentration 70%
spirits - 5 Example
63 Concentration 70%
spirits Streptococcus thermophilus 10 comparative example
21 Concentration 70%
spirits - 10

<Experimental Example 2>

Examples 37 to 63 and Comparative Examples 13 to 21 were measured/evaluated for the following items and shown in FIGS. 4 to 6 .

1. Measurement of ABTS radical scavenging activity

ABTS radical scavenging activity was evaluated for the fermented sewage extract compositions prepared according to Examples 37 to 63 and Comparative Examples 13 to 21.

Specifically, 7 mM ABTS and 140 mM potassium persulfate solution were mixed, left in the dark for 24 hours, and then diluted with ethanol at a concentration of 50% so that the absorbance value at 734 nm was 0.7 ± 0.02. , Examples 37 to 63 and Comparative Examples 13 to 21 by adding 50 μL of the fermentation extract composition prepared in accordance with the absorbance was measured exactly 3 minutes later, ABTS radical scavenging activity (%) by the following Equation 2 was converted to , ABTS radical scavenging activity (%) was measured by measuring the absorbance reduction rate relative to the control.

[Equation 2]

ABTS radical scavenging activity (%) = {1- (sample absorbance / control absorbance)} × 100

And, for each Example having the same concentration as Comparative Examples 13 to 21 among Examples 37 to 63, the degree of improvement in ABTS radical scavenging activity compared to Comparative Examples 13 to 21 is shown in FIGS. 4 to 6 .

As a result of the measurement, as shown in FIG. 4, among Examples 37 to 45 using water as the first solvent, the ABTS radical scavenging ability of Example 41 was remarkably excellent, and as shown in FIG. 5, the concentration of 30% as the first solvent The ABTS radical scavenging ability of Examples 49 to 51 among Examples 46 to 54 using the alcohol of As the ABTS radical scavenging ability of 59 is remarkably excellent, it can be confirmed that the antioxidant properties are remarkably excellent.

Hereinafter, although it will be shown with respect to a manufacturing example comprising the fermented sewage extract composition according to the present invention as an active ingredient, the present invention is only intended to be described in detail, and is not limited thereto.

Preparation Example 1: Preparation of a health supplement comprising the fermented sewage extract composition as an active ingredient

Sauerkraut fermented extract composition 200 mg

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B 1 0.13 mg

Vitamin B 2 0.15 mg

Vitamin B 6 0.5mg

0.2 μg of vitamin B 12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Preparation Example 2: Preparation of a cosmetic composition comprising a sewage fermentation extract composition as an active ingredient

Sauerkraut fermentation extract composition: 1% by weight

Lipophilic glycerin monostearate: 2% by weight

Cetearyl alcohol: 2% by weight

Stearic acid: 1.5 wt%

Polysorbate 60: 1.5 wt%

Sorbitan Stearate: 0.6 wt%

Hydrogenated polyisobutene: 1% by weight

Squalane: 3% by weight

Mineral oil: 5% by weight

Cyclomethicone: 5% by weight

Dimethicone: 1 wt%

Tocopherol acetate: 0.5 wt%

Glycerin: 5% by weight

Betaine: 3% by weight

Triethanolamine: 1% by weight

Xanthan Gum: 0.05% by weight

Fragrance: Appropriate amount

Preservative: Appropriate amount

Color: Appropriate amount

Distilled water: remaining

Although one embodiment of the present invention has been described above, the spirit of the present invention is not limited to the embodiments presented herein, and those skilled in the art who understand the spirit of the present invention can add components within the scope of the same spirit. , changes, deletions, additions, etc. will be able to easily suggest other embodiments, but this will also fall within the scope of the present invention.

Claims (11)

A sewage extract extracted through a first solvent containing one selected from the group consisting of water, a concentration of 15 to 45% C1 to C4 alcohol, and a concentration of 55 to 85% C1 to C4 alcohol, strepto Caucus thermophilus ( Streptococcus thermophilus , KACC91147 ) and Bifidobacterium longum ( Bifidobacterium longum , KACC91563) Fermentation extract composition. According to claim 1,
When the first solvent is water and the strain is Bifidobacterium longum (KACC91563),
Sewage fermentation extract composition comprising the sewage fermentation extract at a concentration of 30 ~ 420㎍ / ㎖. According to claim 1,
When the first solvent is a C1-C4 alcohol having a concentration of 15 to 45%, and the strain is Streptococcus thermophilus (KACC91147),
A sewage fermentation extract composition comprising the sewage fermentation extract at a concentration of 30 to 60 μg/ml. According to claim 1,
When the first solvent is a C1-C4 alcohol having a concentration of 15 to 45%, and the strain is Bifidobacterium longum (KACC91563),
Sewage fermentation extract composition comprising the sewage fermentation extract at a concentration of 30 ~ 150㎍ / ㎖. According to claim 1,
When the first solvent is a C1 to C4 alcohol having a concentration of 55 to 85%, and the strain is Bifidobacterium longum (KACC91563),
A sewage fermentation extract composition comprising the sewage fermentation extract at a concentration of 80 to 130 μg/ml. According to claim 1,
The fermentation is performed for 35 to 61 hours at a temperature of 25 ~ 45 ℃ Sewage fermentation extract composition. delete (1) Sewage is extracted through a first solvent containing one selected from the group consisting of water, C1 to C4 alcohols with a concentration of 15 to 45%, and C1 to C4 alcohols with a concentration of 55 to 85%. preparing an extract;
(2) fermenting the sewage extract into a strain containing at least one selected from the group consisting of Streptococcus thermophilus (KACC91147) and Bifidobacterium longum (KACC91563) to prepare a sewage fermented extract to do;
(3) preparing a sewage fermentation extract composition comprising the sewage fermentation extract as an active ingredient; a method for producing a sewage fermentation extract composition comprising a. A cosmetic composition comprising; the sewage fermented extract composition according to claim 1 as an active ingredient. A health supplement comprising, as an active ingredient, the fermented sewage extract composition according to claim 1 . delete

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